//classic/reporter/multiple
type: Collection
persistent identity: classic reporter multiple
display id: classic_reporter_multiple
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I13604
member: BBa_K772008
member: BBa_T1004
member: BBa_K772103
member: AnRe
member: BBa_I13607
member: BBa_K515107
member: BBa_I13605

BBa_K772103
type: ComponentDefinition
persistent identity: BBa K772103
display id: BBa_K772103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex generator
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GFP - LacZ protein generator is generated with the aim of inducing a reporting reaction aftermath of the activation of EpCAM detection system. (BBa_K772100) For more information: BBa_K772006
mutable notes: To achieve a constitutive production, pTetR promoter is added in the upstream region of the original part.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K772006
sequence annotation: BBa_R0040
sequence annotation: BBa_K772006
sequence: https://synbiohub.org/public/igem/BBa_K772103_sequence/1

BBa_R0040
type: Component
persistent identity: component2211349
display id: component2211349
version: 1
top level: BBa_K772103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K772006
type: Component
persistent identity: component2211360
display id: component2211360
version: 1
top level: BBa_K772103
access: public
definition: AnRe

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2211349
display id: annotation2211349
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
type: Range
persistent identity: range2211349
display id: range2211349
version: 1
direction: direction/forward
top level: BBa_K772103
start: 1
end: 54
orientation: inline

BBa_K772006
type: SequenceAnnotation
persistent identity: annotation2211360
display id: annotation2211360
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
component: BBa_K772006

https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
type: Range
persistent identity: range2211360
display id: range2211360
version: 1
direction: direction/reverse
top level: BBa_K772103
start: 63
end: 1269
orientation: inline

BBa_T1004
type: ComponentDefinition
persistent identity: BBa T1004
display id: BBa_T1004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test part
creator: Randy Rettberg
created: 2010-10-20T11:00:00Z
modified: 2015-09-03T11:55:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 4206
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: test of adding a composite part
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0001953
role: partType/Scar
component: BBa_B0015
component: BBa_F2620
component: BBa_T1001
component: BBa_T1002
sequence annotation: BBa_B0015
sequence annotation: BBa_T1001
sequence annotation: BBa_T1002
sequence annotation: BBa_F2620
sequence: https://synbiohub.org/public/igem/BBa_T1004_sequence/1

BBa_B0015
type: Component
persistent identity: component2377262
display id: component2377262
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_F2620
type: Component
persistent identity: component2377295
display id: component2377295
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_F2620/1

BBa_T1001
type: Component
persistent identity: component2377253
display id: component2377253
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1001/1

BBa_T1002
type: Component
persistent identity: component2377268
display id: component2377268
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1002/1

BBa_T1001
type: Component
persistent identity: component2377269
display id: component2377269
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_T1001/1

BBa_B0015
type: Component
persistent identity: component2377302
display id: component2377302
version: 1
top level: BBa_T1004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2377302
display id: annotation2377302
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377302/range2377302/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_T1004/annotation2377302/range2377302/1
type: Range
persistent identity: range2377302
display id: range2377302
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 2156
end: 2284
orientation: inline

BBa_T1001
type: SequenceAnnotation
persistent identity: annotation2377253
display id: annotation2377253
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377253/range2377253/1
component: BBa_T1001

https://synbiohub.org/public/igem/BBa_T1004/annotation2377253/range2377253/1
type: Range
persistent identity: range2377253
display id: range2377253
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1
end: 52
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2377262
display id: annotation2377262
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377262/range2377262/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_T1004/annotation2377262/range2377262/1
type: Range
persistent identity: range2377262
display id: range2377262
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 890
end: 1018
orientation: inline

BBa_T1002
type: SequenceAnnotation
persistent identity: annotation2377268
display id: annotation2377268
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377268/range2377268/1
component: BBa_T1002

https://synbiohub.org/public/igem/BBa_T1004/annotation2377268/range2377268/1
type: Range
persistent identity: range2377268
display id: range2377268
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 61
end: 1018
orientation: inline

BBa_T1001
type: SequenceAnnotation
persistent identity: annotation2377269
display id: annotation2377269
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377269/range2377269/1
component: BBa_T1001

https://synbiohub.org/public/igem/BBa_T1004/annotation2377269/range2377269/1
type: Range
persistent identity: range2377269
display id: range2377269
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1027
end: 1078
orientation: inline

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2377295
display id: annotation2377295
version: 1
top level: BBa_T1004
location: https://synbiohub.org/public/igem/BBa_T1004/annotation2377295/range2377295/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_T1004/annotation2377295/range2377295/1
type: Range
persistent identity: range2377295
display id: range2377295
version: 1
direction: direction/reverse
top level: BBa_T1004
start: 1087
end: 2147
orientation: inline

BBa_K772008
type: ComponentDefinition
persistent identity: BBa K772008
display id: BBa_K772008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter device (LacZ enzyme catalyses the reaction of the cleavage of X-Gal which results with blue color change) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Cleavage site, LacZ and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: GFP
sequence annotation: Anchor-Reporter + Flag
sequence annotation: RBS
sequence annotation: TEV cleavage site
sequence annotation: Double Terminator
sequence annotation: LacZ
sequence annotation: Flag Tag
sequence: https://synbiohub.org/public/igem/BBa_K772008_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2203912
display id: annotation2203912
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1
type: Range
persistent identity: range2203912
display id: range2203912
version: 1
direction: direction/forward
top level: BBa_K772008
start: 47
end: 767
orientation: inline

Anchor-Reporter + Flag
type: SequenceAnnotation
persistent identity: annotation2203916
display id: annotation2203916
version: 1
top level: BBa_K772008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1
type: Range
persistent identity: range2203916
display id: range2203916
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 1101
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203910
display id: annotation2203910
version: 1
top level: BBa_K772008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1
type: Range
persistent identity: range2203910
display id: range2203910
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1
end: 20
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203913
display id: annotation2203913
version: 1
top level: BBa_K772008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1
type: Range
persistent identity: range2203913
display id: range2203913
version: 1
direction: direction/forward
top level: BBa_K772008
start: 768
end: 857
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203915
display id: annotation2203915
version: 1
top level: BBa_K772008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1
type: Range
persistent identity: range2203915
display id: range2203915
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1102
end: 1232
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203914
display id: annotation2203914
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1
type: Range
persistent identity: range2203914
display id: range2203914
version: 1
direction: direction/forward
top level: BBa_K772008
start: 858
end: 1101
orientation: inline

Flag Tag
type: SequenceAnnotation
persistent identity: annotation2203911
display id: annotation2203911
version: 1
top level: BBa_K772008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1
type: Range
persistent identity: range2203911
display id: range2203911
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 46
orientation: inline

BBa_I13604
type: ComponentDefinition
persistent identity: BBa I13604
display id: BBa_I13604
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter construct for constitutive YFP and inducible CFP
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 139
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter Construct with Tet operator+YFP(LVA-) reporter followed by a Lac operator +CFP(LVA-) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0420
component: BBa_I6032
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_E0420
sequence annotation: BBa_I6032
sequence: https://synbiohub.org/public/igem/BBa_I13604_sequence/1

BBa_E0420
type: Component
persistent identity: component2228624
display id: component2228624
version: 1
top level: BBa_I13604
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_I6032
type: Component
persistent identity: component2228609
display id: component2228609
version: 1
top level: BBa_I13604
access: public
definition: https://synbiohub.org/public/igem/BBa_I6032/1

BBa_R0011
type: Component
persistent identity: component2228610
display id: component2228610
version: 1
top level: BBa_I13604
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2228610
display id: annotation2228610
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228610/range2228610/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13604/annotation2228610/range2228610/1
type: Range
persistent identity: range2228610
display id: range2228610
version: 1
direction: direction/forward
top level: BBa_I13604
start: 949
end: 1002
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2228624
display id: annotation2228624
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228624/range2228624/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13604/annotation2228624/range2228624/1
type: Range
persistent identity: range2228624
display id: range2228624
version: 1
direction: direction/reverse
top level: BBa_I13604
start: 1012
end: 1889
orientation: inline

BBa_I6032
type: SequenceAnnotation
persistent identity: annotation2228609
display id: annotation2228609
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228609/range2228609/1
component: BBa_I6032

https://synbiohub.org/public/igem/BBa_I13604/annotation2228609/range2228609/1
type: Range
persistent identity: range2228609
display id: range2228609
version: 1
direction: direction/reverse
top level: BBa_I13604
start: 1
end: 940
orientation: inline

BBa_I13607
type: ComponentDefinition
persistent identity: BBa I13607
display id: BBa_I13607
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac YFP Tet CFP (Ly+Tc+)
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Reporter Construct with Lac operator+YFP(LVA+) reporter followed by a Tet operator +CFP(LVA+) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I6061
component: BBa_E0422
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0422
sequence annotation: BBa_I6061
sequence: https://synbiohub.org/public/igem/BBa_I13607_sequence/1

BBa_I6061
type: Component
persistent identity: component2228713
display id: component2228713
version: 1
top level: BBa_I13607
access: public
definition: https://synbiohub.org/public/igem/BBa_I6061/1

BBa_E0422
type: Component
persistent identity: component2228732
display id: component2228732
version: 1
top level: BBa_I13607
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_R0040
type: Component
persistent identity: component2228714
display id: component2228714
version: 1
top level: BBa_I13607
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2228714
display id: annotation2228714
version: 1
top level: BBa_I13607
location: https://synbiohub.org/public/igem/BBa_I13607/annotation2228714/range2228714/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13607/annotation2228714/range2228714/1
type: Range
persistent identity: range2228714
display id: range2228714
version: 1
direction: direction/forward
top level: BBa_I13607
start: 989
end: 1042
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2228732
display id: annotation2228732
version: 1
top level: BBa_I13607
location: https://synbiohub.org/public/igem/BBa_I13607/annotation2228732/range2228732/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I13607/annotation2228732/range2228732/1
type: Range
persistent identity: range2228732
display id: range2228732
version: 1
direction: direction/reverse
top level: BBa_I13607
start: 1051
end: 1967
orientation: inline

BBa_I6061
type: SequenceAnnotation
persistent identity: annotation2228713
display id: annotation2228713
version: 1
top level: BBa_I13607
location: https://synbiohub.org/public/igem/BBa_I13607/annotation2228713/range2228713/1
component: BBa_I6061

https://synbiohub.org/public/igem/BBa_I13607/annotation2228713/range2228713/1
type: Range
persistent identity: range2228713
display id: range2228713
version: 1
direction: direction/reverse
top level: BBa_I13607
start: 1
end: 980
orientation: inline

BBa_K515107
type: ComponentDefinition
persistent identity: BBa K515107
display id: BBa_K515107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Composite p(tetR) - Dendra2
creator: Atipat Patharagulpong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_K515007
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K515007
sequence: https://synbiohub.org/public/igem/BBa_K515107_sequence/1

BBa_R0040
type: Component
persistent identity: component2128589
display id: component2128589
version: 1
top level: BBa_K515107
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K515007
type: Component
persistent identity: component2128597
display id: component2128597
version: 1
top level: BBa_K515107
access: public
definition: https://synbiohub.org/public/igem/BBa_K515007/1

BBa_B0034
type: Component
persistent identity: component2128595
display id: component2128595
version: 1
top level: BBa_K515107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2128589
display id: annotation2128589
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128589/range2128589/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K515107/annotation2128589/range2128589/1
type: Range
persistent identity: range2128589
display id: range2128589
version: 1
direction: direction/forward
top level: BBa_K515107
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128595
display id: annotation2128595
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128595/range2128595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K515107/annotation2128595/range2128595/1
type: Range
persistent identity: range2128595
display id: range2128595
version: 1
direction: direction/reverse
top level: BBa_K515107
start: 63
end: 74
orientation: inline

BBa_K515007
type: SequenceAnnotation
persistent identity: annotation2128597
display id: annotation2128597
version: 1
top level: BBa_K515107
location: https://synbiohub.org/public/igem/BBa_K515107/annotation2128597/range2128597/1
component: BBa_K515007

https://synbiohub.org/public/igem/BBa_K515107/annotation2128597/range2128597/1
type: Range
persistent identity: range2128597
display id: range2128597
version: 1
direction: direction/reverse
top level: BBa_K515107
start: 81
end: 761
orientation: inline

AnRe
type: ComponentDefinition
persistent identity: BBa K772006
display id: BBa_K772006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter/inducer device or protein (enzyme etc.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the inducer/reporter. In conclusion, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Double Terminator
sequence annotation: GFP
sequence annotation: Anchor - Reporter
sequence annotation: TEV cleavage site
sequence annotation: LacZ
sequence: https://synbiohub.org/public/igem/BBa_K772006_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203903
display id: annotation2203903
version: 1
top level: AnRe
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1
type: Range
persistent identity: range2203903
display id: range2203903
version: 1
direction: direction/forward
top level: AnRe
start: 1
end: 20
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203907
display id: annotation2203907
version: 1
top level: AnRe
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1
type: Range
persistent identity: range2203907
display id: range2203907
version: 1
direction: direction/forward
top level: AnRe
start: 1076
end: 1207
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203904
display id: annotation2203904
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1
type: Range
persistent identity: range2203904
display id: range2203904
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 741
orientation: inline

Anchor - Reporter
type: SequenceAnnotation
persistent identity: annotation2203908
display id: annotation2203908
version: 1
top level: AnRe
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1
type: Range
persistent identity: range2203908
display id: range2203908
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 1075
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203905
display id: annotation2203905
version: 1
top level: AnRe
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1
type: Range
persistent identity: range2203905
display id: range2203905
version: 1
direction: direction/forward
top level: AnRe
start: 742
end: 831
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203906
display id: annotation2203906
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1
type: Range
persistent identity: range2203906
display id: range2203906
version: 1
direction: direction/forward
top level: AnRe
start: 832
end: 1075
orientation: inline

BBa_I13605
type: ComponentDefinition
persistent identity: BBa I13605
display id: BBa_I13605
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter Construct (Ly-Tc-) for constitutive CFP and inducible YFP
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter plasmid with Lac operator+YFP(LVA-) reporter followed by a Tet operator+CFP(LVA-) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_E0420
component: BBa_I6031
sequence annotation: BBa_I6031
sequence annotation: BBa_R0040
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_I13605_sequence/1

BBa_R0040
type: Component
persistent identity: component2228641
display id: component2228641
version: 1
top level: BBa_I13605
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0420
type: Component
persistent identity: component2228655
display id: component2228655
version: 1
top level: BBa_I13605
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_I6031
type: Component
persistent identity: component2228640
display id: component2228640
version: 1
top level: BBa_I13605
access: public
definition: https://synbiohub.org/public/igem/BBa_I6031/1

BBa_I6031
type: SequenceAnnotation
persistent identity: annotation2228640
display id: annotation2228640
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228640/range2228640/1
component: BBa_I6031

https://synbiohub.org/public/igem/BBa_I13605/annotation2228640/range2228640/1
type: Range
persistent identity: range2228640
display id: range2228640
version: 1
direction: direction/reverse
top level: BBa_I13605
start: 1
end: 941
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2228641
display id: annotation2228641
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228641/range2228641/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13605/annotation2228641/range2228641/1
type: Range
persistent identity: range2228641
display id: range2228641
version: 1
direction: direction/forward
top level: BBa_I13605
start: 950
end: 1003
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2228655
display id: annotation2228655
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228655/range2228655/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13605/annotation2228655/range2228655/1
type: Range
persistent identity: range2228655
display id: range2228655
version: 1
direction: direction/reverse
top level: BBa_I13605
start: 1012
end: 1889
orientation: inline