//classic/reporter/constitutive
type: Collection
persistent identity: classic reporter constitutive
display id: classic_reporter_constitutive
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1468001
member: BBa_K518012
member: BBa_K993002
member: BBa_K993000
member: BBa_K389302
member: BBa_K1020011
member: BBa_K256003
member: BBa_K837000
member: BBa_K1468002
member: Strong+CFP
member: BBa_K1468000
member: BBa_K879222
member: BBa_K993003
member: BBa_J04430
member: BBa_K389307
member: BBa_K1468004
member: BBa_K822003
member: BBa_K1886005
member: BBa_K389318
member: BBa_I13006
member: BBa_K515105
member: BBa_K546547

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

BBa_K1020011
type: ComponentDefinition
persistent identity: BBa K1020011
display id: BBa_K1020011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High expressed Alkane Selector for intracellular alkane production
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1020007
component: BBa_K1020010
sequence annotation: BBa_K1020007
sequence annotation: BBa_K1020010
sequence: https://synbiohub.org/public/igem/BBa_K1020011_sequence/1

BBa_K1020007
type: Component
persistent identity: component2373785
display id: component2373785
version: 1
top level: BBa_K1020011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020007/1

BBa_K1020010
type: Component
persistent identity: component2373772
display id: component2373772
version: 1
top level: BBa_K1020011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020010/1

BBa_K1020007
type: SequenceAnnotation
persistent identity: annotation2373785
display id: annotation2373785
version: 1
top level: BBa_K1020011
location: https://synbiohub.org/public/igem/BBa_K1020011/annotation2373785/range2373785/1
component: BBa_K1020007

https://synbiohub.org/public/igem/BBa_K1020011/annotation2373785/range2373785/1
type: Range
persistent identity: range2373785
display id: range2373785
version: 1
direction: direction/reverse
top level: BBa_K1020011
start: 1538
end: 2662
orientation: inline

BBa_K1020010
type: SequenceAnnotation
persistent identity: annotation2373772
display id: annotation2373772
version: 1
top level: BBa_K1020011
location: https://synbiohub.org/public/igem/BBa_K1020011/annotation2373772/range2373772/1
component: BBa_K1020010

https://synbiohub.org/public/igem/BBa_K1020011/annotation2373772/range2373772/1
type: Range
persistent identity: range2373772
display id: range2373772
version: 1
direction: direction/reverse
top level: BBa_K1020011
start: 1
end: 1529
orientation: inline

BBa_K546547
type: ComponentDefinition
persistent identity: BBa K546547
display id: BBa_K546547
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive (tetR repressible) LacI and RFP expression
creator: Brendan Ryback, Matthijn Hesselman
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _713_
m user id: 0
owner id: 8616
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part constitutively expresses LacI and RFP except when repressed by TetR. When cells containing this part are exposed to the inducer molecule IPTG, it can be used to regulate lac-repressible promoters. It was designed to function as an IPTG-controllable tuner for the [http://partsregistry.org/Part:BBa_K546546 Double Tunable Synchronized Oscillator]. It is advisable to operate this part on a low or medium copy plasmid.
mutable notes: No specific considerations.
mutable provenance: Composite part of R0040 and J24675
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0040
component: BBa_J24675
sequence annotation: BBa_R0040
sequence annotation: BBa_J24675
sequence: https://synbiohub.org/public/igem/BBa_K546547_sequence/1

BBa_R0040
type: Component
persistent identity: component2268506
display id: component2268506
version: 1
top level: BBa_K546547
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J24675
type: Component
persistent identity: component2268530
display id: component2268530
version: 1
top level: BBa_K546547
access: public
definition: https://synbiohub.org/public/igem/BBa_J24675/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2268506
display id: annotation2268506
version: 1
top level: BBa_K546547
location: https://synbiohub.org/public/igem/BBa_K546547/annotation2268506/range2268506/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K546547/annotation2268506/range2268506/1
type: Range
persistent identity: range2268506
display id: range2268506
version: 1
direction: direction/forward
top level: BBa_K546547
start: 1
end: 54
orientation: inline

BBa_J24675
type: SequenceAnnotation
persistent identity: annotation2268530
display id: annotation2268530
version: 1
top level: BBa_K546547
location: https://synbiohub.org/public/igem/BBa_K546547/annotation2268530/range2268530/1
component: BBa_J24675

https://synbiohub.org/public/igem/BBa_K546547/annotation2268530/range2268530/1
type: Range
persistent identity: range2268530
display id: range2268530
version: 1
direction: direction/reverse
top level: BBa_K546547
start: 63
end: 2110
orientation: inline

BBa_K993000
type: ComponentDefinition
persistent identity: BBa K993000
display id: BBa_K993000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Generator with ROSE thermometer
creator: ABD??LKADİR KARADAĞ
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
group u list: _1290_
m user id: 0
owner id: 11156
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Bradyrhizobium japonicum USDA 110 and Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Start Codon
sequence annotation: RFP Generator
sequence annotation: pVeg2
sequence annotation: Finish Codon
sequence annotation: ROSE Thermometer
sequence: https://synbiohub.org/public/igem/BBa_K993000_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329457
display id: annotation2329457
version: 1
top level: BBa_K993000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1
type: Range
persistent identity: range2329457
display id: range2329457
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 196
orientation: inline

RFP Generator
type: SequenceAnnotation
persistent identity: annotation2329455
display id: annotation2329455
version: 1
top level: BBa_K993000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1
type: Range
persistent identity: range2329455
display id: range2329455
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 902
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329453
display id: annotation2329453
version: 1
top level: BBa_K993000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1
type: Range
persistent identity: range2329453
display id: range2329453
version: 1
direction: direction/forward
top level: BBa_K993000
start: 1
end: 97
orientation: inline

Finish Codon
type: SequenceAnnotation
persistent identity: annotation2329456
display id: annotation2329456
version: 1
top level: BBa_K993000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1
type: Range
persistent identity: range2329456
display id: range2329456
version: 1
direction: direction/forward
top level: BBa_K993000
start: 900
end: 902
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329454
display id: annotation2329454
version: 1
top level: BBa_K993000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1
type: Range
persistent identity: range2329454
display id: range2329454
version: 1
direction: direction/forward
top level: BBa_K993000
start: 98
end: 193
orientation: inline

BBa_K837000
type: ComponentDefinition
persistent identity: BBa K837000
display id: BBa_K837000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNAT.1-eGFP-pSB1C3
creator: Sun Xiaochen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1096_
m user id: 0
owner id: 12280
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part include a RNAT, which is a temperature-sensing sequence, in front of the reporter eGFP. As the environmental temperature changes below 30℃, the RNAT folds into a series of different secondary structures and block ribosomes??? access to the mRNA thus hinder translation of the eGFP.
mutable notes: The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence.
mutable provenance: The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNAT.1
sequence annotation: Preffix
sequence annotation: SD
sequence annotation: Suffix
sequence annotation: EGFP
sequence: https://synbiohub.org/public/igem/BBa_K837000_sequence/1

RNAT.1
type: SequenceAnnotation
persistent identity: annotation2200737
display id: annotation2200737
version: 1
top level: BBa_K837000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K837000/annotation2200737/range2200737/1

https://synbiohub.org/public/igem/BBa_K837000/annotation2200737/range2200737/1
type: Range
persistent identity: range2200737
display id: range2200737
version: 1
direction: direction/forward
top level: BBa_K837000
start: 23
end: 69
orientation: inline

Preffix
type: SequenceAnnotation
persistent identity: annotation2200762
display id: annotation2200762
version: 1
top level: BBa_K837000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K837000/annotation2200762/range2200762/1

https://synbiohub.org/public/igem/BBa_K837000/annotation2200762/range2200762/1
type: Range
persistent identity: range2200762
display id: range2200762
version: 1
direction: direction/forward
top level: BBa_K837000
start: 790
end: 810
orientation: inline

SD
type: SequenceAnnotation
persistent identity: annotation2200735
display id: annotation2200735
version: 1
top level: BBa_K837000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K837000/annotation2200735/range2200735/1

https://synbiohub.org/public/igem/BBa_K837000/annotation2200735/range2200735/1
type: Range
persistent identity: range2200735
display id: range2200735
version: 1
direction: direction/forward
top level: BBa_K837000
start: 55
end: 61
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation2200749
display id: annotation2200749
version: 1
top level: BBa_K837000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K837000/annotation2200749/range2200749/1

https://synbiohub.org/public/igem/BBa_K837000/annotation2200749/range2200749/1
type: Range
persistent identity: range2200749
display id: range2200749
version: 1
direction: direction/forward
top level: BBa_K837000
start: 1
end: 22
orientation: inline

EGFP
type: SequenceAnnotation
persistent identity: annotation2200736
display id: annotation2200736
version: 1
top level: BBa_K837000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K837000/annotation2200736/range2200736/1

https://synbiohub.org/public/igem/BBa_K837000/annotation2200736/range2200736/1
type: Range
persistent identity: range2200736
display id: range2200736
version: 1
direction: direction/forward
top level: BBa_K837000
start: 70
end: 789
orientation: inline

BBa_K518012
type: ComponentDefinition
persistent identity: BBa K518012
display id: BBa_K518012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + RFP + d.Ter
creator: Masato Ohgishi
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an RFP coding device. When ligated with a promoter, it will yield a red colony so that you can determine whether your promoter works.
mutable notes: None.
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0014
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_B0014
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K518012_sequence/1

BBa_B0014
type: Component
persistent identity: component2250787
display id: component2250787
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_E1010
type: Component
persistent identity: component2250780
display id: component2250780
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0032
type: Component
persistent identity: component2250776
display id: component2250776
version: 1
top level: BBa_K518012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250776
display id: annotation2250776
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518012/annotation2250776/range2250776/1
type: Range
persistent identity: range2250776
display id: range2250776
version: 1
direction: direction/forward
top level: BBa_K518012
start: 1
end: 13
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2250787
display id: annotation2250787
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518012/annotation2250787/range2250787/1
type: Range
persistent identity: range2250787
display id: range2250787
version: 1
direction: direction/reverse
top level: BBa_K518012
start: 734
end: 828
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250780
display id: annotation2250780
version: 1
top level: BBa_K518012
location: https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K518012/annotation2250780/range2250780/1
type: Range
persistent identity: range2250780
display id: range2250780
version: 1
direction: direction/reverse
top level: BBa_K518012
start: 20
end: 725
orientation: inline

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_K389302
type: ComponentDefinition
persistent identity: BBa K389302
display id: BBa_K389302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a weak constitutive promoter.
mutable notes: weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389004
component: BBa_J23103
component: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389302_sequence/1

BBa_K389004
type: Component
persistent identity: component2085578
display id: component2085578
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_J23103
type: Component
persistent identity: component2085573
display id: component2085573
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_B0034
type: Component
persistent identity: component2085575
display id: component2085575
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2085573
display id: annotation2085573
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
type: Range
persistent identity: range2085573
display id: range2085573
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085578
display id: annotation2085578
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
type: Range
persistent identity: range2085578
display id: range2085578
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085575
display id: annotation2085575
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
type: Range
persistent identity: range2085575
display id: range2085575
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 44
end: 55
orientation: inline

BBa_K389307
type: ComponentDefinition
persistent identity: BBa K389307
display id: BBa_K389307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a medium strong constitutive promoter.
mutable notes: medium strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23115
component: BBa_B0034
component: BBa_K389004
sequence annotation: BBa_J23115
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389307_sequence/1

BBa_J23115
type: Component
persistent identity: component2085579
display id: component2085579
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_J23115/1

BBa_B0034
type: Component
persistent identity: component2085581
display id: component2085581
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2085584
display id: component2085584
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2085579
display id: annotation2085579
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
type: Range
persistent identity: range2085579
display id: range2085579
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085584
display id: annotation2085584
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
type: Range
persistent identity: range2085584
display id: range2085584
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085581
display id: annotation2085581
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
type: Range
persistent identity: range2085581
display id: range2085581
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 44
end: 55
orientation: inline

BBa_K993003
type: ComponentDefinition
persistent identity: BBa K993003
display id: BBa_K993003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Urease generator with ROSE thermometer
creator: FURKAN SACİT CEYLAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1290_
m user id: 0
owner id: 11155
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGA…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: ROSE Thermometer
sequence annotation: Stop Codon
sequence annotation: Start Codon
sequence annotation: RBS
sequence annotation: pVeg2
sequence annotation: Insulator
sequence annotation: Urease B
sequence annotation: Urease A
sequence annotation: Urease C
sequence: https://synbiohub.org/public/igem/BBa_K993003_sequence/1

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329458
display id: annotation2329458
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1
type: Range
persistent identity: range2329458
display id: range2329458
version: 1
direction: direction/forward
top level: BBa_K993003
start: 98
end: 193
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329470
display id: annotation2329470
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1
type: Range
persistent identity: range2329470
display id: range2329470
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2320
end: 2322
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329468
display id: annotation2329468
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1
type: Range
persistent identity: range2329468
display id: range2329468
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2363
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329465
display id: annotation2329465
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1
type: Range
persistent identity: range2329465
display id: range2329465
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2338
end: 2360
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329447
display id: annotation2329447
version: 1
top level: BBa_K993003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1
type: Range
persistent identity: range2329447
display id: range2329447
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1
end: 97
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329464
display id: annotation2329464
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1
type: Range
persistent identity: range2329464
display id: range2329464
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2323
end: 2337
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329471
display id: annotation2329471
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1
type: Range
persistent identity: range2329471
display id: range2329471
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2661
end: 2663
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329461
display id: annotation2329461
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1
type: Range
persistent identity: range2329461
display id: range2329461
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1919
end: 1941
orientation: inline

Urease B
type: SequenceAnnotation
persistent identity: annotation2329463
display id: annotation2329463
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1
type: Range
persistent identity: range2329463
display id: range2329463
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 2322
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329469
display id: annotation2329469
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1
type: Range
persistent identity: range2329469
display id: range2329469
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1901
end: 1903
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329467
display id: annotation2329467
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1
type: Range
persistent identity: range2329467
display id: range2329467
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 1944
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329462
display id: annotation2329462
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1
type: Range
persistent identity: range2329462
display id: range2329462
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 196
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329460
display id: annotation2329460
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1
type: Range
persistent identity: range2329460
display id: range2329460
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1904
end: 1918
orientation: inline

Urease A
type: SequenceAnnotation
persistent identity: annotation2329459
display id: annotation2329459
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1
type: Range
persistent identity: range2329459
display id: range2329459
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 1903
orientation: inline

Urease C
type: SequenceAnnotation
persistent identity: annotation2329466
display id: annotation2329466
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1
type: Range
persistent identity: range2329466
display id: range2329466
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2663
orientation: inline

BBa_K515105
type: ComponentDefinition
persistent identity: BBa K515105
display id: BBa_K515105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 promoter - sfGFP
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part between J23100 and sfGFP
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K515005
sequence annotation: BBa_K515005
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K515105_sequence/1

BBa_J23100
type: Component
persistent identity: component2219026
display id: component2219026
version: 1
top level: BBa_K515105
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K515005
type: Component
persistent identity: component2219029
display id: component2219029
version: 1
top level: BBa_K515105
access: public
definition: https://synbiohub.org/public/igem/BBa_K515005/1

BBa_K515005
type: SequenceAnnotation
persistent identity: annotation2219029
display id: annotation2219029
version: 1
top level: BBa_K515105
location: https://synbiohub.org/public/igem/BBa_K515105/annotation2219029/range2219029/1
component: BBa_K515005

https://synbiohub.org/public/igem/BBa_K515105/annotation2219029/range2219029/1
type: Range
persistent identity: range2219029
display id: range2219029
version: 1
direction: direction/reverse
top level: BBa_K515105
start: 44
end: 787
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2219026
display id: annotation2219026
version: 1
top level: BBa_K515105
location: https://synbiohub.org/public/igem/BBa_K515105/annotation2219026/range2219026/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515105/annotation2219026/range2219026/1
type: Range
persistent identity: range2219026
display id: range2219026
version: 1
direction: direction/reverse
top level: BBa_K515105
start: 1
end: 35
orientation: inline

BBa_K389318
type: ComponentDefinition
persistent identity: BBa K389318
display id: BBa_K389318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a strong constitutive promoter.
mutable notes: strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23102
component: BBa_B0034
component: BBa_K389004
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence annotation: BBa_J23102
sequence: https://synbiohub.org/public/igem/BBa_K389318_sequence/1

BBa_J23102
type: Component
persistent identity: component2085585
display id: component2085585
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_B0034
type: Component
persistent identity: component2085587
display id: component2085587
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2085590
display id: component2085590
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_K389004/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085590
display id: annotation2085590
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
type: Range
persistent identity: range2085590
display id: range2085590
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085587
display id: annotation2085587
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
type: Range
persistent identity: range2085587
display id: range2085587
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 44
end: 55
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2085585
display id: annotation2085585
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
type: Range
persistent identity: range2085585
display id: range2085585
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 1
end: 35
orientation: inline

BBa_J04430
type: ComponentDefinition
persistent identity: BBa J04430
display id: BBa_J04430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device switched on by IPTG
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _
m user id: 0
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Contains IPTG inducible promoter, an RBS, GFP (no LVA tag), and a terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0010
component: BBa_E0040
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04430_sequence/1

BBa_B0012
type: Component
persistent identity: component1507964
display id: component1507964
version: 1
top level: BBa_J04430
access: public
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BBa_R0010
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display id: component1507937
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0040
type: Component
persistent identity: component1507949
display id: component1507949
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1507954
display id: component1507954
version: 1
top level: BBa_J04430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1507947
display id: component1507947
version: 1
top level: BBa_J04430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1507949
display id: annotation1507949
version: 1
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location: https://synbiohub.org/public/igem/BBa_J04430/annotation1507949/range1507949/1
component: BBa_E0040

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display id: range1507949
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BBa_R0010
type: SequenceAnnotation
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display id: annotation1507937
version: 1
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location: https://synbiohub.org/public/igem/BBa_J04430/annotation1507937/range1507937/1
component: BBa_R0010

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persistent identity: range1507937
display id: range1507937
version: 1
direction: direction/forward
top level: BBa_J04430
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1507954
display id: annotation1507954
version: 1
top level: BBa_J04430
location: https://synbiohub.org/public/igem/BBa_J04430/annotation1507954/range1507954/1
component: BBa_B0010

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display id: range1507954
version: 1
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start: 955
end: 1034
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1507964
display id: annotation1507964
version: 1
top level: BBa_J04430
location: https://synbiohub.org/public/igem/BBa_J04430/annotation1507964/range1507964/1
component: BBa_B0012

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direction: direction/forward
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start: 1043
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1507947
display id: annotation1507947
version: 1
top level: BBa_J04430
location: https://synbiohub.org/public/igem/BBa_J04430/annotation1507947/range1507947/1
component: BBa_B0034

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type: Range
persistent identity: range1507947
display id: range1507947
version: 1
direction: direction/forward
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start: 209
end: 220
orientation: inline

BBa_I13006
type: ComponentDefinition
persistent identity: BBa I13006
display id: BBa_I13006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter R0040 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13006_sequence/1

BBa_B0012
type: Component
persistent identity: component943581
display id: component943581
version: 1
top level: BBa_I13006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component943561
display id: component943561
version: 1
top level: BBa_I13006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component943571
display id: component943571
version: 1
top level: BBa_I13006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component943566
display id: component943566
version: 1
top level: BBa_I13006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: SequenceAnnotation
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display id: annotation943571
version: 1
top level: BBa_I13006
location: https://synbiohub.org/public/igem/BBa_I13006/annotation943571/range943571/1
component: BBa_B0010

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start: 792
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BBa_R0040
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display id: annotation943561
version: 1
top level: BBa_I13006
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component: BBa_R0040

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persistent identity: range943561
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version: 1
direction: direction/forward
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943581
display id: annotation943581
version: 1
top level: BBa_I13006
location: https://synbiohub.org/public/igem/BBa_I13006/annotation943581/range943581/1
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type: Range
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BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943566
display id: annotation943566
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13006/annotation943566/range943566/1
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type: Range
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orientation: inline

Strong+CFP
type: ComponentDefinition
persistent identity: BBa K320005
display id: BBa_K320005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive expression of CFP
creator: Perrudet Christian
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _439_
m user id: 0
owner id: 7149
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sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: CFP
sequence annotation: BBa_K320002
sequence: https://synbiohub.org/public/igem/BBa_K320005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2091399
display id: annotation2091399
version: 1
top level: Strong+CFP
role: feature/binding
role: SO:0001091
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type: Range
persistent identity: range2091399
display id: range2091399
version: 1
direction: direction/forward
top level: Strong+CFP
start: 75
end: 80
orientation: inline

CFP
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persistent identity: annotation2091400
display id: annotation2091400
version: 1
top level: Strong+CFP
role: SO:0000316
role: feature/protein
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type: Range
persistent identity: range2091400
display id: range2091400
version: 1
direction: direction/forward
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end: 168
orientation: inline

BBa_K320002
type: SequenceAnnotation
persistent identity: annotation2088718
display id: annotation2088718
version: 1
top level: Strong+CFP
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2088718
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version: 1
direction: direction/reverse
top level: Strong+CFP
start: 1
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orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0040
sequence annotation: START
sequence annotation: BBa_J23104
sequence annotation: ZsGreeen
sequence annotation: BBa_K1468000
sequence annotation: J23104p
sequence annotation: ZsGreen protein
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

BBa_E0040
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display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2418060
display id: range2418060
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START
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display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
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type: Range
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display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

BBa_J23104
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version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

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type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
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orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

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type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

BBa_K256003
type: ComponentDefinition
persistent identity: BBa K256003
display id: BBa_K256003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP Reporter
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_J23119
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K256003_sequence/1

BBa_E0040
type: Component
persistent identity: component2048137
display id: component2048137
version: 1
top level: BBa_K256003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2048135
display id: component2048135
version: 1
top level: BBa_K256003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23119
type: Component
persistent identity: component2048133
display id: component2048133
version: 1
top level: BBa_K256003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0012
type: Component
persistent identity: component2048140
display id: component2048140
version: 1
top level: BBa_K256003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2048138
display id: component2048138
version: 1
top level: BBa_K256003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2048133
display id: annotation2048133
version: 1
top level: BBa_K256003
location: https://synbiohub.org/public/igem/BBa_K256003/annotation2048133/range2048133/1
component: BBa_J23119

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type: Range
persistent identity: range2048133
display id: range2048133
version: 1
direction: direction/reverse
top level: BBa_K256003
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048140
display id: annotation2048140
version: 1
top level: BBa_K256003
location: https://synbiohub.org/public/igem/BBa_K256003/annotation2048140/range2048140/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256003/annotation2048140/range2048140/1
type: Range
persistent identity: range2048140
display id: range2048140
version: 1
direction: direction/forward
top level: BBa_K256003
start: 878
end: 918
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2048135
display id: annotation2048135
version: 1
top level: BBa_K256003
location: https://synbiohub.org/public/igem/BBa_K256003/annotation2048135/range2048135/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256003/annotation2048135/range2048135/1
type: Range
persistent identity: range2048135
display id: range2048135
version: 1
direction: direction/reverse
top level: BBa_K256003
start: 44
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048138
display id: annotation2048138
version: 1
top level: BBa_K256003
location: https://synbiohub.org/public/igem/BBa_K256003/annotation2048138/range2048138/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256003/annotation2048138/range2048138/1
type: Range
persistent identity: range2048138
display id: range2048138
version: 1
direction: direction/forward
top level: BBa_K256003
start: 790
end: 869
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2048137
display id: annotation2048137
version: 1
top level: BBa_K256003
location: https://synbiohub.org/public/igem/BBa_K256003/annotation2048137/range2048137/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256003/annotation2048137/range2048137/1
type: Range
persistent identity: range2048137
display id: range2048137
version: 1
direction: direction/reverse
top level: BBa_K256003
start: 62
end: 781
orientation: inline

BBa_K1886005
type: ComponentDefinition
persistent identity: BBa K1886005
display id: BBa_K1886005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter-ho1-pcyA
creator: Lejian Jiang
created: 2016-10-05T11:00:00Z
modified: 2016-10-17T01:11:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This circuit is composed of the constitutive promoter, ho1 chromoprotein and pcya chormoprotein. They facilitate Membrane protein ccaS and cph8 to be activated by light of certain wavelength.
mutable notes: Protein ho1 and protein pcyA have to combine with each other to function, so we put them behind the same promoter.
mutable provenance: This part is from paper "Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems", and obtained from iGEM team HZAU's lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I15009
component: BBa_B0015
component: BBa_B0034
component: BBa_I15008
component: BBa_J23108
sequence annotation: BBa_I15009
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_B0015
sequence annotation: BBa_J23108
sequence: https://synbiohub.org/public/igem/BBa_K1886005_sequence/1

BBa_I15009
type: Component
persistent identity: component2486592
display id: component2486592
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0015
type: Component
persistent identity: component2486599
display id: component2486599
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2486589
display id: component2486589
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15008
type: Component
persistent identity: component2486587
display id: component2486587
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_J23108
type: Component
persistent identity: component2486583
display id: component2486583
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23108/1

BBa_B0034
type: Component
persistent identity: component2486585
display id: component2486585
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2486592
display id: annotation2486592
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486592/range2486592/1
component: BBa_I15009

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display id: range2486592
version: 1
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2486589
display id: annotation2486589
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486589/range2486589/1
component: BBa_B0034

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persistent identity: range2486589
display id: range2486589
version: 1
direction: direction/reverse
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end: 832
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2486587
display id: annotation2486587
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486587/range2486587/1
component: BBa_I15008

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persistent identity: range2486587
display id: range2486587
version: 1
direction: direction/reverse
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2486599
display id: annotation2486599
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486599/range2486599/1
component: BBa_B0015

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display id: range2486599
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direction: direction/reverse
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2486585
display id: annotation2486585
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486585/range2486585/1
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persistent identity: range2486585
display id: range2486585
version: 1
direction: direction/reverse
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start: 44
end: 55
orientation: inline

BBa_J23108
type: SequenceAnnotation
persistent identity: annotation2486583
display id: annotation2486583
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486583/range2486583/1
component: BBa_J23108

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type: Range
persistent identity: range2486583
display id: range2486583
version: 1
direction: direction/reverse
top level: BBa_K1886005
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orientation: inline

BBa_K822003
type: ComponentDefinition
persistent identity: BBa K822003
display id: BBa_K822003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive YFP generator
creator: Jarle Pahr
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1080_
m user id: 0
owner id: 11627
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments.
mutable notes: N/A
mutable provenance: Listed BioBrick parts from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_E0030
component: BBa_K081005
sequence annotation: BBa_B0015
sequence annotation: BBa_E0030
sequence annotation: BBa_K081005
sequence: https://synbiohub.org/public/igem/BBa_K822003_sequence/1

BBa_B0015
type: Component
persistent identity: component2197106
display id: component2197106
version: 1
top level: BBa_K822003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0030
type: Component
persistent identity: component2197099
display id: component2197099
version: 1
top level: BBa_K822003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_K081005
type: Component
persistent identity: component2197098
display id: component2197098
version: 1
top level: BBa_K822003
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2197106
display id: annotation2197106
version: 1
top level: BBa_K822003
location: https://synbiohub.org/public/igem/BBa_K822003/annotation2197106/range2197106/1
component: BBa_B0015

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persistent identity: range2197106
display id: range2197106
version: 1
direction: direction/reverse
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start: 796
end: 924
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2197099
display id: annotation2197099
version: 1
top level: BBa_K822003
location: https://synbiohub.org/public/igem/BBa_K822003/annotation2197099/range2197099/1
component: BBa_E0030

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type: Range
persistent identity: range2197099
display id: range2197099
version: 1
direction: direction/reverse
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start: 65
end: 787
orientation: inline

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2197098
display id: annotation2197098
version: 1
top level: BBa_K822003
location: https://synbiohub.org/public/igem/BBa_K822003/annotation2197098/range2197098/1
component: BBa_K081005

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type: Range
persistent identity: range2197098
display id: range2197098
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_K879222
type: ComponentDefinition
persistent identity: BBa K879222
display id: BBa_K879222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue with promoter 2
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Blue with promoter 2
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879092
component: BBa_K879022
component: BBa_K879032
component: BBa_K879091
component: BBa_K879012
sequence annotation: BBa_K879022
sequence annotation: BBa_K879012
sequence annotation: BBa_K879091
sequence annotation: BBa_K879092
sequence annotation: BBa_K879032
sequence: https://synbiohub.org/public/igem/BBa_K879222_sequence/1

BBa_K879092
type: Component
persistent identity: component2205048
display id: component2205048
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879022
type: Component
persistent identity: component2205049
display id: component2205049
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879022/1

BBa_K879032
type: Component
persistent identity: component2205047
display id: component2205047
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879032/1

BBa_K879091
type: Component
persistent identity: component2205046
display id: component2205046
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879012
type: Component
persistent identity: component2205050
display id: component2205050
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879012/1

BBa_K879022
type: SequenceAnnotation
persistent identity: annotation2205049
display id: annotation2205049
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205049/range2205049/1
component: BBa_K879022

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type: Range
persistent identity: range2205049
display id: range2205049
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 75
end: 103
orientation: inline

BBa_K879012
type: SequenceAnnotation
persistent identity: annotation2205050
display id: annotation2205050
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205050/range2205050/1
component: BBa_K879012

https://synbiohub.org/public/igem/BBa_K879222/annotation2205050/range2205050/1
type: Range
persistent identity: range2205050
display id: range2205050
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 104
end: 772
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205046
display id: annotation2205046
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205046/range2205046/1
component: BBa_K879091

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type: Range
persistent identity: range2205046
display id: range2205046
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 1
end: 36
orientation: inline

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205048
display id: annotation2205048
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205048/range2205048/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879222/annotation2205048/range2205048/1
type: Range
persistent identity: range2205048
display id: range2205048
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 66
end: 74
orientation: inline

BBa_K879032
type: SequenceAnnotation
persistent identity: annotation2205047
display id: annotation2205047
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205047/range2205047/1
component: BBa_K879032

https://synbiohub.org/public/igem/BBa_K879222/annotation2205047/range2205047/1
type: Range
persistent identity: range2205047
display id: range2205047
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 37
end: 65
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K1468004
sequence annotation: STOP
sequence annotation: LacZ protein
sequence annotation: TetR 2
sequence annotation: LacZ
sequence annotation: -10
sequence annotation: BBa_I732005
sequence annotation: -35
sequence annotation: BBa_R0040
sequence annotation: START
sequence annotation: C --> T
sequence annotation: TetR 1
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

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type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

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type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

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type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

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type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

BBa_K993002
type: ComponentDefinition
persistent identity: BBa K993002
display id: BBa_K993002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IbpB RNA Thermometer with RFP
creator: İBRAHİM YASİR ORHAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1290_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli. IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: IbpB thermometer
sequence annotation: Start codon
sequence annotation: RFP
sequence annotation: Stop codon
sequence annotation: pVeg2
sequence: https://synbiohub.org/public/igem/BBa_K993002_sequence/1

IbpB thermometer
type: SequenceAnnotation
persistent identity: annotation2329443
display id: annotation2329443
version: 1
top level: BBa_K993002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1
type: Range
persistent identity: range2329443
display id: range2329443
version: 1
direction: direction/forward
top level: BBa_K993002
start: 98
end: 208
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2329446
display id: annotation2329446
version: 1
top level: BBa_K993002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1
type: Range
persistent identity: range2329446
display id: range2329446
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 211
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2329444
display id: annotation2329444
version: 1
top level: BBa_K993002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1
type: Range
persistent identity: range2329444
display id: range2329444
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 917
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2329445
display id: annotation2329445
version: 1
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pVeg2
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