//classic/rbs
type: Collection
persistent identity: classic rbs
display id: classic_rbs
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //classic/rbs/uncategorized

//classic/rbs/uncategorized
type: Collection
persistent identity: classic rbs uncategorized
display id: classic_rbs_uncategorized
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1471011
member: BBa_I11101
member: BBa_K621001
member: BBa_Q0001

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: C2 OmpR
sequence annotation: C1 OmpR
sequence annotation: -35
sequence annotation: C3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_Q0001
type: ComponentDefinition
persistent identity: BBa Q0001
display id: BBa_Q0001
version: 1
was generated by: iGEM to SBOL conversion
description: Quad Part
created: 2003-09-29T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
discontinued: true
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: 6
type: DnaRegion
role: SO:0000139
role: partType/RBS
component: BBa_B0034
component: BBa_R0050
component: BBa_B0012
component: BBa_B0011
component: BBa_C0050
sequence annotation: BBa_C0050
sequence annotation: BBa_R0050
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_Q0001_sequence/1

BBa_B0034
type: Component
persistent identity: component951505
display id: component951505
version: 1
top level: BBa_Q0001
access: public
definition: BBa_B0034

BBa_R0050
type: Component
persistent identity: component951554
display id: component951554
version: 1
top level: BBa_Q0001
access: public
definition: cI HK022

BBa_B0012
type: Component
persistent identity: component951532
display id: component951532
version: 1
top level: BBa_Q0001
access: public
definition: BBa_B0012

BBa_B0011
type: Component
persistent identity: component951540
display id: component951540
version: 1
top level: BBa_Q0001
access: public
definition: BBa_B0011

BBa_C0050
type: Component
persistent identity: component951520
display id: component951520
version: 1
top level: BBa_Q0001
access: public
definition: cI HK022

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation951520
display id: annotation951520
version: 1
top level: BBa_Q0001
location: https://synbiohub.org/public/igem/BBa_Q0001/annotation951520/range951520/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_Q0001/annotation951520/range951520/1
type: Range
persistent identity: range951520
display id: range951520
version: 1
top level: BBa_Q0001
direction: direction/forward
start: 19
end: 762
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation951554
display id: annotation951554
version: 1
top level: BBa_Q0001
location: https://synbiohub.org/public/igem/BBa_Q0001/annotation951554/range951554/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_Q0001/annotation951554/range951554/1
type: Range
persistent identity: range951554
display id: range951554
version: 1
top level: BBa_Q0001
direction: direction/forward
start: 899
end: 953
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation951532
display id: annotation951532
version: 1
top level: BBa_Q0001
location: https://synbiohub.org/public/igem/BBa_Q0001/annotation951532/range951532/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q0001/annotation951532/range951532/1
type: Range
persistent identity: range951532
display id: range951532
version: 1
top level: BBa_Q0001
direction: direction/forward
start: 796
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation951505
display id: annotation951505
version: 1
top level: BBa_Q0001
location: https://synbiohub.org/public/igem/BBa_Q0001/annotation951505/range951505/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q0001/annotation951505/range951505/1
type: Range
persistent identity: range951505
display id: range951505
version: 1
top level: BBa_Q0001
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation951540
display id: annotation951540
version: 1
top level: BBa_Q0001
location: https://synbiohub.org/public/igem/BBa_Q0001/annotation951540/range951540/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_Q0001/annotation951540/range951540/1
type: Range
persistent identity: range951540
display id: range951540
version: 1
top level: BBa_Q0001
direction: direction/forward
start: 845
end: 890
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: T7 TE
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (HK022 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Derived from <genbank>STHK022N</genbank>. Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR2
sequence annotation: BBa_R0050
sequence annotation: -10
sequence annotation: putative
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
top level: cI HK022
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
top level: cI HK022
direction: direction/forward
start: 13
end: 27
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 55
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
top level: cI HK022
direction: direction/forward
start: 30
end: 35
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
top level: cI HK022
direction: direction/forward
start: 43
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
top level: cI HK022
direction: direction/forward
start: 37
end: 51
orientation: inline

BBa_K1471011
type: ComponentDefinition
persistent identity: BBa K1471011
display id: BBa_K1471011
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Arabidopsis Thaliana).
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:39Z
mutable provenance: Arabidopsis thaliana genome sequence.
owned by: james
owned by: myers
mutable description: The initiation of protein biosynthesis is a major determinant of the efficiency of gene expression at the translational level. It is known that the nucleotide sequences around the AUG translation initiation codon act as an important signal to trigger the initiation of the translation event. (Kozak, 1987)
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 0
owner id: 16464
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury.
star: false
creator: Juan Noe Hernandez Salazar
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Ribosome binds to 5' end.
sequence annotation: Kozak Sequence.
sequence annotation: Receptor codon.
sequence: https://synbiohub.org/public/igem/BBa_K1471011_sequence/1

Ribosome binds to 5' end.
type: SequenceAnnotation
persistent identity: annotation2419569
display id: annotation2419569
version: 1
top level: BBa_K1471011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1471011/annotation2419569/range2419569/1

https://synbiohub.org/public/igem/BBa_K1471011/annotation2419569/range2419569/1
type: Range
persistent identity: range2419569
display id: range2419569
version: 1
top level: BBa_K1471011
direction: direction/forward
start: 1
end: 1
orientation: inline

Kozak Sequence.
type: SequenceAnnotation
persistent identity: annotation2419434
display id: annotation2419434
version: 1
top level: BBa_K1471011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1471011/annotation2419434/range2419434/1

https://synbiohub.org/public/igem/BBa_K1471011/annotation2419434/range2419434/1
type: Range
persistent identity: range2419434
display id: range2419434
version: 1
top level: BBa_K1471011
direction: direction/forward
start: 1
end: 11
orientation: inline

Receptor codon.
type: SequenceAnnotation
persistent identity: annotation2419570
display id: annotation2419570
version: 1
top level: BBa_K1471011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1471011/annotation2419570/range2419570/1

https://synbiohub.org/public/igem/BBa_K1471011/annotation2419570/range2419570/1
type: Range
persistent identity: range2419570
display id: range2419570
version: 1
top level: BBa_K1471011
direction: direction/forward
start: 8
end: 10
orientation: inline

BBa_K621001
type: ComponentDefinition
persistent identity: BBa K621001
display id: BBa_K621001
version: 1
was generated by: iGEM to SBOL conversion
description: OMP-R-->RBS-->eYFP
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:55Z
mutable provenance: This BioBrick was created from parts found in the Registry. These parts were BBa_R0082, BBa_B0032, and BBa_E0030.
owned by: james
owned by: myers
mutable description: Our part consists of an OMP-R binding region that regulates our reporter gene, eYFP. The OMP-R binding region is an omp-c operator, which has three OMP-R binding sites. Our part is regulated by the ENVZ-OMPR two-component regulatory system. ENVZ is a osmolarity sensor on the inner membrane and phosphorylates OMP-R in the presence of high osmolarity. When phosphorylated, OMP-R binds to the omp-c operator and recruits RNAP to transcribe the downstream reporter gene. Fluorescence intensity due to the production of eYFP is a measure of the system's activity. When all three binding sites are occupied by phosphorylated OMP-R proteins, RNAP cannot bind to the DNA and transcribe downstream. This part can be used as an osmolarity indicator. Our team used this part as a glucose sensor because the presence of glucose changes osmolarity. Different concentrations of glucose produce different, quantifiable fluorescence intensities.
discontinued: false
dominant: false
experience: experience/Works
group u list: _793_
m user id: 0
owner id: 4838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Our team had to combine the genes in a specific order. First our team cut out and purified the eYPF gene. We added this gene to a plasmid containing RBS. Next we cut out the RBS+eYFP intermediate and ligated them into the plasmid containing OMP-R. The RBS and OMP-R parts were too small to run out and extract on a gel because they would run off of the end of the gel. Therefore, we had to start the process with eYPF.
star: false
creator: David Pohlman, Blythe Ferriere, Aile Able, Erica McFarland
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_R0082
component: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_R0082
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K621001_sequence/1

BBa_E0030
type: Component
persistent identity: component2145049
display id: component2145049
version: 1
top level: BBa_K621001
access: public
definition: eyfp

BBa_R0082
type: Component
persistent identity: component2145045
display id: component2145045
version: 1
top level: BBa_K621001
access: public
definition: OmpR

BBa_B0032
type: Component
persistent identity: component2145047
display id: component2145047
version: 1
top level: BBa_K621001
access: public
definition: BBa_B0032

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2145049
display id: annotation2145049
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
type: Range
persistent identity: range2145049
display id: range2145049
version: 1
top level: BBa_K621001
direction: direction/reverse
start: 136
end: 858
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2145045
display id: annotation2145045
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
type: Range
persistent identity: range2145045
display id: range2145045
version: 1
top level: BBa_K621001
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2145047
display id: annotation2145047
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
type: Range
persistent identity: range2145047
display id: range2145047
version: 1
top level: BBa_K621001
direction: direction/forward
start: 117
end: 129
orientation: inline

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: ribosome binding site
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: bu
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
top level: BBa_I11010
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_I11101
type: ComponentDefinition
persistent identity: BBa I11101
display id: BBa_I11101
version: 1
was generated by: iGEM to SBOL conversion
description: RBS with KpnI
created: 2004-09-06T11:00:00Z
modified: 2015-08-31T04:07:30Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: bu
type: DnaRegion
role: SO:0000139
role: partType/RBS
component: BBa_I11010
sequence annotation: BBa_I11010
sequence: https://synbiohub.org/public/igem/BBa_I11101_sequence/1

BBa_I11010
type: Component
persistent identity: component1136956
display id: component1136956
version: 1
top level: BBa_I11101
access: public
definition: BBa_I11010

BBa_I11010
type: Component
persistent identity: component1136958
display id: component1136958
version: 1
top level: BBa_I11101
access: public
definition: BBa_I11010

BBa_I11010
type: SequenceAnnotation
persistent identity: annotation1136956
display id: annotation1136956
version: 1
top level: BBa_I11101
location: https://synbiohub.org/public/igem/BBa_I11101/annotation1136956/range1136956/1
component: BBa_I11010

https://synbiohub.org/public/igem/BBa_I11101/annotation1136956/range1136956/1
type: Range
persistent identity: range1136956
display id: range1136956
version: 1
top level: BBa_I11101
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_I11010
type: SequenceAnnotation
persistent identity: annotation1136958
display id: annotation1136958
version: 1
top level: BBa_I11101
location: https://synbiohub.org/public/igem/BBa_I11101/annotation1136958/range1136958/1
component: BBa_I11010

https://synbiohub.org/public/igem/BBa_I11101/annotation1136958/range1136958/1
type: Range
persistent identity: range1136958
display id: range1136958
version: 1
top level: BBa_I11101
direction: direction/forward
start: 15
end: 20
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage HK022 (+LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from <genbank>STHK022N</genbank>. Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It look…
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: OR3 partial
sequence annotation: 2
sequence annotation: BBa_C0050
sequence annotation: SsrA
sequence annotation: cI HK022
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 8
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
top level: cI HK022
direction: direction/forward
start: 739
end: 744
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
top level: cI HK022
direction: direction/forward
start: 706
end: 738
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
top level: cI HK022
direction: direction/forward
start: 745
end: 769
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11010_sequence/1
type: Sequence
persistent identity: BBa I11010 sequence
display id: BBa_I11010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggtcatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q0001_sequence/1
type: Sequence
persistent identity: BBa Q0001 sequence
display id: BBa_Q0001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0050_sequence/1
type: Sequence
persistent identity: BBa C0050 sequence
display id: BBa_C0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1471011_sequence/1
type: Sequence
persistent identity: BBa K1471011 sequence
display id: BBa_K1471011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcaatggcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K621001_sequence/1
type: Sequence
persistent identity: BBa K621001 sequence
display id: BBa_K621001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0050_sequence/1
type: Sequence
persistent identity: BBa R0050 sequence
display id: BBa_R0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11101_sequence/1
type: Sequence
persistent identity: BBa I11101 sequence
display id: BBa_I11101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggtactagagaggagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z