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display id: BBa_I8022
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator test: Inverter NOR biasing in monocistronic design
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the third test chassis for the Booperator and is particular to the monocistronic design. It tests the biasing of the Inverter NOR pair.
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR and LasR constitutive generation (using TetR)
creator: Ronny Krashinsky
created: 2004-01-30T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Produces RhlR and LasR constitutively under control of a TetR self-repressor.
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owned by: myers
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BBa_I2025
type: ComponentDefinition
persistent identity: BBa I2025
display id: BBa_I2025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR Receiver Test (R1075.B0034.C0079.B0015.R0079.E0022)
creator: Sara Neves, Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lasR receiver test: -to test basal activity, give no input of chemical signal, and reporter indicates TIPs leakage -to test binding, put in solution of chemical signal made by lasI (AI-1), the inducer binds to the lasR protein to activate the promoter R0079, to make TIPs that will express reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0079
component: BBa_R1075
component: BBa_B0034
component: BBa_B0010
component: BBa_E0022
component: BBa_R0079
component: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_C0079
sequence annotation: BBa_B0010
sequence annotation: BBa_R1075
sequence annotation: BBa_R0079
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I2025_sequence/1

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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation959055
display id: annotation959055
version: 1
top level: BBa_I2025
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component: BBa_B0034

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type: Range
persistent identity: range959055
display id: range959055
version: 1
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start: 58
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BBa_I8035
type: ComponentDefinition
persistent identity: BBa I8035
display id: BBa_I8035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator aiia test 6: Effect of tetR on production of LuxR
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0012
component: BBa_R0062
component: BBa_B0034
component: BBa_E0032
component: BBa_R0040
component: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I8035_sequence/1

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display id: component968808
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: component968871
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

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display id: component968785
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: component968841
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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persistent identity: component968818
display id: component968818
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

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display id: component968777
version: 1
top level: BBa_I8035
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

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persistent identity: component968861
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version: 1
top level: BBa_I8035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_C0062/1

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version: 1
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display id: range968818
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direction: direction/forward
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display id: annotation968785
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display id: range968800
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persistent identity: annotation968808
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version: 1
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persistent identity: range968808
display id: range968808
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direction: direction/forward
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end: 949
orientation: inline

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type: SequenceAnnotation
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display id: annotation968871
version: 1
top level: BBa_I8035
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display id: range968871
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direction: direction/forward
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persistent identity: range968861
display id: range968861
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version: 1
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display id: range968853
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type: SequenceAnnotation
persistent identity: annotation968841
display id: annotation968841
version: 1
top level: BBa_I8035
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type: Range
persistent identity: range968841
display id: range968841
version: 1
direction: direction/forward
top level: BBa_I8035
start: 1070
end: 1081
orientation: inline

BBa_I13976
type: ComponentDefinition
persistent identity: BBa I13976
display id: BBa_I13976
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac relaxation oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate for lac relaxation oscillator (final stage)
mutable notes: There is a single base insertion (of an A) at position 286, this is in the mixed site between R0011 and B0034 (first subpart of E0034) so suspect it should have little effect on device performance. But no experimental confirmation.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0434
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_E0434
sequence: https://synbiohub.org/public/igem/BBa_I13976_sequence/1

BBa_E0434
type: Component
persistent identity: component2221666
display id: component2221666
version: 1
top level: BBa_I13976
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

BBa_R0011
type: Component
persistent identity: component2221652
display id: component2221652
version: 1
top level: BBa_I13976
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

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type: SequenceAnnotation
persistent identity: annotation2221652
display id: annotation2221652
version: 1
top level: BBa_I13976
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persistent identity: range2221652
display id: range2221652
version: 1
direction: direction/forward
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start: 1
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2221666
display id: annotation2221666
version: 1
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component: BBa_E0434

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display id: range2221666
version: 1
direction: direction/reverse
top level: BBa_I13976
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end: 980
orientation: inline

BBa_J13010
type: ComponentDefinition
persistent identity: BBa J13010
display id: BBa_J13010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI repressed Cph8
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: AspA attractant sender (I3100.I3200.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Lac trigger ability to produce AspA. After trigger induction, the amount of aspartate secreted into the medium can be measured directly.
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owned by: myers
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display id: BBa_I2024
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasI Sender Test (I0500.B0034.C0078.B0015)
creator: Sara Neves, Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lasI test: with input of TIPs from I0500, determines how much chemical signal is ultimately made by the lasI protein amount of signal can be determined chemically (?)
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display id: BBa_J06955
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (Lambda II) RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with lambda cI857 (Lambda II) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
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description: Test: Quorum threshold level (I3510.E0033.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
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mutable description: Tests threshold level of HSL concentration needed to induce the switch quorum response.
mutable notes: This testbed is designed to respond to a manual input of Lux HSL. The output is through the LacZ reporter.
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description: QPI Test Construct Intermediate (Q01400.E0430)
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modified: 2015-08-31T04:07:32Z
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description: Prakash *: Synchronator Design 4
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:41Z
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description: Lambda cI switch output device (R0050.B0034.C0051)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
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status: status/Unavailable
bookmark: false
mutable description: One output from the Hk022cI-lambda cI wired-nor bistable switch.
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owned by: myers
star: false
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description: Hk022cI switch input device (B0034.C0050.B0015)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
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group u list: _1_
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owned by: myers
star: false
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type: ComponentDefinition
persistent identity: BBa I3420
display id: BBa_I3420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests input levels needed to trigger switch, bistability, noise resistance, and switch time. Switch has three independently controllable trigger inputs and two reporter outputs, ON and OFF.
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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star: false
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persistent identity: BBa I3000
display id: BBa_I3000
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 1, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3400.I3300.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 1 has the OR gate embedded within the switch via a double instance of I3400 Hk022cI switch input device. Design 1 also has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
mutable notes: The layout of E. colibrator (I3000) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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BBa_I12028
type: ComponentDefinition
persistent identity: BBa I12028
display id: BBa_I12028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for I12006
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expression of lambda cI controlled by IPTG (requires LacIq strain). Expression of 434 cI controlled by arabinose. If I12006 promoter works, YFP is activated by lambda cI and repressed by 434 cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12024
component: BBa_I12027
component: BBa_E0430
component: BBa_I12006
sequence annotation: BBa_E0430
sequence annotation: BBa_I12027
sequence annotation: BBa_I12024
sequence annotation: BBa_I12006
sequence: https://synbiohub.org/public/igem/BBa_I12028_sequence/1

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access: public
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access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

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access: public
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description: Synchronized Relaxation Oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
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sample status: It's complicated
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bookmark: false
mutable description: This is a potential synchronized relaxation oscillator simmilar to the synchronization element of I13905
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owned by: myers
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display id: BBa_I4204
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash *: Syncronator Cell-Signalling Module
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
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group u list: _1_
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sample status: It's complicated
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BBa_J06954
type: ComponentDefinition
persistent identity: BBa J06954
display id: BBa_J06954
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (E07700) RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with lambda cI (E07700) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
mutable notes: Cancelled due to failure to successfully mutate BBa_J06502 and BBa_J06802.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_R0052
component: BBa_C0052
component: BBa_B0010
component: BBa_B0012
component: BBa_E2060
component: BBa_R0051
component: BBa_J06502
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_R0052
sequence annotation: BBa_J06502
sequence annotation: BBa_B0010
sequence annotation: BBa_E2060
sequence annotation: BBa_C0052
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BBa_B0010
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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type: ComponentDefinition
persistent identity: BBa I2021
display id: BBa_I2021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter II Test (R1075.E0422)
creator: Alvin Carter Powers, Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tests the strength of R1075, indicated by reporter expression
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R1075
component: BBa_E0022
component: BBa_B0034
component: BBa_B0010
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sequence annotation: BBa_E0022
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sequence annotation: BBa_B0010
sequence annotation: BBa_R1075
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BBa_R1075
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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type: ComponentDefinition
persistent identity: BBa I3421
display id: BBa_I3421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3300.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3300 ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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star: false
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BBa_B0012
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BBa_B0010
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BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_C0050/1

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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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BBa_R1075
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BBa_R1075
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definition: https://synbiohub.org/public/igem/BBa_R1075/1

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start: 853
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description: Booperator aiia test: degradation of HSL "A" by aiia
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exogenous LuxI Relaxation Oscillator Test Construct Intermediate (R0062.C0260.E0424)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
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sample status: Not in stock
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description: Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _20_
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RS flip-flop with LacI ts (mut 265) as the set and lambda cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
mutable notes: Sequenced. However, shows a 23 bp deletion in the LacI+pL regulated promoter (R0011).
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display id: BBa_J13202
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL + LuxR reporter and aiia (LVA+) tuning device
creator: Jeffrey J Tabor
created: 2005-09-19T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device uses the S01656 module to encode IPTG (or lactose) inducible aiia (LVA+), allowing the tuning of 3OC6HSL degradation activity in lactose tune-able E.coli strains. The presence of the 3OC6HSL and the LuxR protein is read out by the I6042 module.
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owned by: myers
star: false
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component: BBa_B0010
component: BBa_C0060
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component: BBa_B0012
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component: BBa_R0011
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sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_R0062
sequence annotation: BBa_E0030
sequence annotation: BBa_C0060
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type: ComponentDefinition
persistent identity: BBa I3422
display id: BBa_I3422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3301.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3301 truncated ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3301 truncated upstream ompC version) controls the other (I3301.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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owned by: myers
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BBa_B0012
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BBa_B0034
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BBa_B0034
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BBa_B0034
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BBa_B0012
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BBa_R0083
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BBa_R0051
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

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BBa_C0051
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BBa_C0012
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definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R1075
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display id: component1008291
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definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_C0050
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top level: BBa_I3422
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display id: annotation1008376
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BBa_I15001
type: ComponentDefinition
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display id: BBa_I15001
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI regulated cI/LuxI with constitutive LuxR
creator: jtabor
created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Polycistronic lambda cI and LuxI proteins regulated by Lac Repressor (LacI). Downstream is consitutive LuxR expression cassette. System flanked by dual transcription terminators.
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_C0062
component: BBa_B0034
component: BBa_B0012
component: BBa_R1075
component: BBa_C0061
component: BBa_I1020
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_R1075
sequence annotation: BBa_I1020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_I15001_sequence/1

BBa_B0010
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BBa_C0062
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definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
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display id: component974909
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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display id: component974893
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BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R1075
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definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_C0061
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definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0012
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persistent identity: component974942
display id: component974942
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top level: BBa_I15001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I1020
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display id: component974847
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definition: https://synbiohub.org/public/igem/BBa_I1020/1

BBa_B0010
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display id: component974932
version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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orientation: inline

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component: BBa_C0062

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end: 2617
orientation: inline

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component: BBa_R1075

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orientation: inline

BBa_I1020
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persistent identity: annotation974847
display id: annotation974847
version: 1
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location: https://synbiohub.org/public/igem/BBa_I15001/annotation974847/range974847/1
component: BBa_I1020

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direction: direction/forward
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BBa_B0010
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component: BBa_B0010

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component: BBa_B0012

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persistent identity: range974810
display id: range974810
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orientation: inline

BBa_B0012
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version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974893/range974893/1
component: BBa_B0012

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display id: range974893
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persistent identity: annotation974883
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version: 1
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component: BBa_B0010

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persistent identity: range974883
display id: range974883
version: 1
direction: direction/forward
top level: BBa_I15001
start: 1650
end: 1729
orientation: inline

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persistent identity: annotation974932
display id: annotation974932
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974932/range974932/1
component: BBa_B0010

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persistent identity: range974932
display id: range974932
version: 1
direction: direction/forward
top level: BBa_I15001
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orientation: inline

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version: 1
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component: BBa_B0012

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persistent identity: range974942
display id: range974942
version: 1
direction: direction/forward
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start: 2739
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orientation: inline

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display id: annotation974876
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974876/range974876/1
component: BBa_C0061

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type: Range
persistent identity: range974876
display id: range974876
version: 1
direction: direction/forward
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type: SequenceAnnotation
persistent identity: annotation974909
display id: annotation974909
version: 1
top level: BBa_I15001
location: https://synbiohub.org/public/igem/BBa_I15001/annotation974909/range974909/1
component: BBa_B0034

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type: Range
persistent identity: range974909
display id: range974909
version: 1
direction: direction/forward
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BBa_I12042
type: ComponentDefinition
persistent identity: BBa I12042
display id: BBa_I12042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator, no cooperativity, reporter protein, kickstart
creator: Hans
created: 2004-08-19T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a Barkai-Leibler oscillator with no cooperativity for the repressor (one 434 cI OR site). A reporter protein (EYFP) and a kickstart is included.
mutable notes: There is no cooperativity for 434 cI in this oscillator. A protein EYFP (with degradation tag) coding region is placed directly after the lambda cI coding region in order to keep track of the lambda cI levels. This part also includes a "kickstart" that will yield lambda cI if ATC is added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12043
component: BBa_I12002
component: BBa_I12038
sequence annotation: BBa_I12002
sequence annotation: BBa_I12043
sequence annotation: BBa_I12038
sequence: https://synbiohub.org/public/igem/BBa_I12042_sequence/1

BBa_I12043
type: Component
persistent identity: component2238925
display id: component2238925
version: 1
top level: BBa_I12042
access: public
definition: https://synbiohub.org/public/igem/BBa_I12043/1

BBa_I12002
type: Component
persistent identity: component2238946
display id: component2238946
version: 1
top level: BBa_I12042
access: public
definition: https://synbiohub.org/public/igem/BBa_I12002/1

BBa_I12038
type: Component
persistent identity: component2238886
display id: component2238886
version: 1
top level: BBa_I12042
access: public
definition: BBa_I12038

BBa_I12002
type: SequenceAnnotation
persistent identity: annotation2238946
display id: annotation2238946
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238946/range2238946/1
component: BBa_I12002

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type: Range
persistent identity: range2238946
display id: range2238946
version: 1
direction: direction/reverse
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start: 3111
end: 4128
orientation: inline

BBa_I12043
type: SequenceAnnotation
persistent identity: annotation2238925
display id: annotation2238925
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238925/range2238925/1
component: BBa_I12043

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type: Range
persistent identity: range2238925
display id: range2238925
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 1001
end: 3102
orientation: inline

BBa_I12038
type: SequenceAnnotation
persistent identity: annotation2238886
display id: annotation2238886
version: 1
top level: BBa_I12042
location: https://synbiohub.org/public/igem/BBa_I12042/annotation2238886/range2238886/1
component: BBa_I12038

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type: Range
persistent identity: range2238886
display id: range2238886
version: 1
direction: direction/reverse
top level: BBa_I12042
start: 1
end: 992
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt QPI Intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0072
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_C0072/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

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type: Range
persistent identity: range939411
display id: range939411
version: 1
direction: direction/forward
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start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

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type: Range
persistent identity: range939424
display id: range939424
version: 1
direction: direction/forward
top level: BBa_I13025
start: 19
end: 306
orientation: inline

BBa_I9128
type: ComponentDefinition
persistent identity: BBa I9128
display id: BBa_I9128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (cinRI system) R0077.I9045.10500.E0422.I0500.I9031.I9028
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R0077
component: BBa_E0022
component: BBa_B0034
component: BBa_I0500
component: BBa_E0032
component: BBa_R1075
component: BBa_C0076
component: BBa_C0077
component: BBa_B0012
sequence annotation: BBa_R0077
sequence annotation: BBa_B0010
sequence annotation: BBa_E0022
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0076
sequence annotation: BBa_R1075
sequence annotation: BBa_C0077
sequence annotation: BBa_I0500
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I9128_sequence/1

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persistent identity: component988623
display id: component988623
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0077
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display id: component988603
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

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display id: component988699
version: 1
top level: BBa_I9128
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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persistent identity: component988655
display id: component988655
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0034
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persistent identity: component988683
display id: component988683
version: 1
top level: BBa_I9128
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component988638
version: 1
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definition: https://synbiohub.org/public/igem/BBa_I0500/1

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persistent identity: component988615
display id: component988615
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
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persistent identity: component988643
display id: component988643
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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persistent identity: component988663
display id: component988663
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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type: Component
persistent identity: component988720
display id: component988720
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_R1075/1

BBa_C0076
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persistent identity: component988693
display id: component988693
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_C0076/1

BBa_B0010
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persistent identity: component988741
display id: component988741
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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persistent identity: component988725
display id: component988725
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top level: BBa_I9128
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
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persistent identity: component988678
display id: component988678
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

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persistent identity: component988735
display id: component988735
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0077/1

BBa_B0012
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persistent identity: component988751
display id: component988751
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component988633
display id: component988633
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component988673
display id: component988673
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component988709
display id: component988709
version: 1
top level: BBa_I9128
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation988603
display id: annotation988603
version: 1
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location: https://synbiohub.org/public/igem/BBa_I9128/annotation988603/range988603/1
component: BBa_R0077

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type: Range
persistent identity: range988603
display id: range988603
version: 1
direction: direction/forward
top level: BBa_I9128
start: 1
end: 231
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation988741
display id: annotation988741
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988741/range988741/1
component: BBa_B0010

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type: Range
persistent identity: range988741
display id: range988741
version: 1
direction: direction/forward
top level: BBa_I9128
start: 6266
end: 6345
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation988655
display id: annotation988655
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988655/range988655/1
component: BBa_E0022

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type: Range
persistent identity: range988655
display id: range988655
version: 1
direction: direction/forward
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation988751
display id: annotation988751
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location: https://synbiohub.org/public/igem/BBa_I9128/annotation988751/range988751/1
component: BBa_B0012

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creator: crackdots
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modified: 2015-08-31T04:08:11Z
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description: RBS.GFP + PBad CFP
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part status: Released HQ 2013
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status: status/Available
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owned by: myers
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component: BBa_I0500
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component: BBa_B0034
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sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_E0020
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type: ComponentDefinition
persistent identity: BBa I13977
display id: BBa_I13977
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac relaxation oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: intermediate part for the lac version of the synchronized relaxation oscillator
mutable notes: Sequence verified, however there is an N at position 1021, however upon looking at the actual sequence trace it looks like it is most likely the correct base.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_S3511
component: BBa_I13976
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sequence annotation: BBa_S3511
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access: public
definition: https://synbiohub.org/public/igem/BBa_S3511/1

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definition: BBa_I13976

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type: ComponentDefinition
persistent identity: BBa J06950
display id: BBa_J06950
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda cI/LacI RS flip-flop
creator: ytwang
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with LacI as the set and lambda cI as the reset.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0051
component: BBa_E2060
component: BBa_B0010
component: BBa_B0012
component: BBa_R0051
component: BBa_B0034
component: BBa_C0012
component: BBa_R0011
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sequence annotation: BBa_B0034
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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BBa_I2011
type: ComponentDefinition
persistent identity: BBa I2011
display id: BBa_I2011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B_Receiver Device (R1075, B0034.C0071.B0015)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter, RBS, rhlR coding region, terminator rhlR protein binds AI-2 chemical signal to induce operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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description: Ring Oscillator Synchronization Device (lac)
creator: jasonk
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
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experience: experience/None
group u list: _11_6_
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sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is meant to be coupled to the elowitz repressilator plasmid to synchronization oscillations accross a population of cells. Sequence
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description: Booperator: Lux/Rhl, monocistronic
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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sample status: Not in stock
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BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0052
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definition: https://synbiohub.org/public/igem/BBa_R0052/1

BBa_C0061
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definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0071
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definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_R0051
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0070
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definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_C0050
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definition: https://synbiohub.org/public/igem/BBa_C0050/1

BBa_R0040
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
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definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0071
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definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_C0061
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definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_R0071
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BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
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definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
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definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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display id: component1012658
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
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definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0040
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definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_C0051
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definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
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display id: component1012830
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0071
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display id: component1012516
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definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0034
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display id: component1012371
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0050
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definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_B0034
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display id: component1012679
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
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definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
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display id: component1012845
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definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_C0052
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display id: component1012578
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definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_C0051
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display id: component1012531
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definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_R0071
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BBa_B0034
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BBa_B0012
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BBa_B0010
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BBa_B0010
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BBa_R0051
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BBa_B0034
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BBa_B0012
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BBa_B0012
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BBa_B0012
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BBa_R0071
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BBa_R0050
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BBa_R0040
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BBa_C0061
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BBa_B0010
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BBa_B0010
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BBa_C0040
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BBa_E0032
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BBa_R0052
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BBa_C0061
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BBa_C0062
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BBa_B0012
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BBa_B0034
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display id: BBa_I15032
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL amplifier + reporter device
creator: Jeffrey J Tabor
created: 2005-09-06T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0065
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description: Synchronator design 4, Output-
creator: SMUG: Oscillator
created: 2004-08-15T11:00:00Z
modified: 2015-08-31T04:07:41Z
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experience: experience/None
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mutable description: -- No description --
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Tar-EnvZ attractant receiver (I3301.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests Tar-EnvZ attractant receiver device (truncated ompC upstream version) by attaching TIPS output to CFP reporter.
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owned by: myers
star: false
type: DnaRegion
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type: ComponentDefinition
persistent identity: BBa I3302
display id: BBa_I3302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0084)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive promoter makes Tar-EnvZ fusion protein, which binds aspartate to create TIPS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R1075
component: BBa_R0084
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sequence annotation: BBa_C0082
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access: public
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persistent identity: BBa I13975
display id: BBa_I13975
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac synchro device
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part in lac synchronization device
mutable notes: This part is the result of a build step which was done backwards by accident. The correctly orientated version was remade as I13978
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_C0012
component: BBa_C0061
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component: BBa_R0062
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definition: https://synbiohub.org/public/igem/BBa_C0061/1

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version: 1
top level: BBa_I13975
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

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orientation: inline

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end: 1146
orientation: inline

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direction: direction/forward
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orientation: inline

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display id: range1426865
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direction: direction/forward
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type: ComponentDefinition
persistent identity: BBa I9109
display id: BBa_I9109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (lambda-cI nor_input) I0500.E0422.I0500.I9009
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_E0022
component: BBa_I0500
component: BBa_I9200
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
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sequence annotation: BBa_B0034
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sequence annotation: BBa_I9200
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sequence: https://synbiohub.org/public/igem/BBa_I9109_sequence/1

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version: 1
top level: BBa_I9109
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component968967
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definition: https://synbiohub.org/public/igem/BBa_I0500/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_I9200/1

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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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version: 1
top level: BBa_I9109
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definition: https://synbiohub.org/public/igem/BBa_B0010/1

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display id: component969018
version: 1
top level: BBa_I9109
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
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orientation: inline

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version: 1
top level: BBa_I9109
location: https://synbiohub.org/public/igem/BBa_I9109/annotation968950/range968950/1
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orientation: inline

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version: 1
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version: 1
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3701.I3300.I370
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: E. colibrator design 2 has an explicit OR gate before the switch via an araC OR gate device (I3701, I3700). Design 2 has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
mutable notes: The layout of E. colibrator (I3001) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls the araC input device for the OR gate (I3100.I3701), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the second OR input, embedded in the araC buffer device (I3300.I3700). The araC OR gate in turn outputs TIPS into the hk022cI switch input (I3400). Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
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description: Booperator test: Inverter NOR biasing in polycistronic design
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
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owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the fourth test chassis for the Booperator and is particular to the polycistronic design. It tests the biasing of the Inverter NOR pair.
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modified: 2015-08-31T04:07:39Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Quorum threshold level and chemotaxis response, receiver cells (I3601.I3520.I3500)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.
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owned by: myers
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR generator. POPS generate cI(LVA) and LuxI(LVA)
creator: Jeff Tabor
created: 2005-04-16T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Accepts POPS to produce lambda cI (LVA) and LuxI (LVA). Constitive LuxR generator in the absence of TetR.
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owned by: myers
star: false
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role: SO:0000804
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component: BBa_B0034
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component: BBa_C0061
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component: BBa_R0040
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top level: BBa_J13001
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BBa_J13030
type: ComponentDefinition
persistent identity: BBa J13030
display id: BBa_J13030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 amplifier
creator: Laura Lavery
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 327
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This circuit is regulated by two proteins, TetR and CI. In the presence of TetR, LasR production will be repressed. In the presence of CI, LasI production will be repressed. Without repressors present the circuit may be leaky enough to trigger the positive feedback LasI/LasR/PAI response.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_C0078
component: BBa_B0034
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component: BBa_I14016
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sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_I14016
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sequence: https://synbiohub.org/public/igem/BBa_J13030_sequence/1

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type: ComponentDefinition
persistent identity: BBa I3600
display id: BBa_I3600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Chemotaxis response to secreted aspartate, sender cells (I3100.I3200.E0422)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
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type: ComponentDefinition
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display id: BBa_I15002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI regulated cI/LuxI/LacZalpha
creator: jtabor
created: 2004-05-22T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: true
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Tester module for BBa_I15001. Flanked by dual terminators. LacI regulated polycistronic cI/LuxI/LacZalpha
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owned by: myers
star: false
type: DnaRegion
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component: BBa_B0012
component: BBa_I1020
component: BBa_B0010
component: BBa_B0034
component: BBa_E0033
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_I1020
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display id: BBa_I8102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR, LuxR, and LasR constitutive generation (using TetR)
creator: Ronny Krashinsky
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_C0079
component: BBa_C0071
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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component: BBa_C0071

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component: BBa_R0040

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BBa_R0040
type: SequenceAnnotation
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display id: annotation990550
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component: BBa_R0040

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display id: range990550
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end: 964
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type: ComponentDefinition
persistent identity: BBa I4203
display id: BBa_I4203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash *: Synchronator Design 3
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I4204
component: BBa_Q04400
component: BBa_R0062
component: BBa_Q04511
component: BBa_Q04121
sequence annotation: BBa_Q04400
sequence annotation: BBa_Q04121
sequence annotation: BBa_Q04511
sequence annotation: BBa_I4204
sequence annotation: BBa_R0062
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access: public
definition: BBa_I4204

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type: Component
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display id: component2241505
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

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persistent identity: component2241483
display id: component2241483
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

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display id: component2241545
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_Q04511/1

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type: Component
persistent identity: component2241524
display id: component2241524
version: 1
top level: BBa_I4203
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

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type: SequenceAnnotation
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display id: annotation2241505
version: 1
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location: https://synbiohub.org/public/igem/BBa_I4203/annotation2241505/range2241505/1
component: BBa_Q04400

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type: SequenceAnnotation
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version: 1
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component: BBa_Q04121

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display id: range2241524
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type: SequenceAnnotation
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display id: annotation2241545
version: 1
top level: BBa_I4203
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component: BBa_I4204

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BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2241483
display id: annotation2241483
version: 1
top level: BBa_I4203
location: https://synbiohub.org/public/igem/BBa_I4203/annotation2241483/range2241483/1
component: BBa_R0062

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persistent identity: range2241483
display id: range2241483
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start: 1
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BBa_I3700
type: ComponentDefinition
persistent identity: BBa I3700
display id: BBa_I3700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC buffer device for OR gate (I3701.R0080)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positive regulation of araC promoter by AraC CDS. This buffer takes TIPS in as one of two OR gate inputs and outputs TIPS. The second input to the OR gate is I3701.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0080
component: BBa_R0080
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_C0080
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_I3700_sequence/1

BBa_C0080
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display id: component947693
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_C0080/1

BBa_R0080
type: Component
persistent identity: component947732
display id: component947732
version: 1
top level: BBa_I3700
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_B0012
type: Component
persistent identity: component947709
display id: component947709
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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display id: component947683
version: 1
top level: BBa_I3700
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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persistent identity: component947699
display id: component947699
version: 1
top level: BBa_I3700
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

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type: SequenceAnnotation
persistent identity: annotation947693
display id: annotation947693
version: 1
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orientation: inline

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display id: annotation947699
version: 1
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component: BBa_B0010

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display id: range947699
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orientation: inline

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type: SequenceAnnotation
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display id: annotation947683
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component: BBa_B0034

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start: 1
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orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947709
display id: annotation947709
version: 1
top level: BBa_I3700
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component: BBa_B0012

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type: Range
persistent identity: range947709
display id: range947709
version: 1
direction: direction/forward
top level: BBa_I3700
start: 1055
end: 1095
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation947732
display id: annotation947732
version: 1
top level: BBa_I3700
location: https://synbiohub.org/public/igem/BBa_I3700/annotation947732/range947732/1
component: BBa_R0080

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type: Range
persistent identity: range947732
display id: range947732
version: 1
direction: direction/forward
top level: BBa_I3700
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orientation: inline

BBa_I12059
type: ComponentDefinition
persistent identity: BBa I12059
display id: BBa_I12059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12036 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12036. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I12054
component: BBa_I12024
component: BBa_E0434
component: BBa_I12056
sequence annotation: BBa_I12024
sequence annotation: BBa_I12054
sequence annotation: BBa_E0434
sequence annotation: BBa_I12056
sequence: https://synbiohub.org/public/igem/BBa_I12059_sequence/1

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persistent identity: component2237703
display id: component2237703
version: 1
top level: BBa_I12059
access: public
definition: https://synbiohub.org/public/igem/BBa_I12054/1

BBa_I12024
type: Component
persistent identity: component2237685
display id: component2237685
version: 1
top level: BBa_I12059
access: public
definition: https://synbiohub.org/public/igem/BBa_I12024/1

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type: Component
persistent identity: component2237742
display id: component2237742
version: 1
top level: BBa_I12059
access: public
definition: https://synbiohub.org/public/igem/BBa_E0434/1

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persistent identity: component2237732
display id: component2237732
version: 1
top level: BBa_I12059
access: public
definition: https://synbiohub.org/public/igem/BBa_I12056/1

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persistent identity: annotation2237685
display id: annotation2237685
version: 1
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component: BBa_I12024

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persistent identity: range2237685
display id: range2237685
version: 1
direction: direction/reverse
top level: BBa_I12059
start: 1
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2237703
display id: annotation2237703
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237703/range2237703/1
component: BBa_I12054

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persistent identity: range2237703
display id: range2237703
version: 1
direction: direction/reverse
top level: BBa_I12059
start: 1002
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2237742
display id: annotation2237742
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237742/range2237742/1
component: BBa_E0434

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type: Range
persistent identity: range2237742
display id: range2237742
version: 1
direction: direction/reverse
top level: BBa_I12059
start: 3027
end: 3943
orientation: inline

BBa_I12056
type: SequenceAnnotation
persistent identity: annotation2237732
display id: annotation2237732
version: 1
top level: BBa_I12059
location: https://synbiohub.org/public/igem/BBa_I12059/annotation2237732/range2237732/1
component: BBa_I12056

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type: Range
persistent identity: range2237732
display id: range2237732
version: 1
direction: direction/reverse
top level: BBa_I12059
start: 1891
end: 3018
orientation: inline

BBa_I9113
type: ComponentDefinition
persistent identity: BBa I9113
display id: BBa_I9113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test (wired_nor_switch 2) I0500.E0422.I0500.I9003.I9004.E0432
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_I0500
component: BBa_E0032
component: BBa_B0010
component: BBa_I9201
component: BBa_I9200
component: BBa_B0012
component: BBa_R0075
component: BBa_E0022
component: BBa_C0075
sequence annotation: BBa_B0010
sequence annotation: BBa_I9200
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_R0075
sequence annotation: BBa_E0022
sequence annotation: BBa_I9201
sequence annotation: BBa_C0075
sequence: https://synbiohub.org/public/igem/BBa_I9113_sequence/1

BBa_B0034
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persistent identity: component990958
display id: component990958
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
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persistent identity: component990902
display id: component990902
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0032
type: Component
persistent identity: component991006
display id: component991006
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
type: Component
persistent identity: component990994
display id: component990994
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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persistent identity: component990887
display id: component990887
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I9201
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persistent identity: component990989
display id: component990989
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_I9201/1

BBa_B0010
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display id: component991014
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: component990971
display id: component990971
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
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persistent identity: component990862
display id: component990862
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I9200
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persistent identity: component990965
display id: component990965
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_I9200/1

BBa_B0012
type: Component
persistent identity: component991024
display id: component991024
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component990981
display id: component990981
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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persistent identity: component990923
display id: component990923
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
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persistent identity: component990867
display id: component990867
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0075
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persistent identity: component990953
display id: component990953
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_R0075/1

BBa_B0012
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persistent identity: component990897
display id: component990897
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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persistent identity: component990933
display id: component990933
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component990907
display id: component990907
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
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persistent identity: component990879
display id: component990879
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_C0075
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persistent identity: component990917
display id: component990917
version: 1
top level: BBa_I9113
access: public
definition: https://synbiohub.org/public/igem/BBa_C0075/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation990887
display id: annotation990887
version: 1
top level: BBa_I9113
location: https://synbiohub.org/public/igem/BBa_I9113/annotation990887/range990887/1
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persistent identity: range990887
display id: range990887
version: 1
direction: direction/forward
top level: BBa_I9113
start: 2007
end: 2086
orientation: inline

BBa_I9200
type: SequenceAnnotation
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display id: annotation990965
version: 1
top level: BBa_I9113
location: https://synbiohub.org/public/igem/BBa_I9113/annotation990965/range990965/1
component: BBa_I9200

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persistent identity: range990965
display id: range990965
version: 1
direction: direction/forward
top level: BBa_I9113
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display id: annotation990971
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location: https://synbiohub.org/public/igem/BBa_I9113/annotation990971/range990971/1
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persistent identity: range990971
display id: range990971
version: 1
direction: direction/forward
top level: BBa_I9113
start: 5022
end: 5101
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991014
display id: annotation991014
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description: XOR test/debug module
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
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sample status: Not in stock
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bookmark: false
mutable description: This part combines multiple Biobrick elements into a single module for testing and debugging elements of the XOR genetic gate. It tests systems that use cI protein as output. The promoter within the testing module is cI repressible. The advantage of constructing the test module de novo is the ease of transferring the entire unit to elements we would like to test within the XOR gate. YFP expression levels are the output of the test module and serve as a marker for system function.
mutable notes: synthetic element, using a combination of Biobrick parts--cI promoter, RBS, YFP, transcriptional terminators. used for testing systems that use cI protein as outputs.
owned by: james
owned by: myers
star: false
type: DnaRegion
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Olmecs: Oscillator 1 of Synchro-Dualator
creator: Olmecs 2003 IAP Team
created: 2003-11-27T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: For reference only
status: status/Informational
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
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display id: BBa_I13912
version: 1
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description: AiiA (+LVA) Tri-part (B0032.C0060.B0015)
creator: robertb
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS + AiiA (+LVA) + Term
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
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component: BBa_B0012
component: BBa_B0032
component: BBa_B0010
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persistent identity: BBa I12038
display id: BBa_I12038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kickstart for the Barkai-Leibler oscillator
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is designed for "kickstarting" the Barkai-Leibler oscillator by producing lambda cI.
mutable notes: Addition of ATC will repress the tetR and this will result in production of lambda cI.
mutable provenance: BioBricks parts
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owned by: myers
star: false
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display id: BBa_I3301
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0083)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
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modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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status: status/Unavailable
bookmark: false
mutable description: Constitutive promoter makes Tar-EnvZ fusion protein, which binds aspartate to create TIPS.
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owned by: myers
star: false
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persistent identity: BBa I13021
display id: BBa_I13021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Test Construct Intermediate (Q04121.E0430)
creator: Barry Canton
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0430
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exogenous AiiA Relaxation Oscillator Test Construct Intermediate (R0062.B0034.C0061.E0434)
creator: robertb
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modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Intermediate construct for Chris' relaxation oscillator test construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0034
component: BBa_R0062
component: BBa_C0061
component: BBa_B0034
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BL oscillator (I12040 based) with IPTG kickstart
creator: endelman
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified BL oscillator based on I12040. It can be "reset" by adding IPTG which stimulates production of the A protein (lambda cI) if used in LacIq strain. The B protein is 434 cI. The YFP-AAV reporter tracks the dynamics of the A protein.
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display id: BBa_J13011
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressed Cph8
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
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m user id: 0
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status: status/Unavailable
bookmark: false
mutable description: -- No description --
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display id: BBa_I4002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash: Design 2
creator: Randy Rettberg
created: 2003-12-03T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
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BBa_R0040
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description: Relaxation Oscillator Test Construct (I13202.B0015.I0460)
creator: robertb
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description: QPI Test Construct Intermediate (Q01121.E0430)
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3302.I3400.I3102.I340
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3302 ompF upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3302 upstream ompF version) controls the other (I3302.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for I12007
creator: endelman
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 145
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: When used in LacIq strain, addition of IPTG should increase YFP fluorescence if I12007 is working.
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owned by: myers
star: false
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display id: BBa_J06965
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (Lambda II) RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RS flip-flop with lambda cI857 (Lambda II) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
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owned by: myers
star: false
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role: SO:0000804
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prakash: Design 4
creator: Randy Rettberg
created: 2003-12-03T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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sample status: Not in stock
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owned by: james
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modified: 2015-08-31T04:08:11Z
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group u list: _1_
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modified: 2015-08-31T04:07:32Z
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mutable description: -- No description --
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display id: BBa_I4211
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description: Synchronator design 1, Output-
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modified: 2015-08-31T04:07:41Z
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component: BBa_R0011
component: BBa_I0462
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sequence annotation: BBa_Q04400
sequence annotation: BBa_F1610
sequence annotation: BBa_R0065
sequence annotation: BBa_I0460
sequence annotation: BBa_Q04121
sequence annotation: BBa_R0011
sequence annotation: BBa_R0063
sequence annotation: BBa_Q04511
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definition: https://synbiohub.org/public/igem/BBa_Q04400/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

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component: BBa_R0065

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component: BBa_I0460

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type: ComponentDefinition
persistent identity: BBa I3601
display id: BBa_I3601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Chemotaxis response to secreted aspartate, receiver cells (R1075.E0432)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests the chemotaxis cell-cell signalling machinery based on aspartate secretions. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
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component: BBa_B0010
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sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R1075
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I3601_sequence/1

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BBa_I3424
type: ComponentDefinition
persistent identity: BBa I3424
display id: BBa_I3424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test: Switch response to quorum (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3510.I3401)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tests ability of switch to respond to quorum sensing input by connecting the quorum receiver to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum receiver device controls the lambda cI input (I3510.I3401).
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star: false
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component: BBa_R0062
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component: BBa_E0032
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_C0050/1

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top level: BBa_I3424
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definition: https://synbiohub.org/public/igem/BBa_E0032/1

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display id: component1008025
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top level: BBa_I3424
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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definition: https://synbiohub.org/public/igem/BBa_B0034/1

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experience: experience/None
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modified: 2015-08-31T04:08:11Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator: LuxR/RhlR/aiia, monocistronic
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-30T12:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
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sample status: Not in stock
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description: Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
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mutable description: Tests cellular density of asparate-secreting cells needed to induce quorum threshold response. Sender cells are nonmotile and have a trigger controlling the AspA attractant sender and CFP reporter indicating transcription of aspA. Receiver cells have a constitutive promoter controlling YFP reporter. Chemotaxis can be observed by the movement of the YFP-marked receiver cells. Quorum threshold is detected when the LacZ reporter is observed.
mutable notes: Sender cells are the nonchemotactic E. coli strain RP5232. Receiver cells are wildtype E. coli. This test combines the chemotaxis response test with the quorum threshold test.
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modified: 2015-08-31T04:08:15Z
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description: 434 cI/lambda cI RS flip-flop
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modified: 2015-08-31T04:08:19Z
discontinued: false
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Booperator test: LuxR biasing, LuxR/RhlR/LasR crosstalk
creator: Ronny Krashinsky
created: 2004-01-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 434 cI/lambda cI857 (E07700) RS flip-flop with EYFP
creator: Yves Wang
created: 2005-07-31T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: true
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: RS flip-flop with lambda cI (E07700) as the set and 434 cI as the reset. It will also take UV exposure as the set and heat (40??C) as the reset.
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owned by: myers
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component: BBa_I9201

https://synbiohub.org/public/igem/BBa_I9104/annotation981435/range981435/1
type: Range
persistent identity: range981435
display id: range981435
version: 1
direction: direction/forward
top level: BBa_I9104
start: 4267
end: 4348
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation981460
display id: annotation981460
version: 1
top level: BBa_I9104
location: https://synbiohub.org/public/igem/BBa_I9104/annotation981460/range981460/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9104/annotation981460/range981460/1
type: Range
persistent identity: range981460
display id: range981460
version: 1
direction: direction/forward
top level: BBa_I9104
start: 5145
end: 5224
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981470
display id: annotation981470
version: 1
top level: BBa_I9104
location: https://synbiohub.org/public/igem/BBa_I9104/annotation981470/range981470/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9104/annotation981470/range981470/1
type: Range
persistent identity: range981470
display id: range981470
version: 1
direction: direction/forward
top level: BBa_I9104
start: 5233
end: 5273
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981394
display id: annotation981394
version: 1
top level: BBa_I9104
location: https://synbiohub.org/public/igem/BBa_I9104/annotation981394/range981394/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9104/annotation981394/range981394/1
type: Range
persistent identity: range981394
display id: range981394
version: 1
direction: direction/forward
top level: BBa_I9104
start: 2095
end: 2135
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981427
display id: annotation981427
version: 1
top level: BBa_I9104
location: https://synbiohub.org/public/igem/BBa_I9104/annotation981427/range981427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9104/annotation981427/range981427/1
type: Range
persistent identity: range981427
display id: range981427
version: 1
direction: direction/forward
top level: BBa_I9104
start: 4218
end: 4258
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation981440
display id: annotation981440
version: 1
top level: BBa_I9104
location: https://synbiohub.org/public/igem/BBa_I9104/annotation981440/range981440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9104/annotation981440/range981440/1
type: Range
persistent identity: range981440
display id: range981440
version: 1
direction: direction/forward
top level: BBa_I9104
start: 4357
end: 4368
orientation: inline

BBa_I12000
type: ComponentDefinition
persistent identity: BBa I12000
display id: BBa_I12000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barkai-Leibler design experiment Part A (Lambda cI)
creator: mcnamara
created: 2004-07-11T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This forms the activator of a Barkai-Leibler oscillator.
mutable notes: This part to work in conjunction with BBa_I12002. This part creates one of the proteins involved in the Modified Barkai-Leibler relaxation oscillator design (activator protein: lambda cI). In the presence of Lambda cI, more Lambda cI will be produced (positively autoregulated). However, if there is 434 cI repressor present in the system, the production of Lambda cI should be repressed. This was implemented in the subpart BBa_I12006, which represents the Operating Regions for the promoter. The fact that the OR-3 site was replaced with the OR-1 site of 434 cI repressor theoretically should mean that when 434 cI binds in, the production of the Lambda cI protein should be blocked.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: https://synbiohub.org/public/igem/BBa_I12000/component2223470/1
component: https://synbiohub.org/public/igem/BBa_I12000/component2223451/1
component: https://synbiohub.org/public/igem/BBa_I12000/component2223463/1
component: https://synbiohub.org/public/igem/BBa_I12000/component2223457/1
component: https://synbiohub.org/public/igem/BBa_I12000/component2223459/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I12000/annotation2223470/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I12000/annotation2223459/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I12000/annotation2223451/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I12000/annotation2223457/1
sequence annotation: https://synbiohub.org/public/igem/BBa_I12000/annotation2223463/1
sequence: https://synbiohub.org/public/igem/BBa_I12000_sequence/1