phage 434 represses and lambda activates
Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)
This is a device that enable the control of chez(histag) using IPTG. The pLacIq promoter enables high level of expression of the LacI repressor protein. The LacI protein represses the Plac promoter. IPTG binds with LacI repressor protein and remove the inhibiting effect of the LacI on the pLac promoter.
The rbs, terminator and the pLac promoter are from the registry, the rest of the part are self designed
ToxR'::scFv Fusion 2 (with promoter and rbs and terminator)
see description for J07024. This scFv is somewhat stronger than the one used in J07024.
Ring Oscillator Synchronization Device (lac)
This device is meant to be coupled to the elowitz repressilator device to synchronize a population of oscillating cells.
This part is the one of the responding systems of the receiver device of our Photocommunication system
We used ncbi when we couldn't find sequence
EPIC Luciferase: BBa_K325100, Cambridge 2010 pOmpC: BBa_R0082
The 4-State Device is a toggle switch that sequenctially progresses throuh 4 states with alternating inputs from Pr and Prm. It can be used to function in a counter. The interface promotors from the EPD are included.
ToxR'::scFv Fusion 1 (with promoter and rbs and terminator)
The first of three fusions between ToxR', a modified dimerizing protein, and an anti-fluorescein binding scFv sequence. ToxR', active as a dimer, binds to the ctx promoter sequence (BBa_J07007) to initiate transcription. This part is designed to respond to a fluorescein dimer and activate the ctx promoter. The restriction sites flagged below were introduced to allow for protein fusions, and are not to be removed. See ToxR' (J07009) for details.
This is a potential synchronization oscillator similar to the synchonization element of the synchronized ring oscillator device.
BBa_J04900 (3AptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
Andrew Drysdale and Kristen DeCelle (also Oscar Hernandez and Matt Gemberling)
This is a (3AptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
Amplifies TIPS signal and produces lambda.
BBa_J04700 (ForAptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
This is a (ForAptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
This part contains a strong rbs and a TetR tagged with a LVA degradation tail. The TetR repressor can be used to repress ptet promoter and the TetR coding region do not contain any terminator
BBa_J04800 (RevAptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
This is a (RevAptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
ToxR'::scFv Fusion 3 (with promoter and rbs and terminator)
See description for J07024. This scFv sequence binds most strongly to fluorescein molecules.