//chassis/prokaryote/subtilis
type: Collection
persistent identity: chassis prokaryote subtilis
display id: chassis_prokaryote_subtilis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K823014
member: BBa_K823008
member: BBa_K823006
member: PcotYZ
member: BBa_K823005
member: Pxyl
member: BBa_K090501
member: BBa_K823013
member: BBa_K823011
member: pSBBs0K
member: spac+efe
member: beta
member: BBa_K823007
member: Pctc
member: BBa_K823004
member: P43
member: BBa_K090504
member: PgsiB
member: Ph-s
member: BBa_K823010

Pctc
type: ComponentDefinition
persistent identity: BBa K143010
display id: BBa_K143010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter ctc for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter ctc is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2. *The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3. ===Reference=== <biblio> #1 pmid=16267279 #2 pmid=3100810 #3 pmid=17575448 </biblio>
mutable notes: Biobrick standard was applied to the promoter ctc sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143010_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975700
display id: annotation1975700
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1
type: Range
persistent identity: range1975700
display id: range1975700
version: 1
direction: direction/forward
top level: Pctc
start: 41
end: 46
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975701
display id: annotation1975701
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1
type: Range
persistent identity: range1975701
display id: range1975701
version: 1
direction: direction/forward
top level: Pctc
start: 21
end: 28
orientation: inline

BBa_K823004
type: ComponentDefinition
persistent identity: BBa K823004
display id: BBa_K823004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23100
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:43:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823004_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2182506
display id: annotation2182506
version: 1
top level: BBa_K823004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1
type: Range
persistent identity: range2182506
display id: range2182506
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182508
display id: annotation2182508
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1
type: Range
persistent identity: range2182508
display id: range2182508
version: 1
direction: direction/forward
top level: BBa_K823004
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182507
display id: annotation2182507
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1
type: Range
persistent identity: range2182507
display id: range2182507
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 6
orientation: inline

BBa_K090504
type: ComponentDefinition
persistent identity: BBa K090504
display id: BBa_K090504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive Strong Constitutive Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli. The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
mutable notes: This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
mutable provenance: We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: putative ComK binding region
sequence annotation: putative SigA -35
sequence annotation: putative SigA -10
sequence: https://synbiohub.org/public/igem/BBa_K090504_sequence/1

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation1991955
display id: annotation1991955
version: 1
top level: BBa_K090504
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
type: Range
persistent identity: range1991955
display id: range1991955
version: 1
direction: direction/forward
top level: BBa_K090504
start: 76
end: 88
orientation: inline

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation1991956
display id: annotation1991956
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
type: Range
persistent identity: range1991956
display id: range1991956
version: 1
direction: direction/forward
top level: BBa_K090504
start: 102
end: 108
orientation: inline

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation1991957
display id: annotation1991957
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
type: Range
persistent identity: range1991957
display id: range1991957
version: 1
direction: direction/forward
top level: BBa_K090504
start: 125
end: 130
orientation: inline

PgsiB
type: ComponentDefinition
persistent identity: BBa K143011
display id: BBa_K143011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter gsiB for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter gsiB is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter gsiB has been used previously as a read out for the activation of sigma factor B 2. The context with which we used the promoter gsiB, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3.
mutable notes: Biobrick standard was applied to the promoter gsiB sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143011_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975703
display id: annotation1975703
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1
type: Range
persistent identity: range1975703
display id: range1975703
version: 1
direction: direction/forward
top level: PgsiB
start: 25
end: 30
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975702
display id: annotation1975702
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1
type: Range
persistent identity: range1975702
display id: range1975702
version: 1
direction: direction/forward
top level: PgsiB
start: 6
end: 11
orientation: inline

BBa_K823013
type: ComponentDefinition
persistent identity: BBa K823013
display id: BBa_K823013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23117
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23117
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182544
display id: annotation2182544
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1
type: Range
persistent identity: range2182544
display id: range2182544
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 6
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2182543
display id: annotation2182543
version: 1
top level: BBa_K823013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1
type: Range
persistent identity: range2182543
display id: range2182543
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182545
display id: annotation2182545
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1
type: Range
persistent identity: range2182545
display id: range2182545
version: 1
direction: direction/forward
top level: BBa_K823013
start: 24
end: 29
orientation: inline

pSBBs0K
type: ComponentDefinition
persistent identity: BBa K823026
display id: BBa_K823026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid). This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pDG148
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacI
sequence annotation: kan
sequence annotation: Pspac
sequence annotation: bla
sequence annotation: lacY
sequence annotation: ble
sequence annotation: repU
sequence: https://synbiohub.org/public/igem/BBa_K823026_sequence/1

lacI
type: SequenceAnnotation
persistent identity: annotation2180256
display id: annotation2180256
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
type: Range
persistent identity: range2180256
display id: range2180256
version: 1
direction: direction/forward
top level: pSBBs0K
start: 271
end: 1353
orientation: inline

kan
type: SequenceAnnotation
persistent identity: annotation2180259
display id: annotation2180259
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
type: Range
persistent identity: range2180259
display id: range2180259
version: 1
direction: direction/forward
top level: pSBBs0K
start: 5257
end: 6027
orientation: inline

Pspac
type: SequenceAnnotation
persistent identity: annotation2180262
display id: annotation2180262
version: 1
top level: pSBBs0K
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
type: Range
persistent identity: range2180262
display id: range2180262
version: 1
direction: direction/forward
top level: pSBBs0K
start: 8193
end: 8258
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180257
display id: annotation2180257
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
type: Range
persistent identity: range2180257
display id: range2180257
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 2609
end: 3469
orientation: inline

lacY
type: SequenceAnnotation
persistent identity: annotation2180261
display id: annotation2180261
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
type: Range
persistent identity: range2180261
display id: range2180261
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 6906
end: 7871
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2180260
display id: annotation2180260
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
type: Range
persistent identity: range2180260
display id: range2180260
version: 1
direction: direction/forward
top level: pSBBs0K
start: 6250
end: 6648
orientation: inline

repU
type: SequenceAnnotation
persistent identity: annotation2180258
display id: annotation2180258
version: 1
top level: pSBBs0K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
type: Range
persistent identity: range2180258
display id: range2180258
version: 1
direction: direction/forward
top level: pSBBs0K
start: 4093
end: 5097
orientation: inline

beta
type: ComponentDefinition
persistent identity: BBa K1716000
display id: BBa_K1716000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda Beta recombinase optimized for expression in B. subtilis
creator: Pernille Neve Myers
created: 2015-09-10T11:00:00Z
modified: 2015-09-17T02:30:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2136_
m user id: 25211
owner id: 25211
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Recombination-mediated genetic engineering (recombineering) utlises homologous recombination to facilitate genetic modifications at any desired target by flanking the mutated sequence with homologous regions. Multiplex Automated Genome Engineering (MAGE) is a method for rapid and efficient targeted programming and evolution of cells through cyclical recombineering using multiple single-stranded DNA oligonucleotides (oligos). The MAGE protocol utilises the λ Red recombination system in combination with an (temporary) inactivation of the mismatch repair system and consists of 7 steps that can be done with standard laboratory equipment (Wang, 2009). As MAGE utilises oligos, only the Beta protein of the λ Red system is required. This BioBrick encodes an optimised coding sequence for lambda phage derived beta recombinase. When expressed in Bacillus subtilis, oligo recombineering efficiencies were increased. (Please see results).
mutable notes: The original nucleotide sequence from E. coli () was optimized for expression in Bacillus subtilis using JCat
mutable provenance: Gene product from E. coli phage lambda.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1716000_sequence/1

BBa_K090501
type: ComponentDefinition
persistent identity: BBa K090501
display id: BBa_K090501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive IPTG-Inducible Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
mutable notes: We found the functional annotation in the associated paper: Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628 The primers we used to extract it (sans biobrick ends) are as follow: Forward: AGAACAACCTCTGCTAAAATTC Reverse: AAGCTTAATTGTTATCCGCTC
mutable provenance: We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: lacOid Operator
sequence: https://synbiohub.org/public/igem/BBa_K090501_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1991823
display id: annotation1991823
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1
type: Range
persistent identity: range1991823
display id: range1991823
version: 1
direction: direction/forward
top level: BBa_K090501
start: 66
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1991822
display id: annotation1991822
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1
type: Range
persistent identity: range1991822
display id: range1991822
version: 1
direction: direction/forward
top level: BBa_K090501
start: 43
end: 48
orientation: inline

lacOid Operator
type: SequenceAnnotation
persistent identity: annotation1991824
display id: annotation1991824
version: 1
top level: BBa_K090501
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1
type: Range
persistent identity: range1991824
display id: range1991824
version: 1
direction: direction/forward
top level: BBa_K090501
start: 78
end: 97
orientation: inline

BBa_K823014
type: ComponentDefinition
persistent identity: BBa K823014
display id: BBa_K823014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23118
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: J23118
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823014_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182548
display id: annotation2182548
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1
type: Range
persistent identity: range2182548
display id: range2182548
version: 1
direction: direction/forward
top level: BBa_K823014
start: 24
end: 29
orientation: inline

J23118
type: SequenceAnnotation
persistent identity: annotation2182546
display id: annotation2182546
version: 1
top level: BBa_K823014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1
type: Range
persistent identity: range2182546
display id: range2182546
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182547
display id: annotation2182547
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1
type: Range
persistent identity: range2182547
display id: range2182547
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 6
orientation: inline

Pxyl
type: ComponentDefinition
persistent identity: BBa K143014
display id: BBa_K143014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Xyl for B.subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter Xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Promoter xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator '''XylR''', (<bbpart>BBa_K143036</bbpart>) This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter xylose.
mutable notes: Biobrick standard was applied to the promoter xylose sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 12. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence annotation: XylR Operator
sequence: https://synbiohub.org/public/igem/BBa_K143014_sequence/1

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976429
display id: annotation1976429
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
type: Range
persistent identity: range1976429
display id: range1976429
version: 1
direction: direction/forward
top level: Pxyl
start: 36
end: 41
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976428
display id: annotation1976428
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
type: Range
persistent identity: range1976428
display id: range1976428
version: 1
direction: direction/forward
top level: Pxyl
start: 13
end: 18
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975869
display id: annotation1975869
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
type: Range
persistent identity: range1975869
display id: range1975869
version: 1
direction: direction/forward
top level: Pxyl
start: 65
end: 75
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975868
display id: annotation1975868
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
type: Range
persistent identity: range1975868
display id: range1975868
version: 1
direction: direction/forward
top level: Pxyl
start: 51
end: 61
orientation: inline

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23102
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23102
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 6
orientation: inline

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
direction: direction/forward
top level: BBa_K823006
start: 24
end: 29
orientation: inline

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23101
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23101
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
direction: direction/forward
top level: BBa_K823005
start: 24
end: 29
orientation: inline

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 35
orientation: inline

PcotYZ
type: ComponentDefinition
persistent identity: BBa K823030
display id: BBa_K823030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcotYZ: B. subtilis promoter regulating expression of spore crust proteins
creator: Franziska D??rr
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12084
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PcotYZ: B. subtilis promoter regulates spore crust proteins and is activated in late sporulation. PcotYZ regulates the crust protein cotZ and cotY.
mutable notes: We use this promoter for the expression of fusion proteins on B. subtilis spores.
mutable provenance: B. subtilis W168 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PcotYZ
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K823030_sequence/1

PcotYZ
type: SequenceAnnotation
persistent identity: annotation2182004
display id: annotation2182004
version: 1
top level: PcotYZ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823030/annotation2182004/range2182004/1

https://synbiohub.org/public/igem/BBa_K823030/annotation2182004/range2182004/1
type: Range
persistent identity: range2182004
display id: range2182004
version: 1
direction: direction/forward
top level: PcotYZ
start: 1
end: 183
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2200628
display id: annotation2200628
version: 1
top level: PcotYZ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823030/annotation2200628/range2200628/1

https://synbiohub.org/public/igem/BBa_K823030/annotation2200628/range2200628/1
type: Range
persistent identity: range2200628
display id: range2200628
version: 1
direction: direction/forward
top level: PcotYZ
start: 150
end: 157
orientation: inline

BBa_K823011
type: ComponentDefinition
persistent identity: BBa K823011
display id: BBa_K823011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23114
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:50:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: J23114
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823011_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182539
display id: annotation2182539
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1
type: Range
persistent identity: range2182539
display id: range2182539
version: 1
direction: direction/forward
top level: BBa_K823011
start: 24
end: 29
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2182537
display id: annotation2182537
version: 1
top level: BBa_K823011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1
type: Range
persistent identity: range2182537
display id: range2182537
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182538
display id: annotation2182538
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1
type: Range
persistent identity: range2182538
display id: range2182538
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 6
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23106
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
direction: direction/forward
top level: BBa_K823008
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 6
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 35
orientation: inline

P43
type: ComponentDefinition
persistent identity: BBa K143013
display id: BBa_K143013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter 43 a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within ''B.subtilis'' vectors 2. The context with which we used the promoter P43 is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 2. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence annotation: Sigma A -35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143013_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975707
display id: annotation1975707
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
type: Range
persistent identity: range1975707
display id: range1975707
version: 1
direction: direction/forward
top level: P43
start: 38
end: 47
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975706
display id: annotation1975706
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
type: Range
persistent identity: range1975706
display id: range1975706
version: 1
direction: direction/forward
top level: P43
start: 14
end: 22
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1975708
display id: annotation1975708
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
type: Range
persistent identity: range1975708
display id: range1975708
version: 1
direction: direction/forward
top level: P43
start: 24
end: 29
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975709
display id: annotation1975709
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
type: Range
persistent identity: range1975709
display id: range1975709
version: 1
direction: direction/forward
top level: P43
start: 47
end: 52
orientation: inline

spac+efe
type: ComponentDefinition
persistent identity: BBa K1065203
display id: BBa_K1065203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in pSpac (BBa_K823026)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pSpac vector that has benn sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1065002
component: BBa_K823026
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823026
sequence: https://synbiohub.org/public/igem/BBa_K1065203_sequence/1

BBa_K1065002
type: Component
persistent identity: component2334235
display id: component2334235
version: 1
top level: spac+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823026
type: Component
persistent identity: component2334243
display id: component2334243
version: 1
top level: spac+efe
access: public
definition: pSBBs0K

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334235
display id: annotation2334235
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
type: Range
persistent identity: range2334235
display id: range2334235
version: 1
direction: direction/reverse
top level: spac+efe
start: 1
end: 1215
orientation: inline

BBa_K823026
type: SequenceAnnotation
persistent identity: annotation2334243
display id: annotation2334243
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
component: BBa_K823026

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
type: Range
persistent identity: range2334243
display id: range2334243
version: 1
direction: direction/reverse
top level: spac+efe
start: 1224
end: 9512
orientation: inline

BBa_K823010
type: ComponentDefinition
persistent identity: BBa K823010
display id: BBa_K823010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23113
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K823010_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182535
display id: annotation2182535
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1
type: Range
persistent identity: range2182535
display id: range2182535
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182536
display id: annotation2182536
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1
type: Range
persistent identity: range2182536
display id: range2182536
version: 1
direction: direction/forward
top level: BBa_K823010
start: 24
end: 29
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2182534
display id: annotation2182534
version: 1
top level: BBa_K823010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1
type: Range
persistent identity: range2182534
display id: range2182534
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 35
orientation: inline

Ph-s
type: ComponentDefinition
persistent identity: BBa K143015
display id: BBa_K143015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter hyper-spank for B. subtilis
creator: Chris Hirst
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K413035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
mutable notes: Biobrick standard was applied to the promoter hyper-spank sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 123. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI Operator
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143015_sequence/1

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976423
display id: annotation1976423
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
type: Range
persistent identity: range1976423
display id: range1976423
version: 1
direction: direction/forward
top level: Ph-s
start: 10
end: 30
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976426
display id: annotation1976426
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
type: Range
persistent identity: range1976426
display id: range1976426
version: 1
direction: direction/forward
top level: Ph-s
start: 81
end: 101
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976425
display id: annotation1976425
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
type: Range
persistent identity: range1976425
display id: range1976425
version: 1
direction: direction/forward
top level: Ph-s
start: 69
end: 74
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976424
display id: annotation1976424
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
type: Range
persistent identity: range1976424
display id: range1976424
version: 1
direction: direction/forward
top level: Ph-s
start: 46
end: 50
orientation: inline

BBa_K823007
type: ComponentDefinition
persistent identity: BBa K823007
display id: BBa_K823007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23103
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:46:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: J23103
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823007_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182517
display id: annotation2182517
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1
type: Range
persistent identity: range2182517
display id: range2182517
version: 1
direction: direction/forward
top level: BBa_K823007
start: 24
end: 29
orientation: inline

J23103
type: SequenceAnnotation
persistent identity: annotation2182515
display id: annotation2182515
version: 1
top level: BBa_K823007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1
type: Range
persistent identity: range2182515
display id: range2182515
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182516
display id: annotation2182516
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1
type: Range
persistent identity: range2182516
display id: range2182516
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 6
orientation: inline