//chassis/prokaryote/mflorum
type: Collection
persistent identity: chassis prokaryote mflorum
display id: chassis_prokaryote_mflorum
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: gmR
member: BBa_J70007
member: BBa_J70304
member: BBa_J70502
member: BBa_J70002
member: BBa_J70300
member: BBa_J70510
member: BBa_J70028
member: BBa_M1000
member: BBa_J70024
member: BBa_J70508
member: BBa_J70635
member: BBa_J70665
member: BBa_J70025
member: BBa_J70506
member: BBa_J70301
member: BBa_J70512
member: BBa_J70666
member: BBa_M1001
member: BBa_J70511
member: BBa_J70503
member: BBa_J70029
member: BBa_J70507
member: BBa_J70636
member: BBa_J70513
member: BBa_J70005
member: BBa_J70302
member: BBa_J70500
member: BBa_J70310
member: BBa_J70655
member: P43-spoVG
member: BBa_J70504
member: BBa_J70667
member: BBa_J70026
member: BBa_J70006
member: BBa_J70303
member: BBa_J70402
member: BBa_J70501
member: GmR
member: BBa_J70509
member: BBa_J70634
member: BBa_J70023
member: BBa_J70505
member: BBa_J70027

BBa_J70310
type: ComponentDefinition
persistent identity: BBa J70310
display id: BBa_J70310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum pBG7AU plasmid origin
creator: Tom Knight
created: 2009-06-15T11:00:00Z
modified: 2015-05-08T01:08:21Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a biobricked version of the circular plasmid pBG7AU, Genbank NC_002569. The part is cut at the end of the Orf1 coding region, following the transcriptional terminator. This is similar to the location of the cut site used in the pKMK1 construction of Dybvig et al (King94) which formed their plasmid p2D4.
mutable notes: Unclear location for the cut site of the circular plasmid.
mutable provenance: PCR from recircularized pBG7AU cut at the ClaI site. Original plasmid from Djordjevic01. Primers: GTTTCTTC GAATTC GCGGCCGC T TCTAGA Ggagtaaattctcctttttttgg,BG7AU-F GTTTCTTC CTGCAG CGGCCGC T ACTAGT Acaaaaaaaggagaatttactcc,BG7AU-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: orfB
sequence annotation: orfA
sequence annotation: terminator
sequence annotation: ClaI site
sequence: https://synbiohub.org/public/igem/BBa_J70310_sequence/1

orfB
type: SequenceAnnotation
persistent identity: annotation2005556
display id: annotation2005556
version: 1
top level: BBa_J70310
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70310/annotation2005556/range2005556/1

https://synbiohub.org/public/igem/BBa_J70310/annotation2005556/range2005556/1
type: Range
persistent identity: range2005556
display id: range2005556
version: 1
direction: direction/forward
top level: BBa_J70310
start: 196
end: 354
orientation: inline

orfA
type: SequenceAnnotation
persistent identity: annotation2005557
display id: annotation2005557
version: 1
top level: BBa_J70310
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70310/annotation2005557/range2005557/1

https://synbiohub.org/public/igem/BBa_J70310/annotation2005557/range2005557/1
type: Range
persistent identity: range2005557
display id: range2005557
version: 1
direction: direction/forward
top level: BBa_J70310
start: 414
end: 1019
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2005558
display id: annotation2005558
version: 1
top level: BBa_J70310
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70310/annotation2005558/range2005558/1

https://synbiohub.org/public/igem/BBa_J70310/annotation2005558/range2005558/1
type: Range
persistent identity: range2005558
display id: range2005558
version: 1
direction: direction/forward
top level: BBa_J70310
start: 1003
end: 1034
orientation: inline

ClaI site
type: SequenceAnnotation
persistent identity: annotation2011588
display id: annotation2011588
version: 1
top level: BBa_J70310
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70310/annotation2011588/range2011588/1

https://synbiohub.org/public/igem/BBa_J70310/annotation2011588/range2011588/1
type: Range
persistent identity: range2011588
display id: range2011588
version: 1
direction: direction/forward
top level: BBa_J70310
start: 800
end: 805
orientation: inline

BBa_J70634
type: ComponentDefinition
persistent identity: BBa J70634
display id: BBa_J70634
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment
creator: Tom Knight
created: 2010-11-01T12:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Approximately 450 bp upstream DNA fragment of M. florum genome from sites 352951-353409 intended as a recombination site for homologous recombination, removing the restriction enzyme and methylase from M. florum.
mutable notes: Avoidance of biobrick restriction sites, possible primer binding sites.
mutable provenance: PCR from the M. florum genome. Primers: * ttccgaattcgcggccgcttctagag tccaaatagtgtggatac,Mfl2I-up-F * ttccactagtaaagacatctatgtttttttac,Mfl2I-up-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70634_sequence/1

BBa_J70513
type: ComponentDefinition
persistent identity: BBa J70513
display id: BBa_J70513
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet resistance + Mesoplasma origin fragment
creator: Tom Knight
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet resistance + Mesoplasma origin fragment
mutable notes: None.
mutable provenance: Assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70024
component: BBa_J70025
component: BBa_J70510
sequence annotation: BBa_J70024
sequence annotation: BBa_J70025
sequence annotation: BBa_J70510
sequence: https://synbiohub.org/public/igem/BBa_J70513_sequence/1

BBa_J70024
type: Component
persistent identity: component2028120
display id: component2028120
version: 1
top level: BBa_J70513
access: public
definition: BBa_J70024

BBa_J70025
type: Component
persistent identity: component2028118
display id: component2028118
version: 1
top level: BBa_J70513
access: public
definition: BBa_J70025

BBa_J70510
type: Component
persistent identity: component2028121
display id: component2028121
version: 1
top level: BBa_J70513
access: public
definition: BBa_J70510

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2028120
display id: annotation2028120
version: 1
top level: BBa_J70513
location: https://synbiohub.org/public/igem/BBa_J70513/annotation2028120/range2028120/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70513/annotation2028120/range2028120/1
type: Range
persistent identity: range2028120
display id: range2028120
version: 1
direction: direction/reverse
top level: BBa_J70513
start: 352
end: 2271
orientation: inline

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2028118
display id: annotation2028118
version: 1
top level: BBa_J70513
location: https://synbiohub.org/public/igem/BBa_J70513/annotation2028118/range2028118/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70513/annotation2028118/range2028118/1
type: Range
persistent identity: range2028118
display id: range2028118
version: 1
direction: direction/reverse
top level: BBa_J70513
start: 1
end: 345
orientation: inline

BBa_J70510
type: SequenceAnnotation
persistent identity: annotation2028121
display id: annotation2028121
version: 1
top level: BBa_J70513
location: https://synbiohub.org/public/igem/BBa_J70513/annotation2028121/range2028121/1
component: BBa_J70510

https://synbiohub.org/public/igem/BBa_J70513/annotation2028121/range2028121/1
type: Range
persistent identity: range2028121
display id: range2028121
version: 1
direction: direction/reverse
top level: BBa_J70513
start: 2280
end: 2652
orientation: inline

BBa_J70635
type: ComponentDefinition
persistent identity: BBa J70635
display id: BBa_J70635
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Downstream genomic DNA fragment of Mesoplasma florum for recombination experiment
creator: Tom Knight
created: 2010-11-01T12:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Recombination site downstream of the restriction enzyme and methylase of M florum. 700 bp fragment between 356063-356763.
mutable notes: no biobrick restriction enzyme sitss, primer binding
mutable provenance: PCR from the M. florum genome. Primers: * ttccgaattcgcggccgcttctagag gagattattatggcaaaag,Mfl2I-dn-F * ttccactagtagtaatatgagtaaattcac,Mfl2I-dn-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70635_sequence/1

BBa_J70029
type: ComponentDefinition
persistent identity: BBa J70029
display id: BBa_J70029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + mCherry gene, optimized for expression in Me. florum and E. coli
creator: Tom Knight
created: 2009-03-27T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: true
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS + mCherry gene, optimized for expression in Me. florum and E. coli.
mutable notes: Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
mutable provenance: Synthesized by DNA 2.0, designed with Gene Designer. Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS
sequence annotation: MF-RED
sequence: https://synbiohub.org/public/igem/BBa_J70029_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2002220
display id: annotation2002220
version: 1
top level: BBa_J70029
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70029/annotation2002220/range2002220/1

https://synbiohub.org/public/igem/BBa_J70029/annotation2002220/range2002220/1
type: Range
persistent identity: range2002220
display id: range2002220
version: 1
direction: direction/forward
top level: BBa_J70029
start: 7
end: 13
orientation: inline

MF-RED
type: SequenceAnnotation
persistent identity: annotation2002219
display id: annotation2002219
version: 1
top level: BBa_J70029
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70029/annotation2002219/range2002219/1

https://synbiohub.org/public/igem/BBa_J70029/annotation2002219/range2002219/1
type: Range
persistent identity: range2002219
display id: range2002219
version: 1
direction: direction/forward
top level: BBa_J70029
start: 19
end: 699
orientation: inline

BBa_J70304
type: ComponentDefinition
persistent identity: BBa J70304
display id: BBa_J70304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive cat cassette for Mesoplasma florum
creator: Tom Knight
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct.
mutable notes: Test for KpnI sites in the source plasmid.
mutable provenance: PCR from pSB1T3-J70026 with primers GTTTCTTCggtacctttcttcattagaatcaatcc,cat-mut-R GTTTCTTCggtacctttaggaggcatatcaaatg,cat-mut-F These primers bind to the source plasmid and remove the left side of the hairpin and add a pair of KpnI sites, which will be cut and religated. I hope to select on Cm plates, since the construct should be Cm resistant.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: UP sequence
sequence annotation: KpnI site
sequence annotation: terminator
sequence annotation: rbs
sequence annotation: -10
sequence annotation: cat
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J70304_sequence/1

UP sequence
type: SequenceAnnotation
persistent identity: annotation2002619
display id: annotation2002619
version: 1
top level: BBa_J70304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002619/range2002619/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002619/range2002619/1
type: Range
persistent identity: range2002619
display id: range2002619
version: 1
direction: direction/forward
top level: BBa_J70304
start: 157
end: 179
orientation: inline

KpnI site
type: SequenceAnnotation
persistent identity: annotation2002625
display id: annotation2002625
version: 1
top level: BBa_J70304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002625/range2002625/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002625/range2002625/1
type: Range
persistent identity: range2002625
display id: range2002625
version: 1
direction: direction/forward
top level: BBa_J70304
start: 227
end: 233
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2002624
display id: annotation2002624
version: 1
top level: BBa_J70304
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002624/range2002624/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002624/range2002624/1
type: Range
persistent identity: range2002624
display id: range2002624
version: 1
direction: direction/forward
top level: BBa_J70304
start: 914
end: 936
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2002622
display id: annotation2002622
version: 1
top level: BBa_J70304
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002622/range2002622/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002622/range2002622/1
type: Range
persistent identity: range2002622
display id: range2002622
version: 1
direction: direction/forward
top level: BBa_J70304
start: 237
end: 241
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002621
display id: annotation2002621
version: 1
top level: BBa_J70304
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002621/range2002621/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002621/range2002621/1
type: Range
persistent identity: range2002621
display id: range2002621
version: 1
direction: direction/forward
top level: BBa_J70304
start: 215
end: 220
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2002623
display id: annotation2002623
version: 1
top level: BBa_J70304
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002623/range2002623/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002623/range2002623/1
type: Range
persistent identity: range2002623
display id: range2002623
version: 1
direction: direction/forward
top level: BBa_J70304
start: 251
end: 901
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002620
display id: annotation2002620
version: 1
top level: BBa_J70304
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70304/annotation2002620/range2002620/1

https://synbiohub.org/public/igem/BBa_J70304/annotation2002620/range2002620/1
type: Range
persistent identity: range2002620
display id: range2002620
version: 1
direction: direction/forward
top level: BBa_J70304
start: 192
end: 197
orientation: inline

BBa_J70667
type: ComponentDefinition
persistent identity: BBa J70667
display id: BBa_J70667
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MF optimized RFP reporter, MF heat induced promoter
creator: Tom Knight
created: 2011-04-04T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: J70301, J70655
mutable notes: None
mutable provenance: Composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J70301
component: BBa_J70655
sequence annotation: BBa_J70655
sequence annotation: BBa_J70301
sequence: https://synbiohub.org/public/igem/BBa_J70667_sequence/1

BBa_J70301
type: Component
persistent identity: component2115791
display id: component2115791
version: 1
top level: BBa_J70667
access: public
definition: BBa_J70301

BBa_J70655
type: Component
persistent identity: component2115793
display id: component2115793
version: 1
top level: BBa_J70667
access: public
definition: BBa_J70655

BBa_J70655
type: SequenceAnnotation
persistent identity: annotation2115793
display id: annotation2115793
version: 1
top level: BBa_J70667
location: https://synbiohub.org/public/igem/BBa_J70667/annotation2115793/range2115793/1
component: BBa_J70655

https://synbiohub.org/public/igem/BBa_J70667/annotation2115793/range2115793/1
type: Range
persistent identity: range2115793
display id: range2115793
version: 1
direction: direction/reverse
top level: BBa_J70667
start: 120
end: 818
orientation: inline

BBa_J70301
type: SequenceAnnotation
persistent identity: annotation2115791
display id: annotation2115791
version: 1
top level: BBa_J70667
location: https://synbiohub.org/public/igem/BBa_J70667/annotation2115791/range2115791/1
component: BBa_J70301

https://synbiohub.org/public/igem/BBa_J70667/annotation2115791/range2115791/1
type: Range
persistent identity: range2115791
display id: range2115791
version: 1
direction: direction/reverse
top level: BBa_J70667
start: 1
end: 111
orientation: inline

BBa_J70027
type: ComponentDefinition
persistent identity: BBa J70027
display id: BBa_J70027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum plasmid origin
creator: Tom Knight
created: 2008-11-04T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein.
mutable notes: No native restriction sites. Added biobrick ends. The gene for PE contains no CGG codons. GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R The start of J70027 matches the origin start in the pSci1 plasmid.
mutable provenance: Genbank AJ969070
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: J70023-F2
sequence annotation: dnaA binding
sequence annotation: imperfect repeat
sequence annotation: PE
sequence annotation: Low GC region
sequence annotation: J70023-R
sequence annotation: RBS
sequence annotation: iteron
sequence: https://synbiohub.org/public/igem/BBa_J70027_sequence/1

J70023-F2
type: SequenceAnnotation
persistent identity: annotation1999311
display id: annotation1999311
version: 1
top level: BBa_J70027
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999311/range1999311/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999311/range1999311/1
type: Range
persistent identity: range1999311
display id: range1999311
version: 1
direction: direction/forward
top level: BBa_J70027
start: 1
end: 25
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999312
display id: annotation1999312
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999312/range1999312/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999312/range1999312/1
type: Range
persistent identity: range1999312
display id: range1999312
version: 1
direction: direction/forward
top level: BBa_J70027
start: 152
end: 160
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999317
display id: annotation1999317
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999317/range1999317/1

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type: Range
persistent identity: range1999317
display id: range1999317
version: 1
direction: direction/reverse
top level: BBa_J70027
start: 587
end: 595
orientation: inline

imperfect repeat
type: SequenceAnnotation
persistent identity: annotation1999314
display id: annotation1999314
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999314/range1999314/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999314/range1999314/1
type: Range
persistent identity: range1999314
display id: range1999314
version: 1
direction: direction/forward
top level: BBa_J70027
start: 282
end: 293
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999313
display id: annotation1999313
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999313/range1999313/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999313/range1999313/1
type: Range
persistent identity: range1999313
display id: range1999313
version: 1
direction: direction/reverse
top level: BBa_J70027
start: 218
end: 226
orientation: inline

PE
type: SequenceAnnotation
persistent identity: annotation1999318
display id: annotation1999318
version: 1
top level: BBa_J70027
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999318/range1999318/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999318/range1999318/1
type: Range
persistent identity: range1999318
display id: range1999318
version: 1
direction: direction/forward
top level: BBa_J70027
start: 654
end: 1307
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1999338
display id: annotation1999338
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999338/range1999338/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999338/range1999338/1
type: Range
persistent identity: range1999338
display id: range1999338
version: 1
direction: direction/forward
top level: BBa_J70027
start: 1470
end: 1646
orientation: inline

J70023-R
type: SequenceAnnotation
persistent identity: annotation1999339
display id: annotation1999339
version: 1
top level: BBa_J70027
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999339/range1999339/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999339/range1999339/1
type: Range
persistent identity: range1999339
display id: range1999339
version: 1
direction: direction/reverse
top level: BBa_J70027
start: 1678
end: 1701
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1999319
display id: annotation1999319
version: 1
top level: BBa_J70027
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999319/range1999319/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999319/range1999319/1
type: Range
persistent identity: range1999319
display id: range1999319
version: 1
direction: direction/forward
top level: BBa_J70027
start: 640
end: 644
orientation: inline

iteron
type: SequenceAnnotation
persistent identity: annotation1999320
display id: annotation1999320
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999320/range1999320/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999320/range1999320/1
type: Range
persistent identity: range1999320
display id: range1999320
version: 1
direction: direction/forward
top level: BBa_J70027
start: 1305
end: 1315
orientation: inline

imperfect repeat
type: SequenceAnnotation
persistent identity: annotation1999316
display id: annotation1999316
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999316/range1999316/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999316/range1999316/1
type: Range
persistent identity: range1999316
display id: range1999316
version: 1
direction: direction/forward
top level: BBa_J70027
start: 403
end: 413
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999315
display id: annotation1999315
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999315/range1999315/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999315/range1999315/1
type: Range
persistent identity: range1999315
display id: range1999315
version: 1
direction: direction/reverse
top level: BBa_J70027
start: 296
end: 304
orientation: inline

iteron
type: SequenceAnnotation
persistent identity: annotation1999321
display id: annotation1999321
version: 1
top level: BBa_J70027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70027/annotation1999321/range1999321/1

https://synbiohub.org/public/igem/BBa_J70027/annotation1999321/range1999321/1
type: Range
persistent identity: range1999321
display id: range1999321
version: 1
direction: direction/forward
top level: BBa_J70027
start: 1325
end: 1335
orientation: inline

BBa_J70501
type: ComponentDefinition
persistent identity: BBa J70501
display id: BBa_J70501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid origin from pWV01 + Tet resistance gene for plasmid construction
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid origin from pWV01 + Tet resistance gene for plasmid construction. Intended to be cut with XS and inserted into the NheI sites of vector construction part I15020.
mutable notes: Avoid assembly into tet resistant plasmids since it already has tet resistance.
mutable provenance: Assembly into pSB1C3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70500
component: BBa_J70024
component: BBa_J70025
sequence annotation: BBa_J70500
sequence annotation: BBa_J70024
sequence annotation: BBa_J70025
sequence: https://synbiohub.org/public/igem/BBa_J70501_sequence/1

BBa_J70500
type: Component
persistent identity: component2027947
display id: component2027947
version: 1
top level: BBa_J70501
access: public
definition: BBa_J70500

BBa_J70024
type: Component
persistent identity: component2027960
display id: component2027960
version: 1
top level: BBa_J70501
access: public
definition: BBa_J70024

BBa_J70025
type: Component
persistent identity: component2027958
display id: component2027958
version: 1
top level: BBa_J70501
access: public
definition: BBa_J70025

BBa_J70500
type: SequenceAnnotation
persistent identity: annotation2027947
display id: annotation2027947
version: 1
top level: BBa_J70501
location: https://synbiohub.org/public/igem/BBa_J70501/annotation2027947/range2027947/1
component: BBa_J70500

https://synbiohub.org/public/igem/BBa_J70501/annotation2027947/range2027947/1
type: Range
persistent identity: range2027947
display id: range2027947
version: 1
direction: direction/reverse
top level: BBa_J70501
start: 1
end: 1747
orientation: inline

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2027960
display id: annotation2027960
version: 1
top level: BBa_J70501
location: https://synbiohub.org/public/igem/BBa_J70501/annotation2027960/range2027960/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70501/annotation2027960/range2027960/1
type: Range
persistent identity: range2027960
display id: range2027960
version: 1
direction: direction/reverse
top level: BBa_J70501
start: 2107
end: 4026
orientation: inline

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2027958
display id: annotation2027958
version: 1
top level: BBa_J70501
location: https://synbiohub.org/public/igem/BBa_J70501/annotation2027958/range2027958/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70501/annotation2027958/range2027958/1
type: Range
persistent identity: range2027958
display id: range2027958
version: 1
direction: direction/reverse
top level: BBa_J70501
start: 1756
end: 2100
orientation: inline

BBa_J70655
type: ComponentDefinition
persistent identity: BBa J70655
display id: BBa_J70655
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP optimized for expression in E. coli and M. florum
creator: Tom Knight
created: 2010-12-01T12:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codon optimized version of red fluorescent protein, optimized for dual expression in E. coli and M. florum. Includes an RBS and CDS. Designed to express well in either species. Designed with Gene Designer and made by DNA 2.0, provided in their pJ201 plasmid with Kan resistance.
mutable notes: Restriction site elimination, coding bias, elimination of CGG codon.
mutable provenance: Design, DNA2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: MF-RED
sequence: https://synbiohub.org/public/igem/BBa_J70655_sequence/1

MF-RED
type: SequenceAnnotation
persistent identity: annotation2114854
display id: annotation2114854
version: 1
top level: BBa_J70655
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70655/annotation2114854/range2114854/1

https://synbiohub.org/public/igem/BBa_J70655/annotation2114854/range2114854/1
type: Range
persistent identity: range2114854
display id: range2114854
version: 1
direction: direction/forward
top level: BBa_J70655
start: 52
end: 699
orientation: inline

BBa_J70502
type: ComponentDefinition
persistent identity: BBa J70502
display id: BBa_J70502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid origin from pWV01 + Tet resistance gene for plasmid construction
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid origin from pWV01 + Tet resistance gene for plasmid construction. Intended to be cut with XS and inserted into the NheI sites of vector construction part I15020.
mutable notes: Assembly into pSB1C3
mutable provenance: Avoid assembly into tet resistant plasmids since it already has tet resistance.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70500
component: BBa_J70025
component: BBa_J70024
sequence annotation: BBa_J70024
sequence annotation: BBa_J70025
sequence annotation: BBa_J70500
sequence: https://synbiohub.org/public/igem/BBa_J70502_sequence/1

BBa_J70500
type: Component
persistent identity: component2027988
display id: component2027988
version: 1
top level: BBa_J70502
access: public
definition: BBa_J70500

BBa_J70025
type: Component
persistent identity: component2027973
display id: component2027973
version: 1
top level: BBa_J70502
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component2027975
display id: component2027975
version: 1
top level: BBa_J70502
access: public
definition: BBa_J70024

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2027975
display id: annotation2027975
version: 1
top level: BBa_J70502
location: https://synbiohub.org/public/igem/BBa_J70502/annotation2027975/range2027975/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70502/annotation2027975/range2027975/1
type: Range
persistent identity: range2027975
display id: range2027975
version: 1
direction: direction/reverse
top level: BBa_J70502
start: 352
end: 2271
orientation: inline

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2027973
display id: annotation2027973
version: 1
top level: BBa_J70502
location: https://synbiohub.org/public/igem/BBa_J70502/annotation2027973/range2027973/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70502/annotation2027973/range2027973/1
type: Range
persistent identity: range2027973
display id: range2027973
version: 1
direction: direction/reverse
top level: BBa_J70502
start: 1
end: 345
orientation: inline

BBa_J70500
type: SequenceAnnotation
persistent identity: annotation2027988
display id: annotation2027988
version: 1
top level: BBa_J70502
location: https://synbiohub.org/public/igem/BBa_J70502/annotation2027988/range2027988/1
component: BBa_J70500

https://synbiohub.org/public/igem/BBa_J70502/annotation2027988/range2027988/1
type: Range
persistent identity: range2027988
display id: range2027988
version: 1
direction: direction/reverse
top level: BBa_J70502
start: 2280
end: 4026
orientation: inline

BBa_J70511
type: ComponentDefinition
persistent identity: BBa J70511
display id: BBa_J70511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet resistance + Mesoplasma origin fragment
creator: Tom Knight
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet resistance + Mesoplasma origin fragment
mutable notes: None.
mutable provenance: Assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70025
component: BBa_J70024
component: BBa_J70504
sequence annotation: BBa_J70024
sequence annotation: BBa_J70025
sequence annotation: BBa_J70504
sequence: https://synbiohub.org/public/igem/BBa_J70511_sequence/1

BBa_J70025
type: Component
persistent identity: component2028086
display id: component2028086
version: 1
top level: BBa_J70511
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component2028088
display id: component2028088
version: 1
top level: BBa_J70511
access: public
definition: BBa_J70024

BBa_J70504
type: Component
persistent identity: component2028089
display id: component2028089
version: 1
top level: BBa_J70511
access: public
definition: BBa_J70504

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2028088
display id: annotation2028088
version: 1
top level: BBa_J70511
location: https://synbiohub.org/public/igem/BBa_J70511/annotation2028088/range2028088/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70511/annotation2028088/range2028088/1
type: Range
persistent identity: range2028088
display id: range2028088
version: 1
direction: direction/reverse
top level: BBa_J70511
start: 352
end: 2271
orientation: inline

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2028086
display id: annotation2028086
version: 1
top level: BBa_J70511
location: https://synbiohub.org/public/igem/BBa_J70511/annotation2028086/range2028086/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70511/annotation2028086/range2028086/1
type: Range
persistent identity: range2028086
display id: range2028086
version: 1
direction: direction/reverse
top level: BBa_J70511
start: 1
end: 345
orientation: inline

BBa_J70504
type: SequenceAnnotation
persistent identity: annotation2028089
display id: annotation2028089
version: 1
top level: BBa_J70511
location: https://synbiohub.org/public/igem/BBa_J70511/annotation2028089/range2028089/1
component: BBa_J70504

https://synbiohub.org/public/igem/BBa_J70511/annotation2028089/range2028089/1
type: Range
persistent identity: range2028089
display id: range2028089
version: 1
direction: direction/reverse
top level: BBa_J70511
start: 2280
end: 4260
orientation: inline

BBa_J70504
type: ComponentDefinition
persistent identity: BBa J70504
display id: BBa_J70504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F2 to OR-R4 GTTTCTTC T TCTAGA G ttttgatggttgtcaagttc,OF-F2 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A cctgtaatgctgggttaac,OR-R4
mutable notes: PCR from genomic DNA
mutable provenance: Needs mutation of EcoRI sites and GATC sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70504_sequence/1

BBa_J70007
type: ComponentDefinition
persistent identity: BBa J70007
display id: BBa_J70007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recT recombination gene from Spiroplasma citri
creator: Tom Knight
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: recET recombination gene from Spiroplasma citri, probably catalyzing the homologous recombination of DNA fragments similar to the Lambda Bet gene (Lambda RED recombination). This gene is from S. citri, a close relative of Me. florum, and has very similar codon usage.
mutable notes: Eliminate GATC site by silent mutation to GACC (asp -> asp). Note very low GC content at the 5' end. The primers are: * gtttcttcgaattcgcggccgcttctagatgaataataaaattgaattaatattaaataattc,recT-F * gtttcttctactagtagcggccgctgcagttattaatcagaaaacatttcattaacagc,recT-R * gaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagc,recT-mut-F * gctgttattaataattattgcttggtcatgttgataggcttcttgcatttc,recT-mut-R
mutable provenance: Genbank entry CAK99381 protein, nucleotide coding region. Mutations of the single GATC site to avoid restriction by the Sau3AI restriction system in Me. florum. PCR from S. citri DNA, kind gift of Prof. J. Renaudin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: recT
sequence: https://synbiohub.org/public/igem/BBa_J70007_sequence/1

recT
type: SequenceAnnotation
persistent identity: annotation1983023
display id: annotation1983023
version: 1
top level: BBa_J70007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70007/annotation1983023/range1983023/1

https://synbiohub.org/public/igem/BBa_J70007/annotation1983023/range1983023/1
type: Range
persistent identity: range1983023
display id: range1983023
version: 1
direction: direction/forward
top level: BBa_J70007
start: 1
end: 858
orientation: inline

BBa_J70023
type: ComponentDefinition
persistent identity: BBa J70023
display id: BBa_J70023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MF-plasmid-ori
creator: Tom Knight
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein.
mutable notes: No native restriction sites. Added biobrick ends.
mutable provenance: Genbank AJ969070
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: dnaA binding
sequence annotation: Iteron
sequence annotation: pe
sequence annotation: Low GC region
sequence annotation: fwd2
sequence annotation: imperfect repeat
sequence annotation: reverse PCR primer
sequence annotation: forward PCR priimer
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J70023_sequence/1

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999325
display id: annotation1999325
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999325/range1999325/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999325/range1999325/1
type: Range
persistent identity: range1999325
display id: range1999325
version: 1
direction: direction/forward
top level: BBa_J70023
start: 328
end: 336
orientation: inline

Iteron
type: SequenceAnnotation
persistent identity: annotation1999333
display id: annotation1999333
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999333/range1999333/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999333/range1999333/1
type: Range
persistent identity: range1999333
display id: range1999333
version: 1
direction: direction/forward
top level: BBa_J70023
start: 1481
end: 1491
orientation: inline

pe
type: SequenceAnnotation
persistent identity: annotation1999332
display id: annotation1999332
version: 1
top level: BBa_J70023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999332/range1999332/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999332/range1999332/1
type: Range
persistent identity: range1999332
display id: range1999332
version: 1
direction: direction/forward
top level: BBa_J70023
start: 830
end: 1483
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1999335
display id: annotation1999335
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999335/range1999335/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999335/range1999335/1
type: Range
persistent identity: range1999335
display id: range1999335
version: 1
direction: direction/forward
top level: BBa_J70023
start: 1646
end: 1822
orientation: inline

fwd2
type: SequenceAnnotation
persistent identity: annotation1999324
display id: annotation1999324
version: 1
top level: BBa_J70023
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999324/range1999324/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999324/range1999324/1
type: Range
persistent identity: range1999324
display id: range1999324
version: 1
direction: direction/forward
top level: BBa_J70023
start: 177
end: 201
orientation: inline

imperfect repeat
type: SequenceAnnotation
persistent identity: annotation1999327
display id: annotation1999327
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999327/range1999327/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999327/range1999327/1
type: Range
persistent identity: range1999327
display id: range1999327
version: 1
direction: direction/forward
top level: BBa_J70023
start: 458
end: 469
orientation: inline

Iteron
type: SequenceAnnotation
persistent identity: annotation1999334
display id: annotation1999334
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999334/range1999334/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999334/range1999334/1
type: Range
persistent identity: range1999334
display id: range1999334
version: 1
direction: direction/forward
top level: BBa_J70023
start: 1501
end: 1511
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999328
display id: annotation1999328
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999328/range1999328/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999328/range1999328/1
type: Range
persistent identity: range1999328
display id: range1999328
version: 1
direction: direction/reverse
top level: BBa_J70023
start: 472
end: 480
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999326
display id: annotation1999326
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999326/range1999326/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999326/range1999326/1
type: Range
persistent identity: range1999326
display id: range1999326
version: 1
direction: direction/reverse
top level: BBa_J70023
start: 394
end: 402
orientation: inline

imperfect repeat
type: SequenceAnnotation
persistent identity: annotation1999329
display id: annotation1999329
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999329/range1999329/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999329/range1999329/1
type: Range
persistent identity: range1999329
display id: range1999329
version: 1
direction: direction/forward
top level: BBa_J70023
start: 579
end: 589
orientation: inline

reverse PCR primer
type: SequenceAnnotation
persistent identity: annotation1999336
display id: annotation1999336
version: 1
top level: BBa_J70023
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999336/range1999336/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999336/range1999336/1
type: Range
persistent identity: range1999336
display id: range1999336
version: 1
direction: direction/reverse
top level: BBa_J70023
start: 1854
end: 1877
orientation: inline

forward PCR priimer
type: SequenceAnnotation
persistent identity: annotation1999323
display id: annotation1999323
version: 1
top level: BBa_J70023
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999323/range1999323/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999323/range1999323/1
type: Range
persistent identity: range1999323
display id: range1999323
version: 1
direction: direction/forward
top level: BBa_J70023
start: 1
end: 27
orientation: inline

dnaA binding
type: SequenceAnnotation
persistent identity: annotation1999330
display id: annotation1999330
version: 1
top level: BBa_J70023
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999330/range1999330/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999330/range1999330/1
type: Range
persistent identity: range1999330
display id: range1999330
version: 1
direction: direction/reverse
top level: BBa_J70023
start: 763
end: 771
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1999331
display id: annotation1999331
version: 1
top level: BBa_J70023
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70023/annotation1999331/range1999331/1

https://synbiohub.org/public/igem/BBa_J70023/annotation1999331/range1999331/1
type: Range
persistent identity: range1999331
display id: range1999331
version: 1
direction: direction/forward
top level: BBa_J70023
start: 816
end: 820
orientation: inline

BBa_J70302
type: ComponentDefinition
persistent identity: BBa J70302
display id: BBa_J70302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test plasmid for Mesoplasma florum
creator: Tom Knight
created: 2009-04-17T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Assembly of tetM cassette and pSCI2 plasmid origin into E. coli plasmid pSB1K3.
mutable notes: Assembled into Kan resistant part from cm resistant parts. Avoid tet resistant backbone plasmids, since the insert is tet resistant.
mutable provenance: Assembly from two registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70025
component: BBa_J70024
component: BBa_J70027
sequence annotation: BBa_J70025
sequence annotation: BBa_J70027
sequence annotation: BBa_J70024
sequence: https://synbiohub.org/public/igem/BBa_J70302_sequence/1

BBa_J70025
type: Component
persistent identity: component2002422
display id: component2002422
version: 1
top level: BBa_J70302
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component2002424
display id: component2002424
version: 1
top level: BBa_J70302
access: public
definition: BBa_J70024

BBa_J70027
type: Component
persistent identity: component2002411
display id: component2002411
version: 1
top level: BBa_J70302
access: public
definition: BBa_J70027

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2002422
display id: annotation2002422
version: 1
top level: BBa_J70302
location: https://synbiohub.org/public/igem/BBa_J70302/annotation2002422/range2002422/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70302/annotation2002422/range2002422/1
type: Range
persistent identity: range2002422
display id: range2002422
version: 1
direction: direction/reverse
top level: BBa_J70302
start: 1710
end: 2054
orientation: inline

BBa_J70027
type: SequenceAnnotation
persistent identity: annotation2002411
display id: annotation2002411
version: 1
top level: BBa_J70302
location: https://synbiohub.org/public/igem/BBa_J70302/annotation2002411/range2002411/1
component: BBa_J70027

https://synbiohub.org/public/igem/BBa_J70302/annotation2002411/range2002411/1
type: Range
persistent identity: range2002411
display id: range2002411
version: 1
direction: direction/reverse
top level: BBa_J70302
start: 1
end: 1701
orientation: inline

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2002424
display id: annotation2002424
version: 1
top level: BBa_J70302
location: https://synbiohub.org/public/igem/BBa_J70302/annotation2002424/range2002424/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70302/annotation2002424/range2002424/1
type: Range
persistent identity: range2002424
display id: range2002424
version: 1
direction: direction/reverse
top level: BBa_J70302
start: 2061
end: 3980
orientation: inline

BBa_J70636
type: ComponentDefinition
persistent identity: BBa J70636
display id: BBa_J70636
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pheS gene from M. florum
creator: Tom Knight
created: 2010-11-01T12:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pheS gene from the phenylalanine tRNA charging complex in M. florum, preparing for mutation for use with p-chlorophenylalanine counter selection (Kast, pKSS).
mutable notes: none.
mutable provenance: PCR from M. florum genomic DNA. Primers: * ttccgaattcgcggccgcttctagag ctctgttattggtatagtta,pheS-mf-F2 * ttccactagtatgttctacctactctcc,pheS-mf-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: pheS
sequence: https://synbiohub.org/public/igem/BBa_J70636_sequence/1

pheS
type: SequenceAnnotation
persistent identity: annotation2113724
display id: annotation2113724
version: 1
top level: BBa_J70636
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70636/annotation2113724/range2113724/1

https://synbiohub.org/public/igem/BBa_J70636/annotation2113724/range2113724/1
type: Range
persistent identity: range2113724
display id: range2113724
version: 1
direction: direction/forward
top level: BBa_J70636
start: 94
end: 1146
orientation: inline

BBa_J70402
type: ComponentDefinition
persistent identity: BBa J70402
display id: BBa_J70402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBG7AU (J70310) plus TetM (J70028)
creator: Kelly Drinkwater
created: 2009-07-27T11:00:00Z
modified: 2015-05-08T01:08:22Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 5347
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid pBG7AU (J70310) for Mesoplasma florum combined with Tet resistance cassette (J70028).
mutable notes: See J70310
mutable provenance: Constructed in Knight Lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70025
component: BBa_J70024
component: BBa_J70310
sequence annotation: BBa_J70024
sequence annotation: BBa_J70025
sequence annotation: BBa_J70310
sequence: https://synbiohub.org/public/igem/BBa_J70402_sequence/1

BBa_J70025
type: Component
persistent identity: component2013825
display id: component2013825
version: 1
top level: BBa_J70402
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component2013827
display id: component2013827
version: 1
top level: BBa_J70402
access: public
definition: BBa_J70024

BBa_J70310
type: Component
persistent identity: component2013814
display id: component2013814
version: 1
top level: BBa_J70402
access: public
definition: BBa_J70310

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2013827
display id: annotation2013827
version: 1
top level: BBa_J70402
location: https://synbiohub.org/public/igem/BBa_J70402/annotation2013827/range2013827/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70402/annotation2013827/range2013827/1
type: Range
persistent identity: range2013827
display id: range2013827
version: 1
direction: direction/reverse
top level: BBa_J70402
start: 1394
end: 3313
orientation: inline

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2013825
display id: annotation2013825
version: 1
top level: BBa_J70402
location: https://synbiohub.org/public/igem/BBa_J70402/annotation2013825/range2013825/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70402/annotation2013825/range2013825/1
type: Range
persistent identity: range2013825
display id: range2013825
version: 1
direction: direction/reverse
top level: BBa_J70402
start: 1043
end: 1387
orientation: inline

BBa_J70310
type: SequenceAnnotation
persistent identity: annotation2013814
display id: annotation2013814
version: 1
top level: BBa_J70402
location: https://synbiohub.org/public/igem/BBa_J70402/annotation2013814/range2013814/1
component: BBa_J70310

https://synbiohub.org/public/igem/BBa_J70402/annotation2013814/range2013814/1
type: Range
persistent identity: range2013814
display id: range2013814
version: 1
direction: direction/reverse
top level: BBa_J70402
start: 1
end: 1034
orientation: inline

BBa_J70506
type: ComponentDefinition
persistent identity: BBa J70506
display id: BBa_J70506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F1 to OR-R4 GTTTCTTC T TCTAGA G aagtgctggaaaaagtatag,OR-F1 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A cctgtaatgctgggttaac,OR-R4
mutable notes: PCR from genomic DNA
mutable provenance: Needs mutation of EcoRI sites and GATC sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70506_sequence/1

BBa_J70666
type: ComponentDefinition
persistent identity: BBa J70666
display id: BBa_J70666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MF optimized RFP reporter, MF promoter
creator: Tom Knight
created: 2011-04-04T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: J70300, J70655
mutable notes: None
mutable provenance: Composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J70655
component: BBa_J70300
sequence annotation: BBa_J70655
sequence annotation: BBa_J70300
sequence: https://synbiohub.org/public/igem/BBa_J70666_sequence/1

BBa_J70655
type: Component
persistent identity: component2115787
display id: component2115787
version: 1
top level: BBa_J70666
access: public
definition: BBa_J70655

BBa_J70300
type: Component
persistent identity: component2115785
display id: component2115785
version: 1
top level: BBa_J70666
access: public
definition: BBa_J70300

BBa_J70655
type: SequenceAnnotation
persistent identity: annotation2115787
display id: annotation2115787
version: 1
top level: BBa_J70666
location: https://synbiohub.org/public/igem/BBa_J70666/annotation2115787/range2115787/1
component: BBa_J70655

https://synbiohub.org/public/igem/BBa_J70666/annotation2115787/range2115787/1
type: Range
persistent identity: range2115787
display id: range2115787
version: 1
direction: direction/reverse
top level: BBa_J70666
start: 91
end: 789
orientation: inline

BBa_J70300
type: SequenceAnnotation
persistent identity: annotation2115785
display id: annotation2115785
version: 1
top level: BBa_J70666
location: https://synbiohub.org/public/igem/BBa_J70666/annotation2115785/range2115785/1
component: BBa_J70300

https://synbiohub.org/public/igem/BBa_J70666/annotation2115785/range2115785/1
type: Range
persistent identity: range2115785
display id: range2115785
version: 1
direction: direction/reverse
top level: BBa_J70666
start: 1
end: 82
orientation: inline

P43-spoVG
type: ComponentDefinition
persistent identity: BBa K143051
display id: BBa_K143051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter 43 and RBS spoVG for B. subtilis
creator: Chris Hirst
created: 2008-09-29T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. P43-spoVG can be used in the context of a Polymerase per second (PoPS) output generator. '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
mutable notes: The sequence of P43 and RBS-spoVG were obtained from papers1 2 and the sequence synthesised by GeneArt.
mutable provenance: P43-spoVG was synthesised by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143013
component: BBa_K143021
sequence annotation: BBa_K143013
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143051_sequence/1

BBa_K143013
type: Component
persistent identity: component1977514
display id: component1977514
version: 1
top level: P43-spoVG
access: public
definition: https://synbiohub.org/public/igem/BBa_K143013/1

BBa_K143021
type: Component
persistent identity: component1977516
display id: component1977516
version: 1
top level: P43-spoVG
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K143013
type: SequenceAnnotation
persistent identity: annotation1977514
display id: annotation1977514
version: 1
top level: P43-spoVG
location: https://synbiohub.org/public/igem/BBa_K143051/annotation1977514/range1977514/1
component: BBa_K143013

https://synbiohub.org/public/igem/BBa_K143051/annotation1977514/range1977514/1
type: Range
persistent identity: range1977514
display id: range1977514
version: 1
direction: direction/reverse
top level: P43-spoVG
start: 1
end: 56
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1977516
display id: annotation1977516
version: 1
top level: P43-spoVG
location: https://synbiohub.org/public/igem/BBa_K143051/annotation1977516/range1977516/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143051/annotation1977516/range1977516/1
type: Range
persistent identity: range1977516
display id: range1977516
version: 1
direction: direction/reverse
top level: P43-spoVG
start: 65
end: 76
orientation: inline

BBa_J70006
type: ComponentDefinition
persistent identity: BBa J70006
display id: BBa_J70006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Click Beetle Luciferase Green
creator: Spenser Skates
created: 2008-03-28T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 2541
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Click beetle luciferase derived from Promega's red/green system, modified using the E. coli/Mesoplasma florum compromise codon table on open wet ware. Removal of all restriction sites necessary to make fully Biobrick compliant.
mutable notes: -
mutable provenance: Derived from Promega's click beetle luciferase red/green system, originally designed to function in mammalian cells. From the open reading frame of the pCBG99 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J70006_sequence/1

BBa_J70002
type: ComponentDefinition
persistent identity: BBa J70002
display id: BBa_J70002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetM recoded for dual E. coli/Mesoplasma expression
creator: Tom Knight
created: 2007-08-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: true
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 6
owning group id: 48
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: TetM recoded for dual E. coli/Mesoplasma expression
mutable notes: codon usage
mutable provenance: Geneart synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J70002_sequence/1

BBa_J70507
type: ComponentDefinition
persistent identity: BBa J70507
display id: BBa_J70507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F2 to OR-R3 GTTTCTTC T TCTAGA G ttttgatggttgtcaagttc,OF-F2 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTATTAATTTTTCCTTCTTTAAC,OR-R2
mutable notes: None.
mutable provenance: PCR from genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70507_sequence/1

BBa_M1001
type: ComponentDefinition
persistent identity: BBa M1001
display id: BBa_M1001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right mosaic end for Tn5 transpososome
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _12_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Right end of a linear transpososome sequence containing the 19 bp mosaic end in the reverse orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1000 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
mutable provenance: Synthesis from overlapped oligos with PCR.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PvuII cut site
sequence annotation: Mosaic end (inverted)
sequence: https://synbiohub.org/public/igem/BBa_M1001_sequence/1

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825560
display id: annotation1825560
version: 1
top level: BBa_M1001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1001/annotation1825560/range1825560/1

https://synbiohub.org/public/igem/BBa_M1001/annotation1825560/range1825560/1
type: Range
persistent identity: range1825560
display id: range1825560
version: 1
direction: direction/forward
top level: BBa_M1001
start: 17
end: 22
orientation: inline

Mosaic end (inverted)
type: SequenceAnnotation
persistent identity: annotation1825561
display id: annotation1825561
version: 1
top level: BBa_M1001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1001/annotation1825561/range1825561/1

https://synbiohub.org/public/igem/BBa_M1001/annotation1825561/range1825561/1
type: Range
persistent identity: range1825561
display id: range1825561
version: 1
direction: direction/forward
top level: BBa_M1001
start: 1
end: 19
orientation: inline

BBa_J70025
type: ComponentDefinition
persistent identity: BBa J70025
display id: BBa_J70025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1
creator: Tom Knight
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024.
mutable notes: Alignment of the promoter with the coding region, preservation of the RBS sites.
mutable provenance: pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: +1
sequence annotation: stem_loop
sequence annotation: leader peptide
sequence annotation: RBS
sequence annotation: -10
sequence annotation: rbs
sequence annotation: term
sequence: https://synbiohub.org/public/igem/BBa_J70025_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1999389
display id: annotation1999389
version: 1
top level: BBa_J70025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999389/range1999389/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999389/range1999389/1
type: Range
persistent identity: range1999389
display id: range1999389
version: 1
direction: direction/forward
top level: BBa_J70025
start: 45
end: 50
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1999390
display id: annotation1999390
version: 1
top level: BBa_J70025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999390/range1999390/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999390/range1999390/1
type: Range
persistent identity: range1999390
display id: range1999390
version: 1
direction: direction/forward
top level: BBa_J70025
start: 80
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1999386
display id: annotation1999386
version: 1
top level: BBa_J70025
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999386/range1999386/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999386/range1999386/1
type: Range
persistent identity: range1999386
display id: range1999386
version: 1
direction: direction/forward
top level: BBa_J70025
start: 242
end: 272
orientation: inline

leader peptide
type: SequenceAnnotation
persistent identity: annotation1999391
display id: annotation1999391
version: 1
top level: BBa_J70025
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999391/range1999391/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999391/range1999391/1
type: Range
persistent identity: range1999391
display id: range1999391
version: 1
direction: direction/forward
top level: BBa_J70025
start: 250
end: 336
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1999382
display id: annotation1999382
version: 1
top level: BBa_J70025
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999382/range1999382/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999382/range1999382/1
type: Range
persistent identity: range1999382
display id: range1999382
version: 1
direction: direction/forward
top level: BBa_J70025
start: 339
end: 345
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1999384
display id: annotation1999384
version: 1
top level: BBa_J70025
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999384/range1999384/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999384/range1999384/1
type: Range
persistent identity: range1999384
display id: range1999384
version: 1
direction: direction/forward
top level: BBa_J70025
start: 273
end: 320
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1999385
display id: annotation1999385
version: 1
top level: BBa_J70025
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999385/range1999385/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999385/range1999385/1
type: Range
persistent identity: range1999385
display id: range1999385
version: 1
direction: direction/forward
top level: BBa_J70025
start: 254
end: 281
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1999388
display id: annotation1999388
version: 1
top level: BBa_J70025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999388/range1999388/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999388/range1999388/1
type: Range
persistent identity: range1999388
display id: range1999388
version: 1
direction: direction/forward
top level: BBa_J70025
start: 68
end: 73
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1999387
display id: annotation1999387
version: 1
top level: BBa_J70025
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999387/range1999387/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999387/range1999387/1
type: Range
persistent identity: range1999387
display id: range1999387
version: 1
direction: direction/forward
top level: BBa_J70025
start: 237
end: 240
orientation: inline

term
type: SequenceAnnotation
persistent identity: annotation1999383
display id: annotation1999383
version: 1
top level: BBa_J70025
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70025/annotation1999383/range1999383/1

https://synbiohub.org/public/igem/BBa_J70025/annotation1999383/range1999383/1
type: Range
persistent identity: range1999383
display id: range1999383
version: 1
direction: direction/forward
top level: BBa_J70025
start: 319
end: 330
orientation: inline

BBa_J70026
type: ComponentDefinition
persistent identity: BBa J70026
display id: BBa_J70026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cat cassette (chloramphenicol resistance)
creator: Tom Knight
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: cat gene and promoter from pC194 plasmid, Genbank V01277.
mutable notes: Mutation in sequence at base 99, ignored. GATC site eliminated at the end by truncation.
mutable provenance: Genbank V01277, by way of pKV104 from Duncan Krause. Bases 978-2006; missing T in the Krause sequence at base 99 of this sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: -10
sequence annotation: -35
sequence annotation: UP sequence
sequence annotation: cat gene
sequence annotation: RBS
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_J70026_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2002630
display id: annotation2002630
version: 1
top level: BBa_J70026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002630/range2002630/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002630/range2002630/1
type: Range
persistent identity: range2002630
display id: range2002630
version: 1
direction: direction/forward
top level: BBa_J70026
start: 215
end: 220
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002629
display id: annotation2002629
version: 1
top level: BBa_J70026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002629/range2002629/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002629/range2002629/1
type: Range
persistent identity: range2002629
display id: range2002629
version: 1
direction: direction/forward
top level: BBa_J70026
start: 192
end: 197
orientation: inline

UP sequence
type: SequenceAnnotation
persistent identity: annotation2002628
display id: annotation2002628
version: 1
top level: BBa_J70026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002628/range2002628/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002628/range2002628/1
type: Range
persistent identity: range2002628
display id: range2002628
version: 1
direction: direction/forward
top level: BBa_J70026
start: 157
end: 179
orientation: inline

cat gene
type: SequenceAnnotation
persistent identity: annotation2002632
display id: annotation2002632
version: 1
top level: BBa_J70026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002632/range2002632/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002632/range2002632/1
type: Range
persistent identity: range2002632
display id: range2002632
version: 1
direction: direction/forward
top level: BBa_J70026
start: 284
end: 934
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2002631
display id: annotation2002631
version: 1
top level: BBa_J70026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002631/range2002631/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002631/range2002631/1
type: Range
persistent identity: range2002631
display id: range2002631
version: 1
direction: direction/forward
top level: BBa_J70026
start: 271
end: 275
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2002633
display id: annotation2002633
version: 1
top level: BBa_J70026
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J70026/annotation2002633/range2002633/1

https://synbiohub.org/public/igem/BBa_J70026/annotation2002633/range2002633/1
type: Range
persistent identity: range2002633
display id: range2002633
version: 1
direction: direction/forward
top level: BBa_J70026
start: 946
end: 969
orientation: inline

BBa_J70303
type: ComponentDefinition
persistent identity: BBa J70303
display id: BBa_J70303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test Cm resistance plasmid for Mesoplasma florum
creator: Tom Knight
created: 2009-04-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.
mutable notes: Avoid assembly into a Cm resistant plasmid, as the construct is already Cm resistant.
mutable provenance: Standard assembly from registry parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70027
component: BBa_J70026
sequence annotation: BBa_J70027
sequence annotation: BBa_J70026
sequence: https://synbiohub.org/public/igem/BBa_J70303_sequence/1

BBa_J70027
type: Component
persistent identity: component2002517
display id: component2002517
version: 1
top level: BBa_J70303
access: public
definition: BBa_J70027

BBa_J70026
type: Component
persistent identity: component2002523
display id: component2002523
version: 1
top level: BBa_J70303
access: public
definition: BBa_J70026

BBa_J70027
type: SequenceAnnotation
persistent identity: annotation2002517
display id: annotation2002517
version: 1
top level: BBa_J70303
location: https://synbiohub.org/public/igem/BBa_J70303/annotation2002517/range2002517/1
component: BBa_J70027

https://synbiohub.org/public/igem/BBa_J70303/annotation2002517/range2002517/1
type: Range
persistent identity: range2002517
display id: range2002517
version: 1
direction: direction/reverse
top level: BBa_J70303
start: 1
end: 1701
orientation: inline

BBa_J70026
type: SequenceAnnotation
persistent identity: annotation2002523
display id: annotation2002523
version: 1
top level: BBa_J70303
location: https://synbiohub.org/public/igem/BBa_J70303/annotation2002523/range2002523/1
component: BBa_J70026

https://synbiohub.org/public/igem/BBa_J70303/annotation2002523/range2002523/1
type: Range
persistent identity: range2002523
display id: range2002523
version: 1
direction: direction/reverse
top level: BBa_J70303
start: 1710
end: 2739
orientation: inline

BBa_J70300
type: ComponentDefinition
persistent identity: BBa J70300
display id: BBa_J70300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong Me. florum promoter
creator: Tom Knight
created: 2009-03-28T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the highly expressed pdh e1 gene (mfl039) of Me. florum. Contains three overlapping promoters with near consensus sequence and an UP sequence.
mutable notes: None.
mutable provenance: 53556 53678 approximately from NC_006055
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: t-c mutation
sequence: https://synbiohub.org/public/igem/BBa_J70300_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2002222
display id: annotation2002222
version: 1
top level: BBa_J70300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70300/annotation2002222/range2002222/1

https://synbiohub.org/public/igem/BBa_J70300/annotation2002222/range2002222/1
type: Range
persistent identity: range2002222
display id: range2002222
version: 1
direction: direction/forward
top level: BBa_J70300
start: 77
end: 82
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002223
display id: annotation2002223
version: 1
top level: BBa_J70300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70300/annotation2002223/range2002223/1

https://synbiohub.org/public/igem/BBa_J70300/annotation2002223/range2002223/1
type: Range
persistent identity: range2002223
display id: range2002223
version: 1
direction: direction/forward
top level: BBa_J70300
start: 54
end: 60
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002224
display id: annotation2002224
version: 1
top level: BBa_J70300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70300/annotation2002224/range2002224/1

https://synbiohub.org/public/igem/BBa_J70300/annotation2002224/range2002224/1
type: Range
persistent identity: range2002224
display id: range2002224
version: 1
direction: direction/forward
top level: BBa_J70300
start: 67
end: 73
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002225
display id: annotation2002225
version: 1
top level: BBa_J70300
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70300/annotation2002225/range2002225/1

https://synbiohub.org/public/igem/BBa_J70300/annotation2002225/range2002225/1
type: Range
persistent identity: range2002225
display id: range2002225
version: 1
direction: direction/forward
top level: BBa_J70300
start: 43
end: 48
orientation: inline

t-c mutation
type: SequenceAnnotation
persistent identity: annotation2002226
display id: annotation2002226
version: 1
top level: BBa_J70300
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70300/annotation2002226/range2002226/1

https://synbiohub.org/public/igem/BBa_J70300/annotation2002226/range2002226/1
type: Range
persistent identity: range2002226
display id: range2002226
version: 1
direction: direction/forward
top level: BBa_J70300
start: 16
end: 16
orientation: inline

GmR
type: ComponentDefinition
persistent identity: BBa P1012
display id: BBa_P1012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin/kanamycin resistance cassette
creator: Tom Knight
created: 2006-03-15T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacA-aphD gene from the transposon Tn4001 conveys resistance to both Gentamycin and Kanamycin with a single two-domain resistance protein.
mutable notes: ;primers to make Biobrick version of GmR gene cassette (P1012) gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R ;P3aph-R (3' - 5') ;aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac
mutable provenance: Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: acetyl-transferase
sequence annotation: aacA-aphD (GmR, KanR)
sequence: https://synbiohub.org/public/igem/BBa_P1012_sequence/1

acetyl-transferase
type: SequenceAnnotation
persistent identity: annotation1825004
display id: annotation1825004
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825004/range1825004/1
type: Range
persistent identity: range1825004
display id: range1825004
version: 1
direction: direction/forward
top level: GmR
start: 81
end: 411
orientation: inline

aacA-aphD (GmR, KanR)
type: SequenceAnnotation
persistent identity: annotation1825005
display id: annotation1825005
version: 1
top level: GmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1

https://synbiohub.org/public/igem/BBa_P1012/annotation1825005/range1825005/1
type: Range
persistent identity: range1825005
display id: range1825005
version: 1
direction: direction/forward
top level: GmR
start: 412
end: 1851
orientation: inline

BBa_J70500
type: ComponentDefinition
persistent identity: BBa J70500
display id: BBa_J70500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pWV01 broad host range plasmid origin and rep protein
creator: Tom Knight
created: 2009-09-12T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pWV01 broad host range plasmid origin and rep protein. pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.
mutable notes: Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly.
mutable provenance: PCR of the plasmid pMG, kind gift from Prof. Jan Kok. This plasmid contains the ClaI fragment of the pWV01 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: ir-II
sequence annotation: direct repeat 1
sequence annotation: ir-I
sequence annotation: orfC (CopG)
sequence annotation: ir-IV (terminator)
sequence annotation: orfB
sequence annotation: direct repeat 3
sequence annotation: orfD
sequence annotation: direct repeat 2
sequence annotation: repA
sequence annotation: ir-III
sequence annotation: Promoter P1
sequence: https://synbiohub.org/public/igem/BBa_J70500_sequence/1

ir-II
type: SequenceAnnotation
persistent identity: annotation2020950
display id: annotation2020950
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020950/range2020950/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020950/range2020950/1
type: Range
persistent identity: range2020950
display id: range2020950
version: 1
direction: direction/forward
top level: BBa_J70500
start: 228
end: 250
orientation: inline

direct repeat 1
type: SequenceAnnotation
persistent identity: annotation2020954
display id: annotation2020954
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020954/range2020954/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020954/range2020954/1
type: Range
persistent identity: range2020954
display id: range2020954
version: 1
direction: direction/forward
top level: BBa_J70500
start: 456
end: 466
orientation: inline

ir-I
type: SequenceAnnotation
persistent identity: annotation2020949
display id: annotation2020949
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020949/range2020949/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020949/range2020949/1
type: Range
persistent identity: range2020949
display id: range2020949
version: 1
direction: direction/forward
top level: BBa_J70500
start: 50
end: 181
orientation: inline

orfC (CopG)
type: SequenceAnnotation
persistent identity: annotation2020946
display id: annotation2020946
version: 1
top level: BBa_J70500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020946/range2020946/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020946/range2020946/1
type: Range
persistent identity: range2020946
display id: range2020946
version: 1
direction: direction/forward
top level: BBa_J70500
start: 585
end: 746
orientation: inline

ir-IV (terminator)
type: SequenceAnnotation
persistent identity: annotation2020952
display id: annotation2020952
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020952/range2020952/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020952/range2020952/1
type: Range
persistent identity: range2020952
display id: range2020952
version: 1
direction: direction/forward
top level: BBa_J70500
start: 1656
end: 1697
orientation: inline

orfB
type: SequenceAnnotation
persistent identity: annotation2020945
display id: annotation2020945
version: 1
top level: BBa_J70500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020945/range2020945/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020945/range2020945/1
type: Range
persistent identity: range2020945
display id: range2020945
version: 1
direction: direction/forward
top level: BBa_J70500
start: 335
end: 544
orientation: inline

direct repeat 3
type: SequenceAnnotation
persistent identity: annotation2020956
display id: annotation2020956
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020956/range2020956/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020956/range2020956/1
type: Range
persistent identity: range2020956
display id: range2020956
version: 1
direction: direction/forward
top level: BBa_J70500
start: 479
end: 490
orientation: inline

orfD
type: SequenceAnnotation
persistent identity: annotation2020948
display id: annotation2020948
version: 1
top level: BBa_J70500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020948/range2020948/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020948/range2020948/1
type: Range
persistent identity: range2020948
display id: range2020948
version: 1
direction: direction/forward
top level: BBa_J70500
start: 1508
end: 1657
orientation: inline

direct repeat 2
type: SequenceAnnotation
persistent identity: annotation2020955
display id: annotation2020955
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020955/range2020955/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020955/range2020955/1
type: Range
persistent identity: range2020955
display id: range2020955
version: 1
direction: direction/forward
top level: BBa_J70500
start: 467
end: 478
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2020947
display id: annotation2020947
version: 1
top level: BBa_J70500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020947/range2020947/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020947/range2020947/1
type: Range
persistent identity: range2020947
display id: range2020947
version: 1
direction: direction/forward
top level: BBa_J70500
start: 813
end: 1511
orientation: inline

ir-III
type: SequenceAnnotation
persistent identity: annotation2020951
display id: annotation2020951
version: 1
top level: BBa_J70500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020951/range2020951/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020951/range2020951/1
type: Range
persistent identity: range2020951
display id: range2020951
version: 1
direction: direction/forward
top level: BBa_J70500
start: 334
end: 382
orientation: inline

Promoter P1
type: SequenceAnnotation
persistent identity: annotation2020953
display id: annotation2020953
version: 1
top level: BBa_J70500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70500/annotation2020953/range2020953/1

https://synbiohub.org/public/igem/BBa_J70500/annotation2020953/range2020953/1
type: Range
persistent identity: range2020953
display id: range2020953
version: 1
direction: direction/forward
top level: BBa_J70500
start: 522
end: 550
orientation: inline

BBa_M1000
type: ComponentDefinition
persistent identity: BBa M1000
display id: BBa_M1000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Left mosaic end for Tn5 transpososome
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: true
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Left end of a linear transpososome sequence containing the 19 bp mosaic end in the forward orientation. The sequence also contains a PvuII cut site which releases the transpososome when cut with PvuII. Constructed compound parts intended for transposition must not contain other PvuII end, or be PCR amplified with blunt end producing enzymes (Pfu, Phusion) at the PvuII cut site between CAG and CTG. This part, and the companion part M1001 provide Biobrick tools to construct Tn5 transpososomes: linear DNA sequences which can be cut in vitro from a plasmid backbone, or created by PCR, combined in vitro with purified Tn5 trasposase protein, electroportated into cells, and finally randomly inserting into the bacterial chromosome. The parts assembled between the two mosaic ends can be delivered, including antibiotic resistance markers or other functional chromosomal insertions.
mutable notes: ;biobrick left end, TA cloning overhang GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ;biobrick right end, TA cloning overhang GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ;mosaic end with cag upstream to insert PvuII cut site cagCTGTCTCTTATACACATCT ;mosaic end reverse complement with ctg upstream to insert PvuII cut site AGATGTGTATAAGAGACAGctg ;mosaic left end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG cagCTGTCTCTTATACACATCT, MEL-F ;mosaic left end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA AGATGTGTATAAGAGACAGctg, MEL-R ;mosaic right end in biobrick form construction primer F GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG AGATGTGTATAAGAGACAGctg, MER-F ;mosaic right end in biobrick form construction primer R GTT TCT TCC TGC AGC GGC CGC TAC TAG TA cagCTGTCTCTTATACACATCT, MER-R
mutable provenance: Synthesis from overlapping oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Mosaic End (forward)
sequence annotation: PvuII cut site
sequence: https://synbiohub.org/public/igem/BBa_M1000_sequence/1

Mosaic End (forward)
type: SequenceAnnotation
persistent identity: annotation1825569
display id: annotation1825569
version: 1
top level: BBa_M1000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1

https://synbiohub.org/public/igem/BBa_M1000/annotation1825569/range1825569/1
type: Range
persistent identity: range1825569
display id: range1825569
version: 1
direction: direction/forward
top level: BBa_M1000
start: 4
end: 22
orientation: inline

PvuII cut site
type: SequenceAnnotation
persistent identity: annotation1825568
display id: annotation1825568
version: 1
top level: BBa_M1000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1

https://synbiohub.org/public/igem/BBa_M1000/annotation1825568/range1825568/1
type: Range
persistent identity: range1825568
display id: range1825568
version: 1
direction: direction/forward
top level: BBa_M1000
start: 1
end: 6
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1014
display id: BBa_P1014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamicin resistance cassette aph(2)
creator: Tom Knight
created: 2006-06-10T11:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
mutable notes: XbaI site at 660 removed by mutating c->t which also improves codon usage. gatgatttttacagggatattttagagaacgaaatctac,P1014-XF gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR
mutable provenance: DQ208936
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: aph(2)
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_P1014_sequence/1

aph(2)
type: SequenceAnnotation
persistent identity: annotation2073660
display id: annotation2073660
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073660/range2073660/1
type: Range
persistent identity: range2073660
display id: range2073660
version: 1
direction: direction/forward
top level: gmR
start: 120
end: 1025
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2073661
display id: annotation2073661
version: 1
top level: gmR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1

https://synbiohub.org/public/igem/BBa_P1014/annotation2073661/range2073661/1
type: Range
persistent identity: range2073661
display id: range2073661
version: 1
direction: direction/forward
top level: gmR
start: 1040
end: 1070
orientation: inline

BBa_J70028
type: ComponentDefinition
persistent identity: BBa J70028
display id: BBa_J70028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetM tetracycline resistance cassette
creator: Tom Knight
created: 2008-11-04T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: J70025 (pBOT1 tetM promoter upstream region) + J70024 (recoded tetM gene from Geneart plasmid construction)
mutable notes: Standard assembly
mutable provenance: Assembled from J70025, J70024
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J70025
component: BBa_J70024
sequence annotation: BBa_J70025
sequence annotation: BBa_J70024
sequence: https://synbiohub.org/public/igem/BBa_J70028_sequence/1

BBa_J70025
type: Component
persistent identity: component1999340
display id: component1999340
version: 1
top level: BBa_J70028
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component1999342
display id: component1999342
version: 1
top level: BBa_J70028
access: public
definition: BBa_J70024

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation1999340
display id: annotation1999340
version: 1
top level: BBa_J70028
location: https://synbiohub.org/public/igem/BBa_J70028/annotation1999340/range1999340/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70028/annotation1999340/range1999340/1
type: Range
persistent identity: range1999340
display id: range1999340
version: 1
direction: direction/reverse
top level: BBa_J70028
start: 1
end: 345
orientation: inline

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation1999342
display id: annotation1999342
version: 1
top level: BBa_J70028
location: https://synbiohub.org/public/igem/BBa_J70028/annotation1999342/range1999342/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70028/annotation1999342/range1999342/1
type: Range
persistent identity: range1999342
display id: range1999342
version: 1
direction: direction/reverse
top level: BBa_J70028
start: 352
end: 2271
orientation: inline

BBa_J70024
type: ComponentDefinition
persistent identity: BBa J70024
display id: BBa_J70024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetM gene, recoded compromise E. coli & Mesoplasma florum
creator: Tom Knight
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum.
mutable notes: Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species.
mutable provenance: Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetM gene
sequence: https://synbiohub.org/public/igem/BBa_J70024_sequence/1

tetM gene
type: SequenceAnnotation
persistent identity: annotation1982961
display id: annotation1982961
version: 1
top level: BBa_J70024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70024/annotation1982961/range1982961/1

https://synbiohub.org/public/igem/BBa_J70024/annotation1982961/range1982961/1
type: Range
persistent identity: range1982961
display id: range1982961
version: 1
direction: direction/forward
top level: BBa_J70024
start: 1
end: 1920
orientation: inline

BBa_J70505
type: ComponentDefinition
persistent identity: BBa J70505
display id: BBa_J70505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F1 to OR-R3 GTTTCTTC T TCTAGA G aagtgctggaaaaagtatag,OR-F1 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTTAATCTCATATAACACCTC,OR-R3
mutable notes: PCR from genomic DNA
mutable provenance: Needs mutation of EcoRI sites and GATC sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70505_sequence/1

BBa_J70510
type: ComponentDefinition
persistent identity: BBa J70510
display id: BBa_J70510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F3 to OR-R4 GTTTCTTC T TCTAGA G gaatttaaaaaaatagttgagtc,OR-F3 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A cctgtaatgctgggttaac,OR-R4
mutable notes: Primer F3 mutates out an EcoRI site.
mutable provenance: PCR from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70510_sequence/1

BBa_J70508
type: ComponentDefinition
persistent identity: BBa J70508
display id: BBa_J70508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F1 to OR-R2 GTTTCTTC T TCTAGA G aagtgctggaaaaagtatag,OR-F1 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTATTAATTTTTCCTTCTTTAAC,OR-R2
mutable notes: None.
mutable provenance: PCR from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70508_sequence/1

BBa_J70509
type: ComponentDefinition
persistent identity: BBa J70509
display id: BBa_J70509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mesoplasma florum origin of replication Section of Mesoplasma florum origin of replication from OR-F3 to OR-R3 GTTTCTTC T TCTAGA G gaatttaaaaaaatagttgagtc,OR-F3 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTTAATCTCATATAACACCTC,OR-R3
mutable notes: None. Modified base in the 5' end of the binding region of the F3 primer eliminates an EcoRI site.
mutable provenance: PCR from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70509_sequence/1

BBa_J70665
type: ComponentDefinition
persistent identity: BBa J70665
display id: BBa_J70665
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MF optimized RFP reporter
creator: Tom Knight
created: 2011-04-04T11:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0011 pLac promoter with J70655 RFP
mutable notes: None
mutable provenance: composite
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_J70655
sequence annotation: BBa_R0011
sequence annotation: BBa_J70655
sequence: https://synbiohub.org/public/igem/BBa_J70665_sequence/1

BBa_R0011
type: Component
persistent identity: component2115772
display id: component2115772
version: 1
top level: BBa_J70665
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J70655
type: Component
persistent identity: component2115778
display id: component2115778
version: 1
top level: BBa_J70665
access: public
definition: BBa_J70655

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2115772
display id: annotation2115772
version: 1
top level: BBa_J70665
location: https://synbiohub.org/public/igem/BBa_J70665/annotation2115772/range2115772/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J70665/annotation2115772/range2115772/1
type: Range
persistent identity: range2115772
display id: range2115772
version: 1
direction: direction/forward
top level: BBa_J70665
start: 1
end: 54
orientation: inline

BBa_J70655
type: SequenceAnnotation
persistent identity: annotation2115778
display id: annotation2115778
version: 1
top level: BBa_J70665
location: https://synbiohub.org/public/igem/BBa_J70665/annotation2115778/range2115778/1
component: BBa_J70655

https://synbiohub.org/public/igem/BBa_J70665/annotation2115778/range2115778/1
type: Range
persistent identity: range2115778
display id: range2115778
version: 1
direction: direction/reverse
top level: BBa_J70665
start: 64
end: 762
orientation: inline

BBa_J70503
type: ComponentDefinition
persistent identity: BBa J70503
display id: BBa_J70503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mesoplasma florum origin of replication
creator: Tom Knight
created: 2009-10-02T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Section of Mesoplasma florum origin of replication from OR-F2 to OR-R3 GTTTCTTC T TCTAGA G ttttgatggttgtcaagttc,OF-F2 GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTTAATCTCATATAACACCTC,OR-R3
mutable notes: Needs mutation of EcoRI site and GATC sites.
mutable provenance: PCR from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J70503_sequence/1

BBa_J70301
type: ComponentDefinition
persistent identity: BBa J70301
display id: BBa_J70301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heat shock inducible promoter for Me. florum
creator: Tom Knight
created: 2009-03-29T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _42_41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter inhibited by the gram + hrcA gene product in normal temperature conditions. Promoter from the lon protease,
mutable notes: Low gc content, difficult PCR primers
mutable provenance: PCR from lon protease promoter of NC_006055.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: hrcA binding site
sequence: https://synbiohub.org/public/igem/BBa_J70301_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2002227
display id: annotation2002227
version: 1
top level: BBa_J70301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70301/annotation2002227/range2002227/1

https://synbiohub.org/public/igem/BBa_J70301/annotation2002227/range2002227/1
type: Range
persistent identity: range2002227
display id: range2002227
version: 1
direction: direction/forward
top level: BBa_J70301
start: 94
end: 100
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002228
display id: annotation2002228
version: 1
top level: BBa_J70301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J70301/annotation2002228/range2002228/1

https://synbiohub.org/public/igem/BBa_J70301/annotation2002228/range2002228/1
type: Range
persistent identity: range2002228
display id: range2002228
version: 1
direction: direction/forward
top level: BBa_J70301
start: 73
end: 79
orientation: inline

hrcA binding site
type: SequenceAnnotation
persistent identity: annotation2002229
display id: annotation2002229
version: 1
top level: BBa_J70301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J70301/annotation2002229/range2002229/1

https://synbiohub.org/public/igem/BBa_J70301/annotation2002229/range2002229/1
type: Range
persistent identity: range2002229
display id: range2002229
version: 1
direction: direction/forward
top level: BBa_J70301
start: 53
end: 80
orientation: inline

BBa_J70512
type: ComponentDefinition
persistent identity: BBa J70512
display id: BBa_J70512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet resistance + Mesoplasma origin fragment
creator: Tom Knight
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:08:23Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet resistance + Mesoplasma origin fragment
mutable notes: None.
mutable provenance: Assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70025
component: BBa_J70024
component: BBa_J70507
sequence annotation: BBa_J70025
sequence annotation: BBa_J70507
sequence annotation: BBa_J70024
sequence: https://synbiohub.org/public/igem/BBa_J70512_sequence/1

BBa_J70025
type: Component
persistent identity: component2028102
display id: component2028102
version: 1
top level: BBa_J70512
access: public
definition: BBa_J70025

BBa_J70024
type: Component
persistent identity: component2028104
display id: component2028104
version: 1
top level: BBa_J70512
access: public
definition: BBa_J70024

BBa_J70507
type: Component
persistent identity: component2028105
display id: component2028105
version: 1
top level: BBa_J70512
access: public
definition: BBa_J70507

BBa_J70025
type: SequenceAnnotation
persistent identity: annotation2028102
display id: annotation2028102
version: 1
top level: BBa_J70512
location: https://synbiohub.org/public/igem/BBa_J70512/annotation2028102/range2028102/1
component: BBa_J70025

https://synbiohub.org/public/igem/BBa_J70512/annotation2028102/range2028102/1
type: Range
persistent identity: range2028102
display id: range2028102
version: 1
direction: direction/reverse
top level: BBa_J70512
start: 1
end: 345
orientation: inline

BBa_J70507
type: SequenceAnnotation
persistent identity: annotation2028105
display id: annotation2028105
version: 1
top level: BBa_J70512
location: https://synbiohub.org/public/igem/BBa_J70512/annotation2028105/range2028105/1
component: BBa_J70507

https://synbiohub.org/public/igem/BBa_J70512/annotation2028105/range2028105/1
type: Range
persistent identity: range2028105
display id: range2028105
version: 1
direction: direction/reverse
top level: BBa_J70512
start: 2280
end: 2667
orientation: inline

BBa_J70024
type: SequenceAnnotation
persistent identity: annotation2028104
display id: annotation2028104
version: 1
top level: BBa_J70512
location: https://synbiohub.org/public/igem/BBa_J70512/annotation2028104/range2028104/1
component: BBa_J70024

https://synbiohub.org/public/igem/BBa_J70512/annotation2028104/range2028104/1
type: Range
persistent identity: range2028104
display id: range2028104
version: 1
direction: direction/reverse
top level: BBa_J70512
start: 352
end: 2271
orientation: inline

gmR
type: ComponentDefinition
persistent identity: BBa P1013
display id: BBa_P1013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gentamycin resistance cassette (aacC1 gene)
creator: Tom Knight
created: 2006-03-16T12:00:00Z
modified: 2015-05-08T01:14:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The aacC1 gene conveys resistance to gentamycin only. It is derived from a Tn21 series transposon (Wohlleben89) found on the R1033 plasmid (Pseudomonas aeruginosa). This part is created by PCR from the pJAT8 plasmid (Keastling lab) which in turn is derived from pJN105 (Newman and Fuqua).
mutable notes: pJAT8 plasmid edited with primers: GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ccttgtcgccttgcgtataa,aacC1-F GTT TCT TCC TGC AGC GGC CGC TAC TAG TA atctcggcttgaacgaattg,aacC1-R
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35 / -10 promoter sites
sequence annotation: RBS
sequence annotation: aacC1 coding sequence
sequence: https://synbiohub.org/public/igem/BBa_P1013_sequence/1

-35 / -10 promoter sites
type: SequenceAnnotation
persistent identity: annotation1826613
display id: annotation1826613
version: 1
top level: gmR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826613/range1826613/1
type: Range
persistent identity: range1826613
display id: range1826613
version: 1
direction: direction/forward
top level: gmR
start: 71
end: 99
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1826615
display id: annotation1826615
version: 1
top level: gmR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826615/range1826615/1
type: Range
persistent identity: range1826615
display id: range1826615
version: 1
direction: direction/forward
top level: gmR
start: 276
end: 279
orientation: inline

aacC1 coding sequence
type: SequenceAnnotation
persistent identity: annotation1826614
display id: annotation1826614
version: 1
top level: gmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1

https://synbiohub.org/public/igem/BBa_P1013/annotation1826614/range1826614/1
type: Range
persistent identity: range1826614
display id: range1826614
version: 1
direction: direction/forward
top level: gmR
start: 288
end: 821
orientation: inline

BBa_J70005
type: ComponentDefinition
persistent identity: BBa J70005
display id: BBa_J70005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Click Beetle Luciferase Red
creator: Spenser Skates
created: 2008-03-28T12:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 2541
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Click beetle luciferase derived from Promega's red/green system, modified using the E. coli/Mesoplasma florum compromise codon table on open wet ware. Removal of all restriction sites necessary to make fully Biobrick compliant.
mutable notes: -
mutable provenance: Derrived from Promega's click beetle luciferase red/green system, originally designed to function in mammalian cells. From the open reading frame of the pCBR vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J70005_sequence/1