//chassis/organelle
type: Collection
persistent identity: chassis organelle
display id: chassis_organelle
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //chassis/organelle/mitochondrion
member: //chassis/organelle/chloroplast

//chassis/organelle/mitochondrion
type: Collection
persistent identity: chassis organelle mitochondrion
display id: chassis_organelle_mitochondrion
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: COX1 promo
member: BBa_K809106
member: ROP+P
member: mt ori7
member: BBa_K809101
member: COX3 termi
member: OLI1 RBS
member: BBa_K809108
member: BBa_K809504
member: P+ROP
member: SP_REX
member: UPcox1
member: mtGFP
member: COB termin
member: AI1 RBS
member: BBa_K809107
member: BBa_K809503

//chassis/organelle/chloroplast
type: Collection
persistent identity: chassis organelle chloroplast
display id: chassis_organelle_chloroplast
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1479016

BBa_K1479016
type: ComponentDefinition
persistent identity: BBa K1479016
display id: BBa_K1479016
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: adsfsdfasdfasdf
owned by: james
owned by: myers
mutable description: fsadfasdfasdfasfdasdfa
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable notes: sadfasdfadfsdfa
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 1
sequence annotation: 4

1
type: SequenceAnnotation
persistent identity: annotation2418316
display id: annotation2418316
version: 1
top level: BBa_K1479016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1
type: Range
persistent identity: range2418316
display id: range2418316
version: 1
top level: BBa_K1479016
direction: direction/forward
start: 2
end: 3
orientation: inline

4
type: SequenceAnnotation
persistent identity: annotation2418317
display id: annotation2418317
version: 1
top level: BBa_K1479016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1
type: Range
persistent identity: range2418317
display id: range2418317
version: 1
top level: BBa_K1479016
direction: direction/forward
start: 5
end: 6
orientation: inline

ROP+P
type: ComponentDefinition
persistent identity: BBa K809403
display id: BBa_K809403
version: 1
was generated by: iGEM to SBOL conversion
description: ROP+Promoter in mitochondria
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: 1. Brekasis, D. and M. S. Paget (2003). "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)." EMBO J 22(18): 4856-4865. 2. Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta. 3. http://regulondb.ccg.unam.mx/
owned by: james
owned by: myers
mutable description: This is regulatory sequence which can be repressed by binding of REX on ROP site.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: With different place of ROP and promoter, the effect of REX binding on ROP might differs.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K809403_sequence/1

BBa_K809504
type: ComponentDefinition
persistent identity: BBa K809504
display id: BBa_K809504
version: 1
was generated by: iGEM to SBOL conversion
description: ROP+P+OLI1 RBS+mtGFP+COX3 terminator
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809403 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005
owned by: james
owned by: myers
mutable description: This biobrick is to produce GFP with the control of REX binding on ROP site.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: All parts should be correctly linked without frame shift and Restriction Enzyme cutting site.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809005
component: BBa_K809001
component: BBa_K809403
component: BBa_K809009
sequence annotation: BBa_K809001
sequence annotation: BBa_K809403
sequence annotation: BBa_K809005
sequence annotation: BBa_K809009
sequence: https://synbiohub.org/public/igem/BBa_K809504_sequence/1

BBa_K809005
type: Component
persistent identity: component2196294
display id: component2196294
version: 1
top level: BBa_K809504
access: public
definition: COX3 termi

BBa_K809001
type: Component
persistent identity: component2196292
display id: component2196292
version: 1
top level: BBa_K809504
access: public
definition: mtGFP

BBa_K809403
type: Component
persistent identity: component2196289
display id: component2196289
version: 1
top level: BBa_K809504
access: public
definition: ROP+P

BBa_K809009
type: Component
persistent identity: component2196290
display id: component2196290
version: 1
top level: BBa_K809504
access: public
definition: OLI1 RBS

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2196292
display id: annotation2196292
version: 1
top level: BBa_K809504
location: https://synbiohub.org/public/igem/BBa_K809504/annotation2196292/range2196292/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809504/annotation2196292/range2196292/1
type: Range
persistent identity: range2196292
display id: range2196292
version: 1
top level: BBa_K809504
direction: direction/reverse
start: 170
end: 886
orientation: inline

BBa_K809403
type: SequenceAnnotation
persistent identity: annotation2196289
display id: annotation2196289
version: 1
top level: BBa_K809504
location: https://synbiohub.org/public/igem/BBa_K809504/annotation2196289/range2196289/1
component: BBa_K809403

https://synbiohub.org/public/igem/BBa_K809504/annotation2196289/range2196289/1
type: Range
persistent identity: range2196289
display id: range2196289
version: 1
top level: BBa_K809504
direction: direction/reverse
start: 1
end: 43
orientation: inline

BBa_K809005
type: SequenceAnnotation
persistent identity: annotation2196294
display id: annotation2196294
version: 1
top level: BBa_K809504
location: https://synbiohub.org/public/igem/BBa_K809504/annotation2196294/range2196294/1
component: BBa_K809005

https://synbiohub.org/public/igem/BBa_K809504/annotation2196294/range2196294/1
type: Range
persistent identity: range2196294
display id: range2196294
version: 1
top level: BBa_K809504
direction: direction/reverse
start: 895
end: 943
orientation: inline

BBa_K809009
type: SequenceAnnotation
persistent identity: annotation2196290
display id: annotation2196290
version: 1
top level: BBa_K809504
location: https://synbiohub.org/public/igem/BBa_K809504/annotation2196290/range2196290/1
component: BBa_K809009

https://synbiohub.org/public/igem/BBa_K809504/annotation2196290/range2196290/1
type: Range
persistent identity: range2196290
display id: range2196290
version: 1
top level: BBa_K809504
direction: direction/reverse
start: 50
end: 163
orientation: inline

UPcox1
type: ComponentDefinition
persistent identity: BBa K809003
display id: BBa_K809003
version: 1
was generated by: iGEM to SBOL conversion
description: Up elements of cox1 gene
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: Synthesized from LifeTechonology
owned by: james
owned by: myers
mutable description: Up elements (including promoter and RBS) of cox2 gene from Saccharomyces cerevisiae mitochondrial DNA.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Designed by Qiwu XU, BioBrick restriction enzyme sites added.
star: false
creator: Qiwu XU
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cox1 up elements
sequence: https://synbiohub.org/public/igem/BBa_K809003_sequence/1

cox1 up elements
type: SequenceAnnotation
persistent identity: annotation2188525
display id: annotation2188525
version: 1
top level: UPcox1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K809003/annotation2188525/range2188525/1

https://synbiohub.org/public/igem/BBa_K809003/annotation2188525/range2188525/1
type: Range
persistent identity: range2188525
display id: range2188525
version: 1
top level: UPcox1
direction: direction/forward
start: 1
end: 1
orientation: inline

cox1 up elements
type: SequenceAnnotation
persistent identity: annotation2188526
display id: annotation2188526
version: 1
top level: UPcox1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K809003/annotation2188526/range2188526/1

https://synbiohub.org/public/igem/BBa_K809003/annotation2188526/range2188526/1
type: Range
persistent identity: range2188526
display id: range2188526
version: 1
top level: UPcox1
direction: direction/forward
start: 497
end: 497
orientation: inline

mtGFP
type: ComponentDefinition
persistent identity: BBa K809001
display id: BBa_K809001
version: 1
was generated by: iGEM to SBOL conversion
description: Mitochondrial GFP
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: Mitochondrial GFP
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: ..
star: false
creator: Qiwu XU
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mt gfp
sequence: https://synbiohub.org/public/igem/BBa_K809001_sequence/1

mt gfp
type: SequenceAnnotation
persistent identity: annotation2188517
display id: annotation2188517
version: 1
top level: mtGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K809001/annotation2188517/range2188517/1

https://synbiohub.org/public/igem/BBa_K809001/annotation2188517/range2188517/1
type: Range
persistent identity: range2188517
display id: range2188517
version: 1
top level: mtGFP
direction: direction/forward
start: 1
end: 714
orientation: inline

mt ori7
type: ComponentDefinition
persistent identity: BBa K809002
display id: BBa_K809002
version: 1
was generated by: iGEM to SBOL conversion
description: ori7 region of mtDNA
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: NCBI database of Saccharomyces cerevisiae's genome
owned by: james
owned by: myers
mutable description: ori 7 region of Saccharomyces cerevisiae mtDNA, works as replication initial site.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Qiwu XU
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: ori7
sequence: https://synbiohub.org/public/igem/BBa_K809002_sequence/1

ori7
type: SequenceAnnotation
persistent identity: annotation2188522
display id: annotation2188522
version: 1
top level: mt ori7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188522/range2188522/1
type: Range
persistent identity: range2188522
display id: range2188522
version: 1
top level: mt ori7
direction: direction/forward
start: 1
end: 1
orientation: inline

ori7
type: SequenceAnnotation
persistent identity: annotation2188523
display id: annotation2188523
version: 1
top level: mt ori7
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1

https://synbiohub.org/public/igem/BBa_K809002/annotation2188523/range2188523/1
type: Range
persistent identity: range2188523
display id: range2188523
version: 1
top level: mt ori7
direction: direction/forward
start: 378
end: 378
orientation: inline

BBa_K809503
type: ComponentDefinition
persistent identity: BBa K809503
display id: BBa_K809503
version: 1
was generated by: iGEM to SBOL conversion
description: P+ROP+OLI1 RBS+mtGFP+COX3 terminator
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809402 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005
owned by: james
owned by: myers
mutable description: This biobrick is to produce GFP with the control of REX binding on ROP site.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: All parts should be correctly linked without frame shift and Restriction Enzyme cutting site.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809402
component: BBa_K809009
component: BBa_K809001
component: BBa_K809005
sequence annotation: BBa_K809001
sequence annotation: BBa_K809005
sequence annotation: BBa_K809009
sequence annotation: BBa_K809402
sequence: https://synbiohub.org/public/igem/BBa_K809503_sequence/1

BBa_K809402
type: Component
persistent identity: component2196295
display id: component2196295
version: 1
top level: BBa_K809503
access: public
definition: P+ROP

BBa_K809009
type: Component
persistent identity: component2196296
display id: component2196296
version: 1
top level: BBa_K809503
access: public
definition: OLI1 RBS

BBa_K809001
type: Component
persistent identity: component2196298
display id: component2196298
version: 1
top level: BBa_K809503
access: public
definition: mtGFP

BBa_K809005
type: Component
persistent identity: component2196300
display id: component2196300
version: 1
top level: BBa_K809503
access: public
definition: COX3 termi

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2196298
display id: annotation2196298
version: 1
top level: BBa_K809503
location: https://synbiohub.org/public/igem/BBa_K809503/annotation2196298/range2196298/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809503/annotation2196298/range2196298/1
type: Range
persistent identity: range2196298
display id: range2196298
version: 1
top level: BBa_K809503
direction: direction/reverse
start: 170
end: 886
orientation: inline

BBa_K809005
type: SequenceAnnotation
persistent identity: annotation2196300
display id: annotation2196300
version: 1
top level: BBa_K809503
location: https://synbiohub.org/public/igem/BBa_K809503/annotation2196300/range2196300/1
component: BBa_K809005

https://synbiohub.org/public/igem/BBa_K809503/annotation2196300/range2196300/1
type: Range
persistent identity: range2196300
display id: range2196300
version: 1
top level: BBa_K809503
direction: direction/reverse
start: 895
end: 943
orientation: inline

BBa_K809009
type: SequenceAnnotation
persistent identity: annotation2196296
display id: annotation2196296
version: 1
top level: BBa_K809503
location: https://synbiohub.org/public/igem/BBa_K809503/annotation2196296/range2196296/1
component: BBa_K809009

https://synbiohub.org/public/igem/BBa_K809503/annotation2196296/range2196296/1
type: Range
persistent identity: range2196296
display id: range2196296
version: 1
top level: BBa_K809503
direction: direction/reverse
start: 50
end: 163
orientation: inline

BBa_K809402
type: SequenceAnnotation
persistent identity: annotation2196295
display id: annotation2196295
version: 1
top level: BBa_K809503
location: https://synbiohub.org/public/igem/BBa_K809503/annotation2196295/range2196295/1
component: BBa_K809402

https://synbiohub.org/public/igem/BBa_K809503/annotation2196295/range2196295/1
type: Range
persistent identity: range2196295
display id: range2196295
version: 1
top level: BBa_K809503
direction: direction/reverse
start: 1
end: 43
orientation: inline

REX
type: ComponentDefinition
persistent identity: BBa K809401
display id: BBa_K809401
version: 1
was generated by: iGEM to SBOL conversion
description: REX coding sequence
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: SU9 comes from yeast gemome. REX was modified to suitable for expression in yeast.
owned by: james
owned by: myers
mutable description: This biobrick is the coding sequence of our SU9+REX in yeast cytoplasm. It is used to construct our final biobricks which express REX in the cytoplasm and then transport REX into mitochondrial matrix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The signal peptid must be able to work efficiently, and can transport REX into mitochondrial matrix.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REX
sequence: https://synbiohub.org/public/igem/BBa_K809401_sequence/1

REX
type: SequenceAnnotation
persistent identity: annotation2188980
display id: annotation2188980
version: 1
top level: REX
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K809401/annotation2188980/range2188980/1

https://synbiohub.org/public/igem/BBa_K809401/annotation2188980/range2188980/1
type: Range
persistent identity: range2188980
display id: range2188980
version: 1
top level: REX
direction: direction/forward
start: 1
end: 774
orientation: inline

BBa_K809107
type: ComponentDefinition
persistent identity: BBa K809107
display id: BBa_K809107
version: 1
was generated by: iGEM to SBOL conversion
description: Efficiency test of COB Terminator
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809004 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
owned by: james
owned by: myers
mutable description: This biobrick is used to test the efficient of COB terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The biobrick should be usable and all the biobricks won't interfere with each other.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809007
component: BBa_K809004
component: BBa_K809009
component: BBa_K809001
sequence annotation: BBa_K809009
sequence annotation: BBa_K809004
sequence annotation: BBa_K809001
sequence annotation: BBa_K809007
sequence: https://synbiohub.org/public/igem/BBa_K809107_sequence/1

BBa_K809007
type: Component
persistent identity: component2199459
display id: component2199459
version: 1
top level: BBa_K809107
access: public
definition: COX1 promo

BBa_K809004
type: Component
persistent identity: component2199461
display id: component2199461
version: 1
top level: BBa_K809107
access: public
definition: COB termin

BBa_K809009
type: Component
persistent identity: component2199462
display id: component2199462
version: 1
top level: BBa_K809107
access: public
definition: OLI1 RBS

BBa_K809001
type: Component
persistent identity: component2199464
display id: component2199464
version: 1
top level: BBa_K809107
access: public
definition: mtGFP

BBa_K809009
type: SequenceAnnotation
persistent identity: annotation2199462
display id: annotation2199462
version: 1
top level: BBa_K809107
location: https://synbiohub.org/public/igem/BBa_K809107/annotation2199462/range2199462/1
component: BBa_K809009

https://synbiohub.org/public/igem/BBa_K809107/annotation2199462/range2199462/1
type: Range
persistent identity: range2199462
display id: range2199462
version: 1
top level: BBa_K809107
direction: direction/reverse
start: 76
end: 189
orientation: inline

BBa_K809004
type: SequenceAnnotation
persistent identity: annotation2199461
display id: annotation2199461
version: 1
top level: BBa_K809107
location: https://synbiohub.org/public/igem/BBa_K809107/annotation2199461/range2199461/1
component: BBa_K809004

https://synbiohub.org/public/igem/BBa_K809107/annotation2199461/range2199461/1
type: Range
persistent identity: range2199461
display id: range2199461
version: 1
top level: BBa_K809107
direction: direction/reverse
start: 20
end: 69
orientation: inline

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2199464
display id: annotation2199464
version: 1
top level: BBa_K809107
location: https://synbiohub.org/public/igem/BBa_K809107/annotation2199464/range2199464/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809107/annotation2199464/range2199464/1
type: Range
persistent identity: range2199464
display id: range2199464
version: 1
top level: BBa_K809107
direction: direction/reverse
start: 196
end: 912
orientation: inline

BBa_K809007
type: SequenceAnnotation
persistent identity: annotation2199459
display id: annotation2199459
version: 1
top level: BBa_K809107
location: https://synbiohub.org/public/igem/BBa_K809107/annotation2199459/range2199459/1
component: BBa_K809007

https://synbiohub.org/public/igem/BBa_K809107/annotation2199459/range2199459/1
type: Range
persistent identity: range2199459
display id: range2199459
version: 1
top level: BBa_K809107
direction: direction/reverse
start: 1
end: 11
orientation: inline

BBa_K809101
type: ComponentDefinition
persistent identity: BBa K809101
display id: BBa_K809101
version: 1
was generated by: iGEM to SBOL conversion
description: Mitochondrial shuttle plasmid carried mtGFP
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: Mitochondrial expression vector, with initial site ori7, up elements of cox2 gene (including promoter and RBS), and mtGFP.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Senwei TANG, Qiwu XU
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K809002
component: BBa_K809003
component: BBa_K809001
sequence annotation: BBa_K809001
sequence annotation: BBa_K809002
sequence annotation: BBa_K809003
sequence: https://synbiohub.org/public/igem/BBa_K809101_sequence/1

BBa_K809002
type: Component
persistent identity: component2192205
display id: component2192205
version: 1
top level: BBa_K809101
access: public
definition: mt ori7

BBa_K809003
type: Component
persistent identity: component2192206
display id: component2192206
version: 1
top level: BBa_K809101
access: public
definition: UPcox1

BBa_K809001
type: Component
persistent identity: component2192208
display id: component2192208
version: 1
top level: BBa_K809101
access: public
definition: mtGFP

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2192208
display id: annotation2192208
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192208/range2192208/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809101/annotation2192208/range2192208/1
type: Range
persistent identity: range2192208
display id: range2192208
version: 1
top level: BBa_K809101
direction: direction/reverse
start: 890
end: 1606
orientation: inline

BBa_K809002
type: SequenceAnnotation
persistent identity: annotation2192205
display id: annotation2192205
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192205/range2192205/1
component: BBa_K809002

https://synbiohub.org/public/igem/BBa_K809101/annotation2192205/range2192205/1
type: Range
persistent identity: range2192205
display id: range2192205
version: 1
top level: BBa_K809101
direction: direction/reverse
start: 1
end: 378
orientation: inline

BBa_K809003
type: SequenceAnnotation
persistent identity: annotation2192206
display id: annotation2192206
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192206/range2192206/1
component: BBa_K809003

https://synbiohub.org/public/igem/BBa_K809101/annotation2192206/range2192206/1
type: Range
persistent identity: range2192206
display id: range2192206
version: 1
top level: BBa_K809101
direction: direction/reverse
start: 387
end: 883
orientation: inline

COX3 termi
type: ComponentDefinition
persistent identity: BBa K809005
display id: BBa_K809005
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator of Yeast mitochondrial gene COX3
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
owned by: james
owned by: myers
mutable description: This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809005_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194193
display id: annotation2194193
version: 1
top level: COX3 termi
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1

https://synbiohub.org/public/igem/BBa_K809005/annotation2194193/range2194193/1
type: Range
persistent identity: range2194193
display id: range2194193
version: 1
top level: COX3 termi
direction: direction/forward
start: 16
end: 34
orientation: inline

BBa_K809108
type: ComponentDefinition
persistent identity: BBa K809108
display id: BBa_K809108
version: 1
was generated by: iGEM to SBOL conversion
description: Efficiency test of Q0255 Terminator
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809006 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
owned by: james
owned by: myers
mutable description: This biobrick is used to test the efficient of Q0255 terminator.
discontinued: false
dominant: true
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The biobrick should be usable and all the biobricks won't interfere with each other.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809005
component: BBa_K809009
component: BBa_K809007
component: BBa_K809001
sequence annotation: BBa_K809007
sequence annotation: BBa_K809009
sequence annotation: BBa_K809001
sequence annotation: BBa_K809005
sequence: https://synbiohub.org/public/igem/BBa_K809108_sequence/1

BBa_K809005
type: Component
persistent identity: component2199467
display id: component2199467
version: 1
top level: BBa_K809108
access: public
definition: COX3 termi

BBa_K809009
type: Component
persistent identity: component2199468
display id: component2199468
version: 1
top level: BBa_K809108
access: public
definition: OLI1 RBS

BBa_K809007
type: Component
persistent identity: component2199465
display id: component2199465
version: 1
top level: BBa_K809108
access: public
definition: COX1 promo

BBa_K809001
type: Component
persistent identity: component2199470
display id: component2199470
version: 1
top level: BBa_K809108
access: public
definition: mtGFP

BBa_K809007
type: SequenceAnnotation
persistent identity: annotation2199465
display id: annotation2199465
version: 1
top level: BBa_K809108
location: https://synbiohub.org/public/igem/BBa_K809108/annotation2199465/range2199465/1
component: BBa_K809007

https://synbiohub.org/public/igem/BBa_K809108/annotation2199465/range2199465/1
type: Range
persistent identity: range2199465
display id: range2199465
version: 1
top level: BBa_K809108
direction: direction/reverse
start: 1
end: 11
orientation: inline

BBa_K809009
type: SequenceAnnotation
persistent identity: annotation2199468
display id: annotation2199468
version: 1
top level: BBa_K809108
location: https://synbiohub.org/public/igem/BBa_K809108/annotation2199468/range2199468/1
component: BBa_K809009

https://synbiohub.org/public/igem/BBa_K809108/annotation2199468/range2199468/1
type: Range
persistent identity: range2199468
display id: range2199468
version: 1
top level: BBa_K809108
direction: direction/reverse
start: 75
end: 188
orientation: inline

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2199470
display id: annotation2199470
version: 1
top level: BBa_K809108
location: https://synbiohub.org/public/igem/BBa_K809108/annotation2199470/range2199470/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809108/annotation2199470/range2199470/1
type: Range
persistent identity: range2199470
display id: range2199470
version: 1
top level: BBa_K809108
direction: direction/reverse
start: 195
end: 911
orientation: inline

BBa_K809005
type: SequenceAnnotation
persistent identity: annotation2199467
display id: annotation2199467
version: 1
top level: BBa_K809108
location: https://synbiohub.org/public/igem/BBa_K809108/annotation2199467/range2199467/1
component: BBa_K809005

https://synbiohub.org/public/igem/BBa_K809108/annotation2199467/range2199467/1
type: Range
persistent identity: range2199467
display id: range2199467
version: 1
top level: BBa_K809108
direction: direction/reverse
start: 20
end: 68
orientation: inline

OLI1 RBS
type: ComponentDefinition
persistent identity: BBa K809009
display id: BBa_K809009
version: 1
was generated by: iGEM to SBOL conversion
description: RBS sequence of yeast mitochondrial gene OLI1
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
owned by: james
owned by: myers
mutable description: This is the RBS region for OLI1 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence between the last ATG codon and CDS on the 3'UTR DNA sequence is believed to contain RBS. With some modifications, we made those sequences BioBricks.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K809009_sequence/1

P+ROP
type: ComponentDefinition
persistent identity: BBa K809402
display id: BBa_K809402
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter+ROP in mitochondria
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: 1. Brekasis, D. and M. S. Paget (2003). "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)." EMBO J 22(18): 4856-4865. 2. Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta.
owned by: james
owned by: myers
mutable description: This is regulatory sequence which can be repressed by binding of REX on ROP site.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: With different place of ROP and promoter, the effect of REX binding on ROP might differs.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K809402_sequence/1

COX1 promo
type: ComponentDefinition
persistent identity: BBa K809007
display id: BBa_K809007
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter of Yeast mitochondrial gene COX1/Oli1/15s rRNA
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta.
owned by: james
owned by: myers
mutable description: This is the promoter region for COX1/Oli1/15s rRNA gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence is synthesized based on the sequences retrived.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K809007_sequence/1

AI1 RBS
type: ComponentDefinition
persistent identity: BBa K809008
display id: BBa_K809008
version: 1
was generated by: iGEM to SBOL conversion
description: RBS sequence of yeast mitochondrial gene AI1
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
owned by: james
owned by: myers
mutable description: This is the RBS region for AI1 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence between the last ATG codon and CDS on the 3'UTR DNA sequence is believed to contain RBS. With some modifications, we made those sequences BioBricks.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K809008_sequence/1

SP_REX
type: ComponentDefinition
persistent identity: BBa K809501
display id: BBa_K809501
version: 1
was generated by: iGEM to SBOL conversion
description: REX+SPzim17
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:27Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809201 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809401
owned by: james
owned by: myers
mutable description: REX is tagged with signal peptide of ZIM17, thus can be targetted into mitochondrial matrix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part BBa_K809201 and BBa_K809401 should be correctly linked without frame shift
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809401
component: BBa_K809201
sequence annotation: BBa_K809201
sequence annotation: BBa_K809401
sequence: https://synbiohub.org/public/igem/BBa_K809501_sequence/1

BBa_K809401
type: Component
persistent identity: component2195678
display id: component2195678
version: 1
top level: SP_REX
access: public
definition: REX

BBa_K809201
type: Component
persistent identity: component2195676
display id: component2195676
version: 1
top level: SP_REX
access: public
definition: SPzim17

BBa_K809201
type: SequenceAnnotation
persistent identity: annotation2195676
display id: annotation2195676
version: 1
top level: SP_REX
location: https://synbiohub.org/public/igem/BBa_K809501/annotation2195676/range2195676/1
component: BBa_K809201

https://synbiohub.org/public/igem/BBa_K809501/annotation2195676/range2195676/1
type: Range
persistent identity: range2195676
display id: range2195676
version: 1
top level: SP_REX
direction: direction/reverse
start: 1
end: 141
orientation: inline

BBa_K809401
type: SequenceAnnotation
persistent identity: annotation2195678
display id: annotation2195678
version: 1
top level: SP_REX
location: https://synbiohub.org/public/igem/BBa_K809501/annotation2195678/range2195678/1
component: BBa_K809401

https://synbiohub.org/public/igem/BBa_K809501/annotation2195678/range2195678/1
type: Range
persistent identity: range2195678
display id: range2195678
version: 1
top level: SP_REX
direction: direction/reverse
start: 148
end: 924
orientation: inline

SPzim17
type: ComponentDefinition
persistent identity: BBa K809201
display id: BBa_K809201
version: 1
was generated by: iGEM to SBOL conversion
description: signal peptide of ZIM17
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: Zim17, a novel zinc finger protein essential for protein import into mitochondria. J Biol Chem. 2004 Nov 26 ;279(48):50243-9. Epub 2004 Sep 21 . http://www.uniprot.org/uniprot/P42844
owned by: james
owned by: myers
mutable description: this part is the sequence of transit signal peptide of ZIM17. It can help protein target to the mitochondrial matrix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12995
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: it is synthesized based on the complete sequence of signal peptide of ZIM17
star: false
creator: Luohao Xu
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K809201_sequence/1

COB termin
type: ComponentDefinition
persistent identity: BBa K809004
display id: BBa_K809004
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator of Yeast mitochondrial gene COB
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: It comes from Saccharomyces cerevisiae S288C mito-chromosome region
owned by: james
owned by: myers
mutable description: This is the terminator region for COB gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We modelled the RNA secondary structure of COB 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_K809004_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation2194600
display id: annotation2194600
version: 1
top level: COB termin
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K809004/annotation2194600/range2194600/1

https://synbiohub.org/public/igem/BBa_K809004/annotation2194600/range2194600/1
type: Range
persistent identity: range2194600
display id: range2194600
version: 1
top level: COB termin
direction: direction/forward
start: 11
end: 40
orientation: inline

BBa_K809106
type: ComponentDefinition
persistent identity: BBa K809106
display id: BBa_K809106
version: 1
was generated by: iGEM to SBOL conversion
description: Efficiency test of COX3 Terminator
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: http://partsregistry.org/wiki/index.php?title=Part:BBa_K809007 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809009 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
owned by: james
owned by: myers
mutable description: This biobrick is used to test the efficient of COX3 terminator.
discontinued: false
dominant: true
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The biobrick should be usable and all the biobricks won't interfere with each other.
star: false
creator: Xiaopeng Xu
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K809001
component: BBa_K809009
component: BBa_K809007
component: BBa_K809005
sequence annotation: BBa_K809009
sequence annotation: BBa_K809005
sequence annotation: BBa_K809007
sequence annotation: BBa_K809001
sequence: https://synbiohub.org/public/igem/BBa_K809106_sequence/1

BBa_K809001
type: Component
persistent identity: component2199458
display id: component2199458
version: 1
top level: BBa_K809106
access: public
definition: mtGFP

BBa_K809009
type: Component
persistent identity: component2199456
display id: component2199456
version: 1
top level: BBa_K809106
access: public
definition: OLI1 RBS

BBa_K809007
type: Component
persistent identity: component2199453
display id: component2199453
version: 1
top level: BBa_K809106
access: public
definition: COX1 promo

BBa_K809005
type: Component
persistent identity: component2199455
display id: component2199455
version: 1
top level: BBa_K809106
access: public
definition: COX3 termi

BBa_K809009
type: SequenceAnnotation
persistent identity: annotation2199456
display id: annotation2199456
version: 1
top level: BBa_K809106
location: https://synbiohub.org/public/igem/BBa_K809106/annotation2199456/range2199456/1
component: BBa_K809009

https://synbiohub.org/public/igem/BBa_K809106/annotation2199456/range2199456/1
type: Range
persistent identity: range2199456
display id: range2199456
version: 1
top level: BBa_K809106
direction: direction/reverse
start: 75
end: 188
orientation: inline

BBa_K809005
type: SequenceAnnotation
persistent identity: annotation2199455
display id: annotation2199455
version: 1
top level: BBa_K809106
location: https://synbiohub.org/public/igem/BBa_K809106/annotation2199455/range2199455/1
component: BBa_K809005

https://synbiohub.org/public/igem/BBa_K809106/annotation2199455/range2199455/1
type: Range
persistent identity: range2199455
display id: range2199455
version: 1
top level: BBa_K809106
direction: direction/reverse
start: 20
end: 68
orientation: inline

BBa_K809007
type: SequenceAnnotation
persistent identity: annotation2199453
display id: annotation2199453
version: 1
top level: BBa_K809106
location: https://synbiohub.org/public/igem/BBa_K809106/annotation2199453/range2199453/1
component: BBa_K809007

https://synbiohub.org/public/igem/BBa_K809106/annotation2199453/range2199453/1
type: Range
persistent identity: range2199453
display id: range2199453
version: 1
top level: BBa_K809106
direction: direction/reverse
start: 1
end: 11
orientation: inline

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2199458
display id: annotation2199458
version: 1
top level: BBa_K809106
location: https://synbiohub.org/public/igem/BBa_K809106/annotation2199458/range2199458/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809106/annotation2199458/range2199458/1
type: Range
persistent identity: range2199458
display id: range2199458
version: 1
top level: BBa_K809106
direction: direction/reverse
start: 195
end: 911
orientation: inline

https://synbiohub.org/public/igem/BBa_K809008_sequence/1
type: Sequence
persistent identity: BBa K809008 sequence
display id: BBa_K809008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatataataataataatattattaaaattaatatataaaaaaaaagtaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809003_sequence/1
type: Sequence
persistent identity: BBa K809003 sequence
display id: BBa_K809003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatatataatattaaataataagtattgatataagtaatagatataataataatattattaatattttatataaataatattaataatatagattatgaaagagagtattaatatcattaaatatatatatatgttatataatttaaatgattttaatatatatatatatattatattatagattatgatacatttatataaataatatatatataaaaattaattatactattactttataatataataatatttatttataaagatataaaagaattgtttaaagttataactaaaatattatatagtattcattaataattaatattataaattcaactattgttatatttataaatagaataatatattattatcctttaagatataacaataattatttaaattaaattaaattaaatttaattaatttttttttttaatgaatataataataataatattattaaaattaatatataaaaaaaaagtaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809504_sequence/1
type: Sequence
persistent identity: BBa K809504 sequence
display id: BBa_K809504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgcttgtgaatgtgaacgcgttcacaagcgtatataagtaattactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataatactagagaaaactcctaacggggttcccgcgaagcgggaactaataataatataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809101_sequence/1
type: Sequence
persistent identity: BBa K809101 sequence
display id: BBa_K809101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaatagggggagggggtgggtgataataaccagaatattcaataaatacagagcacacattacataaattttataatataaccaatataaaataaaattaaaataattaatatatatatataaatataataaattattatatataaatatatataatttttataataaatattataatattatataaataaataattataatatataataaatatataataataataaaaatattaacaatataataaaaatttataatataaatataaattataaataagttaaattaataaaataataaatgttaacaagaagatatctggggtcccattaataattattattttcaataataattgggaccccccaccattatatactagagaatatataatattaaataataagtattgatataagtaatagatataataataatattattaatattttatataaataatattaataatatagattatgaaagagagtattaatatcattaaatatatatatatgttatataatttaaatgattttaatatatatatatatattatattatagattatgatacatttatataaataatatatatataaaaattaattatactattactttataatataataatatttatttataaagatataaaagaattgtttaaagttataactaaaatattatatagtattcattaataattaatattataaattcaactattgttatatttataaatagaataatatattattatcctttaagatataacaataattatttaaattaaattaaattaaatttaattaatttttttttttaatgaatataataataataatattattaaaattaatatataaaaaaaaagtaaaatactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809005_sequence/1
type: Sequence
persistent identity: BBa K809005 sequence
display id: BBa_K809005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaactcctaacggggttcccgcgaagcgggaactaataataatataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809201_sequence/1
type: Sequence
persistent identity: BBa K809201 sequence
display id: BBa_K809201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattccgaggactagaacactactgcaaagtaaaataccaattaccaggtactttgccagatgttgggcgcctcgcgtacgctacaacgtgtgccgtactctacccgcagcagcgttgcataccaatatcatcgcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809106_sequence/1
type: Sequence
persistent identity: BBa K809106 sequence
display id: BBa_K809106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaattactagagaaaactcctaacggggttcccgcgaagcgggaactaataataatataattactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809107_sequence/1
type: Sequence
persistent identity: BBa K809107 sequence
display id: BBa_K809107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaattactagagattatagttccggggcccggtcacggaagccggaaccccgcaaggagatttactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809403_sequence/1
type: Sequence
persistent identity: BBa K809403 sequence
display id: BBa_K809403_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgcttgtgaatgtgaacgcgttcacaagcgtatataagtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809009_sequence/1
type: Sequence
persistent identity: BBa K809009 sequence
display id: BBa_K809009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809007_sequence/1
type: Sequence
persistent identity: BBa K809007 sequence
display id: BBa_K809007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809501_sequence/1
type: Sequence
persistent identity: BBa K809501 sequence
display id: BBa_K809501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattccgaggactagaacactactgcaaagtaaaataccaattaccaggtactttgccagatgttgggcgcctcgcgtacgctacaacgtgtgccgtactctacccgcagcagcgttgcataccaatatcatcgcacattactagatggctactggtcgtgcccatcgccctgcaacccgatctcggggcattcccgaagcgacagttgctagattaccattgtatcttagggccctcacggcactatccgagcgttcagtcccgactgtatcgagtgaagagctggcggctgccgcaggagtgaatagcgcgaaattacgcaaggatttttcttacttggggtcctatggtacccgaggcgttggatacgacgtcgaatatcttgtataccaaatctcacgggagctcgggctaacacaggattggcctgtggttatagtcggtattggcaacctgggagctgccttagcaaattatgggggtttcgcgtcgagaggctttagggtagctgccttgatcgacgcagatcccggaatggcggggaaaccagtggctggtataccggttcaacacacggacgaacttgagaagattatccaggatgacggcgtcagtataggagtaattgccactccagcaggggcggctcaacaggtgtgtgatcgtctcgttgccgcaggtgtcaccagcatcctaaacttcgcgcccacagtactgaatgtgccagaaggcgttgacgtccgcaaagtagatttatctatagagttgcaaattcttgcttttcatgaacagcgaaaggccggagaggaagcagcggctgacggggccgcaccgcctgtggcggctcggaaacaacagagatccacgggttcagccgatcaaggccccgacggagatgttccagcagtcatgccggcgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809402_sequence/1
type: Sequence
persistent identity: BBa K809402 sequence
display id: BBa_K809402_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaatttgcttgtgaatgtgaacgcgttcacaagcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809108_sequence/1
type: Sequence
persistent identity: BBa K809108 sequence
display id: BBa_K809108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaattactagagaaaactcctaacggggttcccgcgaagcgggaactaataataatataattactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809002_sequence/1
type: Sequence
persistent identity: BBa K809002 sequence
display id: BBa_K809002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaatagggggagggggtgggtgataataaccagaatattcaataaatacagagcacacattacataaattttataatataaccaatataaaataaaattaaaataattaatatatatatataaatataataaattattatatataaatatatataatttttataataaatattataatattatataaataaataattataatatataataaatatataataataataaaaatattaacaatataataaaaatttataatataaatataaattataaataagttaaattaataaaataataaatgttaacaagaagatatctggggtcccattaataattattattttcaataataattgggaccccccaccattata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809001_sequence/1
type: Sequence
persistent identity: BBa K809001 sequence
display id: BBa_K809001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809503_sequence/1
type: Sequence
persistent identity: BBa K809503 sequence
display id: BBa_K809503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atataagtaatttgcttgtgaatgtgaacgcgttcacaagcgttactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataatactagagaaaactcctaacggggttcccgcgaagcgggaactaataataatataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809004_sequence/1
type: Sequence
persistent identity: BBa K809004 sequence
display id: BBa_K809004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attatagttccggggcccggtcacggaagccggaaccccgcaaggagatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K809401_sequence/1
type: Sequence
persistent identity: BBa K809401 sequence
display id: BBa_K809401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactggtcgtgcccatcgccctgcaacccgatctcggggcattcccgaagcgacagttgctagattaccattgtatcttagggccctcacggcactatccgagcgttcagtcccgactgtatcgagtgaagagctggcggctgccgcaggagtgaatagcgcgaaattacgcaaggatttttcttacttggggtcctatggtacccgaggcgttggatacgacgtcgaatatcttgtataccaaatctcacgggagctcgggctaacacaggattggcctgtggttatagtcggtattggcaacctgggagctgccttagcaaattatgggggtttcgcgtcgagaggctttagggtagctgccttgatcgacgcagatcccggaatggcggggaaaccagtggctggtataccggttcaacacacggacgaacttgagaagattatccaggatgacggcgtcagtataggagtaattgccactccagcaggggcggctcaacaggtgtgtgatcgtctcgttgccgcaggtgtcaccagcatcctaaacttcgcgcccacagtactgaatgtgccagaaggcgttgacgtccgcaaagtagatttatctatagagttgcaaattcttgcttttcatgaacagcgaaaggccggagaggaagcagcggctgacggggccgcaccgcctgtggcggctcggaaacaacagagatccacgggttcagccgatcaaggccccgacggagatgttccagcagtcatgccggcgtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z