//chassis/multihost
type: Collection
persistent identity: chassis multihost
display id: chassis_multihost
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSBBs2E
member: BBa_J179000
member: BBa_K1468001
member: pSBBs4S
member: BBa_K259009
member: BBa_J179001
member: pSBBs0K
member: spac+efe
member: pSBBs1C
member: Ateam
member: BBa_K1468000
member: BBa_J179002
member: BBa_K1468004
member: pTG262-BB
member: pSBBs3C
member: xyl+efe
member: BioScaffol
member: BBa_K801031
member: pSac-Cm_B
member: pSac-Cm_BB
member: BBa_K801035
member: OriV
member: BBa_K1468002
member: BBa_K125000
member: BBa_K259008
member: BBa_K801034

pSac-Cm_BB
type: ComponentDefinition
persistent identity: BBa K818000
display id: BBa_K818000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Emeraldo Jo
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1076_
m user id: 0
owner id: 11906
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The plasmid is designed to integrate a cloned insert into the B. subtilis 168 chromosome at the sacA locus. The user inserts the fragment inside the MCS whi
mutable notes: Biobrick ready
mutable provenance: MolGen Group (University of Groningen)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: BioBrick suffix
sequence annotation: sacA'
sequence annotation: BioBrick prefix
sequence annotation: cat
sequence annotation: BBa_B0015
sequence annotation: 'sacA
sequence: https://synbiohub.org/public/igem/BBa_K818000_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2203455
display id: annotation2203455
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1
type: Range
persistent identity: range2203455
display id: range2203455
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1324
end: 2196
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2203452
display id: annotation2203452
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1
type: Range
persistent identity: range2203452
display id: range2203452
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1
end: 17
orientation: inline

sacA'
type: SequenceAnnotation
persistent identity: annotation2203456
display id: annotation2203456
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1
type: Range
persistent identity: range2203456
display id: range2203456
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 3187
end: 3930
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2203458
display id: annotation2203458
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1
type: Range
persistent identity: range2203458
display id: range2203458
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 5207
end: 5223
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2203457
display id: annotation2203457
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1
type: Range
persistent identity: range2203457
display id: range2203457
version: 1
direction: direction/reverse
top level: pSac-Cm_BB
start: 4249
end: 4911
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2203453
display id: annotation2203453
version: 1
top level: pSac-Cm_BB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1
type: Range
persistent identity: range2203453
display id: range2203453
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 18
end: 170
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2203454
display id: annotation2203454
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1
type: Range
persistent identity: range2203454
display id: range2203454
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 181
end: 990
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823022
display id: BBa_K823022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S: Empty backbone for integration into Bacillus subtilis thrC locus
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.
mutable notes: This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.
mutable provenance: B.subtilis vector pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: hom
sequence annotation: ori approx.
sequence annotation: thrC'
sequence annotation: spc
sequence annotation: bla
sequence annotation: 'thrC
sequence annotation: thrB
sequence: https://synbiohub.org/public/igem/BBa_K823022_sequence/1

hom
type: SequenceAnnotation
persistent identity: annotation2179612
display id: annotation2179612
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1
type: Range
persistent identity: range2179612
display id: range2179612
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

ori approx.
type: SequenceAnnotation
persistent identity: annotation2179610
display id: annotation2179610
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1
type: Range
persistent identity: range2179610
display id: range2179610
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1942
end: 2556
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2179613
display id: annotation2179613
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1
type: Range
persistent identity: range2179613
display id: range2179613
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4542
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2179607
display id: annotation2179607
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1
type: Range
persistent identity: range2179607
display id: range2179607
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179611
display id: annotation2179611
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1
type: Range
persistent identity: range2179611
display id: range2179611
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2179608
display id: annotation2179608
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1
type: Range
persistent identity: range2179608
display id: range2179608
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2179609
display id: annotation2179609
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1
type: Range
persistent identity: range2179609
display id: range2179609
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

pSBBs0K
type: ComponentDefinition
persistent identity: BBa K823026
display id: BBa_K823026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid). This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pDG148
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacY
sequence annotation: ble
sequence annotation: repU
sequence annotation: kan
sequence annotation: bla
sequence annotation: lacI
sequence annotation: Pspac
sequence: https://synbiohub.org/public/igem/BBa_K823026_sequence/1

lacY
type: SequenceAnnotation
persistent identity: annotation2180261
display id: annotation2180261
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180261/range2180261/1
type: Range
persistent identity: range2180261
display id: range2180261
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 6906
end: 7871
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2180260
display id: annotation2180260
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180260/range2180260/1
type: Range
persistent identity: range2180260
display id: range2180260
version: 1
direction: direction/forward
top level: pSBBs0K
start: 6250
end: 6648
orientation: inline

repU
type: SequenceAnnotation
persistent identity: annotation2180258
display id: annotation2180258
version: 1
top level: pSBBs0K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180258/range2180258/1
type: Range
persistent identity: range2180258
display id: range2180258
version: 1
direction: direction/forward
top level: pSBBs0K
start: 4093
end: 5097
orientation: inline

kan
type: SequenceAnnotation
persistent identity: annotation2180259
display id: annotation2180259
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180259/range2180259/1
type: Range
persistent identity: range2180259
display id: range2180259
version: 1
direction: direction/forward
top level: pSBBs0K
start: 5257
end: 6027
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180257
display id: annotation2180257
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180257/range2180257/1
type: Range
persistent identity: range2180257
display id: range2180257
version: 1
direction: direction/reverse
top level: pSBBs0K
start: 2609
end: 3469
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2180256
display id: annotation2180256
version: 1
top level: pSBBs0K
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180256/range2180256/1
type: Range
persistent identity: range2180256
display id: range2180256
version: 1
direction: direction/forward
top level: pSBBs0K
start: 271
end: 1353
orientation: inline

Pspac
type: SequenceAnnotation
persistent identity: annotation2180262
display id: annotation2180262
version: 1
top level: pSBBs0K
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1

https://synbiohub.org/public/igem/BBa_K823026/annotation2180262/range2180262/1
type: Range
persistent identity: range2180262
display id: range2180262
version: 1
direction: direction/forward
top level: pSBBs0K
start: 8193
end: 8258
orientation: inline

BBa_J179002
type: ComponentDefinition
persistent identity: BBa J179002
display id: BBa_J179002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBs4S: Bacillus subtilis vector, thrC integration, spec resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. This backbone is a BioBricked version of the B. subtilis vector pDG1731.
mutable notes: This vector is compatible with pBS1C and pBS2E in one strain.
mutable provenance: This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox For handling of B. subtilis vectors, please see here. The transformation into B. subtilis is explained here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: thrC,thrB back
sequence annotation: ori
sequence annotation: hom, trC front
sequence annotation: bla
sequence annotation: spc
sequence: https://synbiohub.org/public/igem/BBa_J179002_sequence/1

thrC,thrB back
type: SequenceAnnotation
persistent identity: annotation2371767
display id: annotation2371767
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1
type: Range
persistent identity: range2371767
display id: range2371767
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1028
end: 1863
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371768
display id: annotation2371768
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1
type: Range
persistent identity: range2371768
display id: range2371768
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1942
end: 2556
orientation: inline

hom, trC front
type: SequenceAnnotation
persistent identity: annotation2371770
display id: annotation2371770
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1
type: Range
persistent identity: range2371770
display id: range2371770
version: 1
direction: direction/forward
top level: BBa_J179002
start: 3731
end: 4542
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371769
display id: annotation2371769
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1
type: Range
persistent identity: range2371769
display id: range2371769
version: 1
direction: direction/reverse
top level: BBa_J179002
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2371766
display id: annotation2371766
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1
type: Range
persistent identity: range2371766
display id: range2371766
version: 1
direction: direction/forward
top level: BBa_J179002
start: 211
end: 885
orientation: inline

BBa_K259009
type: ComponentDefinition
persistent identity: BBa K259009
display id: BBa_K259009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. This version is for when CspCI cuts 13/10b.p. (top/bottom) on the 5' of the recognition sequence and 10/13bp (top/bottom) on the 3' of the…
mutable notes: The CspCI discrepancy had to be taken into account
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 3' CspCI recognition sequence
sequence annotation: Translational Buffer
sequence annotation: BpuEI (rev. complement)
sequence annotation: 5' CspCI Recognition sequence
sequence annotation: BpuEI
sequence annotation: Met involved in restriction/ligation
sequence annotation: Tyr involved in restriction/ligation
sequence annotation: Gly-Ser-Gly linker
sequence: https://synbiohub.org/public/igem/BBa_K259009_sequence/1

3' CspCI recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037438
display id: annotation2037438
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037438/range2037438/1
type: Range
persistent identity: range2037438
display id: range2037438
version: 1
direction: direction/forward
top level: BBa_K259009
start: 74
end: 77
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037439
display id: annotation2037439
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037439/range2037439/1
type: Range
persistent identity: range2037439
display id: range2037439
version: 1
direction: direction/forward
top level: BBa_K259009
start: 69
end: 73
orientation: inline

BpuEI (rev. complement)
type: SequenceAnnotation
persistent identity: annotation2037400
display id: annotation2037400
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037400/range2037400/1
type: Range
persistent identity: range2037400
display id: range2037400
version: 1
direction: direction/reverse
top level: BBa_K259009
start: 3
end: 8
orientation: inline

5' CspCI Recognition sequence
type: SequenceAnnotation
persistent identity: annotation2037437
display id: annotation2037437
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037437/range2037437/1
type: Range
persistent identity: range2037437
display id: range2037437
version: 1
direction: direction/forward
top level: BBa_K259009
start: 66
end: 68
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037401
display id: annotation2037401
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037401/range2037401/1
type: Range
persistent identity: range2037401
display id: range2037401
version: 1
direction: direction/forward
top level: BBa_K259009
start: 21
end: 26
orientation: inline

Met involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037404
display id: annotation2037404
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037404/range2037404/1
type: Range
persistent identity: range2037404
display id: range2037404
version: 1
direction: direction/forward
top level: BBa_K259009
start: 53
end: 55
orientation: inline

Tyr involved in restriction/ligation
type: SequenceAnnotation
persistent identity: annotation2037402
display id: annotation2037402
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037402/range2037402/1
type: Range
persistent identity: range2037402
display id: range2037402
version: 1
direction: direction/forward
top level: BBa_K259009
start: 41
end: 43
orientation: inline

Gly-Ser-Gly linker
type: SequenceAnnotation
persistent identity: annotation2037403
display id: annotation2037403
version: 1
top level: BBa_K259009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037403/range2037403/1
type: Range
persistent identity: range2037403
display id: range2037403
version: 1
direction: direction/forward
top level: BBa_K259009
start: 44
end: 52
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037405
display id: annotation2037405
version: 1
top level: BBa_K259009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1

https://synbiohub.org/public/igem/BBa_K259009/annotation2037405/range2037405/1
type: Range
persistent identity: range2037405
display id: range2037405
version: 1
direction: direction/forward
top level: BBa_K259009
start: 56
end: 65
orientation: inline

BBa_J179000
type: ComponentDefinition
persistent identity: BBa J179000
display id: BBa_J179000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance
creator: Jara Radeck
created: 2013-12-03T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI. The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml). Before transformation into B. subtilis, this vector should be linearized with ScaI.
mutable notes: This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.
mutable provenance: pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori
sequence annotation: bla
sequence annotation: cat
sequence annotation: amyE-front
sequence annotation: amyE-back
sequence: https://synbiohub.org/public/igem/BBa_J179000_sequence/1

ori
type: SequenceAnnotation
persistent identity: annotation2371577
display id: annotation2371577
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1
type: Range
persistent identity: range2371577
display id: range2371577
version: 1
direction: direction/forward
top level: BBa_J179000
start: 3587
end: 4201
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371575
display id: annotation2371575
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1
type: Range
persistent identity: range2371575
display id: range2371575
version: 1
direction: direction/reverse
top level: BBa_J179000
start: 4361
end: 5221
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371573
display id: annotation2371573
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1
type: Range
persistent identity: range2371573
display id: range2371573
version: 1
direction: direction/forward
top level: BBa_J179000
start: 358
end: 1008
orientation: inline

amyE-front
type: SequenceAnnotation
persistent identity: annotation2371576
display id: annotation2371576
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1
type: Range
persistent identity: range2371576
display id: range2371576
version: 1
direction: direction/forward
top level: BBa_J179000
start: 5546
end: 6066
orientation: inline

amyE-back
type: SequenceAnnotation
persistent identity: annotation2371574
display id: annotation2371574
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1
type: Range
persistent identity: range2371574
display id: range2371574
version: 1
direction: direction/forward
top level: BBa_J179000
start: 1379
end: 2407
orientation: inline

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823024
display id: BBa_K823024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis</i
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus expression vector pXT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'thrC
sequence annotation: thrC'
sequence annotation: bla
sequence annotation: spc
sequence annotation: hom
sequence annotation: thrB
sequence annotation: Pxly region
sequence: https://synbiohub.org/public/igem/BBa_K823024_sequence/1

'thrC
type: SequenceAnnotation
persistent identity: annotation2180241
display id: annotation2180241
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180241/range2180241/1
type: Range
persistent identity: range2180241
display id: range2180241
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2180245
display id: annotation2180245
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180245/range2180245/1
type: Range
persistent identity: range2180245
display id: range2180245
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4572
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180243
display id: annotation2180243
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180243/range2180243/1
type: Range
persistent identity: range2180243
display id: range2180243
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2180240
display id: annotation2180240
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180240/range2180240/1
type: Range
persistent identity: range2180240
display id: range2180240
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2180244
display id: annotation2180244
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180244/range2180244/1
type: Range
persistent identity: range2180244
display id: range2180244
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2180242
display id: annotation2180242
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180242/range2180242/1
type: Range
persistent identity: range2180242
display id: range2180242
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

Pxly region
type: SequenceAnnotation
persistent identity: annotation2180246
display id: annotation2180246
version: 1
top level: pSBBs4S
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1

https://synbiohub.org/public/igem/BBa_K823024/annotation2180246/range2180246/1
type: Range
persistent identity: range2180246
display id: range2180246
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4539
end: 4763
orientation: inline

BBa_J179001
type: ComponentDefinition
persistent identity: BBa J179001
display id: BBa_J179001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.
mutable notes: Compatible with pBs1C and pBs4S in one strain.
mutable provenance: This backbone is a BioBricked version of the B. subtilis vector pAX01. This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11274134 H??rtl ''et al.'']
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: lacA back
sequence annotation: lacA front
sequence annotation: ori
sequence annotation: erm
sequence: https://synbiohub.org/public/igem/BBa_J179001_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2371762
display id: annotation2371762
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1
type: Range
persistent identity: range2371762
display id: range2371762
version: 1
direction: direction/forward
top level: BBa_J179001
start: 747
end: 1607
orientation: inline

lacA back
type: SequenceAnnotation
persistent identity: annotation2371761
display id: annotation2371761
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1
type: Range
persistent identity: range2371761
display id: range2371761
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 124
end: 531
orientation: inline

lacA front
type: SequenceAnnotation
persistent identity: annotation2371764
display id: annotation2371764
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1
type: Range
persistent identity: range2371764
display id: range2371764
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4078
end: 4593
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371763
display id: annotation2371763
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1
type: Range
persistent identity: range2371763
display id: range2371763
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 1758
end: 2381
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2371765
display id: annotation2371765
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1
type: Range
persistent identity: range2371765
display id: range2371765
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4891
end: 5628
orientation: inline

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259004
display id: BBa_K259004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein**
mutable notes: This part was designed taking into consideration the requirements by Assembly Standard 10 (RFC10).
mutable provenance: This part is completely designed ''de novo''. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Met Involved in Restriction/Ligation
sequence annotation: CspCI (5' Recognition Seq)
sequence annotation: BpuEI
sequence annotation: Translational Buffer
sequence annotation: CspCI (3' Recognition Seq)
sequence annotation: Tyr Involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259004_sequence/1

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014600
display id: annotation2014600
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014600/range2014600/1
type: Range
persistent identity: range2014600
display id: range2014600
version: 1
direction: direction/forward
top level: BioScaffol
start: 44
end: 52
orientation: inline

Met Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014601
display id: annotation2014601
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014601/range2014601/1
type: Range
persistent identity: range2014601
display id: range2014601
version: 1
direction: direction/forward
top level: BioScaffol
start: 53
end: 55
orientation: inline

CspCI (5' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014603
display id: annotation2014603
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014603/range2014603/1
type: Range
persistent identity: range2014603
display id: range2014603
version: 1
direction: direction/forward
top level: BioScaffol
start: 66
end: 68
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014598
display id: annotation2014598
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014598/range2014598/1
type: Range
persistent identity: range2014598
display id: range2014598
version: 1
direction: direction/forward
top level: BioScaffol
start: 21
end: 26
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014604
display id: annotation2014604
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014604/range2014604/1
type: Range
persistent identity: range2014604
display id: range2014604
version: 1
direction: direction/forward
top level: BioScaffol
start: 70
end: 72
orientation: inline

CspCI (3' Recognition Seq)
type: SequenceAnnotation
persistent identity: annotation2014605
display id: annotation2014605
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014605/range2014605/1
type: Range
persistent identity: range2014605
display id: range2014605
version: 1
direction: direction/forward
top level: BioScaffol
start: 74
end: 77
orientation: inline

Tyr Involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014599
display id: annotation2014599
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014599/range2014599/1
type: Range
persistent identity: range2014599
display id: range2014599
version: 1
direction: direction/forward
top level: BioScaffol
start: 41
end: 43
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2014597
display id: annotation2014597
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014597/range2014597/1
type: Range
persistent identity: range2014597
display id: range2014597
version: 1
direction: direction/reverse
top level: BioScaffol
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014602
display id: annotation2014602
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1

https://synbiohub.org/public/igem/BBa_K259004/annotation2014602/range2014602/1
type: Range
persistent identity: range2014602
display id: range2014602
version: 1
direction: direction/forward
top level: BioScaffol
start: 56
end: 65
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823023
display id: BBa_K823023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR.
mutable provenance: This backbone is an igem compatible version of the Bacillus vector pDG1662
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: amyE
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823023_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2180236
display id: annotation2180236
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1
type: Range
persistent identity: range2180236
display id: range2180236
version: 1
direction: direction/forward
top level: pSBBs1C
start: 358
end: 1008
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180239
display id: annotation2180239
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1
type: Range
persistent identity: range2180239
display id: range2180239
version: 1
direction: direction/forward
top level: pSBBs1C
start: 5546
end: 6066
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180238
display id: annotation2180238
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1
type: Range
persistent identity: range2180238
display id: range2180238
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 4361
end: 5221
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180237
display id: annotation2180237
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1
type: Range
persistent identity: range2180237
display id: range2180237
version: 1
direction: direction/forward
top level: pSBBs1C
start: 1379
end: 2407
orientation: inline

pSBBs3C
type: ComponentDefinition
persistent identity: BBa K823025
display id: BBa_K823025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
mutable provenance: This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: sacA
sequence annotation: luxC
sequence annotation: luxA
sequence annotation: cat
sequence annotation: bla
sequence annotation: luxE
sequence annotation: luxB
sequence annotation: luxD
sequence: https://synbiohub.org/public/igem/BBa_K823025_sequence/1

sacA
type: SequenceAnnotation
persistent identity: annotation2180252
display id: annotation2180252
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1
type: Range
persistent identity: range2180252
display id: range2180252
version: 1
direction: direction/forward
top level: pSBBs3C
start: 5676
end: 6515
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180254
display id: annotation2180254
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1
type: Range
persistent identity: range2180254
display id: range2180254
version: 1
direction: direction/forward
top level: pSBBs3C
start: 8712
end: 9503
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2180249
display id: annotation2180249
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1
type: Range
persistent identity: range2180249
display id: range2180249
version: 1
direction: direction/forward
top level: pSBBs3C
start: 2179
end: 3621
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2180247
display id: annotation2180247
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1
type: Range
persistent identity: range2180247
display id: range2180247
version: 1
direction: direction/forward
top level: pSBBs3C
start: 73
end: 1155
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180255
display id: annotation2180255
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1
type: Range
persistent identity: range2180255
display id: range2180255
version: 1
direction: direction/reverse
top level: pSBBs3C
start: 9661
end: 10323
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180253
display id: annotation2180253
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1
type: Range
persistent identity: range2180253
display id: range2180253
version: 1
direction: direction/forward
top level: pSBBs3C
start: 6849
end: 7721
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2180251
display id: annotation2180251
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1
type: Range
persistent identity: range2180251
display id: range2180251
version: 1
direction: direction/forward
top level: pSBBs3C
start: 4583
end: 5695
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2180248
display id: annotation2180248
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1
type: Range
persistent identity: range2180248
display id: range2180248
version: 1
direction: direction/forward
top level: pSBBs3C
start: 1172
end: 2155
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2180250
display id: annotation2180250
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1
type: Range
persistent identity: range2180250
display id: range2180250
version: 1
direction: direction/forward
top level: pSBBs3C
start: 3635
end: 4558
orientation: inline

pSac-Cm_B
type: ComponentDefinition
persistent identity: BBa K1185004
display id: BBa_K1185004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Vincent Leonardo
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid was derived from pSac-Cm by insertion of biobrick compatible restriction sites (prefixes and suffixes), a terminator (BBa_B0015) after the suffixes sequences, and red fluorescent protein sequence (RFP) in between the prefix and suffix in its multiple cloning sites (MCS). New biobricks can be inserted into this vector by replacement of the RFP biobrick, and selection of the white colonies. This backbone has a multi host replication origin and replicates in E. coli. The plasmid is designed to integrate a cloned insert into the B. subtilis chromosome via double recombination between plasmid and chromosomal sacA sequences, and in order to check for integration a simple Phenol Red Sucrose test can be perform, if sacA is no longer functional, they will remain ph7, if they are still able to digest sucrose their pH will be under 5.
mutable notes: We found that that the integration plasmid that groningen send to the registry were unable to integrate in B. subtilis. This is due to the high number of mutations in the sacA integration region.
mutable provenance: Groningen iGEM 2012 team
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'sacA
sequence annotation: B0015
sequence annotation: bla
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K1185004_sequence/1

'sacA
type: SequenceAnnotation
persistent identity: annotation2367616
display id: annotation2367616
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1
type: Range
persistent identity: range2367616
display id: range2367616
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 3167
end: 3922
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2367613
display id: annotation2367613
version: 1
top level: pSac-Cm_B
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1
type: Range
persistent identity: range2367613
display id: range2367613
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 23
end: 151
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2367615
display id: annotation2367615
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1
type: Range
persistent identity: range2367615
display id: range2367615
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 1306
end: 2176
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2367617
display id: annotation2367617
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1
type: Range
persistent identity: range2367617
display id: range2367617
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 4231
end: 4894
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2367614
display id: annotation2367614
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1
type: Range
persistent identity: range2367614
display id: range2367614
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 167
end: 970
orientation: inline

BBa_K801035
type: ComponentDefinition
persistent identity: BBa K801035
display id: BBa_K801035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-PIF3
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: composite part of Bba_K365005 and Bba_K365000
mutable notes: -
mutable provenance: composite part of Bba_K365005 and Bba_K365000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K365000
sequence annotation: RFC25 fusion scar
sequence annotation: T > A
sequence annotation: start
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801035_sequence/1

BBa_K365000
type: SequenceAnnotation
persistent identity: annotation2190058
display id: annotation2190058
version: 1
top level: BBa_K801035
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190058/range2190058/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190058/range2190058/1
type: Range
persistent identity: range2190058
display id: range2190058
version: 1
direction: direction/reverse
top level: BBa_K801035
start: 67
end: 366
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198904
display id: annotation2198904
version: 1
top level: BBa_K801035
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2198904/range2198904/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2198904/range2198904/1
type: Range
persistent identity: range2198904
display id: range2198904
version: 1
direction: direction/forward
top level: BBa_K801035
start: 61
end: 66
orientation: inline

T > A
type: SequenceAnnotation
persistent identity: annotation2190057
display id: annotation2190057
version: 1
top level: BBa_K801035
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190057/range2190057/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190057/range2190057/1
type: Range
persistent identity: range2190057
display id: range2190057
version: 1
direction: direction/forward
top level: BBa_K801035
start: 243
end: 243
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2190056
display id: annotation2190056
version: 1
top level: BBa_K801035
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190056/range2190056/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190056/range2190056/1
type: Range
persistent identity: range2190056
display id: range2190056
version: 1
direction: direction/forward
top level: BBa_K801035
start: 67
end: 69
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2190055
display id: annotation2190055
version: 1
top level: BBa_K801035
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190055/range2190055/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190055/range2190055/1
type: Range
persistent identity: range2190055
display id: range2190055
version: 1
direction: direction/reverse
top level: BBa_K801035
start: 1
end: 60
orientation: inline

OriV
type: ComponentDefinition
persistent identity: BBa K125340
display id: BBa_K125340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Origin of Vegetative Replication
creator: Margaret Ruzicka
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
mutable notes: This part is to be constructed with the Rep proteins derived from an RSF1010 plasmid.
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RepA Binding Site
sequence annotation: RepB Binding Site
sequence annotation: RepC Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K125340_sequence/1

RepA Binding Site
type: SequenceAnnotation
persistent identity: annotation1974216
display id: annotation1974216
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1
type: Range
persistent identity: range1974216
display id: range1974216
version: 1
direction: direction/forward
top level: OriV
start: 353
end: 401
orientation: inline

RepB Binding Site
type: SequenceAnnotation
persistent identity: annotation1974215
display id: annotation1974215
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1
type: Range
persistent identity: range1974215
display id: range1974215
version: 1
direction: direction/forward
top level: OriV
start: 207
end: 352
orientation: inline

RepC Binding Site
type: SequenceAnnotation
persistent identity: annotation1974214
display id: annotation1974214
version: 1
top level: OriV
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1

https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1
type: Range
persistent identity: range1974214
display id: range1974214
version: 1
direction: direction/forward
top level: OriV
start: 3
end: 66
orientation: inline

Ateam
type: ComponentDefinition
persistent identity: BBa K1023016
display id: BBa_K1023016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRET based ATP biosensor for expression in Aspergillus niger
creator: Shreyans Chordia
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1330_
m user id: 0
owner id: 15671
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Consists of cfp-yfp fret pair that responds to changes in ATP concentration
mutable notes: It has illegal restriction sites for the standard 10 assembly. The cds of the part is flanked by NdeI and NotI restriction sites.
mutable provenance: Imamura lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K1023002
sequence annotation: BBa_K1023001
sequence: https://synbiohub.org/public/igem/BBa_K1023016_sequence/1

BBa_K1023002
type: SequenceAnnotation
persistent identity: annotation2364482
display id: annotation2364482
version: 1
top level: Ateam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1023016/annotation2364482/range2364482/1

https://synbiohub.org/public/igem/BBa_K1023016/annotation2364482/range2364482/1
type: Range
persistent identity: range2364482
display id: range2364482
version: 1
direction: direction/forward
top level: Ateam
start: 711
end: 1546
orientation: inline

BBa_K1023001
type: SequenceAnnotation
persistent identity: annotation2364481
display id: annotation2364481
version: 1
top level: Ateam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1023016/annotation2364481/range2364481/1

https://synbiohub.org/public/igem/BBa_K1023016/annotation2364481/range2364481/1
type: Range
persistent identity: range2364481
display id: range2364481
version: 1
direction: direction/forward
top level: Ateam
start: 1
end: 702
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacZ
sequence annotation: START
sequence annotation: LacZ protein
sequence annotation: -10
sequence annotation: BBa_K1468004
sequence annotation: STOP
sequence annotation: TetR 2
sequence annotation: C --> T
sequence annotation: TetR 1
sequence annotation: BBa_R0040
sequence annotation: -35
sequence annotation: BBa_I732005
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_K801031
type: ComponentDefinition
persistent identity: BBa K801031
display id: BBa_K801031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochrome B (PhyB, first 908 N-terminal amino acids)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: improved version of BBa_K365002 to allow N- and C-terminal protein fusions using RFC 25
mutable notes: -
mutable provenance: Based on BBa_K365002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Essential Gly for photoconvertible binding of PIF3/6
sequence annotation: Essential Arg for photoconvertible binding of PIF3/6
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
sequence annotation: PhyB (2-908 AA) photosensitive core
sequence: https://synbiohub.org/public/igem/BBa_K801031_sequence/1

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198859
display id: annotation2198859
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
type: Range
persistent identity: range2198859
display id: range2198859
version: 1
direction: direction/forward
top level: BBa_K801031
start: 328
end: 330
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198856
display id: annotation2198856
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
type: Range
persistent identity: range2198856
display id: range2198856
version: 1
direction: direction/forward
top level: BBa_K801031
start: 325
end: 327
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198862
display id: annotation2198862
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
type: Range
persistent identity: range2198862
display id: range2198862
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1051
end: 1053
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
type: SequenceAnnotation
persistent identity: annotation2198864
display id: annotation2198864
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
type: Range
persistent identity: range2198864
display id: range2198864
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1066
end: 1068
orientation: inline

PhyB (2-908 AA) photosensitive core
type: SequenceAnnotation
persistent identity: annotation2343344
display id: annotation2343344
version: 1
top level: BBa_K801031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
type: Range
persistent identity: range2343344
display id: range2343344
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1
end: 2721
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198861
display id: annotation2198861
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
type: Range
persistent identity: range2198861
display id: range2198861
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1039
end: 1041
orientation: inline

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

spac+efe
type: ComponentDefinition
persistent identity: BBa K1065203
display id: BBa_K1065203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in pSpac (BBa_K823026)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pSpac vector that has benn sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly.
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1065002
component: BBa_K823026
sequence annotation: BBa_K1065002
sequence annotation: BBa_K823026
sequence: https://synbiohub.org/public/igem/BBa_K1065203_sequence/1

BBa_K1065002
type: Component
persistent identity: component2334235
display id: component2334235
version: 1
top level: spac+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823026
type: Component
persistent identity: component2334243
display id: component2334243
version: 1
top level: spac+efe
access: public
definition: pSBBs0K

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334235
display id: annotation2334235
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334235/range2334235/1
type: Range
persistent identity: range2334235
display id: range2334235
version: 1
direction: direction/reverse
top level: spac+efe
start: 1
end: 1215
orientation: inline

BBa_K823026
type: SequenceAnnotation
persistent identity: annotation2334243
display id: annotation2334243
version: 1
top level: spac+efe
location: https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
component: BBa_K823026

https://synbiohub.org/public/igem/BBa_K1065203/annotation2334243/range2334243/1
type: Range
persistent identity: range2334243
display id: range2334243
version: 1
direction: direction/reverse
top level: spac+efe
start: 1224
end: 9512
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823021
display id: BBa_K823021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: This reporter vector can be used with the normal BioBrick assembly strategies.
mutable provenance: This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: lacZ
sequence annotation: bla
sequence annotation: 'amyE
sequence annotation: amyE'
sequence: https://synbiohub.org/public/igem/BBa_K823021_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179606
display id: annotation2179606
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1
type: Range
persistent identity: range2179606
display id: range2179606
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 8813
end: 9463
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2179602
display id: annotation2179602
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1
type: Range
persistent identity: range2179602
display id: range2179602
version: 1
direction: direction/forward
top level: pSBBs1C
start: 49
end: 3112
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179604
display id: annotation2179604
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1
type: Range
persistent identity: range2179604
display id: range2179604
version: 1
direction: direction/forward
top level: pSBBs1C
start: 4023
end: 4883
orientation: inline

'amyE
type: SequenceAnnotation
persistent identity: annotation2179605
display id: annotation2179605
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1
type: Range
persistent identity: range2179605
display id: range2179605
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 7402
end: 8229
orientation: inline

amyE'
type: SequenceAnnotation
persistent identity: annotation2179603
display id: annotation2179603
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1
type: Range
persistent identity: range2179603
display id: range2179603
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 3165
end: 3698
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: START
sequence annotation: ZsGreeen
sequence annotation: BBa_K1468000
sequence annotation: BBa_E0040
sequence annotation: ZsGreen protein
sequence annotation: STOP
sequence annotation: BBa_J23104
sequence annotation: J23104p
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

pTG262-BB
type: ComponentDefinition
persistent identity: BBa I742123
display id: BBa_I742123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Multi-host vector pTG262 converted to BioBrick vector
creator: Sarah Hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: biobricked version of pTG262 Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.
mutable notes: n/a
mutable provenance: We kindly thank Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich for generously donating the pTG262 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repA
sequence annotation: cat
sequence annotation: BioBrick suffix
sequence annotation: neo
sequence annotation: BioBrick prefix
sequence: https://synbiohub.org/public/igem/BBa_I742123_sequence/1

repA
type: SequenceAnnotation
persistent identity: annotation1974395
display id: annotation1974395
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1
type: Range
persistent identity: range1974395
display id: range1974395
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1724
end: 2431
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation1974397
display id: annotation1974397
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1
type: Range
persistent identity: range1974397
display id: range1974397
version: 1
direction: direction/forward
top level: pTG262-BB
start: 4444
end: 5094
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974394
display id: annotation1974394
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1
type: Range
persistent identity: range1974394
display id: range1974394
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1
end: 21
orientation: inline

neo
type: SequenceAnnotation
persistent identity: annotation1974396
display id: annotation1974396
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1
type: Range
persistent identity: range1974396
display id: range1974396
version: 1
direction: direction/forward
top level: pTG262-BB
start: 3149
end: 3919
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974398
display id: annotation1974398
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1
type: Range
persistent identity: range1974398
display id: range1974398
version: 1
direction: direction/forward
top level: pTG262-BB
start: 5543
end: 5564
orientation: inline

BBa_K801034
type: ComponentDefinition
persistent identity: BBa K801034
display id: BBa_K801034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PIF3-linker
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: composite part of Bba_K365000 and Bba_K365005
mutable notes: -
mutable provenance: composite part of Bba_K365000 and Bba_K365005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: RFC25 fusion scar
sequence annotation: T > A
sequence annotation: BBa_K365000
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801034_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2198897
display id: annotation2198897
version: 1
top level: BBa_K801034
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198897/range2198897/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198897/range2198897/1
type: Range
persistent identity: range2198897
display id: range2198897
version: 1
direction: direction/forward
top level: BBa_K801034
start: 1
end: 3
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198901
display id: annotation2198901
version: 1
top level: BBa_K801034
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198901/range2198901/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198901/range2198901/1
type: Range
persistent identity: range2198901
display id: range2198901
version: 1
direction: direction/forward
top level: BBa_K801034
start: 301
end: 306
orientation: inline

T > A
type: SequenceAnnotation
persistent identity: annotation2198899
display id: annotation2198899
version: 1
top level: BBa_K801034
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198899/range2198899/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198899/range2198899/1
type: Range
persistent identity: range2198899
display id: range2198899
version: 1
direction: direction/forward
top level: BBa_K801034
start: 177
end: 177
orientation: inline

BBa_K365000
type: SequenceAnnotation
persistent identity: annotation2198898
display id: annotation2198898
version: 1
top level: BBa_K801034
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198898/range2198898/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198898/range2198898/1
type: Range
persistent identity: range2198898
display id: range2198898
version: 1
direction: direction/forward
top level: BBa_K801034
start: 1
end: 300
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198900
display id: annotation2198900
version: 1
top level: BBa_K801034
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198900/range2198900/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198900/range2198900/1
type: Range
persistent identity: range2198900
display id: range2198900
version: 1
direction: direction/forward
top level: BBa_K801034
start: 307
end: 366
orientation: inline

BBa_K259008
type: ComponentDefinition
persistent identity: BBa K259008
display id: BBa_K259008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions=== *Create an Assembly Standard 10 fusion as below ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part** *Use the BioScaffold Specific Enzymes (i)Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. (ii)Ligate and you will get the intermediate product; **Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein** *Restrict with CspCI to remove downstream scar. *Ligate to get the final product; **Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein** ====N.B.==== CspCI cuts with a 1 b.p discrepancy depending on the sequence. However this should not affect the restriction/ligation process as this has been taken into account and incorporated into the design. Even with the discrepancy involved the restriction/ligation process will still give the same product and in-frame fusion. The other restriction variants of CspCI have also been taken into account and relevant BioScaffolds are being produced to be tested. Updates will soon follow on this. This version accounts for cleaving DNA 12/10bp (top/bottom) on the 5' of the recognition sequence and 10…
mutable notes: CspCI enzyme has different variations in the distance of restricting DNA from the recognition site.This biobrick is almost identical to part BBa_K259004 but modified to account for this discrepancy.
mutable provenance: This part is completely designed de novo. This sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' CspCI recognition site
sequence annotation: 3' CspCI Recognition site
sequence annotation: Translational buffer
sequence annotation: Met involved in Restriction/Ligation
sequence annotation: BpuEI (rev.comp)
sequence annotation: BpuEI
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: Translational Buffer
sequence annotation: Tyr involved in Restriction/Ligation
sequence: https://synbiohub.org/public/igem/BBa_K259008_sequence/1

5' CspCI recognition site
type: SequenceAnnotation
persistent identity: annotation2037356
display id: annotation2037356
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037356/range2037356/1
type: Range
persistent identity: range2037356
display id: range2037356
version: 1
direction: direction/forward
top level: BBa_K259008
start: 65
end: 67
orientation: inline

3' CspCI Recognition site
type: SequenceAnnotation
persistent identity: annotation2037357
display id: annotation2037357
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037357/range2037357/1
type: Range
persistent identity: range2037357
display id: range2037357
version: 1
direction: direction/forward
top level: BBa_K259008
start: 73
end: 76
orientation: inline

Translational buffer
type: SequenceAnnotation
persistent identity: annotation2037358
display id: annotation2037358
version: 1
top level: BBa_K259008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037358/range2037358/1
type: Range
persistent identity: range2037358
display id: range2037358
version: 1
direction: direction/forward
top level: BBa_K259008
start: 68
end: 72
orientation: inline

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037354
display id: annotation2037354
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037354/range2037354/1
type: Range
persistent identity: range2037354
display id: range2037354
version: 1
direction: direction/forward
top level: BBa_K259008
start: 53
end: 55
orientation: inline

BpuEI (rev.comp)
type: SequenceAnnotation
persistent identity: annotation2037350
display id: annotation2037350
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037350/range2037350/1
type: Range
persistent identity: range2037350
display id: range2037350
version: 1
direction: direction/reverse
top level: BBa_K259008
start: 3
end: 8
orientation: inline

BpuEI
type: SequenceAnnotation
persistent identity: annotation2037352
display id: annotation2037352
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037352/range2037352/1
type: Range
persistent identity: range2037352
display id: range2037352
version: 1
direction: direction/forward
top level: BBa_K259008
start: 21
end: 26
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2037353
display id: annotation2037353
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037353/range2037353/1
type: Range
persistent identity: range2037353
display id: range2037353
version: 1
direction: direction/forward
top level: BBa_K259008
start: 44
end: 52
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2037355
display id: annotation2037355
version: 1
top level: BBa_K259008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037355/range2037355/1
type: Range
persistent identity: range2037355
display id: range2037355
version: 1
direction: direction/forward
top level: BBa_K259008
start: 56
end: 64
orientation: inline

Tyr involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2037351
display id: annotation2037351
version: 1
top level: BBa_K259008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1

https://synbiohub.org/public/igem/BBa_K259008/annotation2037351/range2037351/1
type: Range
persistent identity: range2037351
display id: range2037351
version: 1
direction: direction/forward
top level: BBa_K259008
start: 41
end: 43
orientation: inline

pSBBs2E
type: ComponentDefinition
persistent identity: BBa K823027
display id: BBa_K823027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus subtilis vector pAX01
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lacA'
sequence annotation: erm
sequence annotation: bla
sequence annotation: 'lacA
sequence: https://synbiohub.org/public/igem/BBa_K823027_sequence/1

lacA'
type: SequenceAnnotation
persistent identity: annotation2180264
display id: annotation2180264
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1
type: Range
persistent identity: range2180264
display id: range2180264
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 1600
end: 2100
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2180263
display id: annotation2180263
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1
type: Range
persistent identity: range2180263
display id: range2180263
version: 1
direction: direction/forward
top level: pSBBs2E
start: 500
end: 1300
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180265
display id: annotation2180265
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1
type: Range
persistent identity: range2180265
display id: range2180265
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 4603
end: 5400
orientation: inline

'lacA
type: SequenceAnnotation
persistent identity: annotation2180266
display id: annotation2180266
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1
type: Range
persistent identity: range2180266
display id: range2180266
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 5600
end: 6100
orientation: inline

BioScaffol
type: ComponentDefinition
persistent identity: BBa K259002
display id: BBa_K259002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
creator: Petros Mina
created: 2009-07-31T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is part of a family of Bioscaffold - Linkers. This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by: *Removing the stop codons from the upstream protein. *Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences. *Maintaining the start codon of the downstream protein. *Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences. *Acting as a translational buffer if ligation is incorrect. ===Assembly Instructions==== *Create an Assembly Standard 10 fusion giving ; **Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part *Use the BioScaffold Specific Enzymes **Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part. **Ligate and you will get the intermediate product; Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein **Restrict with BseRI to remove downstream scar. **Ligate to get the final product; Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein
mutable notes: Part was designed taking into account RFC10 assembly standard and as an attempt to be upwards compatible with RFC10.
mutable provenance: This part was designed ''de novo''. The sequence does not occur naturally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Met involved in Restriction/Ligation
sequence annotation: Tyr Residue involved in Restriction/Ligation
sequence annotation: BseRI Site
sequence annotation: Gly-Ser-Gly Linker
sequence annotation: BpuEI Site
sequence annotation: Translational Buffer
sequence: https://synbiohub.org/public/igem/BBa_K259002_sequence/1

Met involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014593
display id: annotation2014593
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014593/range2014593/1
type: Range
persistent identity: range2014593
display id: range2014593
version: 1
direction: direction/forward
top level: BioScaffol
start: 53
end: 55
orientation: inline

Tyr Residue involved in Restriction/Ligation
type: SequenceAnnotation
persistent identity: annotation2014589
display id: annotation2014589
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014589/range2014589/1
type: Range
persistent identity: range2014589
display id: range2014589
version: 1
direction: direction/forward
top level: BioScaffol
start: 41
end: 43
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014595
display id: annotation2014595
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014595/range2014595/1
type: Range
persistent identity: range2014595
display id: range2014595
version: 1
direction: direction/reverse
top level: BioScaffol
start: 63
end: 68
orientation: inline

Gly-Ser-Gly Linker
type: SequenceAnnotation
persistent identity: annotation2014592
display id: annotation2014592
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014592/range2014592/1
type: Range
persistent identity: range2014592
display id: range2014592
version: 1
direction: direction/forward
top level: BioScaffol
start: 44
end: 52
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014591
display id: annotation2014591
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014591/range2014591/1
type: Range
persistent identity: range2014591
display id: range2014591
version: 1
direction: direction/forward
top level: BioScaffol
start: 21
end: 26
orientation: inline

BseRI Site
type: SequenceAnnotation
persistent identity: annotation2014596
display id: annotation2014596
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014596/range2014596/1
type: Range
persistent identity: range2014596
display id: range2014596
version: 1
direction: direction/forward
top level: BioScaffol
start: 81
end: 86
orientation: inline

BpuEI Site
type: SequenceAnnotation
persistent identity: annotation2014590
display id: annotation2014590
version: 1
top level: BioScaffol
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014590/range2014590/1
type: Range
persistent identity: range2014590
display id: range2014590
version: 1
direction: direction/reverse
top level: BioScaffol
start: 3
end: 8
orientation: inline

Translational Buffer
type: SequenceAnnotation
persistent identity: annotation2014594
display id: annotation2014594
version: 1
top level: BioScaffol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1

https://synbiohub.org/public/igem/BBa_K259002/annotation2014594/range2014594/1
type: Range
persistent identity: range2014594
display id: range2014594
version: 1
direction: direction/forward
top level: BioScaffol
start: 56
end: 62
orientation: inline

xyl+efe
type: ComponentDefinition
persistent identity: BBa K1065204
display id: BBa_K1065204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Efe+Bba_B0015 in BBa_K823024 (pXyl)
creator: Viola Valentini
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1373_
m user id: 0
owner id: 15998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.
mutable notes: ===Design Notes=== The coding sequence was taken from Pseudomonas syringae pv. phaseolicola PK2. We firstly optimized the codons for both B. subtilis and E. coli and then synthesized the gene with an RBS sequence upstream of the start codon. We decided to include in the CDS two restriction sites (NgoMIV and AgeI) in order to allow people to use the Freiburg assembly. GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC... ...TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG
mutable provenance: This enzyme was firstly purified from Pseudomonas Siringae pv. phaseolicola PK2, a 2-oxoglutarate-dependent ethylene producing bacterium [1]. The CDS has been optimized for both E.coli and B.subtilis. We decided to include an RBS sequence too.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823024
component: BBa_K1065002
sequence annotation: BBa_K823024
sequence annotation: BBa_K1065002
sequence: https://synbiohub.org/public/igem/BBa_K1065204_sequence/1

BBa_K823024
type: Component
persistent identity: component2334267
display id: component2334267
version: 1
top level: xyl+efe
access: public
definition: pSBBs4S

BBa_K1065002
type: Component
persistent identity: component2334259
display id: component2334259
version: 1
top level: xyl+efe
access: public
definition: https://synbiohub.org/public/igem/BBa_K1065002/1

BBa_K823024
type: SequenceAnnotation
persistent identity: annotation2334267
display id: annotation2334267
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
component: BBa_K823024

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334267/range2334267/1
type: Range
persistent identity: range2334267
display id: range2334267
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1224
end: 6017
orientation: inline

BBa_K1065002
type: SequenceAnnotation
persistent identity: annotation2334259
display id: annotation2334259
version: 1
top level: xyl+efe
location: https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
component: BBa_K1065002

https://synbiohub.org/public/igem/BBa_K1065204/annotation2334259/range2334259/1
type: Range
persistent identity: range2334259
display id: range2334259
version: 1
direction: direction/reverse
top level: xyl+efe
start: 1
end: 1215
orientation: inline

Ateam
type: ComponentDefinition
persistent identity: BBa K1023003
display id: BBa_K1023003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRET based ATP biosensor cds, codon optimized for Aspergillus niger
creator: Shreyans Chordia (shreyanschordia90@gmail.com)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1330_
m user id: 0
owner id: 15730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A FRET based biosensor that will measure the ratio of ATP and ADP.
mutable provenance: Aspergillus niger
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1023003_sequence/1

BBa_K125000
type: ComponentDefinition
persistent identity: BBa K125000
display id: BBa_K125000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Broad host range BioBrick plasmid derived from pRL1383a with low to medium copy number
creator: Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RSF1010 is a broad-host-range plasmid first described in 1974 (Guerry 1974), with its entire sequence and gene organization subsequently described in 1989 (Scholz 1989). It is a naturally occurring 8.6kb broad-host-range plasmid in the E. coli incompatibility group Q. The conjugative transfer and stable replication of this plasmid are possible due to the mob genes with the associated origin of transfer (oriT) and the rep genes with the associated origin of vegetative replication (oriV), respectively. RSF1010 derived plasmids which include the oriV and associated Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, and Serratia (Leemans 1987). In addition, RSF1010 derived plasmids including the oriV, oriT and its associated rep and mob genes are transferred by conjugation to at least four cyanobacteria strains (Mermet-Bouvier 1993). These cyanobacteria strains include Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301. A number of RSF1010 derived plasmids have been constructed due to the utility of this broad-host-range plasmid. For example, pSB2A, containing a 5.6kb RSF1010 derived region including the necessary mobilization and replication regions, can be transferred through conjugation to and stably maintained in Synechocystis PCC6803, PCC6714, and Synechococcus PCC7942 and PCC6301 (Marraccini 1993). The RSF1010 plasmid used in this study, pRL1383a was constructed for use in genomic studies of the diazotrophic, multicellular cyanobacterium Anabaena PCC 7120. This plasmid contains the mob and rep regions necessary for conjugation and autonomous replication, respectively. Additionally this vector is made resistant to Streptomycin and Spectinomycin due to the presence of the aadA gene (Wolk 2007). This plasmid can be mobilized by the E. coli derived self-transmissible plasmid RP4. Mobilization genes are not necessary for transfer of a mobilizable plasmid if the self-transmissible pl…
mutable notes: The original pRL1383a MCS has been replaced by the BioBrick BBa_B0034 and its flanking BioBrick restriction sites.
mutable provenance: Plasmid is derived from pRL1383a.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: spectinomycin marker
sequence annotation: VF2
sequence annotation: T4 32 terminator
sequence annotation: VR
sequence annotation: loxP
sequence annotation: BioBrick prefix
sequence annotation: P5 promoter
sequence annotation: RSF1010 derived region
sequence annotation: oriR
sequence annotation: oriT
sequence annotation: T7 promoter
sequence annotation: omega interposon
sequence annotation: cosN
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K125000_sequence/1

spectinomycin marker
type: SequenceAnnotation
persistent identity: annotation1996995
display id: annotation1996995
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1
type: Range
persistent identity: range1996995
display id: range1996995
version: 1
direction: direction/forward
top level: BBa_K125000
start: 933
end: 1943
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1969229
display id: annotation1969229
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1
type: Range
persistent identity: range1969229
display id: range1969229
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8908
end: 8927
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999636
display id: annotation1999636
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1
type: Range
persistent identity: range1999636
display id: range1999636
version: 1
direction: direction/forward
top level: BBa_K125000
start: 343
end: 374
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1969228
display id: annotation1969228
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1
type: Range
persistent identity: range1969228
display id: range1969228
version: 1
direction: direction/reverse
top level: BBa_K125000
start: 78
end: 97
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation1999635
display id: annotation1999635
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1
type: Range
persistent identity: range1999635
display id: range1999635
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8831
end: 8864
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999639
display id: annotation1999639
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1
type: Range
persistent identity: range1999639
display id: range1999639
version: 1
direction: direction/forward
top level: BBa_K125000
start: 9026
end: 9047
orientation: inline

P5 promoter
type: SequenceAnnotation
persistent identity: annotation1969227
display id: annotation1969227
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1
type: Range
persistent identity: range1969227
display id: range1969227
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8200
end: 8230
orientation: inline

RSF1010 derived region
type: SequenceAnnotation
persistent identity: annotation1969582
display id: annotation1969582
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1
type: Range
persistent identity: range1969582
display id: range1969582
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2664
end: 8356
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999637
display id: annotation1999637
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1
type: Range
persistent identity: range1999637
display id: range1999637
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2249
end: 2280
orientation: inline

oriR
type: SequenceAnnotation
persistent identity: annotation1969581
display id: annotation1969581
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1
type: Range
persistent identity: range1969581
display id: range1969581
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2819
end: 3243
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation1969230
display id: annotation1969230
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1
type: Range
persistent identity: range1969230
display id: range1969230
version: 1
direction: direction/forward
top level: BBa_K125000
start: 3476
end: 3633
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999633
display id: annotation1999633
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1
type: Range
persistent identity: range1999633
display id: range1999633
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8880
end: 8898
orientation: inline

omega interposon
type: SequenceAnnotation
persistent identity: annotation1969583
display id: annotation1969583
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1
type: Range
persistent identity: range1969583
display id: range1969583
version: 1
direction: direction/forward
top level: BBa_K125000
start: 463
end: 1255
orientation: inline

cosN
type: SequenceAnnotation
persistent identity: annotation1999634
display id: annotation1999634
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1
type: Range
persistent identity: range1999634
display id: range1999634
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8616
end: 8705
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999638
display id: annotation1999638
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1
type: Range
persistent identity: range1999638
display id: range1999638
version: 1
direction: direction/forward
top level: BBa_K125000
start: 1
end: 21
orientation: inline