//chassis/miscellaneous
type: Collection
persistent identity: chassis miscellaneous
display id: chassis_miscellaneous
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I723011
member: BBa_I735010
member: pFe
member: BBa_K624000
member: BBa_J64931
member: BBa_K260018
member: BBa_I723013
member: BBa_J24001
member: BBa_J48103
member: BBa_I735008
member: BBa_K624004
member: BBa_J49000
member: BBa_J16400
member: BBa_J24000
member: BBa_J48106
member: Pr
member: BBa_I735009
member: BBa_I748001
member: BBa_J54110
member: BBa_K112126
member: BBa_K112907
member: BBa_K624003
member: BBa_K624005
member: BBa_J61045

BBa_J61045
type: ComponentDefinition
persistent identity: BBa J61045
display id: BBa_J61045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [spv] spv operon (PoPS out)
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61045_sequence/1

BBa_J54110
type: ComponentDefinition
persistent identity: BBa J54110
display id: BBa_J54110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MelR_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:11Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter
mutable notes: as MelR promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: MelR_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54110_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903810
display id: annotation1903810
version: 1
top level: BBa_J54110
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54110/annotation1903810/range1903810/1

https://synbiohub.org/public/igem/BBa_J54110/annotation1903810/range1903810/1
type: Range
persistent identity: range1903810
display id: range1903810
version: 1
direction: direction/forward
top level: BBa_J54110
start: 1
end: 5
orientation: inline

MelR_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903811
display id: annotation1903811
version: 1
top level: BBa_J54110
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54110/annotation1903811/range1903811/1

https://synbiohub.org/public/igem/BBa_J54110/annotation1903811/range1903811/1
type: Range
persistent identity: range1903811
display id: range1903811
version: 1
direction: direction/forward
top level: BBa_J54110
start: 6
end: 74
orientation: inline

BBa_I748001
type: ComponentDefinition
persistent identity: BBa I748001
display id: BBa_I748001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Putative Cyanide Nitrilase Promoter
creator: Kurt Whittemore
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
group u list: _149_
m user id: 0
owner id: 1654
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This section of DNA contains the sequence from 300 base pairs upstream of the Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase gene to 30 base pairs downstream into the gene from Pseudomonas fluorescens PfO-1. Our hope is that this region of DNA will contain the promoter for the nitrilase gene. At the time that this part was submitted, it was not tested or sequenced yet.
mutable notes: We isolated this segment of DNA from the Pseudomonas fluorescens PfO-1 genome using a PCR reaction.
mutable provenance: This section of DNA comes from the Pseudomonas fluorescens PfO-1 genome (NC_007492). The GeneID for the nitrilase gene that we working with is 3715134, and the protein ID for the nitrilase gene is YP_349417.1. We took the region 300 base pairs upstream of the nitrilase gene and 10 base pairs downstream into the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I748001_sequence/1

BBa_I723013
type: ComponentDefinition
persistent identity: BBa I723013
display id: BBa_I723013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pDntA (estimated promoter for DntA)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter activated by the transcriptional regulator DntR [BB# here] in response to detection of environmental [salicylate].
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pDntA
sequence: https://synbiohub.org/public/igem/BBa_I723013_sequence/1

pDntA
type: SequenceAnnotation
persistent identity: annotation1954960
display id: annotation1954960
version: 1
top level: BBa_I723013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723013/annotation1954960/range1954960/1

https://synbiohub.org/public/igem/BBa_I723013/annotation1954960/range1954960/1
type: Range
persistent identity: range1954960
display id: range1954960
version: 1
direction: direction/forward
top level: BBa_I723013
start: 1
end: 33
orientation: inline

BBa_K624000
type: ComponentDefinition
persistent identity: BBa K624000
display id: BBa_K624000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ori+Pmsp1
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: express
mutable notes: express
mutable provenance: express
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ori (pMGT)
sequence annotation: Pmsp1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K624000_sequence/1

ori (pMGT)
type: SequenceAnnotation
persistent identity: annotation2148112
display id: annotation2148112
version: 1
top level: BBa_K624000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148112/range2148112/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148112/range2148112/1
type: Range
persistent identity: range2148112
display id: range2148112
version: 1
direction: direction/forward
top level: BBa_K624000
start: 1
end: 80
orientation: inline

Pmsp1
type: SequenceAnnotation
persistent identity: annotation2148113
display id: annotation2148113
version: 1
top level: BBa_K624000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148113/range2148113/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148113/range2148113/1
type: Range
persistent identity: range2148113
display id: range2148113
version: 1
direction: direction/forward
top level: BBa_K624000
start: 81
end: 274
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148114
display id: annotation2148114
version: 1
top level: BBa_K624000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K624000/annotation2148114/range2148114/1

https://synbiohub.org/public/igem/BBa_K624000/annotation2148114/range2148114/1
type: Range
persistent identity: range2148114
display id: range2148114
version: 1
direction: direction/forward
top level: BBa_K624000
start: 275
end: 277
orientation: inline

BBa_K260018
type: ComponentDefinition
persistent identity: BBa K260018
display id: BBa_K260018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cell free Chassis: Mass production of aqueous vesicles in oil phase
creator: Deepikaa Menon and Tomasz Sadowski
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _356_
m user id: 0
owner id: 4384
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: true
mutable description: Method to mass produce vesicles of uniform shape and volume Vesicle characterization Content of the vesicles can be chosen freely. Environment inside vesicles is aquious. Vesicle shape: spherical Vesicle diameter: 15 ??m Vesicle volume: ~12 pl Vesicle population has a very narrow distribution in size. Production efficiency: 5-15 vesicles per second Vesicle stability: not fully characterized, depends on surfactant used, at least 2 hours; fusion of vesicles observed after >24 hours Optical properties: transparent in brightfield microscopy; for observation of fluorescence the surfactant used should be non-fluorescent Vesicle isolation through output hole. Vesicle content is released when vesicles transfered to aqueous Protocol You will need a silicone waver with the engraved microfluidic system, and a micropump system Setup of the microfluidic system The microfluidic system consists of a flow chamber made of Polydimethylsiloxane (PDMS) and a pump system that controls the flow rates of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows containment and imaging. Two types of chambers have been used, differing in the geometry of the space where droplets were produced. One featured T-junction, and the other a V-junction. Production of flow chambers: * mix PDMS and curing agent in 10:1 ratio * degas and pour on wafer with etched microstructures * polymerize on heat plate at 150 ??C for 30 min * add unpolymerized PDMS mixture to points on microstructure where microtube inlets are to be pierced * polymerize on heat plate at 150 ??C for 30 minutes * remove polymerized PDMS from wafer, cut to fit onto glass cover slide (24 x 60 mm), and use clean needles (0.8 mm) or laser cutter (Trotec Speedy 100TM) to pierce tube inlets * ionize PDMS and glass slide in plasma chamber for 30 sec to make it reactive * align PDMS on glass slide and seal * seal irreversibly by heating on plate at 60??C for 6 …
mutable notes: does not apply. part contains no sequence
mutable provenance: does not apply. part contains no sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110

BBa_K624005
type: ComponentDefinition
persistent identity: BBa K624005
display id: BBa_K624005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mms13
creator: Shang-Jui Tsai
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _796_
m user id: 0
owner id: 9029
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: magnetite tight-binding transmembrane protein interfacing magnetic field and mechanobiology.
mutable notes: fit with biobrick assembly rules
mutable provenance: PCR product from Magnetospirillum magneticum AMB-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: mms13
sequence: https://synbiohub.org/public/igem/BBa_K624005_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2148126
display id: annotation2148126
version: 1
top level: BBa_K624005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K624005/annotation2148126/range2148126/1

https://synbiohub.org/public/igem/BBa_K624005/annotation2148126/range2148126/1
type: Range
persistent identity: range2148126
display id: range2148126
version: 1
direction: direction/forward
top level: BBa_K624005
start: 373
end: 375
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148125
display id: annotation2148125
version: 1
top level: BBa_K624005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K624005/annotation2148125/range2148125/1

https://synbiohub.org/public/igem/BBa_K624005/annotation2148125/range2148125/1
type: Range
persistent identity: range2148125
display id: range2148125
version: 1
direction: direction/forward
top level: BBa_K624005
start: 1
end: 3
orientation: inline

mms13
type: SequenceAnnotation
persistent identity: annotation2148124
display id: annotation2148124
version: 1
top level: BBa_K624005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K624005/annotation2148124/range2148124/1

https://synbiohub.org/public/igem/BBa_K624005/annotation2148124/range2148124/1
type: Range
persistent identity: range2148124
display id: range2148124
version: 1
direction: direction/forward
top level: BBa_K624005
start: 4
end: 372
orientation: inline

BBa_K112907
type: ComponentDefinition
persistent identity: BBa K112907
display id: BBa_K112907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcon
creator: Bing Xia
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A constituitive promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112907_sequence/1

BBa_J49000
type: ComponentDefinition
persistent identity: BBa J49000
display id: BBa_J49000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Roid Rage
creator: Andrew Sichel
created: 2006-06-14T11:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _74_
m user id: 0
owner id: 1008
owning group id: 74
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Anger and ACNE
mutable notes: needs to be injected
mutable provenance: a sketchy alley
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J49000_sequence/1

BBa_J24000
type: ComponentDefinition
persistent identity: BBa J24000
display id: BBa_J24000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CafAp (Cafeine Dependant promoter)
creator: John Cumbers
created: 2006-06-13T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 604
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stimulated by CafA found in cafein addicted students
mutable notes: Also activated by sucrose Inhibited by nicotine
mutable provenance: Student genome sequencing project
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: strong
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_J24000_sequence/1

strong
type: SequenceAnnotation
persistent identity: annotation1880709
display id: annotation1880709
version: 1
top level: BBa_J24000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J24000/annotation1880709/range1880709/1

https://synbiohub.org/public/igem/BBa_J24000/annotation1880709/range1880709/1
type: Range
persistent identity: range1880709
display id: range1880709
version: 1
direction: direction/reverse
top level: BBa_J24000
start: 1
end: 13
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation1880714
display id: annotation1880714
version: 1
top level: BBa_J24000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J24000/annotation1880714/range1880714/1

https://synbiohub.org/public/igem/BBa_J24000/annotation1880714/range1880714/1
type: Range
persistent identity: range1880714
display id: range1880714
version: 1
direction: direction/reverse
top level: BBa_J24000
start: 1
end: 10
orientation: inline

BBa_J48103
type: ComponentDefinition
persistent identity: BBa J48103
display id: BBa_J48103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Iron promoter
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Encodes for oxidoreductase and promotes the reduction/oxidation of iron (FeII and FeII).
mutable notes: Digested using Pst I, Xba I inserted into BBa_E0422.
mutable provenance: Genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48103_sequence/1

BBa_I735008
type: ComponentDefinition
persistent identity: BBa I735008
display id: BBa_I735008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ORE1X Oleate response element
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG
mutable notes: No considerations!
mutable provenance: We amplificated this part from p2UG2XORE plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735008_sequence/1

BBa_K112126
type: ComponentDefinition
persistent identity: BBa K112126
display id: BBa_K112126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAH57 promoter
creator: Christina Brown
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
mutable notes: none
mutable provenance: cloned from E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112126_sequence/1

BBa_K624004
type: ComponentDefinition
persistent identity: BBa K624004
display id: BBa_K624004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ori+Pmsp1+rep (pYMB essentials)
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: express in AMB-1
mutable notes: Express in AMB-1
mutable provenance: Express in AMB-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K624003
component: BBa_K624000
sequence annotation: BBa_K624003
sequence annotation: BBa_K624000
sequence: https://synbiohub.org/public/igem/BBa_K624004_sequence/1

BBa_K624003
type: Component
persistent identity: component2148123
display id: component2148123
version: 1
top level: BBa_K624004
access: public
definition: BBa_K624003

BBa_K624000
type: Component
persistent identity: component2148120
display id: component2148120
version: 1
top level: BBa_K624004
access: public
definition: BBa_K624000

BBa_K624003
type: SequenceAnnotation
persistent identity: annotation2148123
display id: annotation2148123
version: 1
top level: BBa_K624004
location: https://synbiohub.org/public/igem/BBa_K624004/annotation2148123/range2148123/1
component: BBa_K624003

https://synbiohub.org/public/igem/BBa_K624004/annotation2148123/range2148123/1
type: Range
persistent identity: range2148123
display id: range2148123
version: 1
direction: direction/reverse
top level: BBa_K624004
start: 286
end: 1142
orientation: inline

BBa_K624000
type: SequenceAnnotation
persistent identity: annotation2148120
display id: annotation2148120
version: 1
top level: BBa_K624004
location: https://synbiohub.org/public/igem/BBa_K624004/annotation2148120/range2148120/1
component: BBa_K624000

https://synbiohub.org/public/igem/BBa_K624004/annotation2148120/range2148120/1
type: Range
persistent identity: range2148120
display id: range2148120
version: 1
direction: direction/reverse
top level: BBa_K624004
start: 1
end: 277
orientation: inline

pFe
type: ComponentDefinition
persistent identity: BBa I765000
display id: BBa_I765000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe promoter
creator: Raul Cuero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter regulated by iron presence
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1956037
display id: annotation1956037
version: 1
top level: pFe
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1

https://synbiohub.org/public/igem/BBa_I765000/annotation1956037/range1956037/1
type: Range
persistent identity: range1956037
display id: range1956037
version: 1
direction: direction/forward
top level: pFe
start: 1
end: 1045
orientation: inline

BBa_J64931
type: ComponentDefinition
persistent identity: BBa J64931
display id: BBa_J64931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnKp promoter
creator: Kristopher Kuchenbecker
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Natural promoter for glnK (-150 to 0)
mutable notes: n/a
mutable provenance: ''E. coli'' K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64931_sequence/1

Pr
type: ComponentDefinition
persistent identity: BBa I723018
display id: BBa_I723018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr (promoter for XylR)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
mutable notes: [Christine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pr
sequence: https://synbiohub.org/public/igem/BBa_I723018_sequence/1

Pr
type: SequenceAnnotation
persistent identity: annotation1955191
display id: annotation1955191
version: 1
top level: Pr
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1

https://synbiohub.org/public/igem/BBa_I723018/annotation1955191/range1955191/1
type: Range
persistent identity: range1955191
display id: range1955191
version: 1
direction: direction/forward
top level: Pr
start: 1
end: 410
orientation: inline

BBa_I735009
type: ComponentDefinition
persistent identity: BBa I735009
display id: BBa_I735009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ORE2X oleate response element
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG. In this case we have 2 ore consensus sequences.
mutable notes: Downstream to ORE sequence there is CYC TATA
mutable provenance: We amplified this part from p2UG2XORE plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735009_sequence/1

BBa_J16400
type: ComponentDefinition
persistent identity: BBa J16400
display id: BBa_J16400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Iron sensitive promoter (test delete later)
creator: Andrew Hessel
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 341
owning group id: 67
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a promoter that is sensitive to iron (Fe++) concentration (test only)
mutable notes: made a base substitution to remove a EcoRI site
mutable provenance: Sequence obtained from GB accn J00010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: binding region
sequence: https://synbiohub.org/public/igem/BBa_J16400_sequence/1

binding region
type: SequenceAnnotation
persistent identity: annotation1883622
display id: annotation1883622
version: 1
top level: BBa_J16400
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J16400/annotation1883622/range1883622/1

https://synbiohub.org/public/igem/BBa_J16400/annotation1883622/range1883622/1
type: Range
persistent identity: range1883622
display id: range1883622
version: 1
direction: direction/forward
top level: BBa_J16400
start: 11
end: 16
orientation: inline

BBa_I723011
type: ComponentDefinition
persistent identity: BBa I723011
display id: BBa_I723011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pDntR (estimated promoter for DntR)
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring (possibly stationary-phase only) promoter for the DntR gene (which encodes the transcriptional regulator DntR) back-to-back with the DntR-regulated promoter.
mutable notes: This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pDntR
sequence: https://synbiohub.org/public/igem/BBa_I723011_sequence/1

pDntR
type: SequenceAnnotation
persistent identity: annotation1955001
display id: annotation1955001
version: 1
top level: BBa_I723011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723011/annotation1955001/range1955001/1

https://synbiohub.org/public/igem/BBa_I723011/annotation1955001/range1955001/1
type: Range
persistent identity: range1955001
display id: range1955001
version: 1
direction: direction/reverse
top level: BBa_I723011
start: 1
end: 26
orientation: inline

BBa_J24001
type: ComponentDefinition
persistent identity: BBa J24001
display id: BBa_J24001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: WigLp (Wiggle-dependent Promotor)
creator: Jamie Lemon
created: 2006-06-13T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1016
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Is stimulated by wiggle movement.
mutable notes: Is inhibited by lethergy
mutable provenance: Fictional
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: WigLp-a
sequence: https://synbiohub.org/public/igem/BBa_J24001_sequence/1

WigLp-a
type: SequenceAnnotation
persistent identity: annotation1880710
display id: annotation1880710
version: 1
top level: BBa_J24001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J24001/annotation1880710/range1880710/1

https://synbiohub.org/public/igem/BBa_J24001/annotation1880710/range1880710/1
type: Range
persistent identity: range1880710
display id: range1880710
version: 1
direction: direction/forward
top level: BBa_J24001
start: 1
end: 22
orientation: inline

BBa_J48106
type: ComponentDefinition
persistent identity: BBa J48106
display id: BBa_J48106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vnfH
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Vanadium promoter
mutable notes: cut with Xba I and Pst I
mutable provenance: nitrogenase iron protein, vnfH Rhodopseudomonas palustris CGA009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48106_sequence/1

BBa_K624003
type: ComponentDefinition
persistent identity: BBa K624003
display id: BBa_K624003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rep gene
creator: SHANG-JUI TSAI, CHUN-YU LAI
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _796_
m user id: 0
owner id: 6664
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: only the plasmid contain rep gene can be used in magnetic bacteria AMB-1
mutable notes: yes
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: rep (pMGT)
sequence annotation: rbs from pMGT
sequence: https://synbiohub.org/public/igem/BBa_K624003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2148127
display id: annotation2148127
version: 1
top level: BBa_K624003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148127/range2148127/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148127/range2148127/1
type: Range
persistent identity: range2148127
display id: range2148127
version: 1
direction: direction/forward
top level: BBa_K624003
start: 25
end: 27
orientation: inline

rep (pMGT)
type: SequenceAnnotation
persistent identity: annotation2148115
display id: annotation2148115
version: 1
top level: BBa_K624003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148115/range2148115/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148115/range2148115/1
type: Range
persistent identity: range2148115
display id: range2148115
version: 1
direction: direction/forward
top level: BBa_K624003
start: 28
end: 857
orientation: inline

rbs from pMGT
type: SequenceAnnotation
persistent identity: annotation2148116
display id: annotation2148116
version: 1
top level: BBa_K624003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K624003/annotation2148116/range2148116/1

https://synbiohub.org/public/igem/BBa_K624003/annotation2148116/range2148116/1
type: Range
persistent identity: range2148116
display id: range2148116
version: 1
direction: direction/forward
top level: BBa_K624003
start: 1
end: 24
orientation: inline

BBa_I735010
type: ComponentDefinition
persistent identity: BBa I735010
display id: BBa_I735010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This promoter encoding for a thiolase involved in beta-oxidation of fatty acids.
creator: Giovanni Russo
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _139_
m user id: 0
owner id: 1571
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This promoter contains one only ora consensus sequence that respond to oleic acid. Its sequence is CGGN3TNA/(R)N8-12CCG
mutable notes: No considerations!
mutable provenance: We amplified this part from genomic DNA yeast.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I735010_sequence/1