//chassis/eukaryote/yeast
type: Collection
persistent identity: chassis eukaryote yeast
display id: chassis_eukaryote_yeast
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K110016
member: BBa_K1486034
member: BBa_I766214
member: ABF1
member: BBa_K792011
member: BBa_K165093
member: BBa_K1592000
member: BBa_K1314017
member: BBa_K801070
member: BBa_K106672
member: BBa_K775003
member: BBa_K1680019
member: BBa_K801040
member: mCherry
member: BBa_K110004
member: BBa_K106697
member: HisTag
member: BBa_K1125002
member: Cripto-1
member: BBa_K394003
member: BBa_K1486029
member: BBa_K1680007
member: BBa_K284002
member: BBa_Y00073
member: BBa_K1680024
member: BBa_K1871002
member: TrpZipper2
member: BBa_K284003
member: BBa_K124000
member: HBsAg
member: BBa_K950003
member: BBa_K1680012
member: V0120
member: BBa_K1680025
member: BBa_K863200
member: BBa_K801071
member: BBa_K165022
member: BBa_K1680013
member: BBa_K950004
member: BBa_K976009
member: BBa_K165052
member: mOrange
member: BBa_K165050
member: BBa_K165057
member: BBa_K149001
member: Kozak & st
member: BBa_K1486033
member: BBa_K801011
member: BBa_K1680006
member: MlrA
member: BBa_K392005
member: BBa_K1907008
member: BBa_K801095
member: BBa_K165016
member: Sin3
member: BBa_K165033
member: BBa_K165082
member: lexA
member: BBa_K165076
member: BBa_K165046
member: BBa_K165059
member: BBa_K165089
member: BBa_K1936000
member: BBa_K1728020
member: BBa_K211002
member: BBa_M36527
member: BBa_K105029
member: BBa_K392003
member: BBa_K976008
member: BBa_K1680017
member: tADH1
member: BBa_K165086

cin8
type: ComponentDefinition
persistent identity: BBa K105013
display id: BBa_K105013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cin8 - cell cycle specific degradation tag in yeast
creator: Robin Sorg
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick is the xxx domain of the yeast cin8 protein. It has been shown that this region of part of the protein is suffient to get xxx specific degradation via the APC complex.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: KEN-box
sequence: https://synbiohub.org/public/igem/BBa_K105013_sequence/1

KEN-box
type: SequenceAnnotation
persistent identity: annotation1983852
display id: annotation1983852
version: 1
top level: cin8
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K105013/annotation1983852/range1983852/1

https://synbiohub.org/public/igem/BBa_K105013/annotation1983852/range1983852/1
type: Range
persistent identity: range1983852
display id: range1983852
version: 1
direction: direction/forward
top level: cin8
start: 63
end: 81
orientation: inline

BBa_K1861070
type: ComponentDefinition
persistent identity: BBa K1861070
display id: BBa_K1861070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE3 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T03:37:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861701
component: BBa_K1159201
component: BBa_K1861102
component: BBa_K1861104
component: BBa_K1861101
component: BBa_K392003
sequence annotation: BBa_K1861701
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861101
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159201
sequence: https://synbiohub.org/public/igem/BBa_K1861070_sequence/1

BBa_K1861701
type: Component
persistent identity: component2474670
display id: component2474670
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861701/1

BBa_K1159201
type: Component
persistent identity: component2474673
display id: component2474673
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861102
type: Component
persistent identity: component2474669
display id: component2474669
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861104
type: Component
persistent identity: component2474674
display id: component2474674
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861101
type: Component
persistent identity: component2474668
display id: component2474668
version: 1
top level: BBa_K1861070
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K392003
type: Component
persistent identity: component2474675
display id: component2474675
version: 1
top level: BBa_K1861070
access: public
definition: BBa_K392003

BBa_K1861701
type: SequenceAnnotation
persistent identity: annotation2474670
display id: annotation2474670
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474670/range2474670/1
component: BBa_K1861701

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474670/range2474670/1
type: Range
persistent identity: range2474670
display id: range2474670
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 266
end: 2480
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474669
display id: annotation2474669
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474669/range2474669/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474669/range2474669/1
type: Range
persistent identity: range2474669
display id: range2474669
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 239
end: 265
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474674
display id: annotation2474674
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474674/range2474674/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474674/range2474674/1
type: Range
persistent identity: range2474674
display id: range2474674
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2520
end: 2528
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474668
display id: annotation2474668
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474668/range2474668/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474668/range2474668/1
type: Range
persistent identity: range2474668
display id: range2474668
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 1
end: 238
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474675
display id: annotation2474675
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474675/range2474675/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474675/range2474675/1
type: Range
persistent identity: range2474675
display id: range2474675
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2529
end: 2657
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474673
display id: annotation2474673
version: 1
top level: BBa_K1861070
location: https://synbiohub.org/public/igem/BBa_K1861070/annotation2474673/range2474673/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861070/annotation2474673/range2474673/1
type: Range
persistent identity: range2474673
display id: range2474673
version: 1
direction: direction/reverse
top level: BBa_K1861070
start: 2481
end: 2519
orientation: inline

BBa_K319005
type: ComponentDefinition
persistent identity: BBa K319005
display id: BBa_K319005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast mid-length ADH1 promoter
creator: Afnan Azizi
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:11:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the full length ADH1 promoter including the distal and proximal portions and their regulatory elements.
mutable notes: There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.
mutable provenance: The part was extracted from S. cerevisiae, YPH500 strain, using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene according to SGD sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ADH1 promoter
sequence: https://synbiohub.org/public/igem/BBa_K319005_sequence/1

ADH1 promoter
type: SequenceAnnotation
persistent identity: annotation2112269
display id: annotation2112269
version: 1
top level: BBa_K319005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K319005/annotation2112269/range2112269/1

https://synbiohub.org/public/igem/BBa_K319005/annotation2112269/range2112269/1
type: Range
persistent identity: range2112269
display id: range2112269
version: 1
direction: direction/forward
top level: BBa_K319005
start: 1
end: 720
orientation: inline

BBa_K1728020
type: ComponentDefinition
persistent identity: BBa K1728020
display id: BBa_K1728020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rhodopsin signaling peptide (bovine)
creator: Wan-Yun Chiu, Ming-Fei Hsieh
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T01:15:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2149_
m user id: 18379
owner id: 18379
owning group id: 9
status: status/Planning
bookmark: false
mutable description: rhodopsin signaling peptide (bovine)
mutable notes: No
mutable provenance: Order the DNA part from synthesis company
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1728020_sequence/1

BBa_K416000
type: ComponentDefinition
persistent identity: BBa K416000
display id: BBa_K416000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2 CDS Responsible for Yeast Surface Display
creator: Russell Durrett
created: 2010-05-27T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell. The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions.
mutable notes: We removed the STOP codon to allow for N- and C-terminal fusions, which have both been proven to occur efficiently.
mutable provenance: This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Aga2 CDS
sequence: https://synbiohub.org/public/igem/BBa_K416000_sequence/1

Aga2 CDS
type: SequenceAnnotation
persistent identity: annotation2069499
display id: annotation2069499
version: 1
top level: BBa_K416000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K416000/annotation2069499/range2069499/1

https://synbiohub.org/public/igem/BBa_K416000/annotation2069499/range2069499/1
type: Range
persistent identity: range2069499
display id: range2069499
version: 1
direction: direction/forward
top level: BBa_K416000
start: 1
end: 261
orientation: inline

BBa_K106693
type: ComponentDefinition
persistent identity: BBa K106693
display id: BBa_K106693
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a special variant acceptor vector for AarI cloning. The "A" site used here, termed "A!" is an alternate end used when no amino-terminal fusions are tolerated. Please see the UCSF 2008 iGEM wiki for more information on AarI cloning. This was designed specifically for use with Sir3 and amino-terminal domains of Sir3.
mutable notes: Note that this vector will NOT accept standard AarI "A" parts.
mutable provenance: Modified pRS315 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampicillin resistance marker
sequence annotation: Adh1 terminator
sequence annotation: ampicillin resistance promoter
sequence annotation: pBR322 origin
sequence annotation: lacZalpha reporter
sequence annotation: Start codon
sequence annotation: AarI A!D MCS
sequence annotation: T3 promoter
sequence annotation: Adh1 promoter
sequence annotation: Adh1P! promoter
sequence annotation: LEU2 marker
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: T7 promoter
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: Stop codons in all 3 frames
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106693_sequence/1

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1994690
display id: annotation1994690
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1
type: Range
persistent identity: range1994690
display id: range1994690
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4053
end: 4911
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1994693
display id: annotation1994693
version: 1
top level: BBa_K106693
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1
type: Range
persistent identity: range1994693
display id: range1994693
version: 1
direction: direction/forward
top level: BBa_K106693
start: 18
end: 211
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999719
display id: annotation1999719
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1
type: Range
persistent identity: range1999719
display id: range1999719
version: 1
direction: direction/forward
top level: BBa_K106693
start: 3983
end: 4011
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999720
display id: annotation1999720
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1
type: Range
persistent identity: range1999720
display id: range1999720
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5068
end: 5687
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999717
display id: annotation1999717
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1
type: Range
persistent identity: range1999717
display id: range1999717
version: 1
direction: direction/forward
top level: BBa_K106693
start: 246
end: 405
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999726
display id: annotation1999726
version: 1
top level: BBa_K106693
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1
type: Range
persistent identity: range1999726
display id: range1999726
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7638
end: 7640
orientation: inline

AarI A!D MCS
type: SequenceAnnotation
persistent identity: annotation1994692
display id: annotation1994692
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1
type: Range
persistent identity: range1994692
display id: range1994692
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4305
end: 4381
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999722
display id: annotation1999722
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1
type: Range
persistent identity: range1999722
display id: range1999722
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6095
end: 6114
orientation: inline

Adh1 promoter
type: SequenceAnnotation
persistent identity: annotation1999723
display id: annotation1999723
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1
type: Range
persistent identity: range1999723
display id: range1999723
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7229
end: 7625
orientation: inline

Adh1P! promoter
type: SequenceAnnotation
persistent identity: annotation1994691
display id: annotation1994691
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1
type: Range
persistent identity: range1994691
display id: range1994691
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6132
end: 7642
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1994688
display id: annotation1994688
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1
type: Range
persistent identity: range1994688
display id: range1994688
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1570
end: 2676
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999721
display id: annotation1999721
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1
type: Range
persistent identity: range1999721
display id: range1999721
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999725
display id: annotation1999725
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1
type: Range
persistent identity: range1999725
display id: range1999725
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7647
end: 7650
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999716
display id: annotation1999716
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1
type: Range
persistent identity: range1999716
display id: range1999716
version: 1
direction: direction/forward
top level: BBa_K106693
start: 224
end: 242
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999718
display id: annotation1999718
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1
type: Range
persistent identity: range1999718
display id: range1999718
version: 1
direction: direction/forward
top level: BBa_K106693
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999724
display id: annotation1999724
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1
type: Range
persistent identity: range1999724
display id: range1999724
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1
end: 4
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999727
display id: annotation1999727
version: 1
top level: BBa_K106693
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1
type: Range
persistent identity: range1999727
display id: range1999727
version: 1
direction: direction/forward
top level: BBa_K106693
start: 11
end: 21
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1994689
display id: annotation1994689
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1
type: Range
persistent identity: range1994689
display id: range1994689
version: 1
direction: direction/reverse
top level: BBa_K106693
start: 3403
end: 3921
orientation: inline

BBa_K801037
type: ComponentDefinition
persistent identity: BBa K801037
display id: BBa_K801037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-GAL4 DNA binding domain
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K365005 BBa_K801032
mutable notes: -
mutable provenance: Composite part of BBa_K365005 BBa_K801032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K801032
sequence annotation: BBa_K365005
sequence annotation: RFC25 fusion scar
sequence: https://synbiohub.org/public/igem/BBa_K801037_sequence/1

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2198906
display id: annotation2198906
version: 1
top level: BBa_K801037
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198906/range2198906/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198906/range2198906/1
type: Range
persistent identity: range2198906
display id: range2198906
version: 1
direction: direction/reverse
top level: BBa_K801037
start: 67
end: 504
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198905
display id: annotation2198905
version: 1
top level: BBa_K801037
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198905/range2198905/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198905/range2198905/1
type: Range
persistent identity: range2198905
display id: range2198905
version: 1
direction: direction/reverse
top level: BBa_K801037
start: 1
end: 60
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198908
display id: annotation2198908
version: 1
top level: BBa_K801037
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801037/annotation2198908/range2198908/1

https://synbiohub.org/public/igem/BBa_K801037/annotation2198908/range2198908/1
type: Range
persistent identity: range2198908
display id: range2198908
version: 1
direction: direction/forward
top level: BBa_K801037
start: 61
end: 66
orientation: inline

BBa_Y1015
type: ComponentDefinition
persistent identity: BBa Y1015
display id: BBa_Y1015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CycE1
creator: Samantha Sutton
created: 2005-06-22T11:00:00Z
modified: 2015-05-08T01:14:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 351
owning group id: 43
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_Y1015_sequence/1

V0120
type: ComponentDefinition
persistent identity: BBa J63010
display id: BBa_J63010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein fusion vector (Silver lab standard)
creator: Ira Phillips and Caroline Ajo-Franklin
created: 2006-10-17T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: standard biofusion plasmid containing ccdB death gene and chlorenphenicol resistance
mutable notes: transform into TOP10 survival
mutable provenance: synthetized DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: beta-lactamase (bla gene)
sequence annotation: incorrect silver fusion prefix
sequence annotation: RNAI transcript
sequence annotation: f1 origin
sequence annotation: modified BioBrick suffix
sequence annotation: lacZ alpha peptide
sequence annotation: modified BioBrick prefix
sequence annotation: RNAII transcript
sequence annotation: rep pMB1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_J63010_sequence/1

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1908165
display id: annotation1908165
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1
type: Range
persistent identity: range1908165
display id: range1908165
version: 1
direction: direction/reverse
top level: V0120
start: 1253
end: 2113
orientation: inline

incorrect silver fusion prefix
type: SequenceAnnotation
persistent identity: annotation2172657
display id: annotation2172657
version: 1
top level: V0120
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1

https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1
type: Range
persistent identity: range2172657
display id: range2172657
version: 1
direction: direction/forward
top level: V0120
start: 3260
end: 3260
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969671
display id: annotation1969671
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1
type: Range
persistent identity: range1969671
display id: range1969671
version: 1
direction: direction/forward
top level: V0120
start: 936
end: 1043
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1969668
display id: annotation1969668
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1
type: Range
persistent identity: range1969668
display id: range1969668
version: 1
direction: direction/forward
top level: V0120
start: 2632
end: 2938
orientation: inline

modified BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1908166
display id: annotation1908166
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1
type: Range
persistent identity: range1908166
display id: range1908166
version: 1
direction: direction/forward
top level: V0120
start: 1
end: 20
orientation: inline

lacZ alpha peptide
type: SequenceAnnotation
persistent identity: annotation1969669
display id: annotation1969669
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1
type: Range
persistent identity: range1969669
display id: range1969669
version: 1
direction: direction/reverse
top level: V0120
start: 3028
end: 3187
orientation: inline

modified BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969672
display id: annotation1969672
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1
type: Range
persistent identity: range1969672
display id: range1969672
version: 1
direction: direction/forward
top level: V0120
start: 3246
end: 3266
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969670
display id: annotation1969670
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1
type: Range
persistent identity: range1969670
display id: range1969670
version: 1
direction: direction/reverse
top level: V0120
start: 494
end: 1046
orientation: inline

rep pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1908167
display id: annotation1908167
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1
type: Range
persistent identity: range1908167
display id: range1908167
version: 1
direction: direction/forward
top level: V0120
start: 479
end: 1093
orientation: inline

BBa_K165082
type: ComponentDefinition
persistent identity: BBa K165082
display id: BBa_K165082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMET25 + Untagged LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165020
component: BBa_K165000
sequence annotation: BBa_K165002
sequence annotation: BBa_K165000
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165082_sequence/1

BBa_K165002
type: Component
persistent identity: component1997140
display id: component1997140
version: 1
top level: BBa_K165082
access: public
definition: BBa_K165002

BBa_K165020
type: Component
persistent identity: component1997141
display id: component1997141
version: 1
top level: BBa_K165082
access: public
definition: lexA

BBa_K165000
type: Component
persistent identity: component1997139
display id: component1997139
version: 1
top level: BBa_K165082
access: public
definition: BBa_K165000

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997140
display id: annotation1997140
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997140/range1997140/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165082/annotation1997140/range1997140/1
type: Range
persistent identity: range1997140
display id: range1997140
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 396
end: 413
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997139
display id: annotation1997139
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997139/range1997139/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165082/annotation1997139/range1997139/1
type: Range
persistent identity: range1997139
display id: range1997139
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 1
end: 387
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997141
display id: annotation1997141
version: 1
top level: BBa_K165082
location: https://synbiohub.org/public/igem/BBa_K165082/annotation1997141/range1997141/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165082/annotation1997141/range1997141/1
type: Range
persistent identity: range1997141
display id: range1997141
version: 1
direction: direction/reverse
top level: BBa_K165082
start: 422
end: 1093
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165000
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165000

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_K801001
type: ComponentDefinition
persistent identity: BBa K801001
display id: BBa_K801001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM101 yeast shuttle vector with pTEF1 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible
mutable provenance: Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 micron (??) origin
sequence annotation: CYC1-Terminator
sequence annotation: URA3
sequence annotation: T7-Promoter
sequence annotation: TEF1-Promoter
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801001_sequence/1

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201773
display id: annotation2201773
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1
type: Range
persistent identity: range2201773
display id: range2201773
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 3777
end: 5248
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2201769
display id: annotation2201769
version: 1
top level: BBa_K801001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1
type: Range
persistent identity: range2201769
display id: range2201769
version: 1
direction: direction/forward
top level: BBa_K801001
start: 540
end: 787
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201772
display id: annotation2201772
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1
type: Range
persistent identity: range2201772
display id: range2201772
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 2666
end: 3773
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201767
display id: annotation2201767
version: 1
top level: BBa_K801001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1
type: Range
persistent identity: range2201767
display id: range2201767
version: 1
direction: direction/forward
top level: BBa_K801001
start: 4
end: 23
orientation: inline

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2201768
display id: annotation2201768
version: 1
top level: BBa_K801001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1
type: Range
persistent identity: range2201768
display id: range2201768
version: 1
direction: direction/forward
top level: BBa_K801001
start: 58
end: 505
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201770
display id: annotation2201770
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1
type: Range
persistent identity: range2201770
display id: range2201770
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 970
end: 1643
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201771
display id: annotation2201771
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1
type: Range
persistent identity: range2201771
display id: range2201771
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 1788
end: 2647
orientation: inline

BBa_K801064
type: ComponentDefinition
persistent identity: BBa K801064
display id: BBa_K801064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express ''Citrus limon'' (+)-limonene synthase 1 in yeast. The expression is controled by the yeast TEF2 promoter and the yeast CYC1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF2 promoter (BBa_K801010), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K122003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K801061
sequence annotation: Cyc1 terminator
sequence annotation: stop codon
sequence annotation: Coding sequence
sequence annotation: TEF2 promotor
sequence annotation: Age1 restriction site
sequence: https://synbiohub.org/public/igem/BBa_K801064_sequence/1

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197887
display id: annotation2197887
version: 1
top level: BBa_K801064
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197887/range2197887/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197887/range2197887/1
type: Range
persistent identity: range2197887
display id: range2197887
version: 1
direction: direction/reverse
top level: BBa_K801064
start: 655
end: 2319
orientation: inline

Cyc1 terminator
type: SequenceAnnotation
persistent identity: annotation2197890
display id: annotation2197890
version: 1
top level: BBa_K801064
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197890/range2197890/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197890/range2197890/1
type: Range
persistent identity: range2197890
display id: range2197890
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2336
end: 2618
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2197892
display id: annotation2197892
version: 1
top level: BBa_K801064
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197892/range2197892/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197892/range2197892/1
type: Range
persistent identity: range2197892
display id: range2197892
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2326
end: 2328
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197886
display id: annotation2197886
version: 1
top level: BBa_K801064
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197886/range2197886/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197886/range2197886/1
type: Range
persistent identity: range2197886
display id: range2197886
version: 1
direction: direction/forward
top level: BBa_K801064
start: 655
end: 2319
orientation: inline

TEF2 promotor
type: SequenceAnnotation
persistent identity: annotation2197889
display id: annotation2197889
version: 1
top level: BBa_K801064
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197889/range2197889/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197889/range2197889/1
type: Range
persistent identity: range2197889
display id: range2197889
version: 1
direction: direction/forward
top level: BBa_K801064
start: 1
end: 649
orientation: inline

Age1 restriction site
type: SequenceAnnotation
persistent identity: annotation2197891
display id: annotation2197891
version: 1
top level: BBa_K801064
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197891/range2197891/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197891/range2197891/1
type: Range
persistent identity: range2197891
display id: range2197891
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2320
end: 2325
orientation: inline

Adh1P Acce
type: ComponentDefinition
persistent identity: BBa K106006
display id: BBa_K106006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Adh1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Adh1 promoter (Adh1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.
mutable provenance: A modified pRS315 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stops in all 3 frames
sequence annotation: T7 promoter
sequence annotation: Adh1 Promoter
sequence annotation: pBR322 origin
sequence annotation: ampicillin resistance marker
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: T3 promoter
sequence annotation: AarI AD MCS
sequence annotation: CEN ARS
sequence annotation: lac promoter
sequence annotation: Adh1 terminator
sequence annotation: A overhang
sequence annotation: LEU2 marker
sequence annotation: ampicillin resistance promoter
sequence annotation: ATG start codon
sequence annotation: lacZalpha reporter
sequence: https://synbiohub.org/public/igem/BBa_K106006_sequence/1

Stops in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999672
display id: annotation1999672
version: 1
top level: Adh1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1
type: Range
persistent identity: range1999672
display id: range1999672
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999674
display id: annotation1999674
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1
type: Range
persistent identity: range1999674
display id: range1999674
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 224
end: 242
orientation: inline

Adh1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986773
display id: annotation1986773
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1
type: Range
persistent identity: range1986773
display id: range1986773
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6132
end: 7646
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999678
display id: annotation1999678
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1
type: Range
persistent identity: range1999678
display id: range1999678
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5068
end: 5687
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986805
display id: annotation1986805
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1
type: Range
persistent identity: range1986805
display id: range1986805
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4053
end: 4911
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999676
display id: annotation1999676
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1
type: Range
persistent identity: range1999676
display id: range1999676
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999682
display id: annotation1999682
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1
type: Range
persistent identity: range1999682
display id: range1999682
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1
end: 4
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999680
display id: annotation1999680
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1
type: Range
persistent identity: range1999680
display id: range1999680
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6095
end: 6114
orientation: inline

AarI AD MCS
type: SequenceAnnotation
persistent identity: annotation1986789
display id: annotation1986789
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1
type: Range
persistent identity: range1986789
display id: range1986789
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4309
end: 4385
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986790
display id: annotation1986790
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1
type: Range
persistent identity: range1986790
display id: range1986790
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 3403
end: 3921
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999679
display id: annotation1999679
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1
type: Range
persistent identity: range1999679
display id: range1999679
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5996
end: 6025
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986788
display id: annotation1986788
version: 1
top level: Adh1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1
type: Range
persistent identity: range1986788
display id: range1986788
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 22
end: 215
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999681
display id: annotation1999681
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1
type: Range
persistent identity: range1999681
display id: range1999681
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7647
end: 7650
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986791
display id: annotation1986791
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1
type: Range
persistent identity: range1986791
display id: range1986791
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1570
end: 2676
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999677
display id: annotation1999677
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1
type: Range
persistent identity: range1999677
display id: range1999677
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 3983
end: 4011
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999673
display id: annotation1999673
version: 1
top level: Adh1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1
type: Range
persistent identity: range1999673
display id: range1999673
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7638
end: 7640
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999675
display id: annotation1999675
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1
type: Range
persistent identity: range1999675
display id: range1999675
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 246
end: 405
orientation: inline

BBa_K863200
type: ComponentDefinition
persistent identity: BBa K863200
display id: BBa_K863200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 5' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: coming soon
mutable provenance: K. pastoris
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863200_sequence/1

BBa_K976004
type: ComponentDefinition
persistent identity: BBa K976004
display id: BBa_K976004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbA2 promoter, 5
creator: Amna Sohail & Molly Petrous & Tyler Knox & Mackenzie Reetz
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a E. coli promoter + Luciferase Composite part.
mutable notes: No specific design considerations were dealt with.
mutable provenance: NCBI GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I712019
component: BBa_K390017
sequence annotation: BBa_I712019
sequence annotation: BBa_K390017
sequence: https://synbiohub.org/public/igem/BBa_K976004_sequence/1

BBa_I712019
type: Component
persistent identity: component2329807
display id: component2329807
version: 1
top level: BBa_K976004
access: public
definition: https://synbiohub.org/public/igem/BBa_I712019/1

BBa_K390017
type: Component
persistent identity: component2329806
display id: component2329806
version: 1
top level: BBa_K976004
access: public
definition: https://synbiohub.org/public/igem/BBa_K390017/1

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2329807
display id: annotation2329807
version: 1
top level: BBa_K976004
location: https://synbiohub.org/public/igem/BBa_K976004/annotation2329807/range2329807/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K976004/annotation2329807/range2329807/1
type: Range
persistent identity: range2329807
display id: range2329807
version: 1
direction: direction/reverse
top level: BBa_K976004
start: 94
end: 1746
orientation: inline

BBa_K390017
type: SequenceAnnotation
persistent identity: annotation2329806
display id: annotation2329806
version: 1
top level: BBa_K976004
location: https://synbiohub.org/public/igem/BBa_K976004/annotation2329806/range2329806/1
component: BBa_K390017

https://synbiohub.org/public/igem/BBa_K976004/annotation2329806/range2329806/1
type: Range
persistent identity: range2329806
display id: range2329806
version: 1
direction: direction/reverse
top level: BBa_K976004
start: 1
end: 87
orientation: inline

yfp
type: ComponentDefinition
persistent identity: BBa K165005
display id: BBa_K165005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus YFP, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the venus YFP, yeast optimized in the Silver Lab BioFusion standard for fusion of protein domains.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K165005_sequence/1

BBa_K801063
type: ComponentDefinition
persistent identity: BBa K801063
display id: BBa_K801063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express Citrus limon (+)-limonene synthase 1 in yeast. The expression is controled by the yeast TEF1 promoter and the yeast TEF1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Age1 restriction site
sequence annotation: Stop codon
sequence annotation: Coding sequence
sequence annotation: BBa_K801061
sequence annotation: TEF1 promotor
sequence annotation: TEF1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K801063_sequence/1

Age1 restriction site
type: SequenceAnnotation
persistent identity: annotation2197875
display id: annotation2197875
version: 1
top level: BBa_K801063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197875/range2197875/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197875/range2197875/1
type: Range
persistent identity: range2197875
display id: range2197875
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2127
end: 2132
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2197864
display id: annotation2197864
version: 1
top level: BBa_K801063
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197864/range2197864/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197864/range2197864/1
type: Range
persistent identity: range2197864
display id: range2197864
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2133
end: 2135
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197849
display id: annotation2197849
version: 1
top level: BBa_K801063
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197849/range2197849/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197849/range2197849/1
type: Range
persistent identity: range2197849
display id: range2197849
version: 1
direction: direction/forward
top level: BBa_K801063
start: 462
end: 2126
orientation: inline

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197850
display id: annotation2197850
version: 1
top level: BBa_K801063
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197850/range2197850/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197850/range2197850/1
type: Range
persistent identity: range2197850
display id: range2197850
version: 1
direction: direction/forward
top level: BBa_K801063
start: 462
end: 2126
orientation: inline

TEF1 promotor
type: SequenceAnnotation
persistent identity: annotation2197858
display id: annotation2197858
version: 1
top level: BBa_K801063
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197858/range2197858/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197858/range2197858/1
type: Range
persistent identity: range2197858
display id: range2197858
version: 1
direction: direction/forward
top level: BBa_K801063
start: 1
end: 456
orientation: inline

TEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2197861
display id: annotation2197861
version: 1
top level: BBa_K801063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197861/range2197861/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197861/range2197861/1
type: Range
persistent identity: range2197861
display id: range2197861
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2143
end: 2652
orientation: inline

BBa_K122000
type: ComponentDefinition
persistent identity: BBa K122000
display id: BBa_K122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pPGK1
creator: David Ouyang
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _218_
m user id: 0
owner id: 3326
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
mutable notes: Source: Saccharomyces cerevisiae genomic DNA.
mutable provenance: This part was obtained from yeast genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K122000_sequence/1

BBa_K792012
type: ComponentDefinition
persistent identity: BBa K792012
display id: BBa_K792012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast exportable Trp-rich peptide w/enhanced import (2)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pato
mutable notes: Composite device
mutable provenance: Composite device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K792004
component: BBa_K416003
component: BBa_K792008
component: BBa_J63003
component: BBa_K792013
sequence annotation: BBa_K792004
sequence annotation: BBa_K792013
sequence annotation: BBa_J63003
sequence annotation: BBa_K416003
sequence annotation: BBa_K792008
sequence: https://synbiohub.org/public/igem/BBa_K792012_sequence/1

BBa_K792004
type: Component
persistent identity: component2197294
display id: component2197294
version: 1
top level: BBa_K792012
access: public
definition: Polyargini

BBa_K416003
type: Component
persistent identity: component2197292
display id: component2197292
version: 1
top level: BBa_K792012
access: public
definition: BBa_K416003

BBa_K792008
type: Component
persistent identity: component2197297
display id: component2197297
version: 1
top level: BBa_K792012
access: public
definition: PolyWb

BBa_J63003
type: Component
persistent identity: component2197291
display id: component2197291
version: 1
top level: BBa_K792012
access: public
definition: Kozak & st

BBa_K792013
type: Component
persistent identity: component2197289
display id: component2197289
version: 1
top level: BBa_K792012
access: public
definition: https://synbiohub.org/public/igem/BBa_K792013/1

BBa_K792004
type: SequenceAnnotation
persistent identity: annotation2197294
display id: annotation2197294
version: 1
top level: BBa_K792012
location: https://synbiohub.org/public/igem/BBa_K792012/annotation2197294/range2197294/1
component: BBa_K792004

https://synbiohub.org/public/igem/BBa_K792012/annotation2197294/range2197294/1
type: Range
persistent identity: range2197294
display id: range2197294
version: 1
direction: direction/reverse
top level: BBa_K792012
start: 139
end: 177
orientation: inline

BBa_K792013
type: SequenceAnnotation
persistent identity: annotation2197289
display id: annotation2197289
version: 1
top level: BBa_K792012
location: https://synbiohub.org/public/igem/BBa_K792012/annotation2197289/range2197289/1
component: BBa_K792013

https://synbiohub.org/public/igem/BBa_K792012/annotation2197289/range2197289/1
type: Range
persistent identity: range2197289
display id: range2197289
version: 1
direction: direction/reverse
top level: BBa_K792012
start: 1
end: 6
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2197291
display id: annotation2197291
version: 1
top level: BBa_K792012
location: https://synbiohub.org/public/igem/BBa_K792012/annotation2197291/range2197291/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_K792012/annotation2197291/range2197291/1
type: Range
persistent identity: range2197291
display id: range2197291
version: 1
direction: direction/reverse
top level: BBa_K792012
start: 7
end: 24
orientation: inline

BBa_K416003
type: SequenceAnnotation
persistent identity: annotation2197292
display id: annotation2197292
version: 1
top level: BBa_K792012
location: https://synbiohub.org/public/igem/BBa_K792012/annotation2197292/range2197292/1
component: BBa_K416003

https://synbiohub.org/public/igem/BBa_K792012/annotation2197292/range2197292/1
type: Range
persistent identity: range2197292
display id: range2197292
version: 1
direction: direction/reverse
top level: BBa_K792012
start: 25
end: 138
orientation: inline

BBa_K792008
type: SequenceAnnotation
persistent identity: annotation2197297
display id: annotation2197297
version: 1
top level: BBa_K792012
location: https://synbiohub.org/public/igem/BBa_K792012/annotation2197297/range2197297/1
component: BBa_K792008

https://synbiohub.org/public/igem/BBa_K792012/annotation2197297/range2197297/1
type: Range
persistent identity: range2197297
display id: range2197297
version: 1
direction: direction/reverse
top level: BBa_K792012
start: 178
end: 297
orientation: inline

hsl1
type: ComponentDefinition
persistent identity: BBa K105015
display id: BBa_K105015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hsl1 - cell cycle dependent degradation tag in yeast
creator: Robin Sorg
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is a fragement of the yeast hsl1 protein. Fused to an artificial transcription factor it tiggers the degradation in??? phase via the ???.
mutable notes: To enable the fusion with the protein in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon. For more information about this issus, see: Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K105015_sequence/1

BBa_K165050
type: ComponentDefinition
persistent identity: BBa K165050
display id: BBa_K165050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full Gli1 Repressor (tagged with mCherryx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+Gli1 DBD+mCherryx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165004
component: BBa_K165002
component: BBa_K165003
component: BBa_K165018
component: BBa_K165007
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165004
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165050_sequence/1

BBa_K165004
type: Component
persistent identity: component1996712
display id: component1996712
version: 1
top level: BBa_K165050
access: public
definition: mCherry

BBa_K165002
type: Component
persistent identity: component1996708
display id: component1996708
version: 1
top level: BBa_K165050
access: public
definition: BBa_K165002

BBa_K165003
type: Component
persistent identity: component1996709
display id: component1996709
version: 1
top level: BBa_K165050
access: public
definition: Sin3

BBa_K165018
type: Component
persistent identity: component1996713
display id: component1996713
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165007
type: Component
persistent identity: component1996710
display id: component1996710
version: 1
top level: BBa_K165050
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165004
type: Component
persistent identity: component1996711
display id: component1996711
version: 1
top level: BBa_K165050
access: public
definition: mCherry

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996709
display id: annotation1996709
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996709/range1996709/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165050/annotation1996709/range1996709/1
type: Range
persistent identity: range1996709
display id: range1996709
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 27
end: 2837
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996713
display id: annotation1996713
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996713/range1996713/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165050/annotation1996713/range1996713/1
type: Range
persistent identity: range1996713
display id: range1996713
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 4865
end: 5117
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1996710
display id: annotation1996710
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996710/range1996710/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165050/annotation1996710/range1996710/1
type: Range
persistent identity: range1996710
display id: range1996710
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 2846
end: 3370
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996712
display id: annotation1996712
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996712/range1996712/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165050/annotation1996712/range1996712/1
type: Range
persistent identity: range1996712
display id: range1996712
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 4122
end: 4856
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996708
display id: annotation1996708
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996708/range1996708/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165050/annotation1996708/range1996708/1
type: Range
persistent identity: range1996708
display id: range1996708
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 1
end: 18
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996711
display id: annotation1996711
version: 1
top level: BBa_K165050
location: https://synbiohub.org/public/igem/BBa_K165050/annotation1996711/range1996711/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165050/annotation1996711/range1996711/1
type: Range
persistent identity: range1996711
display id: range1996711
version: 1
direction: direction/reverse
top level: BBa_K165050
start: 3379
end: 4113
orientation: inline

BBa_K211002
type: ComponentDefinition
persistent identity: BBa K211002
display id: BBa_K211002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-odr10 chimeric GPCR
creator: ZHONG Wanting
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _313_
m user id: 0
owner id: 4277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The chimeric GPCR consists of the N- and C- terminals of the rat OR RI7, flanking the TM2-TM7 ligand-binding domain of the c. elegans OR odr-10. It can be functionally expressed in S. cerevisiae, activating the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.
mutable provenance: C. elegans, Rattus norvegicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: diacetyl sensing region
sequence annotation: membrane localization sequence
sequence annotation: Gasubunit coupling sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K211002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2035762
display id: annotation2035762
version: 1
top level: BBa_K211002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1
type: Range
persistent identity: range2035762
display id: range2035762
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1057
end: 1062
orientation: inline

diacetyl sensing region
type: SequenceAnnotation
persistent identity: annotation2049260
display id: annotation2049260
version: 1
top level: BBa_K211002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1
type: Range
persistent identity: range2049260
display id: range2049260
version: 1
direction: direction/forward
top level: BBa_K211002
start: 184
end: 957
orientation: inline

membrane localization sequence
type: SequenceAnnotation
persistent identity: annotation2049258
display id: annotation2049258
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1
type: Range
persistent identity: range2049258
display id: range2049258
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 183
orientation: inline

Gasubunit coupling sequence
type: SequenceAnnotation
persistent identity: annotation2049259
display id: annotation2049259
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1
type: Range
persistent identity: range2049259
display id: range2049259
version: 1
direction: direction/forward
top level: BBa_K211002
start: 958
end: 1062
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2035761
display id: annotation2035761
version: 1
top level: BBa_K211002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1
type: Range
persistent identity: range2035761
display id: range2035761
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 3
orientation: inline

BBa_K165052
type: ComponentDefinition
persistent identity: BBa K165052
display id: BBa_K165052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full Zif268-HIV Repressor (tagged with mCherryx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+Zif268-HIV DBD+mCherryx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165004
component: BBa_K165002
component: BBa_K165003
component: BBa_K165006
component: BBa_K165018
sequence annotation: BBa_K165004
sequence annotation: BBa_K165003
sequence annotation: BBa_K165002
sequence annotation: BBa_K165006
sequence annotation: BBa_K165018
sequence: https://synbiohub.org/public/igem/BBa_K165052_sequence/1

BBa_K165004
type: Component
persistent identity: component1996724
display id: component1996724
version: 1
top level: BBa_K165052
access: public
definition: mCherry

BBa_K165002
type: Component
persistent identity: component1996721
display id: component1996721
version: 1
top level: BBa_K165052
access: public
definition: BBa_K165002

BBa_K165003
type: Component
persistent identity: component1996722
display id: component1996722
version: 1
top level: BBa_K165052
access: public
definition: Sin3

BBa_K165006
type: Component
persistent identity: component1996723
display id: component1996723
version: 1
top level: BBa_K165052
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165018
type: Component
persistent identity: component1996726
display id: component1996726
version: 1
top level: BBa_K165052
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1996725
display id: component1996725
version: 1
top level: BBa_K165052
access: public
definition: mCherry

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996725
display id: annotation1996725
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996725/range1996725/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165052/annotation1996725/range1996725/1
type: Range
persistent identity: range1996725
display id: range1996725
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 3849
end: 4583
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996722
display id: annotation1996722
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996722/range1996722/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165052/annotation1996722/range1996722/1
type: Range
persistent identity: range1996722
display id: range1996722
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 27
end: 2837
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996721
display id: annotation1996721
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996721/range1996721/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165052/annotation1996721/range1996721/1
type: Range
persistent identity: range1996721
display id: range1996721
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 1
end: 18
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1996723
display id: annotation1996723
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996723/range1996723/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165052/annotation1996723/range1996723/1
type: Range
persistent identity: range1996723
display id: range1996723
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 2846
end: 3097
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996726
display id: annotation1996726
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996726/range1996726/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165052/annotation1996726/range1996726/1
type: Range
persistent identity: range1996726
display id: range1996726
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 4592
end: 4844
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996724
display id: annotation1996724
version: 1
top level: BBa_K165052
location: https://synbiohub.org/public/igem/BBa_K165052/annotation1996724/range1996724/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165052/annotation1996724/range1996724/1
type: Range
persistent identity: range1996724
display id: range1996724
version: 1
direction: direction/reverse
top level: BBa_K165052
start: 3106
end: 3840
orientation: inline

BBa_K1462030
type: ComponentDefinition
persistent identity: BBa K1462030
display id: BBa_K1462030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hbd
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462030_sequence/1

BBa_K1680015
type: ComponentDefinition
persistent identity: BBa K1680015
display id: BBa_K1680015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS315 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.
mutable notes: contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR
sequence annotation: lacZalpha
sequence annotation: T3 promotor
sequence annotation: LEU2
sequence annotation: f1 origin
sequence annotation: lac promotor
sequence annotation: ampR promotor
sequence annotation: LEU2 promotor
sequence annotation: T7 promotor
sequence annotation: pUC origin
sequence annotation: RFC10
sequence annotation: CEN/ARS yeast centromere
sequence: https://synbiohub.org/public/igem/BBa_K1680015_sequence/1

ampR
type: SequenceAnnotation
persistent identity: annotation2466201
display id: annotation2466201
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1
type: Range
persistent identity: range2466201
display id: range2466201
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 4405
end: 5265
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466196
display id: annotation2466196
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1
type: Range
persistent identity: range2466196
display id: range2466196
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3186
end: 3248
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466197
display id: annotation2466197
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1
type: Range
persistent identity: range2466197
display id: range2466197
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3205
end: 3223
orientation: inline

LEU2
type: SequenceAnnotation
persistent identity: annotation2466188
display id: annotation2466188
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1
type: Range
persistent identity: range2466188
display id: range2466188
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 663
end: 1757
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466190
display id: annotation2466190
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1
type: Range
persistent identity: range2466190
display id: range2466190
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2474
end: 2929
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466199
display id: annotation2466199
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1
type: Range
persistent identity: range2466199
display id: range2466199
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3292
end: 3322
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466191
display id: annotation2466191
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1
type: Range
persistent identity: range2466191
display id: range2466191
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2712
end: 3128
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466202
display id: annotation2466202
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1
type: Range
persistent identity: range2466202
display id: range2466202
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 5266
end: 5370
orientation: inline

LEU2 promotor
type: SequenceAnnotation
persistent identity: annotation2466189
display id: annotation2466189
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1
type: Range
persistent identity: range2466189
display id: range2466189
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 1770
end: 2174
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466192
display id: annotation2466192
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1
type: Range
persistent identity: range2466192
display id: range2466192
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3097
end: 3115
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466200
display id: annotation2466200
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1
type: Range
persistent identity: range2466200
display id: range2466200
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3646
end: 4234
orientation: inline

RFC10
type: SequenceAnnotation
persistent identity: annotation2466193
display id: annotation2466193
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1
type: Range
persistent identity: range2466193
display id: range2466193
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3134
end: 3176
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466204
display id: annotation2466204
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1
type: Range
persistent identity: range2466204
display id: range2466204
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 5407
end: 5910
orientation: inline

TrpZipper2
type: ComponentDefinition
persistent identity: BBa K792006
display id: BBa_K792006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tryptophan rich peptide (TrpZipper2)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: '''TrpZipper''' is a small peptide that folds into a beta-hairpin secondary structure. The indole rings of the Trp form a hydrophobic core. The protein is water soluble and monomer.
mutable notes: Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Tryptophan zippers: Stable, monomeric beta-hairpins [Cochran 2001]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: stop
sequence annotation: HindIII restriction site
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K792006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2196616
display id: annotation2196616
version: 1
top level: TrpZipper2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2196616/range2196616/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2196616/range2196616/1
type: Range
persistent identity: range2196616
display id: range2196616
version: 1
direction: direction/forward
top level: TrpZipper2
start: 43
end: 48
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196458
display id: annotation2196458
version: 1
top level: TrpZipper2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2196458/range2196458/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2196458/range2196458/1
type: Range
persistent identity: range2196458
display id: range2196458
version: 1
direction: direction/forward
top level: TrpZipper2
start: 1
end: 6
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2197860
display id: annotation2197860
version: 1
top level: TrpZipper2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2197860/range2197860/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2197860/range2197860/1
type: Range
persistent identity: range2197860
display id: range2197860
version: 1
direction: direction/forward
top level: TrpZipper2
start: 7
end: 42
orientation: inline

BBa_K284000
type: ComponentDefinition
persistent identity: BBa K284000
display id: BBa_K284000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactate Permease from Kluyveromyces lactis
creator: Ra??ssa Estrela Curado, Ta??s Sineiro Herig and Gleidson Silva Teixeira
created: 2009-08-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: JEN1 from Kluyveromyces lactis is a homologue gene to JEN1 of Saccharomyces cerevisiae. It ecodes a monocarboxylate acids permease shared by lactic and pyruvic acids. Queiros O, Pereira L, Paiva S, Moradas-Ferreira P and Casal M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet 51, 161-169.
mutable notes: Working yet.
mutable provenance: This part was amplified by PCR from K. lactis genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: primer_binding
sequence annotation: Start
sequence annotation: Stop
sequence annotation: Lactate Permease
sequence: https://synbiohub.org/public/igem/BBa_K284000_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027259
display id: annotation2027259
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027259/range2027259/1
type: Range
persistent identity: range2027259
display id: range2027259
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1
end: 21
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2027261
display id: annotation2027261
version: 1
top level: BBa_K284000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027261/range2027261/1
type: Range
persistent identity: range2027261
display id: range2027261
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 9
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2027263
display id: annotation2027263
version: 1
top level: BBa_K284000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027263/range2027263/1
type: Range
persistent identity: range2027263
display id: range2027263
version: 1
direction: direction/reverse
top level: BBa_K284000
start: 1855
end: 1873
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2027262
display id: annotation2027262
version: 1
top level: BBa_K284000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027262/range2027262/1
type: Range
persistent identity: range2027262
display id: range2027262
version: 1
direction: direction/forward
top level: BBa_K284000
start: 1807
end: 1809
orientation: inline

Lactate Permease
type: SequenceAnnotation
persistent identity: annotation2027260
display id: annotation2027260
version: 1
top level: BBa_K284000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1

https://synbiohub.org/public/igem/BBa_K284000/annotation2027260/range2027260/1
type: Range
persistent identity: range2027260
display id: range2027260
version: 1
direction: direction/forward
top level: BBa_K284000
start: 7
end: 1809
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 yeast constitutive promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
mutable notes: -
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

Regional
type: ComponentDefinition
persistent identity: BBa K106676
display id: BBa_K106676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regional silencing construct
creator: Jacinto Chen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3418
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cyc1p-mCherry-Adh1t-8xLexA Operators-Cyc1p-GFP-Adh1t
mutable notes: Subcloned and Aar1 assembly.
mutable provenance: none.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K106674
component: BBa_K106675
sequence annotation: BBa_K106674
sequence annotation: BBa_K106675
sequence: https://synbiohub.org/public/igem/BBa_K106676_sequence/1

BBa_K106674
type: Component
persistent identity: component2224182
display id: component2224182
version: 1
top level: Regional
access: public
definition: https://synbiohub.org/public/igem/BBa_K106674/1

BBa_K106675
type: Component
persistent identity: component2224174
display id: component2224174
version: 1
top level: Regional
access: public
definition: https://synbiohub.org/public/igem/BBa_K106675/1

BBa_K106674
type: SequenceAnnotation
persistent identity: annotation2224182
display id: annotation2224182
version: 1
top level: Regional
location: https://synbiohub.org/public/igem/BBa_K106676/annotation2224182/range2224182/1
component: BBa_K106674

https://synbiohub.org/public/igem/BBa_K106676/annotation2224182/range2224182/1
type: Range
persistent identity: range2224182
display id: range2224182
version: 1
direction: direction/reverse
top level: Regional
start: 1171
end: 2526
orientation: inline

BBa_K106675
type: SequenceAnnotation
persistent identity: annotation2224174
display id: annotation2224174
version: 1
top level: Regional
location: https://synbiohub.org/public/igem/BBa_K106676/annotation2224174/range2224174/1
component: BBa_K106675

https://synbiohub.org/public/igem/BBa_K106676/annotation2224174/range2224174/1
type: Range
persistent identity: range2224174
display id: range2224174
version: 1
direction: direction/reverse
top level: Regional
start: 1
end: 1170
orientation: inline

MFa1-Kozak
type: ComponentDefinition
persistent identity: BBa K792001
display id: BBa_K792001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak sequence from yeast α-factor mating pheromone (MFα1)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Kozak sequence is the eukaryotic analog of the bacterial RBS, it is short sequence that includes the ATG, required for proficient initiation of translation. It is known that the 5???UTR sequence of the MFα1 gene of yeast produces efficient initiation of translation. This part is that specific region of the MFα1 gene.
mutable notes: None
mutable provenance: http://www.yeastgenome.org/cgi-bin/locus.fpl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BamHI restriction site
sequence annotation: rbs
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K792001_sequence/1

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2197870
display id: annotation2197870
version: 1
top level: MFa1-Kozak
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792001/annotation2197870/range2197870/1

https://synbiohub.org/public/igem/BBa_K792001/annotation2197870/range2197870/1
type: Range
persistent identity: range2197870
display id: range2197870
version: 1
direction: direction/forward
top level: MFa1-Kozak
start: 1
end: 6
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2197871
display id: annotation2197871
version: 1
top level: MFa1-Kozak
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K792001/annotation2197871/range2197871/1

https://synbiohub.org/public/igem/BBa_K792001/annotation2197871/range2197871/1
type: Range
persistent identity: range2197871
display id: range2197871
version: 1
direction: direction/forward
top level: MFa1-Kozak
start: 7
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2197872
display id: annotation2197872
version: 1
top level: MFa1-Kozak
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K792001/annotation2197872/range2197872/1

https://synbiohub.org/public/igem/BBa_K792001/annotation2197872/range2197872/1
type: Range
persistent identity: range2197872
display id: range2197872
version: 1
direction: direction/forward
top level: MFa1-Kozak
start: 19
end: 21
orientation: inline

SV40 NLS
type: ComponentDefinition
persistent identity: BBa J63008
display id: BBa_J63008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 nuclear localization sequence from SV40; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized nuclear localization sequence from simian virus 40
mutable notes: as described in literature
mutable provenance: direct synthesis from published sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63008_sequence/1

BBa_K801038
type: ComponentDefinition
persistent identity: BBa K801038
display id: BBa_K801038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhyB-linker-Gal4bd
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
mutable notes: -
mutable provenance: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801037
sequence annotation: RFC25 fusion scar
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: BBa_K801032
sequence annotation: BBa_K801031
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801038_sequence/1

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2198916
display id: annotation2198916
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1
type: Range
persistent identity: range2198916
display id: range2198916
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1066
end: 1068
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2198921
display id: annotation2198921
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1
type: Range
persistent identity: range2198921
display id: range2198921
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 3231
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198923
display id: annotation2198923
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1
type: Range
persistent identity: range2198923
display id: range2198923
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2722
end: 2727
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198919
display id: annotation2198919
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1
type: Range
persistent identity: range2198919
display id: range2198919
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2788
end: 2793
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198912
display id: annotation2198912
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1
type: Range
persistent identity: range2198912
display id: range2198912
version: 1
direction: direction/forward
top level: BBa_K801038
start: 325
end: 327
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198914
display id: annotation2198914
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1
type: Range
persistent identity: range2198914
display id: range2198914
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1039
end: 1041
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2198920
display id: annotation2198920
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1
type: Range
persistent identity: range2198920
display id: range2198920
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2794
end: 3231
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198913
display id: annotation2198913
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1
type: Range
persistent identity: range2198913
display id: range2198913
version: 1
direction: direction/forward
top level: BBa_K801038
start: 328
end: 330
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2198917
display id: annotation2198917
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1
type: Range
persistent identity: range2198917
display id: range2198917
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1
end: 2721
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198915
display id: annotation2198915
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1
type: Range
persistent identity: range2198915
display id: range2198915
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1051
end: 1053
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198918
display id: annotation2198918
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1
type: Range
persistent identity: range2198918
display id: range2198918
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 2787
orientation: inline

BBa_K792010
type: ComponentDefinition
persistent identity: BBa K792010
display id: BBa_K792010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast exportable Trp-rich peptide w/enhanced import (1)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite device produces and '''exports''' a '''Histidine rich''' peptide, that is '''import enhanced''' (thanks to a trojan peptide). This device is intended to be used with '''Yeast chassis''', so it is composed by subparts designed also to be use in yeast. The high level structure of the device is: * '''''Kozak''''' consensus sequence for initiation of translation ''(from yeast α-factor mating pheromone (MFα1))'' * '''''Signal''''' peptide that targets the product of the gene for secretion ''(also from yeast α-factor mating pheromone (MFα1))'' * '''''Trojan''''' peptide, to increase internalization in target cell ''(from HIV TAT penetratin)'' * '''''Payload''''': this is the exported '''Histidine''' rich domain of the protein ''(a His-Tag)'' The device's sequence has inherited some useful '''restriction sites''' (''BamHI'' & ''HindIII'') from the parts it's made of. '''Important note''': this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from ''BB assembly standard''.
mutable notes: Composite device
mutable provenance: Composite device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K792006
component: BBa_K792002
component: BBa_K792001
component: BBa_K792003
sequence annotation: BBa_K792001
sequence annotation: BBa_K792003
sequence annotation: BBa_K792006
sequence annotation: BBa_K792002
sequence: https://synbiohub.org/public/igem/BBa_K792010_sequence/1

BBa_K792006
type: Component
persistent identity: component2196549
display id: component2196549
version: 1
top level: BBa_K792010
access: public
definition: TrpZipper2

BBa_K792002
type: Component
persistent identity: component2196545
display id: component2196545
version: 1
top level: BBa_K792010
access: public
definition: MFa1-Secre

BBa_K792001
type: Component
persistent identity: component2196544
display id: component2196544
version: 1
top level: BBa_K792010
access: public
definition: MFa1-Kozak

BBa_K792003
type: Component
persistent identity: component2196547
display id: component2196547
version: 1
top level: BBa_K792010
access: public
definition: TAT-trojan

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2196544
display id: annotation2196544
version: 1
top level: BBa_K792010
location: https://synbiohub.org/public/igem/BBa_K792010/annotation2196544/range2196544/1
component: BBa_K792001

https://synbiohub.org/public/igem/BBa_K792010/annotation2196544/range2196544/1
type: Range
persistent identity: range2196544
display id: range2196544
version: 1
direction: direction/reverse
top level: BBa_K792010
start: 1
end: 21
orientation: inline

BBa_K792003
type: SequenceAnnotation
persistent identity: annotation2196547
display id: annotation2196547
version: 1
top level: BBa_K792010
location: https://synbiohub.org/public/igem/BBa_K792010/annotation2196547/range2196547/1
component: BBa_K792003

https://synbiohub.org/public/igem/BBa_K792010/annotation2196547/range2196547/1
type: Range
persistent identity: range2196547
display id: range2196547
version: 1
direction: direction/reverse
top level: BBa_K792010
start: 76
end: 114
orientation: inline

BBa_K792006
type: SequenceAnnotation
persistent identity: annotation2196549
display id: annotation2196549
version: 1
top level: BBa_K792010
location: https://synbiohub.org/public/igem/BBa_K792010/annotation2196549/range2196549/1
component: BBa_K792006

https://synbiohub.org/public/igem/BBa_K792010/annotation2196549/range2196549/1
type: Range
persistent identity: range2196549
display id: range2196549
version: 1
direction: direction/reverse
top level: BBa_K792010
start: 115
end: 162
orientation: inline

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2196545
display id: annotation2196545
version: 1
top level: BBa_K792010
location: https://synbiohub.org/public/igem/BBa_K792010/annotation2196545/range2196545/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K792010/annotation2196545/range2196545/1
type: Range
persistent identity: range2196545
display id: range2196545
version: 1
direction: direction/reverse
top level: BBa_K792010
start: 22
end: 75
orientation: inline

BBa_K319041
type: ComponentDefinition
persistent identity: BBa K319041
display id: BBa_K319041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NatMX cassette
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the cassette (promoter+coding region+terminator) that imparts resistance to nourseothricin in yeast.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: NatMX ORF
sequence annotation: TEF1 promoter
sequence annotation: TEF1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K319041_sequence/1

NatMX ORF
type: SequenceAnnotation
persistent identity: annotation2109282
display id: annotation2109282
version: 1
top level: BBa_K319041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1
type: Range
persistent identity: range2109282
display id: range2109282
version: 1
direction: direction/forward
top level: BBa_K319041
start: 380
end: 955
orientation: inline

TEF1 promoter
type: SequenceAnnotation
persistent identity: annotation2109281
display id: annotation2109281
version: 1
top level: BBa_K319041
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1
type: Range
persistent identity: range2109281
display id: range2109281
version: 1
direction: direction/forward
top level: BBa_K319041
start: 1
end: 379
orientation: inline

TEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2109283
display id: annotation2109283
version: 1
top level: BBa_K319041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1
type: Range
persistent identity: range2109283
display id: range2109283
version: 1
direction: direction/forward
top level: BBa_K319041
start: 956
end: 1190
orientation: inline

BBa_K110005
type: ComponentDefinition
persistent identity: BBa K110005
display id: BBa_K110005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)2
sequence: https://synbiohub.org/public/igem/BBa_K110005_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997684
display id: annotation1997684
version: 1
top level: BBa_K110005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1
type: Range
persistent identity: range1997684
display id: range1997684
version: 1
direction: direction/forward
top level: BBa_K110005
start: 184
end: 209
orientation: inline

MF(alpha)2
type: SequenceAnnotation
persistent identity: annotation1997686
display id: annotation1997686
version: 1
top level: BBa_K110005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1
type: Range
persistent identity: range1997686
display id: range1997686
version: 1
direction: direction/forward
top level: BBa_K110005
start: 1
end: 500
orientation: inline

BBa_K1486035
type: ComponentDefinition
persistent identity: BBa K1486035
display id: BBa_K1486035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486025
component: BBa_K1486031
component: BBa_K1486034
component: BBa_K1486037
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486034
sequence annotation: BBa_K1486025
sequence: https://synbiohub.org/public/igem/BBa_K1486035_sequence/1

BBa_K1486025
type: Component
persistent identity: component2418331
display id: component2418331
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486025

BBa_K1486031
type: Component
persistent identity: component2418335
display id: component2418335
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486031

BBa_K1486034
type: Component
persistent identity: component2418330
display id: component2418330
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486034

BBa_K1486037
type: Component
persistent identity: component2418328
display id: component2418328
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418335
display id: annotation2418335
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
type: Range
persistent identity: range2418335
display id: range2418335
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 303
end: 1662
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418328
display id: annotation2418328
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
type: Range
persistent identity: range2418328
display id: range2418328
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 1
end: 39
orientation: inline

BBa_K1486034
type: SequenceAnnotation
persistent identity: annotation2418330
display id: annotation2418330
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
component: BBa_K1486034

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
type: Range
persistent identity: range2418330
display id: range2418330
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 40
end: 114
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418331
display id: annotation2418331
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
type: Range
persistent identity: range2418331
display id: range2418331
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 115
end: 302
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Help:Barcodes
sequence annotation: mCherry
sequence annotation: GSGTA linker
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
direction: direction/forward
top level: mCherry
start: 745
end: 769
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 738
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
direction: direction/forward
top level: mCherry
start: 724
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 15
orientation: inline

BBa_K801073
type: ComponentDefinition
persistent identity: BBa K801073
display id: BBa_K801073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
mutable notes: -
mutable provenance: Composite part of BBa_K801010, BBa_K801070, BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: ADH1 Terminator
sequence annotation: Stop codon
sequence annotation: TEF2 promoter
sequence annotation: Strep-Tag
sequence annotation: CaXMT1
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K801073_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199478
display id: annotation2199478
version: 1
top level: BBa_K801073
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199478/range2199478/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199478/range2199478/1
type: Range
persistent identity: range2199478
display id: range2199478
version: 1
direction: direction/forward
top level: BBa_K801073
start: 657
end: 662
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201181
display id: annotation2201181
version: 1
top level: BBa_K801073
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2201181/range2201181/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2201181/range2201181/1
type: Range
persistent identity: range2201181
display id: range2201181
version: 1
direction: direction/forward
top level: BBa_K801073
start: 663
end: 665
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197265
display id: annotation2197265
version: 1
top level: BBa_K801073
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197265/range2197265/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197265/range2197265/1
type: Range
persistent identity: range2197265
display id: range2197265
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1823
end: 2172
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199450
display id: annotation2199450
version: 1
top level: BBa_K801073
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199450/range2199450/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199450/range2199450/1
type: Range
persistent identity: range2199450
display id: range2199450
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1809
end: 1814
orientation: inline

TEF2 promoter
type: SequenceAnnotation
persistent identity: annotation2197263
display id: annotation2197263
version: 1
top level: BBa_K801073
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197263/range2197263/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197263/range2197263/1
type: Range
persistent identity: range2197263
display id: range2197263
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1
end: 648
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199452
display id: annotation2199452
version: 1
top level: BBa_K801073
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199452/range2199452/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199452/range2199452/1
type: Range
persistent identity: range2199452
display id: range2199452
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1779
end: 1808
orientation: inline

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2197264
display id: annotation2197264
version: 1
top level: BBa_K801073
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197264/range2197264/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197264/range2197264/1
type: Range
persistent identity: range2197264
display id: range2197264
version: 1
direction: direction/forward
top level: BBa_K801073
start: 663
end: 1778
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation2197262
display id: annotation2197262
version: 1
top level: BBa_K801073
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197262/range2197262/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197262/range2197262/1
type: Range
persistent identity: range2197262
display id: range2197262
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1
end: 2172
orientation: inline

BBa_K801080
type: ComponentDefinition
persistent identity: BBa K801080
display id: BBa_K801080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prepro-Thaumatin, yeast codon optimized
creator: Alois Br??uer
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Coding region for Prepro-Thaumatin. Codon optimized for yeast.
mutable notes: Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.
mutable provenance: Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Pre-signal sequence
sequence annotation: Pro-signal sequence
sequence annotation: Preprothaumatin
sequence: https://synbiohub.org/public/igem/BBa_K801080_sequence/1

Pre-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343094
display id: annotation2343094
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1
type: Range
persistent identity: range2343094
display id: range2343094
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 67
orientation: inline

Pro-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343095
display id: annotation2343095
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1
type: Range
persistent identity: range2343095
display id: range2343095
version: 1
direction: direction/forward
top level: BBa_K801080
start: 688
end: 705
orientation: inline

Preprothaumatin
type: SequenceAnnotation
persistent identity: annotation2343093
display id: annotation2343093
version: 1
top level: BBa_K801080
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1
type: Range
persistent identity: range2343093
display id: range2343093
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 705
orientation: inline

BBa_K863204
type: ComponentDefinition
persistent identity: BBa K863204
display id: BBa_K863204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: shuttle vector pECPP11JS for recombination of genes of interest in yeast
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: long description is coming soon
mutable notes: the design considerations are coming soon
mutable provenance: This part is made of the BioBricks XYZ by gibson assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: 5' AOX
sequence annotation: Kosak sequence
sequence annotation: MFalpha
sequence annotation: 3' AOX
sequence annotation: AOX1 terminator
sequence annotation: his4
sequence: https://synbiohub.org/public/igem/BBa_K863204_sequence/1

5' AOX
type: SequenceAnnotation
persistent identity: annotation2200002
display id: annotation2200002
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200002/range2200002/1
type: Range
persistent identity: range2200002
display id: range2200002
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1
end: 940
orientation: inline

Kosak sequence
type: SequenceAnnotation
persistent identity: annotation2200003
display id: annotation2200003
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200003/range2200003/1
type: Range
persistent identity: range2200003
display id: range2200003
version: 1
direction: direction/forward
top level: BBa_K863204
start: 941
end: 952
orientation: inline

MFalpha
type: SequenceAnnotation
persistent identity: annotation2200004
display id: annotation2200004
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200004/range2200004/1
type: Range
persistent identity: range2200004
display id: range2200004
version: 1
direction: direction/forward
top level: BBa_K863204
start: 953
end: 1215
orientation: inline

3' AOX
type: SequenceAnnotation
persistent identity: annotation2200007
display id: annotation2200007
version: 1
top level: BBa_K863204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200007/range2200007/1
type: Range
persistent identity: range2200007
display id: range2200007
version: 1
direction: direction/forward
top level: BBa_K863204
start: 4184
end: 4867
orientation: inline

AOX1 terminator
type: SequenceAnnotation
persistent identity: annotation2200005
display id: annotation2200005
version: 1
top level: BBa_K863204
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200005/range2200005/1
type: Range
persistent identity: range2200005
display id: range2200005
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1250
end: 1531
orientation: inline

his4
type: SequenceAnnotation
persistent identity: annotation2200006
display id: annotation2200006
version: 1
top level: BBa_K863204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1

https://synbiohub.org/public/igem/BBa_K863204/annotation2200006/range2200006/1
type: Range
persistent identity: range2200006
display id: range2200006
version: 1
direction: direction/forward
top level: BBa_K863204
start: 1615
end: 4143
orientation: inline

BBa_K801032
type: ComponentDefinition
persistent identity: BBa K801032
display id: BBa_K801032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 DNA binding domain
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Gal4 binding domain
mutable notes: -
mutable provenance: extracted from "pGBKT7" from clontech, with RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K801032_sequence/1

BBa_K801042
type: ComponentDefinition
persistent identity: BBa K801042
display id: BBa_K801042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 based yeast light-switchable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 promoter (for sequencing)
sequence annotation: GAL4DBD (GAL4 DNA binding domain)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: Linker
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
sequence annotation: HA epitope tage
sequence annotation: SV40NLS-PhyB-Linker-GAL4DBD
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: 20 amino acids long linker (BBa_K365005)
sequence: https://synbiohub.org/public/igem/BBa_K801042_sequence/1

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198786
display id: annotation2198786
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1
type: Range
persistent identity: range2198786
display id: range2198786
version: 1
direction: direction/forward
top level: BBa_K801042
start: 869
end: 891
orientation: inline

GAL4DBD (GAL4 DNA binding domain)
type: SequenceAnnotation
persistent identity: annotation2198796
display id: annotation2198796
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1
type: Range
persistent identity: range2198796
display id: range2198796
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5102
end: 5539
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198816
display id: annotation2198816
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1
type: Range
persistent identity: range2198816
display id: range2198816
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5557
end: 6063
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198794
display id: annotation2198794
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1
type: Range
persistent identity: range2198794
display id: range2198794
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2309
end: 5029
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198790
display id: annotation2198790
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1
type: Range
persistent identity: range2198790
display id: range2198790
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1782
end: 2231
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198784
display id: annotation2198784
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1
type: Range
persistent identity: range2198784
display id: range2198784
version: 1
direction: direction/forward
top level: BBa_K801042
start: 525
end: 863
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198785
display id: annotation2198785
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1
type: Range
persistent identity: range2198785
display id: range2198785
version: 1
direction: direction/forward
top level: BBa_K801042
start: 864
end: 959
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198781
display id: annotation2198781
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1
type: Range
persistent identity: range2198781
display id: range2198781
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1
end: 450
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198788
display id: annotation2198788
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1
type: Range
persistent identity: range2198788
display id: range2198788
version: 1
direction: direction/forward
top level: BBa_K801042
start: 960
end: 1256
orientation: inline

BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
type: SequenceAnnotation
persistent identity: annotation2198793
display id: annotation2198793
version: 1
top level: BBa_K801042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1
type: Range
persistent identity: range2198793
display id: range2198793
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 5539
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198787
display id: annotation2198787
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1
type: Range
persistent identity: range2198787
display id: range2198787
version: 1
direction: direction/forward
top level: BBa_K801042
start: 912
end: 932
orientation: inline

SV40NLS-PhyB-Linker-GAL4DBD
type: SequenceAnnotation
persistent identity: annotation2198791
display id: annotation2198791
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1
type: Range
persistent identity: range2198791
display id: range2198791
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2237
end: 5548
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198792
display id: annotation2198792
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1
type: Range
persistent identity: range2198792
display id: range2198792
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 2302
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198782
display id: annotation2198782
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1
type: Range
persistent identity: range2198782
display id: range2198782
version: 1
direction: direction/forward
top level: BBa_K801042
start: 456
end: 1259
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198789
display id: annotation2198789
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1
type: Range
persistent identity: range2198789
display id: range2198789
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1268
end: 1774
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198783
display id: annotation2198783
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1
type: Range
persistent identity: range2198783
display id: range2198783
version: 1
direction: direction/forward
top level: BBa_K801042
start: 465
end: 521
orientation: inline

20 amino acids long linker (BBa_K365005)
type: SequenceAnnotation
persistent identity: annotation2198795
display id: annotation2198795
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1
type: Range
persistent identity: range2198795
display id: range2198795
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5036
end: 5095
orientation: inline

BBa_K1680008
type: ComponentDefinition
persistent identity: BBa K1680008
display id: BBa_K1680008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Δ1-19 Cre recombinase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:34:31Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a mutant (Deletion of AA1-19) of DNA recombinase Cre, codon optimized for yeast.
mutable notes: To be used with RFC25
mutable provenance: gBlock, designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Delta1-19 Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680008_sequence/1

Delta1-19 Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477412
display id: annotation2477412
version: 1
top level: BBa_K1680008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680008/annotation2477412/range2477412/1

https://synbiohub.org/public/igem/BBa_K1680008/annotation2477412/range2477412/1
type: Range
persistent identity: range2477412
display id: range2477412
version: 1
direction: direction/forward
top level: BBa_K1680008
start: 1
end: 972
orientation: inline

BBa_K801004
type: ComponentDefinition
persistent identity: BBa K801004
display id: BBa_K801004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM104 yeast shuttle vector with GAL1 promoter
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 ?? origin
sequence annotation: pUC1 origin
sequence annotation: CYC1 transcription terminator
sequence annotation: Ampicillin
sequence annotation: T7 promoter/priming site
sequence annotation: URA3
sequence annotation: f1 origin
sequence annotation: PGAL1
sequence: https://synbiohub.org/public/igem/BBa_K801004_sequence/1

2 ?? origin
type: SequenceAnnotation
persistent identity: annotation2213074
display id: annotation2213074
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1
type: Range
persistent identity: range2213074
display id: range2213074
version: 1
direction: direction/forward
top level: BBa_K801004
start: 3281
end: 4752
orientation: inline

pUC1 origin
type: SequenceAnnotation
persistent identity: annotation2213071
display id: annotation2213071
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1
type: Range
persistent identity: range2213071
display id: range2213071
version: 1
direction: direction/forward
top level: BBa_K801004
start: 474
end: 1147
orientation: inline

CYC1 transcription terminator
type: SequenceAnnotation
persistent identity: annotation2213070
display id: annotation2213070
version: 1
top level: BBa_K801004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1
type: Range
persistent identity: range2213070
display id: range2213070
version: 1
direction: direction/forward
top level: BBa_K801004
start: 44
end: 292
orientation: inline

Ampicillin
type: SequenceAnnotation
persistent identity: annotation2213072
display id: annotation2213072
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1
type: Range
persistent identity: range2213072
display id: range2213072
version: 1
direction: direction/forward
top level: BBa_K801004
start: 1292
end: 2152
orientation: inline

T7 promoter/priming site
type: SequenceAnnotation
persistent identity: annotation2213077
display id: annotation2213077
version: 1
top level: BBa_K801004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1
type: Range
persistent identity: range2213077
display id: range2213077
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5767
end: 5786
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2213073
display id: annotation2213073
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1
type: Range
persistent identity: range2213073
display id: range2213073
version: 1
direction: direction/forward
top level: BBa_K801004
start: 2170
end: 3277
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2213075
display id: annotation2213075
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1
type: Range
persistent identity: range2213075
display id: range2213075
version: 1
direction: direction/forward
top level: BBa_K801004
start: 4820
end: 5275
orientation: inline

PGAL1
type: SequenceAnnotation
persistent identity: annotation2213076
display id: annotation2213076
version: 1
top level: BBa_K801004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1
type: Range
persistent identity: range2213076
display id: range2213076
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5293
end: 5743
orientation: inline

BBa_K801060
type: ComponentDefinition
persistent identity: BBa K801060
display id: BBa_K801060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains the (+)-Limonene Synthase 1 of ''Citrus limon''. It is preceeded by the yeast consensus sequence for improved expression and carries a C-Terminal Strep-Tag for purification or detection by westernblot. It is an improved version of BBa_I742111.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/21435702
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Strep tag
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801060_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197169
display id: annotation2197169
version: 1
top level: BBa_K801060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197169/range2197169/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197169/range2197169/1
type: Range
persistent identity: range2197169
display id: range2197169
version: 1
direction: direction/forward
top level: BBa_K801060
start: 1
end: 7
orientation: inline

Strep tag
type: SequenceAnnotation
persistent identity: annotation2197211
display id: annotation2197211
version: 1
top level: BBa_K801060
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197211/range2197211/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197211/range2197211/1
type: Range
persistent identity: range2197211
display id: range2197211
version: 1
direction: direction/forward
top level: BBa_K801060
start: 1682
end: 1705
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197213
display id: annotation2197213
version: 1
top level: BBa_K801060
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197213/range2197213/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197213/range2197213/1
type: Range
persistent identity: range2197213
display id: range2197213
version: 1
direction: direction/forward
top level: BBa_K801060
start: 8
end: 1672
orientation: inline

BBa_K106697
type: ComponentDefinition
persistent identity: BBa K106697
display id: BBa_K106697
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t), with the addition of 8X LexA operator sites directly downstream of the Adht1 terminator.
mutable notes: None.
mutable provenance: Variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: 8X LexA Operators
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: lac promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: LEU2 marker
sequence annotation: CEN ARS
sequence annotation: T3 promoter
sequence annotation: f1 origin
sequence annotation: Start codon
sequence annotation: Adh1 terminator
sequence annotation: lacZalpha reporter
sequence annotation: ampicillin resistance promoter
sequence annotation: Cyc1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K106697_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999737
display id: annotation1999737
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1
type: Range
persistent identity: range1999737
display id: range1999737
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6556
end: 6559
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995079
display id: annotation1995079
version: 1
top level: BBa_K106697
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1
type: Range
persistent identity: range1995079
display id: range1995079
version: 1
direction: direction/forward
top level: BBa_K106697
start: 210
end: 377
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995018
display id: annotation1995018
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1
type: Range
persistent identity: range1995018
display id: range1995018
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4215
end: 5075
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999732
display id: annotation1999732
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1
type: Range
persistent identity: range1999732
display id: range1999732
version: 1
direction: direction/forward
top level: BBa_K106697
start: 5230
end: 5849
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999733
display id: annotation1999733
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1
type: Range
persistent identity: range1999733
display id: range1999733
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6158
end: 6187
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999735
display id: annotation1999735
version: 1
top level: BBa_K106697
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1
type: Range
persistent identity: range1999735
display id: range1999735
version: 1
direction: direction/forward
top level: BBa_K106697
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999728
display id: annotation1999728
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1
type: Range
persistent identity: range1999728
display id: range1999728
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 386
end: 404
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1995020
display id: annotation1995020
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1
type: Range
persistent identity: range1995020
display id: range1995020
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1
end: 4
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995101
display id: annotation1995101
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1
type: Range
persistent identity: range1995101
display id: range1995101
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1732
end: 2838
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995017
display id: annotation1995017
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1
type: Range
persistent identity: range1995017
display id: range1995017
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 3565
end: 4083
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999734
display id: annotation1999734
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1
type: Range
persistent identity: range1999734
display id: range1999734
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6257
end: 6276
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999730
display id: annotation1999730
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1
type: Range
persistent identity: range1999730
display id: range1999730
version: 1
direction: direction/forward
top level: BBa_K106697
start: 589
end: 895
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999736
display id: annotation1999736
version: 1
top level: BBa_K106697
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1
type: Range
persistent identity: range1999736
display id: range1999736
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6547
end: 6549
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995021
display id: annotation1995021
version: 1
top level: BBa_K106697
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1
type: Range
persistent identity: range1995021
display id: range1995021
version: 1
direction: direction/forward
top level: BBa_K106697
start: 30
end: 209
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999729
display id: annotation1999729
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1
type: Range
persistent identity: range1999729
display id: range1999729
version: 1
direction: direction/forward
top level: BBa_K106697
start: 408
end: 567
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999731
display id: annotation1999731
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1
type: Range
persistent identity: range1999731
display id: range1999731
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4145
end: 4173
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995019
display id: annotation1995019
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1
type: Range
persistent identity: range1995019
display id: range1995019
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6294
end: 6556
orientation: inline

BBa_K392003
type: ComponentDefinition
persistent identity: BBa K392003
display id: BBa_K392003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 terminator
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Engineered 'cyc100 promoter' of Saccharomyces cerevisiae with Kozak sequence attached downstream.
mutable notes: Assembled by 3A method.
mutable provenance: Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K392003_sequence/1

BBa_Y00029
type: ComponentDefinition
persistent identity: BBa Y00029
display id: BBa_Y00029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. pombe homolog of S.cerevisiae SGF29, recoded for expression in S.c.
creator: Natalie Kuldell
created: 2006-07-31T11:00:00Z
modified: 2015-05-08T01:14:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _43_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Y00029 is S. pombe gene SPBC1921.07c [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=protein&cmd=search&term=NP_596000.2] recoded for expression in S. cerevisiae. The pombe gene is the homolog of the cerevisiae SGF29 [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29], a subunit of the ATP-independent chromatin remodelling complex SAGA. Deletion of this gene in S. cerevisiae results in a viable strain. Requirements for SGF29 in SAGA function are largely unexplored. Unlike many of the SAGA subunits, the pombe and the cerevisiae gene encode proteins of abour the same size (259 aa in cerevisiae and 244 in pombe). The gene has been recoded for expression in S. cerevisiae by DNA 2.0, although it's not clear that this recoding will be necessary for expresssion. The recoded gene is 78% identical to the original pombe gene (Identities = 568/723).
mutable notes: insured no biobrick sites in ORF during optimization
mutable provenance: DNA 2.0 optimization of pombe genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: suffix
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_Y00029_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation1892459
display id: annotation1892459
version: 1
top level: BBa_Y00029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_Y00029/annotation1892459/range1892459/1

https://synbiohub.org/public/igem/BBa_Y00029/annotation1892459/range1892459/1
type: Range
persistent identity: range1892459
display id: range1892459
version: 1
direction: direction/forward
top level: BBa_Y00029
start: 766
end: 794
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1892458
display id: annotation1892458
version: 1
top level: BBa_Y00029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_Y00029/annotation1892458/range1892458/1

https://synbiohub.org/public/igem/BBa_Y00029/annotation1892458/range1892458/1
type: Range
persistent identity: range1892458
display id: range1892458
version: 1
direction: direction/forward
top level: BBa_Y00029
start: 1
end: 30
orientation: inline

BBa_K1680011
type: ComponentDefinition
persistent identity: BBa K1680011
display id: BBa_K1680011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HCV protease with linkers
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:38:42Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the Hepatitis C virus (HCV) NS3-4A protease with N- & C-terminal linkers. Intended for combination with 145N Dronpa (Part BBa_K1680006) as described by Zhou et al. 2012. Codon optimized for Yeast.
mutable notes: Shipped in RFC25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1680011_sequence/1

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directly from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: STE2 Promoter
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
direction: direction/forward
top level: BBa_K110016
start: 304
end: 311
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
direction: direction/forward
top level: BBa_K110016
start: 273
end: 282
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
direction: direction/forward
top level: BBa_K110016
start: 1
end: 500
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
direction: direction/forward
top level: BBa_K110016
start: 295
end: 302
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
direction: direction/forward
top level: BBa_K110016
start: 284
end: 293
orientation: inline

BBa_K1680006
type: ComponentDefinition
persistent identity: BBa K1680006
display id: BBa_K1680006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluorescent protein dronpa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 4206
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa, codon optimized for Yeast. The mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence: https://synbiohub.org/public/igem/BBa_K1680006_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2475196
display id: annotation2475196
version: 1
top level: BBa_K1680006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1

https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1
type: Range
persistent identity: range2475196
display id: range2475196
version: 1
direction: direction/forward
top level: BBa_K1680006
start: 1
end: 669
orientation: inline

ABF1
type: ComponentDefinition
persistent identity: BBa K976003
display id: BBa_K976003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Autonomously Replicating Sequence Binding Factor 1
creator: Amna Sohail
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Autonomously Replicating Sequence Binding Factor 1, or ABF1 is a site specific DNA-binding protein that can function in both transcriptional activation and repression in Saccharomyces cerevisiae. In particular, the Alcohol Dehydrogenase 1 (ADH1) promoter gene contains two ABF1 binding sites in its promoter and coding regions. Both ABF1 binding sites have been shown to be transcriptional activators, as deletions and mutations at these sites has decreased transcriptional activity. ABF1 behaves as a transcriptional factor or activator for the ADH1 promoter.
mutable notes: No specific design considerations were dealt with.
mutable provenance: Source: NCBI GenBank Organism:Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K976003_sequence/1

BBa_K1486029
type: ComponentDefinition
persistent identity: BBa K1486029
display id: BBa_K1486029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486024
component: BBa_K1486028
sequence annotation: BBa_K1486028
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486029_sequence/1

BBa_K1486025
type: Component
persistent identity: component2417700
display id: component2417700
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2417697
display id: component2417697
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417704
display id: component2417704
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486024

BBa_K1486028
type: Component
persistent identity: component2417699
display id: component2417699
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486028

BBa_K1486028
type: SequenceAnnotation
persistent identity: annotation2417699
display id: annotation2417699
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
component: BBa_K1486028

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
type: Range
persistent identity: range2417699
display id: range2417699
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 40
end: 681
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417700
display id: annotation2417700
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
type: Range
persistent identity: range2417700
display id: range2417700
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 682
end: 869
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417704
display id: annotation2417704
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
type: Range
persistent identity: range2417704
display id: range2417704
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 870
end: 2226
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417697
display id: annotation2417697
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
type: Range
persistent identity: range2417697
display id: range2417697
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 1
end: 39
orientation: inline

BBa_K110014
type: ComponentDefinition
persistent identity: BBa K110014
display id: BBa_K110014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
mutable notes: This was taken from SGD
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110014_sequence/1

BBa_J24813
type: ComponentDefinition
persistent identity: BBa J24813
display id: BBa_J24813
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: URA3 Promoter from S. cerevisiae
creator: John Szymanski
created: 2008-05-01T11:00:00Z
modified: 2015-08-31T04:08:43Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
mutable notes: None
mutable provenance: NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J24813_sequence/1

BBa_K110007
type: ComponentDefinition
persistent identity: BBa K110007
display id: BBa_K110007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110007_sequence/1

BBa_K801020
type: ComponentDefinition
persistent identity: BBa K801020
display id: BBa_K801020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KlADH4 yeast promoter, ethanol inducible
creator: Simon Heinze
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1057_
m user id: 0
owner id: 9982
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is the ethanol inducible promoter controlling the KlADH4-gene of ''K. lactis''.
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''K. lactis'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: UASe
sequence annotation: SpeI-Site removed via quickchange
sequence annotation: possible transcription initiation region (Inr)
sequence annotation: STRE1 (Stress response element)
sequence annotation: HSE (Heat shock element)
sequence annotation: TATA-Box
sequence annotation: Rap1 protein binding region
sequence annotation: STRE4 (Stress response element)
sequence annotation: DCE-III
sequence annotation: KlADH4-promoter
sequence annotation: DCE-I
sequence annotation: STRE3 (Stress response element)
sequence annotation: Yap1 protein binding region
sequence annotation: STRE2 (Stress response element)
sequence annotation: STRE5 (Stress response element)
sequence annotation: DCE I
sequence: https://synbiohub.org/public/igem/BBa_K801020_sequence/1

UASe
type: SequenceAnnotation
persistent identity: annotation2195946
display id: annotation2195946
version: 1
top level: BBa_K801020
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195946/range2195946/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195946/range2195946/1
type: Range
persistent identity: range2195946
display id: range2195946
version: 1
direction: direction/forward
top level: BBa_K801020
start: 11
end: 212
orientation: inline

SpeI-Site removed via quickchange
type: SequenceAnnotation
persistent identity: annotation2195956
display id: annotation2195956
version: 1
top level: BBa_K801020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195956/range2195956/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195956/range2195956/1
type: Range
persistent identity: range2195956
display id: range2195956
version: 1
direction: direction/forward
top level: BBa_K801020
start: 757
end: 761
orientation: inline

possible transcription initiation region (Inr)
type: SequenceAnnotation
persistent identity: annotation2195960
display id: annotation2195960
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195960/range2195960/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195960/range2195960/1
type: Range
persistent identity: range2195960
display id: range2195960
version: 1
direction: direction/forward
top level: BBa_K801020
start: 867
end: 874
orientation: inline

STRE1 (Stress response element)
type: SequenceAnnotation
persistent identity: annotation2195954
display id: annotation2195954
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195954/range2195954/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195954/range2195954/1
type: Range
persistent identity: range2195954
display id: range2195954
version: 1
direction: direction/forward
top level: BBa_K801020
start: 181
end: 185
orientation: inline

HSE (Heat shock element)
type: SequenceAnnotation
persistent identity: annotation2195955
display id: annotation2195955
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195955/range2195955/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195955/range2195955/1
type: Range
persistent identity: range2195955
display id: range2195955
version: 1
direction: direction/forward
top level: BBa_K801020
start: 199
end: 210
orientation: inline

TATA-Box
type: SequenceAnnotation
persistent identity: annotation2195957
display id: annotation2195957
version: 1
top level: BBa_K801020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195957/range2195957/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195957/range2195957/1
type: Range
persistent identity: range2195957
display id: range2195957
version: 1
direction: direction/forward
top level: BBa_K801020
start: 833
end: 838
orientation: inline

Rap1 protein binding region
type: SequenceAnnotation
persistent identity: annotation2195948
display id: annotation2195948
version: 1
top level: BBa_K801020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195948/range2195948/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195948/range2195948/1
type: Range
persistent identity: range2195948
display id: range2195948
version: 1
direction: direction/forward
top level: BBa_K801020
start: 34
end: 46
orientation: inline

STRE4 (Stress response element)
type: SequenceAnnotation
persistent identity: annotation2195950
display id: annotation2195950
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195950/range2195950/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195950/range2195950/1
type: Range
persistent identity: range2195950
display id: range2195950
version: 1
direction: direction/forward
top level: BBa_K801020
start: 51
end: 55
orientation: inline

DCE-III
type: SequenceAnnotation
persistent identity: annotation2195963
display id: annotation2195963
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195963/range2195963/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195963/range2195963/1
type: Range
persistent identity: range2195963
display id: range2195963
version: 1
direction: direction/forward
top level: BBa_K801020
start: 908
end: 910
orientation: inline

possible transcription initiation region (Inr)
type: SequenceAnnotation
persistent identity: annotation2195959
display id: annotation2195959
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195959/range2195959/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195959/range2195959/1
type: Range
persistent identity: range2195959
display id: range2195959
version: 1
direction: direction/forward
top level: BBa_K801020
start: 859
end: 865
orientation: inline

KlADH4-promoter
type: SequenceAnnotation
persistent identity: annotation2195945
display id: annotation2195945
version: 1
top level: BBa_K801020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195945/range2195945/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195945/range2195945/1
type: Range
persistent identity: range2195945
display id: range2195945
version: 1
direction: direction/forward
top level: BBa_K801020
start: 1
end: 952
orientation: inline

DCE-I
type: SequenceAnnotation
persistent identity: annotation2195961
display id: annotation2195961
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195961/range2195961/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195961/range2195961/1
type: Range
persistent identity: range2195961
display id: range2195961
version: 1
direction: direction/forward
top level: BBa_K801020
start: 877
end: 880
orientation: inline

STRE3 (Stress response element)
type: SequenceAnnotation
persistent identity: annotation2195952
display id: annotation2195952
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195952/range2195952/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195952/range2195952/1
type: Range
persistent identity: range2195952
display id: range2195952
version: 1
direction: direction/forward
top level: BBa_K801020
start: 161
end: 165
orientation: inline

HSE (Heat shock element)
type: SequenceAnnotation
persistent identity: annotation2195951
display id: annotation2195951
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195951/range2195951/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195951/range2195951/1
type: Range
persistent identity: range2195951
display id: range2195951
version: 1
direction: direction/forward
top level: BBa_K801020
start: 110
end: 117
orientation: inline

Yap1 protein binding region
type: SequenceAnnotation
persistent identity: annotation2195949
display id: annotation2195949
version: 1
top level: BBa_K801020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195949/range2195949/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195949/range2195949/1
type: Range
persistent identity: range2195949
display id: range2195949
version: 1
direction: direction/forward
top level: BBa_K801020
start: 44
end: 50
orientation: inline

STRE2 (Stress response element)
type: SequenceAnnotation
persistent identity: annotation2195953
display id: annotation2195953
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195953/range2195953/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195953/range2195953/1
type: Range
persistent identity: range2195953
display id: range2195953
version: 1
direction: direction/forward
top level: BBa_K801020
start: 170
end: 173
orientation: inline

STRE5 (Stress response element)
type: SequenceAnnotation
persistent identity: annotation2195947
display id: annotation2195947
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195947/range2195947/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195947/range2195947/1
type: Range
persistent identity: range2195947
display id: range2195947
version: 1
direction: direction/forward
top level: BBa_K801020
start: 20
end: 24
orientation: inline

possible transcription initiation region (Inr)
type: SequenceAnnotation
persistent identity: annotation2195958
display id: annotation2195958
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195958/range2195958/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195958/range2195958/1
type: Range
persistent identity: range2195958
display id: range2195958
version: 1
direction: direction/forward
top level: BBa_K801020
start: 852
end: 857
orientation: inline

DCE I
type: SequenceAnnotation
persistent identity: annotation2195962
display id: annotation2195962
version: 1
top level: BBa_K801020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801020/annotation2195962/range2195962/1

https://synbiohub.org/public/igem/BBa_K801020/annotation2195962/range2195962/1
type: Range
persistent identity: range2195962
display id: range2195962
version: 1
direction: direction/forward
top level: BBa_K801020
start: 899
end: 901
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K165004
display id: BBa_K165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the mCherry fluorescent protein, yeast-codon optimized and biobricked in the silver lab BioFusion standard for use in fusion proteins.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165004_sequence/1

BBa_K165042
type: ComponentDefinition
persistent identity: BBa K165042
display id: BBa_K165042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + MET25 inducible yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165000
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165042_sequence/1

BBa_K165000
type: Component
persistent identity: component1996563
display id: component1996563
version: 1
top level: BBa_K165042
access: public
definition: BBa_K165000

BBa_K165012
type: Component
persistent identity: component1996562
display id: component1996562
version: 1
top level: BBa_K165042
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996562
display id: annotation1996562
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
type: Range
persistent identity: range1996562
display id: range1996562
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 1
end: 127
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996563
display id: annotation1996563
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
type: Range
persistent identity: range1996563
display id: range1996563
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 136
end: 522
orientation: inline

BBa_K165022
type: ComponentDefinition
persistent identity: BBa K165022
display id: BBa_K165022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV synthetic activator (untagged)
creator: John Szymanski
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the Zif268-HIV DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Zif268-HIV binding sites, activating transcription downstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K165022_sequence/1

BBa_K1486028
type: ComponentDefinition
persistent identity: BBa K1486028
display id: BBa_K1486028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized sfGFP N-terminus (1-214)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: split N term of sfGFP To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: yeast optimized sfGFP N-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486028_sequence/1

yeast optimized sfGFP N-terminus
type: SequenceAnnotation
persistent identity: annotation2417081
display id: annotation2417081
version: 1
top level: BBa_K1486028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1

https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1
type: Range
persistent identity: range2417081
display id: range2417081
version: 1
direction: direction/forward
top level: BBa_K1486028
start: 1
end: 642
orientation: inline

BBa_K801098
type: ComponentDefinition
persistent identity: BBa K801098
display id: BBa_K801098
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: O-methyltransferase 1 (OMT1) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: O-methyltransferase 1 (OMT1) coding region from ''Humulus lupulus''
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nucleotide/167613934/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: OMT
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801098_sequence/1

OMT
type: SequenceAnnotation
persistent identity: annotation2202174
display id: annotation2202174
version: 1
top level: BBa_K801098
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801098/annotation2202174/range2202174/1

https://synbiohub.org/public/igem/BBa_K801098/annotation2202174/range2202174/1
type: Range
persistent identity: range2202174
display id: range2202174
version: 1
direction: direction/forward
top level: BBa_K801098
start: 1
end: 1056
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202173
display id: annotation2202173
version: 1
top level: BBa_K801098
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801098/annotation2202173/range2202173/1

https://synbiohub.org/public/igem/BBa_K801098/annotation2202173/range2202173/1
type: Range
persistent identity: range2202173
display id: range2202173
version: 1
direction: direction/forward
top level: BBa_K801098
start: 1
end: 3
orientation: inline

BBa_K950006
type: ComponentDefinition
persistent identity: BBa K950006
display id: BBa_K950006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: membrane progesterone receptor (Danio rerio)
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Membrane progesterone receptor of Danio rerio. Upon ligand binding, the receptor represses expression of Fet3 in yeast.
mutable notes: restriction sites removed and codon usage optimized for yeast
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/AAN78115.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: mPR Danio rerio
sequence: https://synbiohub.org/public/igem/BBa_K950006_sequence/1

mPR Danio rerio
type: SequenceAnnotation
persistent identity: annotation2179275
display id: annotation2179275
version: 1
top level: BBa_K950006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950006/annotation2179275/range2179275/1

https://synbiohub.org/public/igem/BBa_K950006/annotation2179275/range2179275/1
type: Range
persistent identity: range2179275
display id: range2179275
version: 1
direction: direction/forward
top level: BBa_K950006
start: 1
end: 1065
orientation: inline

BBa_K1462020
type: ComponentDefinition
persistent identity: BBa K1462020
display id: BBa_K1462020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erg10
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462020_sequence/1

BBa_K1680024
type: ComponentDefinition
persistent identity: BBa K1680024
display id: BBa_K1680024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:22:01Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly with RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680004
sequence annotation: K1680006
sequence annotation: K1680007
sequence annotation: K1680003
sequence: https://synbiohub.org/public/igem/BBa_K1680024_sequence/1

K1680004
type: SequenceAnnotation
persistent identity: annotation2477390
display id: annotation2477390
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1
type: Range
persistent identity: range2477390
display id: range2477390
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1
end: 24
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477393
display id: annotation2477393
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1
type: Range
persistent identity: range2477393
display id: range2477393
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 31
end: 699
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477392
display id: annotation2477392
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1
type: Range
persistent identity: range2477392
display id: range2477392
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 745
end: 1773
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477395
display id: annotation2477395
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1
type: Range
persistent identity: range2477395
display id: range2477395
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 700
end: 744
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477394
display id: annotation2477394
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1
type: Range
persistent identity: range2477394
display id: range2477394
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1818
end: 2487
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477396
display id: annotation2477396
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1
type: Range
persistent identity: range2477396
display id: range2477396
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1774
end: 1817
orientation: inline

BBa_K165030
type: ComponentDefinition
persistent identity: BBa K165030
display id: BBa_K165030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Zif268-HIV binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Zif268-HIV binding sites (3)
sequence: https://synbiohub.org/public/igem/BBa_K165030_sequence/1

Zif268-HIV binding sites (3)
type: SequenceAnnotation
persistent identity: annotation1994037
display id: annotation1994037
version: 1
top level: BBa_K165030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1

https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1
type: Range
persistent identity: range1994037
display id: range1994037
version: 1
direction: direction/forward
top level: BBa_K165030
start: 1
end: 46
orientation: inline

BBa_K106698
type: ComponentDefinition
persistent identity: BBa K106698
display id: BBa_K106698
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor for AarI AD parts, with 8X LexA operators directly upstream of the medium strong Cyc1 promoter.
mutable notes: LexA Ops were cloned non-directionally into the PspOMI site. Inner site is broken, thus the cassette can be subcloned from this plasmid by PspOMI/SacI digestion (for example, to change markers).
mutable provenance: Modification of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: ampicillin marker promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: 8X LexA Operators
sequence annotation: LEU2 marker
sequence annotation: lacZalpha reporter
sequence annotation: T3 promoter
sequence annotation: D overhang
sequence annotation: pBR322 origin
sequence annotation: f1 origin
sequence annotation: ampicillin resistance marker
sequence annotation: CEN ARS
sequence annotation: Adh1 terminator
sequence annotation: Fig1 Promoter
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K106698_sequence/1

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999743
display id: annotation1999743
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1
type: Range
persistent identity: range1999743
display id: range1999743
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999747
display id: annotation1999747
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1
type: Range
persistent identity: range1999747
display id: range1999747
version: 1
direction: direction/forward
top level: BBa_K106698
start: 7309
end: 7312
orientation: inline

ampicillin marker promoter
type: SequenceAnnotation
persistent identity: annotation1999741
display id: annotation1999741
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1
type: Range
persistent identity: range1999741
display id: range1999741
version: 1
direction: direction/forward
top level: BBa_K106698
start: 2983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999746
display id: annotation1999746
version: 1
top level: BBa_K106698
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1
type: Range
persistent identity: range1999746
display id: range1999746
version: 1
direction: direction/forward
top level: BBa_K106698
start: 11
end: 21
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995345
display id: annotation1995345
version: 1
top level: BBa_K106698
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1
type: Range
persistent identity: range1995345
display id: range1995345
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6132
end: 6299
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995348
display id: annotation1995348
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1
type: Range
persistent identity: range1995348
display id: range1995348
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1570
end: 2676
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999739
display id: annotation1999739
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1
type: Range
persistent identity: range1999739
display id: range1999739
version: 1
direction: direction/forward
top level: BBa_K106698
start: 246
end: 405
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999744
display id: annotation1999744
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1
type: Range
persistent identity: range1999744
display id: range1999744
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6095
end: 6114
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999745
display id: annotation1999745
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1
type: Range
persistent identity: range1999745
display id: range1999745
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1
end: 4
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999742
display id: annotation1999742
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1
type: Range
persistent identity: range1999742
display id: range1999742
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5068
end: 5687
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999740
display id: annotation1999740
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1
type: Range
persistent identity: range1999740
display id: range1999740
version: 1
direction: direction/forward
top level: BBa_K106698
start: 427
end: 733
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995344
display id: annotation1995344
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1
type: Range
persistent identity: range1995344
display id: range1995344
version: 1
direction: direction/forward
top level: BBa_K106698
start: 4053
end: 4913
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995343
display id: annotation1995343
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1
type: Range
persistent identity: range1995343
display id: range1995343
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 3403
end: 3921
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995347
display id: annotation1995347
version: 1
top level: BBa_K106698
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1
type: Range
persistent identity: range1995347
display id: range1995347
version: 1
direction: direction/forward
top level: BBa_K106698
start: 22
end: 215
orientation: inline

Fig1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995346
display id: annotation1995346
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1
type: Range
persistent identity: range1995346
display id: range1995346
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6300
end: 7309
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999738
display id: annotation1999738
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1
type: Range
persistent identity: range1999738
display id: range1999738
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 224
end: 242
orientation: inline

BBa_K1072010
type: ComponentDefinition
persistent identity: BBa K1072010
display id: BBa_K1072010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Odr-10(diacetyl)GPCR with optimized codon
creator: Junqi kuang, Xihao liao and Jianhui gong
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ.
mutable notes: As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully.
mutable provenance: Originate from the C. elegans.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Odr-10 coding region
sequence: https://synbiohub.org/public/igem/BBa_K1072010_sequence/1

Odr-10 coding region
type: SequenceAnnotation
persistent identity: annotation2357697
display id: annotation2357697
version: 1
top level: BBa_K1072010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1

https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1
type: Range
persistent identity: range2357697
display id: range2357697
version: 1
direction: direction/forward
top level: BBa_K1072010
start: 1
end: 1020
orientation: inline

BBa_K586000
type: ComponentDefinition
persistent identity: BBa K586000
display id: BBa_K586000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cup-1 Heavy metal sensor
creator: Chris Thompson
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _757_
m user id: 0
owner id: 9336
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
mutable notes: None
mutable provenance: Comes from genomic DNA of saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Cup-1
sequence: https://synbiohub.org/public/igem/BBa_K586000_sequence/1

Cup-1
type: SequenceAnnotation
persistent identity: annotation2122521
display id: annotation2122521
version: 1
top level: BBa_K586000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1

https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1
type: Range
persistent identity: range2122521
display id: range2122521
version: 1
direction: direction/forward
top level: BBa_K586000
start: 1
end: 186
orientation: inline

BBa_K1861060
type: ComponentDefinition
persistent identity: BBa K1861060
display id: BBa_K1861060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE2 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Why can't I edit these descriptions?
mutable notes: Coming soon, if I am allowed to edit this.
mutable provenance: It keeps saying I have no permission to edit this.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861601
component: BBa_K1159201
component: BBa_K1861102
component: BBa_K392003
component: BBa_K1861104
component: BBa_K1861101
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861102
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861601
sequence: https://synbiohub.org/public/igem/BBa_K1861060_sequence/1

BBa_K1861601
type: Component
persistent identity: component2475042
display id: component2475042
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861601/1

BBa_K1159201
type: Component
persistent identity: component2475045
display id: component2475045
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861102
type: Component
persistent identity: component2475041
display id: component2475041
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K392003
type: Component
persistent identity: component2475047
display id: component2475047
version: 1
top level: BBa_K1861060
access: public
definition: BBa_K392003

BBa_K1861104
type: Component
persistent identity: component2475046
display id: component2475046
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861101
type: Component
persistent identity: component2475040
display id: component2475040
version: 1
top level: BBa_K1861060
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2475046
display id: annotation2475046
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475046/range2475046/1
type: Range
persistent identity: range2475046
display id: range2475046
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2515
end: 2523
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2475045
display id: annotation2475045
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475045/range2475045/1
type: Range
persistent identity: range2475045
display id: range2475045
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2476
end: 2514
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2475040
display id: annotation2475040
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475040/range2475040/1
type: Range
persistent identity: range2475040
display id: range2475040
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 1
end: 238
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2475041
display id: annotation2475041
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475041/range2475041/1
type: Range
persistent identity: range2475041
display id: range2475041
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 239
end: 265
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2475047
display id: annotation2475047
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475047/range2475047/1
type: Range
persistent identity: range2475047
display id: range2475047
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 2524
end: 2652
orientation: inline

BBa_K1861601
type: SequenceAnnotation
persistent identity: annotation2475042
display id: annotation2475042
version: 1
top level: BBa_K1861060
location: https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
component: BBa_K1861601

https://synbiohub.org/public/igem/BBa_K1861060/annotation2475042/range2475042/1
type: Range
persistent identity: range2475042
display id: range2475042
version: 1
direction: direction/reverse
top level: BBa_K1861060
start: 266
end: 2475
orientation: inline

BBa_K950002
type: ComponentDefinition
persistent identity: BBa K950002
display id: BBa_K950002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast anb1 promotor
creator: Jakob Matthes
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the promotor of yeast anb1 gene. Panb1 is a target of the rox1 repressor.
mutable notes: genomic sequence was not altered
mutable provenance: sequence and information from http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=SUC2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: rox1 binding site
sequence: https://synbiohub.org/public/igem/BBa_K950002_sequence/1

rox1 binding site
type: SequenceAnnotation
persistent identity: annotation2179270
display id: annotation2179270
version: 1
top level: BBa_K950002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950002/annotation2179270/range2179270/1

https://synbiohub.org/public/igem/BBa_K950002/annotation2179270/range2179270/1
type: Range
persistent identity: range2179270
display id: range2179270
version: 1
direction: direction/forward
top level: BBa_K950002
start: 186
end: 191
orientation: inline

rox1 binding site
type: SequenceAnnotation
persistent identity: annotation2179269
display id: annotation2179269
version: 1
top level: BBa_K950002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950002/annotation2179269/range2179269/1

https://synbiohub.org/public/igem/BBa_K950002/annotation2179269/range2179269/1
type: Range
persistent identity: range2179269
display id: range2179269
version: 1
direction: direction/forward
top level: BBa_K950002
start: 88
end: 93
orientation: inline

BBa_K1728023
type: ComponentDefinition
persistent identity: BBa K1728023
display id: BBa_K1728023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LUCY rhodopsin signalling peptide
creator: Wan-Yun Chiu, Ming-Fei Hsieh
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T01:36:59Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2149_
m user id: 18379
owner id: 18379
owning group id: 9
status: status/Planning
bookmark: false
mutable description: LUCY rhodopsin signalling peptide
mutable notes: No
mutable provenance: Order the DNA part from synthesis company
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1728020
component: BBa_K1728022
sequence annotation: BBa_K1728022
sequence annotation: BBa_K1728020
sequence: https://synbiohub.org/public/igem/BBa_K1728023_sequence/1

BBa_K1728020
type: Component
persistent identity: component2464320
display id: component2464320
version: 1
top level: BBa_K1728023
access: public
definition: BBa_K1728020

BBa_K1728022
type: Component
persistent identity: component2464321
display id: component2464321
version: 1
top level: BBa_K1728023
access: public
definition: BBa_K1728022

BBa_K1728022
type: SequenceAnnotation
persistent identity: annotation2464321
display id: annotation2464321
version: 1
top level: BBa_K1728023
location: https://synbiohub.org/public/igem/BBa_K1728023/annotation2464321/range2464321/1
component: BBa_K1728022

https://synbiohub.org/public/igem/BBa_K1728023/annotation2464321/range2464321/1
type: Range
persistent identity: range2464321
display id: range2464321
version: 1
direction: direction/reverse
top level: BBa_K1728023
start: 61
end: 117
orientation: inline

BBa_K1728020
type: SequenceAnnotation
persistent identity: annotation2464320
display id: annotation2464320
version: 1
top level: BBa_K1728023
location: https://synbiohub.org/public/igem/BBa_K1728023/annotation2464320/range2464320/1
component: BBa_K1728020

https://synbiohub.org/public/igem/BBa_K1728023/annotation2464320/range2464320/1
type: Range
persistent identity: range2464320
display id: range2464320
version: 1
direction: direction/reverse
top level: BBa_K1728023
start: 1
end: 60
orientation: inline

BBa_K105029
type: ComponentDefinition
persistent identity: BBa K105029
display id: BBa_K105029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc43 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105029_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989837
display id: annotation1989837
version: 1
top level: BBa_K105029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1
type: Range
persistent identity: range1989837
display id: range1989837
version: 1
direction: direction/forward
top level: BBa_K105029
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989838
display id: annotation1989838
version: 1
top level: BBa_K105029
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1
type: Range
persistent identity: range1989838
display id: range1989838
version: 1
direction: direction/forward
top level: BBa_K105029
start: 23
end: 23
orientation: inline

BBa_K1907009
type: ComponentDefinition
persistent identity: BBa K1907009
display id: BBa_K1907009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CTT1 promoter + Venus YFP
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T03:16:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1907000
component: BBa_K1907003
sequence annotation: BBa_K1907003
sequence annotation: BBa_K1907000
sequence: https://synbiohub.org/public/igem/BBa_K1907009_sequence/1

BBa_K1907000
type: Component
persistent identity: component2522682
display id: component2522682
version: 1
top level: BBa_K1907009
access: public
definition: Venus

BBa_K1907003
type: Component
persistent identity: component2522681
display id: component2522681
version: 1
top level: BBa_K1907009
access: public
definition: BBa_K1907003

BBa_K1907003
type: SequenceAnnotation
persistent identity: annotation2522681
display id: annotation2522681
version: 1
top level: BBa_K1907009
location: https://synbiohub.org/public/igem/BBa_K1907009/annotation2522681/range2522681/1
component: BBa_K1907003

https://synbiohub.org/public/igem/BBa_K1907009/annotation2522681/range2522681/1
type: Range
persistent identity: range2522681
display id: range2522681
version: 1
direction: direction/reverse
top level: BBa_K1907009
start: 1
end: 924
orientation: inline

BBa_K1907000
type: SequenceAnnotation
persistent identity: annotation2522682
display id: annotation2522682
version: 1
top level: BBa_K1907009
location: https://synbiohub.org/public/igem/BBa_K1907009/annotation2522682/range2522682/1
component: BBa_K1907000

https://synbiohub.org/public/igem/BBa_K1907009/annotation2522682/range2522682/1
type: Range
persistent identity: range2522682
display id: range2522682
version: 1
direction: direction/reverse
top level: BBa_K1907009
start: 931
end: 1647
orientation: inline

BBa_K1611002
type: ComponentDefinition
persistent identity: BBa K1611002
display id: BBa_K1611002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OVA1
creator: Frederic Ros
created: 2015-09-14T11:00:00Z
modified: 2015-12-17T01:04:22Z
discontinued: false
dominant: false
group u list: _2028_
m user id: 4206
owner id: 28291
owning group id: 9
status: status/Planning
bookmark: false
mutable description: OVA1 is an antigen expressed in tumor melanocytes cell line B16-OVA1. In the immune system, it is presented by the MHC-I to activate T-CD8 cells. It activated CD8+ in vivo against OVA1 in C57BL/6 mice, as tetramer CD8+ assays demonstrated.
mutable notes: Nothing to signal.
mutable provenance: OVAI was synthesized by the epitope SIINFEKL + surrounding residues to allow better processing by the immunoproteasome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1611002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2456828
display id: annotation2456828
version: 1
top level: BBa_K1611002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1611002/annotation2456828/range2456828/1

https://synbiohub.org/public/igem/BBa_K1611002/annotation2456828/range2456828/1
type: Range
persistent identity: range2456828
display id: range2456828
version: 1
direction: direction/forward
top level: BBa_K1611002
start: 1
end: 63
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2456827
display id: annotation2456827
version: 1
top level: BBa_K1611002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1611002/annotation2456827/range2456827/1

https://synbiohub.org/public/igem/BBa_K1611002/annotation2456827/range2456827/1
type: Range
persistent identity: range2456827
display id: range2456827
version: 1
direction: direction/forward
top level: BBa_K1611002
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2456829
display id: annotation2456829
version: 1
top level: BBa_K1611002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1611002/annotation2456829/range2456829/1

https://synbiohub.org/public/igem/BBa_K1611002/annotation2456829/range2456829/1
type: Range
persistent identity: range2456829
display id: range2456829
version: 1
direction: direction/forward
top level: BBa_K1611002
start: 61
end: 66
orientation: inline

BBa_M36527
type: ComponentDefinition
persistent identity: BBa M36527
display id: BBa_M36527
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactose inducible yeast promoter
creator: Valerie Wesley Scott
created: 2015-10-22T11:00:00Z
modified: 2016-02-10T02:35:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 4206
owner id: 29107
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In the presence of LacI, this sequence represses gene expression. Expression can be induced by the addition of lactose or IPTG, which binds to LacI, removing it from the DNA.
mutable notes: Had to ensure that it would work in yeast and where to put this with regards to the Kozak sequence and protein coding region. Ended up being placed directly before the Kozak sequence.
mutable provenance: Was extracted from the BBa_K1164004 part on iGem's Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M36527_sequence/1

BBa_K105027
type: ComponentDefinition
persistent identity: BBa K105027
display id: BBa_K105027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc100 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
mutable notes: This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
mutable provenance: Amplification from the plasmid pAH3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K105027_sequence/1

TATA box
type: SequenceAnnotation
persistent identity: annotation1989774
display id: annotation1989774
version: 1
top level: BBa_K105027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1

https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1
type: Range
persistent identity: range1989774
display id: range1989774
version: 1
direction: direction/forward
top level: BBa_K105027
start: 17
end: 23
orientation: inline

BBa_K165093
type: ComponentDefinition
persistent identity: BBa K165093
display id: BBa_K165093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + TEF2 + Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165014
component: BBa_K165002
component: BBa_K165011
component: BBa_K165037
component: BBa_K165018
component: BBa_K165003
component: BBa_K165007
sequence annotation: BBa_K165014
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165037
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165093_sequence/1

BBa_K165014
type: Component
persistent identity: component1997333
display id: component1997333
version: 1
top level: BBa_K165093
access: public
definition: cfpx2

BBa_K165002
type: Component
persistent identity: component1997330
display id: component1997330
version: 1
top level: BBa_K165093
access: public
definition: BBa_K165002

BBa_K165011
type: Component
persistent identity: component1997328
display id: component1997328
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: Component
persistent identity: component1997329
display id: component1997329
version: 1
top level: BBa_K165093
access: public
definition: pTEF

BBa_K165018
type: Component
persistent identity: component1997334
display id: component1997334
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165003
type: Component
persistent identity: component1997331
display id: component1997331
version: 1
top level: BBa_K165093
access: public
definition: Sin3

BBa_K165007
type: Component
persistent identity: component1997332
display id: component1997332
version: 1
top level: BBa_K165093
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997333
display id: annotation1997333
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997333/range1997333/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165093/annotation1997333/range1997333/1
type: Range
persistent identity: range1997333
display id: range1997333
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 3844
end: 5337
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997334
display id: annotation1997334
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997334/range1997334/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165093/annotation1997334/range1997334/1
type: Range
persistent identity: range1997334
display id: range1997334
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 5346
end: 5598
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997332
display id: annotation1997332
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997332/range1997332/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165093/annotation1997332/range1997332/1
type: Range
persistent identity: range1997332
display id: range1997332
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 3311
end: 3835
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997328
display id: annotation1997328
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997328/range1997328/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165093/annotation1997328/range1997328/1
type: Range
persistent identity: range1997328
display id: range1997328
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 1
end: 46
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997329
display id: annotation1997329
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997329/range1997329/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165093/annotation1997329/range1997329/1
type: Range
persistent identity: range1997329
display id: range1997329
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 55
end: 457
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997330
display id: annotation1997330
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997330/range1997330/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165093/annotation1997330/range1997330/1
type: Range
persistent identity: range1997330
display id: range1997330
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 466
end: 483
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997331
display id: annotation1997331
version: 1
top level: BBa_K165093
location: https://synbiohub.org/public/igem/BBa_K165093/annotation1997331/range1997331/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165093/annotation1997331/range1997331/1
type: Range
persistent identity: range1997331
display id: range1997331
version: 1
direction: direction/reverse
top level: BBa_K165093
start: 492
end: 3302
orientation: inline

BBa_K105030
type: ComponentDefinition
persistent identity: BBa K105030
display id: BBa_K105030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc28 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A -> T
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105030_sequence/1

A -> T
type: SequenceAnnotation
persistent identity: annotation1989840
display id: annotation1989840
version: 1
top level: BBa_K105030
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1
type: Range
persistent identity: range1989840
display id: range1989840
version: 1
direction: direction/forward
top level: BBa_K105030
start: 22
end: 22
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989839
display id: annotation1989839
version: 1
top level: BBa_K105030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1
type: Range
persistent identity: range1989839
display id: range1989839
version: 1
direction: direction/forward
top level: BBa_K105030
start: 17
end: 23
orientation: inline

BBa_K801000
type: ComponentDefinition
persistent identity: BBa K801000
display id: BBa_K801000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM100 yeast shuttle vector based on pYES2
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A yeast shuttle vector containing the following structural elements: * 2?? ori for replication in ''S. cerevisiae'' * URA3-gene for selection of transformed yeast via Uracil prototrophy * pUC ori for replication in ''E. coli'' * AmpR-gene for selection of transformed ''E. coli'' via Ampicillin resistance * f1 ori * MCS containing the RFC25 pre- and suffix for easy insertion of biobricks * following the MCS: CYC1 yeast transcriptional terminator Note: To use this backbone, a ''S. cerevisiae'' strain with Uracil-auxotrophy (such as strain INVSc1) is required for positive selection of transformants. Successfully transformed yeast cells will become prototrophic for uracil and can be selected on Agar-plates deficient of uracil.
mutable notes: -
mutable provenance: Based on invitrogen's pYES2-plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: URA3
sequence annotation: RFC25 MCS
sequence annotation: Strep-tag II
sequence annotation: 2 micron (??) origin
sequence annotation: T7-Promoter
sequence annotation: CYC1-Terminator
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801000_sequence/1

URA3
type: SequenceAnnotation
persistent identity: annotation2202325
display id: annotation2202325
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1
type: Range
persistent identity: range2202325
display id: range2202325
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 2274
end: 3381
orientation: inline

RFC25 MCS
type: SequenceAnnotation
persistent identity: annotation2202314
display id: annotation2202314
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1
type: Range
persistent identity: range2202314
display id: range2202314
version: 1
direction: direction/forward
top level: BBa_K801000
start: 36
end: 134
orientation: inline

Strep-tag II
type: SequenceAnnotation
persistent identity: annotation2202315
display id: annotation2202315
version: 1
top level: BBa_K801000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1
type: Range
persistent identity: range2202315
display id: range2202315
version: 1
direction: direction/forward
top level: BBa_K801000
start: 78
end: 101
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2202326
display id: annotation2202326
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1
type: Range
persistent identity: range2202326
display id: range2202326
version: 1
direction: direction/forward
top level: BBa_K801000
start: 3385
end: 4856
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2202258
display id: annotation2202258
version: 1
top level: BBa_K801000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1
type: Range
persistent identity: range2202258
display id: range2202258
version: 1
direction: direction/forward
top level: BBa_K801000
start: 4
end: 23
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202316
display id: annotation2202316
version: 1
top level: BBa_K801000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1
type: Range
persistent identity: range2202316
display id: range2202316
version: 1
direction: direction/forward
top level: BBa_K801000
start: 148
end: 396
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2202317
display id: annotation2202317
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1
type: Range
persistent identity: range2202317
display id: range2202317
version: 1
direction: direction/forward
top level: BBa_K801000
start: 578
end: 1251
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2202324
display id: annotation2202324
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1
type: Range
persistent identity: range2202324
display id: range2202324
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 1396
end: 2256
orientation: inline

BBa_K416008
type: ComponentDefinition
persistent identity: BBa K416008
display id: BBa_K416008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2 fused to eCFP
creator: Russell Durrett
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: When working correctly this reporter construct transcribes Aga2-eCFP from the Gal promoter. If the yeast strain is also expressing Aga1, then this fusion should be anchored onto the plasma membrane, allowing you to visualize eCFP as a halo around the yeast cell.
mutable notes: We considered fusing this reporter to a variety of promoters, but in the end chose pGAL because it gives a very high level of transcription and we could turn the reporter on and off
mutable provenance: This part comes from a mixture of biobricks that were received in the 2010 initial distribution and from parts that NYU iGEM 2010 made ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K105009
component: BBa_K416000
component: BBa_K416005
component: BBa_K416001
component: BBa_K106018
sequence annotation: BBa_K416000
sequence annotation: BBa_K106018
sequence annotation: BBa_K416001
sequence annotation: BBa_K416005
sequence annotation: BBa_K105009
sequence: https://synbiohub.org/public/igem/BBa_K416008_sequence/1

BBa_K105009
type: Component
persistent identity: component2110287
display id: component2110287
version: 1
top level: BBa_K416008
access: public
definition: ECFP

BBa_K416000
type: Component
persistent identity: component2110285
display id: component2110285
version: 1
top level: BBa_K416008
access: public
definition: BBa_K416000

BBa_K416005
type: Component
persistent identity: component2110283
display id: component2110283
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K416005/1

BBa_K416001
type: Component
persistent identity: component2110286
display id: component2110286
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K416001/1

BBa_K106018
type: Component
persistent identity: component2110288
display id: component2110288
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K106018/1

BBa_K416000
type: SequenceAnnotation
persistent identity: annotation2110285
display id: annotation2110285
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110285/range2110285/1
component: BBa_K416000

https://synbiohub.org/public/igem/BBa_K416008/annotation2110285/range2110285/1
type: Range
persistent identity: range2110285
display id: range2110285
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 582
end: 842
orientation: inline

BBa_K106018
type: SequenceAnnotation
persistent identity: annotation2110288
display id: annotation2110288
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110288/range2110288/1
component: BBa_K106018

https://synbiohub.org/public/igem/BBa_K416008/annotation2110288/range2110288/1
type: Range
persistent identity: range2110288
display id: range2110288
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 1656
end: 1835
orientation: inline

BBa_K416001
type: SequenceAnnotation
persistent identity: annotation2110286
display id: annotation2110286
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110286/range2110286/1
component: BBa_K416001

https://synbiohub.org/public/igem/BBa_K416008/annotation2110286/range2110286/1
type: Range
persistent identity: range2110286
display id: range2110286
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 851
end: 895
orientation: inline

BBa_K416005
type: SequenceAnnotation
persistent identity: annotation2110283
display id: annotation2110283
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110283/range2110283/1
component: BBa_K416005

https://synbiohub.org/public/igem/BBa_K416008/annotation2110283/range2110283/1
type: Range
persistent identity: range2110283
display id: range2110283
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 1
end: 575
orientation: inline

BBa_K105009
type: SequenceAnnotation
persistent identity: annotation2110287
display id: annotation2110287
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110287/range2110287/1
component: BBa_K105009

https://synbiohub.org/public/igem/BBa_K416008/annotation2110287/range2110287/1
type: Range
persistent identity: range2110287
display id: range2110287
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 904
end: 1647
orientation: inline

BBa_K106012
type: ComponentDefinition
persistent identity: BBa K106012
display id: BBa_K106012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sas2 histone acetyltransferase, Aar1 BD part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the gene coding for the Sas2 gene in yeast. It is a histone acetyltransferase (HAT).
mutable notes: Since this is a BD part, there is no ATG but it does include a stop codon. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106012_sequence/1

BBa_K165038
type: ComponentDefinition
persistent identity: BBa K165038
display id: BBa_K165038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + ADH1 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165012
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165038_sequence/1

BBa_K165015
type: Component
persistent identity: component1995513
display id: component1995513
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165015

BBa_K165012
type: Component
persistent identity: component1995512
display id: component1995512
version: 1
top level: BBa_K165038
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995513
display id: annotation1995513
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
type: Range
persistent identity: range1995513
display id: range1995513
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995512
display id: annotation1995512
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
type: Range
persistent identity: range1995512
display id: range1995512
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 1
end: 127
orientation: inline

BBa_K950003
type: ComponentDefinition
persistent identity: BBa K950003
display id: BBa_K950003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast suc2 promotor
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-09-25T08:34:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 26366
owner id: 13191
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Psuc2 promotor from yeast. Repressed by mig1
mutable notes: no restriction sites detected
mutable provenance: yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mig1B_BS
sequence annotation: Mig1A_BS
sequence: https://synbiohub.org/public/igem/BBa_K950003_sequence/1

Mig1B_BS
type: SequenceAnnotation
persistent identity: annotation2179272
display id: annotation2179272
version: 1
top level: BBa_K950003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950003/annotation2179272/range2179272/1

https://synbiohub.org/public/igem/BBa_K950003/annotation2179272/range2179272/1
type: Range
persistent identity: range2179272
display id: range2179272
version: 1
direction: direction/forward
top level: BBa_K950003
start: 250
end: 268
orientation: inline

Mig1A_BS
type: SequenceAnnotation
persistent identity: annotation2179271
display id: annotation2179271
version: 1
top level: BBa_K950003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950003/annotation2179271/range2179271/1

https://synbiohub.org/public/igem/BBa_K950003/annotation2179271/range2179271/1
type: Range
persistent identity: range2179271
display id: range2179271
version: 1
direction: direction/forward
top level: BBa_K950003
start: 200
end: 213
orientation: inline

BBa_K950007
type: ComponentDefinition
persistent identity: BBa K950007
display id: BBa_K950007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: membrane progesterone receptor (Xenopus laevis)
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Membrane progesterone receptor of Xenopus laevis. Upon ligand binding, the receptor represses expression of Fet3 in yeast.
mutable notes: restriction sites removed and codon usage optimized for yeast
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/AAN78113.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: mPR Xenopus laevis
sequence: https://synbiohub.org/public/igem/BBa_K950007_sequence/1

mPR Xenopus laevis
type: SequenceAnnotation
persistent identity: annotation2179276
display id: annotation2179276
version: 1
top level: BBa_K950007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950007/annotation2179276/range2179276/1

https://synbiohub.org/public/igem/BBa_K950007/annotation2179276/range2179276/1
type: Range
persistent identity: range2179276
display id: range2179276
version: 1
direction: direction/forward
top level: BBa_K950007
start: 1
end: 1062
orientation: inline

BBa_K801093
type: ComponentDefinition
persistent identity: BBa K801093
display id: BBa_K801093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate--coenzyme A ligase (4CL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: 4CL
sequence: https://synbiohub.org/public/igem/BBa_K801093_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202140
display id: annotation2202140
version: 1
top level: BBa_K801093
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
type: Range
persistent identity: range2202140
display id: range2202140
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202141
display id: annotation2202141
version: 1
top level: BBa_K801093
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
type: Range
persistent identity: range2202141
display id: range2202141
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1684
end: 1686
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2202143
display id: annotation2202143
version: 1
top level: BBa_K801093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
type: Range
persistent identity: range2202143
display id: range2202143
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 1683
orientation: inline

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: designed yeast Kozak sequence
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: consensus Kozak sequence and start codon
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
direction: direction/forward
top level: Kozak & st
start: 13
end: 15
orientation: inline

BBa_K1486025
type: ComponentDefinition
persistent identity: BBa K1486025
display id: BBa_K1486025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 Terminator
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Adh1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486025_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2418776
display id: annotation2418776
version: 1
top level: BBa_K1486025
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1

https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1
type: Range
persistent identity: range2418776
display id: range2418776
version: 1
direction: direction/forward
top level: BBa_K1486025
start: 1
end: 188
orientation: inline

BBa_K1486031
type: ComponentDefinition
persistent identity: BBa K1486031
display id: BBa_K1486031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaUra3 selection marker
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF terminator
sequence annotation: Ura3
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486031_sequence/1

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2417787
display id: annotation2417787
version: 1
top level: BBa_K1486031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1
type: Range
persistent identity: range2417787
display id: range2417787
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1162
end: 1360
orientation: inline

Ura3
type: SequenceAnnotation
persistent identity: annotation2417786
display id: annotation2417786
version: 1
top level: BBa_K1486031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1
type: Range
persistent identity: range2417786
display id: range2417786
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 345
end: 1157
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2417785
display id: annotation2417785
version: 1
top level: BBa_K1486031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1
type: Range
persistent identity: range2417785
display id: range2417785
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1
end: 344
orientation: inline

BBa_K801030
type: ComponentDefinition
persistent identity: BBa K801030
display id: BBa_K801030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SV40NLS nuclear localization sequence
mutable notes: -
mutable provenance: designed by oligo hybridisation based on plasmid "pGADT7 AD" from Clontech, w/ RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence: https://synbiohub.org/public/igem/BBa_K801030_sequence/1

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2198843
display id: annotation2198843
version: 1
top level: BBa_K801030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1

https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
type: Range
persistent identity: range2198843
display id: range2198843
version: 1
direction: direction/forward
top level: BBa_K801030
start: 22
end: 42
orientation: inline

BBa_K1728021
type: ComponentDefinition
persistent identity: BBa K1728021
display id: BBa_K1728021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chemokine receptor 1 (CXCR1)
creator: Wan-Yun Chiu, Ming-Fei Hsieh
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T01:22:27Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2149_
m user id: 18379
owner id: 18379
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Chemokine receptor 1 (CXCR1)
mutable notes: Codon optizimation for yeast
mutable provenance: Human genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1728021_sequence/1

BBa_M31201
type: ComponentDefinition
persistent identity: BBa M31201
display id: BBa_M31201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast CLB1 promoter region, G2/M cell cycle specific
creator: Katherine Aull
created: 2008-05-13T11:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 624
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
mutable notes: Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
mutable provenance: Yeast consensus sequence from SGD chrVII:703140..703639
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31201_sequence/1

MFa1-Secre
type: ComponentDefinition
persistent identity: BBa K792002
display id: BBa_K792002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretion tag from yeast α-factor mating pheromone (MFα1)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the secretion signal peptide for the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows for the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.
mutable notes: None
mutable provenance: Prepro-a-factorHas a Cleavable Signal Sequence [Water et al 1987]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: secretion tag
sequence: https://synbiohub.org/public/igem/BBa_K792002_sequence/1

secretion tag
type: SequenceAnnotation
persistent identity: annotation2197873
display id: annotation2197873
version: 1
top level: MFa1-Secre
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K792002/annotation2197873/range2197873/1

https://synbiohub.org/public/igem/BBa_K792002/annotation2197873/range2197873/1
type: Range
persistent identity: range2197873
display id: range2197873
version: 1
direction: direction/forward
top level: MFa1-Secre
start: 1
end: 54
orientation: inline

MlrA
type: ComponentDefinition
persistent identity: BBa K1907001
display id: BBa_K1907001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Microcystinase (MlrA) for S. cerevisiae
creator: Pihla Savola
created: 2016-10-11T11:00:00Z
modified: 2016-10-19T12:43:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29559
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: - - - - - - - - -
mutable notes: -- -- --
mutable provenance: -- -- --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-tag
sequence annotation: Linker
sequence annotation: MlrA CDS
sequence: https://synbiohub.org/public/igem/BBa_K1907001_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2522064
display id: annotation2522064
version: 1
top level: MlrA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1907001/annotation2522064/range2522064/1

https://synbiohub.org/public/igem/BBa_K1907001/annotation2522064/range2522064/1
type: Range
persistent identity: range2522064
display id: range2522064
version: 1
direction: direction/forward
top level: MlrA
start: 1018
end: 1035
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2522063
display id: annotation2522063
version: 1
top level: MlrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1907001/annotation2522063/range2522063/1

https://synbiohub.org/public/igem/BBa_K1907001/annotation2522063/range2522063/1
type: Range
persistent identity: range2522063
display id: range2522063
version: 1
direction: direction/forward
top level: MlrA
start: 1009
end: 1017
orientation: inline

MlrA CDS
type: SequenceAnnotation
persistent identity: annotation2522062
display id: annotation2522062
version: 1
top level: MlrA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1907001/annotation2522062/range2522062/1

https://synbiohub.org/public/igem/BBa_K1907001/annotation2522062/range2522062/1
type: Range
persistent identity: range2522062
display id: range2522062
version: 1
direction: direction/forward
top level: MlrA
start: 1
end: 1008
orientation: inline

BBa_K1125002
type: ComponentDefinition
persistent identity: BBa K1125002
display id: BBa_K1125002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tir1 promoter, Saccharomyces cerevisiae cold shock promoter
creator: Tsung-Yu, Lu
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1437_
m user id: 0
owner id: 17654
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: used to induce the expression of SrUCP under low temperature
mutable notes: presence of an invalid PstI site
mutable provenance: cloned from genomic DNA of Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1125002_sequence/1

BBa_K165091
type: ComponentDefinition
persistent identity: BBa K165091
display id: BBa_K165091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165011
component: BBa_K165018
component: BBa_K165031
component: BBa_K165006
component: BBa_K165004
component: BBa_K165003
component: BBa_K165002
sequence annotation: BBa_K165004
sequence annotation: BBa_K165018
sequence annotation: BBa_K165003
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence annotation: BBa_K165002
sequence annotation: BBa_K165006
sequence: https://synbiohub.org/public/igem/BBa_K165091_sequence/1

BBa_K165011
type: Component
persistent identity: component1997278
display id: component1997278
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165018
type: Component
persistent identity: component1997285
display id: component1997285
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165031
type: Component
persistent identity: component1997279
display id: component1997279
version: 1
top level: BBa_K165091
access: public
definition: BBa_K165031

BBa_K165006
type: Component
persistent identity: component1997282
display id: component1997282
version: 1
top level: BBa_K165091
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165004
type: Component
persistent identity: component1997284
display id: component1997284
version: 1
top level: BBa_K165091
access: public
definition: mCherry

BBa_K165003
type: Component
persistent identity: component1997281
display id: component1997281
version: 1
top level: BBa_K165091
access: public
definition: Sin3

BBa_K165002
type: Component
persistent identity: component1997280
display id: component1997280
version: 1
top level: BBa_K165091
access: public
definition: BBa_K165002

BBa_K165004
type: Component
persistent identity: component1997283
display id: component1997283
version: 1
top level: BBa_K165091
access: public
definition: mCherry

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997283
display id: annotation1997283
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997283/range1997283/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165091/annotation1997283/range1997283/1
type: Range
persistent identity: range1997283
display id: range1997283
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 3571
end: 4305
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997284
display id: annotation1997284
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997284/range1997284/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165091/annotation1997284/range1997284/1
type: Range
persistent identity: range1997284
display id: range1997284
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 4314
end: 5048
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997285
display id: annotation1997285
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997285/range1997285/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165091/annotation1997285/range1997285/1
type: Range
persistent identity: range1997285
display id: range1997285
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 5057
end: 5309
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997281
display id: annotation1997281
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997281/range1997281/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165091/annotation1997281/range1997281/1
type: Range
persistent identity: range1997281
display id: range1997281
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 492
end: 3302
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997279
display id: annotation1997279
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997279/range1997279/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165091/annotation1997279/range1997279/1
type: Range
persistent identity: range1997279
display id: range1997279
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997278
display id: annotation1997278
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997278/range1997278/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165091/annotation1997278/range1997278/1
type: Range
persistent identity: range1997278
display id: range1997278
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 1
end: 46
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997280
display id: annotation1997280
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997280/range1997280/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165091/annotation1997280/range1997280/1
type: Range
persistent identity: range1997280
display id: range1997280
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 466
end: 483
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997282
display id: annotation1997282
version: 1
top level: BBa_K165091
location: https://synbiohub.org/public/igem/BBa_K165091/annotation1997282/range1997282/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165091/annotation1997282/range1997282/1
type: Range
persistent identity: range1997282
display id: range1997282
version: 1
direction: direction/reverse
top level: BBa_K165091
start: 3311
end: 3562
orientation: inline

lexA
type: ComponentDefinition
persistent identity: BBa K165020
display id: BBa_K165020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA Synthetic Activator (untagged)
creator: John Szymanski
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a synthetic transcriptional activator for use in yeast. It contains the VP16 activation domain, the LexA DNA-binding domain, the SV40 nuclear localization sequence, and the ADH1 terminator. It binds to the Lex operator, activating transcription downstream.
mutable notes: -
mutable provenance: From David Drubin in Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K165020_sequence/1

BBa_K1907008
type: ComponentDefinition
persistent identity: BBa K1907008
display id: BBa_K1907008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCP1 promoter + Venus YFP
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T03:08:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1907005
component: BBa_K1907000
sequence annotation: BBa_K1907000
sequence annotation: BBa_K1907005
sequence: https://synbiohub.org/public/igem/BBa_K1907008_sequence/1

BBa_K1907005
type: Component
persistent identity: component2522670
display id: component2522670
version: 1
top level: BBa_K1907008
access: public
definition: BBa_K1907005

BBa_K1907000
type: Component
persistent identity: component2522671
display id: component2522671
version: 1
top level: BBa_K1907008
access: public
definition: Venus

BBa_K1907000
type: SequenceAnnotation
persistent identity: annotation2522671
display id: annotation2522671
version: 1
top level: BBa_K1907008
location: https://synbiohub.org/public/igem/BBa_K1907008/annotation2522671/range2522671/1
component: BBa_K1907000

https://synbiohub.org/public/igem/BBa_K1907008/annotation2522671/range2522671/1
type: Range
persistent identity: range2522671
display id: range2522671
version: 1
direction: direction/reverse
top level: BBa_K1907008
start: 763
end: 1479
orientation: inline

BBa_K1907005
type: SequenceAnnotation
persistent identity: annotation2522670
display id: annotation2522670
version: 1
top level: BBa_K1907008
location: https://synbiohub.org/public/igem/BBa_K1907008/annotation2522670/range2522670/1
component: BBa_K1907005

https://synbiohub.org/public/igem/BBa_K1907008/annotation2522670/range2522670/1
type: Range
persistent identity: range2522670
display id: range2522670
version: 1
direction: direction/reverse
top level: BBa_K1907008
start: 1
end: 756
orientation: inline

BBa_K1680013
type: ComponentDefinition
persistent identity: BBa K1680013
display id: BBa_K1680013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NDegron
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:42:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the firefly [Photinus pyralis] luciferase, codon optimized for yeast.
mutable notes: Shipped in RFC10
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NDegron
sequence annotation: SV40 NLS
sequence annotation: Spacer
sequence annotation: TEV cleavage sites
sequence annotation: Nuclear export sequence
sequence: https://synbiohub.org/public/igem/BBa_K1680013_sequence/1

NDegron
type: SequenceAnnotation
persistent identity: annotation2477347
display id: annotation2477347
version: 1
top level: BBa_K1680013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477347/range2477347/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477347/range2477347/1
type: Range
persistent identity: range2477347
display id: range2477347
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 1
end: 165
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477348
display id: annotation2477348
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477348/range2477348/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477348/range2477348/1
type: Range
persistent identity: range2477348
display id: range2477348
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 13
end: 33
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2477352
display id: annotation2477352
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477352/range2477352/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477352/range2477352/1
type: Range
persistent identity: range2477352
display id: range2477352
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 34
end: 66
orientation: inline

NDegron
type: SequenceAnnotation
persistent identity: annotation2477350
display id: annotation2477350
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477350/range2477350/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477350/range2477350/1
type: Range
persistent identity: range2477350
display id: range2477350
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 97
end: 138
orientation: inline

TEV cleavage sites
type: SequenceAnnotation
persistent identity: annotation2477349
display id: annotation2477349
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477349/range2477349/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477349/range2477349/1
type: Range
persistent identity: range2477349
display id: range2477349
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 67
end: 86
orientation: inline

Nuclear export sequence
type: SequenceAnnotation
persistent identity: annotation2477351
display id: annotation2477351
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477351/range2477351/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477351/range2477351/1
type: Range
persistent identity: range2477351
display id: range2477351
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 130
end: 147
orientation: inline

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Strep Tag
sequence annotation: Consensus sequence
sequence annotation: CaMXMT1
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1141
end: 1170
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1
end: 6
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 1140
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 9
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1171
end: 1176
orientation: inline

BBa_K110008
type: ComponentDefinition
persistent identity: BBa K110008
display id: BBa_K110008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: MCM1
sequence annotation: MATalpha2
sequence annotation: MFA1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K110008_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981090
display id: annotation1981090
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1
type: Range
persistent identity: range1981090
display id: range1981090
version: 1
direction: direction/forward
top level: BBa_K110008
start: 302
end: 309
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981088
display id: annotation1981088
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1
type: Range
persistent identity: range1981088
display id: range1981088
version: 1
direction: direction/forward
top level: BBa_K110008
start: 277
end: 287
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981089
display id: annotation1981089
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1
type: Range
persistent identity: range1981089
display id: range1981089
version: 1
direction: direction/forward
top level: BBa_K110008
start: 289
end: 297
orientation: inline

MFA1 Promoter
type: SequenceAnnotation
persistent identity: annotation1981086
display id: annotation1981086
version: 1
top level: BBa_K110008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1
type: Range
persistent identity: range1981086
display id: range1981086
version: 1
direction: direction/forward
top level: BBa_K110008
start: 1
end: 501
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981087
display id: annotation1981087
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1
type: Range
persistent identity: range1981087
display id: range1981087
version: 1
direction: direction/forward
top level: BBa_K110008
start: 267
end: 276
orientation: inline

BBa_K165035
type: ComponentDefinition
persistent identity: BBa K165035
display id: BBa_K165035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + Zif268-HIV bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165030
sequence annotation: BBa_K165012
sequence annotation: BBa_K165030
sequence: https://synbiohub.org/public/igem/BBa_K165035_sequence/1

BBa_K165012
type: Component
persistent identity: component1995495
display id: component1995495
version: 1
top level: BBa_K165035
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165030
type: Component
persistent identity: component1995497
display id: component1995497
version: 1
top level: BBa_K165035
access: public
definition: BBa_K165030

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995495
display id: annotation1995495
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
type: Range
persistent identity: range1995495
display id: range1995495
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 1
end: 127
orientation: inline

BBa_K165030
type: SequenceAnnotation
persistent identity: annotation1995497
display id: annotation1995497
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
component: BBa_K165030

https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
type: Range
persistent identity: range1995497
display id: range1995497
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 136
end: 442
orientation: inline

BBa_K416003
type: ComponentDefinition
persistent identity: BBa K416003
display id: BBa_K416003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Secretion Tag
creator: John Phair
created: 2010-06-22T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _527_
m user id: 0
owner id: 7430
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS.
mutable notes: There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
mutable provenance: This part was sent to use from Dr. Sheldon Park of the University of Buffalo.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K416003_sequence/1

BBa_K165086
type: ComponentDefinition
persistent identity: BBa K165086
display id: BBa_K165086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTEF2 + Untagged LexA activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165002
component: BBa_K165037
component: BBa_K165020
sequence annotation: BBa_K165002
sequence annotation: BBa_K165037
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165086_sequence/1

BBa_K165002
type: Component
persistent identity: component1997182
display id: component1997182
version: 1
top level: BBa_K165086
access: public
definition: BBa_K165002

BBa_K165037
type: Component
persistent identity: component1997181
display id: component1997181
version: 1
top level: BBa_K165086
access: public
definition: pTEF

BBa_K165020
type: Component
persistent identity: component1997183
display id: component1997183
version: 1
top level: BBa_K165086
access: public
definition: lexA

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997182
display id: annotation1997182
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997182/range1997182/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165086/annotation1997182/range1997182/1
type: Range
persistent identity: range1997182
display id: range1997182
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 412
end: 429
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997181
display id: annotation1997181
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997181/range1997181/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165086/annotation1997181/range1997181/1
type: Range
persistent identity: range1997181
display id: range1997181
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 1
end: 403
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997183
display id: annotation1997183
version: 1
top level: BBa_K165086
location: https://synbiohub.org/public/igem/BBa_K165086/annotation1997183/range1997183/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165086/annotation1997183/range1997183/1
type: Range
persistent identity: range1997183
display id: range1997183
version: 1
direction: direction/reverse
top level: BBa_K165086
start: 438
end: 1109
orientation: inline

BBa_K165095
type: ComponentDefinition
persistent identity: BBa K165095
display id: BBa_K165095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS303
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165004
component: BBa_K165006
component: BBa_K165018
component: BBa_K165002
component: BBa_K165031
component: BBa_K165003
component: BBa_K165012
sequence annotation: BBa_K165003
sequence annotation: BBa_K165031
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165004
sequence annotation: BBa_K165006
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165095_sequence/1

BBa_K165004
type: Component
persistent identity: component1997348
display id: component1997348
version: 1
top level: BBa_K165095
access: public
definition: mCherry

BBa_K165006
type: Component
persistent identity: component1997346
display id: component1997346
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165018
type: Component
persistent identity: component1997349
display id: component1997349
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165002
type: Component
persistent identity: component1997344
display id: component1997344
version: 1
top level: BBa_K165095
access: public
definition: BBa_K165002

BBa_K165031
type: Component
persistent identity: component1997343
display id: component1997343
version: 1
top level: BBa_K165095
access: public
definition: BBa_K165031

BBa_K165003
type: Component
persistent identity: component1997345
display id: component1997345
version: 1
top level: BBa_K165095
access: public
definition: Sin3

BBa_K165012
type: Component
persistent identity: component1997342
display id: component1997342
version: 1
top level: BBa_K165095
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165004
type: Component
persistent identity: component1997347
display id: component1997347
version: 1
top level: BBa_K165095
access: public
definition: mCherry

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997345
display id: annotation1997345
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997345/range1997345/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165095/annotation1997345/range1997345/1
type: Range
persistent identity: range1997345
display id: range1997345
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 573
end: 3383
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997343
display id: annotation1997343
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997343/range1997343/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165095/annotation1997343/range1997343/1
type: Range
persistent identity: range1997343
display id: range1997343
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 136
end: 538
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997349
display id: annotation1997349
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997349/range1997349/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165095/annotation1997349/range1997349/1
type: Range
persistent identity: range1997349
display id: range1997349
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 5138
end: 5390
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997344
display id: annotation1997344
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997344/range1997344/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165095/annotation1997344/range1997344/1
type: Range
persistent identity: range1997344
display id: range1997344
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 547
end: 564
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997347
display id: annotation1997347
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997347/range1997347/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165095/annotation1997347/range1997347/1
type: Range
persistent identity: range1997347
display id: range1997347
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 3652
end: 4386
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997346
display id: annotation1997346
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997346/range1997346/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165095/annotation1997346/range1997346/1
type: Range
persistent identity: range1997346
display id: range1997346
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 3392
end: 3643
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997348
display id: annotation1997348
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997348/range1997348/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165095/annotation1997348/range1997348/1
type: Range
persistent identity: range1997348
display id: range1997348
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 4395
end: 5129
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997342
display id: annotation1997342
version: 1
top level: BBa_K165095
location: https://synbiohub.org/public/igem/BBa_K165095/annotation1997342/range1997342/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165095/annotation1997342/range1997342/1
type: Range
persistent identity: range1997342
display id: range1997342
version: 1
direction: direction/reverse
top level: BBa_K165095
start: 1
end: 127
orientation: inline

CoxVI
type: ComponentDefinition
persistent identity: BBa K1462000
display id: BBa_K1462000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CoxVI
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: CoxVI is short for the subunit VI of the yeast cytochrome c oxidase, a N-terminal signal peptide to mitochondrial matrix.
mutable notes: 1
mutable provenance: CoxVI is obtained from Saccharomyces cerevisiae's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462000_sequence/1

BBa_K950001
type: ComponentDefinition
persistent identity: BBa K950001
display id: BBa_K950001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast rox1
creator: Jakob Matthes, Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: rox1 is a repressor. It targets the promotor anb1.
mutable notes: original sequence was not altered
mutable provenance: Sequence and information is based on the Saccharomyces Genome Database, see http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=rox1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rox1
sequence: https://synbiohub.org/public/igem/BBa_K950001_sequence/1

rox1
type: SequenceAnnotation
persistent identity: annotation2179266
display id: annotation2179266
version: 1
top level: BBa_K950001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950001/annotation2179266/range2179266/1

https://synbiohub.org/public/igem/BBa_K950001/annotation2179266/range2179266/1
type: Range
persistent identity: range2179266
display id: range2179266
version: 1
direction: direction/forward
top level: BBa_K950001
start: 1
end: 1107
orientation: inline

BBa_K1462060
type: ComponentDefinition
persistent identity: BBa K1462060
display id: BBa_K1462060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AdhE2
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462060_sequence/1

BBa_Y00100
type: ComponentDefinition
persistent identity: BBa Y00100
display id: BBa_Y00100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nuclear 5-aminolevulinate synthase gene from S. cerevisiae, recoded for mitochondrial expression
creator: Natalie Kuldell
created: 2006-08-03T11:00:00Z
modified: 2015-05-08T01:14:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _43_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The first step in S. cerevisiae's pathway for heme biosynthesis is catalyzed by the product of the HEM1 gene [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=HEM1]. HEM1 is a nuclear gene (1.647 kb,[http://db.yeastgenome.org/cgi-bin/getSeq?seq=YDR232W&flankl=0&flankr=0&map=a3map]) that encodes the enzyme 5-aminolevulinate ("ALA" or "d-ALA") synthase (549 aa,[http://db.yeastgenome.org/cgi-bin/getSeq?seq=YDR232W&flankl=0&flankr=0&map=p3map]). The enzyme is post-translationally imported into the mitochondria where it is responsible for converting glycine and succinyl-CoA to 5-aminolevulinate ("d-ALA") plus CoA and CO2. Deletion of HEM1 is lethal for the cell but viabilty can be restored by providing the cells with the product of the HEM1p catalyzed reaction, namely d-ALA. In an effort to directly express the HEM1 gene from the mitochondrial genome, the HEM1 gene was recoded by DNA 2.0 for mitochondrial codons, an epitope tag (HA = YPYDVPDYA) was added to the C-terminal portion of the protein just before the stop codon, and flanking sequences (100 bp upstm of ATG and 92 dwstm of stop) were added to facilitate integration into mitochondrial genome.
mutable notes: codon optimized for mitochondrial gene expression, HA-epitope C term (not N-term where mt import sequences rest), flank coding seq with homology to COX3 gene of mitochondria to allow for integration and disruption of this gene (should make respiration+ cells become respiration-), and finally added BB prefix and suffix to allow cloning into std Registry vectors
mutable provenance: direct synthesis from DNA 2.0, summer 2006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start codon
sequence annotation: TGA stop codon
sequence annotation: coding sequence
sequence annotation: for recombination to COX3
sequence annotation: HA
sequence: https://synbiohub.org/public/igem/BBa_Y00100_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1975605
display id: annotation1975605
version: 1
top level: BBa_Y00100
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975605/range1975605/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975605/range1975605/1
type: Range
persistent identity: range1975605
display id: range1975605
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 101
end: 103
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation1975603
display id: annotation1975603
version: 1
top level: BBa_Y00100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975603/range1975603/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975603/range1975603/1
type: Range
persistent identity: range1975603
display id: range1975603
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1772
end: 1774
orientation: inline

coding sequence
type: SequenceAnnotation
persistent identity: annotation1975606
display id: annotation1975606
version: 1
top level: BBa_Y00100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975606/range1975606/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975606/range1975606/1
type: Range
persistent identity: range1975606
display id: range1975606
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 101
end: 1774
orientation: inline

for recombination to COX3
type: SequenceAnnotation
persistent identity: annotation1975604
display id: annotation1975604
version: 1
top level: BBa_Y00100
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975604/range1975604/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975604/range1975604/1
type: Range
persistent identity: range1975604
display id: range1975604
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1775
end: 1865
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation1975602
display id: annotation1975602
version: 1
top level: BBa_Y00100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975602/range1975602/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975602/range1975602/1
type: Range
persistent identity: range1975602
display id: range1975602
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1745
end: 1771
orientation: inline

for recombination to COX3
type: SequenceAnnotation
persistent identity: annotation1975601
display id: annotation1975601
version: 1
top level: BBa_Y00100
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975601/range1975601/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975601/range1975601/1
type: Range
persistent identity: range1975601
display id: range1975601
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1
end: 101
orientation: inline

BBa_K165033
type: ComponentDefinition
persistent identity: BBa K165033
display id: BBa_K165033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 binding sites + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
mutable notes: -
mutable provenance: Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165013
component: BBa_K165016
sequence annotation: BBa_K165016
sequence annotation: BBa_K165013
sequence: https://synbiohub.org/public/igem/BBa_K165033_sequence/1

BBa_K165013
type: Component
persistent identity: component1995402
display id: component1995402
version: 1
top level: BBa_K165033
access: public
definition: https://synbiohub.org/public/igem/BBa_K165013/1

BBa_K165016
type: Component
persistent identity: component1995403
display id: component1995403
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165016

BBa_K165016
type: SequenceAnnotation
persistent identity: annotation1995403
display id: annotation1995403
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
component: BBa_K165016

https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
type: Range
persistent identity: range1995403
display id: range1995403
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 60
end: 304
orientation: inline

BBa_K165013
type: SequenceAnnotation
persistent identity: annotation1995402
display id: annotation1995402
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
component: BBa_K165013

https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
type: Range
persistent identity: range1995402
display id: range1995402
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 1
end: 51
orientation: inline

HisTag
type: ComponentDefinition
persistent identity: BBa K792005
display id: BBa_K792005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine rich peptide (His Tag)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble Histidine rich peptide.
mutable notes: Retro-translated and optimized (codons and mRNA seconday structure) for yeast.
mutable provenance: Clontech???s His-tag.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HindIII restriction site
sequence annotation: stop
sequence annotation: His tag
sequence: https://synbiohub.org/public/igem/BBa_K792005_sequence/1

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196426
display id: annotation2196426
version: 1
top level: HisTag
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2196426/range2196426/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2196426/range2196426/1
type: Range
persistent identity: range2196426
display id: range2196426
version: 1
direction: direction/forward
top level: HisTag
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2196557
display id: annotation2196557
version: 1
top level: HisTag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2196557/range2196557/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2196557/range2196557/1
type: Range
persistent identity: range2196557
display id: range2196557
version: 1
direction: direction/forward
top level: HisTag
start: 43
end: 48
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2197882
display id: annotation2197882
version: 1
top level: HisTag
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2197882/range2197882/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2197882/range2197882/1
type: Range
persistent identity: range2197882
display id: range2197882
version: 1
direction: direction/forward
top level: HisTag
start: 7
end: 42
orientation: inline

BBa_K165092
type: ComponentDefinition
persistent identity: BBa K165092
display id: BBa_K165092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Gli1 repressor (mCherryx2 tagged) on pRS303
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165004
component: BBa_K165018
component: BBa_K165003
component: BBa_K165031
component: BBa_K165002
component: BBa_K165007
component: BBa_K165011
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165007
sequence annotation: BBa_K165004
sequence annotation: BBa_K165011
sequence annotation: BBa_K165018
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165092_sequence/1

BBa_K165004
type: Component
persistent identity: component1997317
display id: component1997317
version: 1
top level: BBa_K165092
access: public
definition: mCherry

BBa_K165018
type: Component
persistent identity: component1997318
display id: component1997318
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165003
type: Component
persistent identity: component1997314
display id: component1997314
version: 1
top level: BBa_K165092
access: public
definition: Sin3

BBa_K165031
type: Component
persistent identity: component1997312
display id: component1997312
version: 1
top level: BBa_K165092
access: public
definition: BBa_K165031

BBa_K165002
type: Component
persistent identity: component1997313
display id: component1997313
version: 1
top level: BBa_K165092
access: public
definition: BBa_K165002

BBa_K165007
type: Component
persistent identity: component1997315
display id: component1997315
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165004
type: Component
persistent identity: component1997316
display id: component1997316
version: 1
top level: BBa_K165092
access: public
definition: mCherry

BBa_K165011
type: Component
persistent identity: component1997311
display id: component1997311
version: 1
top level: BBa_K165092
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997313
display id: annotation1997313
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997313/range1997313/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165092/annotation1997313/range1997313/1
type: Range
persistent identity: range1997313
display id: range1997313
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 466
end: 483
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997314
display id: annotation1997314
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997314/range1997314/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165092/annotation1997314/range1997314/1
type: Range
persistent identity: range1997314
display id: range1997314
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 492
end: 3302
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997315
display id: annotation1997315
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997315/range1997315/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165092/annotation1997315/range1997315/1
type: Range
persistent identity: range1997315
display id: range1997315
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 3311
end: 3835
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997317
display id: annotation1997317
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997317/range1997317/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165092/annotation1997317/range1997317/1
type: Range
persistent identity: range1997317
display id: range1997317
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 4587
end: 5321
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997311
display id: annotation1997311
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997311/range1997311/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165092/annotation1997311/range1997311/1
type: Range
persistent identity: range1997311
display id: range1997311
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 1
end: 46
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997318
display id: annotation1997318
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997318/range1997318/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165092/annotation1997318/range1997318/1
type: Range
persistent identity: range1997318
display id: range1997318
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 5330
end: 5582
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997316
display id: annotation1997316
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997316/range1997316/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165092/annotation1997316/range1997316/1
type: Range
persistent identity: range1997316
display id: range1997316
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 3844
end: 4578
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997312
display id: annotation1997312
version: 1
top level: BBa_K165092
location: https://synbiohub.org/public/igem/BBa_K165092/annotation1997312/range1997312/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165092/annotation1997312/range1997312/1
type: Range
persistent identity: range1997312
display id: range1997312
version: 1
direction: direction/reverse
top level: BBa_K165092
start: 55
end: 457
orientation: inline

BBa_K165059
type: ComponentDefinition
persistent identity: BBa K165059
display id: BBa_K165059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak + CFPx2
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak + CFPx2
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K165002
component: BBa_K165014
sequence annotation: BBa_K165014
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165059_sequence/1

BBa_K165002
type: Component
persistent identity: component1997050
display id: component1997050
version: 1
top level: BBa_K165059
access: public
definition: BBa_K165002

BBa_K165014
type: Component
persistent identity: component1997051
display id: component1997051
version: 1
top level: BBa_K165059
access: public
definition: cfpx2

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997051
display id: annotation1997051
version: 1
top level: BBa_K165059
location: https://synbiohub.org/public/igem/BBa_K165059/annotation1997051/range1997051/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165059/annotation1997051/range1997051/1
type: Range
persistent identity: range1997051
display id: range1997051
version: 1
direction: direction/reverse
top level: BBa_K165059
start: 27
end: 1520
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997050
display id: annotation1997050
version: 1
top level: BBa_K165059
location: https://synbiohub.org/public/igem/BBa_K165059/annotation1997050/range1997050/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165059/annotation1997050/range1997050/1
type: Range
persistent identity: range1997050
display id: range1997050
version: 1
direction: direction/reverse
top level: BBa_K165059
start: 1
end: 18
orientation: inline

BBa_K1728024
type: ComponentDefinition
persistent identity: BBa K1728024
display id: BBa_K1728024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhodopsin siganl peptide +IL-8 receptor type alpha (CXCR1)
creator: Wan-Yun Chiu, Timothy Chan En Haw, Ming-Fei Hsieh
created: 2015-09-16T11:00:00Z
modified: 2016-06-18T03:40:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2149_
m user id: 18379
owner id: 18379
owning group id: 9
status: status/Planning
bookmark: true
mutable description: rhodopsin+Chemokine receptor 1 (CXCR1)
mutable notes: No.
mutable provenance: Order the DNA part from synthesis company
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1728020
component: BBa_J18920
component: BBa_K1728021
sequence annotation: BBa_K1728020
sequence annotation: BBa_J18920
sequence annotation: BBa_K1728021
sequence: https://synbiohub.org/public/igem/BBa_K1728024_sequence/1

BBa_K1728020
type: Component
persistent identity: component2473265
display id: component2473265
version: 1
top level: BBa_K1728024
access: public
definition: BBa_K1728020

BBa_J18920
type: Component
persistent identity: component2473266
display id: component2473266
version: 1
top level: BBa_K1728024
access: public
definition: https://synbiohub.org/public/igem/BBa_J18920/1

BBa_K1728021
type: Component
persistent identity: component2473267
display id: component2473267
version: 1
top level: BBa_K1728024
access: public
definition: BBa_K1728021

BBa_K1728020
type: SequenceAnnotation
persistent identity: annotation2473265
display id: annotation2473265
version: 1
top level: BBa_K1728024
location: https://synbiohub.org/public/igem/BBa_K1728024/annotation2473265/range2473265/1
component: BBa_K1728020

https://synbiohub.org/public/igem/BBa_K1728024/annotation2473265/range2473265/1
type: Range
persistent identity: range2473265
display id: range2473265
version: 1
direction: direction/reverse
top level: BBa_K1728024
start: 1
end: 60
orientation: inline

BBa_J18920
type: SequenceAnnotation
persistent identity: annotation2473266
display id: annotation2473266
version: 1
top level: BBa_K1728024
location: https://synbiohub.org/public/igem/BBa_K1728024/annotation2473266/range2473266/1
component: BBa_J18920

https://synbiohub.org/public/igem/BBa_K1728024/annotation2473266/range2473266/1
type: Range
persistent identity: range2473266
display id: range2473266
version: 1
direction: direction/reverse
top level: BBa_K1728024
start: 61
end: 66
orientation: inline

BBa_K1728021
type: SequenceAnnotation
persistent identity: annotation2473267
display id: annotation2473267
version: 1
top level: BBa_K1728024
location: https://synbiohub.org/public/igem/BBa_K1728024/annotation2473267/range2473267/1
component: BBa_K1728021

https://synbiohub.org/public/igem/BBa_K1728024/annotation2473267/range2473267/1
type: Range
persistent identity: range2473267
display id: range2473267
version: 1
direction: direction/reverse
top level: BBa_K1728024
start: 67
end: 1119
orientation: inline

BBa_K801075
type: ComponentDefinition
persistent identity: BBa K801075
display id: BBa_K801075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaDXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Yeast expression cassette for CaDXMT1 (BBa_K801072) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012)
mutable notes: -
mutable provenance: Composite part of BBa_K801010, BBa_K801072 and BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Cassette
sequence annotation: Strep-Tag
sequence annotation: Consensus sequence
sequence annotation: ADH1 Terminator
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: CaDXMT1
sequence annotation: Tef2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K801075_sequence/1

Cassette
type: SequenceAnnotation
persistent identity: annotation2189809
display id: annotation2189809
version: 1
top level: BBa_K801075
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2189809/range2189809/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2189809/range2189809/1
type: Range
persistent identity: range2189809
display id: range2189809
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1
end: 2208
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199509
display id: annotation2199509
version: 1
top level: BBa_K801075
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199509/range2199509/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199509/range2199509/1
type: Range
persistent identity: range2199509
display id: range2199509
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1815
end: 1844
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199506
display id: annotation2199506
version: 1
top level: BBa_K801075
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199506/range2199506/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199506/range2199506/1
type: Range
persistent identity: range2199506
display id: range2199506
version: 1
direction: direction/forward
top level: BBa_K801075
start: 657
end: 662
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197243
display id: annotation2197243
version: 1
top level: BBa_K801075
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197243/range2197243/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197243/range2197243/1
type: Range
persistent identity: range2197243
display id: range2197243
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1859
end: 2208
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201347
display id: annotation2201347
version: 1
top level: BBa_K801075
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2201347/range2201347/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2201347/range2201347/1
type: Range
persistent identity: range2201347
display id: range2201347
version: 1
direction: direction/forward
top level: BBa_K801075
start: 663
end: 665
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199508
display id: annotation2199508
version: 1
top level: BBa_K801075
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199508/range2199508/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199508/range2199508/1
type: Range
persistent identity: range2199508
display id: range2199508
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1845
end: 1850
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197242
display id: annotation2197242
version: 1
top level: BBa_K801075
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197242/range2197242/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197242/range2197242/1
type: Range
persistent identity: range2197242
display id: range2197242
version: 1
direction: direction/forward
top level: BBa_K801075
start: 663
end: 1814
orientation: inline

Tef2 promoter
type: SequenceAnnotation
persistent identity: annotation2197241
display id: annotation2197241
version: 1
top level: BBa_K801075
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197241/range2197241/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197241/range2197241/1
type: Range
persistent identity: range2197241
display id: range2197241
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1
end: 648
orientation: inline

TAT-trojan
type: ComponentDefinition
persistent identity: BBa K792003
display id: BBa_K792003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Import enhancer - HIV TAT penetratin (trojan peptide)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell. This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
mutable notes: Sequence obtained by retro-translation
mutable provenance: HIV TAT penetratin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: HindIII restriction site
sequence annotation: Trojan peptide
sequence: https://synbiohub.org/public/igem/BBa_K792003_sequence/1

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196325
display id: annotation2196325
version: 1
top level: TAT-trojan
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792003/annotation2196325/range2196325/1

https://synbiohub.org/public/igem/BBa_K792003/annotation2196325/range2196325/1
type: Range
persistent identity: range2196325
display id: range2196325
version: 1
direction: direction/forward
top level: TAT-trojan
start: 1
end: 6
orientation: inline

Trojan peptide
type: SequenceAnnotation
persistent identity: annotation2197881
display id: annotation2197881
version: 1
top level: TAT-trojan
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792003/annotation2197881/range2197881/1

https://synbiohub.org/public/igem/BBa_K792003/annotation2197881/range2197881/1
type: Range
persistent identity: range2197881
display id: range2197881
version: 1
direction: direction/forward
top level: TAT-trojan
start: 7
end: 39
orientation: inline

BBa_K124000
type: ComponentDefinition
persistent identity: BBa K124000
display id: BBa_K124000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCYC Yeast Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
mutable notes: none
mutable provenance: From Mumberg, Muller, and Funk p413 pCYC. See ref. above
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124000_sequence/1

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165037
component: BBa_K165011
sequence annotation: BBa_K165037
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: pTEF

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 1
end: 46
orientation: inline

BBa_K801094
type: ComponentDefinition
persistent identity: BBa K801094
display id: BBa_K801094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Naringenin - chalcone synthase(CHS) coding sequence from ''Hypericum androsaemum'' preceeded by yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/11096318/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: CHS
sequence: https://synbiohub.org/public/igem/BBa_K801094_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2202149
display id: annotation2202149
version: 1
top level: BBa_K801094
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202149/range2202149/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202149/range2202149/1
type: Range
persistent identity: range2202149
display id: range2202149
version: 1
direction: direction/forward
top level: BBa_K801094
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202150
display id: annotation2202150
version: 1
top level: BBa_K801094
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202150/range2202150/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202150/range2202150/1
type: Range
persistent identity: range2202150
display id: range2202150
version: 1
direction: direction/forward
top level: BBa_K801094
start: 7
end: 9
orientation: inline

CHS
type: SequenceAnnotation
persistent identity: annotation2202151
display id: annotation2202151
version: 1
top level: BBa_K801094
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202151/range2202151/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202151/range2202151/1
type: Range
persistent identity: range2202151
display id: range2202151
version: 1
direction: direction/forward
top level: BBa_K801094
start: 1
end: 1176
orientation: inline

BBa_K110006
type: ComponentDefinition
persistent identity: BBa K110006
display id: BBa_K110006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)1 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)1
sequence: https://synbiohub.org/public/igem/BBa_K110006_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997690
display id: annotation1997690
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1
type: Range
persistent identity: range1997690
display id: range1997690
version: 1
direction: direction/forward
top level: BBa_K110006
start: 92
end: 102
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997688
display id: annotation1997688
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1
type: Range
persistent identity: range1997688
display id: range1997688
version: 1
direction: direction/forward
top level: BBa_K110006
start: 19
end: 34
orientation: inline

MF(alpha)1
type: SequenceAnnotation
persistent identity: annotation1997687
display id: annotation1997687
version: 1
top level: BBa_K110006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1
type: Range
persistent identity: range1997687
display id: range1997687
version: 1
direction: direction/forward
top level: BBa_K110006
start: 1
end: 501
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997689
display id: annotation1997689
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1
type: Range
persistent identity: range1997689
display id: range1997689
version: 1
direction: direction/forward
top level: BBa_K110006
start: 136
end: 161
orientation: inline

BBa_E2020
type: ComponentDefinition
persistent identity: BBa E2020
display id: BBa_E2020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of ECFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP cerulean, yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Venus YFP (BBa_E2030) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: Rizzo MA, et al. (2004) Nat Biot (22):445-9
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: MATSG linker
sequence annotation: Help:Barcodes
sequence annotation: GSGTA linker
sequence annotation: Cerulean CFP
sequence: https://synbiohub.org/public/igem/BBa_E2020_sequence/1

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431972
display id: annotation1431972
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1
type: Range
persistent identity: range1431972
display id: range1431972
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 15
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214038
display id: annotation2214038
version: 1
top level: BBa_E2020
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1

https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1
type: Range
persistent identity: range2214038
display id: range2214038
version: 1
direction: direction/forward
top level: BBa_E2020
start: 754
end: 778
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431973
display id: annotation1431973
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1
type: Range
persistent identity: range1431973
display id: range1431973
version: 1
direction: direction/forward
top level: BBa_E2020
start: 733
end: 747
orientation: inline

Cerulean CFP
type: SequenceAnnotation
persistent identity: annotation1431974
display id: annotation1431974
version: 1
top level: BBa_E2020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1
type: Range
persistent identity: range1431974
display id: range1431974
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 747
orientation: inline

BBa_K775000
type: ComponentDefinition
persistent identity: BBa K775000
display id: BBa_K775000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-GPR109A Niacin chimeric GPCR
creator: Sietske Grijseels
created: 2012-08-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: Promoter: GPD Terminator: CYC1.
mutable provenance: Homo sapiens NP_808219.1 Rat
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CYC1 terminator
sequence annotation: Startcodon
sequence annotation: BamHI restriction site
sequence annotation: C terminus RI7
sequence annotation: FLAGtag
sequence annotation: NdeI restriction site
sequence annotation: N terminus RI7
sequence annotation: GPD promoter
sequence annotation: Niacin receptor part
sequence annotation: Kozak sequence
sequence annotation: Stopcodon
sequence: https://synbiohub.org/public/igem/BBa_K775000_sequence/1

CYC1 terminator
type: SequenceAnnotation
persistent identity: annotation2181646
display id: annotation2181646
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181646/range2181646/1
type: Range
persistent identity: range2181646
display id: range2181646
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1163
end: 1414
orientation: inline

Startcodon
type: SequenceAnnotation
persistent identity: annotation2181641
display id: annotation2181641
version: 1
top level: BBa_K775000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181641/range2181641/1
type: Range
persistent identity: range2181641
display id: range2181641
version: 1
direction: direction/forward
top level: BBa_K775000
start: 125
end: 127
orientation: inline

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation2181639
display id: annotation2181639
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181639/range2181639/1
type: Range
persistent identity: range2181639
display id: range2181639
version: 1
direction: direction/forward
top level: BBa_K775000
start: 113
end: 118
orientation: inline

C terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181647
display id: annotation2181647
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181647/range2181647/1
type: Range
persistent identity: range2181647
display id: range2181647
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1055
end: 1150
orientation: inline

FLAGtag
type: SequenceAnnotation
persistent identity: annotation2181642
display id: annotation2181642
version: 1
top level: BBa_K775000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181642/range2181642/1
type: Range
persistent identity: range2181642
display id: range2181642
version: 1
direction: direction/forward
top level: BBa_K775000
start: 128
end: 151
orientation: inline

NdeI restriction site
type: SequenceAnnotation
persistent identity: annotation2181645
display id: annotation2181645
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181645/range2181645/1
type: Range
persistent identity: range2181645
display id: range2181645
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1157
end: 1162
orientation: inline

N terminus RI7
type: SequenceAnnotation
persistent identity: annotation2181643
display id: annotation2181643
version: 1
top level: BBa_K775000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181643/range2181643/1
type: Range
persistent identity: range2181643
display id: range2181643
version: 1
direction: direction/forward
top level: BBa_K775000
start: 152
end: 337
orientation: inline

GPD promoter
type: SequenceAnnotation
persistent identity: annotation2181638
display id: annotation2181638
version: 1
top level: BBa_K775000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181638/range2181638/1
type: Range
persistent identity: range2181638
display id: range2181638
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1
end: 112
orientation: inline

Niacin receptor part
type: SequenceAnnotation
persistent identity: annotation2181648
display id: annotation2181648
version: 1
top level: BBa_K775000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181648/range2181648/1
type: Range
persistent identity: range2181648
display id: range2181648
version: 1
direction: direction/forward
top level: BBa_K775000
start: 338
end: 1054
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2181640
display id: annotation2181640
version: 1
top level: BBa_K775000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181640/range2181640/1
type: Range
persistent identity: range2181640
display id: range2181640
version: 1
direction: direction/forward
top level: BBa_K775000
start: 119
end: 124
orientation: inline

Stopcodon
type: SequenceAnnotation
persistent identity: annotation2181644
display id: annotation2181644
version: 1
top level: BBa_K775000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1

https://synbiohub.org/public/igem/BBa_K775000/annotation2181644/range2181644/1
type: Range
persistent identity: range2181644
display id: range2181644
version: 1
direction: direction/forward
top level: BBa_K775000
start: 1151
end: 1156
orientation: inline

BBa_K792011
type: ComponentDefinition
persistent identity: BBa K792011
display id: BBa_K792011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast exportable His-rich peptide w/enhanced import (2)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pato
mutable notes: Composite device
mutable provenance: Composite device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K416003
component: BBa_J63003
component: BBa_K792004
component: BBa_K792007
component: BBa_K792013
sequence annotation: BBa_K416003
sequence annotation: BBa_K792013
sequence annotation: BBa_K792004
sequence annotation: BBa_J63003
sequence annotation: BBa_K792007
sequence: https://synbiohub.org/public/igem/BBa_K792011_sequence/1

BBa_K416003
type: Component
persistent identity: component2197302
display id: component2197302
version: 1
top level: BBa_K792011
access: public
definition: BBa_K416003

BBa_J63003
type: Component
persistent identity: component2197301
display id: component2197301
version: 1
top level: BBa_K792011
access: public
definition: Kozak & st

BBa_K792004
type: Component
persistent identity: component2197304
display id: component2197304
version: 1
top level: BBa_K792011
access: public
definition: Polyargini

BBa_K792007
type: Component
persistent identity: component2197307
display id: component2197307
version: 1
top level: BBa_K792011
access: public
definition: PolyHb

BBa_K792013
type: Component
persistent identity: component2197299
display id: component2197299
version: 1
top level: BBa_K792011
access: public
definition: https://synbiohub.org/public/igem/BBa_K792013/1

BBa_K416003
type: SequenceAnnotation
persistent identity: annotation2197302
display id: annotation2197302
version: 1
top level: BBa_K792011
location: https://synbiohub.org/public/igem/BBa_K792011/annotation2197302/range2197302/1
component: BBa_K416003

https://synbiohub.org/public/igem/BBa_K792011/annotation2197302/range2197302/1
type: Range
persistent identity: range2197302
display id: range2197302
version: 1
direction: direction/reverse
top level: BBa_K792011
start: 25
end: 138
orientation: inline

BBa_K792013
type: SequenceAnnotation
persistent identity: annotation2197299
display id: annotation2197299
version: 1
top level: BBa_K792011
location: https://synbiohub.org/public/igem/BBa_K792011/annotation2197299/range2197299/1
component: BBa_K792013

https://synbiohub.org/public/igem/BBa_K792011/annotation2197299/range2197299/1
type: Range
persistent identity: range2197299
display id: range2197299
version: 1
direction: direction/reverse
top level: BBa_K792011
start: 1
end: 6
orientation: inline

BBa_K792004
type: SequenceAnnotation
persistent identity: annotation2197304
display id: annotation2197304
version: 1
top level: BBa_K792011
location: https://synbiohub.org/public/igem/BBa_K792011/annotation2197304/range2197304/1
component: BBa_K792004

https://synbiohub.org/public/igem/BBa_K792011/annotation2197304/range2197304/1
type: Range
persistent identity: range2197304
display id: range2197304
version: 1
direction: direction/reverse
top level: BBa_K792011
start: 139
end: 177
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2197301
display id: annotation2197301
version: 1
top level: BBa_K792011
location: https://synbiohub.org/public/igem/BBa_K792011/annotation2197301/range2197301/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_K792011/annotation2197301/range2197301/1
type: Range
persistent identity: range2197301
display id: range2197301
version: 1
direction: direction/reverse
top level: BBa_K792011
start: 7
end: 24
orientation: inline

BBa_K792007
type: SequenceAnnotation
persistent identity: annotation2197307
display id: annotation2197307
version: 1
top level: BBa_K792011
location: https://synbiohub.org/public/igem/BBa_K792011/annotation2197307/range2197307/1
component: BBa_K792007

https://synbiohub.org/public/igem/BBa_K792011/annotation2197307/range2197307/1
type: Range
persistent identity: range2197307
display id: range2197307
version: 1
direction: direction/reverse
top level: BBa_K792011
start: 178
end: 297
orientation: inline

BBa_K110009
type: ComponentDefinition
persistent identity: BBa K110009
display id: BBa_K110009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This promotor is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mat Alpha2
sequence annotation: MCM1
sequence annotation: Ste2 Promoter
sequence annotation: STE12
sequence: https://synbiohub.org/public/igem/BBa_K110009_sequence/1

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981499
display id: annotation1981499
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1
type: Range
persistent identity: range1981499
display id: range1981499
version: 1
direction: direction/forward
top level: BBa_K110009
start: 273
end: 280
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981500
display id: annotation1981500
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1
type: Range
persistent identity: range1981500
display id: range1981500
version: 1
direction: direction/forward
top level: BBa_K110009
start: 283
end: 292
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981501
display id: annotation1981501
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1
type: Range
persistent identity: range1981501
display id: range1981501
version: 1
direction: direction/forward
top level: BBa_K110009
start: 295
end: 301
orientation: inline

Ste2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981498
display id: annotation1981498
version: 1
top level: BBa_K110009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1
type: Range
persistent identity: range1981498
display id: range1981498
version: 1
direction: direction/forward
top level: BBa_K110009
start: 1
end: 501
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981502
display id: annotation1981502
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1
type: Range
persistent identity: range1981502
display id: range1981502
version: 1
direction: direction/forward
top level: BBa_K110009
start: 304
end: 312
orientation: inline

BBa_K165097
type: ComponentDefinition
persistent identity: BBa K165097
display id: BBa_K165097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + MET25+ LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165000
component: BBa_K165009
component: BBa_K165003
component: BBa_K165002
component: BBa_K165012
component: BBa_K165014
component: BBa_K165018
sequence annotation: BBa_K165003
sequence annotation: BBa_K165002
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence annotation: BBa_K165018
sequence annotation: BBa_K165009
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165097_sequence/1

BBa_K165000
type: Component
persistent identity: component1997368
display id: component1997368
version: 1
top level: BBa_K165097
access: public
definition: BBa_K165000

BBa_K165009
type: Component
persistent identity: component1997370
display id: component1997370
version: 1
top level: BBa_K165097
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165003
type: Component
persistent identity: component1997371
display id: component1997371
version: 1
top level: BBa_K165097
access: public
definition: Sin3

BBa_K165002
type: Component
persistent identity: component1997369
display id: component1997369
version: 1
top level: BBa_K165097
access: public
definition: BBa_K165002

BBa_K165012
type: Component
persistent identity: component1997367
display id: component1997367
version: 1
top level: BBa_K165097
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165014
type: Component
persistent identity: component1997372
display id: component1997372
version: 1
top level: BBa_K165097
access: public
definition: cfpx2

BBa_K165018
type: Component
persistent identity: component1997373
display id: component1997373
version: 1
top level: BBa_K165097
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997371
display id: annotation1997371
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997371/range1997371/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165097/annotation1997371/range1997371/1
type: Range
persistent identity: range1997371
display id: range1997371
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 823
end: 3633
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997369
display id: annotation1997369
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997369/range1997369/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165097/annotation1997369/range1997369/1
type: Range
persistent identity: range1997369
display id: range1997369
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 531
end: 548
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997367
display id: annotation1997367
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997367/range1997367/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165097/annotation1997367/range1997367/1
type: Range
persistent identity: range1997367
display id: range1997367
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 1
end: 127
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997368
display id: annotation1997368
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997368/range1997368/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165097/annotation1997368/range1997368/1
type: Range
persistent identity: range1997368
display id: range1997368
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 136
end: 522
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997373
display id: annotation1997373
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997373/range1997373/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165097/annotation1997373/range1997373/1
type: Range
persistent identity: range1997373
display id: range1997373
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 5144
end: 5396
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997370
display id: annotation1997370
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997370/range1997370/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165097/annotation1997370/range1997370/1
type: Range
persistent identity: range1997370
display id: range1997370
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 557
end: 814
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997372
display id: annotation1997372
version: 1
top level: BBa_K165097
location: https://synbiohub.org/public/igem/BBa_K165097/annotation1997372/range1997372/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165097/annotation1997372/range1997372/1
type: Range
persistent identity: range1997372
display id: range1997372
version: 1
direction: direction/reverse
top level: BBa_K165097
start: 3642
end: 5135
orientation: inline

BBa_K165039
type: ComponentDefinition
persistent identity: BBa K165039
display id: BBa_K165039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + ADH1 yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165011
sequence annotation: BBa_K165011
sequence annotation: BBa_K165015
sequence: https://synbiohub.org/public/igem/BBa_K165039_sequence/1

BBa_K165015
type: Component
persistent identity: component1995517
display id: component1995517
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165015

BBa_K165011
type: Component
persistent identity: component1995516
display id: component1995516
version: 1
top level: BBa_K165039
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995516
display id: annotation1995516
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
type: Range
persistent identity: range1995516
display id: range1995516
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 1
end: 46
orientation: inline

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995517
display id: annotation1995517
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
type: Range
persistent identity: range1995517
display id: range1995517
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 55
end: 1499
orientation: inline

BBa_K950004
type: ComponentDefinition
persistent identity: BBa K950004
display id: BBa_K950004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: firefly luciferase
creator: Jakob Matthes
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a optimized firefly luciferase (C-terminal modification, see design notes).
mutable notes: Primers were designed to replace the C-terminal SKL aminoacids with KL.
mutable provenance: The original source is the Firefly luciferase, see http://www.ncbi.nlm.nih.gov/nuccore/AB644228.1 The modification is covered by 'One-step measurement of firefly luciferase activity in yeast' (http://www.ncbi.nlm.nih.gov/pubmed/14558144).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: luciferase
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_K950004_sequence/1

luciferase
type: SequenceAnnotation
persistent identity: annotation2182744
display id: annotation2182744
version: 1
top level: BBa_K950004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950004/annotation2182744/range2182744/1

https://synbiohub.org/public/igem/BBa_K950004/annotation2182744/range2182744/1
type: Range
persistent identity: range2182744
display id: range2182744
version: 1
direction: direction/forward
top level: BBa_K950004
start: 1
end: 1650
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2182745
display id: annotation2182745
version: 1
top level: BBa_K950004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950004/annotation2182745/range2182745/1

https://synbiohub.org/public/igem/BBa_K950004/annotation2182745/range2182745/1
type: Range
persistent identity: range2182745
display id: range2182745
version: 1
direction: direction/forward
top level: BBa_K950004
start: 48
end: 54
orientation: inline

BBa_K1680014
type: ComponentDefinition
persistent identity: BBa K1680014
display id: BBa_K1680014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:47:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampR promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: ampR
sequence annotation: pUC origin
sequence annotation: T3 promotor
sequence annotation: RFC10 MCS
sequence annotation: f1 ori
sequence annotation: T7 promotor
sequence annotation: HIS3 promotor
sequence annotation: lacZalpha
sequence annotation: lac promotor
sequence annotation: HIS3
sequence: https://synbiohub.org/public/igem/BBa_K1680014_sequence/1

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466130
display id: annotation2466130
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1
type: Range
persistent identity: range2466130
display id: range2466130
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 4215
end: 4319
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466131
display id: annotation2466131
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1
type: Range
persistent identity: range2466131
display id: range2466131
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 4356
end: 4859
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466129
display id: annotation2466129
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1
type: Range
persistent identity: range2466129
display id: range2466129
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 3354
end: 4214
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466128
display id: annotation2466128
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1
type: Range
persistent identity: range2466128
display id: range2466128
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2595
end: 3183
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466125
display id: annotation2466125
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1
type: Range
persistent identity: range2466125
display id: range2466125
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2153
end: 2172
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466135
display id: annotation2466135
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1
type: Range
persistent identity: range2466135
display id: range2466135
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2083
end: 2125
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2466099
display id: annotation2466099
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1
type: Range
persistent identity: range2466099
display id: range2466099
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 1423
end: 1878
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466133
display id: annotation2466133
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1
type: Range
persistent identity: range2466133
display id: range2466133
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2046
end: 2065
orientation: inline

HIS3 promotor
type: SequenceAnnotation
persistent identity: annotation2466090
display id: annotation2466090
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1
type: Range
persistent identity: range2466090
display id: range2466090
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 315
end: 502
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466100
display id: annotation2466100
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1
type: Range
persistent identity: range2466100
display id: range2466100
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 1661
end: 2077
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466127
display id: annotation2466127
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1
type: Range
persistent identity: range2466127
display id: range2466127
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2241
end: 2271
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2466098
display id: annotation2466098
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1
type: Range
persistent identity: range2466098
display id: range2466098
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 503
end: 1162
orientation: inline

BBa_K165040
type: ComponentDefinition
persistent identity: BBa K165040
display id: BBa_K165040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165037
sequence annotation: BBa_K165012
sequence annotation: BBa_K165037
sequence: https://synbiohub.org/public/igem/BBa_K165040_sequence/1

BBa_K165012
type: Component
persistent identity: component1996555
display id: component1996555
version: 1
top level: BBa_K165040
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165037
type: Component
persistent identity: component1996556
display id: component1996556
version: 1
top level: BBa_K165040
access: public
definition: pTEF

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996555
display id: annotation1996555
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
type: Range
persistent identity: range1996555
display id: range1996555
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 1
end: 127
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996556
display id: annotation1996556
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
type: Range
persistent identity: range1996556
display id: range1996556
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 136
end: 538
orientation: inline

BBa_K1462070
type: ComponentDefinition
persistent identity: BBa K1462070
display id: BBa_K1462070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc1
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1462070_sequence/1

BBa_K801011
type: ComponentDefinition
persistent identity: BBa K801011
display id: BBa_K801011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TEF1 terminator of ''S. cervisiae''.
mutable notes: Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TEF1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801011_sequence/1

TEF1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202142
display id: annotation2202142
version: 1
top level: BBa_K801011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1

https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
type: Range
persistent identity: range2202142
display id: range2202142
version: 1
direction: direction/forward
top level: BBa_K801011
start: 1
end: 507
orientation: inline

BBa_K165101
type: ComponentDefinition
persistent identity: BBa K165101
display id: BBa_K165101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165018
component: BBa_K165004
component: BBa_K165002
component: BBa_K165031
component: BBa_K165003
component: BBa_K165011
component: BBa_K165006
sequence annotation: BBa_K165006
sequence annotation: BBa_K165004
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165101_sequence/1

BBa_K165018
type: Component
persistent identity: component1997412
display id: component1997412
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1997411
display id: component1997411
version: 1
top level: BBa_K165101
access: public
definition: mCherry

BBa_K165002
type: Component
persistent identity: component1997407
display id: component1997407
version: 1
top level: BBa_K165101
access: public
definition: BBa_K165002

BBa_K165031
type: Component
persistent identity: component1997406
display id: component1997406
version: 1
top level: BBa_K165101
access: public
definition: BBa_K165031

BBa_K165003
type: Component
persistent identity: component1997408
display id: component1997408
version: 1
top level: BBa_K165101
access: public
definition: Sin3

BBa_K165011
type: Component
persistent identity: component1997405
display id: component1997405
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165004
type: Component
persistent identity: component1997410
display id: component1997410
version: 1
top level: BBa_K165101
access: public
definition: mCherry

BBa_K165006
type: Component
persistent identity: component1997409
display id: component1997409
version: 1
top level: BBa_K165101
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation1997409
display id: annotation1997409
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997409/range1997409/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K165101/annotation1997409/range1997409/1
type: Range
persistent identity: range1997409
display id: range1997409
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 3311
end: 3562
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997410
display id: annotation1997410
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997410/range1997410/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165101/annotation1997410/range1997410/1
type: Range
persistent identity: range1997410
display id: range1997410
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 3571
end: 4305
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997408
display id: annotation1997408
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997408/range1997408/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165101/annotation1997408/range1997408/1
type: Range
persistent identity: range1997408
display id: range1997408
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 492
end: 3302
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997412
display id: annotation1997412
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997412/range1997412/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165101/annotation1997412/range1997412/1
type: Range
persistent identity: range1997412
display id: range1997412
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 5057
end: 5309
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997407
display id: annotation1997407
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997407/range1997407/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165101/annotation1997407/range1997407/1
type: Range
persistent identity: range1997407
display id: range1997407
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 466
end: 483
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997406
display id: annotation1997406
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997406/range1997406/1
component: BBa_K165031

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type: Range
persistent identity: range1997406
display id: range1997406
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997405
display id: annotation1997405
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997405/range1997405/1
component: BBa_K165011

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type: Range
persistent identity: range1997405
display id: range1997405
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 1
end: 46
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997411
display id: annotation1997411
version: 1
top level: BBa_K165101
location: https://synbiohub.org/public/igem/BBa_K165101/annotation1997411/range1997411/1
component: BBa_K165004

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type: Range
persistent identity: range1997411
display id: range1997411
version: 1
direction: direction/reverse
top level: BBa_K165101
start: 4314
end: 5048
orientation: inline

BBa_K165090
type: ComponentDefinition
persistent identity: BBa K165090
display id: BBa_K165090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165003
component: BBa_K165009
component: BBa_K165002
component: BBa_K165004
component: BBa_K165012
component: BBa_K165031
component: BBa_K165018
sequence annotation: BBa_K165031
sequence annotation: BBa_K165004
sequence annotation: BBa_K165018
sequence annotation: BBa_K165012
sequence annotation: BBa_K165002
sequence annotation: BBa_K165009
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165090_sequence/1

BBa_K165003
type: Component
persistent identity: component1997226
display id: component1997226
version: 1
top level: BBa_K165090
access: public
definition: Sin3

BBa_K165009
type: Component
persistent identity: component1997225
display id: component1997225
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165002
type: Component
persistent identity: component1997224
display id: component1997224
version: 1
top level: BBa_K165090
access: public
definition: BBa_K165002

BBa_K165004
type: Component
persistent identity: component1997227
display id: component1997227
version: 1
top level: BBa_K165090
access: public
definition: mCherry

BBa_K165012
type: Component
persistent identity: component1997222
display id: component1997222
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165031
type: Component
persistent identity: component1997223
display id: component1997223
version: 1
top level: BBa_K165090
access: public
definition: BBa_K165031

BBa_K165018
type: Component
persistent identity: component1997229
display id: component1997229
version: 1
top level: BBa_K165090
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165004
type: Component
persistent identity: component1997228
display id: component1997228
version: 1
top level: BBa_K165090
access: public
definition: mCherry

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997223
display id: annotation1997223
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997223/range1997223/1
component: BBa_K165031

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type: Range
persistent identity: range1997223
display id: range1997223
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 136
end: 538
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997227
display id: annotation1997227
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997227/range1997227/1
component: BBa_K165004

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type: Range
persistent identity: range1997227
display id: range1997227
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 3658
end: 4392
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997229
display id: annotation1997229
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997229/range1997229/1
component: BBa_K165018

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type: Range
persistent identity: range1997229
display id: range1997229
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 5144
end: 5396
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997222
display id: annotation1997222
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997222/range1997222/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165090/annotation1997222/range1997222/1
type: Range
persistent identity: range1997222
display id: range1997222
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 1
end: 127
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997224
display id: annotation1997224
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997224/range1997224/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165090/annotation1997224/range1997224/1
type: Range
persistent identity: range1997224
display id: range1997224
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 547
end: 564
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997225
display id: annotation1997225
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997225/range1997225/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165090/annotation1997225/range1997225/1
type: Range
persistent identity: range1997225
display id: range1997225
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 573
end: 830
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997226
display id: annotation1997226
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997226/range1997226/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165090/annotation1997226/range1997226/1
type: Range
persistent identity: range1997226
display id: range1997226
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 839
end: 3649
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997228
display id: annotation1997228
version: 1
top level: BBa_K165090
location: https://synbiohub.org/public/igem/BBa_K165090/annotation1997228/range1997228/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165090/annotation1997228/range1997228/1
type: Range
persistent identity: range1997228
display id: range1997228
version: 1
direction: direction/reverse
top level: BBa_K165090
start: 4401
end: 5135
orientation: inline

BBa_K165021
type: ComponentDefinition
persistent identity: BBa K165021
display id: BBa_K165021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak + LexA Synthetic Activator (untagged)
creator: John Szymanski
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the kozak ribosome binding sequence ligated onto the LexA synthetic transcriptional activator (BBa_K165020).
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K165002
component: BBa_K165020
sequence annotation: BBa_K165002
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165021_sequence/1

BBa_K165002
type: Component
persistent identity: component1992128
display id: component1992128
version: 1
top level: BBa_K165021
access: public
definition: BBa_K165002

BBa_K165020
type: Component
persistent identity: component1992129
display id: component1992129
version: 1
top level: BBa_K165021
access: public
definition: lexA

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1992128
display id: annotation1992128
version: 1
top level: BBa_K165021
location: https://synbiohub.org/public/igem/BBa_K165021/annotation1992128/range1992128/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165021/annotation1992128/range1992128/1
type: Range
persistent identity: range1992128
display id: range1992128
version: 1
direction: direction/reverse
top level: BBa_K165021
start: 1
end: 18
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1992129
display id: annotation1992129
version: 1
top level: BBa_K165021
location: https://synbiohub.org/public/igem/BBa_K165021/annotation1992129/range1992129/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165021/annotation1992129/range1992129/1
type: Range
persistent identity: range1992129
display id: range1992129
version: 1
direction: direction/reverse
top level: BBa_K165021
start: 27
end: 698
orientation: inline

BBa_K1680017
type: ComponentDefinition
persistent identity: BBa K1680017
display id: BBa_K1680017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa Fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:17Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly.
mutable provenance: gBlocks designed by Team Tuebingen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1680006
sequence annotation: BBa_K1680007
sequence annotation: BBa_K1680000
sequence: https://synbiohub.org/public/igem/BBa_K1680017_sequence/1

BBa_K1680006
type: SequenceAnnotation
persistent identity: annotation2465979
display id: annotation2465979
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1

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type: Range
persistent identity: range2465979
display id: range2465979
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1064
end: 1732
orientation: inline

BBa_K1680007
type: SequenceAnnotation
persistent identity: annotation2465977
display id: annotation2465977
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1
type: Range
persistent identity: range2465977
display id: range2465977
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 13
end: 1041
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465978
display id: annotation2465978
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1
type: Range
persistent identity: range2465978
display id: range2465978
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1050
end: 1055
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465976
display id: annotation2465976
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1
type: Range
persistent identity: range2465976
display id: range2465976
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1
end: 6
orientation: inline

cfpx2
type: ComponentDefinition
persistent identity: BBa K165014
display id: BBa_K165014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP x2
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is 2 copies of the CFP gene fused to each other. The duplex FP tends to be more stable, and is used in synthetic transcription factors to provide a fluorescent output of concentration as well as stability.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165014_sequence/1

BBa_K1680020
type: ComponentDefinition
persistent identity: BBa K1680020
display id: BBa_K1680020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:16:19Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680003
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680020_sequence/1

K1680003
type: SequenceAnnotation
persistent identity: annotation2477365
display id: annotation2477365
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1
type: Range
persistent identity: range2477365
display id: range2477365
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1
end: 39
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477364
display id: annotation2477364
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1
type: Range
persistent identity: range2477364
display id: range2477364
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1114
end: 1782
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477366
display id: annotation2477366
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1
type: Range
persistent identity: range2477366
display id: range2477366
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1089
end: 1113
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477363
display id: annotation2477363
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1
type: Range
persistent identity: range2477363
display id: range2477363
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 40
end: 1068
orientation: inline

BBa_K106013
type: ComponentDefinition
persistent identity: BBa K106013
display id: BBa_K106013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Esa1 histone acetyltransferase, Aar1 BD part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the gene sequence for the Esa1 gene with AB ends for Aar1 cloning. Esa1 is a histone acetyltransferase (HAT).
mutable notes: Since this is an AB part, there is a ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106013_sequence/1

Venus
type: ComponentDefinition
persistent identity: BBa K1907000
display id: BBa_K1907000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus Yellow Fluorescent Protein
creator: Pihla Savola
created: 2016-10-11T11:00:00Z
modified: 2016-10-19T12:26:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29559
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1907000_sequence/1

BBa_K1907007
type: ComponentDefinition
persistent identity: BBa K1907007
display id: BBa_K1907007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TSA1 promoter + Venus YFP
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T02:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1907000
component: BBa_K1907004
sequence annotation: BBa_K1907004
sequence annotation: BBa_K1907000
sequence: https://synbiohub.org/public/igem/BBa_K1907007_sequence/1

BBa_K1907000
type: Component
persistent identity: component2522325
display id: component2522325
version: 1
top level: BBa_K1907007
access: public
definition: Venus

BBa_K1907004
type: Component
persistent identity: component2522324
display id: component2522324
version: 1
top level: BBa_K1907007
access: public
definition: BBa_K1907004

BBa_K1907004
type: SequenceAnnotation
persistent identity: annotation2522324
display id: annotation2522324
version: 1
top level: BBa_K1907007
location: https://synbiohub.org/public/igem/BBa_K1907007/annotation2522324/range2522324/1
component: BBa_K1907004

https://synbiohub.org/public/igem/BBa_K1907007/annotation2522324/range2522324/1
type: Range
persistent identity: range2522324
display id: range2522324
version: 1
direction: direction/reverse
top level: BBa_K1907007
start: 1
end: 260
orientation: inline

BBa_K1907000
type: SequenceAnnotation
persistent identity: annotation2522325
display id: annotation2522325
version: 1
top level: BBa_K1907007
location: https://synbiohub.org/public/igem/BBa_K1907007/annotation2522325/range2522325/1
component: BBa_K1907000

https://synbiohub.org/public/igem/BBa_K1907007/annotation2522325/range2522325/1
type: Range
persistent identity: range2522325
display id: range2522325
version: 1
direction: direction/reverse
top level: BBa_K1907007
start: 267
end: 983
orientation: inline

BBa_K165075
type: ComponentDefinition
persistent identity: BBa K165075
display id: BBa_K165075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA repressible reporter on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165075_sequence/1

BBa_I766555
type: ComponentDefinition
persistent identity: BBa I766555
display id: BBa_I766555
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCyc (Medium) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mid-expression level constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Cytochrome c
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766555_sequence/1

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MET 25 Promoter
creator: John Szymanski
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
mutable notes: None.
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

BBa_K1680019
type: ComponentDefinition
persistent identity: BBa K1680019
display id: BBa_K1680019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:55Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680006
sequence annotation: K1680007
sequence annotation: K1680002
sequence: https://synbiohub.org/public/igem/BBa_K1680019_sequence/1

K1680006
type: SequenceAnnotation
persistent identity: annotation2477418
display id: annotation2477418
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1
type: Range
persistent identity: range2477418
display id: range2477418
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1096
end: 1764
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477416
display id: annotation2477416
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1
type: Range
persistent identity: range2477416
display id: range2477416
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 31
end: 1059
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477417
display id: annotation2477417
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1
type: Range
persistent identity: range2477417
display id: range2477417
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1060
end: 1095
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477415
display id: annotation2477415
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1
type: Range
persistent identity: range2477415
display id: range2477415
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1
end: 30
orientation: inline

BBa_K801090
type: ComponentDefinition
persistent identity: BBa K801090
display id: BBa_K801090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phenylalanine ammonia lyase (PAL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: phenylalanine ammonia lyase (PAL) coding region from ''Rhodosporidium toruloides'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nuccore/AX366866 PMID: 1593299
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Phe instead of Gly
sequence annotation: Gly instead of Glu
sequence annotation: Arg instead of Lys
sequence annotation: Consensus sequence
sequence annotation: PAL
sequence: https://synbiohub.org/public/igem/BBa_K801090_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2201779
display id: annotation2201779
version: 1
top level: BBa_K801090
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201779/range2201779/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201779/range2201779/1
type: Range
persistent identity: range2201779
display id: range2201779
version: 1
direction: direction/forward
top level: BBa_K801090
start: 7
end: 9
orientation: inline

Phe instead of Gly
type: SequenceAnnotation
persistent identity: annotation2201780
display id: annotation2201780
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201780/range2201780/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201780/range2201780/1
type: Range
persistent identity: range2201780
display id: range2201780
version: 1
direction: direction/forward
top level: BBa_K801090
start: 338
end: 339
orientation: inline

Gly instead of Glu
type: SequenceAnnotation
persistent identity: annotation2201793
display id: annotation2201793
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201793/range2201793/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201793/range2201793/1
type: Range
persistent identity: range2201793
display id: range2201793
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1214
end: 1214
orientation: inline

Arg instead of Lys
type: SequenceAnnotation
persistent identity: annotation2201784
display id: annotation2201784
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201784/range2201784/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201784/range2201784/1
type: Range
persistent identity: range2201784
display id: range2201784
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1007
end: 1007
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2201778
display id: annotation2201778
version: 1
top level: BBa_K801090
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201778/range2201778/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201778/range2201778/1
type: Range
persistent identity: range2201778
display id: range2201778
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1
end: 6
orientation: inline

PAL
type: SequenceAnnotation
persistent identity: annotation2201808
display id: annotation2201808
version: 1
top level: BBa_K801090
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201808/range2201808/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201808/range2201808/1
type: Range
persistent identity: range2201808
display id: range2201808
version: 1
direction: direction/forward
top level: BBa_K801090
start: 7
end: 2154
orientation: inline

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: Strep Tag
sequence annotation: Consensus sequence
sequence annotation: CaDXMT1
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 9
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1189
end: 1194
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1159
end: 1188
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1
end: 6
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 1158
orientation: inline

BBa_K1486030
type: ComponentDefinition
persistent identity: BBa K1486030
display id: BBa_K1486030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucN + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486024
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486030_sequence/1

BBa_K1486019
type: Component
persistent identity: component2417717
display id: component2417717
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486025
type: Component
persistent identity: component2417718
display id: component2417718
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2417716
display id: component2417716
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417722
display id: component2417722
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486024

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417716
display id: annotation2417716
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
type: Range
persistent identity: range2417716
display id: range2417716
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417718
display id: annotation2417718
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
type: Range
persistent identity: range2417718
display id: range2417718
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 370
end: 557
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417722
display id: annotation2417722
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
type: Range
persistent identity: range2417722
display id: range2417722
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 558
end: 1914
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417717
display id: annotation2417717
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
type: Range
persistent identity: range2417717
display id: range2417717
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 40
end: 369
orientation: inline

BBa_K1861030
type: ComponentDefinition
persistent identity: BBa K1861030
display id: BBa_K1861030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP gene for expression in yeast
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:49:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J63002
component: BBa_K1861301
component: BBa_K1861303
component: BBa_K1861304
component: BBa_K792001
component: BBa_K1861302
sequence annotation: BBa_K1861301
sequence annotation: BBa_J63002
sequence annotation: BBa_K1861304
sequence annotation: BBa_K1861303
sequence annotation: BBa_K792001
sequence annotation: BBa_K1861302
sequence: https://synbiohub.org/public/igem/BBa_K1861030_sequence/1

BBa_J63002
type: Component
persistent identity: component2474915
display id: component2474915
version: 1
top level: BBa_K1861030
access: public
definition: tADH1

BBa_K1861301
type: Component
persistent identity: component2474907
display id: component2474907
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861301/1

BBa_K1861303
type: Component
persistent identity: component2474908
display id: component2474908
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861303/1

BBa_K1861304
type: Component
persistent identity: component2474916
display id: component2474916
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861304/1

BBa_K792001
type: Component
persistent identity: component2474912
display id: component2474912
version: 1
top level: BBa_K1861030
access: public
definition: MFa1-Kozak

BBa_K1861302
type: Component
persistent identity: component2474913
display id: component2474913
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861302/1

BBa_K1861301
type: SequenceAnnotation
persistent identity: annotation2474907
display id: annotation2474907
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474907/range2474907/1
component: BBa_K1861301

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474907/range2474907/1
type: Range
persistent identity: range2474907
display id: range2474907
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1
end: 71
orientation: inline

BBa_J63002
type: SequenceAnnotation
persistent identity: annotation2474915
display id: annotation2474915
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
component: BBa_J63002

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
type: Range
persistent identity: range2474915
display id: range2474915
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1062
end: 1286
orientation: inline

BBa_K1861304
type: SequenceAnnotation
persistent identity: annotation2474916
display id: annotation2474916
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474916/range2474916/1
component: BBa_K1861304

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474916/range2474916/1
type: Range
persistent identity: range2474916
display id: range2474916
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1287
end: 1349
orientation: inline

BBa_K1861303
type: SequenceAnnotation
persistent identity: annotation2474908
display id: annotation2474908
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474908/range2474908/1
component: BBa_K1861303

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474908/range2474908/1
type: Range
persistent identity: range2474908
display id: range2474908
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 72
end: 275
orientation: inline

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2474912
display id: annotation2474912
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474912/range2474912/1
component: BBa_K792001

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474912/range2474912/1
type: Range
persistent identity: range2474912
display id: range2474912
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 276
end: 296
orientation: inline

BBa_K1861302
type: SequenceAnnotation
persistent identity: annotation2474913
display id: annotation2474913
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
component: BBa_K1861302

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
type: Range
persistent identity: range2474913
display id: range2474913
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 297
end: 1061
orientation: inline

BBa_K165077
type: ComponentDefinition
persistent identity: BBa K165077
display id: BBa_K165077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 repressible reporter
creator: Aaron Glieberman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: Silver lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165077_sequence/1

BBa_K106672
type: ComponentDefinition
persistent identity: BBa K106672
display id: BBa_K106672
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS305, Gal1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Gal1 promoter (Gal1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS305 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS305 plasmid--Integrating yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Gal1 promoter
sequence annotation: Adh1 terminator
sequence annotation: Start codon
sequence annotation: LEU2 marker
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: lac promoter
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: T7 promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: T3 promoter
sequence annotation: A overhang
sequence annotation: ampicillin resistance promoter
sequence annotation: D overhang
sequence annotation: Gal1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K106672_sequence/1

Gal1 promoter
type: SequenceAnnotation
persistent identity: annotation1999712
display id: annotation1999712
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1
type: Range
persistent identity: range1999712
display id: range1999712
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6172
end: 6621
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1993688
display id: annotation1993688
version: 1
top level: BBa_K106672
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1
type: Range
persistent identity: range1993688
display id: range1993688
version: 1
direction: direction/forward
top level: BBa_K106672
start: 30
end: 215
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999714
display id: annotation1999714
version: 1
top level: BBa_K106672
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1
type: Range
persistent identity: range1999714
display id: range1999714
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6626
end: 6628
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1993684
display id: annotation1993684
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1
type: Range
persistent identity: range1993684
display id: range1993684
version: 1
direction: direction/forward
top level: BBa_K106672
start: 1570
end: 2676
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999707
display id: annotation1999707
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1
type: Range
persistent identity: range1999707
display id: range1999707
version: 1
direction: direction/forward
top level: BBa_K106672
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999706
display id: annotation1999706
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1
type: Range
persistent identity: range1999706
display id: range1999706
version: 1
direction: direction/forward
top level: BBa_K106672
start: 246
end: 405
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999710
display id: annotation1999710
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1
type: Range
persistent identity: range1999710
display id: range1999710
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5482
end: 5511
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1993685
display id: annotation1993685
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1
type: Range
persistent identity: range1993685
display id: range1993685
version: 1
direction: direction/forward
top level: BBa_K106672
start: 3539
end: 4399
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999709
display id: annotation1999709
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1
type: Range
persistent identity: range1999709
display id: range1999709
version: 1
direction: direction/forward
top level: BBa_K106672
start: 4554
end: 5173
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999705
display id: annotation1999705
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999705/range1999705/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999705/range1999705/1
type: Range
persistent identity: range1999705
display id: range1999705
version: 1
direction: direction/forward
top level: BBa_K106672
start: 224
end: 242
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999713
display id: annotation1999713
version: 1
top level: BBa_K106672
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999713/range1999713/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999713/range1999713/1
type: Range
persistent identity: range1999713
display id: range1999713
version: 1
direction: direction/forward
top level: BBa_K106672
start: 11
end: 21
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999711
display id: annotation1999711
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999711/range1999711/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999711/range1999711/1
type: Range
persistent identity: range1999711
display id: range1999711
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5581
end: 5600
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999715
display id: annotation1999715
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999715/range1999715/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999715/range1999715/1
type: Range
persistent identity: range1999715
display id: range1999715
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6635
end: 6638
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999708
display id: annotation1999708
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999708/range1999708/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999708/range1999708/1
type: Range
persistent identity: range1999708
display id: range1999708
version: 1
direction: direction/forward
top level: BBa_K106672
start: 3469
end: 3497
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1993689
display id: annotation1993689
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993689/range1993689/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993689/range1993689/1
type: Range
persistent identity: range1993689
display id: range1993689
version: 1
direction: direction/forward
top level: BBa_K106672
start: 1
end: 4
orientation: inline

Gal1 Promoter
type: SequenceAnnotation
persistent identity: annotation1993687
display id: annotation1993687
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993687/range1993687/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993687/range1993687/1
type: Range
persistent identity: range1993687
display id: range1993687
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5618
end: 6634
orientation: inline

BBa_K801096
type: ComponentDefinition
persistent identity: BBa K801096
display id: BBa_K801096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aromatic prenyltransferase (APT) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Aromatic prenyltransferase (APT) coding region from ''Humulus lupulus'', yeast codon optimized.
mutable notes: -
mutable provenance: Synthesized by Geneart, yeast codon optimized http://www.ncbi.nlm.nih.gov/nuccore/AB543053
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: APT
sequence: https://synbiohub.org/public/igem/BBa_K801096_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202205
display id: annotation2202205
version: 1
top level: BBa_K801096
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801096/annotation2202205/range2202205/1

https://synbiohub.org/public/igem/BBa_K801096/annotation2202205/range2202205/1
type: Range
persistent identity: range2202205
display id: range2202205
version: 1
direction: direction/forward
top level: BBa_K801096
start: 1
end: 3
orientation: inline

APT
type: SequenceAnnotation
persistent identity: annotation2202207
display id: annotation2202207
version: 1
top level: BBa_K801096
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801096/annotation2202207/range2202207/1

https://synbiohub.org/public/igem/BBa_K801096/annotation2202207/range2202207/1
type: Range
persistent identity: range2202207
display id: range2202207
version: 1
direction: direction/forward
top level: BBa_K801096
start: 1
end: 1233
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E2050
display id: BBa_E2050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2016-01-25T02:35:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 4206
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP derivative. Ex548nm/Em562. yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: mOrange
sequence annotation: GSGTA linker
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2050_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214024
display id: annotation2214024
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1

https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
type: Range
persistent identity: range2214024
display id: range2214024
version: 1
direction: direction/forward
top level: mRFP1
start: 745
end: 769
orientation: inline

mOrange
type: SequenceAnnotation
persistent identity: annotation1431920
display id: annotation1431920
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
type: Range
persistent identity: range1431920
display id: range1431920
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 738
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431919
display id: annotation1431919
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
type: Range
persistent identity: range1431919
display id: range1431919
version: 1
direction: direction/forward
top level: mRFP1
start: 724
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431915
display id: annotation1431915
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
type: Range
persistent identity: range1431915
display id: range1431915
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 15
orientation: inline

BBa_K1486026
type: ComponentDefinition
persistent identity: BBa K1486026
display id: BBa_K1486026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + Kanamycin resistance for yeast
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Adh1 terminator
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486023
sequence annotation: Pbs2 flanking
sequence annotation: 8 a.a linker
sequence: https://synbiohub.org/public/igem/BBa_K1486026_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2417050
display id: annotation2417050
version: 1
top level: BBa_K1486026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1
type: Range
persistent identity: range2417050
display id: range2417050
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 811
end: 999
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417051
display id: annotation2417051
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1
type: Range
persistent identity: range2417051
display id: range2417051
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1073
end: 2430
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417049
display id: annotation2417049
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1
type: Range
persistent identity: range2417049
display id: range2417049
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 75
end: 791
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417052
display id: annotation2417052
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1
type: Range
persistent identity: range2417052
display id: range2417052
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 2485
end: 2535
orientation: inline

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417048
display id: annotation2417048
version: 1
top level: BBa_K1486026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1
type: Range
persistent identity: range2417048
display id: range2417048
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 51
end: 74
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417047
display id: annotation2417047
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1
type: Range
persistent identity: range2417047
display id: range2417047
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1
end: 50
orientation: inline

BBa_K1486024
type: ComponentDefinition
persistent identity: BBa K1486024
display id: BBa_K1486024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF terminator
sequence annotation: KanR
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486024_sequence/1

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2416944
display id: annotation2416944
version: 1
top level: BBa_K1486024
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1
type: Range
persistent identity: range2416944
display id: range2416944
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1159
end: 1357
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2416943
display id: annotation2416943
version: 1
top level: BBa_K1486024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1
type: Range
persistent identity: range2416943
display id: range2416943
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 345
end: 1154
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2416942
display id: annotation2416942
version: 1
top level: BBa_K1486024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1
type: Range
persistent identity: range2416942
display id: range2416942
version: 1
direction: direction/forward
top level: BBa_K1486024
start: 1
end: 344
orientation: inline

BBa_K753000
type: ComponentDefinition
persistent identity: BBa K753000
display id: BBa_K753000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast FIG1 promoter
creator: Rachel Engeler
created: 2012-08-28T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _1004_
m user id: 0
owner id: 11572
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter of the FIG1 gene in yeast. The transcription factor Ste12, activated in the yeast pheromone pathway, binds to the FIG1 promoter and initiate the expression of the FIG1 gene. Five pheromone response elements (PRE, consensus sequence ATGAAACA) have been identifi?ed in the 5'-upstream regulatory sequence of the FIG1 gene. Phosphorylated Ste12 binds to them and thereby activates transcription. The PREs in the FIG1 promoter di?ffer slightly from the PRE consensus sequence ATGAAACA: Sequence Location TGAAACG -405 TGAAACC* -217 TGACACA* -206 TGAAACG -173 TGAAATA -133 Sites marked with an asterisk occur on the opposite strand The strictly regulated transcriptional feature of the FIG1 promoter can be useful when investigating the pheromone pathway. The promoter can be used to express reporter genes in response to activation of the pheromone pathway. Reference: Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated Genes Required for Yeast Mating Diff?erentiation. The Journal of cell biology. 1998;140(3):461-483.
mutable notes: The length of the promoter was chosen to 450 bp as recommended by Kondo group of Kobe University of Japan. Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem. 2010 Jun;147(6):867-73
mutable provenance: The sequence has been amplified directly from genomic yeast DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K753000_sequence/1

BBa_K392001
type: ComponentDefinition
persistent identity: BBa K392001
display id: BBa_K392001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ENO2 promoter
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-09-25T08:35:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _528_
m user id: 15693
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LacI promoter with RBS attached downstream.
mutable notes: Assembled by 3A method.
mutable provenance: Registry parts BBa_R0010 (promoter) and BBa_B0034 (RBS).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K392001_sequence/1

BBa_K950008
type: ComponentDefinition
persistent identity: BBa K950008
display id: BBa_K950008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 shuttle vector
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pRS313 yeast plasmid
mutable notes: contains restriction sites needed for assembly of BioBricks
mutable provenance: Jansen lab Tuebingen University
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: lacZ
sequence annotation: F1_ORI
sequence annotation: T3_P
sequence annotation: ARSH4
sequence annotation: MCS
sequence annotation: PMB1 ORI
sequence annotation: CEN6
sequence annotation: SpeI
sequence annotation: bla
sequence annotation: HIS3
sequence annotation: XbaI
sequence annotation: T7_P
sequence: https://synbiohub.org/public/igem/BBa_K950008_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179278
display id: annotation2179278
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179278/range2179278/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179278/range2179278/1
type: Range
persistent identity: range2179278
display id: range2179278
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 1880
end: 2217
orientation: inline

F1_ORI
type: SequenceAnnotation
persistent identity: annotation2179287
display id: annotation2179287
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179287/range2179287/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179287/range2179287/1
type: Range
persistent identity: range2179287
display id: range2179287
version: 1
direction: direction/forward
top level: BBa_K950008
start: 1647
end: 1746
orientation: inline

T3_P
type: SequenceAnnotation
persistent identity: annotation2179286
display id: annotation2179286
version: 1
top level: BBa_K950008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179286/range2179286/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179286/range2179286/1
type: Range
persistent identity: range2179286
display id: range2179286
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 2192
end: 2211
orientation: inline

ARSH4
type: SequenceAnnotation
persistent identity: annotation2179284
display id: annotation2179284
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179284/range2179284/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179284/range2179284/1
type: Range
persistent identity: range2179284
display id: range2179284
version: 1
direction: direction/forward
top level: BBa_K950008
start: 4512
end: 4885
orientation: inline

MCS
type: SequenceAnnotation
persistent identity: annotation2179282
display id: annotation2179282
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179282/range2179282/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179282/range2179282/1
type: Range
persistent identity: range2179282
display id: range2179282
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2073
end: 2174
orientation: inline

PMB1 ORI
type: SequenceAnnotation
persistent identity: annotation2179288
display id: annotation2179288
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179288/range2179288/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179288/range2179288/1
type: Range
persistent identity: range2179288
display id: range2179288
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2635
end: 2635
orientation: inline

CEN6
type: SequenceAnnotation
persistent identity: annotation2179283
display id: annotation2179283
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179283/range2179283/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179283/range2179283/1
type: Range
persistent identity: range2179283
display id: range2179283
version: 1
direction: direction/forward
top level: BBa_K950008
start: 4395
end: 4511
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2179280
display id: annotation2179280
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179280/range2179280/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179280/range2179280/1
type: Range
persistent identity: range2179280
display id: range2179280
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2103
end: 2108
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179279
display id: annotation2179279
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179279/range2179279/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179279/range2179279/1
type: Range
persistent identity: range2179279
display id: range2179279
version: 1
direction: direction/reverse
top level: BBa_K950008
start: 3393
end: 4253
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2179277
display id: annotation2179277
version: 1
top level: BBa_K950008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179277/range2179277/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179277/range2179277/1
type: Range
persistent identity: range2179277
display id: range2179277
version: 1
direction: direction/forward
top level: BBa_K950008
start: 503
end: 1162
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2179281
display id: annotation2179281
version: 1
top level: BBa_K950008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179281/range2179281/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179281/range2179281/1
type: Range
persistent identity: range2179281
display id: range2179281
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2097
end: 2102
orientation: inline

T7_P
type: SequenceAnnotation
persistent identity: annotation2179285
display id: annotation2179285
version: 1
top level: BBa_K950008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K950008/annotation2179285/range2179285/1

https://synbiohub.org/public/igem/BBa_K950008/annotation2179285/range2179285/1
type: Range
persistent identity: range2179285
display id: range2179285
version: 1
direction: direction/forward
top level: BBa_K950008
start: 2046
end: 2065
orientation: inline

BBa_K1680023
type: ComponentDefinition
persistent identity: BBa K1680023
display id: BBa_K1680023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:42:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680004
sequence annotation: K1680002
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680023_sequence/1

K1680004
type: SequenceAnnotation
persistent identity: annotation2477379
display id: annotation2477379
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1
type: Range
persistent identity: range2477379
display id: range2477379
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1
end: 24
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477384
display id: annotation2477384
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1
type: Range
persistent identity: range2477384
display id: range2477384
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1765
end: 1800
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477383
display id: annotation2477383
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1
type: Range
persistent identity: range2477383
display id: range2477383
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 700
end: 735
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477380
display id: annotation2477380
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1
type: Range
persistent identity: range2477380
display id: range2477380
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 31
end: 699
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477381
display id: annotation2477381
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1
type: Range
persistent identity: range2477381
display id: range2477381
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1801
end: 2469
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477382
display id: annotation2477382
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1
type: Range
persistent identity: range2477382
display id: range2477382
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 736
end: 1764
orientation: inline

BBa_K165032
type: ComponentDefinition
persistent identity: BBa K165032
display id: BBa_K165032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Gli1 binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165032_sequence/1

REX
type: ComponentDefinition
persistent identity: BBa K809401
display id: BBa_K809401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: REX coding sequence
creator: Xiaopeng Xu
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1066_
m user id: 0
owner id: 12018
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick is the coding sequence of our SU9+REX in yeast cytoplasm. It is used to construct our final biobricks which express REX in the cytoplasm and then transport REX into mitochondrial matrix.
mutable notes: The signal peptid must be able to work efficiently, and can transport REX into mitochondrial matrix.
mutable provenance: SU9 comes from yeast gemome. REX was modified to suitable for expression in yeast.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REX
sequence: https://synbiohub.org/public/igem/BBa_K809401_sequence/1

REX
type: SequenceAnnotation
persistent identity: annotation2188980
display id: annotation2188980
version: 1
top level: REX
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K809401/annotation2188980/range2188980/1

https://synbiohub.org/public/igem/BBa_K809401/annotation2188980/range2188980/1
type: Range
persistent identity: range2188980
display id: range2188980
version: 1
direction: direction/forward
top level: REX
start: 1
end: 774
orientation: inline

BBa_K1462010
type: ComponentDefinition
persistent identity: BBa K1462010
display id: BBa_K1462010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: coxIV
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CoxVI is the N-terminal mitochondrial localization signal from subunit IV of the yeast cytochrome c oxidase.
mutable notes: 1
mutable provenance: Saccharomyces cerevisiae genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462010_sequence/1

BBa_K1907003
type: ComponentDefinition
persistent identity: BBa K1907003
display id: BBa_K1907003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CTT1 promoter for S. cerevisiae
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T03:16:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1907003_sequence/1

BBa_K976009
type: ComponentDefinition
persistent identity: BBa K976009
display id: BBa_K976009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TDH3 Promoter + Yeast Kozak + FGFR-1/FRS2 Complex +TEF1 Terminator
creator: Amna Sohail
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description:  BioBrick Source & Functions: A. Promoter: BBa_124002; Yeast GPD (TDH3) Promoter Description: The Yeast GPD (TDH3) promoter was designed by John Szymanski of the 2008 Brown Group. This constitutive promoter is still in the planning phase and is 681 base pairs in length. B. Ribosome Binding Site: BBa_J63003; Yeast Kozak Sequence Description: The ribosome binding site was designed by Caroline Ajo-Franklin of the 2006 Silver Lab Group. The BioBrick presents a yeast kozak sequence (ribosome binding site) that is 18 base pairs in length. C. Coding Sequence: BBa_K976000; Fibroblast Growth Factor Receptor-1 Description: The Fibroblast Growth Factor Receptor-1 (FGFR-1) BioBrick has been designed by Amna Sohail and Meredith Gentry of the 2013 MCIT Indianapolis Group. This BioBrick presents a receptor that has a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor. The BioBrick itself is in the planning phase and is 2,466 base pairs in length. D. Coding Sequence: BBa_K976001; Fibroblast Growth Factor Receptor Substrate 2 Description: The Fibroblast Growth Factor Receptor Substrate 2 (FRS2) BioBrick has been designed by Amna Sohail of the 2013 MCIT Indianapolis Group. The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that is involved in melanocyte proliferation, differentiation, and migration. FRS2 may be utilized to mediate downstream signaling to activate luciferase production after the FGF2 ligand binds to FGFR-1. This BioBrick is 1,524 base pairs in length. E. Terminator: BBa_K801011; TEF1 yeast terminator Description: The TEF1 Yeast Terminator…
mutable notes: None.
mutable provenance: NCBI GenBank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K124002
component: BBa_K976002
component: BBa_J63003
component: BBa_K801011
sequence annotation: BBa_J63003
sequence annotation: BBa_K976002
sequence annotation: BBa_K124002
sequence annotation: BBa_K801011
sequence: https://synbiohub.org/public/igem/BBa_K976009_sequence/1

BBa_K124002
type: Component
persistent identity: component2329837
display id: component2329837
version: 1
top level: BBa_K976009
access: public
definition: BBa_K124002

BBa_K976002
type: Component
persistent identity: component2329842
display id: component2329842
version: 1
top level: BBa_K976009
access: public
definition: https://synbiohub.org/public/igem/BBa_K976002/1

BBa_J63003
type: Component
persistent identity: component2329839
display id: component2329839
version: 1
top level: BBa_K976009
access: public
definition: Kozak & st

BBa_K801011
type: Component
persistent identity: component2329844
display id: component2329844
version: 1
top level: BBa_K976009
access: public
definition: BBa_K801011

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2329839
display id: annotation2329839
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329839/range2329839/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_K976009/annotation2329839/range2329839/1
type: Range
persistent identity: range2329839
display id: range2329839
version: 1
direction: direction/reverse
top level: BBa_K976009
start: 690
end: 707
orientation: inline

BBa_K976002
type: SequenceAnnotation
persistent identity: annotation2329842
display id: annotation2329842
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329842/range2329842/1
component: BBa_K976002

https://synbiohub.org/public/igem/BBa_K976009/annotation2329842/range2329842/1
type: Range
persistent identity: range2329842
display id: range2329842
version: 1
direction: direction/reverse
top level: BBa_K976009
start: 714
end: 4709
orientation: inline

BBa_K124002
type: SequenceAnnotation
persistent identity: annotation2329837
display id: annotation2329837
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329837/range2329837/1
component: BBa_K124002

https://synbiohub.org/public/igem/BBa_K976009/annotation2329837/range2329837/1
type: Range
persistent identity: range2329837
display id: range2329837
version: 1
direction: direction/reverse
top level: BBa_K976009
start: 1
end: 681
orientation: inline

BBa_K801011
type: SequenceAnnotation
persistent identity: annotation2329844
display id: annotation2329844
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329844/range2329844/1
component: BBa_K801011

https://synbiohub.org/public/igem/BBa_K976009/annotation2329844/range2329844/1
type: Range
persistent identity: range2329844
display id: range2329844
version: 1
direction: direction/reverse
top level: BBa_K976009
start: 4718
end: 5224
orientation: inline

BBa_K950005
type: ComponentDefinition
persistent identity: BBa K950005
display id: BBa_K950005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lacZ can be used as reporter gene
mutable notes: no Xba1 Spe1 sites found
mutable provenance: plasmid from Jansen lab Tuebingen University
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ
sequence: https://synbiohub.org/public/igem/BBa_K950005_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179274
display id: annotation2179274
version: 1
top level: BBa_K950005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950005/annotation2179274/range2179274/1

https://synbiohub.org/public/igem/BBa_K950005/annotation2179274/range2179274/1
type: Range
persistent identity: range2179274
display id: range2179274
version: 1
direction: direction/forward
top level: BBa_K950005
start: 1
end: 2514
orientation: inline

BBa_K105002
type: ComponentDefinition
persistent identity: BBa K105002
display id: BBa_K105002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TUP1 - repressor domain for transcription factors in yeast
creator: Sandra Dittmann
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is the N-terminal repression domain of the yeast general repressor TUP1.<br\n> In artificial systems TUP1 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective repressionn of genes with a promoter containing this bait sequence can be achieved.
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick.<br\n> For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." - DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Yeast genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105002_sequence/1

BBa_K165081
type: ComponentDefinition
persistent identity: BBa K165081
display id: BBa_K165081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Untagged Zif268-HIV activator on pRS305
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165022
component: BBa_K165046
sequence annotation: BBa_K165022
sequence annotation: BBa_K165046
sequence: https://synbiohub.org/public/igem/BBa_K165081_sequence/1

BBa_K165022
type: Component
persistent identity: component1997138
display id: component1997138
version: 1
top level: BBa_K165081
access: public
definition: BBa_K165022

BBa_K165046
type: Component
persistent identity: component1997137
display id: component1997137
version: 1
top level: BBa_K165081
access: public
definition: BBa_K165046

BBa_K165022
type: SequenceAnnotation
persistent identity: annotation1997138
display id: annotation1997138
version: 1
top level: BBa_K165081
location: https://synbiohub.org/public/igem/BBa_K165081/annotation1997138/range1997138/1
component: BBa_K165022

https://synbiohub.org/public/igem/BBa_K165081/annotation1997138/range1997138/1
type: Range
persistent identity: range1997138
display id: range1997138
version: 1
direction: direction/reverse
top level: BBa_K165081
start: 558
end: 1223
orientation: inline

BBa_K165046
type: SequenceAnnotation
persistent identity: annotation1997137
display id: annotation1997137
version: 1
top level: BBa_K165081
location: https://synbiohub.org/public/igem/BBa_K165081/annotation1997137/range1997137/1
component: BBa_K165046

https://synbiohub.org/public/igem/BBa_K165081/annotation1997137/range1997137/1
type: Range
persistent identity: range1997137
display id: range1997137
version: 1
direction: direction/reverse
top level: BBa_K165081
start: 1
end: 549
orientation: inline

BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xanthosine methyltransferase CaXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CaXMT1
sequence annotation: Consensus sequence
sequence annotation: Start codon
sequence annotation: Stop codon
sequence annotation: Strep Tag
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 1122
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1
end: 6
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
type: Range
persistent identity: range2198399
display id: range2198399
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 9
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
persistent identity: range2198401
display id: range2198401
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1153
end: 1158
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1123
end: 1152
orientation: inline

BBa_K284003
type: ComponentDefinition
persistent identity: BBa K284003
display id: BBa_K284003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Partial DLD Promoter from Kluyveromyces lactis
creator: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: TATA-Like
sequence annotation: DLD Promoter
sequence: https://synbiohub.org/public/igem/BBa_K284003_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034973
display id: annotation2034973
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1
type: Range
persistent identity: range2034973
display id: range2034973
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 22
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034976
display id: annotation2034976
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1
type: Range
persistent identity: range2034976
display id: range2034976
version: 1
direction: direction/reverse
top level: BBa_K284003
start: 442
end: 463
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034974
display id: annotation2034974
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1
type: Range
persistent identity: range2034974
display id: range2034974
version: 1
direction: direction/forward
top level: BBa_K284003
start: 300
end: 330
orientation: inline

DLD Promoter
type: SequenceAnnotation
persistent identity: annotation2034972
display id: annotation2034972
version: 1
top level: BBa_K284003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1
type: Range
persistent identity: range2034972
display id: range2034972
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 463
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034975
display id: annotation2034975
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1
type: Range
persistent identity: range2034975
display id: range2034975
version: 1
direction: direction/forward
top level: BBa_K284003
start: 355
end: 372
orientation: inline

BBa_K165098
type: ComponentDefinition
persistent identity: BBa K165098
display id: BBa_K165098
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + ADH1 + Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165018
component: BBa_K165015
component: BBa_K165007
component: BBa_K165011
component: BBa_K165003
component: BBa_K165002
component: BBa_K165014
sequence annotation: BBa_K165018
sequence annotation: BBa_K165014
sequence annotation: BBa_K165003
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165098_sequence/1

BBa_K165018
type: Component
persistent identity: component1997389
display id: component1997389
version: 1
top level: BBa_K165098
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165015
type: Component
persistent identity: component1997384
display id: component1997384
version: 1
top level: BBa_K165098
access: public
definition: BBa_K165015

BBa_K165007
type: Component
persistent identity: component1997387
display id: component1997387
version: 1
top level: BBa_K165098
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165011
type: Component
persistent identity: component1997383
display id: component1997383
version: 1
top level: BBa_K165098
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165003
type: Component
persistent identity: component1997386
display id: component1997386
version: 1
top level: BBa_K165098
access: public
definition: Sin3

BBa_K165002
type: Component
persistent identity: component1997385
display id: component1997385
version: 1
top level: BBa_K165098
access: public
definition: BBa_K165002

BBa_K165014
type: Component
persistent identity: component1997388
display id: component1997388
version: 1
top level: BBa_K165098
access: public
definition: cfpx2

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997389
display id: annotation1997389
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997389/range1997389/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165098/annotation1997389/range1997389/1
type: Range
persistent identity: range1997389
display id: range1997389
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 6388
end: 6640
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997388
display id: annotation1997388
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997388/range1997388/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165098/annotation1997388/range1997388/1
type: Range
persistent identity: range1997388
display id: range1997388
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 4886
end: 6379
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997386
display id: annotation1997386
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997386/range1997386/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165098/annotation1997386/range1997386/1
type: Range
persistent identity: range1997386
display id: range1997386
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 1534
end: 4344
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997387
display id: annotation1997387
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997387/range1997387/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165098/annotation1997387/range1997387/1
type: Range
persistent identity: range1997387
display id: range1997387
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 4353
end: 4877
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997383
display id: annotation1997383
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997383/range1997383/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165098/annotation1997383/range1997383/1
type: Range
persistent identity: range1997383
display id: range1997383
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 1
end: 46
orientation: inline

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1997384
display id: annotation1997384
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997384/range1997384/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165098/annotation1997384/range1997384/1
type: Range
persistent identity: range1997384
display id: range1997384
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 55
end: 1499
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997385
display id: annotation1997385
version: 1
top level: BBa_K165098
location: https://synbiohub.org/public/igem/BBa_K165098/annotation1997385/range1997385/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165098/annotation1997385/range1997385/1
type: Range
persistent identity: range1997385
display id: range1997385
version: 1
direction: direction/reverse
top level: BBa_K165098
start: 1508
end: 1525
orientation: inline

BBa_I766558
type: ComponentDefinition
persistent identity: BBa I766558
display id: BBa_I766558
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig1 (Inducible) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Inducible promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Fig1 (Factor Induced Gene 1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766558_sequence/1

BBa_K394003
type: ComponentDefinition
persistent identity: BBa K394003
display id: BBa_K394003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sex-Lethal from Drosophila melanogaster
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the coding region for the Sex-Lethal Gene from which can be used to control splicing events.
mutable notes: none
mutable provenance: This was part was made using PCR from a construct synthesized by Mr. Gene. It intons have been artificially removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Sxl gene
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K394003_sequence/1

Sxl gene
type: SequenceAnnotation
persistent identity: annotation2102751
display id: annotation2102751
version: 1
top level: BBa_K394003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394003/annotation2102751/range2102751/1

https://synbiohub.org/public/igem/BBa_K394003/annotation2102751/range2102751/1
type: Range
persistent identity: range2102751
display id: range2102751
version: 1
direction: direction/forward
top level: BBa_K394003
start: 1
end: 1062
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2095519
display id: annotation2095519
version: 1
top level: BBa_K394003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K394003/annotation2095519/range2095519/1

https://synbiohub.org/public/igem/BBa_K394003/annotation2095519/range2095519/1
type: Range
persistent identity: range2095519
display id: range2095519
version: 1
direction: direction/forward
top level: BBa_K394003
start: 1
end: 3
orientation: inline

BBa_K165100
type: ComponentDefinition
persistent identity: BBa K165100
display id: BBa_K165100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: true
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165031
component: BBa_K165003
component: BBa_K165004
component: BBa_K165012
component: BBa_K165009
component: BBa_K165018
component: BBa_K165002
sequence annotation: BBa_K165012
sequence annotation: BBa_K165002
sequence annotation: BBa_K165018
sequence annotation: BBa_K165004
sequence annotation: BBa_K165031
sequence annotation: BBa_K165009
sequence annotation: BBa_K165003
sequence: https://synbiohub.org/public/igem/BBa_K165100_sequence/1

BBa_K165031
type: Component
persistent identity: component1997398
display id: component1997398
version: 1
top level: BBa_K165100
access: public
definition: BBa_K165031

BBa_K165003
type: Component
persistent identity: component1997401
display id: component1997401
version: 1
top level: BBa_K165100
access: public
definition: Sin3

BBa_K165004
type: Component
persistent identity: component1997402
display id: component1997402
version: 1
top level: BBa_K165100
access: public
definition: mCherry

BBa_K165012
type: Component
persistent identity: component1997397
display id: component1997397
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165009
type: Component
persistent identity: component1997400
display id: component1997400
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165018
type: Component
persistent identity: component1997404
display id: component1997404
version: 1
top level: BBa_K165100
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165002
type: Component
persistent identity: component1997399
display id: component1997399
version: 1
top level: BBa_K165100
access: public
definition: BBa_K165002

BBa_K165004
type: Component
persistent identity: component1997403
display id: component1997403
version: 1
top level: BBa_K165100
access: public
definition: mCherry

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997397
display id: annotation1997397
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997397/range1997397/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165100/annotation1997397/range1997397/1
type: Range
persistent identity: range1997397
display id: range1997397
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 1
end: 127
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997399
display id: annotation1997399
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997399/range1997399/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165100/annotation1997399/range1997399/1
type: Range
persistent identity: range1997399
display id: range1997399
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 547
end: 564
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997404
display id: annotation1997404
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997404/range1997404/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165100/annotation1997404/range1997404/1
type: Range
persistent identity: range1997404
display id: range1997404
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 5144
end: 5396
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997402
display id: annotation1997402
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997402/range1997402/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165100/annotation1997402/range1997402/1
type: Range
persistent identity: range1997402
display id: range1997402
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 3658
end: 4392
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1997398
display id: annotation1997398
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997398/range1997398/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165100/annotation1997398/range1997398/1
type: Range
persistent identity: range1997398
display id: range1997398
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 136
end: 538
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997400
display id: annotation1997400
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997400/range1997400/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165100/annotation1997400/range1997400/1
type: Range
persistent identity: range1997400
display id: range1997400
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 573
end: 830
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997403
display id: annotation1997403
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997403/range1997403/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165100/annotation1997403/range1997403/1
type: Range
persistent identity: range1997403
display id: range1997403
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 4401
end: 5135
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997401
display id: annotation1997401
version: 1
top level: BBa_K165100
location: https://synbiohub.org/public/igem/BBa_K165100/annotation1997401/range1997401/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165100/annotation1997401/range1997401/1
type: Range
persistent identity: range1997401
display id: range1997401
version: 1
direction: direction/reverse
top level: BBa_K165100
start: 839
end: 3649
orientation: inline

BBa_K1680025
type: ComponentDefinition
persistent identity: BBa K1680025
display id: BBa_K1680025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-luciferase Cre reporter
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T03:54:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase.
mutable notes: RFC10 assembly
mutable provenance: designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1680005
component: BBa_K1680009
component: BBa_J63005
component: BBa_E1010
component: BBa_K392003
sequence annotation: BBa_J63005
sequence annotation: BBa_K1680009
sequence annotation: BBa_K1680005
sequence annotation: BBa_K392003
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1680025_sequence/1

BBa_K1680005
type: Component
persistent identity: component2466052
display id: component2466052
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K1680009
type: Component
persistent identity: component2466053
display id: component2466053
version: 1
top level: BBa_K1680025
access: public
definition: BBa_K1680009

BBa_J63005
type: Component
persistent identity: component2466046
display id: component2466046
version: 1
top level: BBa_K1680025
access: public
definition: P0310

BBa_E1010
type: Component
persistent identity: component2466050
display id: component2466050
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K1680005
type: Component
persistent identity: component2466047
display id: component2466047
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K392003
type: Component
persistent identity: component2466051
display id: component2466051
version: 1
top level: BBa_K1680025
access: public
definition: BBa_K392003

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2466046
display id: annotation2466046
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
type: Range
persistent identity: range2466046
display id: range2466046
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1
end: 1445
orientation: inline

BBa_K1680009
type: SequenceAnnotation
persistent identity: annotation2466053
display id: annotation2466053
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
component: BBa_K1680009

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
type: Range
persistent identity: range2466053
display id: range2466053
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2385
end: 2900
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466047
display id: annotation2466047
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
type: Range
persistent identity: range2466047
display id: range2466047
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1454
end: 1487
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466052
display id: annotation2466052
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
type: Range
persistent identity: range2466052
display id: range2466052
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2345
end: 2378
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2466051
display id: annotation2466051
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
type: Range
persistent identity: range2466051
display id: range2466051
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2208
end: 2336
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2466050
display id: annotation2466050
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
type: Range
persistent identity: range2466050
display id: range2466050
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1494
end: 2199
orientation: inline

BBa_K1861050
type: ComponentDefinition
persistent identity: BBa K1861050
display id: BBa_K1861050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of TALE1 and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861104
component: BBa_K1861501
component: BBa_K1861102
component: BBa_K1159201
component: BBa_K1861101
component: BBa_K392003
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861501
sequence annotation: BBa_K1861102
sequence: https://synbiohub.org/public/igem/BBa_K1861050_sequence/1

BBa_K1861104
type: Component
persistent identity: component2474417
display id: component2474417
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861501
type: Component
persistent identity: component2474413
display id: component2474413
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861501/1

BBa_K1861102
type: Component
persistent identity: component2474412
display id: component2474412
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1159201
type: Component
persistent identity: component2474416
display id: component2474416
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861101
type: Component
persistent identity: component2474411
display id: component2474411
version: 1
top level: BBa_K1861050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K392003
type: Component
persistent identity: component2474418
display id: component2474418
version: 1
top level: BBa_K1861050
access: public
definition: BBa_K392003

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474418
display id: annotation2474418
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474418/range2474418/1
type: Range
persistent identity: range2474418
display id: range2474418
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2525
end: 2653
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474416
display id: annotation2474416
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474416/range2474416/1
type: Range
persistent identity: range2474416
display id: range2474416
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2477
end: 2515
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474417
display id: annotation2474417
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474417/range2474417/1
type: Range
persistent identity: range2474417
display id: range2474417
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 2516
end: 2524
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474411
display id: annotation2474411
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474411/range2474411/1
type: Range
persistent identity: range2474411
display id: range2474411
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 1
end: 238
orientation: inline

BBa_K1861501
type: SequenceAnnotation
persistent identity: annotation2474413
display id: annotation2474413
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
component: BBa_K1861501

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474413/range2474413/1
type: Range
persistent identity: range2474413
display id: range2474413
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 266
end: 2476
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474412
display id: annotation2474412
version: 1
top level: BBa_K1861050
location: https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861050/annotation2474412/range2474412/1
type: Range
persistent identity: range2474412
display id: range2474412
version: 1
direction: direction/reverse
top level: BBa_K1861050
start: 239
end: 265
orientation: inline

BBa_K1680010
type: ComponentDefinition
persistent identity: BBa K1680010
display id: BBa_K1680010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:31Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the firefly [Photinus pyralis] luciferase, codon optimized for yeast.
mutable notes: Shipped in RFC 10
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1680010_sequence/1

Luciferase
type: SequenceAnnotation
persistent identity: annotation2477414
display id: annotation2477414
version: 1
top level: BBa_K1680010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680010/annotation2477414/range2477414/1

https://synbiohub.org/public/igem/BBa_K1680010/annotation2477414/range2477414/1
type: Range
persistent identity: range2477414
display id: range2477414
version: 1
direction: direction/forward
top level: BBa_K1680010
start: 1
end: 1653
orientation: inline

BBa_K1871002
type: ComponentDefinition
persistent identity: BBa K1871002
display id: BBa_K1871002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha amylase with mating factor alpha secretion
creator: Broad Run High School iGEM Team
created: 2015-09-12T11:00:00Z
modified: 2015-12-17T01:16:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2303_
m user id: 4206
owner id: 28667
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the alpha amylase from Bacillus amyloliquefaciens, flanked by Kozak sequence (Part BBa_K165002), mating factor alpha secretion sequence (Part BBa_K792002), and the ADH1 Terminator (Part BBa_K392003). This part is contained by the minimal cyc promoter (Part BBa_K105027).
mutable notes: TBD
mutable provenance: This is derived from the published sequence for Bacillus amyloliquefaciens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Alpha Amylase Coding Sequence
sequence annotation: Kozak Sequence
sequence annotation: Native Mating Factor Alpha Secretion Sequence
sequence annotation: ADH1 Terminator
sequence annotation: Minimal cyc Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1871002_sequence/1

Alpha Amylase Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2462573
display id: annotation2462573
version: 1
top level: BBa_K1871002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1871002/annotation2462573/range2462573/1

https://synbiohub.org/public/igem/BBa_K1871002/annotation2462573/range2462573/1
type: Range
persistent identity: range2462573
display id: range2462573
version: 1
direction: direction/forward
top level: BBa_K1871002
start: 180
end: 1631
orientation: inline

Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2462570
display id: annotation2462570
version: 1
top level: BBa_K1871002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1871002/annotation2462570/range2462570/1

https://synbiohub.org/public/igem/BBa_K1871002/annotation2462570/range2462570/1
type: Range
persistent identity: range2462570
display id: range2462570
version: 1
direction: direction/forward
top level: BBa_K1871002
start: 105
end: 125
orientation: inline

Native Mating Factor Alpha Secretion Sequence
type: SequenceAnnotation
persistent identity: annotation2462571
display id: annotation2462571
version: 1
top level: BBa_K1871002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1871002/annotation2462571/range2462571/1

https://synbiohub.org/public/igem/BBa_K1871002/annotation2462571/range2462571/1
type: Range
persistent identity: range2462571
display id: range2462571
version: 1
direction: direction/forward
top level: BBa_K1871002
start: 126
end: 179
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2462578
display id: annotation2462578
version: 1
top level: BBa_K1871002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1871002/annotation2462578/range2462578/1

https://synbiohub.org/public/igem/BBa_K1871002/annotation2462578/range2462578/1
type: Range
persistent identity: range2462578
display id: range2462578
version: 1
direction: direction/forward
top level: BBa_K1871002
start: 1632
end: 1819
orientation: inline

Minimal cyc Promoter
type: SequenceAnnotation
persistent identity: annotation2462569
display id: annotation2462569
version: 1
top level: BBa_K1871002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1871002/annotation2462569/range2462569/1

https://synbiohub.org/public/igem/BBa_K1871002/annotation2462569/range2462569/1
type: Range
persistent identity: range2462569
display id: range2462569
version: 1
direction: direction/forward
top level: BBa_K1871002
start: 1
end: 104
orientation: inline

Cyc1P Acce
type: ComponentDefinition
persistent identity: BBa K106014
display id: BBa_K106014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor vector for AarI based cloning. Accepts any combination of parts beginning with an A site and ending with a D site. Expression of construct is driven by a Cyc1 promoter, with Adh1 terminator.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS315 plasmid--CEN ARS yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: LEU2 marker
sequence annotation: Stop codons in all 3 frames
sequence annotation: ampicillin resistance marker
sequence annotation: ampicillin resistance promoter
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: lac promoter
sequence annotation: Start codon
sequence annotation: pBR322 origin
sequence annotation: Adh1 terminator
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106014_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999685
display id: annotation1999685
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1
type: Range
persistent identity: range1999685
display id: range1999685
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6394
end: 6397
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999688
display id: annotation1999688
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1
type: Range
persistent identity: range1999688
display id: range1999688
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999687
display id: annotation1999687
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1
type: Range
persistent identity: range1999687
display id: range1999687
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 246
end: 405
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986801
display id: annotation1986801
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1
type: Range
persistent identity: range1986801
display id: range1986801
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6132
end: 6394
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999692
display id: annotation1999692
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1
type: Range
persistent identity: range1999692
display id: range1999692
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6095
end: 6114
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986804
display id: annotation1986804
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1
type: Range
persistent identity: range1986804
display id: range1986804
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1570
end: 2676
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999703
display id: annotation1999703
version: 1
top level: Cyc1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1
type: Range
persistent identity: range1999703
display id: range1999703
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 11
end: 21
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986800
display id: annotation1986800
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1
type: Range
persistent identity: range1986800
display id: range1986800
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 4053
end: 4911
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999689
display id: annotation1999689
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1
type: Range
persistent identity: range1999689
display id: range1999689
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 3983
end: 4011
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999686
display id: annotation1999686
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1
type: Range
persistent identity: range1999686
display id: range1999686
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 224
end: 242
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1986802
display id: annotation1986802
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1
type: Range
persistent identity: range1986802
display id: range1986802
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1
end: 4
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999691
display id: annotation1999691
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1
type: Range
persistent identity: range1999691
display id: range1999691
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5996
end: 6025
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999704
display id: annotation1999704
version: 1
top level: Cyc1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1
type: Range
persistent identity: range1999704
display id: range1999704
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6385
end: 6387
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999690
display id: annotation1999690
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1
type: Range
persistent identity: range1999690
display id: range1999690
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5068
end: 5687
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986803
display id: annotation1986803
version: 1
top level: Cyc1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1
type: Range
persistent identity: range1986803
display id: range1986803
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 23
end: 216
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986799
display id: annotation1986799
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1
type: Range
persistent identity: range1986799
display id: range1986799
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 3403
end: 3921
orientation: inline

BBa_K165054
type: ComponentDefinition
persistent identity: BBa K165054
display id: BBa_K165054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full Gli1 Repressor (tagged with CFPx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+Gli1 DBD+CFPx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165018
component: BBa_K165014
component: BBa_K165007
component: BBa_K165003
component: BBa_K165002
sequence annotation: BBa_K165007
sequence annotation: BBa_K165014
sequence annotation: BBa_K165018
sequence annotation: BBa_K165003
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165054_sequence/1

BBa_K165018
type: Component
persistent identity: component1996739
display id: component1996739
version: 1
top level: BBa_K165054
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165014
type: Component
persistent identity: component1996738
display id: component1996738
version: 1
top level: BBa_K165054
access: public
definition: cfpx2

BBa_K165007
type: Component
persistent identity: component1996737
display id: component1996737
version: 1
top level: BBa_K165054
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165003
type: Component
persistent identity: component1996736
display id: component1996736
version: 1
top level: BBa_K165054
access: public
definition: Sin3

BBa_K165002
type: Component
persistent identity: component1996735
display id: component1996735
version: 1
top level: BBa_K165054
access: public
definition: BBa_K165002

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1996737
display id: annotation1996737
version: 1
top level: BBa_K165054
location: https://synbiohub.org/public/igem/BBa_K165054/annotation1996737/range1996737/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165054/annotation1996737/range1996737/1
type: Range
persistent identity: range1996737
display id: range1996737
version: 1
direction: direction/reverse
top level: BBa_K165054
start: 2846
end: 3370
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1996738
display id: annotation1996738
version: 1
top level: BBa_K165054
location: https://synbiohub.org/public/igem/BBa_K165054/annotation1996738/range1996738/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165054/annotation1996738/range1996738/1
type: Range
persistent identity: range1996738
display id: range1996738
version: 1
direction: direction/reverse
top level: BBa_K165054
start: 3379
end: 4872
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996739
display id: annotation1996739
version: 1
top level: BBa_K165054
location: https://synbiohub.org/public/igem/BBa_K165054/annotation1996739/range1996739/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165054/annotation1996739/range1996739/1
type: Range
persistent identity: range1996739
display id: range1996739
version: 1
direction: direction/reverse
top level: BBa_K165054
start: 4881
end: 5133
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996736
display id: annotation1996736
version: 1
top level: BBa_K165054
location: https://synbiohub.org/public/igem/BBa_K165054/annotation1996736/range1996736/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165054/annotation1996736/range1996736/1
type: Range
persistent identity: range1996736
display id: range1996736
version: 1
direction: direction/reverse
top level: BBa_K165054
start: 27
end: 2837
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996735
display id: annotation1996735
version: 1
top level: BBa_K165054
location: https://synbiohub.org/public/igem/BBa_K165054/annotation1996735/range1996735/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165054/annotation1996735/range1996735/1
type: Range
persistent identity: range1996735
display id: range1996735
version: 1
direction: direction/reverse
top level: BBa_K165054
start: 1
end: 18
orientation: inline

BBa_K792009
type: ComponentDefinition
persistent identity: BBa K792009
display id: BBa_K792009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast exportable His-rich peptide w/enhanced import (1)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). The high level structure of the device is: * Kozak consensus sequence for initiation of translation * Signal peptide that targets the product of the gene for secretion * Trojan peptide, to increase internalization in target cell * Payload: this is the exported amino acid rich domain of the protein
mutable notes: Composite part
mutable provenance: Composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K792002
component: BBa_K792001
component: BBa_K792003
component: BBa_K792005
sequence annotation: BBa_K792002
sequence annotation: BBa_K792001
sequence annotation: BBa_K792003
sequence annotation: BBa_K792005
sequence: https://synbiohub.org/public/igem/BBa_K792009_sequence/1

BBa_K792002
type: Component
persistent identity: component2196482
display id: component2196482
version: 1
top level: BBa_K792009
access: public
definition: MFa1-Secre

BBa_K792001
type: Component
persistent identity: component2196481
display id: component2196481
version: 1
top level: BBa_K792009
access: public
definition: MFa1-Kozak

BBa_K792003
type: Component
persistent identity: component2196484
display id: component2196484
version: 1
top level: BBa_K792009
access: public
definition: TAT-trojan

BBa_K792005
type: Component
persistent identity: component2196486
display id: component2196486
version: 1
top level: BBa_K792009
access: public
definition: HisTag

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2196482
display id: annotation2196482
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196482/range2196482/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K792009/annotation2196482/range2196482/1
type: Range
persistent identity: range2196482
display id: range2196482
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 22
end: 75
orientation: inline

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2196481
display id: annotation2196481
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196481/range2196481/1
component: BBa_K792001

https://synbiohub.org/public/igem/BBa_K792009/annotation2196481/range2196481/1
type: Range
persistent identity: range2196481
display id: range2196481
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 1
end: 21
orientation: inline

BBa_K792003
type: SequenceAnnotation
persistent identity: annotation2196484
display id: annotation2196484
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196484/range2196484/1
component: BBa_K792003

https://synbiohub.org/public/igem/BBa_K792009/annotation2196484/range2196484/1
type: Range
persistent identity: range2196484
display id: range2196484
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 76
end: 114
orientation: inline

BBa_K792005
type: SequenceAnnotation
persistent identity: annotation2196486
display id: annotation2196486
version: 1
top level: BBa_K792009
location: https://synbiohub.org/public/igem/BBa_K792009/annotation2196486/range2196486/1
component: BBa_K792005

https://synbiohub.org/public/igem/BBa_K792009/annotation2196486/range2196486/1
type: Range
persistent identity: range2196486
display id: range2196486
version: 1
direction: direction/reverse
top level: BBa_K792009
start: 115
end: 162
orientation: inline

BBa_K165076
type: ComponentDefinition
persistent identity: BBa K165076
display id: BBa_K165076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMET25 + YY1 repressor on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K165076_sequence/1

BBa_I766214
type: ComponentDefinition
persistent identity: BBa I766214
display id: BBa_I766214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGal1
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pGAl1 promoter region
mutable notes: none
mutable provenance: yeast
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766214_sequence/1

BBa_K1907004
type: ComponentDefinition
persistent identity: BBa K1907004
display id: BBa_K1907004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TSA1 promoter for S. cerevisiae
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T03:20:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1907004_sequence/1

ECFP
type: ComponentDefinition
persistent identity: BBa K105009
display id: BBa_K105009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP, yeast optimized for fusion proteins
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is cyan fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of BioBrick part for fusion proteins (see design details).
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of the ECFP coding part of BBa_E2020 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105009_sequence/1

P0310
type: ComponentDefinition
persistent identity: BBa J63005
display id: BBa_J63005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 promoter from S. cerevisiae
mutable notes: no changes to genomic sequence
mutable provenance: genomic DNA of ADH1 from S. cerevisiae
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA Box
sequence annotation: Constitutive promoter
sequence: https://synbiohub.org/public/igem/BBa_J63005_sequence/1

TATA Box
type: SequenceAnnotation
persistent identity: annotation1902745
display id: annotation1902745
version: 1
top level: P0310
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1
type: Range
persistent identity: range1902745
display id: range1902745
version: 1
direction: direction/forward
top level: P0310
start: 1348
end: 1354
orientation: inline

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation1902744
display id: annotation1902744
version: 1
top level: P0310
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1
type: Range
persistent identity: range1902744
display id: range1902744
version: 1
direction: direction/forward
top level: P0310
start: 1
end: 1445
orientation: inline

tADH1
type: ComponentDefinition
persistent identity: BBa J63002
display id: BBa_J63002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 terminator from S. cerevisiae
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 terminator from S. cerevisiae
mutable notes: starts with stop codon
mutable provenance: genomic DNA of S. cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J63002_sequence/1

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation1902851
display id: annotation1902851
version: 1
top level: tADH1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1

https://synbiohub.org/public/igem/BBa_J63002/annotation1902851/range1902851/1
type: Range
persistent identity: range1902851
display id: range1902851
version: 1
direction: direction/forward
top level: tADH1
start: 1
end: 225
orientation: inline

BBa_K1486034
type: ComponentDefinition
persistent identity: BBa K1486034
display id: BBa_K1486034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP C-terminus (215-238)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Yeast optimized sfGFP C-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486034_sequence/1

Yeast optimized sfGFP C-terminus
type: SequenceAnnotation
persistent identity: annotation2417784
display id: annotation2417784
version: 1
top level: BBa_K1486034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1

https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1
type: Range
persistent identity: range2417784
display id: range2417784
version: 1
direction: direction/forward
top level: BBa_K1486034
start: 1
end: 75
orientation: inline

BBa_K106011
type: ComponentDefinition
persistent identity: BBa K106011
display id: BBa_K106011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sas2 histone acetyltransferase, Aar1 AB part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding for the Sas2 gene in yeast. It is a histone acetyltransferase (HAT).
mutable notes: Since this is an AB part, there is a ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106011_sequence/1

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 yeast terminator
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ADH1 terminator of ''S. cerevisiae''
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
direction: direction/forward
top level: BBa_K801012
start: 1
end: 349
orientation: inline

BBa_K165099
type: ComponentDefinition
persistent identity: BBa K165099
display id: BBa_K165099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + ADH1+ LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165003
component: BBa_K165014
component: BBa_K165009
component: BBa_K165002
component: BBa_K165012
component: BBa_K165018
component: BBa_K165015
sequence annotation: BBa_K165009
sequence annotation: BBa_K165014
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165099_sequence/1

BBa_K165003
type: Component
persistent identity: component1997394
display id: component1997394
version: 1
top level: BBa_K165099
access: public
definition: Sin3

BBa_K165014
type: Component
persistent identity: component1997395
display id: component1997395
version: 1
top level: BBa_K165099
access: public
definition: cfpx2

BBa_K165009
type: Component
persistent identity: component1997393
display id: component1997393
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165002
type: Component
persistent identity: component1997392
display id: component1997392
version: 1
top level: BBa_K165099
access: public
definition: BBa_K165002

BBa_K165012
type: Component
persistent identity: component1997390
display id: component1997390
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165018
type: Component
persistent identity: component1997396
display id: component1997396
version: 1
top level: BBa_K165099
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165015
type: Component
persistent identity: component1997391
display id: component1997391
version: 1
top level: BBa_K165099
access: public
definition: BBa_K165015

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997393
display id: annotation1997393
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997393/range1997393/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165099/annotation1997393/range1997393/1
type: Range
persistent identity: range1997393
display id: range1997393
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1615
end: 1872
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997395
display id: annotation1997395
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997395/range1997395/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165099/annotation1997395/range1997395/1
type: Range
persistent identity: range1997395
display id: range1997395
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 4700
end: 6193
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997392
display id: annotation1997392
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997392/range1997392/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165099/annotation1997392/range1997392/1
type: Range
persistent identity: range1997392
display id: range1997392
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1589
end: 1606
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997394
display id: annotation1997394
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997394/range1997394/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165099/annotation1997394/range1997394/1
type: Range
persistent identity: range1997394
display id: range1997394
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1881
end: 4691
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997396
display id: annotation1997396
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997396/range1997396/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165099/annotation1997396/range1997396/1
type: Range
persistent identity: range1997396
display id: range1997396
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 6202
end: 6454
orientation: inline

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1997391
display id: annotation1997391
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997391/range1997391/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165099/annotation1997391/range1997391/1
type: Range
persistent identity: range1997391
display id: range1997391
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997390
display id: annotation1997390
version: 1
top level: BBa_K165099
location: https://synbiohub.org/public/igem/BBa_K165099/annotation1997390/range1997390/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165099/annotation1997390/range1997390/1
type: Range
persistent identity: range1997390
display id: range1997390
version: 1
direction: direction/reverse
top level: BBa_K165099
start: 1
end: 127
orientation: inline

BBa_K106670
type: ComponentDefinition
persistent identity: BBa K106670
display id: BBa_K106670
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor, pRS315, 8X LexAOPs Cyc1P, Adht1
creator: Andrew Horwitz
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning with 8X Lex operators directly upstream of a Cyc1 promoter. The vector contains the AarI cloning cassette (AD sites, with Adh1 terminator). It is a pRS315 derived vector, for CEN ARS expression in cerevisiae (LEU2 marker).
mutable notes: LexA operator repeats were cloned into the PspOMI site at the 5' end of the Cyc1 promoter. The inner site was broken, thus the operator-promoter-AarI cassette fragment can be subcloned by PspOMI/SacI or Kpn1/SacI digestion into any other pRS series vector.
mutable provenance: This is a derivative of part BBa_K106014, the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ATG start codon
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: ampicillin resistance marker
sequence annotation: lac promoter
sequence annotation: pBR322 origin
sequence annotation: T7 promoter
sequence annotation: Adh1 terminator
sequence annotation: LEU2 Marker
sequence annotation: 8X LexA Operators
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: A overhang
sequence annotation: ampicillin resistance promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: lacZalpha reporter
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106670_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999702
display id: annotation1999702
version: 1
top level: BBa_K106670
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1
type: Range
persistent identity: range1999702
display id: range1999702
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6547
end: 6549
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1990434
display id: annotation1990434
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1
type: Range
persistent identity: range1990434
display id: range1990434
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6300
end: 6556
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999701
display id: annotation1999701
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1
type: Range
persistent identity: range1999701
display id: range1999701
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6095
end: 6114
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1990432
display id: annotation1990432
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1
type: Range
persistent identity: range1990432
display id: range1990432
version: 1
direction: direction/forward
top level: BBa_K106670
start: 4053
end: 4911
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999700
display id: annotation1999700
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1
type: Range
persistent identity: range1999700
display id: range1999700
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5996
end: 6025
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999699
display id: annotation1999699
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1
type: Range
persistent identity: range1999699
display id: range1999699
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5068
end: 5687
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999696
display id: annotation1999696
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1
type: Range
persistent identity: range1999696
display id: range1999696
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 224
end: 242
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1990437
display id: annotation1990437
version: 1
top level: BBa_K106670
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1
type: Range
persistent identity: range1990437
display id: range1990437
version: 1
direction: direction/forward
top level: BBa_K106670
start: 23
end: 216
orientation: inline

LEU2 Marker
type: SequenceAnnotation
persistent identity: annotation1990438
display id: annotation1990438
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1
type: Range
persistent identity: range1990438
display id: range1990438
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1570
end: 2676
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1990433
display id: annotation1990433
version: 1
top level: BBa_K106670
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1
type: Range
persistent identity: range1990433
display id: range1990433
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6132
end: 6299
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999698
display id: annotation1999698
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1
type: Range
persistent identity: range1999698
display id: range1999698
version: 1
direction: direction/forward
top level: BBa_K106670
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999693
display id: annotation1999693
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1
type: Range
persistent identity: range1999693
display id: range1999693
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1
end: 4
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999695
display id: annotation1999695
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1
type: Range
persistent identity: range1999695
display id: range1999695
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6556
end: 6559
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1990435
display id: annotation1990435
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1
type: Range
persistent identity: range1990435
display id: range1990435
version: 1
direction: direction/forward
top level: BBa_K106670
start: 3983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999694
display id: annotation1999694
version: 1
top level: BBa_K106670
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1
type: Range
persistent identity: range1999694
display id: range1999694
version: 1
direction: direction/forward
top level: BBa_K106670
start: 11
end: 21
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999697
display id: annotation1999697
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1
type: Range
persistent identity: range1999697
display id: range1999697
version: 1
direction: direction/forward
top level: BBa_K106670
start: 246
end: 405
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1990431
display id: annotation1990431
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1
type: Range
persistent identity: range1990431
display id: range1990431
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 3403
end: 3921
orientation: inline

BBa_K1680012
type: ComponentDefinition
persistent identity: BBa K1680012
display id: BBa_K1680012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cleavage site for HCV protease
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:43:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the cleavage site of the HCV NS3-4A protease (Part BBa_K1680011).
mutable notes: Shipped in RFC 25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1680012_sequence/1

BBa_K105028
type: ComponentDefinition
persistent identity: BBa K105028
display id: BBa_K105028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc70 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A->C
sequence: https://synbiohub.org/public/igem/BBa_K105028_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989835
display id: annotation1989835
version: 1
top level: BBa_K105028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1
type: Range
persistent identity: range1989835
display id: range1989835
version: 1
direction: direction/forward
top level: BBa_K105028
start: 17
end: 23
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1989836
display id: annotation1989836
version: 1
top level: BBa_K105028
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1
type: Range
persistent identity: range1989836
display id: range1989836
version: 1
direction: direction/forward
top level: BBa_K105028
start: 24
end: 24
orientation: inline

BBa_K950000
type: ComponentDefinition
persistent identity: BBa K950000
display id: BBa_K950000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast fet3 promotor
creator: Jakob Matthes
created: 2012-07-09T11:00:00Z
modified: 2015-09-25T08:36:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1218_
m user id: 26366
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: promotor of the fet3 gene from S. cerevisiae, fet3 is repressed on high iron levels.
mutable notes: length of promoter was chosen to include binding sites and to obtain good primers
mutable provenance: genomic upstream sequence of fet3 gene from the Saccharomyces Genome Database (http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=fet3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: binding site
sequence: https://synbiohub.org/public/igem/BBa_K950000_sequence/1

binding site
type: SequenceAnnotation
persistent identity: annotation2182746
display id: annotation2182746
version: 1
top level: BBa_K950000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1

https://synbiohub.org/public/igem/BBa_K950000/annotation2182746/range2182746/1
type: Range
persistent identity: range2182746
display id: range2182746
version: 1
direction: direction/forward
top level: BBa_K950000
start: 264
end: 268
orientation: inline

BBa_K1611001
type: ComponentDefinition
persistent identity: BBa K1611001
display id: BBa_K1611001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MATalpha-IFNgamma
creator: Frederic Ros
created: 2015-09-14T11:00:00Z
modified: 2015-12-17T01:03:54Z
discontinued: false
dominant: false
group u list: _2028_
m user id: 4206
owner id: 28291
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.
mutable notes: We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.
mutable provenance: MAT alpha was amplified from the S. cerevisiae genome. IFN gamma was synthesised by IDT based on the genomic murine IFN gamma. Both sequences have been assembled by golden gate to make a single fusion protein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: c.462G>C
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1611001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2456823
display id: annotation2456823
version: 1
top level: BBa_K1611001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1611001/annotation2456823/range2456823/1

https://synbiohub.org/public/igem/BBa_K1611001/annotation2456823/range2456823/1
type: Range
persistent identity: range2456823
display id: range2456823
version: 1
direction: direction/forward
top level: BBa_K1611001
start: 1
end: 3
orientation: inline

c.462G>C
type: SequenceAnnotation
persistent identity: annotation2456825
display id: annotation2456825
version: 1
top level: BBa_K1611001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1611001/annotation2456825/range2456825/1

https://synbiohub.org/public/igem/BBa_K1611001/annotation2456825/range2456825/1
type: Range
persistent identity: range2456825
display id: range2456825
version: 1
direction: direction/forward
top level: BBa_K1611001
start: 462
end: 462
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2456824
display id: annotation2456824
version: 1
top level: BBa_K1611001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1611001/annotation2456824/range2456824/1

https://synbiohub.org/public/igem/BBa_K1611001/annotation2456824/range2456824/1
type: Range
persistent identity: range2456824
display id: range2456824
version: 1
direction: direction/forward
top level: BBa_K1611001
start: 1
end: 732
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2456826
display id: annotation2456826
version: 1
top level: BBa_K1611001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1611001/annotation2456826/range2456826/1

https://synbiohub.org/public/igem/BBa_K1611001/annotation2456826/range2456826/1
type: Range
persistent identity: range2456826
display id: range2456826
version: 1
direction: direction/forward
top level: BBa_K1611001
start: 730
end: 735
orientation: inline

BBa_K110012
type: ComponentDefinition
persistent identity: BBa K110012
display id: BBa_K110012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: STE2 terminator
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
mutable notes: This sequence was taken from the yeast genome
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Terminator
sequence: https://synbiohub.org/public/igem/BBa_K110012_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation1994877
display id: annotation1994877
version: 1
top level: BBa_K110012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1

https://synbiohub.org/public/igem/BBa_K110012/annotation1994877/range1994877/1
type: Range
persistent identity: range1994877
display id: range1994877
version: 1
direction: direction/forward
top level: BBa_K110012
start: 1
end: 123
orientation: inline

BBa_K1680009
type: ComponentDefinition
persistent identity: BBa K1680009
display id: BBa_K1680009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the NanoLuc(tm) Luziferase (designed by the Promega Corporation, free for research use), Codon optimized for yeast. The Nanoluc is smaller than other luziferase proteins and yields equal or stronger luminescence readings.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen, sequence by Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc
sequence: https://synbiohub.org/public/igem/BBa_K1680009_sequence/1

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2477413
display id: annotation2477413
version: 1
top level: BBa_K1680009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1

https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1
type: Range
persistent identity: range2477413
display id: range2477413
version: 1
direction: direction/forward
top level: BBa_K1680009
start: 1
end: 516
orientation: inline

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

BBa_K801010
type: ComponentDefinition
persistent identity: BBa K801010
display id: BBa_K801010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 constitutive yeast promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive yeast promoter of medium strength compared to TEF1 promoter.
mutable notes: -
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TEF1-Promoter
sequence: https://synbiohub.org/public/igem/BBa_K801010_sequence/1

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2202139
display id: annotation2202139
version: 1
top level: BBa_K801010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1

https://synbiohub.org/public/igem/BBa_K801010/annotation2202139/range2202139/1
type: Range
persistent identity: range2202139
display id: range2202139
version: 1
direction: direction/forward
top level: BBa_K801010
start: 1
end: 647
orientation: inline

BBa_K165079
type: ComponentDefinition
persistent identity: BBa K165079
display id: BBa_K165079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Null synthetic activator on pRS305
creator: Aaron Glieberman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Note that this is a ligation that we made onto a pRS305 vector, which does not conform to Biobrick standard. As such, there is some remnant of the Biobrick ends but
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165046
component: BBa_K165029
sequence annotation: BBa_K165046
sequence annotation: BBa_K165029
sequence: https://synbiohub.org/public/igem/BBa_K165079_sequence/1

BBa_K165046
type: Component
persistent identity: component1997119
display id: component1997119
version: 1
top level: BBa_K165079
access: public
definition: BBa_K165046

BBa_K165029
type: Component
persistent identity: component1997120
display id: component1997120
version: 1
top level: BBa_K165079
access: public
definition: https://synbiohub.org/public/igem/BBa_K165029/1

BBa_K165046
type: SequenceAnnotation
persistent identity: annotation1997119
display id: annotation1997119
version: 1
top level: BBa_K165079
location: https://synbiohub.org/public/igem/BBa_K165079/annotation1997119/range1997119/1
component: BBa_K165046

https://synbiohub.org/public/igem/BBa_K165079/annotation1997119/range1997119/1
type: Range
persistent identity: range1997119
display id: range1997119
version: 1
direction: direction/reverse
top level: BBa_K165079
start: 1
end: 549
orientation: inline

BBa_K165029
type: SequenceAnnotation
persistent identity: annotation1997120
display id: annotation1997120
version: 1
top level: BBa_K165079
location: https://synbiohub.org/public/igem/BBa_K165079/annotation1997120/range1997120/1
component: BBa_K165029

https://synbiohub.org/public/igem/BBa_K165079/annotation1997120/range1997120/1
type: Range
persistent identity: range1997120
display id: range1997120
version: 1
direction: direction/reverse
top level: BBa_K165079
start: 558
end: 2447
orientation: inline

BBa_K110004
type: ComponentDefinition
persistent identity: BBa K110004
display id: BBa_K110004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter Ste3
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This sequence was not edited
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110004_sequence/1

BBa_K801074
type: ComponentDefinition
persistent identity: BBa K801074
display id: BBa_K801074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaMXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yeast expression cassette for CaMXMT1 (BBa_K801071) controlled by the yeast TEF1 promoter (BBa_K319003) and the yeast ADH1 terminator (BBa_K801012).
mutable notes: -
mutable provenance: Composite part of BBa_K319003, BBa_K801071 and BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Consensus sequence
sequence annotation: BioBrick
sequence annotation: Stop codon
sequence annotation: TEF1 promoter
sequence annotation: CaMXMT1
sequence annotation: Strep-Tag
sequence annotation: Start codon
sequence annotation: ADH1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801074_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199499
display id: annotation2199499
version: 1
top level: BBa_K801074
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199499/range2199499/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199499/range2199499/1
type: Range
persistent identity: range2199499
display id: range2199499
version: 1
direction: direction/forward
top level: BBa_K801074
start: 458
end: 463
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation2197320
display id: annotation2197320
version: 1
top level: BBa_K801074
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197320/range2197320/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197320/range2197320/1
type: Range
persistent identity: range2197320
display id: range2197320
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1
end: 1991
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199500
display id: annotation2199500
version: 1
top level: BBa_K801074
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199500/range2199500/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199500/range2199500/1
type: Range
persistent identity: range2199500
display id: range2199500
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1628
end: 1633
orientation: inline

TEF1 promoter
type: SequenceAnnotation
persistent identity: annotation2197321
display id: annotation2197321
version: 1
top level: BBa_K801074
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197321/range2197321/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197321/range2197321/1
type: Range
persistent identity: range2197321
display id: range2197321
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1
end: 450
orientation: inline

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197323
display id: annotation2197323
version: 1
top level: BBa_K801074
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197323/range2197323/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197323/range2197323/1
type: Range
persistent identity: range2197323
display id: range2197323
version: 1
direction: direction/forward
top level: BBa_K801074
start: 464
end: 1597
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199502
display id: annotation2199502
version: 1
top level: BBa_K801074
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199502/range2199502/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199502/range2199502/1
type: Range
persistent identity: range2199502
display id: range2199502
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1598
end: 1627
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201346
display id: annotation2201346
version: 1
top level: BBa_K801074
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2201346/range2201346/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2201346/range2201346/1
type: Range
persistent identity: range2201346
display id: range2201346
version: 1
direction: direction/forward
top level: BBa_K801074
start: 464
end: 466
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197327
display id: annotation2197327
version: 1
top level: BBa_K801074
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197327/range2197327/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197327/range2197327/1
type: Range
persistent identity: range2197327
display id: range2197327
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1642
end: 1991
orientation: inline

BBa_K801061
type: ComponentDefinition
persistent identity: BBa K801061
display id: BBa_K801061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-Limonene synthase 1 coding region
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: (+)-Limonene synthase 1 coding region of ''Citrus limon''. Improved version of BBa_I742111.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/21435702
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801061_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197239
display id: annotation2197239
version: 1
top level: BBa_K801061
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801061/annotation2197239/range2197239/1

https://synbiohub.org/public/igem/BBa_K801061/annotation2197239/range2197239/1
type: Range
persistent identity: range2197239
display id: range2197239
version: 1
direction: direction/forward
top level: BBa_K801061
start: 1
end: 1665
orientation: inline

BBa_K165058
type: ComponentDefinition
persistent identity: BBa K165058
display id: BBa_K165058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak + YFP
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K165005
component: BBa_K165002
sequence annotation: BBa_K165002
sequence annotation: BBa_K165005
sequence: https://synbiohub.org/public/igem/BBa_K165058_sequence/1

BBa_K165005
type: Component
persistent identity: component1997049
display id: component1997049
version: 1
top level: BBa_K165058
access: public
definition: yfp

BBa_K165002
type: Component
persistent identity: component1997048
display id: component1997048
version: 1
top level: BBa_K165058
access: public
definition: BBa_K165002

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997048
display id: annotation1997048
version: 1
top level: BBa_K165058
location: https://synbiohub.org/public/igem/BBa_K165058/annotation1997048/range1997048/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165058/annotation1997048/range1997048/1
type: Range
persistent identity: range1997048
display id: range1997048
version: 1
direction: direction/reverse
top level: BBa_K165058
start: 1
end: 18
orientation: inline

BBa_K165005
type: SequenceAnnotation
persistent identity: annotation1997049
display id: annotation1997049
version: 1
top level: BBa_K165058
location: https://synbiohub.org/public/igem/BBa_K165058/annotation1997049/range1997049/1
component: BBa_K165005

https://synbiohub.org/public/igem/BBa_K165058/annotation1997049/range1997049/1
type: Range
persistent identity: range1997049
display id: range1997049
version: 1
direction: direction/reverse
top level: BBa_K165058
start: 27
end: 770
orientation: inline

BBa_K1486033
type: ComponentDefinition
persistent identity: BBa K1486033
display id: BBa_K1486033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + CaUra3
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486020
component: BBa_K1486031
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486019
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486025
sequence: https://synbiohub.org/public/igem/BBa_K1486033_sequence/1

BBa_K1486020
type: Component
persistent identity: component2418784
display id: component2418784
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486031
type: Component
persistent identity: component2418790
display id: component2418790
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486031

BBa_K1486025
type: Component
persistent identity: component2418786
display id: component2418786
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2418782
display id: component2418782
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486019
type: Component
persistent identity: component2418783
display id: component2418783
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2418783
display id: annotation2418783
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
type: Range
persistent identity: range2418783
display id: range2418783
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 40
end: 369
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418790
display id: annotation2418790
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
type: Range
persistent identity: range2418790
display id: range2418790
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1164
end: 2523
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418782
display id: annotation2418782
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
type: Range
persistent identity: range2418782
display id: range2418782
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1
end: 39
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418784
display id: annotation2418784
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
type: Range
persistent identity: range2418784
display id: range2418784
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 370
end: 975
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418786
display id: annotation2418786
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
type: Range
persistent identity: range2418786
display id: range2418786
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 976
end: 1163
orientation: inline

BBa_K165031
type: ComponentDefinition
persistent identity: BBa K165031
display id: BBa_K165031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus LexA binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
mutable provenance: Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165031_sequence/1

BBa_K165094
type: ComponentDefinition
persistent identity: BBa K165094
display id: BBa_K165094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + TEF2 + LexA repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165012
component: BBa_K165037
component: BBa_K165018
component: BBa_K165009
component: BBa_K165002
component: BBa_K165014
component: BBa_K165003
sequence annotation: BBa_K165009
sequence annotation: BBa_K165012
sequence annotation: BBa_K165037
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence annotation: BBa_K165003
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165094_sequence/1

BBa_K165012
type: Component
persistent identity: component1997335
display id: component1997335
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165037
type: Component
persistent identity: component1997336
display id: component1997336
version: 1
top level: BBa_K165094
access: public
definition: pTEF

BBa_K165018
type: Component
persistent identity: component1997341
display id: component1997341
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165009
type: Component
persistent identity: component1997338
display id: component1997338
version: 1
top level: BBa_K165094
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165002
type: Component
persistent identity: component1997337
display id: component1997337
version: 1
top level: BBa_K165094
access: public
definition: BBa_K165002

BBa_K165014
type: Component
persistent identity: component1997340
display id: component1997340
version: 1
top level: BBa_K165094
access: public
definition: cfpx2

BBa_K165003
type: Component
persistent identity: component1997339
display id: component1997339
version: 1
top level: BBa_K165094
access: public
definition: Sin3

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1997338
display id: annotation1997338
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997338/range1997338/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165094/annotation1997338/range1997338/1
type: Range
persistent identity: range1997338
display id: range1997338
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 573
end: 830
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997335
display id: annotation1997335
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997335/range1997335/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165094/annotation1997335/range1997335/1
type: Range
persistent identity: range1997335
display id: range1997335
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 1
end: 127
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997336
display id: annotation1997336
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997336/range1997336/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165094/annotation1997336/range1997336/1
type: Range
persistent identity: range1997336
display id: range1997336
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 136
end: 538
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997341
display id: annotation1997341
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997341/range1997341/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165094/annotation1997341/range1997341/1
type: Range
persistent identity: range1997341
display id: range1997341
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 5160
end: 5412
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997337
display id: annotation1997337
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997337/range1997337/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165094/annotation1997337/range1997337/1
type: Range
persistent identity: range1997337
display id: range1997337
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 547
end: 564
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997339
display id: annotation1997339
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997339/range1997339/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165094/annotation1997339/range1997339/1
type: Range
persistent identity: range1997339
display id: range1997339
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 839
end: 3649
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997340
display id: annotation1997340
version: 1
top level: BBa_K165094
location: https://synbiohub.org/public/igem/BBa_K165094/annotation1997340/range1997340/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165094/annotation1997340/range1997340/1
type: Range
persistent identity: range1997340
display id: range1997340
version: 1
direction: direction/reverse
top level: BBa_K165094
start: 3658
end: 5151
orientation: inline

BBa_K1861020
type: ComponentDefinition
persistent identity: BBa K1861020
display id: BBa_K1861020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of the TALE constant domain and a SpyTag in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T05:05:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1861104
component: BBa_K1861201
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1159201
component: BBa_K1861102
sequence annotation: BBa_K1861104
sequence annotation: BBa_K1861201
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1159201
sequence annotation: BBa_K1861101
sequence: https://synbiohub.org/public/igem/BBa_K1861020_sequence/1

BBa_K1861104
type: Component
persistent identity: component2474346
display id: component2474346
version: 1
top level: BBa_K1861020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861201
type: Component
persistent identity: component2474342
display id: component2474342
version: 1
top level: BBa_K1861020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861201/1

BBa_K392003
type: Component
persistent identity: component2474347
display id: component2474347
version: 1
top level: BBa_K1861020
access: public
definition: BBa_K392003

BBa_K1861101
type: Component
persistent identity: component2474340
display id: component2474340
version: 1
top level: BBa_K1861020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1159201
type: Component
persistent identity: component2474345
display id: component2474345
version: 1
top level: BBa_K1861020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159201/1

BBa_K1861102
type: Component
persistent identity: component2474341
display id: component2474341
version: 1
top level: BBa_K1861020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474346
display id: annotation2474346
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474346/range2474346/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474346/range2474346/1
type: Range
persistent identity: range2474346
display id: range2474346
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 1331
end: 1339
orientation: inline

BBa_K1861201
type: SequenceAnnotation
persistent identity: annotation2474342
display id: annotation2474342
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474342/range2474342/1
component: BBa_K1861201

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474342/range2474342/1
type: Range
persistent identity: range2474342
display id: range2474342
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 266
end: 1291
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474347
display id: annotation2474347
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474347/range2474347/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474347/range2474347/1
type: Range
persistent identity: range2474347
display id: range2474347
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 1340
end: 1468
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474341
display id: annotation2474341
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474341/range2474341/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474341/range2474341/1
type: Range
persistent identity: range2474341
display id: range2474341
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 239
end: 265
orientation: inline

BBa_K1159201
type: SequenceAnnotation
persistent identity: annotation2474345
display id: annotation2474345
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474345/range2474345/1
component: BBa_K1159201

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474345/range2474345/1
type: Range
persistent identity: range2474345
display id: range2474345
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 1292
end: 1330
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474340
display id: annotation2474340
version: 1
top level: BBa_K1861020
location: https://synbiohub.org/public/igem/BBa_K1861020/annotation2474340/range2474340/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861020/annotation2474340/range2474340/1
type: Range
persistent identity: range2474340
display id: range2474340
version: 1
direction: direction/reverse
top level: BBa_K1861020
start: 1
end: 238
orientation: inline

BBa_K1936000
type: ComponentDefinition
persistent identity: BBa K1936000
display id: BBa_K1936000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ePDZ-mCherry-BAX184E
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker
sequence annotation: mCherry
sequence annotation: ePDZ
sequence annotation: BaxS184e
sequence: https://synbiohub.org/public/igem/BBa_K1936000_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2521532
display id: annotation2521532
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521532/range2521532/1
type: Range
persistent identity: range2521532
display id: range2521532
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 583
end: 609
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521535
display id: annotation2521535
version: 1
top level: BBa_K1936000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521535/range2521535/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521535/range2521535/1
type: Range
persistent identity: range2521535
display id: range2521535
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1318
end: 1344
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2521534
display id: annotation2521534
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521534/range2521534/1
type: Range
persistent identity: range2521534
display id: range2521534
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 610
end: 1317
orientation: inline

ePDZ
type: SequenceAnnotation
persistent identity: annotation2521533
display id: annotation2521533
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521533/range2521533/1
type: Range
persistent identity: range2521533
display id: range2521533
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1
end: 582
orientation: inline

BaxS184e
type: SequenceAnnotation
persistent identity: annotation2521536
display id: annotation2521536
version: 1
top level: BBa_K1936000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1

https://synbiohub.org/public/igem/BBa_K1936000/annotation2521536/range2521536/1
type: Range
persistent identity: range2521536
display id: range2521536
version: 1
direction: direction/forward
top level: BBa_K1936000
start: 1345
end: 1920
orientation: inline

BBa_K1680004
type: ComponentDefinition
persistent identity: BBa K1680004
display id: BBa_K1680004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 NLS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:41:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for nuclear localization signal of SV40 large T antigen (amino acids: PKKKRKV).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1680004_sequence/1

Venus YFP
type: ComponentDefinition
persistent identity: BBa J63001
display id: BBa_J63001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of EYFP, yeast-optimized YFP
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized fast folding variant of YFP
mutable notes: as published
mutable provenance: Thorn et al
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Venus YFP
sequence: https://synbiohub.org/public/igem/BBa_J63001_sequence/1

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1902850
display id: annotation1902850
version: 1
top level: Venus YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63001/annotation1902850/range1902850/1

https://synbiohub.org/public/igem/BBa_J63001/annotation1902850/range1902850/1
type: Range
persistent identity: range1902850
display id: range1902850
version: 1
direction: direction/forward
top level: Venus YFP
start: 1
end: 744
orientation: inline

Sir4
type: ComponentDefinition
persistent identity: BBa K106001
display id: BBa_K106001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sir4, Aar1 AD part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for the SSn8 gene in yeast. Ssn8 is involved in phosphorylation of the RNA polymerase C-terminal. It is involved in glucose repression as well as telomere maintenance. In our project Ssn8 is used as a "modifier" to enhance silencing.
mutable notes: None
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106001_sequence/1

BBa_K775003
type: ComponentDefinition
persistent identity: BBa K775003
display id: BBa_K775003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator
creator: Sietske Grijseels
created: 2012-09-04T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1027_
m user id: 0
owner id: 12035
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7.
mutable notes: -
mutable provenance: N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K775003_sequence/1

BBa_K801002
type: ComponentDefinition
persistent identity: BBa K801002
display id: BBa_K801002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM102 yeast shuttle vector with pTEF2 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: TEF2-Promoter
sequence annotation: T7-Promoter
sequence annotation: 2 micron (??) origin
sequence annotation: CYC1-T
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence annotation: URA3
sequence: https://synbiohub.org/public/igem/BBa_K801002_sequence/1

TEF2-Promoter
type: SequenceAnnotation
persistent identity: annotation2201314
display id: annotation2201314
version: 1
top level: BBa_K801002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201314/range2201314/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201314/range2201314/1
type: Range
persistent identity: range2201314
display id: range2201314
version: 1
direction: direction/forward
top level: BBa_K801002
start: 58
end: 705
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201456
display id: annotation2201456
version: 1
top level: BBa_K801002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201456/range2201456/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201456/range2201456/1
type: Range
persistent identity: range2201456
display id: range2201456
version: 1
direction: direction/forward
top level: BBa_K801002
start: 4
end: 23
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201452
display id: annotation2201452
version: 1
top level: BBa_K801002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201452/range2201452/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201452/range2201452/1
type: Range
persistent identity: range2201452
display id: range2201452
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 3976
end: 5447
orientation: inline

CYC1-T
type: SequenceAnnotation
persistent identity: annotation2201315
display id: annotation2201315
version: 1
top level: BBa_K801002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201315/range2201315/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201315/range2201315/1
type: Range
persistent identity: range2201315
display id: range2201315
version: 1
direction: direction/forward
top level: BBa_K801002
start: 739
end: 984
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201446
display id: annotation2201446
version: 1
top level: BBa_K801002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201446/range2201446/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201446/range2201446/1
type: Range
persistent identity: range2201446
display id: range2201446
version: 1
direction: direction/forward
top level: BBa_K801002
start: 1169
end: 1842
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201447
display id: annotation2201447
version: 1
top level: BBa_K801002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201447/range2201447/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201447/range2201447/1
type: Range
persistent identity: range2201447
display id: range2201447
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 1987
end: 2846
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201448
display id: annotation2201448
version: 1
top level: BBa_K801002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201448/range2201448/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201448/range2201448/1
type: Range
persistent identity: range2201448
display id: range2201448
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 2865
end: 3972
orientation: inline

BBa_K149001
type: ComponentDefinition
persistent identity: BBa K149001
display id: BBa_K149001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prp22 promoter
creator: Chris Euler
created: 2008-08-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _243_
m user id: 0
owner id: 3441
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ubiquitous strong promoter.
mutable notes: None
mutable provenance: Simon St. Pierre extracted from S. Cervisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K149001_sequence/1

BBa_K106003
type: ComponentDefinition
persistent identity: BBa K106003
display id: BBa_K106003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sir2, Aar1 BD part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for the Sir2 gene with BD ends for Aar1 cloning. Sir2 is a HDAC that we use for silencing in our project.
mutable notes: Since this is a BD part, there is no ATG but it does include a stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106003_sequence/1

BBa_K801092
type: ComponentDefinition
persistent identity: BBa K801092
display id: BBa_K801092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate - coenzym A ligase (4CL) coding region from ''Arabidopsis thaliana'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 4CL
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801092_sequence/1

4CL
type: SequenceAnnotation
persistent identity: annotation2201482
display id: annotation2201482
version: 1
top level: BBa_K801092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1
type: Range
persistent identity: range2201482
display id: range2201482
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 1689
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2196267
display id: annotation2196267
version: 1
top level: BBa_K801092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1
type: Range
persistent identity: range2196267
display id: range2196267
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1
end: 6
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202114
display id: annotation2202114
version: 1
top level: BBa_K801092
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1
type: Range
persistent identity: range2202114
display id: range2202114
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1690
end: 1692
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201481
display id: annotation2201481
version: 1
top level: BBa_K801092
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1
type: Range
persistent identity: range2201481
display id: range2201481
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 9
orientation: inline

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: primer sequences are coming soon
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

Sin3
type: ComponentDefinition
persistent identity: BBa K165003
display id: BBa_K165003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sin3 Repression Domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a transcriptional repression domain. It can be fused to many DNA-binding domains to create synthetic transcriptional repressors. We have used it upstream of DNA binding domains.
mutable notes: -
mutable provenance: From the yeast genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165003_sequence/1

PolyHb
type: ComponentDefinition
persistent identity: BBa K792007
display id: BBa_K792007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine rich peptide (PolyHb)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble Histidine rich peptide. This part has a '''HindIII''' restriction site as extra.
mutable notes: PolyHb was desing taking into acount the following consideration: # avoided repeating the same residue in tandem to minimize local tRNA depleation # avoided Trp in tandem because of therir low solubility # we included Gly to avoid the formation of stable structures # included acidic and basic amino acids to increase solubility Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Designed by BsAs Team 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: cds
sequence annotation: stop
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792007_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2204263
display id: annotation2204263
version: 1
top level: PolyHb
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2204263/range2204263/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2204263/range2204263/1
type: Range
persistent identity: range2204263
display id: range2204263
version: 1
direction: direction/forward
top level: PolyHb
start: 7
end: 114
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2196617
display id: annotation2196617
version: 1
top level: PolyHb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2196617/range2196617/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2196617/range2196617/1
type: Range
persistent identity: range2196617
display id: range2196617
version: 1
direction: direction/forward
top level: PolyHb
start: 115
end: 120
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196465
display id: annotation2196465
version: 1
top level: PolyHb
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2196465/range2196465/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2196465/range2196465/1
type: Range
persistent identity: range2196465
display id: range2196465
version: 1
direction: direction/forward
top level: PolyHb
start: 1
end: 6
orientation: inline

BBa_K1680022
type: ComponentDefinition
persistent identity: BBa K1680022
display id: BBa_K1680022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:52:38Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 9 AA linker as spacer (BBa_K1680001). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence annotation: Linker
sequence annotation: Cre recombinase
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1680022_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477386
display id: annotation2477386
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1
type: Range
persistent identity: range2477386
display id: range2477386
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 31
end: 699
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477389
display id: annotation2477389
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1
type: Range
persistent identity: range2477389
display id: range2477389
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1756
end: 1783
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477388
display id: annotation2477388
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1
type: Range
persistent identity: range2477388
display id: range2477388
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 727
end: 1755
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477387
display id: annotation2477387
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1
type: Range
persistent identity: range2477387
display id: range2477387
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 700
end: 726
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477385
display id: annotation2477385
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1
type: Range
persistent identity: range2477385
display id: range2477385
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477391
display id: annotation2477391
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1
type: Range
persistent identity: range2477391
display id: range2477391
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1783
end: 2451
orientation: inline

BBa_K1462050
type: ComponentDefinition
persistent identity: BBa K1462050
display id: BBa_K1462050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ccr
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462050_sequence/1

BBa_K801039
type: ComponentDefinition
persistent identity: BBa K801039
display id: BBa_K801039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CDS for a synthetic hybrid fusion protein. Consists of serveral domains. RFC10 pre and suffix
mutable notes: -
mutable provenance: CDS for a synthetic hybrid fusion protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GAL4 activation domain (GAL4AD)
sequence annotation: HA epitope tag
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: EcoRI/MfeI fusion scar
sequence annotation: T7 promoter (for sequencing)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K801039_sequence/1

GAL4 activation domain (GAL4AD)
type: SequenceAnnotation
persistent identity: annotation2199997
display id: annotation2199997
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1
type: Range
persistent identity: range2199997
display id: range2199997
version: 1
direction: direction/forward
top level: BBa_K801039
start: 70
end: 408
orientation: inline

HA epitope tag
type: SequenceAnnotation
persistent identity: annotation2200012
display id: annotation2200012
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1
type: Range
persistent identity: range2200012
display id: range2200012
version: 1
direction: direction/forward
top level: BBa_K801039
start: 457
end: 477
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199929
display id: annotation2199929
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1
type: Range
persistent identity: range2199929
display id: range2199929
version: 1
direction: direction/forward
top level: BBa_K801039
start: 31
end: 51
orientation: inline

EcoRI/MfeI fusion scar
type: SequenceAnnotation
persistent identity: annotation2200000
display id: annotation2200000
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1
type: Range
persistent identity: range2200000
display id: range2200000
version: 1
direction: direction/forward
top level: BBa_K801039
start: 499
end: 504
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2200010
display id: annotation2200010
version: 1
top level: BBa_K801039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1
type: Range
persistent identity: range2200010
display id: range2200010
version: 1
direction: direction/forward
top level: BBa_K801039
start: 414
end: 436
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2199998
display id: annotation2199998
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1
type: Range
persistent identity: range2199998
display id: range2199998
version: 1
direction: direction/forward
top level: BBa_K801039
start: 503
end: 801
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2200008
display id: annotation2200008
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1
type: Range
persistent identity: range2200008
display id: range2200008
version: 1
direction: direction/forward
top level: BBa_K801039
start: 409
end: 498
orientation: inline

BBa_K1680018
type: ComponentDefinition
persistent identity: BBa K1680018
display id: BBa_K1680018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:36Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly and RFC25.
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680001
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680018_sequence/1

K1680001
type: SequenceAnnotation
persistent identity: annotation2477357
display id: annotation2477357
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1
type: Range
persistent identity: range2477357
display id: range2477357
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1
end: 21
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477358
display id: annotation2477358
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1
type: Range
persistent identity: range2477358
display id: range2477358
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1051
end: 1077
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477354
display id: annotation2477354
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1
type: Range
persistent identity: range2477354
display id: range2477354
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1078
end: 1746
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477355
display id: annotation2477355
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1
type: Range
persistent identity: range2477355
display id: range2477355
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 22
end: 1050
orientation: inline

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165031
component: BBa_K165011
sequence annotation: BBa_K165011
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165031

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 1
end: 46
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 55
end: 457
orientation: inline

PolyWb
type: ComponentDefinition
persistent identity: BBa K792008
display id: BBa_K792008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tryptophan rich peptide (PolyWb)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble '''Histidine''' rich peptide (designed in silico) This part has a '''HindIII''' restriction site as extra.
mutable notes: PolyWb was desing taking into acount the following consideration: # avoided repeating the same residue in tandem to minimize local tRNA depleation # avoided Trp in tandem because of therir low solubility # we included Gly to avoid the formation of stable structures # included acidic and basic amino acids to increase solubility Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Designed by BsAs Team 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HindIII restriction site
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K792008_sequence/1

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196471
display id: annotation2196471
version: 1
top level: PolyWb
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2196471/range2196471/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2196471/range2196471/1
type: Range
persistent identity: range2196471
display id: range2196471
version: 1
direction: direction/forward
top level: PolyWb
start: 1
end: 6
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2204264
display id: annotation2204264
version: 1
top level: PolyWb
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2204264/range2204264/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2204264/range2204264/1
type: Range
persistent identity: range2204264
display id: range2204264
version: 1
direction: direction/forward
top level: PolyWb
start: 7
end: 114
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2196618
display id: annotation2196618
version: 1
top level: PolyWb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2196618/range2196618/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2196618/range2196618/1
type: Range
persistent identity: range2196618
display id: range2196618
version: 1
direction: direction/forward
top level: PolyWb
start: 115
end: 120
orientation: inline

BBa_K801097
type: ComponentDefinition
persistent identity: BBa K801097
display id: BBa_K801097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Chalcone O-methyltransferase (OMT1) coding region from ''Humulus lupulus'' preceeded by yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nucleotide/167613934/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: OMT
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_K801097_sequence/1

OMT
type: SequenceAnnotation
persistent identity: annotation2202172
display id: annotation2202172
version: 1
top level: BBa_K801097
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202172/range2202172/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202172/range2202172/1
type: Range
persistent identity: range2202172
display id: range2202172
version: 1
direction: direction/forward
top level: BBa_K801097
start: 7
end: 1062
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202170
display id: annotation2202170
version: 1
top level: BBa_K801097
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202170/range2202170/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202170/range2202170/1
type: Range
persistent identity: range2202170
display id: range2202170
version: 1
direction: direction/forward
top level: BBa_K801097
start: 7
end: 9
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2202171
display id: annotation2202171
version: 1
top level: BBa_K801097
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202171/range2202171/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202171/range2202171/1
type: Range
persistent identity: range2202171
display id: range2202171
version: 1
direction: direction/forward
top level: BBa_K801097
start: 1
end: 6
orientation: inline

BBa_K165001
type: ComponentDefinition
persistent identity: BBa K165001
display id: BBa_K165001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1+ w/XhoI sites
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
mutable notes: none
mutable provenance: From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165001_sequence/1

BBa_K165049
type: ComponentDefinition
persistent identity: BBa K165049
display id: BBa_K165049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full LexA Repressor (tagges with mCherryx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+LexA DBD+Sin3p RD+mCherryx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165004
component: BBa_K165018
component: BBa_K165003
component: BBa_K165002
component: BBa_K165009
sequence annotation: BBa_K165004
sequence annotation: BBa_K165009
sequence annotation: BBa_K165003
sequence annotation: BBa_K165018
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165049_sequence/1

BBa_K165004
type: Component
persistent identity: component1996676
display id: component1996676
version: 1
top level: BBa_K165049
access: public
definition: mCherry

BBa_K165018
type: Component
persistent identity: component1996677
display id: component1996677
version: 1
top level: BBa_K165049
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165003
type: Component
persistent identity: component1996674
display id: component1996674
version: 1
top level: BBa_K165049
access: public
definition: Sin3

BBa_K165002
type: Component
persistent identity: component1996672
display id: component1996672
version: 1
top level: BBa_K165049
access: public
definition: BBa_K165002

BBa_K165009
type: Component
persistent identity: component1996673
display id: component1996673
version: 1
top level: BBa_K165049
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165004
type: Component
persistent identity: component1996675
display id: component1996675
version: 1
top level: BBa_K165049
access: public
definition: mCherry

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996675
display id: annotation1996675
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996675/range1996675/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165049/annotation1996675/range1996675/1
type: Range
persistent identity: range1996675
display id: range1996675
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 3112
end: 3846
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1996673
display id: annotation1996673
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996673/range1996673/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165049/annotation1996673/range1996673/1
type: Range
persistent identity: range1996673
display id: range1996673
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 27
end: 284
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996674
display id: annotation1996674
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996674/range1996674/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165049/annotation1996674/range1996674/1
type: Range
persistent identity: range1996674
display id: range1996674
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 293
end: 3103
orientation: inline

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1996676
display id: annotation1996676
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996676/range1996676/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165049/annotation1996676/range1996676/1
type: Range
persistent identity: range1996676
display id: range1996676
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 3855
end: 4589
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996677
display id: annotation1996677
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996677/range1996677/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165049/annotation1996677/range1996677/1
type: Range
persistent identity: range1996677
display id: range1996677
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 4598
end: 4850
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996672
display id: annotation1996672
version: 1
top level: BBa_K165049
location: https://synbiohub.org/public/igem/BBa_K165049/annotation1996672/range1996672/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165049/annotation1996672/range1996672/1
type: Range
persistent identity: range1996672
display id: range1996672
version: 1
direction: direction/reverse
top level: BBa_K165049
start: 1
end: 18
orientation: inline

HBsAg
type: ComponentDefinition
persistent identity: BBa K1908002
display id: BBa_K1908002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Chromoprotein + 2a + HBsAg + Yeast Secretion Tag + ADH1 Terminator
creator: Brian Yang, Jonathan Krog, Sunil Deochand, Jonathan Kelly
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T12:14:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: S. Cerevisiae Kozak Sequence
sequence annotation: HBsAg
sequence annotation: Yeast Secretion Tag
sequence annotation: amilCP - Blue Chromoprotein
sequence annotation: 2A Peptide
sequence annotation: ADH1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1908002_sequence/1

S. Cerevisiae Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2521820
display id: annotation2521820
version: 1
top level: HBsAg
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521820/range2521820/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521820/range2521820/1
type: Range
persistent identity: range2521820
display id: range2521820
version: 1
direction: direction/forward
top level: HBsAg
start: 1
end: 10
orientation: inline

HBsAg
type: SequenceAnnotation
persistent identity: annotation2521924
display id: annotation2521924
version: 1
top level: HBsAg
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521924/range2521924/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521924/range2521924/1
type: Range
persistent identity: range2521924
display id: range2521924
version: 1
direction: direction/forward
top level: HBsAg
start: 740
end: 1417
orientation: inline

Yeast Secretion Tag
type: SequenceAnnotation
persistent identity: annotation2521953
display id: annotation2521953
version: 1
top level: HBsAg
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521953/range2521953/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521953/range2521953/1
type: Range
persistent identity: range2521953
display id: range2521953
version: 1
direction: direction/forward
top level: HBsAg
start: 1418
end: 1537
orientation: inline

amilCP - Blue Chromoprotein
type: SequenceAnnotation
persistent identity: annotation2521893
display id: annotation2521893
version: 1
top level: HBsAg
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521893/range2521893/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521893/range2521893/1
type: Range
persistent identity: range2521893
display id: range2521893
version: 1
direction: direction/forward
top level: HBsAg
start: 11
end: 682
orientation: inline

2A Peptide
type: SequenceAnnotation
persistent identity: annotation2521934
display id: annotation2521934
version: 1
top level: HBsAg
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2521934/range2521934/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2521934/range2521934/1
type: Range
persistent identity: range2521934
display id: range2521934
version: 1
direction: direction/forward
top level: HBsAg
start: 683
end: 739
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2522045
display id: annotation2522045
version: 1
top level: HBsAg
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908002/annotation2522045/range2522045/1

https://synbiohub.org/public/igem/BBa_K1908002/annotation2522045/range2522045/1
type: Range
persistent identity: range2522045
display id: range2522045
version: 1
direction: direction/forward
top level: HBsAg
start: 1538
end: 1666
orientation: inline

BBa_K1680016
type: ComponentDefinition
persistent identity: BBa K1680016
display id: BBa_K1680016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS316 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pUC origin
sequence annotation: T7 promotor
sequence annotation: ampR
sequence annotation: URA3
sequence annotation: T3 promotor
sequence annotation: f1 origin
sequence annotation: ampR promotor
sequence annotation: URA3 promotor
sequence annotation: lacZalpha
sequence annotation: lac promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: RFC10 MCS
sequence: https://synbiohub.org/public/igem/BBa_K1680016_sequence/1

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466222
display id: annotation2466222
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1
type: Range
persistent identity: range2466222
display id: range2466222
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2515
end: 3103
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466214
display id: annotation2466214
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1
type: Range
persistent identity: range2466214
display id: range2466214
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 1966
end: 1985
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466235
display id: annotation2466235
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1
type: Range
persistent identity: range2466235
display id: range2466235
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 3274
end: 4134
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2466209
display id: annotation2466209
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1
type: Range
persistent identity: range2466209
display id: range2466209
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 411
end: 1214
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466219
display id: annotation2466219
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1
type: Range
persistent identity: range2466219
display id: range2466219
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2073
end: 2092
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466211
display id: annotation2466211
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1
type: Range
persistent identity: range2466211
display id: range2466211
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1343
end: 1798
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466236
display id: annotation2466236
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1
type: Range
persistent identity: range2466236
display id: range2466236
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 4135
end: 4239
orientation: inline

URA3 promotor
type: SequenceAnnotation
persistent identity: annotation2466206
display id: annotation2466206
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1
type: Range
persistent identity: range2466206
display id: range2466206
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 195
end: 410
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466213
display id: annotation2466213
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1
type: Range
persistent identity: range2466213
display id: range2466213
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1581
end: 1997
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466221
display id: annotation2466221
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1
type: Range
persistent identity: range2466221
display id: range2466221
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2161
end: 2191
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466237
display id: annotation2466237
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1
type: Range
persistent identity: range2466237
display id: range2466237
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 4276
end: 4779
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466217
display id: annotation2466217
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1
type: Range
persistent identity: range2466217
display id: range2466217
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2055
end: 2117
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466215
display id: annotation2466215
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1
type: Range
persistent identity: range2466215
display id: range2466215
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2003
end: 2045
orientation: inline

mOrange
type: ComponentDefinition
persistent identity: BBa K105011
display id: BBa_K105011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mOrange, Yeast optimized for fusion proteins
creator: Marius M??ller, Paul Bourgine
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is red-orange fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of a BioBrick part for fusion proteins (see design details). <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of BBa_E2050 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105011_sequence/1

BBa_K416004
type: ComponentDefinition
persistent identity: BBa K416004
display id: BBa_K416004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2:(Gly3Ser)4 Linker Composite Part
creator: Russell Durrett
created: 2010-07-18T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite part consists of the Aga2 yeast surface display CDS linked to a flexible (Glycine3Ser)4 linker. It can be used by linking it upstream of the protein you wish to be displayed on the surface of the yeast cell. Note: Aga1 must be constitutively expressed by the yeast strain for Aga2 to anchor to the cellular surface.
mutable notes: This composite part was put together following RFC23, thus the scar between parts consists of the sequence ACTAGA
mutable provenance: This part comes from the Aga2 [K416000] and Linker [K416001] parts we constructed this year.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K416000
component: BBa_K416001
sequence annotation: BBa_K416001
sequence annotation: BBa_K416000
sequence: https://synbiohub.org/public/igem/BBa_K416004_sequence/1

BBa_K416000
type: Component
persistent identity: component2074507
display id: component2074507
version: 1
top level: BBa_K416004
access: public
definition: BBa_K416000

BBa_K416001
type: Component
persistent identity: component2074508
display id: component2074508
version: 1
top level: BBa_K416004
access: public
definition: https://synbiohub.org/public/igem/BBa_K416001/1

BBa_K416001
type: SequenceAnnotation
persistent identity: annotation2074508
display id: annotation2074508
version: 1
top level: BBa_K416004
location: https://synbiohub.org/public/igem/BBa_K416004/annotation2074508/range2074508/1
component: BBa_K416001

https://synbiohub.org/public/igem/BBa_K416004/annotation2074508/range2074508/1
type: Range
persistent identity: range2074508
display id: range2074508
version: 1
direction: direction/reverse
top level: BBa_K416004
start: 270
end: 314
orientation: inline

BBa_K416000
type: SequenceAnnotation
persistent identity: annotation2074507
display id: annotation2074507
version: 1
top level: BBa_K416004
location: https://synbiohub.org/public/igem/BBa_K416004/annotation2074507/range2074507/1
component: BBa_K416000

https://synbiohub.org/public/igem/BBa_K416004/annotation2074507/range2074507/1
type: Range
persistent identity: range2074507
display id: range2074507
version: 1
direction: direction/reverse
top level: BBa_K416004
start: 1
end: 261
orientation: inline

BBa_K976008
type: ComponentDefinition
persistent identity: BBa K976008
display id: BBa_K976008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast ADH1 Promoter + Firefly Luciferase + Yeast ADH1 Promoter + Renilla Luciferase
creator: Amna Sohail & Molly Petrous & Tyler Knox & Mackenzie Reetz
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Yeast Promoter + Luciferase + Yeast Promoter + Renilla
mutable notes: None.
mutable provenance: NCBI GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J63005
component: BBa_J52008
component: BBa_I712019
sequence annotation: BBa_I712019
sequence annotation: BBa_J63005
sequence annotation: BBa_J52008
sequence: https://synbiohub.org/public/igem/BBa_K976008_sequence/1

BBa_J63005
type: Component
persistent identity: component2329829
display id: component2329829
version: 1
top level: BBa_K976008
access: public
definition: P0310

BBa_J52008
type: Component
persistent identity: component2329836
display id: component2329836
version: 1
top level: BBa_K976008
access: public
definition: https://synbiohub.org/public/igem/BBa_J52008/1

BBa_J63005
type: Component
persistent identity: component2329833
display id: component2329833
version: 1
top level: BBa_K976008
access: public
definition: P0310

BBa_I712019
type: Component
persistent identity: component2329830
display id: component2329830
version: 1
top level: BBa_K976008
access: public
definition: https://synbiohub.org/public/igem/BBa_I712019/1

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2329830
display id: annotation2329830
version: 1
top level: BBa_K976008
location: https://synbiohub.org/public/igem/BBa_K976008/annotation2329830/range2329830/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K976008/annotation2329830/range2329830/1
type: Range
persistent identity: range2329830
display id: range2329830
version: 1
direction: direction/reverse
top level: BBa_K976008
start: 1452
end: 3104
orientation: inline

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2329833
display id: annotation2329833
version: 1
top level: BBa_K976008
location: https://synbiohub.org/public/igem/BBa_K976008/annotation2329833/range2329833/1
component: BBa_J63005

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type: Range
persistent identity: range2329833
display id: range2329833
version: 1
direction: direction/reverse
top level: BBa_K976008
start: 3113
end: 4557
orientation: inline

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2329829
display id: annotation2329829
version: 1
top level: BBa_K976008
location: https://synbiohub.org/public/igem/BBa_K976008/annotation2329829/range2329829/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K976008/annotation2329829/range2329829/1
type: Range
persistent identity: range2329829
display id: range2329829
version: 1
direction: direction/reverse
top level: BBa_K976008
start: 1
end: 1445
orientation: inline

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2329836
display id: annotation2329836
version: 1
top level: BBa_K976008
location: https://synbiohub.org/public/igem/BBa_K976008/annotation2329836/range2329836/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K976008/annotation2329836/range2329836/1
type: Range
persistent identity: range2329836
display id: range2329836
version: 1
direction: direction/reverse
top level: BBa_K976008
start: 4564
end: 5499
orientation: inline

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast GPD (TDH3) Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

BBa_K105031
type: ComponentDefinition
persistent identity: BBa K105031
display id: BBa_K105031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc16 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105031_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989843
display id: annotation1989843
version: 1
top level: BBa_K105031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1
type: Range
persistent identity: range1989843
display id: range1989843
version: 1
direction: direction/forward
top level: BBa_K105031
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989845
display id: annotation1989845
version: 1
top level: BBa_K105031
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1
type: Range
persistent identity: range1989845
display id: range1989845
version: 1
direction: direction/forward
top level: BBa_K105031
start: 22
end: 22
orientation: inline

BBa_K165096
type: ComponentDefinition
persistent identity: BBa K165096
display id: BBa_K165096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + MET25+ Gli1 repressor (CFPx2 tagged) on pRS304*
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165007
component: BBa_K165003
component: BBa_K165011
component: BBa_K165018
component: BBa_K165014
component: BBa_K165000
component: BBa_K165002
sequence annotation: BBa_K165018
sequence annotation: BBa_K165007
sequence annotation: BBa_K165011
sequence annotation: BBa_K165003
sequence annotation: BBa_K165000
sequence annotation: BBa_K165014
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165096_sequence/1

BBa_K165007
type: Component
persistent identity: component1997364
display id: component1997364
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K165003
type: Component
persistent identity: component1997363
display id: component1997363
version: 1
top level: BBa_K165096
access: public
definition: Sin3

BBa_K165011
type: Component
persistent identity: component1997360
display id: component1997360
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165018
type: Component
persistent identity: component1997366
display id: component1997366
version: 1
top level: BBa_K165096
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165014
type: Component
persistent identity: component1997365
display id: component1997365
version: 1
top level: BBa_K165096
access: public
definition: cfpx2

BBa_K165000
type: Component
persistent identity: component1997361
display id: component1997361
version: 1
top level: BBa_K165096
access: public
definition: BBa_K165000

BBa_K165002
type: Component
persistent identity: component1997362
display id: component1997362
version: 1
top level: BBa_K165096
access: public
definition: BBa_K165002

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1997366
display id: annotation1997366
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997366/range1997366/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165096/annotation1997366/range1997366/1
type: Range
persistent identity: range1997366
display id: range1997366
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 5330
end: 5582
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation1997364
display id: annotation1997364
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997364/range1997364/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K165096/annotation1997364/range1997364/1
type: Range
persistent identity: range1997364
display id: range1997364
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 3295
end: 3819
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997360
display id: annotation1997360
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997360/range1997360/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165096/annotation1997360/range1997360/1
type: Range
persistent identity: range1997360
display id: range1997360
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 1
end: 46
orientation: inline

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1997363
display id: annotation1997363
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997363/range1997363/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165096/annotation1997363/range1997363/1
type: Range
persistent identity: range1997363
display id: range1997363
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 476
end: 3286
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1997361
display id: annotation1997361
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997361/range1997361/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165096/annotation1997361/range1997361/1
type: Range
persistent identity: range1997361
display id: range1997361
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 55
end: 441
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1997365
display id: annotation1997365
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997365/range1997365/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165096/annotation1997365/range1997365/1
type: Range
persistent identity: range1997365
display id: range1997365
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 3828
end: 5321
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997362
display id: annotation1997362
version: 1
top level: BBa_K165096
location: https://synbiohub.org/public/igem/BBa_K165096/annotation1997362/range1997362/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165096/annotation1997362/range1997362/1
type: Range
persistent identity: range1997362
display id: range1997362
version: 1
direction: direction/reverse
top level: BBa_K165096
start: 450
end: 467
orientation: inline

BBa_K106699
type: ComponentDefinition
persistent identity: BBa K106699
display id: BBa_K106699
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal1 Promoter
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Galactose-inducible promoter (strong)!
mutable notes: None.
mutable provenance: Yeast genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K106699_sequence/1

BBa_K106002
type: ComponentDefinition
persistent identity: BBa K106002
display id: BBa_K106002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sir2, Aar1 AB part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for the Sir2 gene with AB ends for Aar1 cloning. Sir2 is a HDAC that we use for silencing in our project.
mutable notes: Since this is an AB part, there is a ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106002_sequence/1

BBa_K1486036
type: ComponentDefinition
persistent identity: BBa K1486036
display id: BBa_K1486036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucC + ADH1 terminator + CaUra3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486031
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486020
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486031
sequence: https://synbiohub.org/public/igem/BBa_K1486036_sequence/1

BBa_K1486031
type: Component
persistent identity: component2418343
display id: component2418343
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486031

BBa_K1486025
type: Component
persistent identity: component2418339
display id: component2418339
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2418337
display id: component2418337
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486020
type: Component
persistent identity: component2418338
display id: component2418338
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418337
display id: annotation2418337
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
type: Range
persistent identity: range2418337
display id: range2418337
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418339
display id: annotation2418339
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
type: Range
persistent identity: range2418339
display id: range2418339
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 646
end: 833
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418338
display id: annotation2418338
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
type: Range
persistent identity: range2418338
display id: range2418338
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 40
end: 645
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418343
display id: annotation2418343
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
type: Range
persistent identity: range2418343
display id: range2418343
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 834
end: 2193
orientation: inline

BBa_K1486027
type: ComponentDefinition
persistent identity: BBa K1486027
display id: BBa_K1486027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486037
component: BBa_K1486020
component: BBa_K1486024
component: BBa_K1486019
component: BBa_K801012
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486019
sequence annotation: BBa_K801012
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486027_sequence/1

BBa_K1486037
type: Component
persistent identity: component2417706
display id: component2417706
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486020
type: Component
persistent identity: component2417708
display id: component2417708
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486024
type: Component
persistent identity: component2417714
display id: component2417714
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486024

BBa_K1486019
type: Component
persistent identity: component2417707
display id: component2417707
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K801012
type: Component
persistent identity: component2417710
display id: component2417710
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K801012

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2417708
display id: annotation2417708
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
type: Range
persistent identity: range2417708
display id: range2417708
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 370
end: 975
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417707
display id: annotation2417707
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
type: Range
persistent identity: range2417707
display id: range2417707
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 40
end: 369
orientation: inline

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2417710
display id: annotation2417710
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
type: Range
persistent identity: range2417710
display id: range2417710
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 976
end: 1324
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417706
display id: annotation2417706
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
type: Range
persistent identity: range2417706
display id: range2417706
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1
end: 39
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417714
display id: annotation2417714
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
type: Range
persistent identity: range2417714
display id: range2417714
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1325
end: 2681
orientation: inline

BBa_K1680007
type: ComponentDefinition
persistent identity: BBa K1680007
display id: BBa_K1680007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre recombinase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:34:10Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the DNA recombinase Cre, codon optimized for yeast. The Cre recombinase recognizes two loxP sites (Part: BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680007_sequence/1

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477356
display id: annotation2477356
version: 1
top level: BBa_K1680007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680007/annotation2477356/range2477356/1

https://synbiohub.org/public/igem/BBa_K1680007/annotation2477356/range2477356/1
type: Range
persistent identity: range2477356
display id: range2477356
version: 1
direction: direction/forward
top level: BBa_K1680007
start: 1
end: 1029
orientation: inline

BBa_K1728022
type: ComponentDefinition
persistent identity: BBa K1728022
display id: BBa_K1728022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LUCY tag
creator: Wan-Yun Chiu
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T01:32:36Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2149_
m user id: 18379
owner id: 18379
owning group id: 9
status: status/Planning
bookmark: false
mutable description: LUCY tag
mutable notes: No
mutable provenance: Order the DNA part from synthesis company
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1728022_sequence/1

mCherry
type: ComponentDefinition
persistent identity: BBa J63000
display id: BBa_J63000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast codon-optimized mCherry
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: yeast codon-optimized mCherry
mutable notes: as published
mutable provenance: Thorn et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence: https://synbiohub.org/public/igem/BBa_J63000_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1902849
display id: annotation1902849
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63000/annotation1902849/range1902849/1

https://synbiohub.org/public/igem/BBa_J63000/annotation1902849/range1902849/1
type: Range
persistent identity: range1902849
display id: range1902849
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 735
orientation: inline

BBa_K165036
type: ComponentDefinition
persistent identity: BBa K165036
display id: BBa_K165036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165031
sequence annotation: BBa_K165012
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165036_sequence/1

BBa_K165012
type: Component
persistent identity: component1995510
display id: component1995510
version: 1
top level: BBa_K165036
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165031
type: Component
persistent identity: component1995511
display id: component1995511
version: 1
top level: BBa_K165036
access: public
definition: BBa_K165031

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995510
display id: annotation1995510
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
type: Range
persistent identity: range1995510
display id: range1995510
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 1
end: 127
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995511
display id: annotation1995511
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
type: Range
persistent identity: range1995511
display id: range1995511
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 136
end: 538
orientation: inline

BBa_K801003
type: ComponentDefinition
persistent identity: BBa K801003
display id: BBa_K801003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM103 yeast shuttle vector with pADH1 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: URA3
sequence annotation: ADH1-Promoter
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence annotation: T7-Promoter
sequence annotation: 2 micron (??) origin
sequence annotation: CYC1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801003_sequence/1

URA3
type: SequenceAnnotation
persistent identity: annotation2201841
display id: annotation2201841
version: 1
top level: BBa_K801003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201841/range2201841/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201841/range2201841/1
type: Range
persistent identity: range2201841
display id: range2201841
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 2936
end: 4043
orientation: inline

ADH1-Promoter
type: SequenceAnnotation
persistent identity: annotation2201844
display id: annotation2201844
version: 1
top level: BBa_K801003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201844/range2201844/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201844/range2201844/1
type: Range
persistent identity: range2201844
display id: range2201844
version: 1
direction: direction/forward
top level: BBa_K801003
start: 58
end: 775
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201911
display id: annotation2201911
version: 1
top level: BBa_K801003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201911/range2201911/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201911/range2201911/1
type: Range
persistent identity: range2201911
display id: range2201911
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 1240
end: 1913
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201842
display id: annotation2201842
version: 1
top level: BBa_K801003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201842/range2201842/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201842/range2201842/1
type: Range
persistent identity: range2201842
display id: range2201842
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 2058
end: 2917
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201845
display id: annotation2201845
version: 1
top level: BBa_K801003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201845/range2201845/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201845/range2201845/1
type: Range
persistent identity: range2201845
display id: range2201845
version: 1
direction: direction/forward
top level: BBa_K801003
start: 4
end: 23
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201910
display id: annotation2201910
version: 1
top level: BBa_K801003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201910/range2201910/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201910/range2201910/1
type: Range
persistent identity: range2201910
display id: range2201910
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 4047
end: 5518
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2201840
display id: annotation2201840
version: 1
top level: BBa_K801003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201840/range2201840/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201840/range2201840/1
type: Range
persistent identity: range2201840
display id: range2201840
version: 1
direction: direction/forward
top level: BBa_K801003
start: 810
end: 1057
orientation: inline

BBa_K1871001
type: ComponentDefinition
persistent identity: BBa K1871001
display id: BBa_K1871001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha amylase with native secretion
creator: Broad Run High School iGEM Team
created: 2015-09-12T11:00:00Z
modified: 2015-12-17T01:16:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2303_
m user id: 4206
owner id: 28667
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence (Part BBa_K165002) the ADH1 terminator (Part BBa_K392003) . Expression is controlled by the cyc yeast promoter (Part BBa_K105027).
mutable notes: TBD
mutable provenance: The amylase gene was obtained from the published sequence for Bacillus amyloliquefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Kozak Sequence
sequence annotation: Native Alpha Amylase Secretion Sequence
sequence annotation: ADH1 Terminator
sequence annotation: Alpha Amylase Coding Sequence
sequence annotation: Minimal cyc Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1871001_sequence/1

Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2462560
display id: annotation2462560
version: 1
top level: BBa_K1871001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1871001/annotation2462560/range2462560/1

https://synbiohub.org/public/igem/BBa_K1871001/annotation2462560/range2462560/1
type: Range
persistent identity: range2462560
display id: range2462560
version: 1
direction: direction/forward
top level: BBa_K1871001
start: 105
end: 125
orientation: inline

Native Alpha Amylase Secretion Sequence
type: SequenceAnnotation
persistent identity: annotation2462565
display id: annotation2462565
version: 1
top level: BBa_K1871001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1871001/annotation2462565/range2462565/1

https://synbiohub.org/public/igem/BBa_K1871001/annotation2462565/range2462565/1
type: Range
persistent identity: range2462565
display id: range2462565
version: 1
direction: direction/forward
top level: BBa_K1871001
start: 126
end: 215
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2462567
display id: annotation2462567
version: 1
top level: BBa_K1871001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1871001/annotation2462567/range2462567/1

https://synbiohub.org/public/igem/BBa_K1871001/annotation2462567/range2462567/1
type: Range
persistent identity: range2462567
display id: range2462567
version: 1
direction: direction/forward
top level: BBa_K1871001
start: 1668
end: 1855
orientation: inline

Alpha Amylase Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2462566
display id: annotation2462566
version: 1
top level: BBa_K1871001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1871001/annotation2462566/range2462566/1

https://synbiohub.org/public/igem/BBa_K1871001/annotation2462566/range2462566/1
type: Range
persistent identity: range2462566
display id: range2462566
version: 1
direction: direction/forward
top level: BBa_K1871001
start: 216
end: 1667
orientation: inline

Minimal cyc Promoter
type: SequenceAnnotation
persistent identity: annotation2462558
display id: annotation2462558
version: 1
top level: BBa_K1871001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1871001/annotation2462558/range2462558/1

https://synbiohub.org/public/igem/BBa_K1871001/annotation2462558/range2462558/1
type: Range
persistent identity: range2462558
display id: range2462558
version: 1
direction: direction/forward
top level: BBa_K1871001
start: 1
end: 104
orientation: inline

BBa_K976007
type: ComponentDefinition
persistent identity: BBa K976007
display id: BBa_K976007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast ADH1 promoter + Renilla Luciferase
creator: Amna Sohail & Molly Petrous & Tyler Knox & Mackenzie Reetz
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Yeast Promoter + Renilla Composite
mutable notes: None.
mutable provenance: NCBI GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52008
component: BBa_J63005
sequence annotation: BBa_J52008
sequence annotation: BBa_J63005
sequence: https://synbiohub.org/public/igem/BBa_K976007_sequence/1

BBa_J52008
type: Component
persistent identity: component2329826
display id: component2329826
version: 1
top level: BBa_K976007
access: public
definition: https://synbiohub.org/public/igem/BBa_J52008/1

BBa_J63005
type: Component
persistent identity: component2329823
display id: component2329823
version: 1
top level: BBa_K976007
access: public
definition: P0310

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2329826
display id: annotation2329826
version: 1
top level: BBa_K976007
location: https://synbiohub.org/public/igem/BBa_K976007/annotation2329826/range2329826/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K976007/annotation2329826/range2329826/1
type: Range
persistent identity: range2329826
display id: range2329826
version: 1
direction: direction/reverse
top level: BBa_K976007
start: 1452
end: 2387
orientation: inline

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2329823
display id: annotation2329823
version: 1
top level: BBa_K976007
location: https://synbiohub.org/public/igem/BBa_K976007/annotation2329823/range2329823/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K976007/annotation2329823/range2329823/1
type: Range
persistent identity: range2329823
display id: range2329823
version: 1
direction: direction/reverse
top level: BBa_K976007
start: 1
end: 1445
orientation: inline

BBa_K394000
type: ComponentDefinition
persistent identity: BBa K394000
display id: BBa_K394000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Resistance Gene for G418 in Yeast
creator: Brian Landry
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part confers resistance to Geneticin (G418) in yeast. Selection of yeast cells is typically preformed on plates with 200??g/ml of G418.
mutable notes: None
mutable provenance: The part was synthesized from the PMKV005 plasmid used in McCusker et. al. The coding region contains the KanR open reading from transposon Tn903 from E. coli. 1. McCusker JH, Vorachek-Warren MK. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast (Chichester, England). 2004;21(2):163-71. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14755641.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Stop
sequence annotation: KanR gene
sequence annotation: HindIII cut site
sequence: https://synbiohub.org/public/igem/BBa_K394000_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2104020
display id: annotation2104020
version: 1
top level: BBa_K394000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1
type: Range
persistent identity: range2104020
display id: range2104020
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2104023
display id: annotation2104023
version: 1
top level: BBa_K394000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1
type: Range
persistent identity: range2104023
display id: range2104023
version: 1
direction: direction/forward
top level: BBa_K394000
start: 808
end: 813
orientation: inline

KanR gene
type: SequenceAnnotation
persistent identity: annotation2104021
display id: annotation2104021
version: 1
top level: BBa_K394000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1
type: Range
persistent identity: range2104021
display id: range2104021
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 810
orientation: inline

HindIII cut site
type: SequenceAnnotation
persistent identity: annotation2104022
display id: annotation2104022
version: 1
top level: BBa_K394000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1
type: Range
persistent identity: range2104022
display id: range2104022
version: 1
direction: direction/forward
top level: BBa_K394000
start: 545
end: 549
orientation: inline

BBa_Y00073
type: ComponentDefinition
persistent identity: BBa Y00073
display id: BBa_Y00073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. pombe gene SPCC126.04c, coded for S. cerevisiae expression
creator: Natalie Kuldell
created: 2006-06-21T11:00:00Z
modified: 2015-05-08T01:14:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _43_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SPCC126.04c is the pombe homolog of SGF73, a subunit of the SAGA ATP-independent chromatin remodelling complex in S. cerevisiae. Deletion of this subunit from S. cerevisiae leads to viable cells but poor expression of PAU genes under hypoxic conditions. Other phenotypes may also exist but are currently undescribed. The part BBa_Y00073 is the pombe SPCC126.04c ORF (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=68037958&itemID=1&view=gbwithparts) that has been codon optimized by DNA2.0 for cerevisiae expression. This may or may not be needed for optimal expression in cerevisiae, though the recoding does leave only 77% (794/1028) bp identities.
mutable notes: codon optimization for expression in new species
mutable provenance: S. pombe gene recoded for S. cerevisiae expression
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: prefix
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/BBa_Y00073_sequence/1

prefix
type: SequenceAnnotation
persistent identity: annotation1881734
display id: annotation1881734
version: 1
top level: BBa_Y00073
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_Y00073/annotation1881734/range1881734/1

https://synbiohub.org/public/igem/BBa_Y00073/annotation1881734/range1881734/1
type: Range
persistent identity: range1881734
display id: range1881734
version: 1
direction: direction/forward
top level: BBa_Y00073
start: 1
end: 30
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1881735
display id: annotation1881735
version: 1
top level: BBa_Y00073
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_Y00073/annotation1881735/range1881735/1

https://synbiohub.org/public/igem/BBa_Y00073/annotation1881735/range1881735/1
type: Range
persistent identity: range1881735
display id: range1881735
version: 1
direction: direction/forward
top level: BBa_Y00073
start: 1066
end: 1094
orientation: inline

BBa_K165088
type: ComponentDefinition
persistent identity: BBa K165088
display id: BBa_K165088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + TEF2 + Untagged LexA activator on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165037
component: BBa_K165011
component: BBa_K165020
sequence annotation: BBa_K165037
sequence annotation: BBa_K165020
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165088_sequence/1

BBa_K165037
type: Component
persistent identity: component1997204
display id: component1997204
version: 1
top level: BBa_K165088
access: public
definition: pTEF

BBa_K165011
type: Component
persistent identity: component1997203
display id: component1997203
version: 1
top level: BBa_K165088
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165020
type: Component
persistent identity: component1997205
display id: component1997205
version: 1
top level: BBa_K165088
access: public
definition: lexA

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997204
display id: annotation1997204
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997204/range1997204/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165088/annotation1997204/range1997204/1
type: Range
persistent identity: range1997204
display id: range1997204
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 55
end: 457
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997205
display id: annotation1997205
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997205/range1997205/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165088/annotation1997205/range1997205/1
type: Range
persistent identity: range1997205
display id: range1997205
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 466
end: 1137
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1997203
display id: annotation1997203
version: 1
top level: BBa_K165088
location: https://synbiohub.org/public/igem/BBa_K165088/annotation1997203/range1997203/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165088/annotation1997203/range1997203/1
type: Range
persistent identity: range1997203
display id: range1997203
version: 1
direction: direction/reverse
top level: BBa_K165088
start: 1
end: 46
orientation: inline

BBa_K165089
type: ComponentDefinition
persistent identity: BBa K165089
display id: BBa_K165089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + TEF2 + Untagged LexA activator on pRS306
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:57Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K165037
component: BBa_K165012
component: BBa_K165020
sequence annotation: BBa_K165037
sequence annotation: BBa_K165012
sequence annotation: BBa_K165020
sequence: https://synbiohub.org/public/igem/BBa_K165089_sequence/1

BBa_K165037
type: Component
persistent identity: component1997207
display id: component1997207
version: 1
top level: BBa_K165089
access: public
definition: pTEF

BBa_K165012
type: Component
persistent identity: component1997206
display id: component1997206
version: 1
top level: BBa_K165089
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165020
type: Component
persistent identity: component1997208
display id: component1997208
version: 1
top level: BBa_K165089
access: public
definition: lexA

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1997207
display id: annotation1997207
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997207/range1997207/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165089/annotation1997207/range1997207/1
type: Range
persistent identity: range1997207
display id: range1997207
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1997206
display id: annotation1997206
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997206/range1997206/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165089/annotation1997206/range1997206/1
type: Range
persistent identity: range1997206
display id: range1997206
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 1
end: 127
orientation: inline

BBa_K165020
type: SequenceAnnotation
persistent identity: annotation1997208
display id: annotation1997208
version: 1
top level: BBa_K165089
location: https://synbiohub.org/public/igem/BBa_K165089/annotation1997208/range1997208/1
component: BBa_K165020

https://synbiohub.org/public/igem/BBa_K165089/annotation1997208/range1997208/1
type: Range
persistent identity: range1997208
display id: range1997208
version: 1
direction: direction/reverse
top level: BBa_K165089
start: 547
end: 1218
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K105010
display id: BBa_K105010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Yeast optimized for fusion proteins
creator: Marius M??ller, Paul Bourgine
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is red fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of a BioBrick part for fusion proteins (see design details). <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of BBa_E2060 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105010_sequence/1

BBa_K1871010
type: ComponentDefinition
persistent identity: BBa K1871010
display id: BBa_K1871010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence AMY1
creator: Broad Run High School iGEM Team
created: 2015-09-12T11:00:00Z
modified: 2016-10-15T09:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2303_
m user id: 27970
owner id: 28667
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is an alpha amylase gene obtained from Bacillus amyloliquefaciens. It is flanked by a Kozak sequence (Part BBa_K165002) and ADH1 terminator (Part BBa_K392003). The construct is contained in the pSB1C3 plasmid.
mutable notes: TBD
mutable provenance: This came from the published sequence for Bacillus amyloliquefaciens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Kozak Sequence
sequence annotation: Alpha Amylase Coding Sequence
sequence annotation: ADH1 Terminator
sequence annotation: Native Alpha Amylase Secretion Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1871010_sequence/1

Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2462517
display id: annotation2462517
version: 1
top level: BBa_K1871010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1871010/annotation2462517/range2462517/1

https://synbiohub.org/public/igem/BBa_K1871010/annotation2462517/range2462517/1
type: Range
persistent identity: range2462517
display id: range2462517
version: 1
direction: direction/forward
top level: BBa_K1871010
start: 8
end: 25
orientation: inline

Alpha Amylase Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2462535
display id: annotation2462535
version: 1
top level: BBa_K1871010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1871010/annotation2462535/range2462535/1

https://synbiohub.org/public/igem/BBa_K1871010/annotation2462535/range2462535/1
type: Range
persistent identity: range2462535
display id: range2462535
version: 1
direction: direction/forward
top level: BBa_K1871010
start: 119
end: 1570
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2462553
display id: annotation2462553
version: 1
top level: BBa_K1871010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1871010/annotation2462553/range2462553/1

https://synbiohub.org/public/igem/BBa_K1871010/annotation2462553/range2462553/1
type: Range
persistent identity: range2462553
display id: range2462553
version: 1
direction: direction/forward
top level: BBa_K1871010
start: 1571
end: 1699
orientation: inline

Native Alpha Amylase Secretion Sequence
type: SequenceAnnotation
persistent identity: annotation2462533
display id: annotation2462533
version: 1
top level: BBa_K1871010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1871010/annotation2462533/range2462533/1

https://synbiohub.org/public/igem/BBa_K1871010/annotation2462533/range2462533/1
type: Range
persistent identity: range2462533
display id: range2462533
version: 1
direction: direction/forward
top level: BBa_K1871010
start: 26
end: 118
orientation: inline

BBa_K976006
type: ComponentDefinition
persistent identity: BBa K976006
display id: BBa_K976006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast ADH1 promoter + Firefly Luciferase
creator: Amna Sohail & Molly Petrous & Tyler Knox & Mackenzie Reetz
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15159
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Yeast promoter + luciferase composite part
mutable notes: No design considerations
mutable provenance: NBCI Gen Bank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J63005
component: BBa_I712019
sequence annotation: BBa_J63005
sequence annotation: BBa_I712019
sequence: https://synbiohub.org/public/igem/BBa_K976006_sequence/1

BBa_J63005
type: Component
persistent identity: component2329810
display id: component2329810
version: 1
top level: BBa_K976006
access: public
definition: P0310

BBa_I712019
type: Component
persistent identity: component2329811
display id: component2329811
version: 1
top level: BBa_K976006
access: public
definition: https://synbiohub.org/public/igem/BBa_I712019/1

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2329810
display id: annotation2329810
version: 1
top level: BBa_K976006
location: https://synbiohub.org/public/igem/BBa_K976006/annotation2329810/range2329810/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K976006/annotation2329810/range2329810/1
type: Range
persistent identity: range2329810
display id: range2329810
version: 1
direction: direction/reverse
top level: BBa_K976006
start: 1
end: 1445
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2329811
display id: annotation2329811
version: 1
top level: BBa_K976006
location: https://synbiohub.org/public/igem/BBa_K976006/annotation2329811/range2329811/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K976006/annotation2329811/range2329811/1
type: Range
persistent identity: range2329811
display id: range2329811
version: 1
direction: direction/reverse
top level: BBa_K976006
start: 1452
end: 3104
orientation: inline

BBa_K319043
type: ComponentDefinition
persistent identity: BBa K319043
display id: BBa_K319043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADE4 targeting vector
creator: Afnan Azizi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype.
mutable notes: N/A
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Cm resistance
sequence annotation: Forward landing pad
sequence annotation: EcoRI
sequence annotation: PstI site
sequence annotation: PstI/NsiI mixed site
sequence annotation: ADE4 terminator
sequence annotation: EcoRI/MfeI mixed site
sequence annotation: G5C5G5 filler site
sequence annotation: Reverse landing pad
sequence annotation: ATM1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K319043_sequence/1

Cm resistance
type: SequenceAnnotation
persistent identity: annotation2109321
display id: annotation2109321
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109321/range2109321/1
type: Range
persistent identity: range2109321
display id: range2109321
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 1620
end: 2279
orientation: inline

Forward landing pad
type: SequenceAnnotation
persistent identity: annotation2109296
display id: annotation2109296
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109296/range2109296/1
type: Range
persistent identity: range2109296
display id: range2109296
version: 1
direction: direction/forward
top level: BBa_K319043
start: 207
end: 226
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2109293
display id: annotation2109293
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109293/range2109293/1
type: Range
persistent identity: range2109293
display id: range2109293
version: 1
direction: direction/forward
top level: BBa_K319043
start: 227
end: 232
orientation: inline

PstI site
type: SequenceAnnotation
persistent identity: annotation2109295
display id: annotation2109295
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109295/range2109295/1
type: Range
persistent identity: range2109295
display id: range2109295
version: 1
direction: direction/forward
top level: BBa_K319043
start: 248
end: 253
orientation: inline

PstI/NsiI mixed site
type: SequenceAnnotation
persistent identity: annotation2109301
display id: annotation2109301
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109301/range2109301/1
type: Range
persistent identity: range2109301
display id: range2109301
version: 1
direction: direction/forward
top level: BBa_K319043
start: 474
end: 479
orientation: inline

ADE4 terminator
type: SequenceAnnotation
persistent identity: annotation2109298
display id: annotation2109298
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109298/range2109298/1
type: Range
persistent identity: range2109298
display id: range2109298
version: 1
direction: direction/forward
top level: BBa_K319043
start: 274
end: 473
orientation: inline

EcoRI/MfeI mixed site
type: SequenceAnnotation
persistent identity: annotation2109290
display id: annotation2109290
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109290/range2109290/1
type: Range
persistent identity: range2109290
display id: range2109290
version: 1
direction: direction/forward
top level: BBa_K319043
start: 1
end: 6
orientation: inline

G5C5G5 filler site
type: SequenceAnnotation
persistent identity: annotation2109294
display id: annotation2109294
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109294/range2109294/1
type: Range
persistent identity: range2109294
display id: range2109294
version: 1
direction: direction/forward
top level: BBa_K319043
start: 233
end: 247
orientation: inline

Reverse landing pad
type: SequenceAnnotation
persistent identity: annotation2109297
display id: annotation2109297
version: 1
top level: BBa_K319043
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109297/range2109297/1
type: Range
persistent identity: range2109297
display id: range2109297
version: 1
direction: direction/reverse
top level: BBa_K319043
start: 254
end: 273
orientation: inline

ATM1 terminator
type: SequenceAnnotation
persistent identity: annotation2109292
display id: annotation2109292
version: 1
top level: BBa_K319043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1

https://synbiohub.org/public/igem/BBa_K319043/annotation2109292/range2109292/1
type: Range
persistent identity: range2109292
display id: range2109292
version: 1
direction: direction/forward
top level: BBa_K319043
start: 7
end: 206
orientation: inline

BBa_K1314017
type: ComponentDefinition
persistent identity: BBa K1314017
display id: BBa_K1314017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mid1 (S. cerevisae Codon Opt)
creator: CHIU, Yee Ting
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
group u list: _1689_
m user id: 0
owner id: 22040
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mid1 is a voltage gated Calcium channel(VGCC) of yeast which works with Cch1, another VGCC. It was codon optimized for S.cerevisae
mutable notes: With biobrick prefix and suffix added and illegal site eliminated.
mutable provenance: Synthesized by IDT Ltd.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Mid1
sequence annotation: HA tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1314017_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2398389
display id: annotation2398389
version: 1
top level: BBa_K1314017
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1314017/annotation2398389/range2398389/1

https://synbiohub.org/public/igem/BBa_K1314017/annotation2398389/range2398389/1
type: Range
persistent identity: range2398389
display id: range2398389
version: 1
direction: direction/forward
top level: BBa_K1314017
start: 1
end: 3
orientation: inline

Mid1
type: SequenceAnnotation
persistent identity: annotation2398390
display id: annotation2398390
version: 1
top level: BBa_K1314017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1314017/annotation2398390/range2398390/1

https://synbiohub.org/public/igem/BBa_K1314017/annotation2398390/range2398390/1
type: Range
persistent identity: range2398390
display id: range2398390
version: 1
direction: direction/forward
top level: BBa_K1314017
start: 1
end: 1673
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2398387
display id: annotation2398387
version: 1
top level: BBa_K1314017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1314017/annotation2398387/range2398387/1

https://synbiohub.org/public/igem/BBa_K1314017/annotation2398387/range2398387/1
type: Range
persistent identity: range2398387
display id: range2398387
version: 1
direction: direction/forward
top level: BBa_K1314017
start: 4
end: 33
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2398388
display id: annotation2398388
version: 1
top level: BBa_K1314017
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1314017/annotation2398388/range2398388/1

https://synbiohub.org/public/igem/BBa_K1314017/annotation2398388/range2398388/1
type: Range
persistent identity: range2398388
display id: range2398388
version: 1
direction: direction/forward
top level: BBa_K1314017
start: 1674
end: 1680
orientation: inline

BBa_E2030
type: ComponentDefinition
persistent identity: BBa E2030
display id: BBa_E2030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of EYFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Venus YFP, yeast codon optimized. EX515/EM528
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: nagai T. et al. (2002) Nat Biot (20):87-90
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: MATSG linker
sequence annotation: Venus YFP
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2030_sequence/1

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431979
display id: annotation1431979
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1
type: Range
persistent identity: range1431979
display id: range1431979
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 15
orientation: inline

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1431978
display id: annotation1431978
version: 1
top level: BBa_E2030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1
type: Range
persistent identity: range1431978
display id: range1431978
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 747
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431980
display id: annotation1431980
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1
type: Range
persistent identity: range1431980
display id: range1431980
version: 1
direction: direction/forward
top level: BBa_E2030
start: 733
end: 747
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214039
display id: annotation2214039
version: 1
top level: BBa_E2030
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1

https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1
type: Range
persistent identity: range2214039
display id: range2214039
version: 1
direction: direction/forward
top level: BBa_E2030
start: 754
end: 778
orientation: inline

BBa_K1611000
type: ComponentDefinition
persistent identity: BBa K1611000
display id: BBa_K1611000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFNgamma
creator: Frederic Ros
created: 2015-09-14T11:00:00Z
modified: 2015-12-17T01:03:34Z
discontinued: false
dominant: false
group u list: _2028_
m user id: 4206
owner id: 28291
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory.
mutable notes: We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.
mutable provenance: This sequence was synthesised by IDT based on the genomic murine IFN gamma.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop
sequence annotation: start
sequence annotation: c.198G>C
sequence: https://synbiohub.org/public/igem/BBa_K1611000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2456822
display id: annotation2456822
version: 1
top level: BBa_K1611000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1611000/annotation2456822/range2456822/1

https://synbiohub.org/public/igem/BBa_K1611000/annotation2456822/range2456822/1
type: Range
persistent identity: range2456822
display id: range2456822
version: 1
direction: direction/forward
top level: BBa_K1611000
start: 1
end: 468
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2456820
display id: annotation2456820
version: 1
top level: BBa_K1611000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1611000/annotation2456820/range2456820/1

https://synbiohub.org/public/igem/BBa_K1611000/annotation2456820/range2456820/1
type: Range
persistent identity: range2456820
display id: range2456820
version: 1
direction: direction/forward
top level: BBa_K1611000
start: 466
end: 471
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2456821
display id: annotation2456821
version: 1
top level: BBa_K1611000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1611000/annotation2456821/range2456821/1

https://synbiohub.org/public/igem/BBa_K1611000/annotation2456821/range2456821/1
type: Range
persistent identity: range2456821
display id: range2456821
version: 1
direction: direction/forward
top level: BBa_K1611000
start: 1
end: 3
orientation: inline

c.198G>C
type: SequenceAnnotation
persistent identity: annotation2456819
display id: annotation2456819
version: 1
top level: BBa_K1611000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1611000/annotation2456819/range2456819/1

https://synbiohub.org/public/igem/BBa_K1611000/annotation2456819/range2456819/1
type: Range
persistent identity: range2456819
display id: range2456819
version: 1
direction: direction/forward
top level: BBa_K1611000
start: 198
end: 198
orientation: inline

MlrAa
type: ComponentDefinition
persistent identity: BBa K1907006
display id: BBa_K1907006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Microcystinase (Mlra) + mating factor alpha tag, for S. cerevisiae
creator: Pihla Savola
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T01:33:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29559
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: Mating alpha tag
sequence annotation: MlrA
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K1907006_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2522192
display id: annotation2522192
version: 1
top level: MlrAa
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1907006/annotation2522192/range2522192/1

https://synbiohub.org/public/igem/BBa_K1907006/annotation2522192/range2522192/1
type: Range
persistent identity: range2522192
display id: range2522192
version: 1
direction: direction/forward
top level: MlrAa
start: 1276
end: 1284
orientation: inline

Mating alpha tag
type: SequenceAnnotation
persistent identity: annotation2522189
display id: annotation2522189
version: 1
top level: MlrAa
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1907006/annotation2522189/range2522189/1

https://synbiohub.org/public/igem/BBa_K1907006/annotation2522189/range2522189/1
type: Range
persistent identity: range2522189
display id: range2522189
version: 1
direction: direction/forward
top level: MlrAa
start: 1
end: 267
orientation: inline

MlrA
type: SequenceAnnotation
persistent identity: annotation2522196
display id: annotation2522196
version: 1
top level: MlrAa
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1907006/annotation2522196/range2522196/1

https://synbiohub.org/public/igem/BBa_K1907006/annotation2522196/range2522196/1
type: Range
persistent identity: range2522196
display id: range2522196
version: 1
direction: direction/forward
top level: MlrAa
start: 268
end: 1275
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2522210
display id: annotation2522210
version: 1
top level: MlrAa
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1907006/annotation2522210/range2522210/1

https://synbiohub.org/public/igem/BBa_K1907006/annotation2522210/range2522210/1
type: Range
persistent identity: range2522210
display id: range2522210
version: 1
direction: direction/forward
top level: MlrAa
start: 1285
end: 1302
orientation: inline

BBa_K1611004
type: ComponentDefinition
persistent identity: BBa K1611004
display id: BBa_K1611004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DEC-205
creator: Frederic Ros
created: 2015-09-14T11:00:00Z
modified: 2015-12-17T01:06:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2028_
m user id: 4206
owner id: 28291
owning group id: 9
status: status/Planning
bookmark: false
mutable description: DEC-205 is an antigen against an endocytic receptor that is expressed by dendritic cells. Antigen endocytosed via CD205 enters the MHC class I and MHC class II antigen presentation pathways and is subsequently presented to both CD4+ and CD8+ T cells. We used it to activate T-CD8 cells, with tumorous antigen that we binded to.
mutable notes: Nothing to signal.
mutable provenance: Edit source.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1611004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2456862
display id: annotation2456862
version: 1
top level: BBa_K1611004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1611004/annotation2456862/range2456862/1

https://synbiohub.org/public/igem/BBa_K1611004/annotation2456862/range2456862/1
type: Range
persistent identity: range2456862
display id: range2456862
version: 1
direction: direction/forward
top level: BBa_K1611004
start: 796
end: 801
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2456860
display id: annotation2456860
version: 1
top level: BBa_K1611004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1611004/annotation2456860/range2456860/1

https://synbiohub.org/public/igem/BBa_K1611004/annotation2456860/range2456860/1
type: Range
persistent identity: range2456860
display id: range2456860
version: 1
direction: direction/forward
top level: BBa_K1611004
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2456861
display id: annotation2456861
version: 1
top level: BBa_K1611004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1611004/annotation2456861/range2456861/1

https://synbiohub.org/public/igem/BBa_K1611004/annotation2456861/range2456861/1
type: Range
persistent identity: range2456861
display id: range2456861
version: 1
direction: direction/forward
top level: BBa_K1611004
start: 1
end: 798
orientation: inline

BBa_K1936001
type: ComponentDefinition
persistent identity: BBa K1936001
display id: BBa_K1936001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tom5-eGFP-LOV2
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker
sequence annotation: eGFP
sequence annotation: TOM5
sequence annotation: LOV2
sequence: https://synbiohub.org/public/igem/BBa_K1936001_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2521538
display id: annotation2521538
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1
type: Range
persistent identity: range2521538
display id: range2521538
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 154
end: 192
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521540
display id: annotation2521540
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1
type: Range
persistent identity: range2521540
display id: range2521540
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 907
end: 936
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2521539
display id: annotation2521539
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1
type: Range
persistent identity: range2521539
display id: range2521539
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 193
end: 906
orientation: inline

TOM5
type: SequenceAnnotation
persistent identity: annotation2521537
display id: annotation2521537
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1
type: Range
persistent identity: range2521537
display id: range2521537
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 1
end: 153
orientation: inline

LOV2
type: SequenceAnnotation
persistent identity: annotation2521541
display id: annotation2521541
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1
type: Range
persistent identity: range2521541
display id: range2521541
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 937
end: 1368
orientation: inline

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak sequence (yeast RBS)
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

BBa_I766556
type: ComponentDefinition
persistent identity: BBa I766556
display id: BBa_I766556
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAdh (Strong) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong Expression level Constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Alcohol Dehydrogenase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766556_sequence/1

BBa_K801040
type: ComponentDefinition
persistent identity: BBa K801040
display id: BBa_K801040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K801032
sequence annotation: BBa_K801038
sequence annotation: BBa_K365005
sequence annotation: BBa_K801037
sequence annotation: BBa_K801031
sequence annotation: BBa_K801030
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: RFC25 fusion scar
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence: https://synbiohub.org/public/igem/BBa_K801040_sequence/1

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2199590
display id: annotation2199590
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1
type: Range
persistent identity: range2199590
display id: range2199590
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2857
end: 3294
orientation: inline

BBa_K801038
type: SequenceAnnotation
persistent identity: annotation2199591
display id: annotation2199591
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1
type: Range
persistent identity: range2199591
display id: range2199591
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 3294
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199587
display id: annotation2199587
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1
type: Range
persistent identity: range2199587
display id: range2199587
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 2850
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2199588
display id: annotation2199588
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1
type: Range
persistent identity: range2199588
display id: range2199588
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 3294
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199580
display id: annotation2199580
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1
type: Range
persistent identity: range2199580
display id: range2199580
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 2784
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199579
display id: annotation2199579
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1
type: Range
persistent identity: range2199579
display id: range2199579
version: 1
direction: direction/reverse
top level: BBa_K801040
start: 1
end: 57
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199583
display id: annotation2199583
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1
type: Range
persistent identity: range2199583
display id: range2199583
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1102
end: 1104
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199581
display id: annotation2199581
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1
type: Range
persistent identity: range2199581
display id: range2199581
version: 1
direction: direction/forward
top level: BBa_K801040
start: 388
end: 390
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199589
display id: annotation2199589
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1
type: Range
persistent identity: range2199589
display id: range2199589
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2851
end: 2856
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199578
display id: annotation2199578
version: 1
top level: BBa_K801040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1
type: Range
persistent identity: range2199578
display id: range2199578
version: 1
direction: direction/forward
top level: BBa_K801040
start: 22
end: 42
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199586
display id: annotation2199586
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1
type: Range
persistent identity: range2199586
display id: range2199586
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2785
end: 2790
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199585
display id: annotation2199585
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1
type: Range
persistent identity: range2199585
display id: range2199585
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1129
end: 1131
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199584
display id: annotation2199584
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1
type: Range
persistent identity: range2199584
display id: range2199584
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1114
end: 1116
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199582
display id: annotation2199582
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1
type: Range
persistent identity: range2199582
display id: range2199582
version: 1
direction: direction/forward
top level: BBa_K801040
start: 391
end: 393
orientation: inline

BBa_K392005
type: ComponentDefinition
persistent identity: BBa K392005
display id: BBa_K392005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast SUC2 leader sequence
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LacI promoter with RBS, followed by OmpA outer membrane protein and linker for Escherichia coli cell surface display.
mutable notes: Assembled by 3A method.
mutable provenance: Composite part BBa_K392001 (LacI promoter + RBS) and registry part BBa_K103006 (OmpA w/ linker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K392005_sequence/1

BBa_K555009
type: ComponentDefinition
persistent identity: BBa K555009
display id: BBa_K555009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pYES2 - galactose-inducible expression vector for yeast
creator: Meghan Savage
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _723_
m user id: 0
owner id: 9577
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pYES2 yeast vector
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K555009_sequence/1

BBa_K801095
type: ComponentDefinition
persistent identity: BBa K801095
display id: BBa_K801095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin - chalcone synthase (CHS) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Naringenin - chalcone synthase (CHS) coding region from ''Hypericum androsaemum''
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/11096318/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: CHS
sequence: https://synbiohub.org/public/igem/BBa_K801095_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202152
display id: annotation2202152
version: 1
top level: BBa_K801095
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801095/annotation2202152/range2202152/1

https://synbiohub.org/public/igem/BBa_K801095/annotation2202152/range2202152/1
type: Range
persistent identity: range2202152
display id: range2202152
version: 1
direction: direction/forward
top level: BBa_K801095
start: 1
end: 3
orientation: inline

CHS
type: SequenceAnnotation
persistent identity: annotation2202163
display id: annotation2202163
version: 1
top level: BBa_K801095
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801095/annotation2202163/range2202163/1

https://synbiohub.org/public/igem/BBa_K801095/annotation2202163/range2202163/1
type: Range
persistent identity: range2202163
display id: range2202163
version: 1
direction: direction/forward
top level: BBa_K801095
start: 1
end: 1170
orientation: inline

BBa_K1072020
type: ComponentDefinition
persistent identity: BBa K1072020
display id: BBa_K1072020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FUS1+BFP+ADH1
creator: Xihao liao
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072023
component: BBa_K801012
component: BBa_K592009
sequence annotation: BBa_K801012
sequence annotation: BBa_K592009
sequence annotation: BBa_K1072023
sequence: https://synbiohub.org/public/igem/BBa_K1072020_sequence/1

BBa_K1072023
type: Component
persistent identity: component2349223
display id: component2349223
version: 1
top level: BBa_K1072020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072023/1

BBa_K801012
type: Component
persistent identity: component2349227
display id: component2349227
version: 1
top level: BBa_K1072020
access: public
definition: BBa_K801012

BBa_K592009
type: Component
persistent identity: component2349225
display id: component2349225
version: 1
top level: BBa_K1072020
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2349227
display id: annotation2349227
version: 1
top level: BBa_K1072020
location: https://synbiohub.org/public/igem/BBa_K1072020/annotation2349227/range2349227/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1072020/annotation2349227/range2349227/1
type: Range
persistent identity: range2349227
display id: range2349227
version: 1
direction: direction/reverse
top level: BBa_K1072020
start: 1122
end: 1470
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2349225
display id: annotation2349225
version: 1
top level: BBa_K1072020
location: https://synbiohub.org/public/igem/BBa_K1072020/annotation2349225/range2349225/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1072020/annotation2349225/range2349225/1
type: Range
persistent identity: range2349225
display id: range2349225
version: 1
direction: direction/reverse
top level: BBa_K1072020
start: 445
end: 1113
orientation: inline

BBa_K1072023
type: SequenceAnnotation
persistent identity: annotation2349223
display id: annotation2349223
version: 1
top level: BBa_K1072020
location: https://synbiohub.org/public/igem/BBa_K1072020/annotation2349223/range2349223/1
component: BBa_K1072023

https://synbiohub.org/public/igem/BBa_K1072020/annotation2349223/range2349223/1
type: Range
persistent identity: range2349223
display id: range2349223
version: 1
direction: direction/reverse
top level: BBa_K1072020
start: 1
end: 438
orientation: inline

BBa_I766557
type: ComponentDefinition
persistent identity: BBa I766557
display id: BBa_I766557
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte5 (Weak) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: low expression, constitutive promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Sterile 5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766557_sequence/1

BBa_K801091
type: ComponentDefinition
persistent identity: BBa K801091
display id: BBa_K801091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phenylalanine ammonia lyase (PAL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the enzyme phenylalanine ammonia lyase (PAL) from ''Rhodosporidium toruloides''
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nuccore/AX366866 PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Phe instead of Ser
sequence annotation: Deletion of Cys
sequence annotation: Start codon
sequence annotation: PAL
sequence annotation: Mutation
sequence: https://synbiohub.org/public/igem/BBa_K801091_sequence/1

Phe instead of Ser
type: SequenceAnnotation
persistent identity: annotation2202137
display id: annotation2202137
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202137/range2202137/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202137/range2202137/1
type: Range
persistent identity: range2202137
display id: range2202137
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1364
end: 1364
orientation: inline

Deletion of Cys
type: SequenceAnnotation
persistent identity: annotation2202133
display id: annotation2202133
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202133/range2202133/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202133/range2202133/1
type: Range
persistent identity: range2202133
display id: range2202133
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1358
end: 1360
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202128
display id: annotation2202128
version: 1
top level: BBa_K801091
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202128/range2202128/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202128/range2202128/1
type: Range
persistent identity: range2202128
display id: range2202128
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1
end: 3
orientation: inline

PAL
type: SequenceAnnotation
persistent identity: annotation2202130
display id: annotation2202130
version: 1
top level: BBa_K801091
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202130/range2202130/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202130/range2202130/1
type: Range
persistent identity: range2202130
display id: range2202130
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1
end: 2148
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2202129
display id: annotation2202129
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202129/range2202129/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202129/range2202129/1
type: Range
persistent identity: range2202129
display id: range2202129
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1356
end: 1356
orientation: inline

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast GAL1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GAL1 promoter from S. cerevisiae
mutable notes: unchanged from genomic sequence
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_K801062
type: ComponentDefinition
persistent identity: BBa K801062
display id: BBa_K801062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express Citrus limon (+)-limonene synthase 1 in yeast. The expression is controled by the TEF1 promoter and the CYC1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the ''Citrus limon'' (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K801061
sequence annotation: Cyc terminator
sequence annotation: Stop codon
sequence annotation: Coding sequence
sequence annotation: TEF1 promotor
sequence: https://synbiohub.org/public/igem/BBa_K801062_sequence/1

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197879
display id: annotation2197879
version: 1
top level: BBa_K801062
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197879/range2197879/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197879/range2197879/1
type: Range
persistent identity: range2197879
display id: range2197879
version: 1
direction: direction/forward
top level: BBa_K801062
start: 462
end: 2126
orientation: inline

Cyc terminator
type: SequenceAnnotation
persistent identity: annotation2197884
display id: annotation2197884
version: 1
top level: BBa_K801062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197884/range2197884/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197884/range2197884/1
type: Range
persistent identity: range2197884
display id: range2197884
version: 1
direction: direction/forward
top level: BBa_K801062
start: 2143
end: 2424
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2197885
display id: annotation2197885
version: 1
top level: BBa_K801062
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197885/range2197885/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197885/range2197885/1
type: Range
persistent identity: range2197885
display id: range2197885
version: 1
direction: direction/forward
top level: BBa_K801062
start: 2133
end: 2137
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197878
display id: annotation2197878
version: 1
top level: BBa_K801062
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197878/range2197878/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197878/range2197878/1
type: Range
persistent identity: range2197878
display id: range2197878
version: 1
direction: direction/forward
top level: BBa_K801062
start: 462
end: 2126
orientation: inline

TEF1 promotor
type: SequenceAnnotation
persistent identity: annotation2197883
display id: annotation2197883
version: 1
top level: BBa_K801062
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197883/range2197883/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197883/range2197883/1
type: Range
persistent identity: range2197883
display id: range2197883
version: 1
direction: direction/forward
top level: BBa_K801062
start: 1
end: 457
orientation: inline

BBa_K165015
type: ComponentDefinition
persistent identity: BBa K165015
display id: BBa_K165015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH1 yeast constituative promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a low-level constitutive yeast promoter.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165015_sequence/1

BBa_K1592000
type: ComponentDefinition
persistent identity: BBa K1592000
display id: BBa_K1592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LIP2 prepro(signal peptide)
creator: Shuyan Tang
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T09:23:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2009_
m user id: 20267
owner id: 20267
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The pre-region of lipase 2 from Yarrowia lipolytica corresponds to the signal sequence; the dipeptides XA and XP are substrates for diamino-peptidase; the dibasic KR cleavage site is substrate for Xpr6p endoproteinase.
mutable notes: No.
mutable provenance: It have been contributed from our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KR cleavage site
sequence annotation: 4 XA or XP
sequence annotation: LIP2 pro
sequence annotation: Start codon
sequence annotation: LIP2 pre
sequence: https://synbiohub.org/public/igem/BBa_K1592000_sequence/1

KR cleavage site
type: SequenceAnnotation
persistent identity: annotation2444448
display id: annotation2444448
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444448/range2444448/1
type: Range
persistent identity: range2444448
display id: range2444448
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 94
end: 99
orientation: inline

4 XA or XP
type: SequenceAnnotation
persistent identity: annotation2444446
display id: annotation2444446
version: 1
top level: BBa_K1592000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444446/range2444446/1
type: Range
persistent identity: range2444446
display id: range2444446
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 40
end: 63
orientation: inline

LIP2 pro
type: SequenceAnnotation
persistent identity: annotation2444447
display id: annotation2444447
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444447/range2444447/1
type: Range
persistent identity: range2444447
display id: range2444447
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 64
end: 93
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2455904
display id: annotation2455904
version: 1
top level: BBa_K1592000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2455904/range2455904/1
type: Range
persistent identity: range2455904
display id: range2455904
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 3
orientation: inline

LIP2 pre
type: SequenceAnnotation
persistent identity: annotation2444445
display id: annotation2444445
version: 1
top level: BBa_K1592000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1

https://synbiohub.org/public/igem/BBa_K1592000/annotation2444445/range2444445/1
type: Range
persistent identity: range2444445
display id: range2444445
version: 1
direction: direction/forward
top level: BBa_K1592000
start: 1
end: 39
orientation: inline

BBa_K801081
type: ComponentDefinition
persistent identity: BBa K801081
display id: BBa_K801081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prepro-Thaumatin expression cassette for yeast
creator: Alois Br??uer
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part can be used to constitutively express Prepro-Thaumatin in ''S. cerevisiae'' under the control of the TEF1-promoter and the TEF1-terminator.
mutable notes: -
mutable provenance: Based on part BBa_K801080 (Prepro-Thaumatin).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K801011
component: BBa_K319003
component: BBa_K801080
sequence annotation: BBa_K319003
sequence annotation: BBa_K801011
sequence annotation: BBa_K801080
sequence: https://synbiohub.org/public/igem/BBa_K801081_sequence/1

BBa_K801011
type: Component
persistent identity: component2373056
display id: component2373056
version: 1
top level: BBa_K801081
access: public
definition: BBa_K801011

BBa_K319003
type: Component
persistent identity: component2373050
display id: component2373050
version: 1
top level: BBa_K801081
access: public
definition: https://synbiohub.org/public/igem/BBa_K319003/1

BBa_K801080
type: Component
persistent identity: component2373054
display id: component2373054
version: 1
top level: BBa_K801081
access: public
definition: BBa_K801080

BBa_K319003
type: SequenceAnnotation
persistent identity: annotation2373050
display id: annotation2373050
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373050/range2373050/1
component: BBa_K319003

https://synbiohub.org/public/igem/BBa_K801081/annotation2373050/range2373050/1
type: Range
persistent identity: range2373050
display id: range2373050
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 1
end: 450
orientation: inline

BBa_K801011
type: SequenceAnnotation
persistent identity: annotation2373056
display id: annotation2373056
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373056/range2373056/1
component: BBa_K801011

https://synbiohub.org/public/igem/BBa_K801081/annotation2373056/range2373056/1
type: Range
persistent identity: range2373056
display id: range2373056
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 1173
end: 1679
orientation: inline

BBa_K801080
type: SequenceAnnotation
persistent identity: annotation2373054
display id: annotation2373054
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373054/range2373054/1
component: BBa_K801080

https://synbiohub.org/public/igem/BBa_K801081/annotation2373054/range2373054/1
type: Range
persistent identity: range2373054
display id: range2373054
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 457
end: 1164
orientation: inline

BBa_K165016
type: ComponentDefinition
persistent identity: BBa K165016
display id: BBa_K165016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC1 minimal yeast promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165016_sequence/1

BBa_K394001
type: ComponentDefinition
persistent identity: BBa K394001
display id: BBa_K394001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at His3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the His3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains His3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using His- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR (rev)
sequence annotation: His3 homology
sequence annotation: BioBrick Prefix
sequence annotation: rep (pMB1) (rev)
sequence annotation: BbsI Cut Site
sequence annotation: VF2
sequence annotation: Tet-R
sequence annotation: BioBrick Suffix
sequence annotation: ORI
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K394001_sequence/1

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102638
display id: annotation2102638
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102638/range2102638/1
type: Range
persistent identity: range2102638
display id: range2102638
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 2324
end: 3184
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102389
display id: annotation2102389
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102389/range2102389/1
type: Range
persistent identity: range2102389
display id: range2102389
version: 1
direction: direction/forward
top level: BBa_K394001
start: 22
end: 69
orientation: inline

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102649
display id: annotation2102649
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102649/range2102649/1
type: Range
persistent identity: range2102649
display id: range2102649
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3435
end: 3457
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102598
display id: annotation2102598
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102598/range2102598/1
type: Range
persistent identity: range2102598
display id: range2102598
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 261
end: 875
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104026
display id: annotation2104026
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104026/range2104026/1
type: Range
persistent identity: range2104026
display id: range2104026
version: 1
direction: direction/forward
top level: BBa_K394001
start: 71
end: 76
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102640
display id: annotation2102640
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102640/range2102640/1
type: Range
persistent identity: range2102640
display id: range2102640
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3307
end: 3326
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102637
display id: annotation2102637
version: 1
top level: BBa_K394001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102637/range2102637/1
type: Range
persistent identity: range2102637
display id: range2102637
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1127
end: 2317
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104028
display id: annotation2104028
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2104028/range2104028/1
type: Range
persistent identity: range2104028
display id: range2104028
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 3379
end: 3385
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2090942
display id: annotation2090942
version: 1
top level: BBa_K394001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2090942/range2090942/1
type: Range
persistent identity: range2090942
display id: range2090942
version: 1
direction: direction/forward
top level: BBa_K394001
start: 1
end: 21
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102635
display id: annotation2102635
version: 1
top level: BBa_K394001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102635/range2102635/1
type: Range
persistent identity: range2102635
display id: range2102635
version: 1
direction: direction/forward
top level: BBa_K394001
start: 276
end: 276
orientation: inline

His3 homology
type: SequenceAnnotation
persistent identity: annotation2102645
display id: annotation2102645
version: 1
top level: BBa_K394001
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102645/range2102645/1
type: Range
persistent identity: range2102645
display id: range2102645
version: 1
direction: direction/forward
top level: BBa_K394001
start: 3386
end: 3435
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102390
display id: annotation2102390
version: 1
top level: BBa_K394001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1

https://synbiohub.org/public/igem/BBa_K394001/annotation2102390/range2102390/1
type: Range
persistent identity: range2102390
display id: range2102390
version: 1
direction: direction/reverse
top level: BBa_K394001
start: 157
end: 176
orientation: inline

PKI NES
type: ComponentDefinition
persistent identity: BBa J63007
display id: BBa_J63007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PKI nuclear export sequence; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: yeast codon optimized PKI nuclear export sequence
mutable notes: optimized codons for S. cerevisiae
mutable provenance: from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63007_sequence/1

Cripto-1
type: ComponentDefinition
persistent identity: BBa K1908001
display id: BBa_K1908001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for Homo sapien Cripto-1 membrane protein.
creator: Ryan Kawalerski, Tasnia Islam, Joshua Hombrebueno, Sarah Heacox, Samara Khan, Daniel Thach, Meghan Bialt deCelie.
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T10:51:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1908001
sequence annotation: ADH1 Terminator
sequence annotation: stop
sequence annotation: S. Cerevisiae Kozak Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1908001_sequence/1

BBa_K1908001
type: SequenceAnnotation
persistent identity: annotation2511513
display id: annotation2511513
version: 1
top level: Cripto-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2511513/range2511513/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2511513/range2511513/1
type: Range
persistent identity: range2511513
display id: range2511513
version: 1
direction: direction/forward
top level: Cripto-1
start: 10
end: 573
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2520178
display id: annotation2520178
version: 1
top level: Cripto-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520178/range2520178/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520178/range2520178/1
type: Range
persistent identity: range2520178
display id: range2520178
version: 1
direction: direction/forward
top level: Cripto-1
start: 580
end: 767
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2520174
display id: annotation2520174
version: 1
top level: Cripto-1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520174/range2520174/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520174/range2520174/1
type: Range
persistent identity: range2520174
display id: range2520174
version: 1
direction: direction/forward
top level: Cripto-1
start: 574
end: 579
orientation: inline

S. Cerevisiae Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2520173
display id: annotation2520173
version: 1
top level: Cripto-1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520173/range2520173/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520173/range2520173/1
type: Range
persistent identity: range2520173
display id: range2520173
version: 1
direction: direction/forward
top level: Cripto-1
start: 1
end: 9
orientation: inline

BBa_K1861010
type: ComponentDefinition
persistent identity: BBa K1861010
display id: BBa_K1861010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expression cassette for S. cerevisiae
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T05:30:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K392003
component: BBa_K1861101
component: BBa_K1861103
component: BBa_K1861102
sequence annotation: BBa_K392003
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861103
sequence: https://synbiohub.org/public/igem/BBa_K1861010_sequence/1

BBa_K392003
type: Component
persistent identity: component2471537
display id: component2471537
version: 1
top level: BBa_K1861010
access: public
definition: BBa_K392003

BBa_K1861101
type: Component
persistent identity: component2471534
display id: component2471534
version: 1
top level: BBa_K1861010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861103
type: Component
persistent identity: component2471536
display id: component2471536
version: 1
top level: BBa_K1861010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861103/1

BBa_K1861102
type: Component
persistent identity: component2471535
display id: component2471535
version: 1
top level: BBa_K1861010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2471537
display id: annotation2471537
version: 1
top level: BBa_K1861010
location: https://synbiohub.org/public/igem/BBa_K1861010/annotation2471537/range2471537/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861010/annotation2471537/range2471537/1
type: Range
persistent identity: range2471537
display id: range2471537
version: 1
direction: direction/reverse
top level: BBa_K1861010
start: 314
end: 442
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2471535
display id: annotation2471535
version: 1
top level: BBa_K1861010
location: https://synbiohub.org/public/igem/BBa_K1861010/annotation2471535/range2471535/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861010/annotation2471535/range2471535/1
type: Range
persistent identity: range2471535
display id: range2471535
version: 1
direction: direction/reverse
top level: BBa_K1861010
start: 239
end: 265
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2471534
display id: annotation2471534
version: 1
top level: BBa_K1861010
location: https://synbiohub.org/public/igem/BBa_K1861010/annotation2471534/range2471534/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861010/annotation2471534/range2471534/1
type: Range
persistent identity: range2471534
display id: range2471534
version: 1
direction: direction/reverse
top level: BBa_K1861010
start: 1
end: 238
orientation: inline

BBa_K1861103
type: SequenceAnnotation
persistent identity: annotation2471536
display id: annotation2471536
version: 1
top level: BBa_K1861010
location: https://synbiohub.org/public/igem/BBa_K1861010/annotation2471536/range2471536/1
component: BBa_K1861103

https://synbiohub.org/public/igem/BBa_K1861010/annotation2471536/range2471536/1
type: Range
persistent identity: range2471536
display id: range2471536
version: 1
direction: direction/reverse
top level: BBa_K1861010
start: 266
end: 313
orientation: inline

BBa_K394002
type: ComponentDefinition
persistent identity: BBa K394002
display id: BBa_K394002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid for Chromosomal Integration in Yeast at Ura3
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is designed to allow simple chromosomal integration of BioBrick plasmids. It is based off of the bacterial pSB1AT3 backbone allowing for normal DNA manipulation to occur in bacteria. However the BioBrick cut sites are flanked by homologous regions to the Ura3 locus in S. cerevisiae. To transform S. cerevisiae the plasmid is cut with BbsI creating two non-sticky ends. The linear fragment then contains Ura3 homologous sequences at its ends that can be used to chromosomally integrate the BioBrick plasmid into the yeast genome. Transformants can then be selected for using Ura- medium.
mutable notes: None
mutable provenance: This part was derived from plasmid pSB1AT3. Two successive rounds of PCR inserted a BbsI cut site and 40 bp homologous region in before the prefix and after the suffix. The following Primers were used: Forward 1 GCACAGAACAATAACCTGCTGGAAACGAAGATAAATCgaagacGATTACTTCGCGTTATGCAGGC Reverse 1 GCATCTTCTCAAATATGCTTCCCAGCCTGCTTATCcttctgAAATTCTGCCTCGTGATACGCC Forward 2 tactagtagcggccgctgcagTCTTAACCCAACTGCACAGAACAATAACCTGCTGGAAACG Reverse 2 ctctagaagcggccgcgaattcTTAGTATTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick Prefix
sequence annotation: Ura3 homology
sequence annotation: BbsI Cut Site
sequence annotation: AmpR (rev)
sequence annotation: VF2
sequence annotation: Tet-R
sequence annotation: rep (pMB1) (rev)
sequence annotation: VR
sequence annotation: ORI
sequence annotation: BioBrick Suffix
sequence: https://synbiohub.org/public/igem/BBa_K394002_sequence/1

BioBrick Prefix
type: SequenceAnnotation
persistent identity: annotation2102745
display id: annotation2102745
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102745/range2102745/1
type: Range
persistent identity: range2102745
display id: range2102745
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3435
end: 3457
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102715
display id: annotation2102715
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102715/range2102715/1
type: Range
persistent identity: range2102715
display id: range2102715
version: 1
direction: direction/forward
top level: BBa_K394002
start: 22
end: 69
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2104024
display id: annotation2104024
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2104024/range2104024/1
type: Range
persistent identity: range2104024
display id: range2104024
version: 1
direction: direction/forward
top level: BBa_K394002
start: 72
end: 77
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation2102742
display id: annotation2102742
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102742/range2102742/1
type: Range
persistent identity: range2102742
display id: range2102742
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 2324
end: 3184
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2102743
display id: annotation2102743
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102743/range2102743/1
type: Range
persistent identity: range2102743
display id: range2102743
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3307
end: 3326
orientation: inline

Tet-R
type: SequenceAnnotation
persistent identity: annotation2102741
display id: annotation2102741
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102741/range2102741/1
type: Range
persistent identity: range2102741
display id: range2102741
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1127
end: 2317
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2102717
display id: annotation2102717
version: 1
top level: BBa_K394002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102717/range2102717/1
type: Range
persistent identity: range2102717
display id: range2102717
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 261
end: 875
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2102716
display id: annotation2102716
version: 1
top level: BBa_K394002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102716/range2102716/1
type: Range
persistent identity: range2102716
display id: range2102716
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 157
end: 176
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2102740
display id: annotation2102740
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102740/range2102740/1
type: Range
persistent identity: range2102740
display id: range2102740
version: 1
direction: direction/forward
top level: BBa_K394002
start: 276
end: 276
orientation: inline

BbsI Cut Site
type: SequenceAnnotation
persistent identity: annotation2103940
display id: annotation2103940
version: 1
top level: BBa_K394002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2103940/range2103940/1
type: Range
persistent identity: range2103940
display id: range2103940
version: 1
direction: direction/reverse
top level: BBa_K394002
start: 3381
end: 3386
orientation: inline

BioBrick Suffix
type: SequenceAnnotation
persistent identity: annotation2102714
display id: annotation2102714
version: 1
top level: BBa_K394002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102714/range2102714/1
type: Range
persistent identity: range2102714
display id: range2102714
version: 1
direction: direction/forward
top level: BBa_K394002
start: 1
end: 21
orientation: inline

Ura3 homology
type: SequenceAnnotation
persistent identity: annotation2102744
display id: annotation2102744
version: 1
top level: BBa_K394002
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1

https://synbiohub.org/public/igem/BBa_K394002/annotation2102744/range2102744/1
type: Range
persistent identity: range2102744
display id: range2102744
version: 1
direction: direction/forward
top level: BBa_K394002
start: 3387
end: 3435
orientation: inline

BBa_K165046
type: ComponentDefinition
persistent identity: BBa K165046
display id: BBa_K165046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1+ Kozak
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K165046_sequence/1

pTEF
type: ComponentDefinition
persistent identity: BBa K124001
display id: BBa_K124001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast TEF2 Constitutive Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a mid-high level constitutive promoter from the yeast gene TEF2.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pTEF http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124001_sequence/1

BBa_K110015
type: ComponentDefinition
persistent identity: BBa K110015
display id: BBa_K110015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1 (RtL)
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directlyh from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: MFA1 promoter (RtL)
sequence: https://synbiohub.org/public/igem/BBa_K110015_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981505
display id: annotation1981505
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1
type: Range
persistent identity: range1981505
display id: range1981505
version: 1
direction: direction/forward
top level: BBa_K110015
start: 277
end: 287
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981506
display id: annotation1981506
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1
type: Range
persistent identity: range1981506
display id: range1981506
version: 1
direction: direction/forward
top level: BBa_K110015
start: 302
end: 309
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981504
display id: annotation1981504
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1
type: Range
persistent identity: range1981504
display id: range1981504
version: 1
direction: direction/forward
top level: BBa_K110015
start: 267
end: 276
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981507
display id: annotation1981507
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1
type: Range
persistent identity: range1981507
display id: range1981507
version: 1
direction: direction/forward
top level: BBa_K110015
start: 289
end: 297
orientation: inline

MFA1 promoter (RtL)
type: SequenceAnnotation
persistent identity: annotation1981503
display id: annotation1981503
version: 1
top level: BBa_K110015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1
type: Range
persistent identity: range1981503
display id: range1981503
version: 1
direction: direction/forward
top level: BBa_K110015
start: 1
end: 401
orientation: inline

BBa_K1486023
type: ComponentDefinition
persistent identity: BBa K1486023
display id: BBa_K1486023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Yo sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1486023_sequence/1

Yo sfGFP
type: SequenceAnnotation
persistent identity: annotation2418775
display id: annotation2418775
version: 1
top level: BBa_K1486023
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1

https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1
type: Range
persistent identity: range2418775
display id: range2418775
version: 1
direction: direction/forward
top level: BBa_K1486023
start: 1
end: 717
orientation: inline

Polyargini
type: ComponentDefinition
persistent identity: BBa K792004
display id: BBa_K792004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Import enhancer - Polyarginine (trojan peptide)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell. This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced. (refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine)
mutable notes: Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Trojan peptide
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792004_sequence/1

Trojan peptide
type: SequenceAnnotation
persistent identity: annotation2197880
display id: annotation2197880
version: 1
top level: Polyargini
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792004/annotation2197880/range2197880/1

https://synbiohub.org/public/igem/BBa_K792004/annotation2197880/range2197880/1
type: Range
persistent identity: range2197880
display id: range2197880
version: 1
direction: direction/forward
top level: Polyargini
start: 7
end: 39
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196615
display id: annotation2196615
version: 1
top level: Polyargini
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792004/annotation2196615/range2196615/1

https://synbiohub.org/public/igem/BBa_K792004/annotation2196615/range2196615/1
type: Range
persistent identity: range2196615
display id: range2196615
version: 1
direction: direction/forward
top level: Polyargini
start: 1
end: 6
orientation: inline

BBa_K950009
type: ComponentDefinition
persistent identity: BBa K950009
display id: BBa_K950009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast mig1
creator: Jakob Matthes
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mig1 is a repressor targeting the promotor of suc2.
mutable notes: original sequence was not altered
mutable provenance: Sequence and information is based on the Saccharomyces Genome Database, see http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=mig1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mig1
sequence: https://synbiohub.org/public/igem/BBa_K950009_sequence/1

mig1
type: SequenceAnnotation
persistent identity: annotation2182740
display id: annotation2182740
version: 1
top level: BBa_K950009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950009/annotation2182740/range2182740/1

https://synbiohub.org/public/igem/BBa_K950009/annotation2182740/range2182740/1
type: Range
persistent identity: range2182740
display id: range2182740
version: 1
direction: direction/forward
top level: BBa_K950009
start: 1
end: 1515
orientation: inline

BBa_K431007
type: ComponentDefinition
persistent identity: BBa K431007
display id: BBa_K431007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alcohol Oxidase 1 promoter
creator: David Joseph Sexton Jr.
created: 2010-09-16T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _570_
m user id: 0
owner id: 6820
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pichia pastoris initially gained attention as an expression system after recognition of its methylotrophic capabilities and the high levels of alcohol oxidase(AOX) present when induced by methanol. By isolating the promoter (pAOX1 )responsible for AOX production, this system can be utilized to obtain high yields of heterologous protein. The promoter provides not only high expression but very tight regulation as well. Vectors providing protein expression under the control of pAOX1 are the primary system used for P. pastoris
mutable notes: This part was obtained by designing primers and isolating it from the organisms DNA. There were no unwanted restriction enzyme sites to eliminate
mutable provenance: Isolated from Pichia pastoris
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_K431007_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2080187
display id: annotation2080187
version: 1
top level: BBa_K431007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K431007/annotation2080187/range2080187/1

https://synbiohub.org/public/igem/BBa_K431007/annotation2080187/range2080187/1
type: Range
persistent identity: range2080187
display id: range2080187
version: 1
direction: direction/forward
top level: BBa_K431007
start: 1
end: 940
orientation: inline

BBa_K1861040
type: ComponentDefinition
persistent identity: BBa K1861040
display id: BBa_K1861040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SpyCatcher and RPD3 in an expression cassette
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:21:28Z
discontinued: false
dominant: false
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1159200
component: BBa_K392003
component: BBa_K1861401
component: BBa_K1861102
component: BBa_K1861101
component: BBa_K1861104
sequence annotation: BBa_K1861401
sequence annotation: BBa_K1861102
sequence annotation: BBa_K1861101
sequence annotation: BBa_K1861104
sequence annotation: BBa_K392003
sequence annotation: BBa_K1159200
sequence: https://synbiohub.org/public/igem/BBa_K1861040_sequence/1

BBa_K1159200
type: Component
persistent identity: component2474406
display id: component2474406
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1159200/1

BBa_K392003
type: Component
persistent identity: component2474409
display id: component2474409
version: 1
top level: BBa_K1861040
access: public
definition: BBa_K392003

BBa_K1861401
type: Component
persistent identity: component2474407
display id: component2474407
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861401/1

BBa_K1861102
type: Component
persistent identity: component2474401
display id: component2474401
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861102/1

BBa_K1861101
type: Component
persistent identity: component2474400
display id: component2474400
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861101/1

BBa_K1861104
type: Component
persistent identity: component2474408
display id: component2474408
version: 1
top level: BBa_K1861040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861104/1

BBa_K1861401
type: SequenceAnnotation
persistent identity: annotation2474407
display id: annotation2474407
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
component: BBa_K1861401

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474407/range2474407/1
type: Range
persistent identity: range2474407
display id: range2474407
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 605
end: 1954
orientation: inline

BBa_K1861102
type: SequenceAnnotation
persistent identity: annotation2474401
display id: annotation2474401
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
component: BBa_K1861102

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474401/range2474401/1
type: Range
persistent identity: range2474401
display id: range2474401
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 239
end: 265
orientation: inline

BBa_K1861101
type: SequenceAnnotation
persistent identity: annotation2474400
display id: annotation2474400
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
component: BBa_K1861101

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474400/range2474400/1
type: Range
persistent identity: range2474400
display id: range2474400
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1
end: 238
orientation: inline

BBa_K1861104
type: SequenceAnnotation
persistent identity: annotation2474408
display id: annotation2474408
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
component: BBa_K1861104

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474408/range2474408/1
type: Range
persistent identity: range2474408
display id: range2474408
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1955
end: 1963
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2474409
display id: annotation2474409
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474409/range2474409/1
type: Range
persistent identity: range2474409
display id: range2474409
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 1964
end: 2092
orientation: inline

BBa_K1159200
type: SequenceAnnotation
persistent identity: annotation2474406
display id: annotation2474406
version: 1
top level: BBa_K1861040
location: https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
component: BBa_K1159200

https://synbiohub.org/public/igem/BBa_K1861040/annotation2474406/range2474406/1
type: Range
persistent identity: range2474406
display id: range2474406
version: 1
direction: direction/reverse
top level: BBa_K1861040
start: 266
end: 604
orientation: inline

BBa_K165053
type: ComponentDefinition
persistent identity: BBa K165053
display id: BBa_K165053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full LexA Repressor (tagged with CFPx2)
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Kozak+Sin3p RD+LexA DBD+CFPx2+SV40NLS+tADH1
mutable notes: -
mutable provenance: Constructed in our lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K165018
component: BBa_K165014
component: BBa_K165002
component: BBa_K165009
component: BBa_K165003
sequence annotation: BBa_K165003
sequence annotation: BBa_K165009
sequence annotation: BBa_K165002
sequence annotation: BBa_K165018
sequence annotation: BBa_K165014
sequence: https://synbiohub.org/public/igem/BBa_K165053_sequence/1

BBa_K165018
type: Component
persistent identity: component1996734
display id: component1996734
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165018/1

BBa_K165014
type: Component
persistent identity: component1996733
display id: component1996733
version: 1
top level: BBa_K165053
access: public
definition: cfpx2

BBa_K165002
type: Component
persistent identity: component1996730
display id: component1996730
version: 1
top level: BBa_K165053
access: public
definition: BBa_K165002

BBa_K165009
type: Component
persistent identity: component1996731
display id: component1996731
version: 1
top level: BBa_K165053
access: public
definition: https://synbiohub.org/public/igem/BBa_K165009/1

BBa_K165003
type: Component
persistent identity: component1996732
display id: component1996732
version: 1
top level: BBa_K165053
access: public
definition: Sin3

BBa_K165003
type: SequenceAnnotation
persistent identity: annotation1996732
display id: annotation1996732
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996732/range1996732/1
component: BBa_K165003

https://synbiohub.org/public/igem/BBa_K165053/annotation1996732/range1996732/1
type: Range
persistent identity: range1996732
display id: range1996732
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 293
end: 3103
orientation: inline

BBa_K165009
type: SequenceAnnotation
persistent identity: annotation1996731
display id: annotation1996731
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996731/range1996731/1
component: BBa_K165009

https://synbiohub.org/public/igem/BBa_K165053/annotation1996731/range1996731/1
type: Range
persistent identity: range1996731
display id: range1996731
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 27
end: 284
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1996730
display id: annotation1996730
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996730/range1996730/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165053/annotation1996730/range1996730/1
type: Range
persistent identity: range1996730
display id: range1996730
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 1
end: 18
orientation: inline

BBa_K165018
type: SequenceAnnotation
persistent identity: annotation1996734
display id: annotation1996734
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996734/range1996734/1
component: BBa_K165018

https://synbiohub.org/public/igem/BBa_K165053/annotation1996734/range1996734/1
type: Range
persistent identity: range1996734
display id: range1996734
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 4614
end: 4866
orientation: inline

BBa_K165014
type: SequenceAnnotation
persistent identity: annotation1996733
display id: annotation1996733
version: 1
top level: BBa_K165053
location: https://synbiohub.org/public/igem/BBa_K165053/annotation1996733/range1996733/1
component: BBa_K165014

https://synbiohub.org/public/igem/BBa_K165053/annotation1996733/range1996733/1
type: Range
persistent identity: range1996733
display id: range1996733
version: 1
direction: direction/reverse
top level: BBa_K165053
start: 3112
end: 4605
orientation: inline

BBa_K1680021
type: ComponentDefinition
persistent identity: BBa K1680021
display id: BBa_K1680021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T01:27:03Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: SV40 NLS
sequence annotation: Dronpa
sequence annotation: Cre recombinase
sequence: https://synbiohub.org/public/igem/BBa_K1680021_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2477375
display id: annotation2477375
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1
type: Range
persistent identity: range2477375
display id: range2477375
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 700
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477373
display id: annotation2477373
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1
type: Range
persistent identity: range2477373
display id: range2477373
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477374
display id: annotation2477374
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1
type: Range
persistent identity: range2477374
display id: range2477374
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 31
end: 699
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477376
display id: annotation2477376
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1
type: Range
persistent identity: range2477376
display id: range2477376
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 718
end: 1746
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477377
display id: annotation2477377
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1
type: Range
persistent identity: range2477377
display id: range2477377
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1747
end: 1764
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477378
display id: annotation2477378
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1
type: Range
persistent identity: range2477378
display id: range2477378
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1765
end: 2433
orientation: inline

BBa_K165057
type: ComponentDefinition
persistent identity: BBa K165057
display id: BBa_K165057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak + mCherry
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K165004
component: BBa_K165002
sequence annotation: BBa_K165004
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K165057_sequence/1

BBa_K165004
type: Component
persistent identity: component1997047
display id: component1997047
version: 1
top level: BBa_K165057
access: public
definition: mCherry

BBa_K165002
type: Component
persistent identity: component1997046
display id: component1997046
version: 1
top level: BBa_K165057
access: public
definition: BBa_K165002

BBa_K165004
type: SequenceAnnotation
persistent identity: annotation1997047
display id: annotation1997047
version: 1
top level: BBa_K165057
location: https://synbiohub.org/public/igem/BBa_K165057/annotation1997047/range1997047/1
component: BBa_K165004

https://synbiohub.org/public/igem/BBa_K165057/annotation1997047/range1997047/1
type: Range
persistent identity: range1997047
display id: range1997047
version: 1
direction: direction/reverse
top level: BBa_K165057
start: 27
end: 761
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation1997046
display id: annotation1997046
version: 1
top level: BBa_K165057
location: https://synbiohub.org/public/igem/BBa_K165057/annotation1997046/range1997046/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K165057/annotation1997046/range1997046/1
type: Range
persistent identity: range1997046
display id: range1997046
version: 1
direction: direction/reverse
top level: BBa_K165057
start: 1
end: 18
orientation: inline

BBa_K284002
type: ComponentDefinition
persistent identity: BBa K284002
display id: BBa_K284002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: JEN1 Promoter from Kluyveromyces lactis
creator: Raissa Estrela Curado, Tais Sineiro Herig and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: TATA-Like
sequence annotation: JEN1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K284002_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034979
display id: annotation2034979
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1
type: Range
persistent identity: range2034979
display id: range2034979
version: 1
direction: direction/reverse
top level: BBa_K284002
start: 976
end: 998
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034977
display id: annotation2034977
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1
type: Range
persistent identity: range2034977
display id: range2034977
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 19
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2040986
display id: annotation2040986
version: 1
top level: BBa_K284002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1
type: Range
persistent identity: range2040986
display id: range2040986
version: 1
direction: direction/forward
top level: BBa_K284002
start: 862
end: 873
orientation: inline

JEN1 Promoter
type: SequenceAnnotation
persistent identity: annotation2034978
display id: annotation2034978
version: 1
top level: BBa_K284002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1
type: Range
persistent identity: range2034978
display id: range2034978
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 998
orientation: inline

BBa_K1907005
type: ComponentDefinition
persistent identity: BBa K1907005
display id: BBa_K1907005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCP1 promoter for S. cerevisiae
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T12:28:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1907005_sequence/1

BBa_K1486032
type: ComponentDefinition
persistent identity: BBa K1486032
display id: BBa_K1486032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + ADH1 terminator + Ura3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 8 a.a linker
sequence annotation: Hog1 5' flanking region
sequence annotation: BBa_K1486023
sequence annotation: BBa_K1486031
sequence annotation: ADH1 terminator
sequence annotation: Hog1 3' flanking region
sequence: https://synbiohub.org/public/igem/BBa_K1486032_sequence/1

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417800
display id: annotation2417800
version: 1
top level: BBa_K1486032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1
type: Range
persistent identity: range2417800
display id: range2417800
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 48
end: 71
orientation: inline

Hog1 5' flanking region
type: SequenceAnnotation
persistent identity: annotation2417799
display id: annotation2417799
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1
type: Range
persistent identity: range2417799
display id: range2417799
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1
end: 47
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417801
display id: annotation2417801
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1
type: Range
persistent identity: range2417801
display id: range2417801
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 72
end: 804
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2417804
display id: annotation2417804
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1
type: Range
persistent identity: range2417804
display id: range2417804
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1070
end: 2430
orientation: inline

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation2417803
display id: annotation2417803
version: 1
top level: BBa_K1486032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1
type: Range
persistent identity: range2417803
display id: range2417803
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 808
end: 996
orientation: inline

Hog1 3' flanking region
type: SequenceAnnotation
persistent identity: annotation2417805
display id: annotation2417805
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1
type: Range
persistent identity: range2417805
display id: range2417805
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 2494
end: 2544
orientation: inline