//chassis/eukaryote/plants/other
type: Collection
persistent identity: chassis eukaryote plants other
display id: chassis_eukaryote_plants_other
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1751002
member: BBa_K2017003
member: BBa_K2017004
member: BBa_K2017005
member: BBa_K2017006

//chassis/eukaryote/plants
type: Collection
persistent identity: chassis eukaryote plants
display id: chassis_eukaryote_plants
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //chassis/eukaryote/plants/other

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: polya
sequence annotation: stop
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_K2017003
type: ComponentDefinition
persistent identity: BBa K2017003
display id: BBa_K2017003
version: 1
was generated by: iGEM to SBOL conversion
description: SAGTI linker + Luciferase
created: 2016-10-02T11:00:00Z
modified: 2016-10-23T08:34:14Z
mutable provenance: Luciferase was obtained from laboratory stock. The linker was fused with a PCR.
owned by: james
owned by: myers
mutable description: Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused to the 3' with other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to 3' of other coding sequence. The translation must begin in the coding sequence fused to the 3'. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part must be fused to the 3' of other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused to the 3'. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
star: false
creator: Monica Victoria Gutierrez Salazar
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Additional nucleotide
sequence annotation: Luciferase
sequence annotation: SAGTI Linker
sequence: https://synbiohub.org/public/igem/BBa_K2017003_sequence/1

Additional nucleotide
type: SequenceAnnotation
persistent identity: annotation2486025
display id: annotation2486025
version: 1
top level: BBa_K2017003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017003/annotation2486025/range2486025/1

https://synbiohub.org/public/igem/BBa_K2017003/annotation2486025/range2486025/1
type: Range
persistent identity: range2486025
display id: range2486025
version: 1
top level: BBa_K2017003
direction: direction/forward
start: 1
end: 1
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2486023
display id: annotation2486023
version: 1
top level: BBa_K2017003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2017003/annotation2486023/range2486023/1

https://synbiohub.org/public/igem/BBa_K2017003/annotation2486023/range2486023/1
type: Range
persistent identity: range2486023
display id: range2486023
version: 1
top level: BBa_K2017003
direction: direction/forward
start: 16
end: 1667
orientation: inline

SAGTI Linker
type: SequenceAnnotation
persistent identity: annotation2486024
display id: annotation2486024
version: 1
top level: BBa_K2017003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017003/annotation2486024/range2486024/1

https://synbiohub.org/public/igem/BBa_K2017003/annotation2486024/range2486024/1
type: Range
persistent identity: range2486024
display id: range2486024
version: 1
top level: BBa_K2017003
direction: direction/forward
start: 2
end: 15
orientation: inline

BBa_K2017005
type: ComponentDefinition
persistent identity: BBa K2017005
display id: BBa_K2017005
version: 1
was generated by: iGEM to SBOL conversion
description: AEK linker + Luciferase
created: 2016-10-02T11:00:00Z
modified: 2016-10-23T08:33:52Z
mutable provenance: Luciferase was obtained from laboratory stock. The linker was fused with a PCR.
owned by: james
owned by: myers
mutable description: Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the AEK linker [A(EAAAK)3A] in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: This part must be fused downstream to other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
star: false
creator: Monica Victoria Gutierrez Salazar
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Additional nucleotides
sequence annotation: AEK linker
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K2017005_sequence/1

Additional nucleotides
type: SequenceAnnotation
persistent identity: annotation2486697
display id: annotation2486697
version: 1
top level: BBa_K2017005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017005/annotation2486697/range2486697/1

https://synbiohub.org/public/igem/BBa_K2017005/annotation2486697/range2486697/1
type: Range
persistent identity: range2486697
display id: range2486697
version: 1
top level: BBa_K2017005
direction: direction/forward
start: 1
end: 2
orientation: inline

AEK linker
type: SequenceAnnotation
persistent identity: annotation2486698
display id: annotation2486698
version: 1
top level: BBa_K2017005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017005/annotation2486698/range2486698/1

https://synbiohub.org/public/igem/BBa_K2017005/annotation2486698/range2486698/1
type: Range
persistent identity: range2486698
display id: range2486698
version: 1
top level: BBa_K2017005
direction: direction/forward
start: 3
end: 50
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2486700
display id: annotation2486700
version: 1
top level: BBa_K2017005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2017005/annotation2486700/range2486700/1

https://synbiohub.org/public/igem/BBa_K2017005/annotation2486700/range2486700/1
type: Range
persistent identity: range2486700
display id: range2486700
version: 1
top level: BBa_K2017005
direction: direction/forward
start: 51
end: 1703
orientation: inline

BBa_K2017006
type: ComponentDefinition
persistent identity: BBa K2017006
display id: BBa_K2017006
version: 1
was generated by: iGEM to SBOL conversion
description: RSIAT-TEV linker + Luciferase
created: 2016-10-02T11:00:00Z
modified: 2016-10-22T07:17:21Z
mutable provenance: Luciferase was obtained from laboratory stock. The linker was fused with a PCR.
owned by: james
owned by: myers
mutable description: Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the RSIAT-TEV linker in order to let the luciferase acquire the correct tertiary structure. The TEV region is the recognition site for the NiaTEV protease. The protease cuts on its site, letting the luciferase without the upstream coding sequence. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part must be fused downstream to other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
star: false
creator: Monica Victoria Gutierrez Salazar
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Additional nucleotide
sequence annotation: Luciferase
sequence annotation: RSIAT Linker
sequence: https://synbiohub.org/public/igem/BBa_K2017006_sequence/1

Additional nucleotide
type: SequenceAnnotation
persistent identity: annotation2487377
display id: annotation2487377
version: 1
top level: BBa_K2017006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017006/annotation2487377/range2487377/1

https://synbiohub.org/public/igem/BBa_K2017006/annotation2487377/range2487377/1
type: Range
persistent identity: range2487377
display id: range2487377
version: 1
top level: BBa_K2017006
direction: direction/forward
start: 1
end: 2
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2487382
display id: annotation2487382
version: 1
top level: BBa_K2017006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2017006/annotation2487382/range2487382/1

https://synbiohub.org/public/igem/BBa_K2017006/annotation2487382/range2487382/1
type: Range
persistent identity: range2487382
display id: range2487382
version: 1
top level: BBa_K2017006
direction: direction/forward
start: 20
end: 1700
orientation: inline

RSIAT Linker
type: SequenceAnnotation
persistent identity: annotation2487378
display id: annotation2487378
version: 1
top level: BBa_K2017006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017006/annotation2487378/range2487378/1

https://synbiohub.org/public/igem/BBa_K2017006/annotation2487378/range2487378/1
type: Range
persistent identity: range2487378
display id: range2487378
version: 1
top level: BBa_K2017006
direction: direction/forward
start: 3
end: 19
orientation: inline

BBa_K1751000
type: ComponentDefinition
persistent identity: BBa K1751000
display id: BBa_K1751000
version: 1
was generated by: iGEM to SBOL conversion
description: RgPAL1：phenylalanine ammonia lyase (PAL) coding region
created: 2015-08-02T11:00:00Z
modified: 2015-08-19T04:47:14Z
mutable provenance: Rehmannia glutinosa.
owned by: james
owned by: myers
mutable description: The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an??enzyme??that??catalyzes??a reaction converting L-phenylalanine??to ammonia??and??trans-cinnamic acid.PAL is the first and committed step in the??phenyl propanoid pathway??and is therefore involved in the??biosynthesis??of the??polyphenol??compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2173_
m user id: 28395
owner id: 28395
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）.
star: false
creator: Tianlai Xia
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PAL coding region
sequence: https://synbiohub.org/public/igem/BBa_K1751000_sequence/1

PAL coding region
type: SequenceAnnotation
persistent identity: annotation2434421
display id: annotation2434421
version: 1
top level: BBa_K1751000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1751000/annotation2434421/range2434421/1

https://synbiohub.org/public/igem/BBa_K1751000/annotation2434421/range2434421/1
type: Range
persistent identity: range2434421
display id: range2434421
version: 1
top level: BBa_K1751000
direction: direction/forward
start: 1
end: 2127
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K2017004
type: ComponentDefinition
persistent identity: BBa K2017004
display id: BBa_K2017004
version: 1
was generated by: iGEM to SBOL conversion
description: RSIAT linker + Luciferase
created: 2016-10-02T11:00:00Z
modified: 2016-10-23T08:34:29Z
mutable provenance: Luciferase was obtained from laboratory stock. The linker was fused with a PCR.
owned by: james
owned by: myers
mutable description: Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the RSIAT linker in order to let the luciferase acquire the correct tertiary structure. The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part must be fused downstream to other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.
star: false
creator: Monica Victoria Gutierrez Salazar
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RSIAT Linker
sequence annotation: Additional nucleotides
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K2017004_sequence/1

RSIAT Linker
type: SequenceAnnotation
persistent identity: annotation2486027
display id: annotation2486027
version: 1
top level: BBa_K2017004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017004/annotation2486027/range2486027/1

https://synbiohub.org/public/igem/BBa_K2017004/annotation2486027/range2486027/1
type: Range
persistent identity: range2486027
display id: range2486027
version: 1
top level: BBa_K2017004
direction: direction/forward
start: 3
end: 16
orientation: inline

Additional nucleotides
type: SequenceAnnotation
persistent identity: annotation2486028
display id: annotation2486028
version: 1
top level: BBa_K2017004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017004/annotation2486028/range2486028/1

https://synbiohub.org/public/igem/BBa_K2017004/annotation2486028/range2486028/1
type: Range
persistent identity: range2486028
display id: range2486028
version: 1
top level: BBa_K2017004
direction: direction/forward
start: 1
end: 2
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2486026
display id: annotation2486026
version: 1
top level: BBa_K2017004
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2017004/annotation2486026/range2486026/1

https://synbiohub.org/public/igem/BBa_K2017004/annotation2486026/range2486026/1
type: Range
persistent identity: range2486026
display id: range2486026
version: 1
top level: BBa_K2017004
direction: direction/forward
start: 17
end: 1667
orientation: inline

BBa_K1751002
type: ComponentDefinition
persistent identity: BBa K1751002
display id: BBa_K1751002
version: 1
was generated by: iGEM to SBOL conversion
description: Promotor-RBS+RgPAL1+Terminator
created: 2015-08-15T11:00:00Z
modified: 2015-12-16T01:48:14Z
mutable provenance: iGEM's database and Rehmannia glutinosa Genome
owned by: james
owned by: myers
mutable description: Promotor-RBS:This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119. RgPAL1:The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an enzyme that catalyzes a reaction converting L-phenylalanine to ammonia and trans-cinnamic acid.PAL is the first and committed step in the phenyl propanoid pathway and is therefore involved in the biosynthesis of the polyphenol compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
discontinued: false
dominant: false
experience: experience/None
group u list: _2173_
m user id: 4206
owner id: 28395
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Promotor-RBS+RgPAL1
star: false
creator: Tianlai Xia
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_K1751000
component: BBa_B0010
component: BBa_K847100
sequence annotation: BBa_K847100
sequence annotation: BBa_B0010
sequence annotation: BBa_K1751000
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1751002_sequence/1

BBa_B0012
type: Component
persistent identity: component2436450
display id: component2436450
version: 1
top level: BBa_K1751002
access: public
definition: BBa_B0012

BBa_K1751000
type: Component
persistent identity: component2436447
display id: component2436447
version: 1
top level: BBa_K1751002
access: public
definition: BBa_K1751000

BBa_B0010
type: Component
persistent identity: component2436448
display id: component2436448
version: 1
top level: BBa_K1751002
access: public
definition: BBa_B0010

BBa_K847100
type: Component
persistent identity: component2436445
display id: component2436445
version: 1
top level: BBa_K1751002
access: public
definition: BBa_K847100

BBa_K847100
type: SequenceAnnotation
persistent identity: annotation2436445
display id: annotation2436445
version: 1
top level: BBa_K1751002
location: https://synbiohub.org/public/igem/BBa_K1751002/annotation2436445/range2436445/1
component: BBa_K847100

https://synbiohub.org/public/igem/BBa_K1751002/annotation2436445/range2436445/1
type: Range
persistent identity: range2436445
display id: range2436445
version: 1
top level: BBa_K1751002
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2436448
display id: annotation2436448
version: 1
top level: BBa_K1751002
location: https://synbiohub.org/public/igem/BBa_K1751002/annotation2436448/range2436448/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1751002/annotation2436448/range2436448/1
type: Range
persistent identity: range2436448
display id: range2436448
version: 1
top level: BBa_K1751002
direction: direction/forward
start: 2200
end: 2279
orientation: inline

BBa_K1751000
type: SequenceAnnotation
persistent identity: annotation2436447
display id: annotation2436447
version: 1
top level: BBa_K1751002
location: https://synbiohub.org/public/igem/BBa_K1751002/annotation2436447/range2436447/1
component: BBa_K1751000

https://synbiohub.org/public/igem/BBa_K1751002/annotation2436447/range2436447/1
type: Range
persistent identity: range2436447
display id: range2436447
version: 1
top level: BBa_K1751002
direction: direction/reverse
start: 65
end: 2191
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2436450
display id: annotation2436450
version: 1
top level: BBa_K1751002
location: https://synbiohub.org/public/igem/BBa_K1751002/annotation2436450/range2436450/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1751002/annotation2436450/range2436450/1
type: Range
persistent identity: range2436450
display id: range2436450
version: 1
top level: BBa_K1751002
direction: direction/forward
start: 2288
end: 2328
orientation: inline

BBa_K847100
type: ComponentDefinition
persistent identity: BBa K847100
display id: BBa_K847100
version: 1
was generated by: iGEM to SBOL conversion
description: Promotor-RBS
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:34Z
mutable provenance: http://partsregistry.org/Part:BBa_B0030 http://partsregistry.org/Part:BBa_J23100
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119.
discontinued: false
dominant: false
experience: experience/None
group u list: _1107_
m user id: 0
owner id: 12286
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Bella A Okiddy, Julia Borden, Michelle Yu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K847100_sequence/1

BBa_B0030
type: Component
persistent identity: component2210121
display id: component2210121
version: 1
top level: BBa_K847100
access: public
definition: BBa_B0030

BBa_J23100
type: Component
persistent identity: component2210119
display id: component2210119
version: 1
top level: BBa_K847100
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2210119
display id: annotation2210119
version: 1
top level: BBa_K847100
location: https://synbiohub.org/public/igem/BBa_K847100/annotation2210119/range2210119/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K847100/annotation2210119/range2210119/1
type: Range
persistent identity: range2210119
display id: range2210119
version: 1
top level: BBa_K847100
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2210121
display id: annotation2210121
version: 1
top level: BBa_K847100
location: https://synbiohub.org/public/igem/BBa_K847100/annotation2210121/range2210121/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K847100/annotation2210121/range2210121/1
type: Range
persistent identity: range2210121
display id: range2210121
version: 1
top level: BBa_K847100
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2017005_sequence/1
type: Sequence
persistent identity: BBa K2017005 sequence
display id: BBa_K2017005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggctgaagctgctgctaaggaagctgctgctaaggaagctgctgctaaggctgaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1751000_sequence/1
type: Sequence
persistent identity: BBa K1751000 sequence
display id: BBa_K1751000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacggaatgcagatcgtatccgttgtacaagtttatcagagaggaacttgggacgaactacttgacggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2017004_sequence/1
type: Sequence
persistent identity: BBa K2017004 sequence
display id: BBa_K2017004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgagatctattgctactgaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1751002_sequence/1
type: Sequence
persistent identity: BBa K1751002 sequence
display id: BBa_K1751002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2017006_sequence/1
type: Sequence
persistent identity: BBa K2017006 sequence
display id: BBa_K2017006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgagatctattgctactactactgagaacctctactttcagagtggcactgaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K847100_sequence/1
type: Sequence
persistent identity: BBa K847100 sequence
display id: BBa_K847100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2017003_sequence/1
type: Sequence
persistent identity: BBa K2017003 sequence
display id: BBa_K2017003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagcgcaggaaccattggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z