//chassis/eukaryote/nbenthamiana
type: Collection
persistent identity: chassis eukaryote nbenthamiana
display id: chassis_eukaryote_nbenthamiana
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1618033
member: BBa_K2017007
member: BBa_K1742010
member: BBa_K1554000
member: BBa_K1554004
member: BBa_K1742006
member: BBa_K1467101
member: BBa_K1742001
member: BBa_K2017010
member: BBa_K1618034
member: BBa_K2017008
member: BBa_K2017014
member: BBa_K1467204
member: BBa_K1618032
member: BBa_K1554003
member: BBa_K1742005
member: BBa_K1742008
member: BBa_K1618028
member: BBa_K2017001
member: BBa_K1618029
member: BBa_K1618037
member: BBa_K2017009
member: AsLOV2pep
member: BBa_K1742013
member: BBa_K1467104
member: BBa_K1467203
member: BBa_K1742004
member: BBa_K1742009
member: BBa_K2017013
member: BBa_K1554002
member: BBa_K2017000
member: BBa_K1618030
member: BBa_K2017012
member: BBa_K1742012
member: BBa_K1467103
member: BBa_K1618036
member: BBa_K1742011
member: BBa_K1742003
member: BBa_K1554001
member: BBa_K1554005
member: BBa_K1467205
member: BBa_K2017011
member: BBa_K1618031
member: BBa_K1742002
member: BBa_K1467102
member: BBa_K1618035

BBa_K2017008
type: ComponentDefinition
persistent identity: BBa K2017008
display id: BBa_K2017008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + Ga20ox consense + RSIAT-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T10:32:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s with a 5' region (BBa_K2017002), 20 nucleotides of the Ga20ox consensus gene of Oryza sativa (rice), the reporter luciferase with RSIAT linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the Ga20ox fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'
mutable provenance: This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537015
component: BBa_K2017004
component: BBa_K1484215
sequence annotation: BBa_K1484215
sequence annotation: BBa_K1537015
sequence annotation: BBa_K2017004
sequence: https://synbiohub.org/public/igem/BBa_K2017008_sequence/1

BBa_K1537015
type: Component
persistent identity: component2487404
display id: component2487404
version: 1
top level: BBa_K2017008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K2017004
type: Component
persistent identity: component2487408
display id: component2487408
version: 1
top level: BBa_K2017008
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017004/1

BBa_K1484215
type: Component
persistent identity: component2487411
display id: component2487411
version: 1
top level: BBa_K2017008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487411
display id: annotation2487411
version: 1
top level: BBa_K2017008
location: https://synbiohub.org/public/igem/BBa_K2017008/annotation2487411/range2487411/1
component: BBa_K1484215

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type: Range
persistent identity: range2487411
display id: range2487411
version: 1
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end: 2836
orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487404
display id: annotation2487404
version: 1
top level: BBa_K2017008
location: https://synbiohub.org/public/igem/BBa_K2017008/annotation2487404/range2487404/1
component: BBa_K1537015

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type: Range
persistent identity: range2487404
display id: range2487404
version: 1
direction: direction/reverse
top level: BBa_K2017008
start: 1
end: 860
orientation: inline

BBa_K2017004
type: SequenceAnnotation
persistent identity: annotation2487408
display id: annotation2487408
version: 1
top level: BBa_K2017008
location: https://synbiohub.org/public/igem/BBa_K2017008/annotation2487408/range2487408/1
component: BBa_K2017004

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type: Range
persistent identity: range2487408
display id: range2487408
version: 1
direction: direction/reverse
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BBa_K1467104
type: ComponentDefinition
persistent identity: BBa K1467104
display id: BBa_K1467104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible MAS promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:22:30Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MAS is a plant specific promoter which is constitutively expressed. This part is compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
mutable notes: Domestication for GoldenGate by removal of BsaI and BpiI recognition sequences.
mutable provenance: Agrobacterium tumefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467104_sequence/1

BBa_K1618030
type: ComponentDefinition
persistent identity: BBa K1618030
display id: BBa_K1618030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 2 -Fused to GFP -Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:56:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates starch with acetyltransferase RV3030 with a GFP tag for visualization of localisation.
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618002
component: BBa_K1618037
component: BBa_K1467204
component: BBa_K1618028
component: BBa_K1467101
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1618002
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1467204
sequence annotation: BBa_K1618028
sequence: https://synbiohub.org/public/igem/BBa_K1618030_sequence/1

BBa_K1618002
type: Component
persistent identity: component2462175
display id: component2462175
version: 1
top level: BBa_K1618030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618002/1

BBa_K1618037
type: Component
persistent identity: component2462177
display id: component2462177
version: 1
top level: BBa_K1618030
access: public
definition: BBa_K1618037

BBa_K1467204
type: Component
persistent identity: component2462176
display id: component2462176
version: 1
top level: BBa_K1618030
access: public
definition: BBa_K1467204

BBa_K1618028
type: Component
persistent identity: component2462174
display id: component2462174
version: 1
top level: BBa_K1618030
access: public
definition: BBa_K1618028

BBa_K1467101
type: Component
persistent identity: component2462173
display id: component2462173
version: 1
top level: BBa_K1618030
access: public
definition: BBa_K1467101

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462177
display id: annotation2462177
version: 1
top level: BBa_K1618030
location: https://synbiohub.org/public/igem/BBa_K1618030/annotation2462177/range2462177/1
component: BBa_K1618037

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display id: range2462177
version: 1
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orientation: inline

BBa_K1618002
type: SequenceAnnotation
persistent identity: annotation2462175
display id: annotation2462175
version: 1
top level: BBa_K1618030
location: https://synbiohub.org/public/igem/BBa_K1618030/annotation2462175/range2462175/1
component: BBa_K1618002

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type: Range
persistent identity: range2462175
display id: range2462175
version: 1
direction: direction/reverse
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end: 2449
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BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462173
display id: annotation2462173
version: 1
top level: BBa_K1618030
location: https://synbiohub.org/public/igem/BBa_K1618030/annotation2462173/range2462173/1
component: BBa_K1467101

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type: Range
persistent identity: range2462173
display id: range2462173
version: 1
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start: 1
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BBa_K1467204
type: SequenceAnnotation
persistent identity: annotation2462176
display id: annotation2462176
version: 1
top level: BBa_K1618030
location: https://synbiohub.org/public/igem/BBa_K1618030/annotation2462176/range2462176/1
component: BBa_K1467204

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type: Range
persistent identity: range2462176
display id: range2462176
version: 1
direction: direction/reverse
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BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462174
display id: annotation2462174
version: 1
top level: BBa_K1618030
location: https://synbiohub.org/public/igem/BBa_K1618030/annotation2462174/range2462174/1
component: BBa_K1618028

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type: Range
persistent identity: range2462174
display id: range2462174
version: 1
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BBa_K2017007
type: ComponentDefinition
persistent identity: BBa K2017007
display id: BBa_K2017007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s:5'+ Ga20ox consense + SAGTI-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-02T11:00:00Z
modified: 2016-10-22T04:50:07Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3') It includes a promoter 35s with a 5' region (BBa_K2017002), 20 nucleotides of the Ga20ox consensus gene of Oryza sativa (rice), the reporter luciferase with SAGTI linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the Ga20ox fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'
mutable provenance: This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1484215
component: BBa_K2017002
component: BBa_K2017003
sequence annotation: BBa_K2017002
sequence annotation: BBa_K2017003
sequence annotation: BBa_K1484215
sequence: https://synbiohub.org/public/igem/BBa_K2017007_sequence/1

BBa_K1484215
type: Component
persistent identity: component2487400
display id: component2487400
version: 1
top level: BBa_K2017007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K2017002
type: Component
persistent identity: component2487394
display id: component2487394
version: 1
top level: BBa_K2017007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017002/1

BBa_K2017003
type: Component
persistent identity: component2487397
display id: component2487397
version: 1
top level: BBa_K2017007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017003/1

BBa_K2017002
type: SequenceAnnotation
persistent identity: annotation2487394
display id: annotation2487394
version: 1
top level: BBa_K2017007
location: https://synbiohub.org/public/igem/BBa_K2017007/annotation2487394/range2487394/1
component: BBa_K2017002

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type: Range
persistent identity: range2487394
display id: range2487394
version: 1
direction: direction/reverse
top level: BBa_K2017007
start: 1
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orientation: inline

BBa_K2017003
type: SequenceAnnotation
persistent identity: annotation2487397
display id: annotation2487397
version: 1
top level: BBa_K2017007
location: https://synbiohub.org/public/igem/BBa_K2017007/annotation2487397/range2487397/1
component: BBa_K2017003

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type: Range
persistent identity: range2487397
display id: range2487397
version: 1
direction: direction/reverse
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start: 1090
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BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487400
display id: annotation2487400
version: 1
top level: BBa_K2017007
location: https://synbiohub.org/public/igem/BBa_K2017007/annotation2487400/range2487400/1
component: BBa_K1484215

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type: Range
persistent identity: range2487400
display id: range2487400
version: 1
direction: direction/reverse
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start: 2765
end: 3057
orientation: inline

BBa_K1618036
type: ComponentDefinition
persistent identity: BBa K1618036
display id: BBa_K1618036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 4 Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:58:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates Starch
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618004
component: BBa_K1618028
component: BBa_K1467101
component: BBa_K1618037
sequence annotation: BBa_K1618004
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618028
sequence: https://synbiohub.org/public/igem/BBa_K1618036_sequence/1

BBa_K1618004
type: Component
persistent identity: component2462166
display id: component2462166
version: 1
top level: BBa_K1618036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618004/1

BBa_K1618028
type: Component
persistent identity: component2462165
display id: component2462165
version: 1
top level: BBa_K1618036
access: public
definition: BBa_K1618028

BBa_K1467101
type: Component
persistent identity: component2462164
display id: component2462164
version: 1
top level: BBa_K1618036
access: public
definition: BBa_K1467101

BBa_K1618037
type: Component
persistent identity: component2462167
display id: component2462167
version: 1
top level: BBa_K1618036
access: public
definition: BBa_K1618037

BBa_K1618004
type: SequenceAnnotation
persistent identity: annotation2462166
display id: annotation2462166
version: 1
top level: BBa_K1618036
location: https://synbiohub.org/public/igem/BBa_K1618036/annotation2462166/range2462166/1
component: BBa_K1618004

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type: Range
persistent identity: range2462166
display id: range2462166
version: 1
direction: direction/reverse
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BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462167
display id: annotation2462167
version: 1
top level: BBa_K1618036
location: https://synbiohub.org/public/igem/BBa_K1618036/annotation2462167/range2462167/1
component: BBa_K1618037

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type: Range
persistent identity: range2462167
display id: range2462167
version: 1
direction: direction/reverse
top level: BBa_K1618036
start: 2710
end: 2921
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BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462164
display id: annotation2462164
version: 1
top level: BBa_K1618036
location: https://synbiohub.org/public/igem/BBa_K1618036/annotation2462164/range2462164/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618036/annotation2462164/range2462164/1
type: Range
persistent identity: range2462164
display id: range2462164
version: 1
direction: direction/reverse
top level: BBa_K1618036
start: 1
end: 1432
orientation: inline

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462165
display id: annotation2462165
version: 1
top level: BBa_K1618036
location: https://synbiohub.org/public/igem/BBa_K1618036/annotation2462165/range2462165/1
component: BBa_K1618028

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type: Range
persistent identity: range2462165
display id: range2462165
version: 1
direction: direction/reverse
top level: BBa_K1618036
start: 1439
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BBa_K1554002
type: ComponentDefinition
persistent identity: BBa K1554002
display id: BBa_K1554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HarFAR
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fatty alcohol reductase
mutable notes: BioBrick compatible
mutable provenance: It comes from Helicoverpa armigera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554002_sequence/1

BBa_K1467203
type: ComponentDefinition
persistent identity: BBa K1467203
display id: BBa_K1467203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Bax - cell death inducer
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:35:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BAX coding sequence (Bcl-2-associated X) protein, a pro-apoptotic member of the Bcl-2 protein family which induces cell death. The part has been made compatible with the GoldenGate MoClo Assembly Standard and is free from BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Mus musculus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467203_sequence/1

BBa_K1618037
type: ComponentDefinition
persistent identity: BBa K1618037
display id: BBa_K1618037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s Terminator
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:58:16Z
discontinued: false
dominant: false
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: 35s Terminator
mutable notes: None
mutable provenance: Cauliflower mosaic virus 35s gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1618037_sequence/1

BBa_K1742006
type: ComponentDefinition
persistent identity: BBa K1742006
display id: BBa_K1742006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BxBI Reporter (attB:T35S:attP:OmegaUTR)
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:53:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is a CDS of a bxb1 reporter. It is composed by a terminator T35s flanked by an attB recognition site and an attP recognition site
mutable notes: It is plant codon optimized
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1742006_sequence/1

BBa_K1467205
type: ComponentDefinition
persistent identity: BBa K1467205
display id: BBa_K1467205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AvrBS3 TAL Effector - Induces BS3
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:38:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TALE that induces the BS3 promoter. The part is compatible with the GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences) Codon-optimised for expression in dicotyledenous plants
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants
mutable provenance: Xanthomonas vesicatoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1467205_sequence/1

BBa_K2017011
type: ComponentDefinition
persistent identity: BBa K2017011
display id: BBa_K2017011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s:5' + TFL consense + SAGTI-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:15:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s with a 5' region (BBa_K2017002), 20 nucleotides of the TFL consensus gene of Clemenules (clementine orange), the reporter luciferase with SAGTI linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the TFL fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.
mutable provenance: This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K2017002
component: BBa_K1484215
component: BBa_K2017003
sequence annotation: BBa_K1484215
sequence annotation: BBa_K2017002
sequence annotation: BBa_K2017003
sequence: https://synbiohub.org/public/igem/BBa_K2017011_sequence/1

BBa_K2017002
type: Component
persistent identity: component2487436
display id: component2487436
version: 1
top level: BBa_K2017011
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017002/1

BBa_K1484215
type: Component
persistent identity: component2487442
display id: component2487442
version: 1
top level: BBa_K2017011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K2017003
type: Component
persistent identity: component2487439
display id: component2487439
version: 1
top level: BBa_K2017011
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017003/1

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487442
display id: annotation2487442
version: 1
top level: BBa_K2017011
location: https://synbiohub.org/public/igem/BBa_K2017011/annotation2487442/range2487442/1
component: BBa_K1484215

https://synbiohub.org/public/igem/BBa_K2017011/annotation2487442/range2487442/1
type: Range
persistent identity: range2487442
display id: range2487442
version: 1
direction: direction/reverse
top level: BBa_K2017011
start: 2765
end: 3057
orientation: inline

BBa_K2017002
type: SequenceAnnotation
persistent identity: annotation2487436
display id: annotation2487436
version: 1
top level: BBa_K2017011
location: https://synbiohub.org/public/igem/BBa_K2017011/annotation2487436/range2487436/1
component: BBa_K2017002

https://synbiohub.org/public/igem/BBa_K2017011/annotation2487436/range2487436/1
type: Range
persistent identity: range2487436
display id: range2487436
version: 1
direction: direction/reverse
top level: BBa_K2017011
start: 1
end: 1081
orientation: inline

BBa_K2017003
type: SequenceAnnotation
persistent identity: annotation2487439
display id: annotation2487439
version: 1
top level: BBa_K2017011
location: https://synbiohub.org/public/igem/BBa_K2017011/annotation2487439/range2487439/1
component: BBa_K2017003

https://synbiohub.org/public/igem/BBa_K2017011/annotation2487439/range2487439/1
type: Range
persistent identity: range2487439
display id: range2487439
version: 1
direction: direction/reverse
top level: BBa_K2017011
start: 1090
end: 2756
orientation: inline

BBa_K1742005
type: ComponentDefinition
persistent identity: BBa K1742005
display id: BBa_K1742005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BxBI integrase
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:52:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: From mycobacterium phage, it is a CDS of bxb1 integrase from serine recombinase superfamilie. It is composed by two exons and an intron from Ricinus Communis which increases integrase effciency.
mutable notes: It is codon optimized for plants
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742005_sequence/1

BBa_K1742008
type: ComponentDefinition
persistent identity: BBa K1742008
display id: BBa_K1742008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhiC31 Reporter attP:T35S:attB:omegaUTR
creator: Ruben Escriba Piera
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:53:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26673
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The part consists of a T35S terminator flanked by BxbI recognition sites. When the integrase is functionally expressed, it makes double strand breaks in the recognition sites so that the terminator is removed.
mutable notes: This part was designed and sent for synthesis. It is plant codon optimized
mutable provenance: The reporter was specifically designed for its use in plants
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1742008_sequence/1

BBa_K2017012
type: ComponentDefinition
persistent identity: BBa K2017012
display id: BBa_K2017012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + TFL consense + RSIAT-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:15:51Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s (BBa_K1537015), 20 nucleotides of the TFL consensus gene of Clemenules (clementine orange), the reporter luciferase with RSIAT linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the TFL fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.
mutable provenance: This part was obtained using GoldenBraid assembly. The TFL consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1484215
component: BBa_K2017004
component: BBa_K1537015
sequence annotation: BBa_K1484215
sequence annotation: BBa_K1537015
sequence annotation: BBa_K2017004
sequence: https://synbiohub.org/public/igem/BBa_K2017012_sequence/1

BBa_K1484215
type: Component
persistent identity: component2487453
display id: component2487453
version: 1
top level: BBa_K2017012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K2017004
type: Component
persistent identity: component2487450
display id: component2487450
version: 1
top level: BBa_K2017012
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017004/1

BBa_K1537015
type: Component
persistent identity: component2487446
display id: component2487446
version: 1
top level: BBa_K2017012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487453
display id: annotation2487453
version: 1
top level: BBa_K2017012
location: https://synbiohub.org/public/igem/BBa_K2017012/annotation2487453/range2487453/1
component: BBa_K1484215

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type: Range
persistent identity: range2487453
display id: range2487453
version: 1
direction: direction/reverse
top level: BBa_K2017012
start: 2544
end: 2836
orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487446
display id: annotation2487446
version: 1
top level: BBa_K2017012
location: https://synbiohub.org/public/igem/BBa_K2017012/annotation2487446/range2487446/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K2017012/annotation2487446/range2487446/1
type: Range
persistent identity: range2487446
display id: range2487446
version: 1
direction: direction/reverse
top level: BBa_K2017012
start: 1
end: 860
orientation: inline

BBa_K2017004
type: SequenceAnnotation
persistent identity: annotation2487450
display id: annotation2487450
version: 1
top level: BBa_K2017012
location: https://synbiohub.org/public/igem/BBa_K2017012/annotation2487450/range2487450/1
component: BBa_K2017004

https://synbiohub.org/public/igem/BBa_K2017012/annotation2487450/range2487450/1
type: Range
persistent identity: range2487450
display id: range2487450
version: 1
direction: direction/reverse
top level: BBa_K2017012
start: 869
end: 2535
orientation: inline

BBa_K2017009
type: ComponentDefinition
persistent identity: BBa K2017009
display id: BBa_K2017009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + Ga20ox consense + AEK-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:04:17Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s (BBa_K1537015), 20 nucleotides of the Ga20ox consensus gene of Oryza sativa (rice), the reporter luciferase with AEK linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the Ga20ox fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.
mutable provenance: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1484215
component: BBa_K1537015
component: BBa_K2017005
sequence annotation: BBa_K1484215
sequence annotation: BBa_K1537015
sequence annotation: BBa_K2017005
sequence: https://synbiohub.org/public/igem/BBa_K2017009_sequence/1

BBa_K1484215
type: Component
persistent identity: component2487422
display id: component2487422
version: 1
top level: BBa_K2017009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K1537015
type: Component
persistent identity: component2487415
display id: component2487415
version: 1
top level: BBa_K2017009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K2017005
type: Component
persistent identity: component2487419
display id: component2487419
version: 1
top level: BBa_K2017009
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017005/1

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487422
display id: annotation2487422
version: 1
top level: BBa_K2017009
location: https://synbiohub.org/public/igem/BBa_K2017009/annotation2487422/range2487422/1
component: BBa_K1484215

https://synbiohub.org/public/igem/BBa_K2017009/annotation2487422/range2487422/1
type: Range
persistent identity: range2487422
display id: range2487422
version: 1
direction: direction/reverse
top level: BBa_K2017009
start: 2580
end: 2872
orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487415
display id: annotation2487415
version: 1
top level: BBa_K2017009
location: https://synbiohub.org/public/igem/BBa_K2017009/annotation2487415/range2487415/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K2017009/annotation2487415/range2487415/1
type: Range
persistent identity: range2487415
display id: range2487415
version: 1
direction: direction/reverse
top level: BBa_K2017009
start: 1
end: 860
orientation: inline

BBa_K2017005
type: SequenceAnnotation
persistent identity: annotation2487419
display id: annotation2487419
version: 1
top level: BBa_K2017009
location: https://synbiohub.org/public/igem/BBa_K2017009/annotation2487419/range2487419/1
component: BBa_K2017005

https://synbiohub.org/public/igem/BBa_K2017009/annotation2487419/range2487419/1
type: Range
persistent identity: range2487419
display id: range2487419
version: 1
direction: direction/reverse
top level: BBa_K2017009
start: 869
end: 2571
orientation: inline

BBa_K1618032
type: ComponentDefinition
persistent identity: BBa K1618032
display id: BBa_K1618032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 4 -Fused to GFP -Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:56:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates starch with a GFP tag for visualisation of localisation
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1467101
component: BBa_K1618037
component: BBa_K1618004
component: BBa_K1618028
component: BBa_K1467204
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1618028
sequence annotation: BBa_K1467204
sequence annotation: BBa_K1618004
sequence: https://synbiohub.org/public/igem/BBa_K1618032_sequence/1

BBa_K1467101
type: Component
persistent identity: component2462186
display id: component2462186
version: 1
top level: BBa_K1618032
access: public
definition: BBa_K1467101

BBa_K1618037
type: Component
persistent identity: component2462190
display id: component2462190
version: 1
top level: BBa_K1618032
access: public
definition: BBa_K1618037

BBa_K1618004
type: Component
persistent identity: component2462188
display id: component2462188
version: 1
top level: BBa_K1618032
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618004/1

BBa_K1618028
type: Component
persistent identity: component2462187
display id: component2462187
version: 1
top level: BBa_K1618032
access: public
definition: BBa_K1618028

BBa_K1467204
type: Component
persistent identity: component2462189
display id: component2462189
version: 1
top level: BBa_K1618032
access: public
definition: BBa_K1467204

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462186
display id: annotation2462186
version: 1
top level: BBa_K1618032
location: https://synbiohub.org/public/igem/BBa_K1618032/annotation2462186/range2462186/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618032/annotation2462186/range2462186/1
type: Range
persistent identity: range2462186
display id: range2462186
version: 1
direction: direction/reverse
top level: BBa_K1618032
start: 1
end: 1432
orientation: inline

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462190
display id: annotation2462190
version: 1
top level: BBa_K1618032
location: https://synbiohub.org/public/igem/BBa_K1618032/annotation2462190/range2462190/1
component: BBa_K1618037

https://synbiohub.org/public/igem/BBa_K1618032/annotation2462190/range2462190/1
type: Range
persistent identity: range2462190
display id: range2462190
version: 1
direction: direction/reverse
top level: BBa_K1618032
start: 3448
end: 3659
orientation: inline

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462187
display id: annotation2462187
version: 1
top level: BBa_K1618032
location: https://synbiohub.org/public/igem/BBa_K1618032/annotation2462187/range2462187/1
component: BBa_K1618028

https://synbiohub.org/public/igem/BBa_K1618032/annotation2462187/range2462187/1
type: Range
persistent identity: range2462187
display id: range2462187
version: 1
direction: direction/reverse
top level: BBa_K1618032
start: 1439
end: 1615
orientation: inline

BBa_K1467204
type: SequenceAnnotation
persistent identity: annotation2462189
display id: annotation2462189
version: 1
top level: BBa_K1618032
location: https://synbiohub.org/public/igem/BBa_K1618032/annotation2462189/range2462189/1
component: BBa_K1467204

https://synbiohub.org/public/igem/BBa_K1618032/annotation2462189/range2462189/1
type: Range
persistent identity: range2462189
display id: range2462189
version: 1
direction: direction/reverse
top level: BBa_K1618032
start: 2710
end: 3439
orientation: inline

BBa_K1618004
type: SequenceAnnotation
persistent identity: annotation2462188
display id: annotation2462188
version: 1
top level: BBa_K1618032
location: https://synbiohub.org/public/igem/BBa_K1618032/annotation2462188/range2462188/1
component: BBa_K1618004

https://synbiohub.org/public/igem/BBa_K1618032/annotation2462188/range2462188/1
type: Range
persistent identity: range2462188
display id: range2462188
version: 1
direction: direction/reverse
top level: BBa_K1618032
start: 1622
end: 2701
orientation: inline

BBa_K1618031
type: ComponentDefinition
persistent identity: BBa K1618031
display id: BBa_K1618031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 3 -Fused to GFP -Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:56:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates starch with GFP tag for visualisation of localisation
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618003
component: BBa_K1618028
component: BBa_K1467101
component: BBa_K1618037
component: BBa_K1467204
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618003
sequence annotation: BBa_K1467204
sequence annotation: BBa_K1618028
sequence annotation: BBa_K1618037
sequence: https://synbiohub.org/public/igem/BBa_K1618031_sequence/1

BBa_K1618003
type: Component
persistent identity: component2462180
display id: component2462180
version: 1
top level: BBa_K1618031
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618003/1

BBa_K1618028
type: Component
persistent identity: component2462179
display id: component2462179
version: 1
top level: BBa_K1618031
access: public
definition: BBa_K1618028

BBa_K1467101
type: Component
persistent identity: component2462178
display id: component2462178
version: 1
top level: BBa_K1618031
access: public
definition: BBa_K1467101

BBa_K1618037
type: Component
persistent identity: component2462182
display id: component2462182
version: 1
top level: BBa_K1618031
access: public
definition: BBa_K1618037

BBa_K1467204
type: Component
persistent identity: component2462181
display id: component2462181
version: 1
top level: BBa_K1618031
access: public
definition: BBa_K1467204

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462178
display id: annotation2462178
version: 1
top level: BBa_K1618031
location: https://synbiohub.org/public/igem/BBa_K1618031/annotation2462178/range2462178/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618031/annotation2462178/range2462178/1
type: Range
persistent identity: range2462178
display id: range2462178
version: 1
direction: direction/reverse
top level: BBa_K1618031
start: 1
end: 1432
orientation: inline

BBa_K1618003
type: SequenceAnnotation
persistent identity: annotation2462180
display id: annotation2462180
version: 1
top level: BBa_K1618031
location: https://synbiohub.org/public/igem/BBa_K1618031/annotation2462180/range2462180/1
component: BBa_K1618003

https://synbiohub.org/public/igem/BBa_K1618031/annotation2462180/range2462180/1
type: Range
persistent identity: range2462180
display id: range2462180
version: 1
direction: direction/reverse
top level: BBa_K1618031
start: 1622
end: 2527
orientation: inline

BBa_K1467204
type: SequenceAnnotation
persistent identity: annotation2462181
display id: annotation2462181
version: 1
top level: BBa_K1618031
location: https://synbiohub.org/public/igem/BBa_K1618031/annotation2462181/range2462181/1
component: BBa_K1467204

https://synbiohub.org/public/igem/BBa_K1618031/annotation2462181/range2462181/1
type: Range
persistent identity: range2462181
display id: range2462181
version: 1
direction: direction/reverse
top level: BBa_K1618031
start: 2536
end: 3265
orientation: inline

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462179
display id: annotation2462179
version: 1
top level: BBa_K1618031
location: https://synbiohub.org/public/igem/BBa_K1618031/annotation2462179/range2462179/1
component: BBa_K1618028

https://synbiohub.org/public/igem/BBa_K1618031/annotation2462179/range2462179/1
type: Range
persistent identity: range2462179
display id: range2462179
version: 1
direction: direction/reverse
top level: BBa_K1618031
start: 1439
end: 1615
orientation: inline

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462182
display id: annotation2462182
version: 1
top level: BBa_K1618031
location: https://synbiohub.org/public/igem/BBa_K1618031/annotation2462182/range2462182/1
component: BBa_K1618037

https://synbiohub.org/public/igem/BBa_K1618031/annotation2462182/range2462182/1
type: Range
persistent identity: range2462182
display id: range2462182
version: 1
direction: direction/reverse
top level: BBa_K1618031
start: 3274
end: 3485
orientation: inline

BBa_K1618029
type: ComponentDefinition
persistent identity: BBa K1618029
display id: BBa_K1618029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 1 -Fused to GFP -Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:55:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates starch in plants with a GFP tag for localisation
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618037
component: BBa_K1467101
component: BBa_K1467204
component: BBa_K1618001
component: BBa_K1618028
sequence annotation: BBa_K1618001
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1467204
sequence annotation: BBa_K1618028
sequence: https://synbiohub.org/public/igem/BBa_K1618029_sequence/1

BBa_K1618037
type: Component
persistent identity: component2462172
display id: component2462172
version: 1
top level: BBa_K1618029
access: public
definition: BBa_K1618037

BBa_K1467101
type: Component
persistent identity: component2462168
display id: component2462168
version: 1
top level: BBa_K1618029
access: public
definition: BBa_K1467101

BBa_K1467204
type: Component
persistent identity: component2462171
display id: component2462171
version: 1
top level: BBa_K1618029
access: public
definition: BBa_K1467204

BBa_K1618001
type: Component
persistent identity: component2462170
display id: component2462170
version: 1
top level: BBa_K1618029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618001/1

BBa_K1618028
type: Component
persistent identity: component2462169
display id: component2462169
version: 1
top level: BBa_K1618029
access: public
definition: BBa_K1618028

BBa_K1618001
type: SequenceAnnotation
persistent identity: annotation2462170
display id: annotation2462170
version: 1
top level: BBa_K1618029
location: https://synbiohub.org/public/igem/BBa_K1618029/annotation2462170/range2462170/1
component: BBa_K1618001

https://synbiohub.org/public/igem/BBa_K1618029/annotation2462170/range2462170/1
type: Range
persistent identity: range2462170
display id: range2462170
version: 1
direction: direction/reverse
top level: BBa_K1618029
start: 1622
end: 2176
orientation: inline

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462168
display id: annotation2462168
version: 1
top level: BBa_K1618029
location: https://synbiohub.org/public/igem/BBa_K1618029/annotation2462168/range2462168/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618029/annotation2462168/range2462168/1
type: Range
persistent identity: range2462168
display id: range2462168
version: 1
direction: direction/reverse
top level: BBa_K1618029
start: 1
end: 1432
orientation: inline

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462172
display id: annotation2462172
version: 1
top level: BBa_K1618029
location: https://synbiohub.org/public/igem/BBa_K1618029/annotation2462172/range2462172/1
component: BBa_K1618037

https://synbiohub.org/public/igem/BBa_K1618029/annotation2462172/range2462172/1
type: Range
persistent identity: range2462172
display id: range2462172
version: 1
direction: direction/reverse
top level: BBa_K1618029
start: 2923
end: 3134
orientation: inline

BBa_K1467204
type: SequenceAnnotation
persistent identity: annotation2462171
display id: annotation2462171
version: 1
top level: BBa_K1618029
location: https://synbiohub.org/public/igem/BBa_K1618029/annotation2462171/range2462171/1
component: BBa_K1467204

https://synbiohub.org/public/igem/BBa_K1618029/annotation2462171/range2462171/1
type: Range
persistent identity: range2462171
display id: range2462171
version: 1
direction: direction/reverse
top level: BBa_K1618029
start: 2185
end: 2914
orientation: inline

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462169
display id: annotation2462169
version: 1
top level: BBa_K1618029
location: https://synbiohub.org/public/igem/BBa_K1618029/annotation2462169/range2462169/1
component: BBa_K1618028

https://synbiohub.org/public/igem/BBa_K1618029/annotation2462169/range2462169/1
type: Range
persistent identity: range2462169
display id: range2462169
version: 1
direction: direction/reverse
top level: BBa_K1618029
start: 1439
end: 1615
orientation: inline

BBa_K1742001
type: ComponentDefinition
persistent identity: BBa K1742001
display id: BBa_K1742001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Entertoxin LTB
creator: Ruben Escriba Piera
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T01:50:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Diarrheal diseases caused by Vibrio cholera and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). The E. coli heat-labile enterotoxin (LT) is the major ETEC pathogenic factor. LT subunit B (LTB) is of special interest because its immunogenicity, adjuvant activity and lack of toxicity make it a viable antigen to vaccinate against ETEC diarrhea.
mutable notes: The protein consists of the E. coli enterotoxin beta subunit. It is codon optimized for plants and contains a C-terminus 6X Hys tag in order to detected by anti-Hys antibodies.
mutable provenance: The LTB protein was designed based on a publication that showed that it can be expressed in Nicotiana Benthamiana plants.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742001_sequence/1

BBa_K1742013
type: ComponentDefinition
persistent identity: BBa K1742013
display id: BBa_K1742013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S:PhiC31:T35S - 35S:ReporterPhiC31::GFP:T35S
creator: Ruben Escriba Piera
created: 2015-09-09T11:00:00Z
modified: 2015-12-16T01:55:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26673
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.
mutable notes: Transcriptional units assembled with GoldenBraid
mutable provenance: Parts assembled that exist already
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1742013_sequence/1

BBa_K2017000
type: ComponentDefinition
persistent identity: BBa K2017000
display id: BBa_K2017000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-split Cas9 + DnaE C-intein
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-02T11:00:00Z
modified: 2016-10-23T08:31:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Half of the SpCas9 protein fused to DnaE C-intein. The active full-lenght spCas9 can be recovered by using the other half of the protein, N-split Cas9 + DnaE N-intein. Inteins are used to fuse both parts of the protein, as they interact and splice, leaving the active protein.
mutable notes: As the part needs to be inserted in a viral vector, the overhangs have to be designed accordingly to the viral vector that is going to be used. The synthesis of C-split Cas9 could not be made in one gBlock, as it is too big. Hence, the half protein had to be splitted in two other parts for its synthesis.
mutable provenance: The protein domain is synthesized with the intein.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: C-split Cas9
sequence annotation: DnaE C-intein
sequence: https://synbiohub.org/public/igem/BBa_K2017000_sequence/1

C-split Cas9
type: SequenceAnnotation
persistent identity: annotation2486019
display id: annotation2486019
version: 1
top level: BBa_K2017000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2017000/annotation2486019/range2486019/1

https://synbiohub.org/public/igem/BBa_K2017000/annotation2486019/range2486019/1
type: Range
persistent identity: range2486019
display id: range2486019
version: 1
direction: direction/forward
top level: BBa_K2017000
start: 109
end: 2358
orientation: inline

DnaE C-intein
type: SequenceAnnotation
persistent identity: annotation2486018
display id: annotation2486018
version: 1
top level: BBa_K2017000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017000/annotation2486018/range2486018/1

https://synbiohub.org/public/igem/BBa_K2017000/annotation2486018/range2486018/1
type: Range
persistent identity: range2486018
display id: range2486018
version: 1
direction: direction/forward
top level: BBa_K2017000
start: 1
end: 108
orientation: inline

BBa_K1742003
type: ComponentDefinition
persistent identity: BBa K1742003
display id: BBa_K1742003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145K
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: From Pectinidae sp. It is a CDS of green fluorescent protein Dronpa.
mutable notes: It is codon optimized for plants.
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742003_sequence/1

BBa_K1467102
type: ComponentDefinition
persistent identity: BBa K1467102
display id: BBa_K1467102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible BS3 promoter + 5
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:36:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: BS3 is a plant specific promoter which is induced by the TALE AvrBS3. This part is compatible with the GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Capsicum annum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467102_sequence/1

BBa_K1467101
type: ComponentDefinition
persistent identity: BBa K1467101
display id: BBa_K1467101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible 35s Promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:36:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: 35S is a plant specific promoter obtained from Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments. The part is compatible with GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Cauliflower Mosaic Virus (CaMV)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467101_sequence/1

BBa_K1467204
type: ComponentDefinition
persistent identity: BBa K1467204
display id: BBa_K1467204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Green Flourescent Protein (GFP)
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:05:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences. Fluoresces under UV light when the gene is expressed.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
mutable provenance: Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467204_sequence/1

BBa_K1554005
type: ComponentDefinition
persistent identity: BBa K1554005
display id: BBa_K1554005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue biosafety module for plants
creator: Valencia_UPV team members
created: 2014-09-28T11:00:00Z
modified: 2015-06-09T09:45:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the blue chromoprotein AmilCP, so that plants can be visually differentiated from non-transgenic plants.
mutable notes: The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.
mutable provenance: TA29 promoter [http://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] Barnase [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] AmilGFP [Waiting for Norwich team part name in registry] P35s [http://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: AmilCP
sequence annotation: T35S
sequence annotation: P35s
sequence annotation: T35s
sequence annotation: TA29 promoter
sequence annotation: barnase
sequence: https://synbiohub.org/public/igem/BBa_K1554005_sequence/1

AmilCP
type: SequenceAnnotation
persistent identity: annotation2389555
display id: annotation2389555
version: 1
top level: BBa_K1554005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389555/range2389555/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389555/range2389555/1
type: Range
persistent identity: range2389555
display id: range2389555
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 2469
end: 3131
orientation: inline

T35S
type: SequenceAnnotation
persistent identity: annotation2389556
display id: annotation2389556
version: 1
top level: BBa_K1554005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389556/range2389556/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389556/range2389556/1
type: Range
persistent identity: range2389556
display id: range2389556
version: 1
direction: direction/reverse
top level: BBa_K1554005
start: 3136
end: 3361
orientation: inline

P35s
type: SequenceAnnotation
persistent identity: annotation2389541
display id: annotation2389541
version: 1
top level: BBa_K1554005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389541/range2389541/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389541/range2389541/1
type: Range
persistent identity: range2389541
display id: range2389541
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 1438
end: 2464
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2389540
display id: annotation2389540
version: 1
top level: BBa_K1554005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389540/range2389540/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389540/range2389540/1
type: Range
persistent identity: range2389540
display id: range2389540
version: 1
direction: direction/reverse
top level: BBa_K1554005
start: 1200
end: 1425
orientation: inline

TA29 promoter
type: SequenceAnnotation
persistent identity: annotation2389538
display id: annotation2389538
version: 1
top level: BBa_K1554005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389538/range2389538/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389538/range2389538/1
type: Range
persistent identity: range2389538
display id: range2389538
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 4
end: 858
orientation: inline

barnase
type: SequenceAnnotation
persistent identity: annotation2389539
display id: annotation2389539
version: 1
top level: BBa_K1554005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389539/range2389539/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389539/range2389539/1
type: Range
persistent identity: range2389539
display id: range2389539
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 863
end: 1195
orientation: inline

BBa_K1618034
type: ComponentDefinition
persistent identity: BBa K1618034
display id: BBa_K1618034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 2 Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:57:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates Starch
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618037
component: BBa_K1618028
component: BBa_K1467101
component: BBa_K1618002
sequence annotation: BBa_K1618002
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618028
sequence: https://synbiohub.org/public/igem/BBa_K1618034_sequence/1

BBa_K1618037
type: Component
persistent identity: component2462157
display id: component2462157
version: 1
top level: BBa_K1618034
access: public
definition: BBa_K1618037

BBa_K1618028
type: Component
persistent identity: component2462155
display id: component2462155
version: 1
top level: BBa_K1618034
access: public
definition: BBa_K1618028

BBa_K1467101
type: Component
persistent identity: component2462154
display id: component2462154
version: 1
top level: BBa_K1618034
access: public
definition: BBa_K1467101

BBa_K1618002
type: Component
persistent identity: component2462156
display id: component2462156
version: 1
top level: BBa_K1618034
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618002/1

BBa_K1618002
type: SequenceAnnotation
persistent identity: annotation2462156
display id: annotation2462156
version: 1
top level: BBa_K1618034
location: https://synbiohub.org/public/igem/BBa_K1618034/annotation2462156/range2462156/1
component: BBa_K1618002

https://synbiohub.org/public/igem/BBa_K1618034/annotation2462156/range2462156/1
type: Range
persistent identity: range2462156
display id: range2462156
version: 1
direction: direction/reverse
top level: BBa_K1618034
start: 1622
end: 2449
orientation: inline

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462157
display id: annotation2462157
version: 1
top level: BBa_K1618034
location: https://synbiohub.org/public/igem/BBa_K1618034/annotation2462157/range2462157/1
component: BBa_K1618037

https://synbiohub.org/public/igem/BBa_K1618034/annotation2462157/range2462157/1
type: Range
persistent identity: range2462157
display id: range2462157
version: 1
direction: direction/reverse
top level: BBa_K1618034
start: 2458
end: 2669
orientation: inline

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462154
display id: annotation2462154
version: 1
top level: BBa_K1618034
location: https://synbiohub.org/public/igem/BBa_K1618034/annotation2462154/range2462154/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618034/annotation2462154/range2462154/1
type: Range
persistent identity: range2462154
display id: range2462154
version: 1
direction: direction/reverse
top level: BBa_K1618034
start: 1
end: 1432
orientation: inline

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462155
display id: annotation2462155
version: 1
top level: BBa_K1618034
location: https://synbiohub.org/public/igem/BBa_K1618034/annotation2462155/range2462155/1
component: BBa_K1618028

https://synbiohub.org/public/igem/BBa_K1618034/annotation2462155/range2462155/1
type: Range
persistent identity: range2462155
display id: range2462155
version: 1
direction: direction/reverse
top level: BBa_K1618034
start: 1439
end: 1615
orientation: inline

BBa_K1554001
type: ComponentDefinition
persistent identity: BBa K1554001
display id: BBa_K1554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AtrΔ11
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Delta11 desaturase
mutable notes: BioBrick compatible
mutable provenance: It comes from Amyelois transitella.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554001_sequence/1

BBa_K1618033
type: ComponentDefinition
persistent identity: BBa K1618033
display id: BBa_K1618033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 1 Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:57:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates Starch
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1618037
component: BBa_K1467101
component: BBa_K1618028
component: BBa_K1618001
sequence annotation: BBa_K1618028
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1467101
sequence annotation: BBa_K1618001
sequence: https://synbiohub.org/public/igem/BBa_K1618033_sequence/1

BBa_K1618037
type: Component
persistent identity: component2462153
display id: component2462153
version: 1
top level: BBa_K1618033
access: public
definition: BBa_K1618037

BBa_K1467101
type: Component
persistent identity: component2462150
display id: component2462150
version: 1
top level: BBa_K1618033
access: public
definition: BBa_K1467101

BBa_K1618028
type: Component
persistent identity: component2462151
display id: component2462151
version: 1
top level: BBa_K1618033
access: public
definition: BBa_K1618028

BBa_K1618001
type: Component
persistent identity: component2462152
display id: component2462152
version: 1
top level: BBa_K1618033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618001/1

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462151
display id: annotation2462151
version: 1
top level: BBa_K1618033
location: https://synbiohub.org/public/igem/BBa_K1618033/annotation2462151/range2462151/1
component: BBa_K1618028

https://synbiohub.org/public/igem/BBa_K1618033/annotation2462151/range2462151/1
type: Range
persistent identity: range2462151
display id: range2462151
version: 1
direction: direction/reverse
top level: BBa_K1618033
start: 1439
end: 1615
orientation: inline

BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462153
display id: annotation2462153
version: 1
top level: BBa_K1618033
location: https://synbiohub.org/public/igem/BBa_K1618033/annotation2462153/range2462153/1
component: BBa_K1618037

https://synbiohub.org/public/igem/BBa_K1618033/annotation2462153/range2462153/1
type: Range
persistent identity: range2462153
display id: range2462153
version: 1
direction: direction/reverse
top level: BBa_K1618033
start: 2185
end: 2396
orientation: inline

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462150
display id: annotation2462150
version: 1
top level: BBa_K1618033
location: https://synbiohub.org/public/igem/BBa_K1618033/annotation2462150/range2462150/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1618033/annotation2462150/range2462150/1
type: Range
persistent identity: range2462150
display id: range2462150
version: 1
direction: direction/reverse
top level: BBa_K1618033
start: 1
end: 1432
orientation: inline

BBa_K1618001
type: SequenceAnnotation
persistent identity: annotation2462152
display id: annotation2462152
version: 1
top level: BBa_K1618033
location: https://synbiohub.org/public/igem/BBa_K1618033/annotation2462152/range2462152/1
component: BBa_K1618001

https://synbiohub.org/public/igem/BBa_K1618033/annotation2462152/range2462152/1
type: Range
persistent identity: range2462152
display id: range2462152
version: 1
direction: direction/reverse
top level: BBa_K1618033
start: 1622
end: 2176
orientation: inline

BBa_K1742002
type: ComponentDefinition
persistent identity: BBa K1742002
display id: BBa_K1742002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:00Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
mutable notes: It is codon optimized for plants
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742002_sequence/1

BBa_K1554004
type: ComponentDefinition
persistent identity: BBa K1554004
display id: BBa_K1554004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow biosafety module for plants
creator: Valencia_UPV team members
created: 2014-09-28T11:00:00Z
modified: 2015-06-09T09:45:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.
mutable notes: The assembly was performed by GoldenBraid assembly. The barnase was ampliified by PCR from [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.
mutable provenance: TA29 promoter [http://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] Barnase [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] AmilGFP [Waiting for Norwich team part name in registry] P35s [http://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: P35s
sequence annotation: T35s
sequence annotation: TA29 promoter
sequence annotation: Barnase
sequence annotation: AmilGFP
sequence: https://synbiohub.org/public/igem/BBa_K1554004_sequence/1

P35s
type: SequenceAnnotation
persistent identity: annotation2388988
display id: annotation2388988
version: 1
top level: BBa_K1554004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388988/range2388988/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388988/range2388988/1
type: Range
persistent identity: range2388988
display id: range2388988
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 1438
end: 2464
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2388987
display id: annotation2388987
version: 1
top level: BBa_K1554004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388987/range2388987/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388987/range2388987/1
type: Range
persistent identity: range2388987
display id: range2388987
version: 1
direction: direction/reverse
top level: BBa_K1554004
start: 1200
end: 1425
orientation: inline

TA29 promoter
type: SequenceAnnotation
persistent identity: annotation2388985
display id: annotation2388985
version: 1
top level: BBa_K1554004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388985/range2388985/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388985/range2388985/1
type: Range
persistent identity: range2388985
display id: range2388985
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 4
end: 858
orientation: inline

Barnase
type: SequenceAnnotation
persistent identity: annotation2388986
display id: annotation2388986
version: 1
top level: BBa_K1554004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388986/range2388986/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388986/range2388986/1
type: Range
persistent identity: range2388986
display id: range2388986
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 863
end: 1195
orientation: inline

AmilGFP
type: SequenceAnnotation
persistent identity: annotation2388989
display id: annotation2388989
version: 1
top level: BBa_K1554004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388989/range2388989/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388989/range2388989/1
type: Range
persistent identity: range2388989
display id: range2388989
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 2469
end: 3161
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2388984
display id: annotation2388984
version: 1
top level: BBa_K1554004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388984/range2388984/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388984/range2388984/1
type: Range
persistent identity: range2388984
display id: range2388984
version: 1
direction: direction/reverse
top level: BBa_K1554004
start: 3166
end: 3391
orientation: inline

BBa_K2017013
type: ComponentDefinition
persistent identity: BBa K2017013
display id: BBa_K2017013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + TFL consense + AEK-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:16:14Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s (BBa_K1537015), 20 nucleotides of the TFL consensus gene of Clemenules (clementine orange), the reporter luciferase with AEK linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the TFL fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.
mutable provenance: This part was obtained using GoldenBraid assembly. The TFL consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537015
component: BBa_K2017005
component: BBa_K1484215
sequence annotation: BBa_K1537015
sequence annotation: BBa_K1484215
sequence annotation: BBa_K2017005
sequence: https://synbiohub.org/public/igem/BBa_K2017013_sequence/1

BBa_K1537015
type: Component
persistent identity: component2487457
display id: component2487457
version: 1
top level: BBa_K2017013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K2017005
type: Component
persistent identity: component2487461
display id: component2487461
version: 1
top level: BBa_K2017013
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017005/1

BBa_K1484215
type: Component
persistent identity: component2487464
display id: component2487464
version: 1
top level: BBa_K2017013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487457
display id: annotation2487457
version: 1
top level: BBa_K2017013
location: https://synbiohub.org/public/igem/BBa_K2017013/annotation2487457/range2487457/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K2017013/annotation2487457/range2487457/1
type: Range
persistent identity: range2487457
display id: range2487457
version: 1
direction: direction/reverse
top level: BBa_K2017013
start: 1
end: 860
orientation: inline

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487464
display id: annotation2487464
version: 1
top level: BBa_K2017013
location: https://synbiohub.org/public/igem/BBa_K2017013/annotation2487464/range2487464/1
component: BBa_K1484215

https://synbiohub.org/public/igem/BBa_K2017013/annotation2487464/range2487464/1
type: Range
persistent identity: range2487464
display id: range2487464
version: 1
direction: direction/reverse
top level: BBa_K2017013
start: 2580
end: 2872
orientation: inline

BBa_K2017005
type: SequenceAnnotation
persistent identity: annotation2487461
display id: annotation2487461
version: 1
top level: BBa_K2017013
location: https://synbiohub.org/public/igem/BBa_K2017013/annotation2487461/range2487461/1
component: BBa_K2017005

https://synbiohub.org/public/igem/BBa_K2017013/annotation2487461/range2487461/1
type: Range
persistent identity: range2487461
display id: range2487461
version: 1
direction: direction/reverse
top level: BBa_K2017013
start: 869
end: 2571
orientation: inline

BBa_K2017001
type: ComponentDefinition
persistent identity: BBa K2017001
display id: BBa_K2017001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-split Cas9 + DnaE N-intein
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-02T11:00:00Z
modified: 2016-10-23T08:32:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Half of the SpCas9 protein fused to DnaE C-intein. The active full-lenght spCas9 can be recovered by using the other half of the protein, C-split Cas9 + DnaE C-intein. Inteins are used to fuse both parts of the protein, as they interact and splice, leaving the active protein.
mutable notes: As the part needs to be inserted in a viral vector, the overhangs have to be designed accordingly to the viral vector that is going to be used. The synthesis of N-split Cas9 could not be made in one gBlock, as it is too big. Hence, the half protein had to be splitted in two other parts for its synthesis.
mutable provenance: The protein domain is synthesized with the intein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-split Cas9
sequence annotation: DnaE N-intein
sequence: https://synbiohub.org/public/igem/BBa_K2017001_sequence/1

N-split Cas9
type: SequenceAnnotation
persistent identity: annotation2485943
display id: annotation2485943
version: 1
top level: BBa_K2017001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2017001/annotation2485943/range2485943/1

https://synbiohub.org/public/igem/BBa_K2017001/annotation2485943/range2485943/1
type: Range
persistent identity: range2485943
display id: range2485943
version: 1
direction: direction/forward
top level: BBa_K2017001
start: 1
end: 1933
orientation: inline

DnaE N-intein
type: SequenceAnnotation
persistent identity: annotation2485944
display id: annotation2485944
version: 1
top level: BBa_K2017001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2017001/annotation2485944/range2485944/1

https://synbiohub.org/public/igem/BBa_K2017001/annotation2485944/range2485944/1
type: Range
persistent identity: range2485944
display id: range2485944
version: 1
direction: direction/forward
top level: BBa_K2017001
start: 1934
end: 2241
orientation: inline

BBa_K1742009
type: ComponentDefinition
persistent identity: BBa K1742009
display id: BBa_K1742009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35S
creator: Pilar Baldominos
created: 2015-09-06T11:00:00Z
modified: 2015-12-16T01:53:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26679
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.
mutable notes: aaaa
mutable provenance: P35s: BBa_K1537015 Bxb1: BBa_K1742005 Bxb1reporter: BBa_K1742006 GFP: Golden braid 2.0 collection T35s: Golden braid 2.0 collection
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K1537015
sequence annotation: BBa_K1742006
sequence annotation: translation initiation optimized sequence for dicot
sequence annotation: BBa_K1742005
sequence annotation: mass translation enhancer
sequence annotation: 35s promoter
sequence: https://synbiohub.org/public/igem/BBa_K1742009_sequence/1

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2445403
display id: annotation2445403
version: 1
top level: BBa_K1742009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445403/range2445403/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445403/range2445403/1
type: Range
persistent identity: range2445403
display id: range2445403
version: 1
direction: direction/reverse
top level: BBa_K1742009
start: 2105
end: 2964
orientation: inline

BBa_K1742006
type: SequenceAnnotation
persistent identity: annotation2445398
display id: annotation2445398
version: 1
top level: BBa_K1742009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445398/range2445398/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445398/range2445398/1
type: Range
persistent identity: range2445398
display id: range2445398
version: 1
direction: direction/reverse
top level: BBa_K1742009
start: 1
end: 397
orientation: inline

translation initiation optimized sequence for dicot
type: SequenceAnnotation
persistent identity: annotation2445401
display id: annotation2445401
version: 1
top level: BBa_K1742009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445401/range2445401/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445401/range2445401/1
type: Range
persistent identity: range2445401
display id: range2445401
version: 1
direction: direction/forward
top level: BBa_K1742009
start: 2947
end: 2955
orientation: inline

BBa_K1742005
type: SequenceAnnotation
persistent identity: annotation2445399
display id: annotation2445399
version: 1
top level: BBa_K1742009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445399/range2445399/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445399/range2445399/1
type: Range
persistent identity: range2445399
display id: range2445399
version: 1
direction: direction/reverse
top level: BBa_K1742009
start: 404
end: 2096
orientation: inline

mass translation enhancer
type: SequenceAnnotation
persistent identity: annotation2445402
display id: annotation2445402
version: 1
top level: BBa_K1742009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445402/range2445402/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445402/range2445402/1
type: Range
persistent identity: range2445402
display id: range2445402
version: 1
direction: direction/forward
top level: BBa_K1742009
start: 2956
end: 2964
orientation: inline

35s promoter
type: SequenceAnnotation
persistent identity: annotation2445400
display id: annotation2445400
version: 1
top level: BBa_K1742009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1742009/annotation2445400/range2445400/1

https://synbiohub.org/public/igem/BBa_K1742009/annotation2445400/range2445400/1
type: Range
persistent identity: range2445400
display id: range2445400
version: 1
direction: direction/forward
top level: BBa_K1742009
start: 2105
end: 2946
orientation: inline

BBa_K2017014
type: ComponentDefinition
persistent identity: BBa K2017014
display id: BBa_K2017014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + TFL consense + RSIAT-TEV-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:18:25Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s (BBa_K1537015), 20 nucleotides of the TFL consensus gene of Clemenules (clementine orange), the reporter luciferase with RSIAT+TEV linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the TFL fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.
mutable provenance: This part was obtained using GoldenBraid assembly. The TFL consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537015
component: BBa_K1484215
component: BBa_K2017006
sequence annotation: BBa_K1537015
sequence annotation: BBa_K2017006
sequence annotation: BBa_K1484215
sequence: https://synbiohub.org/public/igem/BBa_K2017014_sequence/1

BBa_K1537015
type: Component
persistent identity: component2487468
display id: component2487468
version: 1
top level: BBa_K2017014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1484215
type: Component
persistent identity: component2487475
display id: component2487475
version: 1
top level: BBa_K2017014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K2017006
type: Component
persistent identity: component2487472
display id: component2487472
version: 1
top level: BBa_K2017014
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017006/1

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487468
display id: annotation2487468
version: 1
top level: BBa_K2017014
location: https://synbiohub.org/public/igem/BBa_K2017014/annotation2487468/range2487468/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K2017014/annotation2487468/range2487468/1
type: Range
persistent identity: range2487468
display id: range2487468
version: 1
direction: direction/reverse
top level: BBa_K2017014
start: 1
end: 860
orientation: inline

BBa_K2017006
type: SequenceAnnotation
persistent identity: annotation2487472
display id: annotation2487472
version: 1
top level: BBa_K2017014
location: https://synbiohub.org/public/igem/BBa_K2017014/annotation2487472/range2487472/1
component: BBa_K2017006

https://synbiohub.org/public/igem/BBa_K2017014/annotation2487472/range2487472/1
type: Range
persistent identity: range2487472
display id: range2487472
version: 1
direction: direction/reverse
top level: BBa_K2017014
start: 869
end: 2568
orientation: inline

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487475
display id: annotation2487475
version: 1
top level: BBa_K2017014
location: https://synbiohub.org/public/igem/BBa_K2017014/annotation2487475/range2487475/1
component: BBa_K1484215

https://synbiohub.org/public/igem/BBa_K2017014/annotation2487475/range2487475/1
type: Range
persistent identity: range2487475
display id: range2487475
version: 1
direction: direction/reverse
top level: BBa_K2017014
start: 2577
end: 2869
orientation: inline

BBa_K1554000
type: ComponentDefinition
persistent identity: BBa K1554000
display id: BBa_K1554000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TA29 promoter
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-29T01:38:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anther-specific promoter
mutable notes: BioBrick compatible
mutable provenance: Genomic from Nicotiana tabacco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1554000_sequence/1

AsLOV2pep
type: ComponentDefinition
persistent identity: BBa K1742000
display id: BBa_K1742000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Avena sativa LOV2 domain
creator: Ruben Escriba Piera
created: 2015-09-02T11:00:00Z
modified: 2015-12-16T01:55:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plants and bacteria contain a variety of photoreceptors that activate intracellular signalling in response ti light. Among these, the most extensively studied are LOV (light/oxygen/voltage) domains. On blue light (peak 450nm) aabsorption, the FMN cofactor in the LOV domain forms a covalent bond with a cysteine residue. In the LOV2 domain of plant phototropin, the FMN-cysteine bond formation leads to partial unfolding of the C-terminal alpha-helix from the rest of the LOV2 domain. Strickland et al. created LOV2 fusions capable of light-mediated heterodimerization by fusing the LOV2 domain to peptides that bind to PDZ domains.
mutable notes: The LOv2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on this peptide, the engineered variants of the Erbin PDZ domains would vary in interaction affinity.
mutable provenance: The AsLOV2 fused to a peptide epitope was designed according to the second LOV domain of Avena sativa phototropin (UniprotKB 049003)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742000_sequence/1

BBa_K1618028
type: ComponentDefinition
persistent identity: BBa K1618028
display id: BBa_K1618028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chloroplast Transit Peptide
creator: Leda Coelewij
created: 2015-09-07T11:00:00Z
modified: 2015-12-16T02:53:59Z
discontinued: false
dominant: false
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is synthetic a 5'UTR, omega (Tobacco Mosaic Virus. The chloroplast transit peptide provides subcellular localisation to the chloroplast.
mutable notes: TBD
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1618028_sequence/1

BBa_K1742004
type: ComponentDefinition
persistent identity: BBa K1742004
display id: BBa_K1742004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhiC31 Plant codon optimized
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2015-12-16T01:52:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is a CDS of PhiC31 integrase from Streptomyces phage. It has a nuclear location sequence in the N-terminus.
mutable notes: It has a nuclear location sequence (NLS) and its codons are optimized for plants.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742004_sequence/1

BBa_K1742012
type: ComponentDefinition
persistent identity: BBa K1742012
display id: BBa_K1742012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S
creator: M??nica Victoria Guti??rrez Salazar
created: 2015-09-06T11:00:00Z
modified: 2015-12-16T01:55:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Translational unit composed by the promoter 35S, the T35s terminator, the CDS Dronpa(145K), Dronpa(145N), the binding domain Gal4 and VP16. The CDS works as a toggle switch activated with green light and deactivated with blue light. Dronpa(145K) changes its conformation with light, being able to dimerize with Dronpa(145N), acting as a transcription factor.
mutable notes: Do a PCR to obtain the Dronpa(145N)
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K105001
component: BBa_K1467101
component: BBa_K1742002
component: BBa_K105007
component: BBa_K1742003
sequence annotation: BBa_K105007
sequence annotation: BBa_K1467101
sequence annotation: BBa_K105001
sequence annotation: BBa_K1742002
sequence annotation: BBa_K1742003
sequence: https://synbiohub.org/public/igem/BBa_K1742012_sequence/1

BBa_K105001
type: Component
persistent identity: component2445634
display id: component2445634
version: 1
top level: BBa_K1742012
access: public
definition: https://synbiohub.org/public/igem/BBa_K105001/1

BBa_K1467101
type: Component
persistent identity: component2445630
display id: component2445630
version: 1
top level: BBa_K1742012
access: public
definition: BBa_K1467101

BBa_K1742002
type: Component
persistent identity: component2445632
display id: component2445632
version: 1
top level: BBa_K1742012
access: public
definition: BBa_K1742002

BBa_K105007
type: Component
persistent identity: component2445631
display id: component2445631
version: 1
top level: BBa_K1742012
access: public
definition: https://synbiohub.org/public/igem/BBa_K105007/1

BBa_K1742003
type: Component
persistent identity: component2445633
display id: component2445633
version: 1
top level: BBa_K1742012
access: public
definition: BBa_K1742003

BBa_K105007
type: SequenceAnnotation
persistent identity: annotation2445631
display id: annotation2445631
version: 1
top level: BBa_K1742012
location: https://synbiohub.org/public/igem/BBa_K1742012/annotation2445631/range2445631/1
component: BBa_K105007

https://synbiohub.org/public/igem/BBa_K1742012/annotation2445631/range2445631/1
type: Range
persistent identity: range2445631
display id: range2445631
version: 1
direction: direction/reverse
top level: BBa_K1742012
start: 1441
end: 1878
orientation: inline

BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2445630
display id: annotation2445630
version: 1
top level: BBa_K1742012
location: https://synbiohub.org/public/igem/BBa_K1742012/annotation2445630/range2445630/1
component: BBa_K1467101

https://synbiohub.org/public/igem/BBa_K1742012/annotation2445630/range2445630/1
type: Range
persistent identity: range2445630
display id: range2445630
version: 1
direction: direction/reverse
top level: BBa_K1742012
start: 1
end: 1432
orientation: inline

BBa_K105001
type: SequenceAnnotation
persistent identity: annotation2445634
display id: annotation2445634
version: 1
top level: BBa_K1742012
location: https://synbiohub.org/public/igem/BBa_K1742012/annotation2445634/range2445634/1
component: BBa_K105001

https://synbiohub.org/public/igem/BBa_K1742012/annotation2445634/range2445634/1
type: Range
persistent identity: range2445634
display id: range2445634
version: 1
direction: direction/reverse
top level: BBa_K1742012
start: 3335
end: 3610
orientation: inline

BBa_K1742002
type: SequenceAnnotation
persistent identity: annotation2445632
display id: annotation2445632
version: 1
top level: BBa_K1742012
location: https://synbiohub.org/public/igem/BBa_K1742012/annotation2445632/range2445632/1
component: BBa_K1742002

https://synbiohub.org/public/igem/BBa_K1742012/annotation2445632/range2445632/1
type: Range
persistent identity: range2445632
display id: range2445632
version: 1
direction: direction/reverse
top level: BBa_K1742012
start: 1885
end: 2577
orientation: inline

BBa_K1742003
type: SequenceAnnotation
persistent identity: annotation2445633
display id: annotation2445633
version: 1
top level: BBa_K1742012
location: https://synbiohub.org/public/igem/BBa_K1742012/annotation2445633/range2445633/1
component: BBa_K1742003

https://synbiohub.org/public/igem/BBa_K1742012/annotation2445633/range2445633/1
type: Range
persistent identity: range2445633
display id: range2445633
version: 1
direction: direction/reverse
top level: BBa_K1742012
start: 2586
end: 3326
orientation: inline

BBa_K1742010
type: ComponentDefinition
persistent identity: BBa K1742010
display id: BBa_K1742010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S:LexABD-DronpaK:T35S - 35S:DronpaN-VP16:T35S
creator: Pilar Baldominos
created: 2015-09-06T11:00:00Z
modified: 2015-12-16T01:54:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26679
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Composite composed of the two transcriptional units needed to obtain a toggle switch control by violet and cian light.
mutable notes: Hola
mutable provenance: It has been synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1742003
component: BBa_K1537015
component: BBa_K1742002
sequence annotation: BBa_K1742003
sequence annotation: BBa_K1537015
sequence annotation: BBa_K1742002
sequence: https://synbiohub.org/public/igem/BBa_K1742010_sequence/1

BBa_K1742003
type: Component
persistent identity: component2445524
display id: component2445524
version: 1
top level: BBa_K1742010
access: public
definition: BBa_K1742003

BBa_K1537015
type: Component
persistent identity: component2445528
display id: component2445528
version: 1
top level: BBa_K1742010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1742002
type: Component
persistent identity: component2445529
display id: component2445529
version: 1
top level: BBa_K1742010
access: public
definition: BBa_K1742002

BBa_K1537015
type: Component
persistent identity: component2445523
display id: component2445523
version: 1
top level: BBa_K1742010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1742003
type: SequenceAnnotation
persistent identity: annotation2445524
display id: annotation2445524
version: 1
top level: BBa_K1742010
location: https://synbiohub.org/public/igem/BBa_K1742010/annotation2445524/range2445524/1
component: BBa_K1742003

https://synbiohub.org/public/igem/BBa_K1742010/annotation2445524/range2445524/1
type: Range
persistent identity: range2445524
display id: range2445524
version: 1
direction: direction/reverse
top level: BBa_K1742010
start: 869
end: 1609
orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2445523
display id: annotation2445523
version: 1
top level: BBa_K1742010
location: https://synbiohub.org/public/igem/BBa_K1742010/annotation2445523/range2445523/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K1742010/annotation2445523/range2445523/1
type: Range
persistent identity: range2445523
display id: range2445523
version: 1
direction: direction/reverse
top level: BBa_K1742010
start: 1
end: 860
orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2445528
display id: annotation2445528
version: 1
top level: BBa_K1742010
location: https://synbiohub.org/public/igem/BBa_K1742010/annotation2445528/range2445528/1
component: BBa_K1537015

https://synbiohub.org/public/igem/BBa_K1742010/annotation2445528/range2445528/1
type: Range
persistent identity: range2445528
display id: range2445528
version: 1
direction: direction/reverse
top level: BBa_K1742010
start: 1618
end: 2477
orientation: inline

BBa_K1742002
type: SequenceAnnotation
persistent identity: annotation2445529
display id: annotation2445529
version: 1
top level: BBa_K1742010
location: https://synbiohub.org/public/igem/BBa_K1742010/annotation2445529/range2445529/1
component: BBa_K1742002

https://synbiohub.org/public/igem/BBa_K1742010/annotation2445529/range2445529/1
type: Range
persistent identity: range2445529
display id: range2445529
version: 1
direction: direction/reverse
top level: BBa_K1742010
start: 2484
end: 3176
orientation: inline

BBa_K1554003
type: ComponentDefinition
persistent identity: BBa K1554003
display id: BBa_K1554003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EaDAcT
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:29:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: diacylglycerol acetyltransferase
mutable notes: BioBrick compatible
mutable provenance: It comes from Euonymus alatus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554003_sequence/1

BBa_K1467103
type: ComponentDefinition
persistent identity: BBa K1467103
display id: BBa_K1467103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible PDF1.2 promotor
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:37:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PDF1.2 is a plant specific promoter and 5??? untranslated region which is induced by methyl jasmonate. The part is compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467103_sequence/1

BBa_K1742011
type: ComponentDefinition
persistent identity: BBa K1742011
display id: BBa_K1742011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35S:LacIBDBD-DronpaK:T35S - 35S:DronpaN-VP16:T35S
creator: M??nica Victoria Guti??rrez Salazar
created: 2015-09-06T11:00:00Z
modified: 2015-12-16T01:54:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Translational unit composed by the promoter 35S, the T35s terminator, and the CDS Dronpa(145K) and Dronpa(145N). The CDS works as a toggle switch activated with green light and deactivated with blue light. Dronpa(145K) changes its conformation with light, being able to dimerize with Dronpa(145N), acting as a transcription factor.
mutable notes: Change the aminoacid.
mutable provenance: Dronpa(145K) is synthetized. Dronpa(145N) was obtained by PCR, changing 145K by 145N.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537015
component: BBa_K1742002
component: BBa_K1742003
component: BBa_K105001
sequence annotation: BBa_K1742003
sequence annotation: BBa_K1742002
sequence annotation: BBa_K1537015
sequence annotation: BBa_K105001
sequence: https://synbiohub.org/public/igem/BBa_K1742011_sequence/1

BBa_K1537015
type: Component
persistent identity: component2445407
display id: component2445407
version: 1
top level: BBa_K1742011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1742002
type: Component
persistent identity: component2445409
display id: component2445409
version: 1
top level: BBa_K1742011
access: public
definition: BBa_K1742002

BBa_K1742003
type: Component
persistent identity: component2445408
display id: component2445408
version: 1
top level: BBa_K1742011
access: public
definition: BBa_K1742003

BBa_K105001
type: Component
persistent identity: component2445410
display id: component2445410
version: 1
top level: BBa_K1742011
access: public
definition: https://synbiohub.org/public/igem/BBa_K105001/1

BBa_K1742003
type: SequenceAnnotation
persistent identity: annotation2445408
display id: annotation2445408
version: 1
top level: BBa_K1742011
location: https://synbiohub.org/public/igem/BBa_K1742011/annotation2445408/range2445408/1
component: BBa_K1742003

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display id: range2445408
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BBa_K1742002
type: SequenceAnnotation
persistent identity: annotation2445409
display id: annotation2445409
version: 1
top level: BBa_K1742011
location: https://synbiohub.org/public/igem/BBa_K1742011/annotation2445409/range2445409/1
component: BBa_K1742002

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persistent identity: range2445409
display id: range2445409
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BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2445407
display id: annotation2445407
version: 1
top level: BBa_K1742011
location: https://synbiohub.org/public/igem/BBa_K1742011/annotation2445407/range2445407/1
component: BBa_K1537015

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persistent identity: range2445407
display id: range2445407
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BBa_K105001
type: SequenceAnnotation
persistent identity: annotation2445410
display id: annotation2445410
version: 1
top level: BBa_K1742011
location: https://synbiohub.org/public/igem/BBa_K1742011/annotation2445410/range2445410/1
component: BBa_K105001

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persistent identity: range2445410
display id: range2445410
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BBa_K1618035
type: ComponentDefinition
persistent identity: BBa K1618035
display id: BBa_K1618035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Acyltransferase Candidate 3 Expression Construct
creator: Leda Coelewij
created: 2015-09-08T11:00:00Z
modified: 2015-12-16T02:57:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2035_
m user id: 4206
owner id: 24808
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Acylates Starch
mutable notes: None
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1467101
component: BBa_K1618028
component: BBa_K1618037
component: BBa_K1618003
sequence annotation: BBa_K1618028
sequence annotation: BBa_K1618003
sequence annotation: BBa_K1618037
sequence annotation: BBa_K1467101
sequence: https://synbiohub.org/public/igem/BBa_K1618035_sequence/1

BBa_K1467101
type: Component
persistent identity: component2462160
display id: component2462160
version: 1
top level: BBa_K1618035
access: public
definition: BBa_K1467101

BBa_K1618028
type: Component
persistent identity: component2462161
display id: component2462161
version: 1
top level: BBa_K1618035
access: public
definition: BBa_K1618028

BBa_K1618037
type: Component
persistent identity: component2462163
display id: component2462163
version: 1
top level: BBa_K1618035
access: public
definition: BBa_K1618037

BBa_K1618003
type: Component
persistent identity: component2462162
display id: component2462162
version: 1
top level: BBa_K1618035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1618003/1

BBa_K1618028
type: SequenceAnnotation
persistent identity: annotation2462161
display id: annotation2462161
version: 1
top level: BBa_K1618035
location: https://synbiohub.org/public/igem/BBa_K1618035/annotation2462161/range2462161/1
component: BBa_K1618028

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type: Range
persistent identity: range2462161
display id: range2462161
version: 1
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start: 1439
end: 1615
orientation: inline

BBa_K1618003
type: SequenceAnnotation
persistent identity: annotation2462162
display id: annotation2462162
version: 1
top level: BBa_K1618035
location: https://synbiohub.org/public/igem/BBa_K1618035/annotation2462162/range2462162/1
component: BBa_K1618003

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type: Range
persistent identity: range2462162
display id: range2462162
version: 1
direction: direction/reverse
top level: BBa_K1618035
start: 1622
end: 2527
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BBa_K1618037
type: SequenceAnnotation
persistent identity: annotation2462163
display id: annotation2462163
version: 1
top level: BBa_K1618035
location: https://synbiohub.org/public/igem/BBa_K1618035/annotation2462163/range2462163/1
component: BBa_K1618037

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persistent identity: range2462163
display id: range2462163
version: 1
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start: 2536
end: 2747
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BBa_K1467101
type: SequenceAnnotation
persistent identity: annotation2462160
display id: annotation2462160
version: 1
top level: BBa_K1618035
location: https://synbiohub.org/public/igem/BBa_K1618035/annotation2462160/range2462160/1
component: BBa_K1467101

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type: Range
persistent identity: range2462160
display id: range2462160
version: 1
direction: direction/reverse
top level: BBa_K1618035
start: 1
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BBa_K2017010
type: ComponentDefinition
persistent identity: BBa K2017010
display id: BBa_K2017010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 35s + Ga20ox consense + RSIAT-TEV-Luciferase + Tnos
creator: Monica Victoria Gutierrez Salazar
created: 2016-10-03T11:00:00Z
modified: 2016-10-22T05:13:20Z
discontinued: false
dominant: false
group u list: _2484_
m user id: 26626
owner id: 26626
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Modul of the gRNA testing system created by Valencia UPV 2016 team. This system aims to test the functionality and efficiency of a chosen gRNA to target a particular gene. This system is modular, as the gene inserted can be chosen by the user, only taking into account the required overhangs (5'-AATG-3' and 5'-TTCG-3'). It includes a promoter 35s (BBa_K1537015), 20 nucleotides of the Ga20ox consensus gene of Oryza sativa (rice), the reporter luciferase with RSIAT+TEV linker and the terminator Tnos. Luciferase is out of its reading frame due to a nucleotide added upstream to the linker, and the initial ATG has been removed. This means that when the luciferase is translated, it will not be functional. To make it functional, it is necessary to make indels in the Ga20ox fragment that put the luciferase in the correct reading frame. These indels can be made using the CRISPR/Cas9 system, which is the aim of this device.
mutable notes: It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'.
mutable provenance: This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537015
component: BBa_K1484215
component: BBa_K2017006
sequence annotation: BBa_K2017006
sequence annotation: BBa_K1537015
sequence annotation: BBa_K1484215
sequence: https://synbiohub.org/public/igem/BBa_K2017010_sequence/1

BBa_K1537015
type: Component
persistent identity: component2487426
display id: component2487426
version: 1
top level: BBa_K2017010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1537015/1

BBa_K1484215
type: Component
persistent identity: component2487433
display id: component2487433
version: 1
top level: BBa_K2017010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1484215/1

BBa_K2017006
type: Component
persistent identity: component2487430
display id: component2487430
version: 1
top level: BBa_K2017010
access: public
definition: https://synbiohub.org/public/igem/BBa_K2017006/1

BBa_K2017006
type: SequenceAnnotation
persistent identity: annotation2487430
display id: annotation2487430
version: 1
top level: BBa_K2017010
location: https://synbiohub.org/public/igem/BBa_K2017010/annotation2487430/range2487430/1
component: BBa_K2017006

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display id: range2487430
version: 1
direction: direction/reverse
top level: BBa_K2017010
start: 869
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orientation: inline

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2487426
display id: annotation2487426
version: 1
top level: BBa_K2017010
location: https://synbiohub.org/public/igem/BBa_K2017010/annotation2487426/range2487426/1
component: BBa_K1537015

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type: Range
persistent identity: range2487426
display id: range2487426
version: 1
direction: direction/reverse
top level: BBa_K2017010
start: 1
end: 860
orientation: inline

BBa_K1484215
type: SequenceAnnotation
persistent identity: annotation2487433
display id: annotation2487433
version: 1
top level: BBa_K2017010
location: https://synbiohub.org/public/igem/BBa_K2017010/annotation2487433/range2487433/1
component: BBa_K1484215

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type: Range
persistent identity: range2487433
display id: range2487433
version: 1
direction: direction/reverse
top level: BBa_K2017010
start: 2577
end: 2869
orientation: inline