//chassis/eukaryote/athaliana
type: Collection
persistent identity: chassis eukaryote athaliana
display id: chassis_eukaryote_athaliana
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K382034
member: BBa_K382021
member: BBa_K1478001
member: BBa_K382026
member: BBa_K382017
member: BBa_K382000
member: BBa_K382004
member: BBa_K382027
member: BBa_K1478000
member: BBa_K382014
member: BBa_K382022
member: BBa_K382005
member: BBa_K382001
member: BBa_K382018
member: BBa_K382002
member: BBa_K382015
member: BBa_K382028
member: BBa_K382010
member: BBa_K382040
member: BBa_K1982003
member: BBa_K382019
member: BBa_K1982009
member: BBa_K382041
member: BBa_K382011
member: BBa_K382025
member: BBa_K382012
member: BBa_K1982002
member: BBa_K1982010
member: BBa_K1478002
member: BBa_K382020
member: BBa_K382003

BBa_K382014
type: ComponentDefinition
persistent identity: BBa K382014
display id: BBa_K382014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ihpRNA construct against Arabidopsis Bet v 1 allergen
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:16:19Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intron containing hairpin RNA construct for targeting knockdown of the Bet v 1 allergen homolog in Arabidopsis. Assembled with PDK intron from pHANNIBAL.
mutable provenance: Intermediate parts PCRed from Arabidopsis genomic DNA and pHANNIBAL vector. Assembled using Silver Standard 21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382014_sequence/1

BBa_K382034
type: ComponentDefinition
persistent identity: BBa K382034
display id: BBa_K382034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Act2pLacO (Actin promoter + 2x LacO)
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:09:19Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A constitutive plant actin promoter followed by two copies of the Lac operator. This allows control of promoter activity by LacI.
mutable notes: Conforms to biobrick standard 21
mutable provenance: synthesized by mr.gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K382034_sequence/1

BBa_K382004
type: ComponentDefinition
persistent identity: BBa K382004
display id: BBa_K382004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:58Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has a constitutive plant promoter and in-frame Gus reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Standard 21.
mutable provenance: Modified vector pORE_R from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382004_sequence/1

BBa_K382012
type: ComponentDefinition
persistent identity: BBa K382012
display id: BBa_K382012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amiRNA Construct for lycopene beta cyclase (LYC)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:16:32Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plant artificial microRNA construct for targeted knockdown of LYC.
mutable notes: MicroRNA targeting sequences designed with the Web MicroRNA Designer: http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Designer;project=stdwmd
mutable provenance: Modified RS300 plasmid: http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Home;project=stdwmd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382012_sequence/1

BBa_K382021
type: ComponentDefinition
persistent identity: BBa K382021
display id: BBa_K382021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Miraculin
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-05-27T01:42:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Miraculin is a 'flavor inverting' protein, found naturally in the fruit of the plant fruit of Synsepalum dulcificum. Not sweet by itself, miraculin binds to taste receptors on the tongue, possibly altering the structure of the receptors and causing traditionally 'sour' flavors to be received as 'sweet'.
mutable notes: N/A
mutable provenance: Synthesized by Mr. Gene, optimized for expression in Arabidopsis. Derived from Genbank sequence BAH84844
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: open reading fram (no stop codon)
sequence: https://synbiohub.org/public/igem/BBa_K382021_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2113978
display id: annotation2113978
version: 1
top level: BBa_K382021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382021/annotation2113978/range2113978/1

https://synbiohub.org/public/igem/BBa_K382021/annotation2113978/range2113978/1
type: Range
persistent identity: range2113978
display id: range2113978
version: 1
direction: direction/forward
top level: BBa_K382021
start: 1
end: 3
orientation: inline

open reading fram (no stop codon)
type: SequenceAnnotation
persistent identity: annotation2113979
display id: annotation2113979
version: 1
top level: BBa_K382021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382021/annotation2113979/range2113979/1

https://synbiohub.org/public/igem/BBa_K382021/annotation2113979/range2113979/1
type: Range
persistent identity: range2113979
display id: range2113979
version: 1
direction: direction/forward
top level: BBa_K382021
start: 1
end: 660
orientation: inline

BBa_K1982010
type: ComponentDefinition
persistent identity: BBa K1982010
display id: BBa_K1982010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRY2-VP64(Eukaryotic LACE system)
creator: Zexu Li
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T10:53:31Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" ! colspan="2" style="background:#FFBF00;"|RBS-CRY2-VP64-HA-FLAG |- |'''Function''' |gene activation |- |'''Use in''' |Prokaryotic cells |- |'''RFC standard''' |[http://parts.igem.org/Help:Assembly_standard_10 RFC 10] |- |'''Backbone''' |pSB1C3 |- |'''Submitted by''' |[http://2016.igem.org/Team:NEU-China NEU-China 2016] |} The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a VP64 fused to its C terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription. We used the full-length CRY2 (BBa_K1982002) and fused it to the transcription activator domain VP64 (BBa_K1982000). An prokaryotic RBS sequence from the Community collection(BBa_B0034) fused to the beginning of CRY2-VP64. For detection of expression the fusion protein was tagged with a FLAG-epitope coding sequence (gactacaaggacgacgacgacaaa) .Figure 1 illustrates the detailed design of the whole device. <!-- Add more about the biology of this part here ===Usage and Biology=== Protein data table for BioBrick <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_ ">BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0 Nucleotide sequence in RFC 10: (underlined part encodes the protein) ATGAAGATG ... GACGACAAATAATAA ORF from nucleotide position 1 to 2076 (excluding stop-codon) Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below) 1 101 201 301 401 501 601 MKMDKKTIVWFRRDL…
mutable notes: no
mutable provenance: CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: SV40 NLS
sequence annotation: VP16
sequence annotation: HA
sequence annotation: TAA
sequence annotation: CRY2
sequence annotation: Eukaryotic rbs
sequence annotation: Linker
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1982010_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2517281
display id: annotation2517281
version: 1
top level: BBa_K1982010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517281/range2517281/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517281/range2517281/1
type: Range
persistent identity: range2517281
display id: range2517281
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 19
end: 21
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517283
display id: annotation2517283
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517283/range2517283/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517283/range2517283/1
type: Range
persistent identity: range2517283
display id: range2517283
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1855
end: 1875
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517285
display id: annotation2517285
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517285/range2517285/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517285/range2517285/1
type: Range
persistent identity: range2517285
display id: range2517285
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1915
end: 1953
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517289
display id: annotation2517289
version: 1
top level: BBa_K1982010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517289/range2517289/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517289/range2517289/1
type: Range
persistent identity: range2517289
display id: range2517289
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2037
end: 2063
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517284
display id: annotation2517284
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517284/range2517284/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517284/range2517284/1
type: Range
persistent identity: range2517284
display id: range2517284
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1876
end: 1914
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2517291
display id: annotation2517291
version: 1
top level: BBa_K1982010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517291/range2517291/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517291/range2517291/1
type: Range
persistent identity: range2517291
display id: range2517291
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2095
end: 2100
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517286
display id: annotation2517286
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517286/range2517286/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517286/range2517286/1
type: Range
persistent identity: range2517286
display id: range2517286
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1954
end: 1992
orientation: inline

CRY2
type: SequenceAnnotation
persistent identity: annotation2517282
display id: annotation2517282
version: 1
top level: BBa_K1982010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517282/range2517282/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517282/range2517282/1
type: Range
persistent identity: range2517282
display id: range2517282
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 22
end: 1854
orientation: inline

Eukaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2517280
display id: annotation2517280
version: 1
top level: BBa_K1982010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517280/range2517280/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517280/range2517280/1
type: Range
persistent identity: range2517280
display id: range2517280
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1
end: 18
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517288
display id: annotation2517288
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517288/range2517288/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517288/range2517288/1
type: Range
persistent identity: range2517288
display id: range2517288
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2032
end: 2037
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517287
display id: annotation2517287
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517287/range2517287/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517287/range2517287/1
type: Range
persistent identity: range2517287
display id: range2517287
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1993
end: 2031
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517290
display id: annotation2517290
version: 1
top level: BBa_K1982010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517290/range2517290/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517290/range2517290/1
type: Range
persistent identity: range2517290
display id: range2517290
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2071
end: 2094
orientation: inline

BBa_K382028
type: ComponentDefinition
persistent identity: BBa K382028
display id: BBa_K382028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Brazzein with StrepII C-terminus
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:10:47Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Brazzein (BBa_K382020) with a StrepII tag for western blot analysis.
mutable notes: N/A
mutable provenance: BBa_K382020 with biobricked strepII tag obtained from the Silver Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: strepII-tag
sequence annotation: Arabidopsis optimized Brazzein
sequence annotation: biobrick scar (standard 23)
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K382028_sequence/1

strepII-tag
type: SequenceAnnotation
persistent identity: annotation2114282
display id: annotation2114282
version: 1
top level: BBa_K382028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382028/annotation2114282/range2114282/1

https://synbiohub.org/public/igem/BBa_K382028/annotation2114282/range2114282/1
type: Range
persistent identity: range2114282
display id: range2114282
version: 1
direction: direction/forward
top level: BBa_K382028
start: 172
end: 201
orientation: inline

Arabidopsis optimized Brazzein
type: SequenceAnnotation
persistent identity: annotation2114280
display id: annotation2114280
version: 1
top level: BBa_K382028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382028/annotation2114280/range2114280/1

https://synbiohub.org/public/igem/BBa_K382028/annotation2114280/range2114280/1
type: Range
persistent identity: range2114280
display id: range2114280
version: 1
direction: direction/forward
top level: BBa_K382028
start: 1
end: 165
orientation: inline

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114281
display id: annotation2114281
version: 1
top level: BBa_K382028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382028/annotation2114281/range2114281/1

https://synbiohub.org/public/igem/BBa_K382028/annotation2114281/range2114281/1
type: Range
persistent identity: range2114281
display id: range2114281
version: 1
direction: direction/forward
top level: BBa_K382028
start: 166
end: 171
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2114279
display id: annotation2114279
version: 1
top level: BBa_K382028
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382028/annotation2114279/range2114279/1

https://synbiohub.org/public/igem/BBa_K382028/annotation2114279/range2114279/1
type: Range
persistent identity: range2114279
display id: range2114279
version: 1
direction: direction/forward
top level: BBa_K382028
start: 1
end: 3
orientation: inline

BBa_K1478000
type: ComponentDefinition
persistent identity: BBa K1478000
display id: BBa_K1478000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expa4 plant secretion signal, localizes to extracellular space
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-05-27T02:12:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for first 20 aminoacids of plant protein Exansin4. Secretion signal for plants (derived from A. thaliana). When fused to a protein, directs the protein to extracellular space.
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Derived from Expansin4 from Arabidopsis thaliana(NP_181500).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478000_sequence/1

BBa_K382041
type: ComponentDefinition
persistent identity: BBa K382041
display id: BBa_K382041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Brazzein+2xYFP
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:07:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Brazzein with a 2xYFP tag.
mutable notes: Conforms to Silver standard 21
mutable provenance: Brazzein was synthesized. YFP tag was added by cloning
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 2xYFP
sequence annotation: start codon
sequence annotation: biobrick scar (standard 23)
sequence annotation: Arabidopsis optimized Brazzein
sequence: https://synbiohub.org/public/igem/BBa_K382041_sequence/1

2xYFP
type: SequenceAnnotation
persistent identity: annotation2114278
display id: annotation2114278
version: 1
top level: BBa_K382041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114278/range2114278/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114278/range2114278/1
type: Range
persistent identity: range2114278
display id: range2114278
version: 1
direction: direction/forward
top level: BBa_K382041
start: 172
end: 1665
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2114275
display id: annotation2114275
version: 1
top level: BBa_K382041
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114275/range2114275/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114275/range2114275/1
type: Range
persistent identity: range2114275
display id: range2114275
version: 1
direction: direction/forward
top level: BBa_K382041
start: 1
end: 3
orientation: inline

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114277
display id: annotation2114277
version: 1
top level: BBa_K382041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114277/range2114277/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114277/range2114277/1
type: Range
persistent identity: range2114277
display id: range2114277
version: 1
direction: direction/forward
top level: BBa_K382041
start: 166
end: 171
orientation: inline

Arabidopsis optimized Brazzein
type: SequenceAnnotation
persistent identity: annotation2114276
display id: annotation2114276
version: 1
top level: BBa_K382041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382041/annotation2114276/range2114276/1

https://synbiohub.org/public/igem/BBa_K382041/annotation2114276/range2114276/1
type: Range
persistent identity: range2114276
display id: range2114276
version: 1
direction: direction/forward
top level: BBa_K382041
start: 1
end: 165
orientation: inline

BBa_K382003
type: ComponentDefinition
persistent identity: BBa K382003
display id: BBa_K382003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Expression Series Vector with BioBrick MCS (Kan reistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-05-27T01:46:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive promoter upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O4 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K382003_sequence/1

BBa_K382027
type: ComponentDefinition
persistent identity: BBa K382027
display id: BBa_K382027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Miraculin with StrepII C-terminus tag
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:11:10Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Miraculin (BBa_K382021) with a StrepII tag for western blot analysis.
mutable notes: N/A
mutable provenance: BBa_K382021 with biobricked strepII tag obtained from the Silver Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K382027_sequence/1

BBa_K382018
type: ComponentDefinition
persistent identity: BBa K382018
display id: BBa_K382018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PDK intron
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:17:32Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PDK intron from pHANNIBAL vector for use in plant intron containing hairpin RNAi construction.
mutable provenance: pHANNIBAL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence: https://synbiohub.org/public/igem/BBa_K382018_sequence/1

BBa_K1982002
type: ComponentDefinition
persistent identity: BBa K1982002
display id: BBa_K1982002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
creator: Zexu Li
created: 2016-09-10T11:00:00Z
modified: 2016-10-09T12:15:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
mutable notes: mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: Prokaryotic rbs
sequence: https://synbiohub.org/public/igem/BBa_K1982002_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2483021
display id: annotation2483021
version: 1
top level: BBa_K1982002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982002/annotation2483021/range2483021/1

https://synbiohub.org/public/igem/BBa_K1982002/annotation2483021/range2483021/1
type: Range
persistent identity: range2483021
display id: range2483021
version: 1
direction: direction/forward
top level: BBa_K1982002
start: 19
end: 21
orientation: inline

Prokaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2488670
display id: annotation2488670
version: 1
top level: BBa_K1982002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982002/annotation2488670/range2488670/1

https://synbiohub.org/public/igem/BBa_K1982002/annotation2488670/range2488670/1
type: Range
persistent identity: range2488670
display id: range2488670
version: 1
direction: direction/forward
top level: BBa_K1982002
start: 1
end: 18
orientation: inline

BBa_K382019
type: ComponentDefinition
persistent identity: BBa K382019
display id: BBa_K382019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ihpRNA construct against Arabidopsis Ger3 allergen
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:14:54Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intron containing hairpin RNAi construct targeting Arabidopsis Germin 3 allergen sequence. Contains PDK intron from pHANNIBAL, assembled using Silver standard 21.
mutable provenance: pHANNIBAL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382019_sequence/1

BBa_K382015
type: ComponentDefinition
persistent identity: BBa K382015
display id: BBa_K382015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ihpRNA construct against Arabidopsis LTP Allergen
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:15:59Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intron containing hairpin RNA construct for RNAi knocktown of Lipid Transfer Protein allergen in Arabidopsis. Intron is PDK intron from pHANNIBAL assembled with 300bp of sense and antisense DNA from Arabidopsis genomic DNA assembled with Silver standard 21
mutable provenance: Arabidopsis genomic DNA, pHANNIBAL vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382015_sequence/1

BBa_K1982009
type: ComponentDefinition
persistent identity: BBa K1982009
display id: BBa_K1982009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
creator: Zexu Li
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T12:15:06Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
mutable notes: mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Eukaryotic rbs
sequence annotation: atg
sequence: https://synbiohub.org/public/igem/BBa_K1982009_sequence/1

Eukaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2488673
display id: annotation2488673
version: 1
top level: BBa_K1982009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982009/annotation2488673/range2488673/1

https://synbiohub.org/public/igem/BBa_K1982009/annotation2488673/range2488673/1
type: Range
persistent identity: range2488673
display id: range2488673
version: 1
direction: direction/forward
top level: BBa_K1982009
start: 1
end: 18
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2488672
display id: annotation2488672
version: 1
top level: BBa_K1982009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982009/annotation2488672/range2488672/1

https://synbiohub.org/public/igem/BBa_K1982009/annotation2488672/range2488672/1
type: Range
persistent identity: range2488672
display id: range2488672
version: 1
direction: direction/forward
top level: BBa_K1982009
start: 19
end: 21
orientation: inline

BBa_K382011
type: ComponentDefinition
persistent identity: BBa K382011
display id: BBa_K382011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amiRNA Construct for carotene beta-ring hydroxylase (BETA-OHASE 1)
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:16:44Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: plant artificial microRNA construct for targeted knock-down of carotene beta-ring hydroxylase (BETA-OHASE 1). Needs to be transferred to a plant expression vector.
mutable notes: designed with WebMicroRNA designer http://wmd3.weigelworld.org/cgi-bin/webapp.cgi
mutable provenance: modified from RS300, target sequences were synthesized as oligos,
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382011_sequence/1

BBa_K382000
type: ComponentDefinition
persistent identity: BBa K382000
display id: BBa_K382000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Open Series Vector with BioBrick MCS (Basta resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:19:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Basta resistance in plants. Has no promoter or reporter.
mutable notes: Conforms to Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O1 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K382000_sequence/1

BBa_K382002
type: ComponentDefinition
persistent identity: BBa K382002
display id: BBa_K382002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Expression Series Vector with BioBrick MCS, Basta resistance
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-05-27T01:45:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the pENTCUP promoter upstream of MCS for constitutive expression of gene of interest.
mutable notes: Silver standard 21
mutable provenance: Modified from pORE_E3 found in Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence: https://synbiohub.org/public/igem/BBa_K382002_sequence/1

BBa_K382005
type: ComponentDefinition
persistent identity: BBa K382005
display id: BBa_K382005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE GFP Reporter Series Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:18:36Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has constitutive plant promoter and in-frame GFP reporter. Conforms to Silver BIoBrick Assembly Standard 21.
mutable notes: Silver Standard 21
mutable provenance: Modified vector pORE_R2 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382005_sequence/1

BBa_K382017
type: ComponentDefinition
persistent identity: BBa K382017
display id: BBa_K382017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ihpRNA construct against Arabidopsis LTP Allergen
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:15:34Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intron containing hairpin RNAi construct targeting Arabidopsis Lipid Transfer Protein allergen. PDK intron from pHANNIBAL, 300 bp of LTP sense and antisense from Arabidopsis genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382017_sequence/1

BBa_K382001
type: ComponentDefinition
persistent identity: BBa K382001
display id: BBa_K382001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pORE Open Serices Vector with BioBrick MCS (Kan resistance)
creator: Christina Agapakis
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:19:10Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 2601
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has no promoter or reporter. Conforms to Silver BioBrick Assembly Standard 21.
mutable notes: Silver Assembly Standard 21
mutable provenance: Modified vector pORE_O1 from the Arabidopsis Information Resource
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K382001_sequence/1

BBa_K382040
type: ComponentDefinition
persistent identity: BBa K382040
display id: BBa_K382040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Miraculin+2xYFP
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:06:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Miraculin with a 2xYFP tag.
mutable notes: Conforms to Silver standard 21
mutable provenance: Miraculin was synthesized. YFP tag was added by cloning
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Arabidopsis optimized Miraculin
sequence annotation: 2xYFP
sequence annotation: biobrick scar (standard 23)
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K382040_sequence/1

Arabidopsis optimized Miraculin
type: SequenceAnnotation
persistent identity: annotation2114287
display id: annotation2114287
version: 1
top level: BBa_K382040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114287/range2114287/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114287/range2114287/1
type: Range
persistent identity: range2114287
display id: range2114287
version: 1
direction: direction/forward
top level: BBa_K382040
start: 1
end: 660
orientation: inline

2xYFP
type: SequenceAnnotation
persistent identity: annotation2114289
display id: annotation2114289
version: 1
top level: BBa_K382040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114289/range2114289/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114289/range2114289/1
type: Range
persistent identity: range2114289
display id: range2114289
version: 1
direction: direction/forward
top level: BBa_K382040
start: 667
end: 2160
orientation: inline

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114288
display id: annotation2114288
version: 1
top level: BBa_K382040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114288/range2114288/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114288/range2114288/1
type: Range
persistent identity: range2114288
display id: range2114288
version: 1
direction: direction/forward
top level: BBa_K382040
start: 661
end: 666
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2114286
display id: annotation2114286
version: 1
top level: BBa_K382040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382040/annotation2114286/range2114286/1

https://synbiohub.org/public/igem/BBa_K382040/annotation2114286/range2114286/1
type: Range
persistent identity: range2114286
display id: range2114286
version: 1
direction: direction/forward
top level: BBa_K382040
start: 1
end: 3
orientation: inline

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K382026
type: ComponentDefinition
persistent identity: BBa K382026
display id: BBa_K382026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Brazzein with StrepII N-terminus
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:11:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Brazzein (BBa_K382020) with a StrepII tag for western blot analysis.
mutable notes: N/A
mutable provenance: BBa_K382020 with biobricked strepII tag obtained from the Silver Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: biobrick scar (standard 23)
sequence annotation: Arabidopsis optimized Brazzein
sequence annotation: strepII-tag
sequence: https://synbiohub.org/public/igem/BBa_K382026_sequence/1

biobrick scar (standard 23)
type: SequenceAnnotation
persistent identity: annotation2114283
display id: annotation2114283
version: 1
top level: BBa_K382026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K382026/annotation2114283/range2114283/1

https://synbiohub.org/public/igem/BBa_K382026/annotation2114283/range2114283/1
type: Range
persistent identity: range2114283
display id: range2114283
version: 1
direction: direction/forward
top level: BBa_K382026
start: 31
end: 36
orientation: inline

Arabidopsis optimized Brazzein
type: SequenceAnnotation
persistent identity: annotation2114285
display id: annotation2114285
version: 1
top level: BBa_K382026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382026/annotation2114285/range2114285/1

https://synbiohub.org/public/igem/BBa_K382026/annotation2114285/range2114285/1
type: Range
persistent identity: range2114285
display id: range2114285
version: 1
direction: direction/forward
top level: BBa_K382026
start: 37
end: 201
orientation: inline

strepII-tag
type: SequenceAnnotation
persistent identity: annotation2114284
display id: annotation2114284
version: 1
top level: BBa_K382026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382026/annotation2114284/range2114284/1

https://synbiohub.org/public/igem/BBa_K382026/annotation2114284/range2114284/1
type: Range
persistent identity: range2114284
display id: range2114284
version: 1
direction: direction/forward
top level: BBa_K382026
start: 1
end: 30
orientation: inline

BBa_K382020
type: ComponentDefinition
persistent identity: BBa K382020
display id: BBa_K382020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Brazzein
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-05-27T01:42:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Brazzein is a sweet-tasting protein that is found in the Western African fruit, the Oubli (Pentadiplandra brazzeana). The protein consists of a 54 amino acid sequence. It is sweeter than sugar and is used as an alternative low calorie sweetener.
mutable notes: N/A
mutable provenance: Synthesis was performed by Mr. Gene, optimized for Arabidopsis expression. Based on Genbank sequence ACK76425.1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: open reading frame (no stop codon)
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K382020_sequence/1

open reading frame (no stop codon)
type: SequenceAnnotation
persistent identity: annotation2113977
display id: annotation2113977
version: 1
top level: BBa_K382020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K382020/annotation2113977/range2113977/1

https://synbiohub.org/public/igem/BBa_K382020/annotation2113977/range2113977/1
type: Range
persistent identity: range2113977
display id: range2113977
version: 1
direction: direction/forward
top level: BBa_K382020
start: 1
end: 165
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2113975
display id: annotation2113975
version: 1
top level: BBa_K382020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K382020/annotation2113975/range2113975/1

https://synbiohub.org/public/igem/BBa_K382020/annotation2113975/range2113975/1
type: Range
persistent identity: range2113975
display id: range2113975
version: 1
direction: direction/forward
top level: BBa_K382020
start: 1
end: 3
orientation: inline

BBa_K1982003
type: ComponentDefinition
persistent identity: BBa K1982003
display id: BBa_K1982003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CIBN(the N-terminal fragment of CIB1)
creator: Zexu Li
created: 2016-09-11T11:00:00Z
modified: 2016-09-12T05:35:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Encodes the N-terminal fragment of transcription factor CIB1 (cryptochrome-interacting basic-helix-loop-helix). CIB1 interacts with CRY2 (cryptochrome 2) in a blue light-specific manner in yeast and Arabidopsis cells, and it acts together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. CIB1 positively regulates FT expression. Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
mutable notes: no
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K1982003_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2482939
display id: annotation2482939
version: 1
top level: BBa_K1982003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982003/annotation2482939/range2482939/1

https://synbiohub.org/public/igem/BBa_K1982003/annotation2482939/range2482939/1
type: Range
persistent identity: range2482939
display id: range2482939
version: 1
direction: direction/forward
top level: BBa_K1982003
start: 607
end: 612
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2482938
display id: annotation2482938
version: 1
top level: BBa_K1982003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982003/annotation2482938/range2482938/1

https://synbiohub.org/public/igem/BBa_K1982003/annotation2482938/range2482938/1
type: Range
persistent identity: range2482938
display id: range2482938
version: 1
direction: direction/forward
top level: BBa_K1982003
start: 1
end: 3
orientation: inline

BBa_K382025
type: ComponentDefinition
persistent identity: BBa K382025
display id: BBa_K382025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabidopsis optimized Miraculin with StrepII N-terminus tag
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:11:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabidopsis optimized Miraculin (BBa_K382021) with a StrepII tag for western blot analysis.
mutable notes: N/A
mutable provenance: BBa_K382021 with biobricked strepII tag obtained from the Silver Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K382025_sequence/1

BBa_K382010
type: ComponentDefinition
persistent identity: BBa K382010
display id: BBa_K382010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amiRNA Construct for lycopene epsilon cyclase (LUT2)
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:16:55Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Artificial micro RNA targeting the Arabidopsis LUT2 gene.
mutable notes: Designed with the artificial Web MicroRNA Designer (http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Home;project=stdwmd)
mutable provenance: Modified from RS300 to target the Arabidopsis LUT2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K382010_sequence/1

BBa_K1478002
type: ComponentDefinition
persistent identity: BBa K1478002
display id: BBa_K1478002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Top metal binding protein. Binds zinc, arsenic, copper and cadmium.
creator: Fabian Fr??mling
created: 2014-09-17T11:00:00Z
modified: 2015-05-27T02:23:01Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Multiple domain protein. Domains bind zinc, arsenic, copper and cadmium. Sequences derived from metallothioneins and transcription factors of different organisms. Arsenic, zinc and cadmium binding regions are full length metallothioneins. Zinc binding region derived from a transcritpion factor binding domain. Different protein binding regions are separated with glycerin-serine spacers to improve domain folding. Metal binding regions consists of disulfide bonds. For expression in E. coli disulfide bond forming strains are useful (e. g. Origami).
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Arsenic (CAA06729), zinc (CAD88267) and cadmium (AAN06822) metallothioneins are full length. Copper AAA34542)domain is partial.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1478002_sequence/1

BBa_K382022
type: ComponentDefinition
persistent identity: BBa K382022
display id: BBa_K382022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pENTCUP2 plant specific promoter
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:13:46Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pENTCUP2 promoter for the constitutive expression of genes in plants.
mutable notes: Amplified from PCR from the pORE E3 plasmid.
mutable provenance: Derived from the pORE vector series (see http://2010.igem.org/Team:Harvard).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K382022_sequence/1