//chassis/aferrooxidans
type: Collection
persistent identity: chassis aferrooxidans
display id: chassis_aferrooxidans
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J3902/1
member: https://synbiohub.org/public/igem/BBa_K952000/1

//chassis/bacteriophage
type: Collection
persistent identity: chassis bacteriophage
display id: chassis_bacteriophage
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1031011/1
member: https://synbiohub.org/public/igem/BBa_K1781000/1
member: https://synbiohub.org/public/igem/chassis_bacteriophage_t7/1
member: https://synbiohub.org/public/igem/chassis_bacteriophage_t4/1
member: https://synbiohub.org/public/igem/chassis_bacteriophage_t3/1

//chassis/eukaryote
type: Collection
persistent identity: chassis eukaryote
display id: chassis_eukaryote
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K678042/1
member: https://synbiohub.org/public/igem/BBa_J05218/1
member: https://synbiohub.org/public/igem/BBa_I712004/1
member: https://synbiohub.org/public/igem/BBa_K678068/1
member: https://synbiohub.org/public/igem/BBa_K678026/1
member: https://synbiohub.org/public/igem/BBa_K678063/1
member: https://synbiohub.org/public/igem/BBa_K678033/1
member: https://synbiohub.org/public/igem/BBa_I756015/1
member: https://synbiohub.org/public/igem/BBa_K863201/1
member: https://synbiohub.org/public/igem/BBa_K678024/1
member: https://synbiohub.org/public/igem/BBa_K133124/1
member: https://synbiohub.org/public/igem/BBa_K678070/1
member: https://synbiohub.org/public/igem/chassis_eukaryote_ppatens/1
member: https://synbiohub.org/public/igem/BBa_K284001/1
member: https://synbiohub.org/public/igem/BBa_K678045/1
member: https://synbiohub.org/public/igem/BBa_K678049/1
member: https://synbiohub.org/public/igem/BBa_K678010/1
member: https://synbiohub.org/public/igem/BBa_K678028/1
member: https://synbiohub.org/public/igem/BBa_K076017/1
member: https://synbiohub.org/public/igem/BBa_K105001/1
member: https://synbiohub.org/public/igem/BBa_K678015/1
member: https://synbiohub.org/public/igem/BBa_K678066/1
member: https://synbiohub.org/public/igem/BBa_K678036/1
member: https://synbiohub.org/public/igem/BBa_K678040/1
member: https://synbiohub.org/public/igem/BBa_J05215/1
member: https://synbiohub.org/public/igem/BBa_K678052/1
member: https://synbiohub.org/public/igem/chassis_eukaryote_yeast/1
member: https://synbiohub.org/public/igem/BBa_K157041/1
member: https://synbiohub.org/public/igem/BBa_K678065/1
member: https://synbiohub.org/public/igem/BBa_K157004/1
member: https://synbiohub.org/public/igem/BBa_K1484101/1
member: https://synbiohub.org/public/igem/BBa_K2057006/1
member: https://synbiohub.org/public/igem/BBa_K1852000/1
member: https://synbiohub.org/public/igem/BBa_K678035/1
member: https://synbiohub.org/public/igem/BBa_K678016/1
member: https://synbiohub.org/public/igem/BBa_I756021/1
member: https://synbiohub.org/public/igem/BBa_K678029/1
member: https://synbiohub.org/public/igem/BBa_K678059/1
member: https://synbiohub.org/public/igem/BBa_K157040/1
member: https://synbiohub.org/public/igem/BBa_I756017/1
member: https://synbiohub.org/public/igem/BBa_K678064/1
member: https://synbiohub.org/public/igem/BBa_K1484102/1
member: https://synbiohub.org/public/igem/BBa_K678017/1
member: https://synbiohub.org/public/igem/BBa_K678034/1
member: https://synbiohub.org/public/igem/BBa_K678050/1
member: https://synbiohub.org/public/igem/BBa_K678039/1
member: https://synbiohub.org/public/igem/BBa_I756016/1
member: https://synbiohub.org/public/igem/BBa_K1159300/1
member: https://synbiohub.org/public/igem/BBa_K863200/1
member: https://synbiohub.org/public/igem/chassis_eukaryote_nbenthamiana/1
member: https://synbiohub.org/public/igem/BBa_K678025/1
member: https://synbiohub.org/public/igem/BBa_I756020/1
member: https://synbiohub.org/public/igem/BBa_K678020/1
member: https://synbiohub.org/public/igem/BBa_K678048/1
member: https://synbiohub.org/public/igem/BBa_K678067/1
member: https://synbiohub.org/public/igem/chassis_eukaryote_human/1
member: https://synbiohub.org/public/igem/BBa_K678011/1
member: https://synbiohub.org/public/igem/BBa_K678027/1
member: https://synbiohub.org/public/igem/BBa_K678041/1
member: https://synbiohub.org/public/igem/BBa_K133120/1
member: https://synbiohub.org/public/igem/BBa_I756018/1
member: https://synbiohub.org/public/igem/BBa_K133125/1
member: https://synbiohub.org/public/igem/BBa_K678006/1
member: https://synbiohub.org/public/igem/BBa_K678062/1
member: https://synbiohub.org/public/igem/chassis_eukaryote_athaliana/1
member: https://synbiohub.org/public/igem/BBa_K678002/1
member: https://synbiohub.org/public/igem/BBa_K678032/1
member: https://synbiohub.org/public/igem/BBa_K809502/1
member: https://synbiohub.org/public/igem/BBa_I756019/1
member: https://synbiohub.org/public/igem/BBa_J05216/1
member: https://synbiohub.org/public/igem/BBa_K404093/1
member: https://synbiohub.org/public/igem/BBa_K678037/1

//chassis/organelle
type: Collection
persistent identity: chassis organelle
display id: chassis_organelle
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/chassis_organelle_mitochondrion/1
member: https://synbiohub.org/public/igem/chassis_organelle_chloroplast/1

//chassis
type: Collection
persistent identity: chassis
display id: chassis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: //chassis/organelle
member: Switch
member: //chassis/miscellaneous
member: //chassis/aferrooxidans
member: //chassis/eukaryote
member: //chassis/multihost
member: //chassis/bacteriophage
member: BBa_K1404007
member: BBa_K1404006
member: BBa_K1404008
member: //chassis/prokaryote

//chassis/multihost
type: Collection
persistent identity: chassis multihost
display id: chassis_multihost
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K823027/1
member: https://synbiohub.org/public/igem/BBa_J179000/1
member: https://synbiohub.org/public/igem/BBa_K1468001/1
member: https://synbiohub.org/public/igem/BBa_K823022/1
member: https://synbiohub.org/public/igem/BBa_K259009/1
member: https://synbiohub.org/public/igem/BBa_J179001/1
member: https://synbiohub.org/public/igem/BBa_K823026/1
member: https://synbiohub.org/public/igem/BBa_K1065203/1
member: https://synbiohub.org/public/igem/BBa_K823021/1
member: https://synbiohub.org/public/igem/BBa_K1023016/1
member: https://synbiohub.org/public/igem/BBa_K1468000/1
member: https://synbiohub.org/public/igem/BBa_K1023003/1
member: https://synbiohub.org/public/igem/BBa_J179002/1
member: https://synbiohub.org/public/igem/BBa_K1468004/1
member: https://synbiohub.org/public/igem/BBa_I742123/1
member: https://synbiohub.org/public/igem/BBa_K823025/1
member: https://synbiohub.org/public/igem/BBa_K1065204/1
member: https://synbiohub.org/public/igem/BBa_K259002/1
member: https://synbiohub.org/public/igem/BBa_K801031/1
member: https://synbiohub.org/public/igem/BBa_K1185004/1
member: https://synbiohub.org/public/igem/BBa_K823024/1
member: https://synbiohub.org/public/igem/BBa_K818000/1
member: https://synbiohub.org/public/igem/BBa_K801035/1
member: https://synbiohub.org/public/igem/BBa_K125340/1
member: https://synbiohub.org/public/igem/BBa_K1468002/1
member: https://synbiohub.org/public/igem/BBa_K125000/1
member: https://synbiohub.org/public/igem/BBa_K259008/1
member: https://synbiohub.org/public/igem/BBa_K801034/1
member: https://synbiohub.org/public/igem/BBa_K259004/1
member: https://synbiohub.org/public/igem/BBa_K823023/1

//chassis/prokaryote
type: Collection
persistent identity: chassis prokaryote
display id: chassis_prokaryote
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1468001/1
member: https://synbiohub.org/public/igem/BBa_K2057008/1
member: https://synbiohub.org/public/igem/BBa_K2057004/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_cyanobacterium/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_sespanaensis/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_subtilis/1
member: https://synbiohub.org/public/igem/BBa_K1468000/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_pmirabilis/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_pputida/1
member: https://synbiohub.org/public/igem/BBa_K1468004/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_vharveyi/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_pananatis/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_rradiobacter/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_bsubtilis/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_salmonella/1
member: https://synbiohub.org/public/igem/BBa_K862001/1
member: https://synbiohub.org/public/igem/BBa_K1979001/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_reuphora/1
member: https://synbiohub.org/public/igem/BBa_K1031911/1
member: https://synbiohub.org/public/igem/BBa_K862000/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_synechocystis/1
member: https://synbiohub.org/public/igem/BBa_K862002/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_mflorum/1
member: https://synbiohub.org/public/igem/BBa_K1468002/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_ecoli/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_bcepacia/1
member: https://synbiohub.org/public/igem/BBa_S05324/1
member: https://synbiohub.org/public/igem/chassis_prokaryote_lactobacillus/1
member: https://synbiohub.org/public/igem/BBa_K1031211/1
member: https://synbiohub.org/public/igem/BBa_K862003/1

//chassis/miscellaneous
type: Collection
persistent identity: chassis miscellaneous
display id: chassis_miscellaneous
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I723011/1
member: https://synbiohub.org/public/igem/BBa_I735010/1
member: https://synbiohub.org/public/igem/BBa_I765000/1
member: https://synbiohub.org/public/igem/BBa_K624000/1
member: https://synbiohub.org/public/igem/BBa_J64931/1
member: https://synbiohub.org/public/igem/BBa_K260018/1
member: https://synbiohub.org/public/igem/BBa_I723013/1
member: https://synbiohub.org/public/igem/BBa_J24001/1
member: https://synbiohub.org/public/igem/BBa_J48103/1
member: https://synbiohub.org/public/igem/BBa_I735008/1
member: https://synbiohub.org/public/igem/BBa_K624004/1
member: https://synbiohub.org/public/igem/BBa_J49000/1
member: https://synbiohub.org/public/igem/BBa_J16400/1
member: https://synbiohub.org/public/igem/BBa_J24000/1
member: https://synbiohub.org/public/igem/BBa_J48106/1
member: https://synbiohub.org/public/igem/BBa_I723018/1
member: https://synbiohub.org/public/igem/BBa_I735009/1
member: https://synbiohub.org/public/igem/BBa_I748001/1
member: https://synbiohub.org/public/igem/BBa_J54110/1
member: https://synbiohub.org/public/igem/BBa_K112126/1
member: https://synbiohub.org/public/igem/BBa_K112907/1
member: https://synbiohub.org/public/igem/BBa_K624003/1
member: https://synbiohub.org/public/igem/BBa_K624005/1
member: https://synbiohub.org/public/igem/BBa_J61045/1

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet
sequence annotation: BBa_K342000
sequence annotation: RBS Paris 2009
sequence annotation: His tag
sequence annotation: Beta sheet2
sequence annotation: GCT into GCC
sequence annotation: SEC signal
sequence annotation: CsgA-His2
sequence annotation: linker
sequence annotation: Linker
sequence annotation: CsgD signal
sequence annotation: Beta sheet5
sequence annotation: Beta sheet4
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Sec signal
sequence annotation: RBS Paris 2009
sequence annotation: CsgA-WT
sequence annotation: CsgG signal
sequence annotation: mutation
sequence annotation: Beta sheet4
sequence annotation: Beta sheet3
sequence annotation: Beta sheet5
sequence annotation: Beta sheet1
sequence annotation: Beta sheet2
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

Switch
type: ComponentDefinition
persistent identity: BBa K1185000
display id: BBa_K1185000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B.subtilis Rod to L-form Switch
creator: Vincent Leonardo, Christopher Wall and Robert Mckiernan
created: 2013-08-14T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick facilitates the switching of Bacillus subtilis cells between rod-shape to L-form, a cell wall-less form of bacteria. It consists of chloramphenicol acetyl-transferase (cat) gene and a xylose-controlled promoter (PxylR) which are flanked by ~300bp pbpB gene at the 5???end and ~300bp of murE gene at 3???end. These flanking sequences allows the integration of the BioBrick into the chromosome by homologous recombination replacing the region containing the end of pbpb gene and the beginning of the murE gene, which are involved in peptidoglycan biosynthesis. This event replaces the constituitive murE promoter with a PxylR upstream of the murE gene. Therefore, when xylose is present cell wall biosynthesis is switched on, giving rod cells. When xylose is not present, the cells will become L-forms. Simply transform the BioBrick into competent B. subtilis cells and through recombination it will integrate into cells chromosome. Plate out the transformants onto 1.5% lysogeny broth agar containing 5??g/ml Chloramphenicol and 0.8 %( w/v) xylose. This will give you rod cells containing biobrick. To get L-forms, inoculate some colonies into a solution containing an equal amount of nutrient broth and MSM. Also inoculate some transformants into the same media with the addition of 0.8 %( w/v) xylose to clarify that the switch BioBrick is working. Use microscopy to check the morphology of the cells in each media. L-forms will appear near spherical and can vary in size considerably.
mutable notes: There were 3 restriction sites that did not meet the RFC10 requirements, two EcoRI sites and one PstI. The first EcoRI restriction site was located 218bp into the whole BioBrick (inside pbpb region), we changed the sequence from GAATTC to GCATTC. The second restriction site was located 2287bp into the BioBrick in the spacer region between PxylR and murE, we changed it from GAATTC to GTATTC. The PstI site was located 197bp into the BioBrick in the pbpb region and was change from CTGCAG to CTTCAG.
mutable provenance: The part is the courtesy of Prof. Jeff Errington research group, and all the sequences come from the Bacillus subtilis genomic sequence. The pbpb section in the BioBrick was taken from end of the pbpb gene 1841-2151bp (309bp). Sequence acquired through microbes online VIMSS38051. The murE section was taken from the start of the murE gene 1-331bp. Sequence from microbes online VIMSS38053. The cat gene and PxylR gene were both come from the B. subtilis genome, but can also be found in the BioBrick Registry (BBa_K823020 and BBa_K143014).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_K823020
sequence annotation: murE RBS with spacers
sequence annotation: murE Homologous region
sequence annotation: pbpb Homologous region
sequence annotation: xylose-controlled promoter (PxylR)
sequence: https://synbiohub.org/public/igem/BBa_K1185000_sequence/1

BBa_K823020
type: SequenceAnnotation
persistent identity: annotation2331841
display id: annotation2331841
version: 1
top level: Switch
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331841/range2331841/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331841/range2331841/1
type: Range
persistent identity: range2331841
display id: range2331841
version: 1
direction: direction/forward
top level: Switch
start: 794
end: 1444
orientation: inline

murE RBS with spacers
type: SequenceAnnotation
persistent identity: annotation2331843
display id: annotation2331843
version: 1
top level: Switch
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331843/range2331843/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331843/range2331843/1
type: Range
persistent identity: range2331843
display id: range2331843
version: 1
direction: direction/forward
top level: Switch
start: 2228
end: 2292
orientation: inline

murE Homologous region
type: SequenceAnnotation
persistent identity: annotation2331844
display id: annotation2331844
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331844/range2331844/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331844/range2331844/1
type: Range
persistent identity: range2331844
display id: range2331844
version: 1
direction: direction/forward
top level: Switch
start: 2293
end: 2623
orientation: inline

pbpb Homologous region
type: SequenceAnnotation
persistent identity: annotation2331840
display id: annotation2331840
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331840/range2331840/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331840/range2331840/1
type: Range
persistent identity: range2331840
display id: range2331840
version: 1
direction: direction/forward
top level: Switch
start: 1
end: 309
orientation: inline

xylose-controlled promoter (PxylR)
type: SequenceAnnotation
persistent identity: annotation2331842
display id: annotation2331842
version: 1
top level: Switch
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1185000/annotation2331842/range2331842/1

https://synbiohub.org/public/igem/BBa_K1185000/annotation2331842/range2331842/1
type: Range
persistent identity: range2331842
display id: range2331842
version: 1
direction: direction/forward
top level: Switch
start: 2147
end: 2227
orientation: inline

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Sec signal
sequence annotation: BBa_K342000
sequence annotation: CsgG signal
sequence annotation: His tag
sequence annotation: Beta sheet5
sequence annotation: CsgA-His1
sequence annotation: Beta sheet 1
sequence annotation: Beta sheet3
sequence annotation: RBS Paris 2009
sequence annotation: Linker
sequence annotation: Beta sheet2
sequence annotation: GCT into GCC
sequence annotation: Beta sheet4
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline