//cds/selectionmarker
type: Collection
persistent identity: cds selectionmarker
display id: cds_selectionmarker
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K864150
member: pyrG casse
member: BBa_K1486032
member: ccdB
member: ZEC
member: 406
member: BBa_K394000
member: argB
member: PyrF
member: BBa_K1486031
member: BBa_K678041
member: CcdB
member: BBa_K1020012
member: //cds/selectionmarker/antibioticresistance

//cds/selectionmarker/antibioticresistance
type: Collection
persistent identity: cds selectionmarker antibioticresistance
display id: cds_selectionmarker_antibioticresistance
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: hygR
member: BBa_K1930003
member: BBa_K1015002
member: BBa_K1486027
member: CmR
member: BBa_K1207001
member: ptrA
member: BBa_K1015009
member: KanR Backw
member: BBa_K1323013
member: BBa_J23012
member: ampR
member: tetA(C)f
member: BBa_K319041
member: BBa_K1323012
member: BBa_K1021003
member: BBa_K1486029
member: BBa_K1486036
member: Aad9
member: kanR
member: BBa_K678022
member: BBa_K389011
member: BBa_K1486035
member: CiproR
member: BBa_K1323011
member: BBa_K156011
member: BBa_K678047
member: BBa_K1071001
member: BBa_T9010
member: BBa_K914018
member: ble
member: tetA(C)b
member: BBa_K1930007
member: BBa_K1015010
member: BBa_K1486033
member: BBa_K1486024
member: BBa_K1930002
member: Ori+Kan
member: BBa_K823020
member: NeoR
member: BBa_J133003
member: bleR
member: BBa_T9000
member: BBa_K914009
member: KanR
member: BBa_K394000
member: BBa_J133653
member: BBa_K1486030
member: CmR Backwa
member: BBa_K1486026

argB
type: ComponentDefinition
persistent identity: BBa K678043
display id: BBa_K678043
version: 1
was generated by: iGEM to SBOL conversion
description: argB cassette, selection marker for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Aspergillus nidulans gene argB encodes ornithine transcarbamylase and can be used as an auxotrophic marker in the filamentous fungi A. nidulans and A. niger. The successfull use of argB as a selection marker has been documented and described extensively in the literature.
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: Aspergillus nidulans genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: argB cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678043_sequence/1

argB cassette
type: SequenceAnnotation
persistent identity: annotation2127418
display id: annotation2127418
version: 1
top level: argB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678043/annotation2127418/range2127418/1

https://synbiohub.org/public/igem/BBa_K678043/annotation2127418/range2127418/1
type: Range
persistent identity: range2127418
display id: range2127418
version: 1
direction: direction/forward
top level: argB
start: 9
end: 2165
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127417
display id: annotation2127417
version: 1
top level: argB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678043/annotation2127417/range2127417/1

https://synbiohub.org/public/igem/BBa_K678043/annotation2127417/range2127417/1
type: Range
persistent identity: range2127417
display id: range2127417
version: 1
direction: direction/forward
top level: argB
start: 1
end: 8
orientation: inline

BBa_K1015010
type: ComponentDefinition
persistent identity: BBa K1015010
display id: BBa_K1015010
version: 1
was generated by: iGEM to SBOL conversion
description: Ampicillin resistance gene
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:51:04Z
mutable provenance: plasmid(pI258) of Staphylococcus aureus
owned by: james
owned by: myers
mutable description: Ampicillin resistance gene Ampicillin acts as an irreversible inhibitor of the enzyme transpeptidase, which is needed by bacteria to make their cell walls. It inhibits the third and final stage of bacterial cell wall synthesis in binary fission, which ultimately leads to cell lysis. This part includes a gene which has resistance to ampicillin. In our project, we use this part as a marker to select colonies of E.coli which succeeded in transformation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 13704
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: selection maker
star: false
creator: Marina Ikeno
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1015010_sequence/1

BBa_T9010
type: ComponentDefinition
persistent identity: BBa T9010
display id: BBa_T9010
version: 1
was generated by: iGEM to SBOL conversion
description: aacC2 with RBS
created: 2016-08-21T11:00:00Z
modified: 2016-08-22T11:02:40Z
mutable provenance: Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723
owned by: james
owned by: myers
mutable description: Translational unit including aacC2 gene encoding gentamicin resistance (BBa_T9000) with strong RBS BBa_B0034
discontinued: false
dominant: false
group u list: _1_
m user id: 33565
owner id: 33565
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Changed TAG stop codon to TAA
star: false
creator: Abigail Sison
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_T9000
sequence annotation: BBa_T9000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_T9010_sequence/1

BBa_B0034
type: Component
persistent identity: component2481504
display id: component2481504
version: 1
top level: BBa_T9010
access: public
definition: BBa_B0034

BBa_T9000
type: Component
persistent identity: component2481508
display id: component2481508
version: 1
top level: BBa_T9010
access: public
definition: BBa_T9000

BBa_T9000
type: SequenceAnnotation
persistent identity: annotation2481508
display id: annotation2481508
version: 1
top level: BBa_T9010
location: https://synbiohub.org/public/igem/BBa_T9010/annotation2481508/range2481508/1
component: BBa_T9000

https://synbiohub.org/public/igem/BBa_T9010/annotation2481508/range2481508/1
type: Range
persistent identity: range2481508
display id: range2481508
version: 1
top level: BBa_T9010
direction: direction/reverse
start: 19
end: 879
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481504
display id: annotation2481504
version: 1
top level: BBa_T9010
location: https://synbiohub.org/public/igem/BBa_T9010/annotation2481504/range2481504/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_T9010/annotation2481504/range2481504/1
type: Range
persistent identity: range2481504
display id: range2481504
version: 1
top level: BBa_T9010
direction: direction/reverse
start: 1
end: 12
orientation: inline

ble
type: ComponentDefinition
persistent identity: BBa K1033990
display id: BBa_K1033990
version: 1
was generated by: iGEM to SBOL conversion
description: Zeocin resistance cassette
created: 2013-09-02T11:00:00Z
modified: 2015-05-08T01:08:50Z
mutable provenance: Streptoalloteichus hindustanus, PDB: 1BYL
owned by: james
owned by: myers
mutable description: Zeocoin and bleomycin resistance cassette including promoter and coding sequence. It lacks a terminator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimized for E coli by GenScript.
star: false
creator: Erik Gullberg
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: ble
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1033990_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2334638
display id: annotation2334638
version: 1
top level: ble
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334638/range2334638/1
type: Range
persistent identity: range2334638
display id: range2334638
version: 1
top level: ble
direction: direction/forward
start: 58
end: 64
orientation: inline

ble
type: SequenceAnnotation
persistent identity: annotation2334639
display id: annotation2334639
version: 1
top level: ble
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334639/range2334639/1
type: Range
persistent identity: range2334639
display id: range2334639
version: 1
top level: ble
direction: direction/forward
start: 72
end: 446
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2334637
display id: annotation2334637
version: 1
top level: ble
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1

https://synbiohub.org/public/igem/BBa_K1033990/annotation2334637/range2334637/1
type: Range
persistent identity: range2334637
display id: range2334637
version: 1
top level: ble
direction: direction/forward
start: 2
end: 45
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: iGEM to SBOL conversion
description: virB promoter
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: contains already a RBS
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: rbs
sequence annotation: vir-box
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
top level: Pvir
direction: direction/forward
start: 89
end: 93
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
top level: Pvir
direction: direction/forward
start: 153
end: 157
orientation: inline

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
top level: Pvir
direction: direction/forward
start: 33
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
top level: Pvir
direction: direction/forward
start: 113
end: 117
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: james
owned by: myers
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is cloned into pSB1A2.
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
top level: CmR
direction: direction/forward
start: 1
end: 660
orientation: inline

BBa_K1930007
type: ComponentDefinition
persistent identity: BBa K1930007
display id: BBa_K1930007
version: 1
was generated by: iGEM to SBOL conversion
description: Ciprofloxacin resistance cassette in integration plasmid (BBa_K823023)
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:57Z
mutable provenance: Ciprofloxacin resistance cassette: Ordered as gBlock. IDT (Integrated DNA technologies). BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023.
owned by: james
owned by: myers
mutable description: This part is a ciprofloxacin resistance cassette. It is containing a PAtpI constitutive promoter, ciprofloxacin resistance gene qnrS1 and double terminator. The promoter PAtpI ([http://parts.igem.org/Part:BBa_K1930005 ''BBa_K1930005'']) is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. Ciprofloxacin resistance gene qnrS1 is found naturally in E. coli, and other Gram-negative strains. qnr genes code for pentapeptide repeat proteins.??These proteins reduce susceptibility to quinolones by protecting the complex of DNA and DNA gyrase enzyme from the inhibitory effect of quinolones. Double terminator ([http://parts.igem.org/Part:BBa_B0015 ''BBa_B0015'']) is the most commonly used terminator. For more information see here: http://parts.igem.org/Part:BBa_B0015.
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: design
star: false
creator: Barbora Waclawikova
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: BBa_K823023
sequence annotation: 2TER
sequence annotation: bla
sequence annotation: amyE
sequence annotation: cat
sequence annotation: BBa_B0015
sequence annotation: QnrS1
sequence annotation: P(AtpI)
sequence annotation: BBa_K1930005
sequence: https://synbiohub.org/public/igem/BBa_K1930007_sequence/1

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514184
display id: annotation2514184
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514184/range2514184/1
type: Range
persistent identity: range2514184
display id: range2514184
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1137
end: 7198
orientation: inline

2TER
type: SequenceAnnotation
persistent identity: annotation2514183
display id: annotation2514183
version: 1
top level: BBa_K1930007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514183/range2514183/1
type: Range
persistent identity: range2514183
display id: range2514183
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1008
end: 1136
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514187
display id: annotation2514187
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514187/range2514187/1
type: Range
persistent identity: range2514187
display id: range2514187
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 5476
end: 6336
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514188
display id: annotation2514188
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514188/range2514188/1
type: Range
persistent identity: range2514188
display id: range2514188
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 6661
end: 7181
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514185
display id: annotation2514185
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514185/range2514185/1
type: Range
persistent identity: range2514185
display id: range2514185
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1473
end: 2123
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2514198
display id: annotation2514198
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514198/range2514198/1
type: Range
persistent identity: range2514198
display id: range2514198
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1008
end: 1136
orientation: inline

QnrS1
type: SequenceAnnotation
persistent identity: annotation2514182
display id: annotation2514182
version: 1
top level: BBa_K1930007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514182/range2514182/1
type: Range
persistent identity: range2514182
display id: range2514182
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 351
end: 1007
orientation: inline

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2514180
display id: annotation2514180
version: 1
top level: BBa_K1930007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514180/range2514180/1
type: Range
persistent identity: range2514180
display id: range2514180
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1
end: 350
orientation: inline

BBa_K1930005
type: SequenceAnnotation
persistent identity: annotation2514181
display id: annotation2514181
version: 1
top level: BBa_K1930007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514181/range2514181/1
type: Range
persistent identity: range2514181
display id: range2514181
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 1
end: 350
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514186
display id: annotation2514186
version: 1
top level: BBa_K1930007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1

https://synbiohub.org/public/igem/BBa_K1930007/annotation2514186/range2514186/1
type: Range
persistent identity: range2514186
display id: range2514186
version: 1
top level: BBa_K1930007
direction: direction/forward
start: 2494
end: 3522
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa K389005
display id: BBa_K389005
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamycin resistance
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: BioBrick BBa_P1003
owned by: james
owned by: myers
mutable description: This is a kanamycin resistance part without promotor. It is made from the BioBrick BBa_P1003 and is used for screenings.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: we removed the promotor from the kanamycin resistance gene in BioBrick BBa_P1003
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_K389005_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078365
display id: annotation2078365
version: 1
top level: kanR
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1

https://synbiohub.org/public/igem/BBa_K389005/annotation2078365/range2078365/1
type: Range
persistent identity: range2078365
display id: range2078365
version: 1
top level: kanR
direction: direction/forward
start: 817
end: 819
orientation: inline

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2077058
display id: annotation2077058
version: 1
top level: kanR
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1

https://synbiohub.org/public/igem/BBa_K389005/annotation2077058/range2077058/1
type: Range
persistent identity: range2077058
display id: range2077058
version: 1
top level: kanR
direction: direction/forward
start: 1
end: 816
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: iGEM to SBOL conversion
description: Aad9 Spectinomycin Resistance Gene
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== <biblio> #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 765
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
top level: Aad9
direction: direction/forward
start: 769
end: 771
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
top level: Aad9
direction: direction/forward
start: 766
end: 768
orientation: inline

BBa_K1486030
type: ComponentDefinition
persistent identity: BBa K1486030
display id: BBa_K1486030
version: 1
was generated by: iGEM to SBOL conversion
description: rLucN + ADH1 terminator + Kanamycin resistance
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486037
component: BBa_K1486024
component: BBa_K1486019
component: BBa_K1486025
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486030_sequence/1

BBa_K1486037
type: Component
persistent identity: component2417716
display id: component2417716
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486037

BBa_K1486024
type: Component
persistent identity: component2417722
display id: component2417722
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486024

BBa_K1486019
type: Component
persistent identity: component2417717
display id: component2417717
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486019

BBa_K1486025
type: Component
persistent identity: component2417718
display id: component2417718
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486025

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417717
display id: annotation2417717
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
type: Range
persistent identity: range2417717
display id: range2417717
version: 1
top level: BBa_K1486030
direction: direction/reverse
start: 40
end: 369
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417716
display id: annotation2417716
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
type: Range
persistent identity: range2417716
display id: range2417716
version: 1
top level: BBa_K1486030
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417718
display id: annotation2417718
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
type: Range
persistent identity: range2417718
display id: range2417718
version: 1
top level: BBa_K1486030
direction: direction/reverse
start: 370
end: 557
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417722
display id: annotation2417722
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
type: Range
persistent identity: range2417722
display id: range2417722
version: 1
top level: BBa_K1486030
direction: direction/reverse
start: 558
end: 1914
orientation: inline

BBa_K1486033
type: ComponentDefinition
persistent identity: BBa K1486033
display id: BBa_K1486033
version: 1
was generated by: iGEM to SBOL conversion
description: R.reniformis luciferase + ADH1 terminator + CaUra3
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486020
component: BBa_K1486031
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486019
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486033_sequence/1

BBa_K1486020
type: Component
persistent identity: component2418784
display id: component2418784
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486020

BBa_K1486031
type: Component
persistent identity: component2418790
display id: component2418790
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486031

BBa_K1486025
type: Component
persistent identity: component2418786
display id: component2418786
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2418782
display id: component2418782
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486037

BBa_K1486019
type: Component
persistent identity: component2418783
display id: component2418783
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486019

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418790
display id: annotation2418790
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
type: Range
persistent identity: range2418790
display id: range2418790
version: 1
top level: BBa_K1486033
direction: direction/reverse
start: 1164
end: 2523
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418782
display id: annotation2418782
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
type: Range
persistent identity: range2418782
display id: range2418782
version: 1
top level: BBa_K1486033
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2418783
display id: annotation2418783
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
type: Range
persistent identity: range2418783
display id: range2418783
version: 1
top level: BBa_K1486033
direction: direction/reverse
start: 40
end: 369
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418786
display id: annotation2418786
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
type: Range
persistent identity: range2418786
display id: range2418786
version: 1
top level: BBa_K1486033
direction: direction/reverse
start: 976
end: 1163
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418784
display id: annotation2418784
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
type: Range
persistent identity: range2418784
display id: range2418784
version: 1
top level: BBa_K1486033
direction: direction/reverse
start: 370
end: 975
orientation: inline

BBa_K678041
type: ComponentDefinition
persistent identity: BBa K678041
display id: BBa_K678041
version: 1
was generated by: iGEM to SBOL conversion
description: pyrG-DR cassette, for selection of Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pyrG-DR cassette with directed repeats, for selection of Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: pDel2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: pyrG-DR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678041_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127413
display id: annotation2127413
version: 1
top level: BBa_K678041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678041/annotation2127413/range2127413/1

https://synbiohub.org/public/igem/BBa_K678041/annotation2127413/range2127413/1
type: Range
persistent identity: range2127413
display id: range2127413
version: 1
direction: direction/forward
top level: BBa_K678041
start: 1
end: 8
orientation: inline

pyrG-DR cassette
type: SequenceAnnotation
persistent identity: annotation2127414
display id: annotation2127414
version: 1
top level: BBa_K678041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678041/annotation2127414/range2127414/1

https://synbiohub.org/public/igem/BBa_K678041/annotation2127414/range2127414/1
type: Range
persistent identity: range2127414
display id: range2127414
version: 1
direction: direction/forward
top level: BBa_K678041
start: 9
end: 2168
orientation: inline

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rev primer J31007
sequence annotation: Fwd primer J31007
sequence annotation: tetA(C) forward
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

hygR
type: ComponentDefinition
persistent identity: BBa K678021
display id: BBa_K678021
version: 1
was generated by: iGEM to SBOL conversion
description: Hygromycin cassette
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid pcDNA3.1/Hygro from Invitrogen
owned by: james
owned by: myers
mutable description: Hygromycin cassette for selection of mammalian cells. The selection cassette contains the SV40 promoter, the hygromycin resistance gene, and the SV40 poly A as terminator
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plu'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Hygromycin cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678021_sequence/1

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2127357
display id: annotation2127357
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127357/range2127357/1
type: Range
persistent identity: range2127357
display id: range2127357
version: 1
top level: hygR
direction: direction/forward
start: 9
end: 1759
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127356
display id: annotation2127356
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1

https://synbiohub.org/public/igem/BBa_K678021/annotation2127356/range2127356/1
type: Range
persistent identity: range2127356
display id: range2127356
version: 1
top level: hygR
direction: direction/forward
start: 1
end: 8
orientation: inline

406
type: ComponentDefinition
persistent identity: BBa K398406
display id: BBa_K398406
version: 1
was generated by: iGEM to SBOL conversion
description: Solvent tolerance cluster
creator: Hugo Federico Cueto Rojas and Ramon van der Valk
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6321
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12
mutable notes: Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.
mutable provenance: Genomic sequence obtained from NCBI, the sequences were enhanced for iGEM purposes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: stop
sequence annotation: BBa_J23100
sequence annotation: BBa_J61107
sequence annotation: PhPFD-alpha
sequence annotation: BBa_K398400
sequence annotation: PhPFD-beta
sequence annotation: BBa_K398401
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_K398406_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2085534
display id: annotation2085534
version: 1
top level: 406
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085534/range2085534/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085534/range2085534/1
type: Range
persistent identity: range2085534
display id: range2085534
version: 1
direction: direction/forward
top level: 406
start: 544
end: 546
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2085530
display id: annotation2085530
version: 1
top level: 406
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085530/range2085530/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085530/range2085530/1
type: Range
persistent identity: range2085530
display id: range2085530
version: 1
direction: direction/forward
top level: 406
start: 515
end: 517
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2085535
display id: annotation2085535
version: 1
top level: 406
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085535/range2085535/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085535/range2085535/1
type: Range
persistent identity: range2085535
display id: range2085535
version: 1
direction: direction/forward
top level: 406
start: 895
end: 897
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2085526
display id: annotation2085526
version: 1
top level: 406
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085526/range2085526/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085526/range2085526/1
type: Range
persistent identity: range2085526
display id: range2085526
version: 1
direction: direction/reverse
top level: 406
start: 1
end: 35
orientation: inline

BBa_J61107
type: SequenceAnnotation
persistent identity: annotation2085532
display id: annotation2085532
version: 1
top level: 406
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085532/range2085532/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085532/range2085532/1
type: Range
persistent identity: range2085532
display id: range2085532
version: 1
direction: direction/reverse
top level: 406
start: 526
end: 537
orientation: inline

BBa_J61107
type: SequenceAnnotation
persistent identity: annotation2085527
display id: annotation2085527
version: 1
top level: 406
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085527/range2085527/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085527/range2085527/1
type: Range
persistent identity: range2085527
display id: range2085527
version: 1
direction: direction/reverse
top level: 406
start: 44
end: 55
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2085528
display id: annotation2085528
version: 1
top level: 406
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085528/range2085528/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085528/range2085528/1
type: Range
persistent identity: range2085528
display id: range2085528
version: 1
direction: direction/forward
top level: 406
start: 62
end: 64
orientation: inline

PhPFD-alpha
type: SequenceAnnotation
persistent identity: annotation2085529
display id: annotation2085529
version: 1
top level: 406
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085529/range2085529/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085529/range2085529/1
type: Range
persistent identity: range2085529
display id: range2085529
version: 1
direction: direction/forward
top level: 406
start: 62
end: 517
orientation: inline

BBa_K398400
type: SequenceAnnotation
persistent identity: annotation2085531
display id: annotation2085531
version: 1
top level: 406
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085531/range2085531/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085531/range2085531/1
type: Range
persistent identity: range2085531
display id: range2085531
version: 1
direction: direction/reverse
top level: 406
start: 62
end: 517
orientation: inline

PhPFD-beta
type: SequenceAnnotation
persistent identity: annotation2085533
display id: annotation2085533
version: 1
top level: 406
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085533/range2085533/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085533/range2085533/1
type: Range
persistent identity: range2085533
display id: range2085533
version: 1
direction: direction/forward
top level: 406
start: 544
end: 897
orientation: inline

BBa_K398401
type: SequenceAnnotation
persistent identity: annotation2085536
display id: annotation2085536
version: 1
top level: 406
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085536/range2085536/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085536/range2085536/1
type: Range
persistent identity: range2085536
display id: range2085536
version: 1
direction: direction/reverse
top level: 406
start: 544
end: 897
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation2085537
display id: annotation2085537
version: 1
top level: 406
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K398406/annotation2085537/range2085537/1

https://synbiohub.org/public/igem/BBa_K398406/annotation2085537/range2085537/1
type: Range
persistent identity: range2085537
display id: range2085537
version: 1
direction: direction/forward
top level: 406
start: 36
end: 41
orientation: inline

BBa_K389011
type: ComponentDefinition
persistent identity: BBa K389011
display id: BBa_K389011
version: 1
was generated by: iGEM to SBOL conversion
description: VirA screening device
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
mutable description: This part contains: - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' - a kanamycin resistance under the control of a ''vir'' promotor This device is used to screen ''virA'' receptor mutants. The ''virA'' receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more ''virG'' is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23110
component: BBa_K389003
component: BBa_K389002
component: BBa_K389005
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_K389005
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389011_sequence/1

BBa_J23110
type: Component
persistent identity: component2078366
display id: component2078366
version: 1
top level: BBa_K389011
access: public
definition: BBa_J23110

BBa_K389003
type: Component
persistent identity: component2078379
display id: component2078379
version: 1
top level: BBa_K389011
access: public
definition: Pvir

BBa_K389002
type: Component
persistent identity: component2078370
display id: component2078370
version: 1
top level: BBa_K389011
access: public
definition: virG

BBa_K389005
type: Component
persistent identity: component2078382
display id: component2078382
version: 1
top level: BBa_K389011
access: public
definition: kanR

BBa_B0010
type: Component
persistent identity: component2078371
display id: component2078371
version: 1
top level: BBa_K389011
access: public
definition: BBa_B0010

BBa_B0010
type: Component
persistent identity: component2078373
display id: component2078373
version: 1
top level: BBa_K389011
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078371
display id: annotation2078371
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078371/range2078371/1
type: Range
persistent identity: range2078371
display id: range2078371
version: 1
top level: BBa_K389011
direction: direction/forward
start: 792
end: 871
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078373
display id: annotation2078373
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389011/annotation2078373/range2078373/1
type: Range
persistent identity: range2078373
display id: range2078373
version: 1
top level: BBa_K389011
direction: direction/forward
start: 880
end: 959
orientation: inline

BBa_K389005
type: SequenceAnnotation
persistent identity: annotation2078382
display id: annotation2078382
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
component: BBa_K389005

https://synbiohub.org/public/igem/BBa_K389011/annotation2078382/range2078382/1
type: Range
persistent identity: range2078382
display id: range2078382
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 1132
end: 1950
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2078370
display id: annotation2078370
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389011/annotation2078370/range2078370/1
type: Range
persistent identity: range2078370
display id: range2078370
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 44
end: 783
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2078366
display id: annotation2078366
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389011/annotation2078366/range2078366/1
type: Range
persistent identity: range2078366
display id: range2078366
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2078379
display id: annotation2078379
version: 1
top level: BBa_K389011
location: https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389011/annotation2078379/range2078379/1
type: Range
persistent identity: range2078379
display id: range2078379
version: 1
top level: BBa_K389011
direction: direction/reverse
start: 968
end: 1125
orientation: inline

BBa_K1486036
type: ComponentDefinition
persistent identity: BBa K1486036
display id: BBa_K1486036
version: 1
was generated by: iGEM to SBOL conversion
description: rLucC + ADH1 terminator + CaUra3 cassette
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486025
component: BBa_K1486037
component: BBa_K1486031
component: BBa_K1486020
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486031
sequence: https://synbiohub.org/public/igem/BBa_K1486036_sequence/1

BBa_K1486025
type: Component
persistent identity: component2418339
display id: component2418339
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486025

BBa_K1486037
type: Component
persistent identity: component2418337
display id: component2418337
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486037

BBa_K1486031
type: Component
persistent identity: component2418343
display id: component2418343
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486031

BBa_K1486020
type: Component
persistent identity: component2418338
display id: component2418338
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486020

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418337
display id: annotation2418337
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
type: Range
persistent identity: range2418337
display id: range2418337
version: 1
top level: BBa_K1486036
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418339
display id: annotation2418339
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
type: Range
persistent identity: range2418339
display id: range2418339
version: 1
top level: BBa_K1486036
direction: direction/reverse
start: 646
end: 833
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418338
display id: annotation2418338
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
type: Range
persistent identity: range2418338
display id: range2418338
version: 1
top level: BBa_K1486036
direction: direction/reverse
start: 40
end: 645
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418343
display id: annotation2418343
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
type: Range
persistent identity: range2418343
display id: range2418343
version: 1
top level: BBa_K1486036
direction: direction/reverse
start: 834
end: 2193
orientation: inline

BBa_K1486031
type: ComponentDefinition
persistent identity: BBa K1486031
display id: BBa_K1486031
version: 1
was generated by: iGEM to SBOL conversion
description: CaUra3 selection marker
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF terminator
sequence annotation: Ura3
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486031_sequence/1

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2417787
display id: annotation2417787
version: 1
top level: BBa_K1486031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1
type: Range
persistent identity: range2417787
display id: range2417787
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1162
end: 1360
orientation: inline

Ura3
type: SequenceAnnotation
persistent identity: annotation2417786
display id: annotation2417786
version: 1
top level: BBa_K1486031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1
type: Range
persistent identity: range2417786
display id: range2417786
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 345
end: 1157
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2417785
display id: annotation2417785
version: 1
top level: BBa_K1486031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1
type: Range
persistent identity: range2417785
display id: range2417785
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1
end: 344
orientation: inline

BBa_K1323011
type: ComponentDefinition
persistent identity: BBa K1323011
display id: BBa_K1323011
version: 1
was generated by: iGEM to SBOL conversion
description: Erythromycin resistance coding sequence
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:42:45Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Julia Manalil
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ermM CDS (Erythromycin resistance)
sequence: https://synbiohub.org/public/igem/BBa_K1323011_sequence/1

ermM CDS (Erythromycin resistance)
type: SequenceAnnotation
persistent identity: annotation2428789
display id: annotation2428789
version: 1
top level: BBa_K1323011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323011/annotation2428789/range2428789/1

https://synbiohub.org/public/igem/BBa_K1323011/annotation2428789/range2428789/1
type: Range
persistent identity: range2428789
display id: range2428789
version: 1
top level: BBa_K1323011
direction: direction/forward
start: 1
end: 735
orientation: inline

BBa_K319041
type: ComponentDefinition
persistent identity: BBa K319041
display id: BBa_K319041
version: 1
was generated by: iGEM to SBOL conversion
description: NatMX cassette
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: n
owned by: james
owned by: myers
mutable description: This is the cassette (promoter+coding region+terminator) that imparts resistance to nourseothricin in yeast.
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: false
creator: Afnan Azizi
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TEF1 promoter
sequence annotation: TEF1 terminator
sequence annotation: NatMX ORF
sequence: https://synbiohub.org/public/igem/BBa_K319041_sequence/1

TEF1 promoter
type: SequenceAnnotation
persistent identity: annotation2109281
display id: annotation2109281
version: 1
top level: BBa_K319041
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109281/range2109281/1
type: Range
persistent identity: range2109281
display id: range2109281
version: 1
top level: BBa_K319041
direction: direction/forward
start: 1
end: 379
orientation: inline

TEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2109283
display id: annotation2109283
version: 1
top level: BBa_K319041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109283/range2109283/1
type: Range
persistent identity: range2109283
display id: range2109283
version: 1
top level: BBa_K319041
direction: direction/forward
start: 956
end: 1190
orientation: inline

NatMX ORF
type: SequenceAnnotation
persistent identity: annotation2109282
display id: annotation2109282
version: 1
top level: BBa_K319041
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1

https://synbiohub.org/public/igem/BBa_K319041/annotation2109282/range2109282/1
type: Range
persistent identity: range2109282
display id: range2109282
version: 1
top level: BBa_K319041
direction: direction/forward
start: 380
end: 955
orientation: inline

BBa_K1930003
type: ComponentDefinition
persistent identity: BBa K1930003
display id: BBa_K1930003
version: 1
was generated by: iGEM to SBOL conversion
description: Encrypted message sequence in integration plasmid (BBa_K823023)
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:01:43Z
mutable provenance: Message sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
star: false
creator: Barbora Waclawikova
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: BBa_K1930001
sequence annotation: BBa_K823023
sequence annotation: cat
sequence annotation: message sequence
sequence annotation: bla
sequence annotation: amyE
sequence: https://synbiohub.org/public/igem/BBa_K1930003_sequence/1

BBa_K1930001
type: SequenceAnnotation
persistent identity: annotation2514197
display id: annotation2514197
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2514197/range2514197/1
type: Range
persistent identity: range2514197
display id: range2514197
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1
end: 556
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2513444
display id: annotation2513444
version: 1
top level: BBa_K1930003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513444/range2513444/1
type: Range
persistent identity: range2513444
display id: range2513444
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 557
end: 6618
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2513446
display id: annotation2513446
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513446/range2513446/1
type: Range
persistent identity: range2513446
display id: range2513446
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 893
end: 1543
orientation: inline

message sequence
type: SequenceAnnotation
persistent identity: annotation2513445
display id: annotation2513445
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513445/range2513445/1
type: Range
persistent identity: range2513445
display id: range2513445
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1
end: 556
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2513448
display id: annotation2513448
version: 1
top level: BBa_K1930003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513448/range2513448/1
type: Range
persistent identity: range2513448
display id: range2513448
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 4896
end: 5756
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513447
display id: annotation2513447
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513447/range2513447/1
type: Range
persistent identity: range2513447
display id: range2513447
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 1914
end: 2942
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2513449
display id: annotation2513449
version: 1
top level: BBa_K1930003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1

https://synbiohub.org/public/igem/BBa_K1930003/annotation2513449/range2513449/1
type: Range
persistent identity: range2513449
display id: range2513449
version: 1
top level: BBa_K1930003
direction: direction/forward
start: 6081
end: 6601
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_K1071001
type: ComponentDefinition
persistent identity: BBa K1071001
display id: BBa_K1071001
version: 1
was generated by: iGEM to SBOL conversion
description: ble resistance (eucaryotic)
created: 2013-09-18T11:00:00Z
modified: 2016-01-28T01:51:30Z
mutable provenance: Phaeodactylum tricornutum
owned by: james
owned by: myers
mutable description: Gives resistance against the antibiotic zeocin for eucaryotes.
discontinued: false
dominant: false
experience: experience/None
group u list: _1380_
m user id: 4206
owner id: 18233
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No mutations added
star: false
creator: Team Marburg 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1071001_sequence/1

BBa_K1486035
type: ComponentDefinition
persistent identity: BBa K1486035
display id: BBa_K1486035
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486034
component: BBa_K1486037
component: BBa_K1486025
component: BBa_K1486031
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486034
sequence annotation: BBa_K1486025
sequence: https://synbiohub.org/public/igem/BBa_K1486035_sequence/1

BBa_K1486034
type: Component
persistent identity: component2418330
display id: component2418330
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486034

BBa_K1486037
type: Component
persistent identity: component2418328
display id: component2418328
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486037

BBa_K1486025
type: Component
persistent identity: component2418331
display id: component2418331
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486025

BBa_K1486031
type: Component
persistent identity: component2418335
display id: component2418335
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486031

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418335
display id: annotation2418335
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
type: Range
persistent identity: range2418335
display id: range2418335
version: 1
top level: BBa_K1486035
direction: direction/reverse
start: 303
end: 1662
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418328
display id: annotation2418328
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
type: Range
persistent identity: range2418328
display id: range2418328
version: 1
top level: BBa_K1486035
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486034
type: SequenceAnnotation
persistent identity: annotation2418330
display id: annotation2418330
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
component: BBa_K1486034

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
type: Range
persistent identity: range2418330
display id: range2418330
version: 1
top level: BBa_K1486035
direction: direction/reverse
start: 40
end: 114
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418331
display id: annotation2418331
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
type: Range
persistent identity: range2418331
display id: range2418331
version: 1
top level: BBa_K1486035
direction: direction/reverse
start: 115
end: 302
orientation: inline

BBa_K125530
type: ComponentDefinition
persistent identity: BBa K125530
display id: BBa_K125530
version: 1
was generated by: iGEM to SBOL conversion
description: aadA gene with lacIQ promoter and RBS
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part was amplified from pRL1383a, an RSF1010 derived plasmid.
owned by: james
owned by: myers
mutable description: The ''aadA'' gene confers resistance to Spectinomycin and Streptomycin. The lacIQ promoter and an [[Part:BBa_B0034|RBS] were added. This part is functional without a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part will be tested on varying concentrations of antibiotics.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K125520
component: BBa_B0034
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_K125520
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K125530_sequence/1

BBa_K125520
type: Component
persistent identity: component1988107
display id: component1988107
version: 1
top level: BBa_K125530
access: public
definition: aada

BBa_B0034
type: Component
persistent identity: component1988106
display id: component1988106
version: 1
top level: BBa_K125530
access: public
definition: BBa_B0034

BBa_I14032
type: Component
persistent identity: component1988104
display id: component1988104
version: 1
top level: BBa_K125530
access: public
definition: P(Lac) IQ

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1988104
display id: annotation1988104
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988104/range1988104/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125530/annotation1988104/range1988104/1
type: Range
persistent identity: range1988104
display id: range1988104
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_K125520
type: SequenceAnnotation
persistent identity: annotation1988107
display id: annotation1988107
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988107/range1988107/1
component: BBa_K125520

https://synbiohub.org/public/igem/BBa_K125530/annotation1988107/range1988107/1
type: Range
persistent identity: range1988107
display id: range1988107
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 64
end: 855
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1988106
display id: annotation1988106
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988106/range1988106/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125530/annotation1988106/range1988106/1
type: Range
persistent identity: range1988106
display id: range1988106
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 46
end: 57
orientation: inline

KanR
type: ComponentDefinition
persistent identity: BBa J31003
display id: BBa_J31003
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance forward (KanF) [cf. BBa_J23012 & BBa_J31002]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AK3
owned by: james
owned by: myers
mutable description: If there is a promoter and an RBS before this region, the cell will express Kanamycin resistance. As it has full biobricks, you can easily add KanR to an existing part without changing the plasmid vector,
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is cloned in plasmid pSB1A2.
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KanR Gene
sequence: https://synbiohub.org/public/igem/BBa_J31003_sequence/1

KanR Gene
type: SequenceAnnotation
persistent identity: annotation1884996
display id: annotation1884996
version: 1
top level: KanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1

https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1
type: Range
persistent identity: range1884996
display id: range1884996
version: 1
top level: KanR
direction: direction/forward
start: 1
end: 816
orientation: inline

BBa_K864150
type: ComponentDefinition
persistent identity: BBa K864150
display id: BBa_K864150
version: 1
was generated by: iGEM to SBOL conversion
description: Cat-SacB cassette for scarless chromosomal knockout
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cat-SacB Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921
mutable notes: -
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K322921
component: BBa_P1004
sequence annotation: BBa_P1004
sequence annotation: BBa_K322921
sequence: https://synbiohub.org/public/igem/BBa_K864150_sequence/1

BBa_K322921
type: Component
persistent identity: component2197683
display id: component2197683
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_K322921/1

BBa_P1004
type: Component
persistent identity: component2197679
display id: component2197679
version: 1
top level: BBa_K864150
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2197679
display id: annotation2197679
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K864150/annotation2197679/range2197679/1
type: Range
persistent identity: range2197679
display id: range2197679
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 1
end: 769
orientation: inline

BBa_K322921
type: SequenceAnnotation
persistent identity: annotation2197683
display id: annotation2197683
version: 1
top level: BBa_K864150
location: https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
component: BBa_K322921

https://synbiohub.org/public/igem/BBa_K864150/annotation2197683/range2197683/1
type: Range
persistent identity: range2197683
display id: range2197683
version: 1
direction: direction/reverse
top level: BBa_K864150
start: 778
end: 2214
orientation: inline

BBa_K156011
type: ComponentDefinition
persistent identity: BBa K156011
display id: BBa_K156011
version: 1
was generated by: iGEM to SBOL conversion
description: aadA (streptomycin 3'-adenyltransferase)
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
mutable provenance: aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438).
owned by: james
owned by: myers
mutable description: This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker.
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
star: true
creator: George McArthur IV
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156011_sequence/1

BBa_K1486019
type: ComponentDefinition
persistent identity: BBa K1486019
display id: BBa_K1486019
version: 1
was generated by: iGEM to SBOL conversion
description: rLuc[1]
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: to edit
owned by: james
owned by: myers
mutable description: his is the first part of the split Renilla luciferase. To be used with fLuc[2].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486019_sequence/1

BBa_K1486034
type: ComponentDefinition
persistent identity: BBa K1486034
display id: BBa_K1486034
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast optimized superfolder GFP C-terminus (215-238)
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Yeast optimized sfGFP C-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486034_sequence/1

Yeast optimized sfGFP C-terminus
type: SequenceAnnotation
persistent identity: annotation2417784
display id: annotation2417784
version: 1
top level: BBa_K1486034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1

https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1
type: Range
persistent identity: range2417784
display id: range2417784
version: 1
top level: BBa_K1486034
direction: direction/forward
start: 1
end: 75
orientation: inline

BBa_T9000
type: ComponentDefinition
persistent identity: BBa T9000
display id: BBa_T9000
version: 1
was generated by: iGEM to SBOL conversion
description: aacC2
created: 2016-08-21T11:00:00Z
modified: 2016-08-22T10:11:14Z
mutable provenance: Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723
owned by: james
owned by: myers
mutable description: E.coli R-plasmid aacC2 gene for aminoglycoside-(3)-N-acetyltransferase activity AKA Gentamicin resistance
discontinued: false
dominant: false
group u list: _1_
m user id: 33565
owner id: 33565
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Changed TAG stop codon to TAA
star: false
creator: Abigail Sison
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: aacC2 coding region
sequence annotation: TAA Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_T9000_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2481500
display id: annotation2481500
version: 1
top level: BBa_T9000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481500/range2481500/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481500/range2481500/1
type: Range
persistent identity: range2481500
display id: range2481500
version: 1
top level: BBa_T9000
direction: direction/forward
start: 1
end: 3
orientation: inline

aacC2 coding region
type: SequenceAnnotation
persistent identity: annotation2481501
display id: annotation2481501
version: 1
top level: BBa_T9000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481501/range2481501/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481501/range2481501/1
type: Range
persistent identity: range2481501
display id: range2481501
version: 1
top level: BBa_T9000
direction: direction/forward
start: 1
end: 861
orientation: inline

TAA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2481502
display id: annotation2481502
version: 1
top level: BBa_T9000
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_T9000/annotation2481502/range2481502/1

https://synbiohub.org/public/igem/BBa_T9000/annotation2481502/range2481502/1
type: Range
persistent identity: range2481502
display id: range2481502
version: 1
top level: BBa_T9000
direction: direction/forward
start: 859
end: 861
orientation: inline

BBa_K914018
type: ComponentDefinition
persistent identity: BBa K914018
display id: BBa_K914018
version: 1
was generated by: iGEM to SBOL conversion
description: P1003** Kan resistant gene with 2 Amber Codon
created: 2012-10-24T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: This part is made from the BBa_K914009 part from which I add a second mutation with the QuickChange Site-directed mutagenesis kit, Agilent.
owned by: james
owned by: myers
mutable description: The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: I have need to design the primers that will introduce a mutation from a serine that was closer to the TAG codon (either 1, 2 or 3 mutations, here it was 1 and 2 mutations for both mutations), and it is necessary to have the amber codon in the middle of the coding sequence, not in the beginning, to be not interpreted as a alternative start codon for instance.
star: false
creator: Jean Cury
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: kanamycin resistance
sequence annotation: Ser133->Amber codon
sequence annotation: Ser203->Amber mutation
sequence annotation: BBa_K914009
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_K914018_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212595
display id: annotation2212595
version: 1
top level: BBa_K914018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1
type: Range
persistent identity: range2212595
display id: range2212595
version: 1
top level: BBa_K914018
direction: direction/forward
start: 149
end: 967
orientation: inline

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212596
display id: annotation2212596
version: 1
top level: BBa_K914018
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1
type: Range
persistent identity: range2212596
display id: range2212596
version: 1
top level: BBa_K914018
direction: direction/forward
start: 546
end: 546
orientation: inline

Ser203->Amber mutation
type: SequenceAnnotation
persistent identity: annotation2212599
display id: annotation2212599
version: 1
top level: BBa_K914018
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1
type: Range
persistent identity: range2212599
display id: range2212599
version: 1
top level: BBa_K914018
direction: direction/forward
start: 609
end: 610
orientation: inline

BBa_K914009
type: SequenceAnnotation
persistent identity: annotation2212597
display id: annotation2212597
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1
type: Range
persistent identity: range2212597
display id: range2212597
version: 1
top level: BBa_K914018
direction: direction/reverse
start: 1
end: 967
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212594
display id: annotation2212594
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1
type: Range
persistent identity: range2212594
display id: range2212594
version: 1
top level: BBa_K914018
direction: direction/forward
start: 1
end: 967
orientation: inline

bleR
type: ComponentDefinition
persistent identity: BBa K678044
display id: BBa_K678044
version: 1
was generated by: iGEM to SBOL conversion
description: bleR cassette, selection marker for Aspergillus nidulans
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: pAN8-1
owned by: myers
owned by: james
mutable description: bleR cassette, antibiotic selection marker for Aspergillus nidulans
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: bleR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678044_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127419
display id: annotation2127419
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127419/range2127419/1
type: Range
persistent identity: range2127419
display id: range2127419
version: 1
top level: bleR
direction: direction/forward
start: 1
end: 8
orientation: inline

bleR cassette
type: SequenceAnnotation
persistent identity: annotation2127420
display id: annotation2127420
version: 1
top level: bleR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1

https://synbiohub.org/public/igem/BBa_K678044/annotation2127420/range2127420/1
type: Range
persistent identity: range2127420
display id: range2127420
version: 1
top level: bleR
direction: direction/forward
start: 9
end: 1663
orientation: inline

pyrG casse
type: ComponentDefinition
persistent identity: BBa K678040
display id: BBa_K678040
version: 1
was generated by: iGEM to SBOL conversion
description: pyrG cassette, for selection of Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pyrG cassette, for selection of Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: plasmid pDel2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: pyrG-cassette
sequence: https://synbiohub.org/public/igem/BBa_K678040_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127411
display id: annotation2127411
version: 1
top level: pyrG casse
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678040/annotation2127411/range2127411/1

https://synbiohub.org/public/igem/BBa_K678040/annotation2127411/range2127411/1
type: Range
persistent identity: range2127411
display id: range2127411
version: 1
direction: direction/forward
top level: pyrG casse
start: 1
end: 8
orientation: inline

pyrG-cassette
type: SequenceAnnotation
persistent identity: annotation2127412
display id: annotation2127412
version: 1
top level: pyrG casse
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678040/annotation2127412/range2127412/1

https://synbiohub.org/public/igem/BBa_K678040/annotation2127412/range2127412/1
type: Range
persistent identity: range2127412
display id: range2127412
version: 1
direction: direction/forward
top level: pyrG casse
start: 9
end: 1456
orientation: inline

BBa_K801012
type: ComponentDefinition
persistent identity: BBa K801012
display id: BBa_K801012
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 yeast terminator
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: ADH1 terminator of ''S. cerevisiae''
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Georg Sch??tzinger
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: ADH1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801012_sequence/1

ADH1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202145
display id: annotation2202145
version: 1
top level: BBa_K801012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1

https://synbiohub.org/public/igem/BBa_K801012/annotation2202145/range2202145/1
type: Range
persistent identity: range2202145
display id: range2202145
version: 1
top level: BBa_K801012
direction: direction/forward
start: 1
end: 349
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: iGEM to SBOL conversion
description: Mutated virG
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: Stop codon
sequence annotation: virG
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
top level: virG
direction: direction/forward
start: 4
end: 8
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
top level: virG
direction: direction/forward
start: 738
end: 740
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
top level: virG
direction: direction/forward
start: 12
end: 737
orientation: inline

Ori+Kan
type: ComponentDefinition
persistent identity: BBa K320004
display id: BBa_K320004
version: 1
was generated by: iGEM to SBOL conversion
description: Origin for Asaia and resistance to Kanamycin
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: t
owned by: james
owned by: myers
mutable description: t
discontinued: false
dominant: false
experience: experience/Issues
group u list: _439_
m user id: 0
owner id: 7149
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: t
star: false
creator: Perrudet Christian
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Asaia replication origin
sequence annotation: BBa_K320000
sequence annotation: KanR
sequence: https://synbiohub.org/public/igem/BBa_K320004_sequence/1

Asaia replication origin
type: SequenceAnnotation
persistent identity: annotation2091397
display id: annotation2091397
version: 1
top level: Ori+Kan
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091397/range2091397/1
type: Range
persistent identity: range2091397
display id: range2091397
version: 1
top level: Ori+Kan
direction: direction/forward
start: 355
end: 1029
orientation: inline

BBa_K320000
type: SequenceAnnotation
persistent identity: annotation2088717
display id: annotation2088717
version: 1
top level: Ori+Kan
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2088717/range2088717/1
type: Range
persistent identity: range2088717
display id: range2088717
version: 1
top level: Ori+Kan
direction: direction/reverse
start: 1
end: 1399
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2091398
display id: annotation2091398
version: 1
top level: Ori+Kan
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1

https://synbiohub.org/public/igem/BBa_K320004/annotation2091398/range2091398/1
type: Range
persistent identity: range2091398
display id: range2091398
version: 1
top level: Ori+Kan
direction: direction/forward
start: 1400
end: 2372
orientation: inline

BBa_K1486029
type: ComponentDefinition
persistent identity: BBa K1486029
display id: BBa_K1486029
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486024
component: BBa_K1486028
component: BBa_K1486037
component: BBa_K1486025
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486028
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486029_sequence/1

BBa_K1486024
type: Component
persistent identity: component2417704
display id: component2417704
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486024

BBa_K1486028
type: Component
persistent identity: component2417699
display id: component2417699
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486028

BBa_K1486037
type: Component
persistent identity: component2417697
display id: component2417697
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486037

BBa_K1486025
type: Component
persistent identity: component2417700
display id: component2417700
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486025

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417700
display id: annotation2417700
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
type: Range
persistent identity: range2417700
display id: range2417700
version: 1
top level: BBa_K1486029
direction: direction/reverse
start: 682
end: 869
orientation: inline

BBa_K1486028
type: SequenceAnnotation
persistent identity: annotation2417699
display id: annotation2417699
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
component: BBa_K1486028

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
type: Range
persistent identity: range2417699
display id: range2417699
version: 1
top level: BBa_K1486029
direction: direction/reverse
start: 40
end: 681
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417697
display id: annotation2417697
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
type: Range
persistent identity: range2417697
display id: range2417697
version: 1
top level: BBa_K1486029
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417704
display id: annotation2417704
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
type: Range
persistent identity: range2417704
display id: range2417704
version: 1
top level: BBa_K1486029
direction: direction/reverse
start: 870
end: 2226
orientation: inline

BBa_K1015002
type: ComponentDefinition
persistent identity: BBa K1015002
display id: BBa_K1015002
version: 1
was generated by: iGEM to SBOL conversion
description: streptmycin resisitance gene (can PCR ampicillin resistance gene primer)
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T01:50:11Z
mutable provenance: aminoglycoside resistance gene (aadA) come from R100.1 plasmid.
owned by: james
owned by: myers
mutable description: streptmycin resisitance gene(aadA) This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ.
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: antibiotic resistance
star: false
creator: Kento Tominaga
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aadA(streptomycin resistance gene)
sequence annotation: blaZ fragment(promoter contain)
sequence annotation: blaZ fragment
sequence: https://synbiohub.org/public/igem/BBa_K1015002_sequence/1

aadA(streptomycin resistance gene)
type: SequenceAnnotation
persistent identity: annotation2342880
display id: annotation2342880
version: 1
top level: BBa_K1015002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1
type: Range
persistent identity: range2342880
display id: range2342880
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 119
end: 911
orientation: inline

blaZ fragment(promoter contain)
type: SequenceAnnotation
persistent identity: annotation2342881
display id: annotation2342881
version: 1
top level: BBa_K1015002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1
type: Range
persistent identity: range2342881
display id: range2342881
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 1
end: 118
orientation: inline

blaZ fragment
type: SequenceAnnotation
persistent identity: annotation2342888
display id: annotation2342888
version: 1
top level: BBa_K1015002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1
type: Range
persistent identity: range2342888
display id: range2342888
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 911
end: 1030
orientation: inline

BBa_K1930002
type: ComponentDefinition
persistent identity: BBa K1930002
display id: BBa_K1930002
version: 1
was generated by: iGEM to SBOL conversion
description: Encryption key sequence in integration plasmid (BBa_K823023)
created: 2016-10-15T11:00:00Z
modified: 2016-10-17T09:00:28Z
mutable provenance: Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding BBa_K823023: Created by iGEM Munich 2012. http://parts.igem.org/Part:BBa_K823023
owned by: james
owned by: myers
mutable description: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
discontinued: true
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation.
star: false
creator: Eike Mahlandt
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: amyE
sequence annotation: key sequence
sequence annotation: cat
sequence annotation: BBa_K823023
sequence annotation: BBa_K1930000
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K1930002_sequence/1

amyE
type: SequenceAnnotation
persistent identity: annotation2514192
display id: annotation2514192
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514192/range2514192/1
type: Range
persistent identity: range2514192
display id: range2514192
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 1358
end: 2386
orientation: inline

key sequence
type: SequenceAnnotation
persistent identity: annotation2514195
display id: annotation2514195
version: 1
top level: BBa_K1930002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514195/range2514195/1
type: Range
persistent identity: range2514195
display id: range2514195
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 6063
end: 6206
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2514191
display id: annotation2514191
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514191/range2514191/1
type: Range
persistent identity: range2514191
display id: range2514191
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 337
end: 987
orientation: inline

BBa_K823023
type: SequenceAnnotation
persistent identity: annotation2514190
display id: annotation2514190
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514190/range2514190/1
type: Range
persistent identity: range2514190
display id: range2514190
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 1
end: 6062
orientation: inline

BBa_K1930000
type: SequenceAnnotation
persistent identity: annotation2514196
display id: annotation2514196
version: 1
top level: BBa_K1930002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514196/range2514196/1
type: Range
persistent identity: range2514196
display id: range2514196
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 6063
end: 6206
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2514193
display id: annotation2514193
version: 1
top level: BBa_K1930002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514193/range2514193/1
type: Range
persistent identity: range2514193
display id: range2514193
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 4340
end: 5200
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2514194
display id: annotation2514194
version: 1
top level: BBa_K1930002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1

https://synbiohub.org/public/igem/BBa_K1930002/annotation2514194/range2514194/1
type: Range
persistent identity: range2514194
display id: range2514194
version: 1
top level: BBa_K1930002
direction: direction/forward
start: 5525
end: 6045
orientation: inline

aada
type: ComponentDefinition
persistent identity: BBa K125520
display id: BBa_K125520
version: 1
was generated by: iGEM to SBOL conversion
description: aadA gene
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part was amplified from pRL1383a, an RSF1010 derived plasmid.
owned by: james
owned by: myers
mutable description: The ''aadA'' gene confers resistance to Spectinomycin and Streptomycin. The lacIQ promoter and an [[Part:BBa_B0034|RBS] were added. This part is functional without a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will be tested on varying concentrations of antibiotics.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K125520_sequence/1

BBa_K1020012
type: ComponentDefinition
persistent identity: BBa K1020012
display id: BBa_K1020012
version: 1
was generated by: iGEM to SBOL conversion
description: Alkane Selector for intracellular alkane production
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1020010
component: BBa_K1020003
sequence annotation: BBa_K1020010
sequence annotation: BBa_K1020003
sequence: https://synbiohub.org/public/igem/BBa_K1020012_sequence/1

BBa_K1020010
type: Component
persistent identity: component2373789
display id: component2373789
version: 1
top level: BBa_K1020012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020010/1

BBa_K1020003
type: Component
persistent identity: component2373802
display id: component2373802
version: 1
top level: BBa_K1020012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020003/1

BBa_K1020010
type: SequenceAnnotation
persistent identity: annotation2373789
display id: annotation2373789
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
component: BBa_K1020010

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
type: Range
persistent identity: range2373789
display id: range2373789
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1
end: 1529
orientation: inline

BBa_K1020003
type: SequenceAnnotation
persistent identity: annotation2373802
display id: annotation2373802
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
component: BBa_K1020003

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
type: Range
persistent identity: range2373802
display id: range2373802
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1538
end: 2662
orientation: inline

BBa_K1486024
type: ComponentDefinition
persistent identity: BBa K1486024
display id: BBa_K1486024
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast kanamycin resistance
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF promoter
sequence annotation: KanR
sequence annotation: TEF terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486024_sequence/1

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2416942
display id: annotation2416942
version: 1
top level: BBa_K1486024
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416942/range2416942/1
type: Range
persistent identity: range2416942
display id: range2416942
version: 1
top level: BBa_K1486024
direction: direction/forward
start: 1
end: 344
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2416943
display id: annotation2416943
version: 1
top level: BBa_K1486024
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416943/range2416943/1
type: Range
persistent identity: range2416943
display id: range2416943
version: 1
top level: BBa_K1486024
direction: direction/forward
start: 345
end: 1154
orientation: inline

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2416944
display id: annotation2416944
version: 1
top level: BBa_K1486024
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1

https://synbiohub.org/public/igem/BBa_K1486024/annotation2416944/range2416944/1
type: Range
persistent identity: range2416944
display id: range2416944
version: 1
top level: BBa_K1486024
direction: direction/forward
start: 1159
end: 1357
orientation: inline

BBa_K1486028
type: ComponentDefinition
persistent identity: BBa K1486028
display id: BBa_K1486028
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast optimized sfGFP N-terminus (1-214)
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: split N term of sfGFP To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: yeast optimized sfGFP N-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486028_sequence/1

yeast optimized sfGFP N-terminus
type: SequenceAnnotation
persistent identity: annotation2417081
display id: annotation2417081
version: 1
top level: BBa_K1486028
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1

https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1
type: Range
persistent identity: range2417081
display id: range2417081
version: 1
top level: BBa_K1486028
direction: direction/forward
start: 1
end: 642
orientation: inline

BBa_K1323013
type: ComponentDefinition
persistent identity: BBa K1323013
display id: BBa_K1323013
version: 1
was generated by: iGEM to SBOL conversion
description: aadD - Kanamycin Resistance Gene Coding Sequence
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Julia Manalil
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aadD
sequence: https://synbiohub.org/public/igem/BBa_K1323013_sequence/1

aadD
type: SequenceAnnotation
persistent identity: annotation2428783
display id: annotation2428783
version: 1
top level: BBa_K1323013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1

https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1
type: Range
persistent identity: range2428783
display id: range2428783
version: 1
top level: BBa_K1323013
direction: direction/forward
start: 1
end: 726
orientation: inline

ZEC
type: ComponentDefinition
persistent identity: BBa K1018001
display id: BBa_K1018001
version: 1
was generated by: iGEM to SBOL conversion
description: she ble gene conferring Zeocin resistance
creator: KhaiCheng Kiew
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1325_
m user id: 0
owner id: 17919
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Zeocin is an antibiotic for mammalian transfection
mutable notes: None
mutable provenance: Streptoalloteichus hindustanus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1018001_sequence/1

BBa_J23012
type: ComponentDefinition
persistent identity: BBa J23012
display id: BBa_J23012
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin R open-reading-frame basic part
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
owned by: james
owned by: myers
mutable description: it is a Spec resistant biobrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _52_
m user id: 0
owner id: 363
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: JL1 Forward EcoRI oligo to bioBrick SpecR: gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc JL2 Reverse SpeI oligo to bioBrick SpecR: ccgctactagtCAGCAACGATGTTACGCAGC
star: false
creator: Will Bosworth
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SpR Spectinomycin Resistance
sequence: https://synbiohub.org/public/igem/BBa_J23012_sequence/1

SpR Spectinomycin Resistance
type: SequenceAnnotation
persistent identity: annotation1966465
display id: annotation1966465
version: 1
top level: BBa_J23012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1

https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
type: Range
persistent identity: range1966465
display id: range1966465
version: 1
top level: BBa_J23012
direction: direction/reverse
start: 46
end: 837
orientation: inline

BBa_K823020
type: ComponentDefinition
persistent identity: BBa K823020
display id: BBa_K823020
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (cat)
created: 2012-08-14T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: Plasmid: Bacillus subtilis reporter vector pAC6
owned by: james
owned by: myers
mutable description: This part codes for the chloramphenicol acetyltransferase (cat) gene used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions.
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site.
star: false
creator: Simon Kelterborn
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823020_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179570
display id: annotation2179570
version: 1
top level: BBa_K823020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1

https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1
type: Range
persistent identity: range2179570
display id: range2179570
version: 1
top level: BBa_K823020
direction: direction/forward
start: 1
end: 645
orientation: inline

BBa_K1015009
type: ComponentDefinition
persistent identity: BBa K1015009
display id: BBa_K1015009
version: 1
was generated by: iGEM to SBOL conversion
description: Chloramphenicol resistance gene+FRT
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:53Z
mutable provenance: Streptomyces coelicolor A3(2)
owned by: james
owned by: myers
mutable description: Chloramphenicol resistance gene Chloramphenicol is effective against a wide variety of Gram-positive and Gram-negative bacteria, including most anaerobic organisms.This part includes a gene which has resistance to chloramphenicol. In our project, we use this part as a marker to select colonies of E.coli which succeeded in transformation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 13704
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: selection marker
star: false
creator: Marina Ikeno
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cm
sequence annotation: FRT
sequence: https://synbiohub.org/public/igem/BBa_K1015009_sequence/1

Cm
type: SequenceAnnotation
persistent identity: annotation2347534
display id: annotation2347534
version: 1
top level: BBa_K1015009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1015009/annotation2347534/range2347534/1

https://synbiohub.org/public/igem/BBa_K1015009/annotation2347534/range2347534/1
type: Range
persistent identity: range2347534
display id: range2347534
version: 1
top level: BBa_K1015009
direction: direction/forward
start: 395
end: 1055
orientation: inline

FRT
type: SequenceAnnotation
persistent identity: annotation2347569
display id: annotation2347569
version: 1
top level: BBa_K1015009
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K1015009/annotation2347569/range2347569/1

https://synbiohub.org/public/igem/BBa_K1015009/annotation2347569/range2347569/1
type: Range
persistent identity: range2347569
display id: range2347569
version: 1
top level: BBa_K1015009
direction: direction/forward
start: 47
end: 80
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
creator: Jonas Demeulemeester
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Deleted
bookmark: true
mutable description: Coding region for the ccdB (control of cell death) gene.
mutable notes: just one stop codon in the end
mutable provenance: P1010
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cds
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
direction: direction/forward
top level: ccdB
start: 304
end: 306
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
direction: direction/forward
top level: ccdB
start: 1
end: 303
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
direction: direction/forward
top level: ccdB
start: 1
end: 3
orientation: inline

BBa_K1021003
type: ComponentDefinition
persistent identity: BBa K1021003
display id: BBa_K1021003
version: 1
was generated by: iGEM to SBOL conversion
description: bar (bialaphos resistance)
created: 2013-08-29T11:00:00Z
modified: 2016-01-28T01:51:16Z
mutable provenance: pBARGPE1 from the FGSC (http://www.fgsc.net/fgn/pall.html)
owned by: james
owned by: myers
mutable description: The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.
discontinued: false
dominant: false
group u list: _1328_
m user id: 4206
owner id: 13516
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The plasmid sequence was unknown so extensive sequencing was carried out in order to extract it from its original vector, confirm BioBrick compatibility, and transfer it to pSB1C3.
star: false
creator: Swati Sureka
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1021003_sequence/1

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

KanR Backw
type: ComponentDefinition
persistent identity: BBa J31002
display id: BBa_J31002
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance backwards (KanB) [cf. BBa_J23012 & BBa_J31003]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AK3
owned by: myers
owned by: james
mutable description: Codes the reverse of the Kanamycin Resistance gene. When the region is flipped, Kanamycin resistance will be expressed as long as a promoter and RBS are in front of the region.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of KanR gene
sequence: https://synbiohub.org/public/igem/BBa_J31002_sequence/1

Reverse of KanR gene
type: SequenceAnnotation
persistent identity: annotation1884995
display id: annotation1884995
version: 1
top level: KanR Backw
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1

https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1
type: Range
persistent identity: range1884995
display id: range1884995
version: 1
top level: KanR Backw
direction: direction/reverse
start: 1
end: 816
orientation: inline

BBa_K1486026
type: ComponentDefinition
persistent identity: BBa K1486026
display id: BBa_K1486026
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP + Kanamycin resistance for yeast
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: BBa_K1486023
sequence annotation: Pbs2 flanking
sequence annotation: 8 a.a linker
sequence annotation: Adh1 terminator
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486026_sequence/1

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417049
display id: annotation2417049
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1
type: Range
persistent identity: range2417049
display id: range2417049
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 75
end: 791
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417052
display id: annotation2417052
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1
type: Range
persistent identity: range2417052
display id: range2417052
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 2485
end: 2535
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417047
display id: annotation2417047
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1
type: Range
persistent identity: range2417047
display id: range2417047
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 1
end: 50
orientation: inline

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417048
display id: annotation2417048
version: 1
top level: BBa_K1486026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1
type: Range
persistent identity: range2417048
display id: range2417048
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 51
end: 74
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2417050
display id: annotation2417050
version: 1
top level: BBa_K1486026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1
type: Range
persistent identity: range2417050
display id: range2417050
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 811
end: 999
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417051
display id: annotation2417051
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1
type: Range
persistent identity: range2417051
display id: range2417051
version: 1
top level: BBa_K1486026
direction: direction/forward
start: 1073
end: 2430
orientation: inline

CiproR
type: ComponentDefinition
persistent identity: BBa K1930004
display id: BBa_K1930004
version: 1
was generated by: iGEM to SBOL conversion
description: Ciprofloxacin resistance cassette
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T04:58:30Z
mutable provenance: Ordered as gBlock. IDT (Integrated DNA technologies).
owned by: james
owned by: myers
mutable description: QnrS1 resistance cassette BioBrick BBa_K1930004.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: To design a QnrS1 resistance cassette BioBrick BBa_K1930004 we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original QnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+QnrS1+2TER.
star: false
creator: Mareike Schmidt
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0015
sequence annotation: BBa_K1930005
sequence annotation: P(AtpI)
sequence annotation: Qnrs1
sequence annotation: 2TER
sequence: https://synbiohub.org/public/igem/BBa_K1930004_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2513506
display id: annotation2513506
version: 1
top level: CiproR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513506/range2513506/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513506/range2513506/1
type: Range
persistent identity: range2513506
display id: range2513506
version: 1
top level: CiproR
direction: direction/forward
start: 1008
end: 1136
orientation: inline

BBa_K1930005
type: SequenceAnnotation
persistent identity: annotation2513503
display id: annotation2513503
version: 1
top level: CiproR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513503/range2513503/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513503/range2513503/1
type: Range
persistent identity: range2513503
display id: range2513503
version: 1
top level: CiproR
direction: direction/forward
start: 1
end: 350
orientation: inline

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2513502
display id: annotation2513502
version: 1
top level: CiproR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513502/range2513502/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513502/range2513502/1
type: Range
persistent identity: range2513502
display id: range2513502
version: 1
top level: CiproR
direction: direction/forward
start: 1
end: 350
orientation: inline

Qnrs1
type: SequenceAnnotation
persistent identity: annotation2513504
display id: annotation2513504
version: 1
top level: CiproR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513504/range2513504/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513504/range2513504/1
type: Range
persistent identity: range2513504
display id: range2513504
version: 1
top level: CiproR
direction: direction/forward
start: 351
end: 1007
orientation: inline

2TER
type: SequenceAnnotation
persistent identity: annotation2513505
display id: annotation2513505
version: 1
top level: CiproR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1930004/annotation2513505/range2513505/1

https://synbiohub.org/public/igem/BBa_K1930004/annotation2513505/range2513505/1
type: Range
persistent identity: range2513505
display id: range2513505
version: 1
top level: CiproR
direction: direction/forward
start: 1008
end: 1136
orientation: inline

BBa_K678022
type: ComponentDefinition
persistent identity: BBa K678022
display id: BBa_K678022
version: 1
was generated by: iGEM to SBOL conversion
description: Neomycin cassette
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
mutable description: Resistance cassette for selection of mammalian cells. The cassette contains a SV40 promoter, the neomycin resistance gene, and the SV40 poly A as a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker 5
sequence annotation: Neomycin cassette
sequence: https://synbiohub.org/public/igem/BBa_K678022_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127358
display id: annotation2127358
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127358/range2127358/1
type: Range
persistent identity: range2127358
display id: range2127358
version: 1
top level: BBa_K678022
direction: direction/forward
start: 1
end: 8
orientation: inline

Neomycin cassette
type: SequenceAnnotation
persistent identity: annotation2127359
display id: annotation2127359
version: 1
top level: BBa_K678022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1

https://synbiohub.org/public/igem/BBa_K678022/annotation2127359/range2127359/1
type: Range
persistent identity: range2127359
display id: range2127359
version: 1
top level: BBa_K678022
direction: direction/forward
start: 9
end: 1512
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: P(Lac) IQ
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

hygR
type: ComponentDefinition
persistent identity: BBa K678042
display id: BBa_K678042
version: 1
was generated by: iGEM to SBOL conversion
description: hygromycin cassette for Aspergillus nidulans
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: pCB1001
owned by: james
owned by: myers
mutable description: hygromycin cassette for Aspergillus nidulans
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: hygR cassette
sequence: https://synbiohub.org/public/igem/BBa_K678042_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127415
display id: annotation2127415
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127415/range2127415/1
type: Range
persistent identity: range2127415
display id: range2127415
version: 1
top level: hygR
direction: direction/forward
start: 1
end: 8
orientation: inline

hygR cassette
type: SequenceAnnotation
persistent identity: annotation2127416
display id: annotation2127416
version: 1
top level: hygR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1

https://synbiohub.org/public/igem/BBa_K678042/annotation2127416/range2127416/1
type: Range
persistent identity: range2127416
display id: range2127416
version: 1
top level: hygR
direction: direction/forward
start: 9
end: 1363
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

CmR Backwa
type: ComponentDefinition
persistent identity: BBa J31004
display id: BBa_J31004
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: myers
owned by: james
mutable description: Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was cloned pSB1A2.
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31004_sequence/1

Reverse of CmR gene
type: SequenceAnnotation
persistent identity: annotation1884997
display id: annotation1884997
version: 1
top level: CmR Backwa
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1

https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1
type: Range
persistent identity: range1884997
display id: range1884997
version: 1
top level: CmR Backwa
direction: direction/forward
start: 1
end: 660
orientation: inline

BBa_K1486025
type: ComponentDefinition
persistent identity: BBa K1486025
display id: BBa_K1486025
version: 1
was generated by: iGEM to SBOL conversion
description: ADH1 Terminator
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Adh1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486025_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2418776
display id: annotation2418776
version: 1
top level: BBa_K1486025
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1

https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1
type: Range
persistent identity: range2418776
display id: range2418776
version: 1
top level: BBa_K1486025
direction: direction/forward
start: 1
end: 188
orientation: inline

BBa_K914009
type: ComponentDefinition
persistent identity: BBa K914009
display id: BBa_K914009
version: 1
was generated by: iGEM to SBOL conversion
description: P1003* Ser133->Amber Codon
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: this part comes from BBa_P1003
owned by: james
owned by: myers
mutable description: The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: I made the single point mutation with a quick change directed mutagenesis kit.
star: false
creator: Jean Cury
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: kanamycin resistance
sequence annotation: Ser133->Amber codon
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_K914009_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212601
display id: annotation2212601
version: 1
top level: BBa_K914009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1
type: Range
persistent identity: range2212601
display id: range2212601
version: 1
top level: BBa_K914009
direction: direction/forward
start: 149
end: 967
orientation: inline

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212602
display id: annotation2212602
version: 1
top level: BBa_K914009
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1
type: Range
persistent identity: range2212602
display id: range2212602
version: 1
top level: BBa_K914009
direction: direction/forward
start: 546
end: 546
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212600
display id: annotation2212600
version: 1
top level: BBa_K914009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1
type: Range
persistent identity: range2212600
display id: range2212600
version: 1
top level: BBa_K914009
direction: direction/forward
start: 1
end: 967
orientation: inline

CcdB
type: ComponentDefinition
persistent identity: BBa K1318000
display id: BBa_K1318000
version: 1
was generated by: iGEM to SBOL conversion
description: CcdB toxin
creator: Laurence Van Melderen
created: 2014-10-01T11:00:00Z
modified: 2015-07-09T11:28:18Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _1693_
m user id: 4206
owner id: 20061
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable description: CcdB is a toxin that poisons and kills E. coli by inhibiting the DNA gyrase and inducing double strand breaks. CcdB activity can be inhibited by the CcdA antitoxin. This gene is under the control of a galactose inducible promoter
mutable notes: CcdB is toxic so it has to be expressed in conjunction with CcdA or not on a galactose-containing medium.
mutable provenance: E. coli fertility factor (F plasmid)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CcdB
sequence annotation: CcdB start
sequence annotation: CcdB stop
sequence: https://synbiohub.org/public/igem/BBa_K1318000_sequence/1

CcdB
type: SequenceAnnotation
persistent identity: annotation2416919
display id: annotation2416919
version: 1
top level: CcdB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1
type: Range
persistent identity: range2416919
display id: range2416919
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 309
orientation: inline

CcdB start
type: SequenceAnnotation
persistent identity: annotation2416917
display id: annotation2416917
version: 1
top level: CcdB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1
type: Range
persistent identity: range2416917
display id: range2416917
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 3
orientation: inline

CcdB stop
type: SequenceAnnotation
persistent identity: annotation2416918
display id: annotation2416918
version: 1
top level: CcdB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1
type: Range
persistent identity: range2416918
display id: range2416918
version: 1
direction: direction/forward
top level: CcdB
start: 307
end: 309
orientation: inline

NeoR
type: ComponentDefinition
persistent identity: BBa K1313004
display id: BBa_K1313004
version: 1
was generated by: iGEM to SBOL conversion
description: Neomycin Resistance
created: 2014-10-11T11:00:00Z
modified: 2016-01-28T01:43:08Z
mutable provenance: This gene was obtained from pCDNA 3.1 myc His(A).
owned by: james
owned by: myers
mutable description: Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media. This gene can be also used as a selective gene for mammalian cells using gentamicin.
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 4206
owner id: 19941
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Pst1 site cuts gene.
star: false
creator: Carlos Meza
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1313004_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K1323012
type: ComponentDefinition
persistent identity: BBa K1323012
display id: BBa_K1323012
version: 1
was generated by: iGEM to SBOL conversion
description: Spc - Spectinomycin Resistance Gene Coding Sequence
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:19Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Julia Manalil
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spc
sequence: https://synbiohub.org/public/igem/BBa_K1323012_sequence/1

spc
type: SequenceAnnotation
persistent identity: annotation2428780
display id: annotation2428780
version: 1
top level: BBa_K1323012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1

https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1
type: Range
persistent identity: range2428780
display id: range2428780
version: 1
top level: BBa_K1323012
direction: direction/forward
start: 1
end: 783
orientation: inline

BBa_K1486032
type: ComponentDefinition
persistent identity: BBa K1486032
display id: BBa_K1486032
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP + ADH1 terminator + Ura3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 8 a.a linker
sequence annotation: Hog1 5' flanking region
sequence annotation: Hog1 3' flanking region
sequence annotation: BBa_K1486023
sequence annotation: BBa_K1486031
sequence annotation: ADH1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486032_sequence/1

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417800
display id: annotation2417800
version: 1
top level: BBa_K1486032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1
type: Range
persistent identity: range2417800
display id: range2417800
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 48
end: 71
orientation: inline

Hog1 5' flanking region
type: SequenceAnnotation
persistent identity: annotation2417799
display id: annotation2417799
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1
type: Range
persistent identity: range2417799
display id: range2417799
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1
end: 47
orientation: inline

Hog1 3' flanking region
type: SequenceAnnotation
persistent identity: annotation2417805
display id: annotation2417805
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1
type: Range
persistent identity: range2417805
display id: range2417805
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 2494
end: 2544
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417801
display id: annotation2417801
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1
type: Range
persistent identity: range2417801
display id: range2417801
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 72
end: 804
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2417804
display id: annotation2417804
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1
type: Range
persistent identity: range2417804
display id: range2417804
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1070
end: 2430
orientation: inline

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation2417803
display id: annotation2417803
version: 1
top level: BBa_K1486032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1
type: Range
persistent identity: range2417803
display id: range2417803
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 808
end: 996
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa K1073000
display id: BBa_K1073000
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance
created: 2013-09-15T11:00:00Z
modified: 2016-01-28T01:50:42Z
mutable provenance: The part was derived from BBa_P1002 ampicillin resistance cassette. The sequence for the beta-lactamase was gained by PCR of the original BBa_P1002 with appropiate primers.
owned by: james
owned by: myers
mutable description: This sequence codes for a beta-lactamase, which degrades ampicillin. There is no RBS or other information attached, only the sequence for the enzyme plus prefix and suffix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1382_
m user id: 4206
owner id: 15674
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The primer sequences can be found on iGEM Team Braunschweig's wiki.
star: false
creator: iGEM Team Braunschweig 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1073000_sequence/1

BBa_K678047
type: ComponentDefinition
persistent identity: BBa K678047
display id: BBa_K678047
version: 1
was generated by: iGEM to SBOL conversion
description: Ampicillin cassette
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: From plasmid pcDNA3.1 from Invitrogen
owned by: james
owned by: myers
mutable description: For selection of E. coli transformants, contains promoter, ampR gene, and terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ampR cassette
sequence annotation: Linker 5
sequence: https://synbiohub.org/public/igem/BBa_K678047_sequence/1

ampR cassette
type: SequenceAnnotation
persistent identity: annotation2127426
display id: annotation2127426
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127426/range2127426/1
type: Range
persistent identity: range2127426
display id: range2127426
version: 1
top level: BBa_K678047
direction: direction/forward
start: 9
end: 867
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127425
display id: annotation2127425
version: 1
top level: BBa_K678047
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1

https://synbiohub.org/public/igem/BBa_K678047/annotation2127425/range2127425/1
type: Range
persistent identity: range2127425
display id: range2127425
version: 1
top level: BBa_K678047
direction: direction/forward
start: 1
end: 8
orientation: inline

BBa_J133653
type: ComponentDefinition
persistent identity: BBa J133653
display id: BBa_J133653
version: 1
was generated by: iGEM to SBOL conversion
description: aadA Cassette + Flanked by TLTerminators (missing RBS)
created: 2011-07-21T11:00:00Z
modified: 2015-08-31T04:08:30Z
mutable provenance: http://partsregistry.org/wiki/index.php/Part:BBa_J133003
owned by: james
owned by: myers
mutable description: KanR gene BBa_P1003 with BsmBI removed, it has duplicated promoter region for kanR since it was amplified from pSB3K5m which is a pSB3K5 derivative. Flanked with bidirectional translational Terminators BBa_J133422 (prefix) and BBa_B0042 (postfix)
discontinued: false
dominant: false
experience: experience/None
group u list: _634_
m user id: 0
owner id: 2680
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Norman Wang
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: BBa_J133422
sequence annotation: BBa_B0042
sequence annotation: BBa I14032 P(Lac) IQ
sequence annotation: E81V mutation GAG to GTG
sequence annotation: BBa K125520 Aminoglycoside 3"-Adenylyltransferase (aadA1) Gene CDS
sequence: https://synbiohub.org/public/igem/BBa_J133653_sequence/1

BBa_J133422
type: SequenceAnnotation
persistent identity: annotation2125650
display id: annotation2125650
version: 1
top level: BBa_J133653
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125650/range2125650/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125650/range2125650/1
type: Range
persistent identity: range2125650
display id: range2125650
version: 1
top level: BBa_J133653
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2125653
display id: annotation2125653
version: 1
top level: BBa_J133653
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125653/range2125653/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125653/range2125653/1
type: Range
persistent identity: range2125653
display id: range2125653
version: 1
top level: BBa_J133653
direction: direction/forward
start: 848
end: 859
orientation: inline

BBa I14032 P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation2125651
display id: annotation2125651
version: 1
top level: BBa_J133653
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125651/range2125651/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125651/range2125651/1
type: Range
persistent identity: range2125651
display id: range2125651
version: 1
top level: BBa_J133653
direction: direction/forward
start: 13
end: 49
orientation: inline

E81V mutation GAG to GTG
type: SequenceAnnotation
persistent identity: annotation2125654
display id: annotation2125654
version: 1
top level: BBa_J133653
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125654/range2125654/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125654/range2125654/1
type: Range
persistent identity: range2125654
display id: range2125654
version: 1
top level: BBa_J133653
direction: direction/forward
start: 296
end: 298
orientation: inline

BBa K125520 Aminoglycoside 3"-Adenylyltransferase (aadA1) Gene CDS
type: SequenceAnnotation
persistent identity: annotation2125652
display id: annotation2125652
version: 1
top level: BBa_J133653
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J133653/annotation2125652/range2125652/1

https://synbiohub.org/public/igem/BBa_J133653/annotation2125652/range2125652/1
type: Range
persistent identity: range2125652
display id: range2125652
version: 1
top level: BBa_J133653
direction: direction/forward
start: 56
end: 847
orientation: inline

BBa_K1207001
type: ComponentDefinition
persistent identity: BBa K1207001
display id: BBa_K1207001
version: 1
was generated by: iGEM to SBOL conversion
description: Macrolide-Lincosamide-Streptogramin B Resistance Marker
created: 2013-09-11T11:00:00Z
modified: 2016-01-28T01:51:46Z
mutable provenance: This part was cloned from the plasmid pIKM1.
owned by: james
owned by: myers
mutable description: This mlsR gene was chosen as an additional selection marker for transformation of Gram positive chassis organisms. In the intended chassis organism of Clostridium acetobutylicum, the concentrations for selectivity were known, making this a convenient choice.
discontinued: false
dominant: false
group u list: _1521_1241_
m user id: 4206
owner id: 14319
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The cloning of this part was relatively straight-forward. No special design considerations were required.
star: false
creator: Jeremiah Miller
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1207001_sequence/1

PyrF
type: ComponentDefinition
persistent identity: BBa T9150
display id: BBa_T9150
version: 1
was generated by: iGEM to SBOL conversion
description: orotidine 5
creator: Jessica Harpole
created: 2005-08-22T11:00:00Z
modified: 2015-05-08T01:14:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.
mutable notes: Four silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. The EcoRI site was eliminated at base 39 with a G -> A mutation. PstI sites were eliminated at 240 and and 441 with T -> C mutations, and at 261 with a T -> G mutation. Mutation choices were selected to match codons with similar codon usage.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T
sequence annotation: G
sequence annotation: PyrF
sequence: https://synbiohub.org/public/igem/BBa_T9150_sequence/1

T
type: SequenceAnnotation
persistent identity: annotation1666364
display id: annotation1666364
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1
type: Range
persistent identity: range1666364
display id: range1666364
version: 1
direction: direction/forward
top level: PyrF
start: 261
end: 266
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1666362
display id: annotation1666362
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1
type: Range
persistent identity: range1666362
display id: range1666362
version: 1
direction: direction/forward
top level: PyrF
start: 39
end: 44
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666363
display id: annotation1666363
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1
type: Range
persistent identity: range1666363
display id: range1666363
version: 1
direction: direction/forward
top level: PyrF
start: 240
end: 245
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666373
display id: annotation1666373
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1
type: Range
persistent identity: range1666373
display id: range1666373
version: 1
direction: direction/forward
top level: PyrF
start: 441
end: 446
orientation: inline

PyrF
type: SequenceAnnotation
persistent identity: annotation1892252
display id: annotation1892252
version: 1
top level: PyrF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1
type: Range
persistent identity: range1892252
display id: range1892252
version: 1
direction: direction/forward
top level: PyrF
start: 1
end: 741
orientation: inline

BBa_K1486027
type: ComponentDefinition
persistent identity: BBa K1486027
display id: BBa_K1486027
version: 1
was generated by: iGEM to SBOL conversion
description: R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K801012
component: BBa_K1486019
component: BBa_K1486037
component: BBa_K1486020
component: BBa_K1486024
sequence annotation: BBa_K1486019
sequence annotation: BBa_K801012
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486027_sequence/1

BBa_K801012
type: Component
persistent identity: component2417710
display id: component2417710
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K801012

BBa_K1486019
type: Component
persistent identity: component2417707
display id: component2417707
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486019

BBa_K1486037
type: Component
persistent identity: component2417706
display id: component2417706
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486037

BBa_K1486020
type: Component
persistent identity: component2417708
display id: component2417708
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486020

BBa_K1486024
type: Component
persistent identity: component2417714
display id: component2417714
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486024

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417707
display id: annotation2417707
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
type: Range
persistent identity: range2417707
display id: range2417707
version: 1
top level: BBa_K1486027
direction: direction/reverse
start: 40
end: 369
orientation: inline

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2417710
display id: annotation2417710
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
type: Range
persistent identity: range2417710
display id: range2417710
version: 1
top level: BBa_K1486027
direction: direction/reverse
start: 976
end: 1324
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417706
display id: annotation2417706
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
type: Range
persistent identity: range2417706
display id: range2417706
version: 1
top level: BBa_K1486027
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417714
display id: annotation2417714
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
type: Range
persistent identity: range2417714
display id: range2417714
version: 1
top level: BBa_K1486027
direction: direction/reverse
start: 1325
end: 2681
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2417708
display id: annotation2417708
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
type: Range
persistent identity: range2417708
display id: range2417708
version: 1
top level: BBa_K1486027
direction: direction/reverse
start: 370
end: 975
orientation: inline

BBa_K394000
type: ComponentDefinition
persistent identity: BBa K394000
display id: BBa_K394000
version: 1
was generated by: iGEM to SBOL conversion
description: Resistance Gene for G418 in Yeast
creator: Brian Landry
created: 2010-10-17T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part confers resistance to Geneticin (G418) in yeast. Selection of yeast cells is typically preformed on plates with 200??g/ml of G418.
mutable notes: None
mutable provenance: The part was synthesized from the PMKV005 plasmid used in McCusker et. al. The coding region contains the KanR open reading from transposon Tn903 from E. coli. 1. McCusker JH, Vorachek-Warren MK. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast (Chichester, England). 2004;21(2):163-71. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14755641.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: KanR gene
sequence annotation: HindIII cut site
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K394000_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2104020
display id: annotation2104020
version: 1
top level: BBa_K394000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104020/range2104020/1
type: Range
persistent identity: range2104020
display id: range2104020
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 3
orientation: inline

KanR gene
type: SequenceAnnotation
persistent identity: annotation2104021
display id: annotation2104021
version: 1
top level: BBa_K394000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104021/range2104021/1
type: Range
persistent identity: range2104021
display id: range2104021
version: 1
direction: direction/forward
top level: BBa_K394000
start: 1
end: 810
orientation: inline

HindIII cut site
type: SequenceAnnotation
persistent identity: annotation2104022
display id: annotation2104022
version: 1
top level: BBa_K394000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104022/range2104022/1
type: Range
persistent identity: range2104022
display id: range2104022
version: 1
direction: direction/forward
top level: BBa_K394000
start: 545
end: 549
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2104023
display id: annotation2104023
version: 1
top level: BBa_K394000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1

https://synbiohub.org/public/igem/BBa_K394000/annotation2104023/range2104023/1
type: Range
persistent identity: range2104023
display id: range2104023
version: 1
direction: direction/forward
top level: BBa_K394000
start: 808
end: 813
orientation: inline

BBa_K1486020
type: ComponentDefinition
persistent identity: BBa K1486020
display id: BBa_K1486020
version: 1
was generated by: iGEM to SBOL conversion
description: rLuc[2]
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: to edit
owned by: james
owned by: myers
mutable description: This is the second part of the split Renilla luciferase. To be used with rLuc[1].
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486020_sequence/1

BBa_K1486037
type: ComponentDefinition
persistent identity: BBa K1486037
display id: BBa_K1486037
version: 1
was generated by: iGEM to SBOL conversion
description: 13 amino acids linker [GGGS GGGGS GGGS]
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
mutable provenance: To edit
owned by: james
owned by: myers
mutable description: To edit
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To edit
star: false
creator: EPFL iGem team 2014
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: 13 amino acids linker
sequence: https://synbiohub.org/public/igem/BBa_K1486037_sequence/1

13 amino acids linker
type: SequenceAnnotation
persistent identity: annotation2417686
display id: annotation2417686
version: 1
top level: BBa_K1486037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486037/annotation2417686/range2417686/1

https://synbiohub.org/public/igem/BBa_K1486037/annotation2417686/range2417686/1
type: Range
persistent identity: range2417686
display id: range2417686
version: 1
top level: BBa_K1486037
direction: direction/forward
start: 1
end: 39
orientation: inline

BBa_J133003
type: ComponentDefinition
persistent identity: BBa J133003
display id: BBa_J133003
version: 1
was generated by: iGEM to SBOL conversion
description: aadA gene with lacIQ promoter and RBS and oriT
created: 2011-07-11T11:00:00Z
modified: 2015-08-31T04:08:30Z
owned by: james
owned by: myers
mutable description: BBa_K125530 with an oriT postfixed, intermediate from the 2008 iGEM Hawaii team collection
discontinued: false
dominant: false
group u list: _634_
m user id: 0
owner id: 2680
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Norman Wang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K125530
component: BBa_K125320
sequence annotation: BBa_K125320
sequence annotation: BBa_K125530
sequence: https://synbiohub.org/public/igem/BBa_J133003_sequence/1

BBa_K125530
type: Component
persistent identity: component2123318
display id: component2123318
version: 1
top level: BBa_J133003
access: public
definition: BBa_K125530

BBa_K125320
type: Component
persistent identity: component2123319
display id: component2123319
version: 1
top level: BBa_J133003
access: public
definition: OriT-RP4

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation2123319
display id: annotation2123319
version: 1
top level: BBa_J133003
location: https://synbiohub.org/public/igem/BBa_J133003/annotation2123319/range2123319/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_J133003/annotation2123319/range2123319/1
type: Range
persistent identity: range2123319
display id: range2123319
version: 1
top level: BBa_J133003
direction: direction/reverse
start: 864
end: 962
orientation: inline

BBa_K125530
type: SequenceAnnotation
persistent identity: annotation2123318
display id: annotation2123318
version: 1
top level: BBa_J133003
location: https://synbiohub.org/public/igem/BBa_J133003/annotation2123318/range2123318/1
component: BBa_K125530

https://synbiohub.org/public/igem/BBa_J133003/annotation2123318/range2123318/1
type: Range
persistent identity: range2123318
display id: range2123318
version: 1
top level: BBa_J133003
direction: direction/reverse
start: 1
end: 855
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) backwards
sequence annotation: stop
sequence annotation: Fwd primer J31006
sequence annotation: Rev primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

ptrA
type: ComponentDefinition
persistent identity: BBa K678045
display id: BBa_K678045
version: 1
was generated by: iGEM to SBOL conversion
description: ptrA, selection marker for Aspergillus nidulans
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
mutable provenance: ptrA
owned by: james
owned by: myers
mutable description: ptrA, selection marker for Aspergillus nidulans
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Compatible with the Plug'n'Play assembly standard
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker 5
sequence annotation: ptrA cassette
sequence: https://synbiohub.org/public/igem/BBa_K678045_sequence/1

Linker 5
type: SequenceAnnotation
persistent identity: annotation2127421
display id: annotation2127421
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127421/range2127421/1
type: Range
persistent identity: range2127421
display id: range2127421
version: 1
top level: ptrA
direction: direction/forward
start: 1
end: 8
orientation: inline

ptrA cassette
type: SequenceAnnotation
persistent identity: annotation2127422
display id: annotation2127422
version: 1
top level: ptrA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1

https://synbiohub.org/public/igem/BBa_K678045/annotation2127422/range2127422/1
type: Range
persistent identity: range2127422
display id: range2127422
version: 1
top level: ptrA
direction: direction/forward
start: 9
end: 1899
orientation: inline

https://synbiohub.org/public/igem/BBa_K125520_sequence/1
type: Sequence
persistent identity: BBa K125520 sequence
display id: BBa_K125520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389011_sequence/1
type: Sequence
persistent identity: BBa K389011 sequence
display id: BBa_K389011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486034_sequence/1
type: Sequence
persistent identity: BBa K1486034 sequence
display id: BBa_K1486034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgagatcacatggtcttgttagagttcgtgactgccgctggtataacattaggaatggacgaattgtacaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486036_sequence/1
type: Sequence
persistent identity: BBa K1486036 sequence
display id: BBa_K1486036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctccaaagaagatcatttttgtcggccatgattggggtgcttgtttggcatttcattatagctatgagcatcaagataagatcaaagcaatagttcacgctgaaagtgtagtagatgtgattgaatcatgggatgaatggcctgatattgaagaagatattgcgttgatcaaatctgaagaaggagaaaaaatggttttggagaataacttcttcgtggaaaccatgttgccatcaaaaatcatgagaaagttagaaccagaagaatttgcagcatatcttgaaccattcaaagagaaaggtgaagttcgtcgtccaacattatcatggcctcgtgaaatcccgttagtaaaaggtggtaaacctgacgttgtacaaattgttaggaattataatgcttatctacgtgcaagtgatgatttaccaaaaatgtttattgaatcggacccaggattcttttccaatgctattgttgaaggtgccaagaagtttcctaatactgaatttgtcaaagtaaaaggtcttcatttttcgcaagaagatgcacctgatgaaatgggaaaatatatcaaatcgttcgttgagcgagttctcaaaaatgaacaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgacagtcaacactaagacctatagtgagagagcagaaactcatgcctcaccagtagcacagcgattatttcgattaatggaactgaagaaaaccaatttatgtgcatcaattgacgttgataccactaaggaattccttgaattaattgataaattaggtccttatgtatgcttaatcaagactcatattgatataatcaatgatttttcctatgaatccactattgaaccattattagaactttcacgtaaacatcaatttatgatttttgaagatagaaaatttgctgatattggtaataccgtaaagaaacaatatattggtggagtttataaaattagtagttgggcagatattaccaatgctcatggtgtcactgggaatggagtggttgaaggattaaaacagggagctaaagaaaccaccaccaaccaagagccaagagggttattgatgttagctgaattatcatcagtgggatcattagcatatggagaatattctcaaaaaactgttgaaattgctaaatccgataaggaatttgttattggatttattgcccaacgtgatatgggtggccaagaagaaggatttgattggcttattatgacacctggagttggattagatgataaaggtgatggattaggacaacaatatagaactgttgatgaagttgttagcactggaactgatattatcattgttggtagaggattgtttggtaaaggaagagatccagatattgaaggtaaaaggtatagaaatgctggttggaatgcttatttgaaaaagactggccaattataatcagtactga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486020_sequence/1
type: Sequence
persistent identity: BBa K1486020 sequence
display id: BBa_K1486020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaaagaagatcatttttgtcggccatgattggggtgcttgtttggcatttcattatagctatgagcatcaagataagatcaaagcaatagttcacgctgaaagtgtagtagatgtgattgaatcatgggatgaatggcctgatattgaagaagatattgcgttgatcaaatctgaagaaggagaaaaaatggttttggagaataacttcttcgtggaaaccatgttgccatcaaaaatcatgagaaagttagaaccagaagaatttgcagcatatcttgaaccattcaaagagaaaggtgaagttcgtcgtccaacattatcatggcctcgtgaaatcccgttagtaaaaggtggtaaacctgacgttgtacaaattgttaggaattataatgcttatctacgtgcaagtgatgatttaccaaaaatgtttattgaatcggacccaggattcttttccaatgctattgttgaaggtgccaagaagtttcctaatactgaatttgtcaaagtaaaaggtcttcatttttcgcaagaagatgcacctgatgaaatgggaaaatatatcaaatcgttcgttgagcgagttctcaaaaatgaacaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823020_sequence/1
type: Sequence
persistent identity: BBa K823020 sequence
display id: BBa_K823020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: aactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggctttta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T9000_sequence/1
type: Sequence
persistent identity: BBa T9000 sequence
display id: BBa_T9000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcatacgcggaaggcaataacggaggcgcttcaaaaactcggagtccaaaccggtgacctattgatggtgcatgcctcacttaaagcgattggtccggtcgaaggaggagcggagacggtcgttgccgcgttacgctccgcggttgggccgactggcactgtgatgggatacgcatcgtgggaccgatcaccctacgaggagactcgtaatggcgctcggttggatgacaaaacccgccgtacctggccgccgttcgatcccgcaacggccgggacttaccgtgggttcggcctgctgaatcagtttctggttcaagcccccggcgcgcggcgcagcgcgcaccccgatgcatcgatggtcgcggttggtccactggctgaaacgctgacggagcctcacaagctcggtcacgccttgggggaagggtcgcccgtcgagcggttcgttcgccttggcgggaaggccctgctgttgggtgcgccgctaaactccgttaccgcattgcactacgccgaggcggttgccgatatccccaacaaacggcgggtgacgtatgagatgccgatgcttggaagcaacggcgaagtcgcctggaaaacggcatcggattacgattcaaacggcattctcgattgctttgctatcgaaggaaagccggatgcggtcgaaactatagcaaatgcttacgtgaagctcggtcgccatcgagaaggtgtcgtgggctttgctcagtgctacctgttcgacgcgcaggacatcgtgacgttcggcgtcacctatcttgagaagcatttcggaaccactccgatcgtgccagcacacgaagtcgccgagtgctcttgcgagccttcaggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486026_sequence/1
type: Sequence
persistent identity: BBa K1486026 sequence
display id: BBa_K1486026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagaatggtttatctaaaaatgtaccggcattacatatgggtggtttaggtgacggtgctggtttaattaacatgtctaaaggcgaggaattgtttacaggtgtagttcctattttggtcgaactagatggagatgtcaacggtcataagttttctgtacgtggtgaaggcgaaggtgatgctactaatggcaaactgactctgaagttcatctgtacgaccggaaaattgccagttccatggcctacattagtaacaacattaacctacggagttcaatgcttttcaagatacccagatcacatgaagcagcatgactttttcaaaagtgctatgccagaaggttatgtgcaggagagaactatctccttcaaagatgatggtacttataagacaagagcagaggtaaagtttgaaggcgatacattggttaatagaattgagcttaaaggcattgattttaaggaagatgggaatatcctcggacataaacttgaatacaatttcaattcacataatgtttatatcacagcagataaacaaaagaatggtataaaagcgaactttaagattagacataacgtggaagatggtagcgtccaattagccgaccattaccaacaaaacactcctatcggggacgggccagttctccttccagacaaccactacctatcaacccaatctgttctatctaaagaccctaatgaaaaacgagatcacatggtcttgttagagttcgtgactgccgctggtataacattaggaatggacgaattgtacaaataaggcgcgccacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccggcagatccgctagggataacagggtaatatagatctgtttagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K319041_sequence/1
type: Sequence
persistent identity: BBa K319041 sequence
display id: BBa_K319041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgatgcatacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaccactcttgacgacacggcttaccggtaccgcaccagtgtcccgggggacgccgaggccatcgaggcactggatgggtccttcaccaccgacaccgtcttccgcgtcaccgccaccggggacggcttcaccctgcgggaggtgccggtggacccgcccctgaccaaggtgttccccgacgacgaatcggacgacgaatcggacgacggggaggacggcgacccggactcccggacgttcgtcgcgtacggggacgacggcgacctggcgggcttcgtggtcatctcgtactcggcgtggaaccgccggctgaccgtcgaggacatcgaggtcgccccggagcaccgggggcacggggtcgggcgcgcgttgatggggctcgcgacggagttcgccggcgagcggggcgccgggcacctctggctggaggtcaccaacgtcaacgcaccggcgatccacgcgtaccggcggatggggttcaccctctgcggcctggacaccgccctgtacgacggcaccgcctcggacggcgagcggcaggcgctctacatgagcatgccctgcccctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatactaacgccgccatccagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31002_sequence/1
type: Sequence
persistent identity: BBa J31002 sequence
display id: BBa_J31002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143031_sequence/1
type: Sequence
persistent identity: BBa K143031 sequence
display id: BBa_K143031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1323012_sequence/1
type: Sequence
persistent identity: BBa K1323012 sequence
display id: BBa_K1323012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaatttgattaacggaaaaataccaaatcaagcgattcaaacattaaaaatcgtaaaagatttatttggaagttcaatagttggagtatatctatttggttcagcagtaaatggtggtttacgcattaacagcgatgtagatgttctagtcgtcgtgaatcatagtttacctcaattaactcgaaaaaaactaacagaaagactaatgactatatcaggaaagattggaaatacggattctgttagaccacttgaagttacggttataaataggagtgaagttgtcccttggcaatatcctccaaaaagagaatttatatacggtgagtggctcaggggtgaatttgagaatggacaaattcaggaaccaagctatgatcctgatttggctattgttttagcacaagcaagaaagaatagtatttctctatttggtcctgattcttcaagtatacttgtctccgtacctttgacagatattcgaagagcaattaaggattctttgccagaactaattgaggggataaaaggtgatgagcgtaatgtaattttaaccctagctcgaatgtggcaaacagtgactactggtgaaattacctcgaaagatgtcgcagcagaatgggctatacctcttttacctaaagagcatgtaactttactggatatagctagaaaaggctatcggggagagtgtgatgataagtgggaaggactatattcaaaggtgaaagcactcgttaagtatatgaaaaattctatagaaacttctctcaattag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678021_sequence/1
type: Sequence
persistent identity: BBa K678021 sequence
display id: BBa_K678021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgtccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486030_sequence/1
type: Sequence
persistent identity: BBa K1486030 sequence
display id: BBa_K1486030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctatggcttcgaaagtttatgatccagaacaaaggaaacggatgataactggtccgcagtggtgggccagatgtaaacaaatgaatgttcttgattcatttattaattattatgattcagaaaaacatgcagaaaatgctgttatttttttacatggtaacgcggcctcttcttatttatggcgacatgttgtgccacatattgagccagtagcgcggtgtattataccagaccttattggtatgggcaaatcaggcaaatctggtaatggttcttataggttacttgatcattacaaatatcttactgcatggtttgaacttcttaatttagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389002_sequence/1
type: Sequence
persistent identity: BBa K389002 sequence
display id: BBa_K389002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678047_sequence/1
type: Sequence
persistent identity: BBa K678047 sequence
display id: BBa_K678047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgtaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagcgctgcgatgataccgcgagaaccacgctcaccggctccggatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatcgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486025_sequence/1
type: Sequence
persistent identity: BBa K1486025 sequence
display id: BBa_K1486025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678044_sequence/1
type: Sequence
persistent identity: BBa K678044 sequence
display id: BBa_K678044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgttaagctccctaattggcccatccggcatctgtagggcgtccaaatatcgtgcctctcctgctttgcccggtgtatgaaaccggaaaggccgctcaggagctggccagcggcgcagaccgggaacacaagctggcagtcgacccatccggtgctctgcactcgacctgctgaggtccctcagtccctggtaggcagctttgccccgtctgtccgcccggtgtgtcggcggggttgacaaggtcgttgcgtcagtccaacatttgttgccatattttcctgctctccccaccagctgctcttttcttttctctttcttttcccatcttcagtatattcatcttcccatccaagaacctttatttcccctaagtaagtactttgctacatccatactccatccttcccatcccttattcctttgaacctttcagttcgagctttcccacttcatcgcagcttgactaacagctaccccgcttgagcagacatcaccatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactgaccgacgccgaccaacaccgccggtccgacggcggcccacgggtcccaggagcttgagatccacttaacgttactgaaatcatcaaacagcttgacgaatctggatataagatcgttggtgtcgatgtcagctccggagttgagacaaatggtgttcaggatctcgataagatacgttcatttgtccaagcagcaaagagtgccttctagtgatttaatagctccatgtcaacaagaataaaacgcgttttcgggtttacctcttccagatacagctcatctgcaatgcattaatgcattgactgcaacctagtaacgcctttcaggctccggcgaagagaagaatagcttagcagagctattttcattttcgggagacgagatcaagcagatcaacggtcgtcaagagacctacgagactgaggaatccgctcttggctccacgcgactatatatttgtctctaattgtactttgacatgctcctcttctttactctgatagcttgactatgaaaattccgtcaccagctcctgggttcgcaaagataattgcatgtttcttccttgaactctcaagcctacaggacacacattcatcgtaggtataaacctcgaaatcatttcctactaagatggtatacaatagtaaccatgcatggttgcctagtgaatgctccgtaacacccaatacgccggccgaaacttttttacaactctcctatgagtcgtttacccagaatgcacaggtacacttgtttagaggtaatccttctttctagagcttggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486029_sequence/1
type: Sequence
persistent identity: BBa K1486029 sequence
display id: BBa_K1486029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctatgtctaaaggcgaggaattgtttacaggtgtagttcctattttggtcgaactagatggagatgtcaacggtcataagttttctgtacgtggtgaaggcgaaggtgatgctactaatggcaaactgactctgaagttcatctgtacgaccggaaaattgccagttccatggcctacattagtaacaacattaacctacggagttcaatgcttttcaagatacccagatcacatgaagcagcatgactttttcaaaagtgctatgccagaaggttatgtgcaggagagaactatctccttcaaagatgatggtacttataagacaagagcagaggtaaagtttgaaggcgatacattggttaatagaattgagcttaaaggcattgattttaaggaagatgggaatatcctcggacataaacttgaatacaatttcaattcacataatgtttatatcacagcagataaacaaaagaatggtataaaagcgaactttaagattagacataacgtggaagatggtagcgtccaattagccgaccattaccaacaaaacactcctatcggggacgggccagttctccttccagacaaccactacctatcaacccaatctgttctatctaaagaccctaatgaaaaagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1323011_sequence/1
type: Sequence
persistent identity: BBa K1323011 sequence
display id: BBa_K1323011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgagaaaaatataaaacacagtcaaaactttattacttcaaaacataatatagataaaataatgacaaatataagattaaatgaacatgataatatctttgaaatcggctcaggaaaagggcattttacccttgaattagtacagaggtgtaatttcgtaactgccattgaaatagaccataaattatgcaaaactacagaaaataaacttgttgatcacgataatttccaagttttaaacaaggatatattgcagtttaaatttcctaaaaaccaatcctataaaatatttggtaatataccttataacataagtacggatataatacgcaaaattgtttttgatagtatagctgatgagatttatttaatcgtggaatacgggtttgctaaaagattattaaatacaaaacgctcattcgcattatttttaatggcagaagttgatatttctatattaagtatggttccaagagaatattttcatcctaaacctaaagtgaatagctcacttatcagattaaatagaaaaaaatcaagaatatcacacaaagataaacagaagtataattatttcgttatgaaatgggttaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125530_sequence/1
type: Sequence
persistent identity: BBa K125530 sequence
display id: BBa_K125530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1207001_sequence/1
type: Sequence
persistent identity: BBa K1207001 sequence
display id: BBa_K1207001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacgagaaaaatataaaacacagtcaaaactttattacttcaaaacataatatagataaaataatgacaaatataagattaaatgaacatgataatatctttgaaatcggctcaggaaaagggcattttacccttgaattagtacagaggtgtaatttcgtaactgccattgaaatagaccataaattatgcaaaactacagaaaataaacttgttgatcacgataatttccaagttttaaacaaggatatattgcagtttaaatttcctaaaaaccaatcctataaaatatttggtaatataccttataacataagtacggatataatacgcaaaattgtttttgatagtatagctgatgagatttatttaatcgtggaatacgggtttgctaaaagattattaaatacaaaacgctcattggcattatttttaatggcagaagttgatatttctatattaagtatggttccaagagaatattttcatcctaaacctaaagtgaatagctcacttatcagattaaatagaaaaaaatcaagaatatcacacaaagataaacagaagtataattatttcgttatgaaatgggttaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31004_sequence/1
type: Sequence
persistent identity: BBa J31004 sequence
display id: BBa_J31004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtttgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgcttcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagtgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgctttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaatgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015010_sequence/1
type: Sequence
persistent identity: BBa K1015010 sequence
display id: BBa_K1015010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcgaaaataatagagggaaaatcagtttttgatatcaaaattatacatgtcaacgataatacaaaatataatacaaaatataagatgttatcagtatttattatgcatttagaatattaaaattccttcattacactcttggcggtttcacttatcaacttatcatttggcttatcacttttattgtctttattcgtaaaaatgactaaaacaataggttcagattggcccttaggataaacaaaagcaacatcatttctagaagcatatgttattgcttgaccacttttatcagcaaccttatagtcttttggaacaccgtctttaattaaagtatctccgcttttattatttaacattaaatcaagtaagaattttttgttttctttgcttaattttccatttgcgataagtttatttaaagtcttaccgaaagcagcaggtgttgaagtatcttttttgctctttggtgaatagtaatttaattctatctcatatctaactggatttgttactttatctcctagttcttttagacgttgtttaacttttttgattccaccgatttcttttataattttattgtttgctgtattatcactatatgtcattgaagcctcaataagtgcttttaaagtgatatcttttcctacatatttttctaaaataggagaataagcaactatatcatctttgttaatatgtacttttttatttaacttattataaggtacttgttctaacaaaatagcactatttatcgcttttgaagttgaagcataggcaaatctcttatctgaattaaattttacttccttaccacttttagtatctaaagcataaacaccaatatgagcattatattttttttctaaatcatttaactctttggcatgtgaactgtttgaattacatgcacttaaaactaaagcaattacaattaaaaatattaactttttcaaaataaaccctccgatatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125320_sequence/1
type: Sequence
persistent identity: BBa K125320 sequence
display id: BBa_K125320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678022_sequence/1
type: Sequence
persistent identity: BBa K678022 sequence
display id: BBa_K678022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgtctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930002_sequence/1
type: Sequence
persistent identity: BBa K1930002 sequence
display id: BBa_K1930002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaagggcaaggtgtcaattctcatgtttgacagcttatcatcggcaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgacatggatgagcgatgatgatatccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagacgacaggcgtttaaggatggagatcaattcacaatcggaaaaggagatccaatttggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486028_sequence/1
type: Sequence
persistent identity: BBa K1486028 sequence
display id: BBa_K1486028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctaaaggcgaggaattgtttacaggtgtagttcctattttggtcgaactagatggagatgtcaacggtcataagttttctgtacgtggtgaaggcgaaggtgatgctactaatggcaaactgactctgaagttcatctgtacgaccggaaaattgccagttccatggcctacattagtaacaacattaacctacggagttcaatgcttttcaagatacccagatcacatgaagcagcatgactttttcaaaagtgctatgccagaaggttatgtgcaggagagaactatctccttcaaagatgatggtacttataagacaagagcagaggtaaagtttgaaggcgatacattggttaatagaattgagcttaaaggcattgattttaaggaagatgggaatatcctcggacataaacttgaatacaatttcaattcacataatgtttatatcacagcagataaacaaaagaatggtataaaagcgaactttaagattagacataacgtggaagatggtagcgtccaattagccgaccattaccaacaaaacactcctatcggggacgggccagttctccttccagacaaccactacctatcaacccaatctgttctatctaaagaccctaatgaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930003_sequence/1
type: Sequence
persistent identity: BBa K1930003 sequence
display id: BBa_K1930003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaccaagcctgcaaaaacaaagtgcaaagatatcagtaaggtcttaaaggccgaagcggtggcctaagataaaactgggcgccctggcgttagttccgcagaagataaaactgggctaaggcatcaaagaaggtatctaaatccgaagtagttagctaagtgattggcgggcgaaggaatttgctaagctatgaaggattggactaagccattcccgaagctatcacataagcgatgacatgcaccgcagcagacaggtacaaataagaaaactttgaagtctttaagaattagtattaagaagtgatgaagggctatgccaccctcctagagacagctataagctcttaaggagtgaagctgttgaataaggcgtagtagaagcaattaagtcctgaagttctgaagctgttgacgaagataaaactctgaataataagacctactataagtgctgaagcgttgaagagataagacagagaagtcttgaagtgcttaaagaagccttcaactaagtgattacactgctaagagatgaagcattgcacccaccgactccggcaaaaaagggcaaggtgtcaattctcatgtttgacagcttatcatcggcaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgacatggatgagcgatgatgatatccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1033990_sequence/1
type: Sequence
persistent identity: BBa K1033990 sequence
display id: BBa_K1033990_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgagtttattcttgacacagatatttatgatataataactgagtacagcttaacataaggaggaaaaacagatggcgaaactgacctcggcggttccggttctgacggcacgtgatgtggcgggcgcggttgaattttggacggatcgtctgggcttcagtcgtgattttgtggaagatgacttcgcaggcgtggttcgcgatgacgtcaccctgtttatttccgcagttcaggatcaagtcgtgccggacaacacgctggcttgggtgtgggttcgtggcctggatgaactgtatgcggaatggagcgaagttgtctctaccaatttccgtgacgcgagcggtccggccatgacggaaatcggcgaacagccgtggggtcgcgaatttgctctgcgtgacccggctggcaactgtgtccatttcgtggctgaagaacaagattgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J133653_sequence/1
type: Sequence
persistent identity: BBa J133653 sequence
display id: BBa_J133653_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagctaattagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgtgattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataattagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J133003_sequence/1
type: Sequence
persistent identity: BBa J133003 sequence
display id: BBa_J133003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataatactagaggaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930004_sequence/1
type: Sequence
persistent identity: BBa K1930004 sequence
display id: BBa_K1930004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataggtgaaaatgtgaacattctgtggagacgtaaagtataaaaagttttttaactttaaacagattgacacatgttaggggctattgtatgctaaacgagggtattatgagaaggttttcatagctttcattatagtcctcatcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttcgaacaggcccgttttgccggtcaaaaaaggtcttctactaagggttaaccgctgatttttgccgtatccaagctatacattattttttcatcaggagacagataattgatggaaacctacaatcatacatatcggcaccacaacttttcacataaagacttaagtgatctcaccttcaccgcttgcacattcattcgcagcgactttcgacgtgctaacttgcgtgatacgacattcgtcaactgcaagttcattgaacagggtgatatcgaaggctgccactttgatgtcgcagatcttcgtgatgcaagtttccaacaatgccaacttgcgatggcaaacttcagtaatgccaattgctacggtatagagttccgtgcgtgtgatttaaaaggtgccaacttttcccgaacaaactttgcccatcaagtgagtaatcgtatgtacttttgctcagcatttatttctggatgtaatctttcctatgccaatatggagagggtttgtttagaaaaatgtgagttgtttgaaaatcgctggataggaacgaacctagcgggtgcatcactgaaagagtcagacttaagtcgaggtgttttttccgaagatgtctgggggcaatttagcctacagggtgccaatttatgccacgccgaactcgacggtttagatccccgcaaagtcgatacatcaggtatcaaaattgcagcctggcagcaagaactgattctcgaagcactgggtattgttgtttatcctgactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31005_sequence/1
type: Sequence
persistent identity: BBa J31005 sequence
display id: BBa_J31005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801012_sequence/1
type: Sequence
persistent identity: BBa K801012 sequence
display id: BBa_K801012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1073000_sequence/1
type: Sequence
persistent identity: BBa K1073000 sequence
display id: BBa_K1073000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K320004_sequence/1
type: Sequence
persistent identity: BBa K320004 sequence
display id: BBa_K320004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagaccttgggcaaggccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtacgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcaccctaccgcatggagataagcatggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcagccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgcgcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccagactatcggctgtagccgcctcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaattttactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486037_sequence/1
type: Sequence
persistent identity: BBa K1486037 sequence
display id: BBa_K1486037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486024_sequence/1
type: Sequence
persistent identity: BBa K1486024 sequence
display id: BBa_K1486024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914009_sequence/1
type: Sequence
persistent identity: BBa K914009 sequence
display id: BBa_K914009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678042_sequence/1
type: Sequence
persistent identity: BBa K678042 sequence
display id: BBa_K678042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgtgctagtggaggtcaacacatcaatgctattttggtttagtcgtccaggcggatcacaaaatttgtgtcgtttgacaagatggttcatttaggcaactggtcagatcagcccacttgtaagcagtagcggcggcgctcgaagtgtgactcttattagcagacaggaacgaggacattattatcatctgctgcttggtgcacgataacttgtgcgtttgtcaagcaaggtaagtgaacgacccggtcataccttcttaagttcgcccttcctccctttatttcagattcaatctgacttacctattctacccaagcatccaaatgcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggagttcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctggagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcgcctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagagtagatgccgaccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23012_sequence/1
type: Sequence
persistent identity: BBa J23012 sequence
display id: BBa_J23012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccaagcgagctcgatatccgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgtacgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389003_sequence/1
type: Sequence
persistent identity: BBa K389003 sequence
display id: BBa_K389003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486019_sequence/1
type: Sequence
persistent identity: BBa K1486019 sequence
display id: BBa_K1486019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcgaaagtttatgatccagaacaaaggaaacggatgataactggtccgcagtggtgggccagatgtaaacaaatgaatgttcttgattcatttattaattattatgattcagaaaaacatgcagaaaatgctgttatttttttacatggtaacgcggcctcttcttatttatggcgacatgttgtgccacatattgagccagtagcgcggtgtattataccagaccttattggtatgggcaaatcaggcaaatctggtaatggttcttataggttacttgatcattacaaatatcttactgcatggtttgaacttcttaattta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1323013_sequence/1
type: Sequence
persistent identity: BBa K1323013 sequence
display id: BBa_K1323013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagattgttcatgaaattaaggaacgaatattggataaatatggggatgatgttaaggctattggtgtttatggctctcttggtcgtcagactgatgggccctattcggatattgagatgatgtgtgtcatgtcaacagaggaagcagagttcagccatgaatggacaaccggtgagtggaaggtggaagtgaattttgatagcgaagagattctactagattatgcatctcaggtggaatcagattggccgcttacacatggtcaatttttctctattttgccgatttatgattcaggtggatacttagagaaagtgtatcaaactgctaaatcggtagaagcccaaacgttccacgatgcgatttgtgcccttatcgtagaagagctgtttgaatatgcaggcaaatggcgtaatattcgtgtgcaaggaccgacaacatttctaccatccttgactgtacaggtagcaatggcaggtgccatgttgattggtctgcatcatcgcatctgttatacgacgagcgcttcggtcttaactgaagcagttaagcaatcagatcttccttcaggttatgaccatctgtgccagttcgtaatgtctggtcaactttccgactctgagaaacttctggaatcgctagagaatttctggaatgggattcaggagtggacagaacgacacggatatatagtggatgtgtcaaaacgcataccattttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678045_sequence/1
type: Sequence
persistent identity: BBa K678045 sequence
display id: BBa_K678045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acttgcgtggagatcgtccgccgatgtcaggatgatttcacttgtttcttgtccggctcaccggtcaaagctaaagaggagcaaaaggaacggatagaatcgggtgccgctgatctatacggtatagtgcccttatcacgttgactcaacccatgctatttaactcaacccctccttctgaaccccaccatcttcttccttttcctctcatcccacacaattctctatctcagatttgaattccaaaagtcctcggacgaaactgaacaagtcttcctcccttcgataaacctttggtgattggaataactgaccatcttctatagttcccaaaccaaccgacaatgtaaatacactcctcgattagccctctagagggcatacgatggaagtcatggaatacttttggctggactctcacaatgatcaaggtatcttaggtaacgtctttggcgtgggccggtgttcgttcccagtcatcgatgcattcacatgccctccctaagctgggccctagactctaggatcctagtctagaaggacatggcatcgatggactgggttcgttctgagattatacggctaaaacttgatctggataataccagcgaaaagggtcatgccttctctcgttcttcctgttgatggaatggctaacagatgatagtcattgcaacttgaaacatgtctcctccagctgccatctacgaacccactgtggccgctaccggcctcaagggtaaggtcgtggtttctgagaccgtccccgttgagggagcttctcagaccaagctgttggaccatttcggtggcaagtgggacgagttcaagttcgcccctatccgcgaaagccaggtctctcgtgccatgaccagacgttactttgaggacctggacaagtacgctgaaagtgacgttgtcattgttggtgctggttcctgcggtctgagcactgcgtacgtcttggccaaggctcgtccggacctgaagattgctatcgtcgaggccagcgtctctcctggtcagtagtccatgatggattgccttgcactcagctttccggaactaacgtgcaataggtggcggtgcctggttgggtggccaactcttttctgctatggtcatgcgccgtcccgcggaagtcttcctgaacgagctgggtgttccttacgaagaggacgcaaaccccaactacgttgtcgtcaagcacgcctccctgtttacctcgacactcatgtcgaaggttctctccttccccaatgtcaagctcttcaatgctaccgctgttgaggacttgatcacccgtccgaccgagaacggcaacccccagattgctggtgttgtcgtcaactggacgctggtcacccttcaccacgatgatcactcctgcatggaccccaacactatcaacgctcctgtcatcatcagtaccactggtcacgatgggccattcggcgccttctgtgcgaagcgcttggtgtccatgggcagcgtcgacaagctaggtggcatgcgtggtctcgacatgaactcggccgaggatgccatcgtcaagaacacccgcgaggttactaagggcttgataatcggcggtatggagctgtctgaaattgatggctttaaccgcatgggccctaccttcggtgccatggttctcagtggtgtcaaggctgccgaggaggcattgaaggtgttcgacgagcgtcagcgcgagtgtgctgagtaaatgactcactacccgaatgggttcagtgcatgaaccggatttgtcttacggtctttgacgataggggaatgatgattatgtgatagttctgagatttgaatgaactcgttagctcgtaatccacatgcatatgtaaatggctgtgtcccgtatgtaacggtggggcattctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T9010_sequence/1
type: Sequence
persistent identity: BBa T9010 sequence
display id: BBa_T9010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcatacgcggaaggcaataacggaggcgcttcaaaaactcggagtccaaaccggtgacctattgatggtgcatgcctcacttaaagcgattggtccggtcgaaggaggagcggagacggtcgttgccgcgttacgctccgcggttgggccgactggcactgtgatgggatacgcatcgtgggaccgatcaccctacgaggagactcgtaatggcgctcggttggatgacaaaacccgccgtacctggccgccgttcgatcccgcaacggccgggacttaccgtgggttcggcctgctgaatcagtttctggttcaagcccccggcgcgcggcgcagcgcgcaccccgatgcatcgatggtcgcggttggtccactggctgaaacgctgacggagcctcacaagctcggtcacgccttgggggaagggtcgcccgtcgagcggttcgttcgccttggcgggaaggccctgctgttgggtgcgccgctaaactccgttaccgcattgcactacgccgaggcggttgccgatatccccaacaaacggcgggtgacgtatgagatgccgatgcttggaagcaacggcgaagtcgcctggaaaacggcatcggattacgattcaaacggcattctcgattgctttgctatcgaaggaaagccggatgcggtcgaaactatagcaaatgcttacgtgaagctcggtcgccatcgagaaggtgtcgtgggctttgctcagtgctacctgttcgacgcgcaggacatcgtgacgttcggcgtcacctatcttgagaagcatttcggaaccactccgatcgtgccagcacacgaagtcgccgagtgctcttgcgagccttcaggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1071001_sequence/1
type: Sequence
persistent identity: BBa K1071001 sequence
display id: BBa_K1071001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486033_sequence/1
type: Sequence
persistent identity: BBa K1486033 sequence
display id: BBa_K1486033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctatggcttcgaaagtttatgatccagaacaaaggaaacggatgataactggtccgcagtggtgggccagatgtaaacaaatgaatgttcttgattcatttattaattattatgattcagaaaaacatgcagaaaatgctgttatttttttacatggtaacgcggcctcttcttatttatggcgacatgttgtgccacatattgagccagtagcgcggtgtattataccagaccttattggtatgggcaaatcaggcaaatctggtaatggttcttataggttacttgatcattacaaatatcttactgcatggtttgaacttcttaatttaccaaagaagatcatttttgtcggccatgattggggtgcttgtttggcatttcattatagctatgagcatcaagataagatcaaagcaatagttcacgctgaaagtgtagtagatgtgattgaatcatgggatgaatggcctgatattgaagaagatattgcgttgatcaaatctgaagaaggagaaaaaatggttttggagaataacttcttcgtggaaaccatgttgccatcaaaaatcatgagaaagttagaaccagaagaatttgcagcatatcttgaaccattcaaagagaaaggtgaagttcgtcgtccaacattatcatggcctcgtgaaatcccgttagtaaaaggtggtaaacctgacgttgtacaaattgttaggaattataatgcttatctacgtgcaagtgatgatttaccaaaaatgtttattgaatcggacccaggattcttttccaatgctattgttgaaggtgccaagaagtttcctaatactgaatttgtcaaagtaaaaggtcttcatttttcgcaagaagatgcacctgatgaaatgggaaaatatatcaaatcgttcgttgagcgagttctcaaaaatgaacaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgacagtcaacactaagacctatagtgagagagcagaaactcatgcctcaccagtagcacagcgattatttcgattaatggaactgaagaaaaccaatttatgtgcatcaattgacgttgataccactaaggaattccttgaattaattgataaattaggtccttatgtatgcttaatcaagactcatattgatataatcaatgatttttcctatgaatccactattgaaccattattagaactttcacgtaaacatcaatttatgatttttgaagatagaaaatttgctgatattggtaataccgtaaagaaacaatatattggtggagtttataaaattagtagttgggcagatattaccaatgctcatggtgtcactgggaatggagtggttgaaggattaaaacagggagctaaagaaaccaccaccaaccaagagccaagagggttattgatgttagctgaattatcatcagtgggatcattagcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486031_sequence/1
type: Sequence
persistent identity: BBa K1486031 sequence
display id: BBa_K1486031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgacagtcaacactaagacctatagtgagagagcagaaactcatgcctcaccagtagcacagcgattatttcgattaatggaactgaagaaaaccaatttatgtgcatcaattgacgttgataccactaaggaattccttgaattaattgataaattaggtccttatgtatgcttaatcaagactcatattgatataatcaatgatttttcctatgaatccactattgaaccattattagaactttcacgtaaacatcaatttatgatttttgaagatagaaaatttgctgatattggtaataccgtaaagaaacaatatattggtggagtttataaaattagtagttgggcagatattaccaatgctcatggtgtcactgggaatggagtggttgaaggattaaaacagggagctaaagaaaccaccaccaaccaagagccaagagggttattgatgttagctgaattatcatcagtgggatcattagcatatggagaatattctcaaaaaactgttgaaattgctaaatccgataaggaatttgttattggatttattgcccaacgtgatatgggtggccaagaagaaggatttgattggcttattatgacacctggagttggattagatgataaaggtgatggattaggacaacaatatagaactgttgatgaagttgttagcactggaactgatattatcattgttggtagaggattgtttggtaaaggaagagatccagatattgaaggtaaaaggtatagaaatgctggttggaatgcttatttgaaaaagactggccaattataatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1930007_sequence/1
type: Sequence
persistent identity: BBa K1930007 sequence
display id: BBa_K1930007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataggtgaaaatgtgaacattctgtggagacgtaaagtataaaaagttttttaactttaaacagattgacacatgttaggggctattgtatgctaaacgagggtattatgagaaggttttcatagctttcattatagtcctcatcctcaatgtaaatcctctcagcaaacccgtattatgaggatttatttaagcgaatgaaacagcatccctgcaaggcttgcggatgaatgattttttcgaacaggcccgttttgccggtcaaaaaaggtcttctactaagggttaaccgctgatttttgccgtatccaagctatacattattttttcatcaggagacagataattgatggaaacctacaatcatacatatcggcaccacaacttttcacataaagacttaagtgatctcaccttcaccgcttgcacattcattcgcagcgactttcgacgtgctaacttgcgtgatacgacattcgtcaactgcaagttcattgaacagggtgatatcgaaggctgccactttgatgtcgcagatcttcgtgatgcaagtttccaacaatgccaacttgcgatggcaaacttcagtaatgccaattgctacggtatagagttccgtgcgtgtgatttaaaaggtgccaacttttcccgaacaaactttgcccatcaagtgagtaatcgtatgtacttttgctcagcatttatttctggatgtaatctttcctatgccaatatggagagggtttgtttagaaaaatgtgagttgtttgaaaatcgctggataggaacgaacctagcgggtgcatcactgaaagagtcagacttaagtcgaggtgttttttccgaagatgtctgggggcaatttagcctacagggtgccaatttatgccacgccgaactcgacggtttagatccccgcaaagtcgatacatcaggtatcaaaattgcagcctggcagcaagaactgattctcgaagcactgggtattgttgtttatcctgactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatccggcaaaaaagggcaaggtgtcaattctcatgtttgacagcttatcatcggcaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486035_sequence/1
type: Sequence
persistent identity: BBa K1486035 sequence
display id: BBa_K1486035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctcgagatcacatggtcttgttagagttcgtgactgccgctggtataacattaggaatggacgaattgtacaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagtatgaggtcgctcttattgaccacacctctaccgggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgacagtcaacactaagacctatagtgagagagcagaaactcatgcctcaccagtagcacagcgattatttcgattaatggaactgaagaaaaccaatttatgtgcatcaattgacgttgataccactaaggaattccttgaattaattgataaattaggtccttatgtatgcttaatcaagactcatattgatataatcaatgatttttcctatgaatccactattgaaccattattagaactttcacgtaaacatcaatttatgatttttgaagatagaaaatttgctgatattggtaataccgtaaagaaacaatatattggtggagtttataaaattagtagttgggcagatattaccaatgctcatggtgtcactgggaatggagtggttgaaggattaaaacagggagctaaagaaaccaccaccaaccaagagccaagagggttattgatgttagctgaattatcatcagtgggatcattagcatatggagaatattctcaaaaaactgttgaaattgctaaatccgataaggaatttgttattggatttattgcccaacgtgatatgggtggccaagaagaaggatttgattggcttattatgacacctggagttggattagatgataaaggtgatggattaggacaacaatatagaactgttgatgaagttgttagcactggaactgatattatcattgttggtagaggattgtttggtaaaggaagagatccagatattgaaggtaaaaggtatagaaatgctggttggaatgcttatttgaaaaagactggccaattataatcagtactgacaataaaaagattcttgttttcaagaacttgtcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K156011_sequence/1
type: Sequence
persistent identity: BBa K156011 sequence
display id: BBa_K156011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015002_sequence/1
type: Sequence
persistent identity: BBa K1015002 sequence
display id: BBa_K1015002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1486027_sequence/1
type: Sequence
persistent identity: BBa K1486027 sequence
display id: BBa_K1486027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggctctggaggtggtgggtccggcggtggctctatggcttcgaaagtttatgatccagaacaaaggaaacggatgataactggtccgcagtggtgggccagatgtaaacaaatgaatgttcttgattcatttattaattattatgattcagaaaaacatgcagaaaatgctgttatttttttacatggtaacgcggcctcttcttatttatggcgacatgttgtgccacatattgagccagtagcgcggtgtattataccagaccttattggtatgggcaaatcaggcaaatctggtaatggttcttataggttacttgatcattacaaatatcttactgcatggtttgaacttcttaatttaccaaagaagatcatttttgtcggccatgattggggtgcttgtttggcatttcattatagctatgagcatcaagataagatcaaagcaatagttcacgctgaaagtgtagtagatgtgattgaatcatgggatgaatggcctgatattgaagaagatattgcgttgatcaaatctgaagaaggagaaaaaatggttttggagaataacttcttcgtggaaaccatgttgccatcaaaaatcatgagaaagttagaaccagaagaatttgcagcatatcttgaaccattcaaagagaaaggtgaagttcgtcgtccaacattatcatggcctcgtgaaatcccgttagtaaaaggtggtaaacctgacgttgtacaaattgttaggaattataatgcttatctacgtgcaagtgatgatttaccaaaaatgtttattgaatcggacccaggattcttttccaatgctattgttgaaggtgccaagaagtttcctaatactgaatttgtcaaagtaaaaggtcttcatttttcgcaagaagatgcacctgatgaaatgggaaaatatatcaaatcgttcgttgagcgagttctcaaaaatgaacaataaagctttggacttcttcgccagaggtttggtcaagtctccaatcaaggttgtcggcttgtctaccttgccagaaatttacgaaaagatggaaaagggtcaaatcgttggtagatacgttgttgacacttctaaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggccggtcgaaattcccctaccctatggacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015009_sequence/1
type: Sequence
persistent identity: BBa K1015009 sequence
display id: BBa_K1015009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcgtattaatttcgataagccagatcaagcttgtcgacaattcgaagttcctatactttctagagaataggaacttcggatcaattcatcgatgctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcacccgacgcactttgcgccgaataaatacctgtgacggaagatcacttcgcagaataaataaatcctggtgtccctgttgataccgggaagccctgggccaacttttggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgaaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttttttaaggcagttattggtgcctcactgattaagcattgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31003_sequence/1
type: Sequence
persistent identity: BBa J31003 sequence
display id: BBa_J31003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1021003_sequence/1
type: Sequence
persistent identity: BBa K1021003 sequence
display id: BBa_K1021003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcccagaacgacgcccggccgacatccgccgtgccaccgaggcggacatgccggcggtctgcaccatcgtcaaccactacatcgagacaagcacggtcaacttccgtaccgagccgcaggaaccgcaggagtggacggacgacctcgtccgtctgcgggagcgctatccctggctcgtcgccgaggtggacggcgaggtcgccggcatcgcctacgcgggcccctggaaggcacgcaacgcctacgactggacggccgagtcgaccgtgtacgtctccccccgccaccagcggacgggactgggctccacgctctacacccacctgctgaagtccctggaggcacagggcttcaagagcgtggtcgctgtcatcgggctgcccaacgacccgagcgtgcgcatgcacgaggcgctcggatatgccccccgcggcatgctgcgggcggccggcttcaagcacgggaactggcatgacgtgggtttctggcagctggacttcagcctgccggtaccgccccgtccggtcctgcccgtcaccgagatctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389005_sequence/1
type: Sequence
persistent identity: BBa K389005 sequence
display id: BBa_K389005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1313004_sequence/1
type: Sequence
persistent identity: BBa K1313004 sequence
display id: BBa_K1313004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctaataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914018_sequence/1
type: Sequence
persistent identity: BBa K914018 sequence
display id: BBa_K914018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatagcgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z