//cds/reporter/chromoprotein
type: Collection
persistent identity: cds reporter chromoprotein
display id: cds_reporter_chromoprotein
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1484008
member: BBa_K1680017
member: BBa_K1943003
member: eforRed
member: BBa_K1399002
member: BBa_K1033933
member: BBa_J04450
member: BBa_K1680019
member: BBa_K1484005
member: BBa_K1886005
member: BBa_K1484001
member: BBa_K1680023
member: BBa_K1943001
member: BBa_K1484006
member: BBa_K1484000
member: BBa_K1399000
member: BBa_K1680024
member: BBa_K1886004
member: BBa_K1484007
member: gfasPurple
member: BBa_K1943000
member: tsPurple
member: BBa_K1824019
member: fwYellow
member: BBa_K1467201
member: BBa_K2078002
member: aeBlue
member: BBa_K1680020
member: BBa_K1484004
member: BBa_K1824017
member: meffBlue
member: BBa_K1033932
member: BBa_K1680022
member: spisPink
member: BBa_K1680018
member: amilCP
member: BBa_K1943007
member: BBa_K1033916
member: BBa_K1399001
member: BBa_K1680006
member: BBa_K1943002
member: BBa_K1943006
member: BBa_K1122007
member: BBa_K1744001
member: amilGFP
member: BBa_K1484002
member: BBa_K1023005
member: BBa_K1362461
member: BBa_K1943005
member: cjBlue
member: BBa_K1943004
member: BBa_K1680021
member: BBa_K1484003
member: BBa_K1685005
member: BBa_K1399003

BBa_K1943006
type: ComponentDefinition
persistent identity: BBa K1943006
display id: BBa_K1943006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:40:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943006 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K880005
component: BBa_K1033932
component: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_K1033932
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943006_sequence/1

BBa_K880005
type: Component
persistent identity: component2512750
display id: component2512750
version: 1
top level: BBa_K1943006
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_K1033932
type: Component
persistent identity: component2512752
display id: component2512752
version: 1
top level: BBa_K1943006
access: public
definition: BBa_K1033932

BBa_B0015
type: Component
persistent identity: component2512759
display id: component2512759
version: 1
top level: BBa_K1943006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512750
display id: annotation2512750
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512750/range2512750/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512750/range2512750/1
type: Range
persistent identity: range2512750
display id: range2512750
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 1
end: 55
orientation: inline

BBa_K1033932
type: SequenceAnnotation
persistent identity: annotation2512752
display id: annotation2512752
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512752/range2512752/1
component: BBa_K1033932

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512752/range2512752/1
type: Range
persistent identity: range2512752
display id: range2512752
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 62
end: 739
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512759
display id: annotation2512759
version: 1
top level: BBa_K1943006
location: https://synbiohub.org/public/igem/BBa_K1943006/annotation2512759/range2512759/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943006/annotation2512759/range2512759/1
type: Range
persistent identity: range2512759
display id: range2512759
version: 1
direction: direction/reverse
top level: BBa_K1943006
start: 748
end: 876
orientation: inline

amilCP
type: ComponentDefinition
persistent identity: BBa K592009
display id: BBa_K592009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP, blue chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilCP
sequence: https://synbiohub.org/public/igem/BBa_K592009_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2131628
display id: annotation2131628
version: 1
top level: amilCP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1

https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
type: Range
persistent identity: range2131628
display id: range2131628
version: 1
direction: direction/forward
top level: amilCP
start: 1
end: 666
orientation: inline

tsPurple
type: ComponentDefinition
persistent identity: BBa K1033905
display id: BBa_K1033905
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: tsPurple
sequence annotation: BBa_K1033906
sequence: https://synbiohub.org/public/igem/BBa_K1033905_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2349945
display id: annotation2349945
version: 1
top level: tsPurple
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349945/range2349945/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349945/range2349945/1
type: Range
persistent identity: range2349945
display id: range2349945
version: 1
direction: direction/forward
top level: tsPurple
start: 1
end: 12
orientation: inline

tsPurple
type: SequenceAnnotation
persistent identity: annotation2349943
display id: annotation2349943
version: 1
top level: tsPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349943/range2349943/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349943/range2349943/1
type: Range
persistent identity: range2349943
display id: range2349943
version: 1
direction: direction/forward
top level: tsPurple
start: 19
end: 705
orientation: inline

BBa_K1033906
type: SequenceAnnotation
persistent identity: annotation2349944
display id: annotation2349944
version: 1
top level: tsPurple
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033905/annotation2349944/range2349944/1

https://synbiohub.org/public/igem/BBa_K1033905/annotation2349944/range2349944/1
type: Range
persistent identity: range2349944
display id: range2349944
version: 1
direction: direction/reverse
top level: tsPurple
start: 19
end: 708
orientation: inline

BBa_K1685005
type: ComponentDefinition
persistent identity: BBa K1685005
display id: BBa_K1685005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue
creator: ITB_Indonesia 2015
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:09:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 4206
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a blue chromoprotein named aeBlue. This part is useful for reporter.
mutable notes: The part has been codon-optimised for expression in E. coli using http://sg.idtdna.com/CodonOpt
mutable provenance: The aeBlue sequence from Uppsala team 2012 (BBa_K864401) was chemically synthesised.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1685005_sequence/1

BBa_K1467201
type: ComponentDefinition
persistent identity: BBa K1467201
display id: BBa_K1467201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible AmilCP, Blue chromoprotein
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:04:22Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_K592009 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences. The chromoprotein naturally exhibits strong blue color when expressed. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domesticated for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467201_sequence/1

BBa_K1033933
type: ComponentDefinition
persistent identity: BBa K1033933
display id: BBa_K1033933
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: asPink
sequence: https://synbiohub.org/public/igem/BBa_K1033933_sequence/1

asPink
type: SequenceAnnotation
persistent identity: annotation2379047
display id: annotation2379047
version: 1
top level: BBa_K1033933
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1

https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1
type: Range
persistent identity: range2379047
display id: range2379047
version: 1
direction: direction/forward
top level: BBa_K1033933
start: 1
end: 702
orientation: inline

BBa_K1824017
type: ComponentDefinition
persistent identity: BBa K1824017
display id: BBa_K1824017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue blue chromoprotein
creator: Zhe Yang
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:07:38Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 4206
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable description: AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012.
mutable notes: No
mutable provenance: From Uppsala University in 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AeBlue chromoprotein
sequence: https://synbiohub.org/public/igem/BBa_K1824017_sequence/1

AeBlue chromoprotein
type: SequenceAnnotation
persistent identity: annotation2455427
display id: annotation2455427
version: 1
top level: BBa_K1824017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1824017/annotation2455427/range2455427/1

https://synbiohub.org/public/igem/BBa_K1824017/annotation2455427/range2455427/1
type: Range
persistent identity: range2455427
display id: range2455427
version: 1
direction: direction/forward
top level: BBa_K1824017
start: 1
end: 699
orientation: inline

BBa_K1680021
type: ComponentDefinition
persistent identity: BBa K1680021
display id: BBa_K1680021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T01:27:03Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cre recombinase
sequence annotation: linker
sequence annotation: SV40 NLS
sequence annotation: Dronpa
sequence: https://synbiohub.org/public/igem/BBa_K1680021_sequence/1

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477376
display id: annotation2477376
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1
type: Range
persistent identity: range2477376
display id: range2477376
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 718
end: 1746
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477375
display id: annotation2477375
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1
type: Range
persistent identity: range2477375
display id: range2477375
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 700
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477373
display id: annotation2477373
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1
type: Range
persistent identity: range2477373
display id: range2477373
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477374
display id: annotation2477374
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1
type: Range
persistent identity: range2477374
display id: range2477374
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 31
end: 699
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477377
display id: annotation2477377
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1
type: Range
persistent identity: range2477377
display id: range2477377
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1747
end: 1764
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477378
display id: annotation2477378
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1
type: Range
persistent identity: range2477378
display id: range2477378
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1765
end: 2433
orientation: inline

BBa_K1943007
type: ComponentDefinition
persistent identity: BBa K1943007
display id: BBa_K1943007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, Pink chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:40:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943007 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1033932
component: BBa_K608007
sequence annotation: BBa_B0015
sequence annotation: BBa_K608007
sequence annotation: BBa_K1033932
sequence: https://synbiohub.org/public/igem/BBa_K1943007_sequence/1

BBa_B0015
type: Component
persistent identity: component2481942
display id: component2481942
version: 1
top level: BBa_K1943007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033932
type: Component
persistent identity: component2481935
display id: component2481935
version: 1
top level: BBa_K1943007
access: public
definition: BBa_K1033932

BBa_K608007
type: Component
persistent identity: component2481933
display id: component2481933
version: 1
top level: BBa_K1943007
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481942
display id: annotation2481942
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481942/range2481942/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481942/range2481942/1
type: Range
persistent identity: range2481942
display id: range2481942
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 750
end: 878
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481933
display id: annotation2481933
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481933/range2481933/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481933/range2481933/1
type: Range
persistent identity: range2481933
display id: range2481933
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 1
end: 57
orientation: inline

BBa_K1033932
type: SequenceAnnotation
persistent identity: annotation2481935
display id: annotation2481935
version: 1
top level: BBa_K1943007
location: https://synbiohub.org/public/igem/BBa_K1943007/annotation2481935/range2481935/1
component: BBa_K1033932

https://synbiohub.org/public/igem/BBa_K1943007/annotation2481935/range2481935/1
type: Range
persistent identity: range2481935
display id: range2481935
version: 1
direction: direction/reverse
top level: BBa_K1943007
start: 64
end: 741
orientation: inline

BBa_K1033932
type: ComponentDefinition
persistent identity: BBa K1033932
display id: BBa_K1033932
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink (also known as spisCP), naturally exhibits strong color when expressed. The protein has an absorption maximum at 560 nm giving it a pink color visible to the naked eye. The strong color is readily observed in both LB or on agar plates after less than 24 hours of incubation. The protein spisPink has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata. The protein was first extracted and characterized by Alieva et. al. under the name spisCP (GenBank: ABB17971.1). This version is codon optimized for E coli by Genscript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spisPink
sequence: https://synbiohub.org/public/igem/BBa_K1033932_sequence/1

spisPink
type: SequenceAnnotation
persistent identity: annotation2372666
display id: annotation2372666
version: 1
top level: BBa_K1033932
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1

https://synbiohub.org/public/igem/BBa_K1033932/annotation2372666/range2372666/1
type: Range
persistent identity: range2372666
display id: range2372666
version: 1
direction: direction/forward
top level: BBa_K1033932
start: 1
end: 678
orientation: inline

BBa_K1023005
type: ComponentDefinition
persistent identity: BBa K1023005
display id: BBa_K1023005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed
creator: Michiel Karrenbelt
created: 2013-09-16T11:00:00Z
modified: 2016-01-26T03:04:25Z
discontinued: false
dominant: false
group u list: _1330_
m user id: 4206
owner id: 15730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: codon optimised chromoprotein
mutable notes: codon optimised
mutable provenance: Braunschweig via Uppsala
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1023005_sequence/1

BBa_K1484007
type: ComponentDefinition
persistent identity: BBa K1484007
display id: BBa_K1484007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cjBlue chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:17:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: cjBlue chromoprotein in BBa and MoClo standard
mutable notes: The cjBlue chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484007_sequence/1

BBa_K1943003
type: ComponentDefinition
persistent identity: BBa K1943003
display id: BBa_K1943003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:34:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943003 with chromoprotein gfasPurple(BBa_K1033919) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033919
component: BBa_B0015
component: BBa_K608007
sequence annotation: BBa_B0015
sequence annotation: BBa_K608007
sequence annotation: BBa_K1033919
sequence: https://synbiohub.org/public/igem/BBa_K1943003_sequence/1

BBa_K1033919
type: Component
persistent identity: component2481848
display id: component2481848
version: 1
top level: BBa_K1943003
access: public
definition: gfasPurple

BBa_B0015
type: Component
persistent identity: component2481855
display id: component2481855
version: 1
top level: BBa_K1943003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K608007
type: Component
persistent identity: component2481846
display id: component2481846
version: 1
top level: BBa_K1943003
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481855
display id: annotation2481855
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481855/range2481855/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481855/range2481855/1
type: Range
persistent identity: range2481855
display id: range2481855
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 741
end: 869
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481846
display id: annotation2481846
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481846/range2481846/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481846/range2481846/1
type: Range
persistent identity: range2481846
display id: range2481846
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 1
end: 57
orientation: inline

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2481848
display id: annotation2481848
version: 1
top level: BBa_K1943003
location: https://synbiohub.org/public/igem/BBa_K1943003/annotation2481848/range2481848/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1943003/annotation2481848/range2481848/1
type: Range
persistent identity: range2481848
display id: range2481848
version: 1
direction: direction/reverse
top level: BBa_K1943003
start: 64
end: 732
orientation: inline

BBa_K1399003
type: ComponentDefinition
persistent identity: BBa K1399003
display id: BBa_K1399003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added DAS-ssrA degradation tag (part BBa_M0052). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYADAS and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0052, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RFP
sequence annotation: start
sequence annotation: stop
sequence annotation: DAS-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399003_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2383904
display id: annotation2383904
version: 1
top level: BBa_K1399003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1
type: Range
persistent identity: range2383904
display id: range2383904
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 4
end: 675
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383903
display id: annotation2383903
version: 1
top level: BBa_K1399003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1
type: Range
persistent identity: range2383903
display id: range2383903
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383907
display id: annotation2383907
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1
type: Range
persistent identity: range2383907
display id: range2383907
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 712
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383906
display id: annotation2383906
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1
type: Range
persistent identity: range2383906
display id: range2383906
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 709
end: 711
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383905
display id: annotation2383905
version: 1
top level: BBa_K1399003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1
type: Range
persistent identity: range2383905
display id: range2383905
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 676
end: 708
orientation: inline

BBa_K1943001
type: ComponentDefinition
persistent identity: BBa K1943001
display id: BBa_K1943001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:02:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943001 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K608007
component: BBa_K1033910
component: BBa_B0015
sequence annotation: BBa_K608007
sequence annotation: BBa_B0015
sequence annotation: BBa_K1033910
sequence: https://synbiohub.org/public/igem/BBa_K1943001_sequence/1

BBa_K608007
type: Component
persistent identity: component2481871
display id: component2481871
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1033910
type: Component
persistent identity: component2481873
display id: component2481873
version: 1
top level: BBa_K1943001
access: public
definition: fwYellow

BBa_B0015
type: Component
persistent identity: component2481880
display id: component2481880
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481871
display id: annotation2481871
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
type: Range
persistent identity: range2481871
display id: range2481871
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 1
end: 57
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481880
display id: annotation2481880
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
type: Range
persistent identity: range2481880
display id: range2481880
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 786
end: 914
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2481873
display id: annotation2481873
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
type: Range
persistent identity: range2481873
display id: range2481873
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 64
end: 777
orientation: inline

eforRed
type: ComponentDefinition
persistent identity: BBa K592012
display id: BBa_K592012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, red chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein eforRed naturally exhibits red color when expressed. The color is weaker than RFP, however. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: coming soon
mutable provenance: coming soon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: eforRed
sequence: https://synbiohub.org/public/igem/BBa_K592012_sequence/1

eforRed
type: SequenceAnnotation
persistent identity: annotation2131799
display id: annotation2131799
version: 1
top level: eforRed
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1

https://synbiohub.org/public/igem/BBa_K592012/annotation2131799/range2131799/1
type: Range
persistent identity: range2131799
display id: range2131799
version: 1
direction: direction/forward
top level: eforRed
start: 1
end: 681
orientation: inline

BBa_K1399000
type: ComponentDefinition
persistent identity: BBa K1399000
display id: BBa_K1399000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part E1010) with added AAV-ssrA degradation tag (part I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: 1) ClpXP and ClpAP protease and SspB mediator concentrations; 2) Protein stability; 3) Km of binding to the protease; 4) Temperature [3]. [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3]Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part E1010, tag sequence was obtained from part I11012, but same tag was also usead in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cds
sequence annotation: AAV-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383858
display id: annotation2383858
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1
type: Range
persistent identity: range2383858
display id: range2383858
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 712
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383857
display id: annotation2383857
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1
type: Range
persistent identity: range2383857
display id: range2383857
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 709
end: 711
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383855
display id: annotation2383855
version: 1
top level: BBa_K1399000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1
type: Range
persistent identity: range2383855
display id: range2383855
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 4
end: 675
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383856
display id: annotation2383856
version: 1
top level: BBa_K1399000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1
type: Range
persistent identity: range2383856
display id: range2383856
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 676
end: 708
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383854
display id: annotation2383854
version: 1
top level: BBa_K1399000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1
type: Range
persistent identity: range2383854
display id: range2383854
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 1
end: 3
orientation: inline

BBa_K1484001
type: ComponentDefinition
persistent identity: BBa K1484001
display id: BBa_K1484001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple chromoprotein in GoldenGate standard
creator: Virginie Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:04:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tsPurple chromoprotein in BBa and MoClo standard
mutable notes: The tsPurple chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: goldengate fusion sequence
sequence annotation: BsaI recognition site
sequence annotation: tsPurple
sequence: https://synbiohub.org/public/igem/BBa_K1484001_sequence/1

goldengate fusion sequence
type: SequenceAnnotation
persistent identity: annotation2393992
display id: annotation2393992
version: 1
top level: BBa_K1484001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484001/annotation2393992/range2393992/1

https://synbiohub.org/public/igem/BBa_K1484001/annotation2393992/range2393992/1
type: Range
persistent identity: range2393992
display id: range2393992
version: 1
direction: direction/forward
top level: BBa_K1484001
start: 8
end: 11
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2393990
display id: annotation2393990
version: 1
top level: BBa_K1484001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484001/annotation2393990/range2393990/1

https://synbiohub.org/public/igem/BBa_K1484001/annotation2393990/range2393990/1
type: Range
persistent identity: range2393990
display id: range2393990
version: 1
direction: direction/forward
top level: BBa_K1484001
start: 1
end: 6
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2393991
display id: annotation2393991
version: 1
top level: BBa_K1484001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484001/annotation2393991/range2393991/1

https://synbiohub.org/public/igem/BBa_K1484001/annotation2393991/range2393991/1
type: Range
persistent identity: range2393991
display id: range2393991
version: 1
direction: direction/reverse
top level: BBa_K1484001
start: 704
end: 709
orientation: inline

goldengate fusion sequence
type: SequenceAnnotation
persistent identity: annotation2393993
display id: annotation2393993
version: 1
top level: BBa_K1484001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484001/annotation2393993/range2393993/1

https://synbiohub.org/public/igem/BBa_K1484001/annotation2393993/range2393993/1
type: Range
persistent identity: range2393993
display id: range2393993
version: 1
direction: direction/forward
top level: BBa_K1484001
start: 699
end: 702
orientation: inline

tsPurple
type: SequenceAnnotation
persistent identity: annotation2393994
display id: annotation2393994
version: 1
top level: BBa_K1484001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1484001/annotation2393994/range2393994/1

https://synbiohub.org/public/igem/BBa_K1484001/annotation2393994/range2393994/1
type: Range
persistent identity: range2393994
display id: range2393994
version: 1
direction: direction/forward
top level: BBa_K1484001
start: 9
end: 698
orientation: inline

spisPink
type: ComponentDefinition
persistent identity: BBa K1033925
display id: BBa_K1033925
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spisPink, pink chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Stylophora pistillata, spisPink, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Stylophora pistillata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: spisPink
sequence: https://synbiohub.org/public/igem/BBa_K1033925_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2351830
display id: annotation2351830
version: 1
top level: spisPink
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351830/range2351830/1
type: Range
persistent identity: range2351830
display id: range2351830
version: 1
direction: direction/forward
top level: spisPink
start: 5
end: 13
orientation: inline

spisPink
type: SequenceAnnotation
persistent identity: annotation2351831
display id: annotation2351831
version: 1
top level: spisPink
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1

https://synbiohub.org/public/igem/BBa_K1033925/annotation2351831/range2351831/1
type: Range
persistent identity: range2351831
display id: range2351831
version: 1
direction: direction/forward
top level: spisPink
start: 20
end: 694
orientation: inline

BBa_K1943002
type: ComponentDefinition
persistent identity: BBa K1943002
display id: BBa_K1943002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:03:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943002 with chromoprotein gfasPurple(BBa_K1033919) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K880005
component: BBa_K1033919
sequence annotation: BBa_K1033919
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943002_sequence/1

BBa_B0015
type: Component
persistent identity: component2512733
display id: component2512733
version: 1
top level: BBa_K1943002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: Component
persistent identity: component2512724
display id: component2512724
version: 1
top level: BBa_K1943002
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_K1033919
type: Component
persistent identity: component2512726
display id: component2512726
version: 1
top level: BBa_K1943002
access: public
definition: gfasPurple

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2512726
display id: annotation2512726
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512726/range2512726/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512726/range2512726/1
type: Range
persistent identity: range2512726
display id: range2512726
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 62
end: 730
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512724
display id: annotation2512724
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512724/range2512724/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512724/range2512724/1
type: Range
persistent identity: range2512724
display id: range2512724
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512733
display id: annotation2512733
version: 1
top level: BBa_K1943002
location: https://synbiohub.org/public/igem/BBa_K1943002/annotation2512733/range2512733/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943002/annotation2512733/range2512733/1
type: Range
persistent identity: range2512733
display id: range2512733
version: 1
direction: direction/reverse
top level: BBa_K1943002
start: 739
end: 867
orientation: inline

BBa_K1484006
type: ComponentDefinition
persistent identity: BBa K1484006
display id: BBa_K1484006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EforRed chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:17:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EforRed chromoprotein in BBa and MoClo standard
mutable notes: The EforRed chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484006_sequence/1

BBa_K1680020
type: ComponentDefinition
persistent identity: BBa K1680020
display id: BBa_K1680020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:16:19Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680006
sequence annotation: K1680003
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680020_sequence/1

K1680006
type: SequenceAnnotation
persistent identity: annotation2477364
display id: annotation2477364
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1
type: Range
persistent identity: range2477364
display id: range2477364
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1114
end: 1782
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477366
display id: annotation2477366
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1
type: Range
persistent identity: range2477366
display id: range2477366
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1089
end: 1113
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477363
display id: annotation2477363
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1
type: Range
persistent identity: range2477363
display id: range2477363
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 40
end: 1068
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477365
display id: annotation2477365
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1
type: Range
persistent identity: range2477365
display id: range2477365
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1
end: 39
orientation: inline

BBa_K1886005
type: ComponentDefinition
persistent identity: BBa K1886005
display id: BBa_K1886005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter-ho1-pcyA
creator: Lejian Jiang
created: 2016-10-05T11:00:00Z
modified: 2016-10-17T01:11:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This circuit is composed of the constitutive promoter, ho1 chromoprotein and pcya chormoprotein. They facilitate Membrane protein ccaS and cph8 to be activated by light of certain wavelength.
mutable notes: Protein ho1 and protein pcyA have to combine with each other to function, so we put them behind the same promoter.
mutable provenance: This part is from paper "Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems", and obtained from iGEM team HZAU's lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_J23108
component: BBa_I15009
component: BBa_B0015
component: BBa_I15008
sequence annotation: BBa_B0034
sequence annotation: BBa_J23108
sequence annotation: BBa_I15008
sequence annotation: BBa_B0015
sequence annotation: BBa_I15009
sequence: https://synbiohub.org/public/igem/BBa_K1886005_sequence/1

BBa_B0034
type: Component
persistent identity: component2486589
display id: component2486589
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23108
type: Component
persistent identity: component2486583
display id: component2486583
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23108/1

BBa_B0034
type: Component
persistent identity: component2486585
display id: component2486585
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15009
type: Component
persistent identity: component2486592
display id: component2486592
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0015
type: Component
persistent identity: component2486599
display id: component2486599
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I15008
type: Component
persistent identity: component2486587
display id: component2486587
version: 1
top level: BBa_K1886005
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2486585
display id: annotation2486585
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486585/range2486585/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486585/range2486585/1
type: Range
persistent identity: range2486585
display id: range2486585
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 44
end: 55
orientation: inline

BBa_J23108
type: SequenceAnnotation
persistent identity: annotation2486583
display id: annotation2486583
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486583/range2486583/1
component: BBa_J23108

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486583/range2486583/1
type: Range
persistent identity: range2486583
display id: range2486583
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 1
end: 35
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2486587
display id: annotation2486587
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486587/range2486587/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486587/range2486587/1
type: Range
persistent identity: range2486587
display id: range2486587
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 62
end: 812
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2486589
display id: annotation2486589
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486589/range2486589/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486589/range2486589/1
type: Range
persistent identity: range2486589
display id: range2486589
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 821
end: 832
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2486599
display id: annotation2486599
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486599/range2486599/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486599/range2486599/1
type: Range
persistent identity: range2486599
display id: range2486599
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 1622
end: 1750
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2486592
display id: annotation2486592
version: 1
top level: BBa_K1886005
location: https://synbiohub.org/public/igem/BBa_K1886005/annotation2486592/range2486592/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K1886005/annotation2486592/range2486592/1
type: Range
persistent identity: range2486592
display id: range2486592
version: 1
direction: direction/reverse
top level: BBa_K1886005
start: 839
end: 1613
orientation: inline

BBa_K1680017
type: ComponentDefinition
persistent identity: BBa K1680017
display id: BBa_K1680017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa Fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:17Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly.
mutable provenance: gBlocks designed by Team Tuebingen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1680000
sequence annotation: BBa_K1680007
sequence annotation: BBa_K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680017_sequence/1

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465978
display id: annotation2465978
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1
type: Range
persistent identity: range2465978
display id: range2465978
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1050
end: 1055
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465976
display id: annotation2465976
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1
type: Range
persistent identity: range2465976
display id: range2465976
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1
end: 6
orientation: inline

BBa_K1680007
type: SequenceAnnotation
persistent identity: annotation2465977
display id: annotation2465977
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1
type: Range
persistent identity: range2465977
display id: range2465977
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 13
end: 1041
orientation: inline

BBa_K1680006
type: SequenceAnnotation
persistent identity: annotation2465979
display id: annotation2465979
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1
type: Range
persistent identity: range2465979
display id: range2465979
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1064
end: 1732
orientation: inline

BBa_K1943000
type: ComponentDefinition
persistent identity: BBa K1943000
display id: BBa_K1943000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:00:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943000 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: 11
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K880005
component: BBa_B0015
component: BBa_K1033910
sequence annotation: BBa_K1033910
sequence annotation: BBa_B0015
sequence annotation: BBa_K880005
sequence: https://synbiohub.org/public/igem/BBa_K1943000_sequence/1

BBa_K880005
type: Component
persistent identity: component2512711
display id: component2512711
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_B0015
type: Component
persistent identity: component2512720
display id: component2512720
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033910
type: Component
persistent identity: component2512713
display id: component2512713
version: 1
top level: BBa_K1943000
access: public
definition: fwYellow

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2512713
display id: annotation2512713
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512713/range2512713/1
component: BBa_K1033910

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type: Range
persistent identity: range2512713
display id: range2512713
version: 1
direction: direction/reverse
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start: 62
end: 775
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512720
display id: annotation2512720
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512720/range2512720/1
component: BBa_B0015

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type: Range
persistent identity: range2512720
display id: range2512720
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 784
end: 912
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512711
display id: annotation2512711
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512711/range2512711/1
component: BBa_K880005

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type: Range
persistent identity: range2512711
display id: range2512711
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 1
end: 55
orientation: inline

BBa_K1399001
type: ComponentDefinition
persistent identity: BBa K1399001
display id: BBa_K1399001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. This tag is commonly attached to repressor proteins for use in various gene networks (e.g., oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; protein stability; Km of binding to the protease; temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction sites. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: cds
sequence annotation: LVA-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383872
display id: annotation2383872
version: 1
top level: BBa_K1399001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1

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type: Range
persistent identity: range2383872
display id: range2383872
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383875
display id: annotation2383875
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1

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type: Range
persistent identity: range2383875
display id: range2383875
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 709
end: 711
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383876
display id: annotation2383876
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1

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type: Range
persistent identity: range2383876
display id: range2383876
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 712
end: 714
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383873
display id: annotation2383873
version: 1
top level: BBa_K1399001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1

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type: Range
persistent identity: range2383873
display id: range2383873
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 4
end: 675
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383874
display id: annotation2383874
version: 1
top level: BBa_K1399001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1

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type: Range
persistent identity: range2383874
display id: range2383874
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 676
end: 708
orientation: inline

BBa_K1680019
type: ComponentDefinition
persistent identity: BBa K1680019
display id: BBa_K1680019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:55Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680019_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477415
display id: annotation2477415
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1

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type: Range
persistent identity: range2477415
display id: range2477415
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1
end: 30
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477418
display id: annotation2477418
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1

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type: Range
persistent identity: range2477418
display id: range2477418
version: 1
direction: direction/forward
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start: 1096
end: 1764
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477417
display id: annotation2477417
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1

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type: Range
persistent identity: range2477417
display id: range2477417
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1060
end: 1095
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477416
display id: annotation2477416
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1
type: Range
persistent identity: range2477416
display id: range2477416
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 31
end: 1059
orientation: inline

BBa_K1943004
type: ComponentDefinition
persistent identity: BBa K1943004
display id: BBa_K1943004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, Red chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:38:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943004 with chromoprotein eforRed(BBa_K592012) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K592012
component: BBa_K880005
sequence annotation: BBa_B0015
sequence annotation: BBa_K592012
sequence annotation: BBa_K880005
sequence: https://synbiohub.org/public/igem/BBa_K1943004_sequence/1

BBa_B0015
type: Component
persistent identity: component2512746
display id: component2512746
version: 1
top level: BBa_K1943004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K592012
type: Component
persistent identity: component2512739
display id: component2512739
version: 1
top level: BBa_K1943004
access: public
definition: eforRed

BBa_K880005
type: Component
persistent identity: component2512737
display id: component2512737
version: 1
top level: BBa_K1943004
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512746
display id: annotation2512746
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512746/range2512746/1
component: BBa_B0015

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type: Range
persistent identity: range2512746
display id: range2512746
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 751
end: 879
orientation: inline

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2512739
display id: annotation2512739
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512739/range2512739/1
component: BBa_K592012

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type: Range
persistent identity: range2512739
display id: range2512739
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 62
end: 742
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512737
display id: annotation2512737
version: 1
top level: BBa_K1943004
location: https://synbiohub.org/public/igem/BBa_K1943004/annotation2512737/range2512737/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943004/annotation2512737/range2512737/1
type: Range
persistent identity: range2512737
display id: range2512737
version: 1
direction: direction/reverse
top level: BBa_K1943004
start: 1
end: 55
orientation: inline

BBa_K1886004
type: ComponentDefinition
persistent identity: BBa K1886004
display id: BBa_K1886004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sll1472 ccaS
creator: Lejian Jiang
created: 2016-10-05T11:00:00Z
modified: 2016-10-17T12:57:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This circuit is composed of the constitutive promoter and photosensitive protein gene ccaS . ccaS protein is membrane protein. After being activated by 520-nm light, it could transfer its phosphoryl group to the intracellular protein ccaR,and this process could be inhibited by 650-nm light
mutable notes: It's not difficult to construct this part,but the strength of the promoter and the RBS will influence the abundance of protein ccaS,and finally influence the whole TCS.And there was a SpeI site and a EcoRI site,so we designed two silent mutations.The upstream of gene ccaS called sll1472 is of unknown function.
mutable provenance: Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ccaS
sequence annotation: unknown area
sequence: https://synbiohub.org/public/igem/BBa_K1886004_sequence/1

ccaS
type: SequenceAnnotation
persistent identity: annotation2514809
display id: annotation2514809
version: 1
top level: BBa_K1886004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1886004/annotation2514809/range2514809/1

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type: Range
persistent identity: range2514809
display id: range2514809
version: 1
direction: direction/forward
top level: BBa_K1886004
start: 472
end: 2733
orientation: inline

unknown area
type: SequenceAnnotation
persistent identity: annotation2514810
display id: annotation2514810
version: 1
top level: BBa_K1886004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1886004/annotation2514810/range2514810/1

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type: Range
persistent identity: range2514810
display id: range2514810
version: 1
direction: direction/forward
top level: BBa_K1886004
start: 1
end: 471
orientation: inline

BBa_K1399002
type: ComponentDefinition
persistent identity: BBa K1399002
display id: BBa_K1399002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; Protein stability; Km of binding to the protease; Temperature [3]. Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease a…
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0050, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: RFP
sequence annotation: tag
sequence: https://synbiohub.org/public/igem/BBa_K1399002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383898
display id: annotation2383898
version: 1
top level: BBa_K1399002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383898/range2383898/1

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type: Range
persistent identity: range2383898
display id: range2383898
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383901
display id: annotation2383901
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383901/range2383901/1

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type: Range
persistent identity: range2383901
display id: range2383901
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 709
end: 711
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383902
display id: annotation2383902
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1

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type: Range
persistent identity: range2383902
display id: range2383902
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 712
end: 714
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383899
display id: annotation2383899
version: 1
top level: BBa_K1399002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383899/range2383899/1

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type: Range
persistent identity: range2383899
display id: range2383899
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 4
end: 675
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383900
display id: annotation2383900
version: 1
top level: BBa_K1399002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1
type: Range
persistent identity: range2383900
display id: range2383900
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 676
end: 708
orientation: inline

BBa_K1362461
type: ComponentDefinition
persistent identity: BBa K1362461
display id: BBa_K1362461
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2016-01-25T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 4206
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is essentially identical to <partinfo>BBa_E1010</partinfo>, but has a point mutation in the barcode. Barcodes are discontinued, but composite parts using this part will have the mutated barcode.
mutable provenance: ''Discosoma striata''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mutated barcode
sequence annotation: mrfp1
sequence: https://synbiohub.org/public/igem/BBa_K1362461_sequence/1

mutated barcode
type: SequenceAnnotation
persistent identity: annotation2404604
display id: annotation2404604
version: 1
top level: BBa_K1362461
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1
type: Range
persistent identity: range2404604
display id: range2404604
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 682
end: 706
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2404599
display id: annotation2404599
version: 1
top level: BBa_K1362461
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1
type: Range
persistent identity: range2404599
display id: range2404599
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 1
end: 675
orientation: inline

BBa_K1680023
type: ComponentDefinition
persistent identity: BBa K1680023
display id: BBa_K1680023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:42:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680004
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680023_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477384
display id: annotation2477384
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1
type: Range
persistent identity: range2477384
display id: range2477384
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1765
end: 1800
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477379
display id: annotation2477379
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1
type: Range
persistent identity: range2477379
display id: range2477379
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1
end: 24
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477382
display id: annotation2477382
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1
type: Range
persistent identity: range2477382
display id: range2477382
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 736
end: 1764
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477383
display id: annotation2477383
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1
type: Range
persistent identity: range2477383
display id: range2477383
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 700
end: 735
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477381
display id: annotation2477381
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1
type: Range
persistent identity: range2477381
display id: range2477381
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1801
end: 2469
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477380
display id: annotation2477380
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1
type: Range
persistent identity: range2477380
display id: range2477380
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 31
end: 699
orientation: inline

BBa_K1122007
type: ComponentDefinition
persistent identity: BBa K1122007
display id: BBa_K1122007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmilCP (BBa_K592009) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:13:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: AmilCP (BBa_K592009) part with inward facing EarI recognition sites at the ends. It is compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_K592009.
mutable provenance: AmilCP is based on the BioBrick BBa_K592009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EarI site
sequence annotation: AmilCP
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1122007_sequence/1

EarI site
type: SequenceAnnotation
persistent identity: annotation2346347
display id: annotation2346347
version: 1
top level: BBa_K1122007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346347/range2346347/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346347/range2346347/1
type: Range
persistent identity: range2346347
display id: range2346347
version: 1
direction: direction/reverse
top level: BBa_K1122007
start: 680
end: 685
orientation: inline

AmilCP
type: SequenceAnnotation
persistent identity: annotation2346346
display id: annotation2346346
version: 1
top level: BBa_K1122007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346346/range2346346/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346346/range2346346/1
type: Range
persistent identity: range2346346
display id: range2346346
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 1
end: 681
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2346344
display id: annotation2346344
version: 1
top level: BBa_K1122007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346344/range2346344/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346344/range2346344/1
type: Range
persistent identity: range2346344
display id: range2346344
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 6
end: 11
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2346345
display id: annotation2346345
version: 1
top level: BBa_K1122007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346345/range2346345/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346345/range2346345/1
type: Range
persistent identity: range2346345
display id: range2346345
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 679
end: 681
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2346343
display id: annotation2346343
version: 1
top level: BBa_K1122007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122007/annotation2346343/range2346343/1

https://synbiohub.org/public/igem/BBa_K1122007/annotation2346343/range2346343/1
type: Range
persistent identity: range2346343
display id: range2346343
version: 1
direction: direction/forward
top level: BBa_K1122007
start: 1
end: 3
orientation: inline

gfasPurple
type: ComponentDefinition
persistent identity: BBa K1033919
display id: BBa_K1033919
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Galaxea fascicularis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gfasPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033919_sequence/1

gfasPurple
type: SequenceAnnotation
persistent identity: annotation2332104
display id: annotation2332104
version: 1
top level: gfasPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1

https://synbiohub.org/public/igem/BBa_K1033919/annotation2332104/range2332104/1
type: Range
persistent identity: range2332104
display id: range2332104
version: 1
direction: direction/forward
top level: gfasPurple
start: 1
end: 666
orientation: inline

BBa_K2078002
type: ComponentDefinition
persistent identity: BBa K2078002
display id: BBa_K2078002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119 + amilCP
creator: Adam Zivanic
created: 2016-10-05T11:00:00Z
modified: 2016-10-09T02:25:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2546_
m user id: 30604
owner id: 30629
owning group id: 9
status: status/Planning
bookmark: false
mutable description: J23119 + amilCP
mutable notes: None
mutable provenance: J23119 + K592025
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592025
component: BBa_J23119
sequence annotation: BBa_K592025
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K2078002_sequence/1

BBa_K592025
type: Component
persistent identity: component2486726
display id: component2486726
version: 1
top level: BBa_K2078002
access: public
definition: https://synbiohub.org/public/igem/BBa_K592025/1

BBa_J23119
type: Component
persistent identity: component2486721
display id: component2486721
version: 1
top level: BBa_K2078002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_K592025
type: SequenceAnnotation
persistent identity: annotation2486726
display id: annotation2486726
version: 1
top level: BBa_K2078002
location: https://synbiohub.org/public/igem/BBa_K2078002/annotation2486726/range2486726/1
component: BBa_K592025

https://synbiohub.org/public/igem/BBa_K2078002/annotation2486726/range2486726/1
type: Range
persistent identity: range2486726
display id: range2486726
version: 1
direction: direction/reverse
top level: BBa_K2078002
start: 44
end: 730
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2486721
display id: annotation2486721
version: 1
top level: BBa_K2078002
location: https://synbiohub.org/public/igem/BBa_K2078002/annotation2486721/range2486721/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K2078002/annotation2486721/range2486721/1
type: Range
persistent identity: range2486721
display id: range2486721
version: 1
direction: direction/reverse
top level: BBa_K2078002
start: 1
end: 35
orientation: inline

cjBlue
type: ComponentDefinition
persistent identity: BBa K592011
display id: BBa_K592011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cjBlue, green chromoprotein
creator: Antonio Ascue Avalos
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein, cjBlue, naturally exhibits strong green/blue color when expressed. Compared to amilCP(BBa_K592009) and amilGFP(BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: Codon-optimization for expression in E.coli necessary.
mutable provenance: Cnidopus japonicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cjBlue
sequence: https://synbiohub.org/public/igem/BBa_K592011_sequence/1

cjBlue
type: SequenceAnnotation
persistent identity: annotation2131787
display id: annotation2131787
version: 1
top level: cjBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1

https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1
type: Range
persistent identity: range2131787
display id: range2131787
version: 1
direction: direction/forward
top level: cjBlue
start: 1
end: 699
orientation: inline

BBa_K1033916
type: ComponentDefinition
persistent identity: BBa K1033916
display id: BBa_K1033916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The anemone Anemonia majano.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amajLime
sequence: https://synbiohub.org/public/igem/BBa_K1033916_sequence/1

amajLime
type: SequenceAnnotation
persistent identity: annotation2332103
display id: annotation2332103
version: 1
top level: BBa_K1033916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1

https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
type: Range
persistent identity: range2332103
display id: range2332103
version: 1
direction: direction/forward
top level: BBa_K1033916
start: 1
end: 690
orientation: inline

BBa_K1744001
type: ComponentDefinition
persistent identity: BBa K1744001
display id: BBa_K1744001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P1U8-amilCP-kanR
creator: Kevin Neil
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T12:41:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2166_
m user id: 26754
owner id: 26754
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a positive selection system for recombineering. It contains a kanamycin resistance gene Aph(3???)-I (aminoglycoside 3???-phosphotransferase, truncated version) with its native promoter as well as amilCP, a deep blue chromoprotein. amilCP gene is under the control of a strong constitutive promoter with a strong RBS to make it more intense than previous versions. The cassette is designed to be a double positive selection marker for recombineering. Once amplified with primer bearing homology to the targeted region, the cassette can be integrated in the genome through lambda red recombination. Once integrated, the colonies should become resistant to kanamycin and become pale blue overtime. However, the blue color is actually faint and really slow to become apparent.
mutable notes: The amilCP chromoprotein synthesis could be improved for the blue color to be visible in single copy faster. It has been of great concern through-out all the project and that is why we have put the strongest combination of promoter and RBS we have found to be well characterised. Still, expression in single copy is not so intense. In our case, amilCP can still be useful as a marker for plasmid background detection during recombineering experiment. Using only the kanamycin marker for recombineering, the blue colonies would represent plasmid transformants and not good recombinants. This use is good for us though, since background is a great concern in recombineering techniques, where screening can be long.
mutable provenance: The part was constructed using a kanamycin resistance gene from plasmid pOK12 (available commercially) and amilCP from BBa_K592009. The promoter and RBS P1U8 were synthesised and come from Mutalik et al. (Nature 2013).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: amilCP
sequence annotation: Promoter P1
sequence annotation: U8
sequence annotation: aph-3'-I
sequence annotation: Promoter-aph-3'-I
sequence: https://synbiohub.org/public/igem/BBa_K1744001_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2454758
display id: annotation2454758
version: 1
top level: BBa_K1744001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1744001/annotation2454758/range2454758/1

https://synbiohub.org/public/igem/BBa_K1744001/annotation2454758/range2454758/1
type: Range
persistent identity: range2454758
display id: range2454758
version: 1
direction: direction/reverse
top level: BBa_K1744001
start: 820
end: 1488
orientation: inline

Promoter P1
type: SequenceAnnotation
persistent identity: annotation2454760
display id: annotation2454760
version: 1
top level: BBa_K1744001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1744001/annotation2454760/range2454760/1

https://synbiohub.org/public/igem/BBa_K1744001/annotation2454760/range2454760/1
type: Range
persistent identity: range2454760
display id: range2454760
version: 1
direction: direction/reverse
top level: BBa_K1744001
start: 1510
end: 1548
orientation: inline

U8
type: SequenceAnnotation
persistent identity: annotation2454759
display id: annotation2454759
version: 1
top level: BBa_K1744001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1744001/annotation2454759/range2454759/1

https://synbiohub.org/public/igem/BBa_K1744001/annotation2454759/range2454759/1
type: Range
persistent identity: range2454759
display id: range2454759
version: 1
direction: direction/reverse
top level: BBa_K1744001
start: 1489
end: 1509
orientation: inline

aph-3'-I
type: SequenceAnnotation
persistent identity: annotation2454745
display id: annotation2454745
version: 1
top level: BBa_K1744001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1744001/annotation2454745/range2454745/1

https://synbiohub.org/public/igem/BBa_K1744001/annotation2454745/range2454745/1
type: Range
persistent identity: range2454745
display id: range2454745
version: 1
direction: direction/reverse
top level: BBa_K1744001
start: 1
end: 612
orientation: inline

Promoter-aph-3'-I
type: SequenceAnnotation
persistent identity: annotation2454746
display id: annotation2454746
version: 1
top level: BBa_K1744001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1744001/annotation2454746/range2454746/1

https://synbiohub.org/public/igem/BBa_K1744001/annotation2454746/range2454746/1
type: Range
persistent identity: range2454746
display id: range2454746
version: 1
direction: direction/reverse
top level: BBa_K1744001
start: 613
end: 819
orientation: inline

BBa_K1484003
type: ComponentDefinition
persistent identity: BBa K1484003
display id: BBa_K1484003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue chromoprotein in BBa and MoClo standard
creator: ----
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:05:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AeBlue chromoprotein in BBa and MoClo standard
mutable notes: The AeBlue chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484003_sequence/1

BBa_K1484008
type: ComponentDefinition
persistent identity: BBa K1484008
display id: BBa_K1484008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue0034 chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:13:23Z
discontinued: false
dominant: false
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AeBlue0034 chromoprotein in BBa and MoClo standard
mutable notes: The tsPurple chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484008_sequence/1

BBa_K1680024
type: ComponentDefinition
persistent identity: BBa K1680024
display id: BBa_K1680024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:22:01Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly with RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680006
sequence annotation: K1680003
sequence annotation: K1680007
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680024_sequence/1

K1680006
type: SequenceAnnotation
persistent identity: annotation2477394
display id: annotation2477394
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1
type: Range
persistent identity: range2477394
display id: range2477394
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1818
end: 2487
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477393
display id: annotation2477393
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1
type: Range
persistent identity: range2477393
display id: range2477393
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 31
end: 699
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477396
display id: annotation2477396
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1
type: Range
persistent identity: range2477396
display id: range2477396
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1774
end: 1817
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477395
display id: annotation2477395
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1
type: Range
persistent identity: range2477395
display id: range2477395
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 700
end: 744
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477392
display id: annotation2477392
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1
type: Range
persistent identity: range2477392
display id: range2477392
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 745
end: 1773
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477390
display id: annotation2477390
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1
type: Range
persistent identity: range2477390
display id: range2477390
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1
end: 24
orientation: inline

BBa_K1680018
type: ComponentDefinition
persistent identity: BBa K1680018
display id: BBa_K1680018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:36Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly and RFC25.
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680001
sequence annotation: K1680006
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680018_sequence/1

K1680001
type: SequenceAnnotation
persistent identity: annotation2477357
display id: annotation2477357
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1
type: Range
persistent identity: range2477357
display id: range2477357
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1
end: 21
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477358
display id: annotation2477358
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1
type: Range
persistent identity: range2477358
display id: range2477358
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1051
end: 1077
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477354
display id: annotation2477354
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1
type: Range
persistent identity: range2477354
display id: range2477354
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1078
end: 1746
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477355
display id: annotation2477355
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1
type: Range
persistent identity: range2477355
display id: range2477355
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 22
end: 1050
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_K1484000
type: ComponentDefinition
persistent identity: BBa K1484000
display id: BBa_K1484000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AsPink chromoprotein in GoldenGate standard
creator: Virginie Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:03:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AsPink chromoprotein in BBa and MoClo standard.
mutable notes: The AsPink is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: asPink
sequence annotation: goldengate fusion sequence
sequence annotation: BsaI recognition site
sequence: https://synbiohub.org/public/igem/BBa_K1484000_sequence/1

asPink
type: SequenceAnnotation
persistent identity: annotation2393762
display id: annotation2393762
version: 1
top level: BBa_K1484000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1484000/annotation2393762/range2393762/1

https://synbiohub.org/public/igem/BBa_K1484000/annotation2393762/range2393762/1
type: Range
persistent identity: range2393762
display id: range2393762
version: 1
direction: direction/forward
top level: BBa_K1484000
start: 7
end: 710
orientation: inline

goldengate fusion sequence
type: SequenceAnnotation
persistent identity: annotation2393761
display id: annotation2393761
version: 1
top level: BBa_K1484000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484000/annotation2393761/range2393761/1

https://synbiohub.org/public/igem/BBa_K1484000/annotation2393761/range2393761/1
type: Range
persistent identity: range2393761
display id: range2393761
version: 1
direction: direction/reverse
top level: BBa_K1484000
start: 711
end: 714
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2393760
display id: annotation2393760
version: 1
top level: BBa_K1484000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484000/annotation2393760/range2393760/1

https://synbiohub.org/public/igem/BBa_K1484000/annotation2393760/range2393760/1
type: Range
persistent identity: range2393760
display id: range2393760
version: 1
direction: direction/reverse
top level: BBa_K1484000
start: 716
end: 721
orientation: inline

goldengate fusion sequence
type: SequenceAnnotation
persistent identity: annotation2393759
display id: annotation2393759
version: 1
top level: BBa_K1484000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484000/annotation2393759/range2393759/1

https://synbiohub.org/public/igem/BBa_K1484000/annotation2393759/range2393759/1
type: Range
persistent identity: range2393759
display id: range2393759
version: 1
direction: direction/forward
top level: BBa_K1484000
start: 8
end: 12
orientation: inline

BsaI recognition site
type: SequenceAnnotation
persistent identity: annotation2393758
display id: annotation2393758
version: 1
top level: BBa_K1484000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484000/annotation2393758/range2393758/1

https://synbiohub.org/public/igem/BBa_K1484000/annotation2393758/range2393758/1
type: Range
persistent identity: range2393758
display id: range2393758
version: 1
direction: direction/forward
top level: BBa_K1484000
start: 1
end: 6
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033910
display id: BBa_K1033910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2016-01-25T02:41:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 4206
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fwYellow
sequence: https://synbiohub.org/public/igem/BBa_K1033910_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2371218
display id: annotation2371218
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1

https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
type: Range
persistent identity: range2371218
display id: range2371218
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 711
orientation: inline

BBa_K1484005
type: ComponentDefinition
persistent identity: BBa K1484005
display id: BBa_K1484005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmilCP chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:12:56Z
discontinued: false
dominant: false
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AmilCP chromoprotein in BBa and MoClo standard
mutable notes: The AmilCP chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484005_sequence/1

BBa_K1680022
type: ComponentDefinition
persistent identity: BBa K1680022
display id: BBa_K1680022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:52:38Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 9 AA linker as spacer (BBa_K1680001). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cre recombinase
sequence annotation: Linker
sequence annotation: Dronpa
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1680022_sequence/1

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477388
display id: annotation2477388
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1
type: Range
persistent identity: range2477388
display id: range2477388
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 727
end: 1755
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477387
display id: annotation2477387
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1
type: Range
persistent identity: range2477387
display id: range2477387
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 700
end: 726
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477386
display id: annotation2477386
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1
type: Range
persistent identity: range2477386
display id: range2477386
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 31
end: 699
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477389
display id: annotation2477389
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1
type: Range
persistent identity: range2477389
display id: range2477389
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1756
end: 1783
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477391
display id: annotation2477391
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1
type: Range
persistent identity: range2477391
display id: range2477391
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1783
end: 2451
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477385
display id: annotation2477385
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1
type: Range
persistent identity: range2477385
display id: range2477385
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 4
end: 24
orientation: inline

meffBlue
type: ComponentDefinition
persistent identity: BBa K1033902
display id: BBa_K1033902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein
creator: Erik Gullberg
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Montipora efflorescens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: meffBlue
sequence: https://synbiohub.org/public/igem/BBa_K1033902_sequence/1

meffBlue
type: SequenceAnnotation
persistent identity: annotation2332099
display id: annotation2332099
version: 1
top level: meffBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1

https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1
type: Range
persistent identity: range2332099
display id: range2332099
version: 1
direction: direction/forward
top level: meffBlue
start: 1
end: 669
orientation: inline

amilGFP
type: ComponentDefinition
persistent identity: BBa K592010
display id: BBa_K592010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein, amilGFP, naturally exhibits very strong yellow color when expressed. The color is strong and readily visible to naked eye both in LB-culture and on agar plates. The DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) was removed.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amilGFP
sequence: https://synbiohub.org/public/igem/BBa_K592010_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2131633
display id: annotation2131633
version: 1
top level: amilGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1

https://synbiohub.org/public/igem/BBa_K592010/annotation2131633/range2131633/1
type: Range
persistent identity: range2131633
display id: range2131633
version: 1
direction: direction/forward
top level: amilGFP
start: 1
end: 696
orientation: inline

BBa_K1824019
type: ComponentDefinition
persistent identity: BBa K1824019
display id: BBa_K1824019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AeBlue blue chromoprotein with aav tag
creator: Zhe Yang
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:08:09Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 4206
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable description: AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.
mutable notes: No
mutable provenance: Chromoprotein is extracted from Uppsala University in 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aeblue chromoprotein
sequence annotation: aav tag
sequence: https://synbiohub.org/public/igem/BBa_K1824019_sequence/1

Aeblue chromoprotein
type: SequenceAnnotation
persistent identity: annotation2455430
display id: annotation2455430
version: 1
top level: BBa_K1824019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1824019/annotation2455430/range2455430/1

https://synbiohub.org/public/igem/BBa_K1824019/annotation2455430/range2455430/1
type: Range
persistent identity: range2455430
display id: range2455430
version: 1
direction: direction/forward
top level: BBa_K1824019
start: 1
end: 693
orientation: inline

aav tag
type: SequenceAnnotation
persistent identity: annotation2455431
display id: annotation2455431
version: 1
top level: BBa_K1824019
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1824019/annotation2455431/range2455431/1

https://synbiohub.org/public/igem/BBa_K1824019/annotation2455431/range2455431/1
type: Range
persistent identity: range2455431
display id: range2455431
version: 1
direction: direction/forward
top level: BBa_K1824019
start: 700
end: 735
orientation: inline

tsPurple
type: ComponentDefinition
persistent identity: BBa K1033906
display id: BBa_K1033906
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tsPurple
sequence: https://synbiohub.org/public/igem/BBa_K1033906_sequence/1

tsPurple
type: SequenceAnnotation
persistent identity: annotation2332100
display id: annotation2332100
version: 1
top level: tsPurple
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1

https://synbiohub.org/public/igem/BBa_K1033906/annotation2332100/range2332100/1
type: Range
persistent identity: range2332100
display id: range2332100
version: 1
direction: direction/forward
top level: tsPurple
start: 1
end: 690
orientation: inline

BBa_K1680006
type: ComponentDefinition
persistent identity: BBa K1680006
display id: BBa_K1680006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluorescent protein dronpa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 4206
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa, codon optimized for Yeast. The mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence: https://synbiohub.org/public/igem/BBa_K1680006_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2475196
display id: annotation2475196
version: 1
top level: BBa_K1680006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1

https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1
type: Range
persistent identity: range2475196
display id: range2475196
version: 1
direction: direction/forward
top level: BBa_K1680006
start: 1
end: 669
orientation: inline

BBa_K1484002
type: ComponentDefinition
persistent identity: BBa K1484002
display id: BBa_K1484002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmajLime chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:04:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AmajLime chromoprotein in BBa and MoClo standard
mutable notes: The AmajLime chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Bsai R
sequence annotation: BsaI F
sequence: https://synbiohub.org/public/igem/BBa_K1484002_sequence/1

Bsai R
type: SequenceAnnotation
persistent identity: annotation2419926
display id: annotation2419926
version: 1
top level: BBa_K1484002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484002/annotation2419926/range2419926/1

https://synbiohub.org/public/igem/BBa_K1484002/annotation2419926/range2419926/1
type: Range
persistent identity: range2419926
display id: range2419926
version: 1
direction: direction/forward
top level: BBa_K1484002
start: 706
end: 711
orientation: inline

BsaI F
type: SequenceAnnotation
persistent identity: annotation2419925
display id: annotation2419925
version: 1
top level: BBa_K1484002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1484002/annotation2419925/range2419925/1

https://synbiohub.org/public/igem/BBa_K1484002/annotation2419925/range2419925/1
type: Range
persistent identity: range2419925
display id: range2419925
version: 1
direction: direction/forward
top level: BBa_K1484002
start: 1
end: 6
orientation: inline

aeBlue
type: ComponentDefinition
persistent identity: BBa K864401
display id: BBa_K864401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue blue chromoprotein
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: aeBlue
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aeBlue
sequence: https://synbiohub.org/public/igem/BBa_K864401_sequence/1

aeBlue
type: SequenceAnnotation
persistent identity: annotation2197206
display id: annotation2197206
version: 1
top level: aeBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1

https://synbiohub.org/public/igem/BBa_K864401/annotation2197206/range2197206/1
type: Range
persistent identity: range2197206
display id: range2197206
version: 1
direction: direction/forward
top level: aeBlue
start: 1
end: 699
orientation: inline

BBa_K1943005
type: ComponentDefinition
persistent identity: BBa K1943005
display id: BBa_K1943005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eforRed, Red chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:38:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943005 with chromoprotein eforRed(BBa_K592012) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592012
component: BBa_B0015
component: BBa_K608007
sequence annotation: BBa_K592012
sequence annotation: BBa_K608007
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1943005_sequence/1

BBa_K592012
type: Component
persistent identity: component2481910
display id: component2481910
version: 1
top level: BBa_K1943005
access: public
definition: eforRed

BBa_B0015
type: Component
persistent identity: component2481917
display id: component2481917
version: 1
top level: BBa_K1943005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K608007
type: Component
persistent identity: component2481908
display id: component2481908
version: 1
top level: BBa_K1943005
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2481910
display id: annotation2481910
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481910/range2481910/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481910/range2481910/1
type: Range
persistent identity: range2481910
display id: range2481910
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 64
end: 744
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481908
display id: annotation2481908
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481908/range2481908/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481908/range2481908/1
type: Range
persistent identity: range2481908
display id: range2481908
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 1
end: 57
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481917
display id: annotation2481917
version: 1
top level: BBa_K1943005
location: https://synbiohub.org/public/igem/BBa_K1943005/annotation2481917/range2481917/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943005/annotation2481917/range2481917/1
type: Range
persistent identity: range2481917
display id: range2481917
version: 1
direction: direction/reverse
top level: BBa_K1943005
start: 753
end: 881
orientation: inline

BBa_K1484004
type: ComponentDefinition
persistent identity: BBa K1484004
display id: BBa_K1484004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmilGFP chromoprotein in BBa and MoClo standard
creator: Virginia Rutten
created: 2014-09-01T11:00:00Z
modified: 2015-06-10T02:13:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 22948
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AmilGFP chromoprotein in BBa and MoClo standard
mutable notes: The AmilGFP chromoprotein is now compatible with both standards and can be used more effectively.
mutable provenance: The chromoprotein was taken from the iGEM registry and altered to conform to the MoClo standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1484004_sequence/1