//cds/receptor
type: Collection
persistent identity: cds receptor
display id: cds_receptor
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K133062
member: BBa_K1909007
member: BBa_K133070
member: mTaz
member: virA
member: BBa_K1909010
member: BBa_K216003
member: CBPa-BpA
member: BBa_K103003
member: BBa_K1909003
member: BBa_K1405004
member: BBa_K1909006
member: BBa_K1909011
member: BBa_K216002
member: BBa_K1072010
member: BBa_K1909009
member: EnvZ Tail
member: BBa_K909009
member: BBa_K211001
member: BBa_K1909012
member: BBa_K515102
member: BBa_K1909008
member: BBa_I721002
member: BBa_K1909004
member: BBa_K211002
member: //cds/receptor/antibody
member: BBa_K2056000

//cds/receptor/antibody
type: Collection
persistent identity: cds receptor antibody
display id: cds_receptor_antibody
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K772002
member: DigA
member: BBa_K772001
member: BBa_K772100
member: BBa_K126000

mTaz
type: ComponentDefinition
persistent identity: BBa K1909002
display id: BBa_K1909002
version: 1
was generated by: iGEM to SBOL conversion
description: mTaz (Taz-derived mDAP receptor)
creator: Daniel Wedemeyer, Alp Mirdoğan
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T10:47:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming as soon as we validated mTaz.
mutable notes: meso-2,6-Diaminopimelic acid (mDAP) is a non-proteinogenic amino acid that is synthesized and se-creted by Chlamydia trachomatis. mDAP has a relatively high chemical similarity to aspartate, sharing a Tanimoto coefficient of T=0.8 in a molecular fingerprint analysis [T<0: chemically very different struc-tures; T=1: identity]. As a proof of concept, a molecular docking model proposes few mutations which would change the Tar specificity towards mDAP. As input for molecular docking we used the crys-tal structure of the ligand binding domain of Tar bound to aspartate (PDB: 4Z9H). As a base for the part sequence, the BioBrick representation of the original Taz, BBa_C0082, was used. The BBa_C0082 BioBrick features a small part of the vector (64 nucleotides) it was derived from on its 3??? end, which we have removed along with the stop codon inside the Part for further fusion protein de-sign in RFC 12, 21, 23 and 25 assembly standards. We used the Glide algorithm, Maestro and the Schrodinger Suite for molecular docking of aspartate and mDAP to the Tar ligand binding domain (PDB: 4Z9H). Because of its structural similarity to aspartate, mDAP showed some affinity to the native Tar aspartate binding site (Table 1). Since mDAP is larger than aspartate, Y149 led to structural interference, not allowing mDAP to fully enter the binding site. To create the additional space needed for mDAP binding, we substituted Y149 with smaller amino acids. The best results were obtained with serine. Because of the additional size of mDAP, the bigger challenge was to reduce the affinity of aspartate rather than to raise the affinity of mDAP. We identified R64 as a key residue for aspartate binding which hovewer was not needed to bind mDAP. To minimally interfere with folding and function we performed a constitutive substitution and substituted it with lysine, creating the R64K Y149S double mutant called mTaz hereafter. Using mTaz, a higher docking score was achieved for mDAP than for aspartate (…
mutable provenance: mTaz is based on the Tar-EnvZ chimeric receptor which is capable of detecting aspartate in the medium as described by Utsumi et al. 1989. Tar, or methyl-accepting chemotaxis protein II, is a signal transducer in Escherichia coli chemotaxis and is activated upon the recognition of aspartate. Tar is sensitive to mutations altering its ligand specificity which sometimes results in recognizing other amino acids. EnvZ is a part of the well-studied and commonly used two-component system EnvZ/OmpR in E. coli, offering a simple way of signal transduction. Its periplasmic domain was shown to be exchangeable with different sensor domains e.g. Tar/EnvZ chimeric receptor capable of detecting aspartate in the medium.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: mTaz
sequence annotation: R64K
sequence annotation: Tar
sequence annotation: Y149S
sequence: https://synbiohub.org/public/igem/BBa_K1909002_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2506922
display id: annotation2506922
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506922/range2506922/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506922/range2506922/1
type: Range
persistent identity: range2506922
display id: range2506922
version: 1
direction: direction/forward
top level: mTaz
start: 766
end: 1452
orientation: inline

mTaz
type: SequenceAnnotation
persistent identity: annotation2506915
display id: annotation2506915
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506915/range2506915/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506915/range2506915/1
type: Range
persistent identity: range2506915
display id: range2506915
version: 1
direction: direction/forward
top level: mTaz
start: 1
end: 1452
orientation: inline

R64K
type: SequenceAnnotation
persistent identity: annotation2506923
display id: annotation2506923
version: 1
top level: mTaz
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506923/range2506923/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506923/range2506923/1
type: Range
persistent identity: range2506923
display id: range2506923
version: 1
direction: direction/forward
top level: mTaz
start: 190
end: 192
orientation: inline

Tar
type: SequenceAnnotation
persistent identity: annotation2506919
display id: annotation2506919
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506919/range2506919/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506919/range2506919/1
type: Range
persistent identity: range2506919
display id: range2506919
version: 1
direction: direction/forward
top level: mTaz
start: 1
end: 765
orientation: inline

Y149S
type: SequenceAnnotation
persistent identity: annotation2506924
display id: annotation2506924
version: 1
top level: mTaz
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506924/range2506924/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506924/range2506924/1
type: Range
persistent identity: range2506924
display id: range2506924
version: 1
direction: direction/forward
top level: mTaz
start: 445
end: 447
orientation: inline

BBa_K216003
type: ComponentDefinition
persistent identity: BBa K216003
display id: BBa_K216003
version: 1
was generated by: iGEM to SBOL conversion
description: TNT receptor TNT-R3
creator: Edinburgh iGEM 2009
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: TNT-R3: a computationally designed receptor binding TNT, made by mutation of the Escherichia coli periplasmic ribose-binding protein: see Looger, L., Dwyer, M.A., Smit, J.J., and Wellinga, H.W. 2003. Computational design of receptor proteins with novel functions. Nature 423, 185-190. This receptor interacts with a hybrid transmembrane protein Trz, created by combining the normal partner of ribose-binding-protein, Trg, with the intracellular part of 2-component sensor protein EnvZ, which normally phosphorylates response regulator protein OmpR, allowing it to activate promoters such as PompC (see Baumgartner, J.W., Kim, C., Brisette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg thate recognizes sugar-binding proteins to the kinase/phosphatase domainof Osmosensor EnvZ. J. Bacteriol. 176, 1157-1163). To use this receptor, include it in a system with Trz and a reporter gene controlled by PompC.To avoid cross-talk, it may be necessary to use a chassis lacking EnvZ and ribose-binding protein.
mutable notes: No special considerations.
mutable provenance: Derived by mutagenesis of the Escherichia coli periplasmic ribose-binding protein: see Looger, L., Dwyer, M.A., Smit, J.J., and Wellinga, H.W. 2003. Computational design of receptor proteins with novel functions. Nature 423, 185-190.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: START
sequence annotation: RBS
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K216003_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2027065
display id: annotation2027065
version: 1
top level: BBa_K216003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027065/range2027065/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027065/range2027065/1
type: Range
persistent identity: range2027065
display id: range2027065
version: 1
direction: direction/forward
top level: BBa_K216003
start: 12
end: 14
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2027064
display id: annotation2027064
version: 1
top level: BBa_K216003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027064/range2027064/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027064/range2027064/1
type: Range
persistent identity: range2027064
display id: range2027064
version: 1
direction: direction/forward
top level: BBa_K216003
start: 1
end: 7
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027066
display id: annotation2027066
version: 1
top level: BBa_K216003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216003/annotation2027066/range2027066/1

https://synbiohub.org/public/igem/BBa_K216003/annotation2027066/range2027066/1
type: Range
persistent identity: range2027066
display id: range2027066
version: 1
direction: direction/forward
top level: BBa_K216003
start: 846
end: 851
orientation: inline

CBPa-BpA
type: ComponentDefinition
persistent identity: BBa K2053001
display id: BBa_K2053001
version: 1
was generated by: iGEM to SBOL conversion
description: Fusion cellulose-binding domain of CBP-B domain of protein A
creator: Mathieu Hubert
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T01:01:26Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2521_
m user id: 31618
owner id: 31618
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: It is a fusion protein between the Clostridium cellulovorans cellulose-binding domain of cellulose-binding protein A (CBPa) with the B domain of staphylococcus aureus protein A (BpA). This fusion protein is used to bind antibodies to cellulose. We use these functions to functionalize a cellulose-based patch into an immunodetection-based system.
mutable notes: Polyhistidine tag (6 His) sequence was added at the 5'end as well as pET43.1a-derived Lac operator, ribosome-binding site (RBS), and T7 terminator.
mutable provenance: Cellulose-binding domain of cellulose-binding protein A was taken from iGEM Bielefeld-Germany 2012 team (BBa_K863110) and B domain of protein A was taken from iGEM Warsaw 2008 (BBa_K103003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Start codon
sequence annotation: TEV cleavage site
sequence annotation: BpA
sequence annotation: pET43.1a
sequence annotation: Stop codon
sequence annotation: RBS
sequence annotation: Terminator T7
sequence annotation: CBPa
sequence annotation: Linker
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K2053001_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2492997
display id: annotation2492997
version: 1
top level: CBPa-BpA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2492997/range2492997/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2492997/range2492997/1
type: Range
persistent identity: range2492997
display id: range2492997
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 37
end: 39
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2492999
display id: annotation2492999
version: 1
top level: CBPa-BpA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2492999/range2492999/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2492999/range2492999/1
type: Range
persistent identity: range2492999
display id: range2492999
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 58
end: 78
orientation: inline

BpA
type: SequenceAnnotation
persistent identity: annotation2493002
display id: annotation2493002
version: 1
top level: CBPa-BpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493002/range2493002/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493002/range2493002/1
type: Range
persistent identity: range2493002
display id: range2493002
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 421
end: 642
orientation: inline

pET43.1a
type: SequenceAnnotation
persistent identity: annotation2492995
display id: annotation2492995
version: 1
top level: CBPa-BpA
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2492995/range2492995/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2492995/range2492995/1
type: Range
persistent identity: range2492995
display id: range2492995
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 1
end: 36
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2493004
display id: annotation2493004
version: 1
top level: CBPa-BpA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493004/range2493004/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493004/range2493004/1
type: Range
persistent identity: range2493004
display id: range2493004
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 646
end: 648
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2493003
display id: annotation2493003
version: 1
top level: CBPa-BpA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493003/range2493003/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493003/range2493003/1
type: Range
persistent identity: range2493003
display id: range2493003
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 643
end: 645
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2492996
display id: annotation2492996
version: 1
top level: CBPa-BpA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2492996/range2492996/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2492996/range2492996/1
type: Range
persistent identity: range2492996
display id: range2492996
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 23
end: 28
orientation: inline

Terminator T7
type: SequenceAnnotation
persistent identity: annotation2493005
display id: annotation2493005
version: 1
top level: CBPa-BpA
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493005/range2493005/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493005/range2493005/1
type: Range
persistent identity: range2493005
display id: range2493005
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 649
end: 695
orientation: inline

CBPa
type: SequenceAnnotation
persistent identity: annotation2493000
display id: annotation2493000
version: 1
top level: CBPa-BpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493000/range2493000/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493000/range2493000/1
type: Range
persistent identity: range2493000
display id: range2493000
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 79
end: 384
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2493001
display id: annotation2493001
version: 1
top level: CBPa-BpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2493001/range2493001/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2493001/range2493001/1
type: Range
persistent identity: range2493001
display id: range2493001
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 385
end: 420
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2492998
display id: annotation2492998
version: 1
top level: CBPa-BpA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2053001/annotation2492998/range2492998/1

https://synbiohub.org/public/igem/BBa_K2053001/annotation2492998/range2492998/1
type: Range
persistent identity: range2492998
display id: range2492998
version: 1
direction: direction/forward
top level: CBPa-BpA
start: 40
end: 57
orientation: inline

BBa_K133062
type: ComponentDefinition
persistent identity: BBa K133062
display id: BBa_K133062
version: 1
was generated by: iGEM to SBOL conversion
description: gyrHP
creator: Jan Lonzaric
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 3358
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133062_sequence/1

BBa_K1909007
type: ComponentDefinition
persistent identity: BBa K1909007
display id: BBa_K1909007
version: 1
was generated by: iGEM to SBOL conversion
description: Strong Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:41:12Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 0
m user id: 29525
owner id: 29525
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K081005
component: BBa_K1909002
sequence annotation: BBa_K1909002
sequence annotation: BBa_K081005
sequence: https://synbiohub.org/public/igem/BBa_K1909007_sequence/1

BBa_K081005
type: Component
persistent identity: component2507545
display id: component2507545
version: 1
top level: BBa_K1909007
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_K1909002
type: Component
persistent identity: component2507551
display id: component2507551
version: 1
top level: BBa_K1909007
access: public
definition: mTaz

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2507551
display id: annotation2507551
version: 1
top level: BBa_K1909007
location: https://synbiohub.org/public/igem/BBa_K1909007/annotation2507551/range2507551/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909007/annotation2507551/range2507551/1
type: Range
persistent identity: range2507551
display id: range2507551
version: 1
direction: direction/reverse
top level: BBa_K1909007
start: 65
end: 1516
orientation: inline

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2507545
display id: annotation2507545
version: 1
top level: BBa_K1909007
location: https://synbiohub.org/public/igem/BBa_K1909007/annotation2507545/range2507545/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K1909007/annotation2507545/range2507545/1
type: Range
persistent identity: range2507545
display id: range2507545
version: 1
direction: direction/reverse
top level: BBa_K1909007
start: 1
end: 58
orientation: inline

BBa_K211001
type: ComponentDefinition
persistent identity: BBa K211001
display id: BBa_K211001
version: 1
was generated by: iGEM to SBOL conversion
description: RI7
creator: ZHONG Wanting
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _313_
m user id: 0
owner id: 4277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complete CDS of Rat olfactory protein mRNA, clone I7.
mutable provenance: Rattus norvegicus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K211001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2035780
display id: annotation2035780
version: 1
top level: BBa_K211001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K211001/annotation2035780/range2035780/1

https://synbiohub.org/public/igem/BBa_K211001/annotation2035780/range2035780/1
type: Range
persistent identity: range2035780
display id: range2035780
version: 1
direction: direction/forward
top level: BBa_K211001
start: 982
end: 987
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2035779
display id: annotation2035779
version: 1
top level: BBa_K211001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K211001/annotation2035779/range2035779/1

https://synbiohub.org/public/igem/BBa_K211001/annotation2035779/range2035779/1
type: Range
persistent identity: range2035779
display id: range2035779
version: 1
direction: direction/forward
top level: BBa_K211001
start: 1
end: 3
orientation: inline

BBa_K772001
type: ComponentDefinition
persistent identity: BBa K772001
display id: BBa_K772001
version: 1
was generated by: iGEM to SBOL conversion
description: EpCAM Binding Dock: C-terminus
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
mutable description: This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
star: false
creator: Mustafa Elitok
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Binding Dock
sequence annotation: CD215 Antibody
sequence annotation: OmpA (Dock)
sequence annotation: RBS
sequence annotation: TEV Protease C terminus
sequence annotation: pCons Promoter
sequence: https://synbiohub.org/public/igem/BBa_K772001_sequence/1

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203674
display id: annotation2203674
version: 1
top level: BBa_K772001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1
type: Range
persistent identity: range2203674
display id: range2203674
version: 1
top level: BBa_K772001
direction: direction/forward
start: 94
end: 1159
orientation: inline

CD215 Antibody
type: SequenceAnnotation
persistent identity: annotation2203673
display id: annotation2203673
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1
type: Range
persistent identity: range2203673
display id: range2203673
version: 1
top level: BBa_K772001
direction: direction/forward
start: 858
end: 1159
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203671
display id: annotation2203671
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1
type: Range
persistent identity: range2203671
display id: range2203671
version: 1
top level: BBa_K772001
direction: direction/forward
start: 420
end: 857
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203670
display id: annotation2203670
version: 1
top level: BBa_K772001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1
type: Range
persistent identity: range2203670
display id: range2203670
version: 1
top level: BBa_K772001
direction: direction/forward
start: 73
end: 93
orientation: inline

TEV Protease C terminus
type: SequenceAnnotation
persistent identity: annotation2203672
display id: annotation2203672
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1
type: Range
persistent identity: range2203672
display id: range2203672
version: 1
top level: BBa_K772001
direction: direction/forward
start: 94
end: 419
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203669
display id: annotation2203669
version: 1
top level: BBa_K772001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1
type: Range
persistent identity: range2203669
display id: range2203669
version: 1
top level: BBa_K772001
direction: direction/forward
start: 1
end: 72
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor (expressed)
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23110
component: BBa_K389001
component: BBa_B0034
sequence annotation: BBa_K389001
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
access: public
definition: virA

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
direction: direction/reverse
top level: virA
start: 62
end: 2566
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
direction: direction/reverse
top level: virA
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
direction: direction/reverse
top level: virA
start: 44
end: 55
orientation: inline

BBa_I721002
type: ComponentDefinition
persistent identity: BBa I721002
display id: BBa_I721002
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Binding Protein
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lead binding protein part 3
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I721002_sequence/1

BBa_K772100
type: ComponentDefinition
persistent identity: BBa K772100
display id: BBa_K772100
version: 1
was generated by: iGEM to SBOL conversion
description: EpCAM Detection Device
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
mutable description: This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease.
star: false
creator: Mustafa Elitok
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K772002
component: BBa_K772001
sequence annotation: BBa_K772002
sequence annotation: BBa_K772001
sequence: https://synbiohub.org/public/igem/BBa_K772100_sequence/1

BBa_K772002
type: Component
persistent identity: component2211348
display id: component2211348
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772002

BBa_K772001
type: Component
persistent identity: component2211341
display id: component2211341
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772001

BBa_K772002
type: SequenceAnnotation
persistent identity: annotation2211348
display id: annotation2211348
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
component: BBa_K772002

https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
type: Range
persistent identity: range2211348
display id: range2211348
version: 1
top level: BBa_K772100
direction: direction/reverse
start: 1168
end: 2305
orientation: inline

BBa_K772001
type: SequenceAnnotation
persistent identity: annotation2211341
display id: annotation2211341
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
component: BBa_K772001

https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
type: Range
persistent identity: range2211341
display id: range2211341
version: 1
top level: BBa_K772100
direction: direction/reverse
start: 1
end: 1159
orientation: inline

BBa_K909009
type: ComponentDefinition
persistent identity: BBa K909009
display id: BBa_K909009
version: 1
was generated by: iGEM to SBOL conversion
description: cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain.
mutable notes: Contains two illegal PstI sites.
mutable provenance: cDNA of UVR8 from Arabidopsis thaliana was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BamHI site
sequence: https://synbiohub.org/public/igem/BBa_K909009_sequence/1

BamHI site
type: SequenceAnnotation
persistent identity: annotation2202567
display id: annotation2202567
version: 1
top level: BBa_K909009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1

https://synbiohub.org/public/igem/BBa_K909009/annotation2202567/range2202567/1
type: Range
persistent identity: range2202567
display id: range2202567
version: 1
direction: direction/forward
top level: BBa_K909009
start: 4
end: 9
orientation: inline

BBa_K1909008
type: ComponentDefinition
persistent identity: BBa K1909008
display id: BBa_K1909008
version: 1
was generated by: iGEM to SBOL conversion
description: Medium Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:44:03Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K1909002
component: BBa_K608006
sequence annotation: BBa_K1909002
sequence annotation: BBa_K608006
sequence: https://synbiohub.org/public/igem/BBa_K1909008_sequence/1

BBa_K1909002
type: Component
persistent identity: component2507597
display id: component2507597
version: 1
top level: BBa_K1909008
access: public
definition: mTaz

BBa_K608006
type: Component
persistent identity: component2507591
display id: component2507591
version: 1
top level: BBa_K1909008
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2507597
display id: annotation2507597
version: 1
top level: BBa_K1909008
location: https://synbiohub.org/public/igem/BBa_K1909008/annotation2507597/range2507597/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909008/annotation2507597/range2507597/1
type: Range
persistent identity: range2507597
display id: range2507597
version: 1
direction: direction/reverse
top level: BBa_K1909008
start: 63
end: 1514
orientation: inline

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2507591
display id: annotation2507591
version: 1
top level: BBa_K1909008
location: https://synbiohub.org/public/igem/BBa_K1909008/annotation2507591/range2507591/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1909008/annotation2507591/range2507591/1
type: Range
persistent identity: range2507591
display id: range2507591
version: 1
direction: direction/reverse
top level: BBa_K1909008
start: 1
end: 56
orientation: inline

BBa_K772002
type: ComponentDefinition
persistent identity: BBa K772002
display id: BBa_K772002
version: 1
was generated by: iGEM to SBOL conversion
description: EpCAM Binding Dock: N terminus
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
mutable description: This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
star: false
creator: Mustafa Elitok
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: TEV Protease N terminus
sequence annotation: OmpA (Dock)
sequence annotation: CD215 antibody
sequence annotation: pCons Promoter
sequence annotation: Binding Dock
sequence: https://synbiohub.org/public/igem/BBa_K772002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203683
display id: annotation2203683
version: 1
top level: BBa_K772002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1
type: Range
persistent identity: range2203683
display id: range2203683
version: 1
top level: BBa_K772002
direction: direction/forward
start: 73
end: 93
orientation: inline

TEV Protease N terminus
type: SequenceAnnotation
persistent identity: annotation2203685
display id: annotation2203685
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1
type: Range
persistent identity: range2203685
display id: range2203685
version: 1
top level: BBa_K772002
direction: direction/forward
start: 94
end: 398
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203682
display id: annotation2203682
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1
type: Range
persistent identity: range2203682
display id: range2203682
version: 1
top level: BBa_K772002
direction: direction/forward
start: 399
end: 836
orientation: inline

CD215 antibody
type: SequenceAnnotation
persistent identity: annotation2203686
display id: annotation2203686
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1
type: Range
persistent identity: range2203686
display id: range2203686
version: 1
top level: BBa_K772002
direction: direction/forward
start: 837
end: 1138
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203681
display id: annotation2203681
version: 1
top level: BBa_K772002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1
type: Range
persistent identity: range2203681
display id: range2203681
version: 1
top level: BBa_K772002
direction: direction/forward
start: 1
end: 72
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203684
display id: annotation2203684
version: 1
top level: BBa_K772002
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1
type: Range
persistent identity: range2203684
display id: range2203684
version: 1
top level: BBa_K772002
direction: direction/forward
start: 94
end: 1138
orientation: inline

BBa_K216002
type: ComponentDefinition
persistent identity: BBa K216002
display id: BBa_K216002
version: 1
was generated by: iGEM to SBOL conversion
description: TNT Receptor TNT-R1
creator: Edinburgh iGEM 2009
created: 2009-08-03T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 5058
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: TNT.R1 has fair affinity (340nM) for trinitrotoluene (TNT)that is not a naturally occurring compound. TNT.R1 is less thermostable than the wildtype receptor. As such, it is suggested that the receptor be used shortly after expression. TNT receptor 1 (TNT.R1) is a computationally-derived ligand receptor specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that the receptor recognizes TNT instead of the wild-type ligand.
mutable notes: This part is cloned into BioBrick vector pSB1A2.
mutable provenance: Computationally-derived ligand receptor. Looger, L. L., Dwyer, M. A., Smith, J. J. and Hellinga, H. W. (2003). Computational design of receptor and sensor proteins with novel functions. Nature 423: 185 - 190.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: START
sequence annotation: STOP
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K216002_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2027070
display id: annotation2027070
version: 1
top level: BBa_K216002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216002/annotation2027070/range2027070/1

https://synbiohub.org/public/igem/BBa_K216002/annotation2027070/range2027070/1
type: Range
persistent identity: range2027070
display id: range2027070
version: 1
direction: direction/forward
top level: BBa_K216002
start: 12
end: 14
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2027071
display id: annotation2027071
version: 1
top level: BBa_K216002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216002/annotation2027071/range2027071/1

https://synbiohub.org/public/igem/BBa_K216002/annotation2027071/range2027071/1
type: Range
persistent identity: range2027071
display id: range2027071
version: 1
direction: direction/forward
top level: BBa_K216002
start: 846
end: 851
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2014755
display id: annotation2014755
version: 1
top level: BBa_K216002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216002/annotation2014755/range2014755/1

https://synbiohub.org/public/igem/BBa_K216002/annotation2014755/range2014755/1
type: Range
persistent identity: range2014755
display id: range2014755
version: 1
direction: direction/forward
top level: BBa_K216002
start: 1
end: 6
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389001
display id: BBa_K389001
version: 1
was generated by: iGEM to SBOL conversion
description: VirA receptor
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis.
mutable notes: Illegal ''PstI'' restriction site removed
mutable provenance: ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Illegal PstI site removed
sequence annotation: virA
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389001_sequence/1

Illegal PstI site removed
type: SequenceAnnotation
persistent identity: annotation2077043
display id: annotation2077043
version: 1
top level: virA
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
type: Range
persistent identity: range2077043
display id: range2077043
version: 1
direction: direction/forward
top level: virA
start: 187
end: 187
orientation: inline

virA
type: SequenceAnnotation
persistent identity: annotation2077044
display id: annotation2077044
version: 1
top level: virA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
type: Range
persistent identity: range2077044
display id: range2077044
version: 1
direction: direction/forward
top level: virA
start: 1
end: 2505
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078363
display id: annotation2078363
version: 1
top level: virA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
type: Range
persistent identity: range2078363
display id: range2078363
version: 1
direction: direction/forward
top level: virA
start: 2503
end: 2505
orientation: inline

BBa_K1405004
type: ComponentDefinition
persistent identity: BBa K1405004
display id: BBa_K1405004
version: 1
was generated by: iGEM to SBOL conversion
description: McfR
creator: Xuan Wang, Shu Zhang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1783_
m user id: 0
owner id: 20328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Background McfR is an organic acid responsive chemoreceptor originally found in***. BBa_K1405004 contains an insulator, RBS sequence, and McfR coding sequence and its expression is under the control of the constitutive promoter BBa_J23100. To be continued.
mutable notes: To be continued.
mutable provenance: To be continued.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J23100
sequence annotation: insulator
sequence annotation: RBS
sequence annotation: McfR
sequence: https://synbiohub.org/public/igem/BBa_K1405004_sequence/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2416937
display id: annotation2416937
version: 1
top level: BBa_K1405004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1405004/annotation2416937/range2416937/1

https://synbiohub.org/public/igem/BBa_K1405004/annotation2416937/range2416937/1
type: Range
persistent identity: range2416937
display id: range2416937
version: 1
direction: direction/forward
top level: BBa_K1405004
start: 1
end: 35
orientation: inline

insulator
type: SequenceAnnotation
persistent identity: annotation2416938
display id: annotation2416938
version: 1
top level: BBa_K1405004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1405004/annotation2416938/range2416938/1

https://synbiohub.org/public/igem/BBa_K1405004/annotation2416938/range2416938/1
type: Range
persistent identity: range2416938
display id: range2416938
version: 1
direction: direction/forward
top level: BBa_K1405004
start: 36
end: 50
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2416939
display id: annotation2416939
version: 1
top level: BBa_K1405004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1405004/annotation2416939/range2416939/1

https://synbiohub.org/public/igem/BBa_K1405004/annotation2416939/range2416939/1
type: Range
persistent identity: range2416939
display id: range2416939
version: 1
direction: direction/forward
top level: BBa_K1405004
start: 51
end: 70
orientation: inline

McfR
type: SequenceAnnotation
persistent identity: annotation2416940
display id: annotation2416940
version: 1
top level: BBa_K1405004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1405004/annotation2416940/range2416940/1

https://synbiohub.org/public/igem/BBa_K1405004/annotation2416940/range2416940/1
type: Range
persistent identity: range2416940
display id: range2416940
version: 1
direction: direction/forward
top level: BBa_K1405004
start: 71
end: 1696
orientation: inline

BBa_K1909011
type: ComponentDefinition
persistent identity: BBa K1909011
display id: BBa_K1909011
version: 1
was generated by: iGEM to SBOL conversion
description: Medium Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:45:57Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0082
component: BBa_K608006
sequence annotation: BBa_C0082
sequence annotation: BBa_K608006
sequence: https://synbiohub.org/public/igem/BBa_K1909011_sequence/1

BBa_C0082
type: Component
persistent identity: component2508789
display id: component2508789
version: 1
top level: BBa_K1909011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0082/1

BBa_K608006
type: Component
persistent identity: component2508783
display id: component2508783
version: 1
top level: BBa_K1909011
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508789
display id: annotation2508789
version: 1
top level: BBa_K1909011
location: https://synbiohub.org/public/igem/BBa_K1909011/annotation2508789/range2508789/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909011/annotation2508789/range2508789/1
type: Range
persistent identity: range2508789
display id: range2508789
version: 1
direction: direction/reverse
top level: BBa_K1909011
start: 63
end: 1578
orientation: inline

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2508783
display id: annotation2508783
version: 1
top level: BBa_K1909011
location: https://synbiohub.org/public/igem/BBa_K1909011/annotation2508783/range2508783/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1909011/annotation2508783/range2508783/1
type: Range
persistent identity: range2508783
display id: range2508783
version: 1
direction: direction/reverse
top level: BBa_K1909011
start: 1
end: 56
orientation: inline

BBa_K211002
type: ComponentDefinition
persistent identity: BBa K211002
display id: BBa_K211002
version: 1
was generated by: iGEM to SBOL conversion
description: RI7-odr10 chimeric GPCR
creator: ZHONG Wanting
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _313_
m user id: 0
owner id: 4277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The chimeric GPCR consists of the N- and C- terminals of the rat OR RI7, flanking the TM2-TM7 ligand-binding domain of the c. elegans OR odr-10. It can be functionally expressed in S. cerevisiae, activating the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.
mutable provenance: C. elegans, Rattus norvegicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Gasubunit coupling sequence
sequence annotation: start
sequence annotation: membrane localization sequence
sequence annotation: diacetyl sensing region
sequence: https://synbiohub.org/public/igem/BBa_K211002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2035762
display id: annotation2035762
version: 1
top level: BBa_K211002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1
type: Range
persistent identity: range2035762
display id: range2035762
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1057
end: 1062
orientation: inline

Gasubunit coupling sequence
type: SequenceAnnotation
persistent identity: annotation2049259
display id: annotation2049259
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1
type: Range
persistent identity: range2049259
display id: range2049259
version: 1
direction: direction/forward
top level: BBa_K211002
start: 958
end: 1062
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2035761
display id: annotation2035761
version: 1
top level: BBa_K211002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1
type: Range
persistent identity: range2035761
display id: range2035761
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 3
orientation: inline

membrane localization sequence
type: SequenceAnnotation
persistent identity: annotation2049258
display id: annotation2049258
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1
type: Range
persistent identity: range2049258
display id: range2049258
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 183
orientation: inline

diacetyl sensing region
type: SequenceAnnotation
persistent identity: annotation2049260
display id: annotation2049260
version: 1
top level: BBa_K211002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1
type: Range
persistent identity: range2049260
display id: range2049260
version: 1
direction: direction/forward
top level: BBa_K211002
start: 184
end: 957
orientation: inline

DigA
type: ComponentDefinition
persistent identity: BBa K243003
display id: BBa_K243003
version: 1
was generated by: iGEM to SBOL conversion
description: Digoxigenin binding protein (DigA)
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: synthesized by purimex
owned by: james
owned by: myers
mutable description: Digoxigenin is a steroid extracted from the plant Digitalis purpurea and D.lanata. Digoxigenin modified oligonucleotides are widely used as high sensitivity probes for non-radioactive immunoassay s and hybridization experiments. These DIG-probes are monitored by anti-DIG antibodies. These antibodies only show cross-reactivity with blossoms and leaves of D. spec(sole natural sources for DIG) ??? so they are suitable for analysis of any other biological species. Anti-DIG antibodies are either modified with fluorescent dyes (direct detection) or with an enzyme (indirect detection via enzyme-substrate reaction).
discontinued: false
dominant: false
experience: experience/Works
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Digoxigenin tag
sequence: https://synbiohub.org/public/igem/BBa_K243003_sequence/1

Digoxigenin tag
type: SequenceAnnotation
persistent identity: annotation2041878
display id: annotation2041878
version: 1
top level: DigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K243003/annotation2041878/range2041878/1

https://synbiohub.org/public/igem/BBa_K243003/annotation2041878/range2041878/1
type: Range
persistent identity: range2041878
display id: range2041878
version: 1
top level: DigA
direction: direction/forward
start: 1
end: 552
orientation: inline

BBa_K2056000
type: ComponentDefinition
persistent identity: BBa K2056000
display id: BBa_K2056000
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive promoter(J23110), RBS(B0034), NsrR(K2056001), Double terminator(B0015)
creator: Muhammad Ismail
created: 2016-10-11T11:00:00Z
modified: 2016-10-19T01:27:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: lorem ipsum falana dhimaka
mutable notes: Change of RBS and reporter device
mutable provenance: Bio- Brick Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_J23110
component: BBa_K2056001
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_B0015
sequence annotation: BBa_K2056001
sequence: https://synbiohub.org/public/igem/BBa_K2056000_sequence/1

BBa_B0015
type: Component
persistent identity: component2520574
display id: component2520574
version: 1
top level: BBa_K2056000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2520566
display id: component2520566
version: 1
top level: BBa_K2056000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: Component
persistent identity: component2520564
display id: component2520564
version: 1
top level: BBa_K2056000
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K2056001
type: Component
persistent identity: component2520567
display id: component2520567
version: 1
top level: BBa_K2056000
access: public
definition: https://synbiohub.org/public/igem/BBa_K2056001/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2520566
display id: annotation2520566
version: 1
top level: BBa_K2056000
location: https://synbiohub.org/public/igem/BBa_K2056000/annotation2520566/range2520566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2056000/annotation2520566/range2520566/1
type: Range
persistent identity: range2520566
display id: range2520566
version: 1
direction: direction/reverse
top level: BBa_K2056000
start: 44
end: 55
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2520564
display id: annotation2520564
version: 1
top level: BBa_K2056000
location: https://synbiohub.org/public/igem/BBa_K2056000/annotation2520564/range2520564/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K2056000/annotation2520564/range2520564/1
type: Range
persistent identity: range2520564
display id: range2520564
version: 1
direction: direction/reverse
top level: BBa_K2056000
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520574
display id: annotation2520574
version: 1
top level: BBa_K2056000
location: https://synbiohub.org/public/igem/BBa_K2056000/annotation2520574/range2520574/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2056000/annotation2520574/range2520574/1
type: Range
persistent identity: range2520574
display id: range2520574
version: 1
direction: direction/reverse
top level: BBa_K2056000
start: 498
end: 626
orientation: inline

BBa_K2056001
type: SequenceAnnotation
persistent identity: annotation2520567
display id: annotation2520567
version: 1
top level: BBa_K2056000
location: https://synbiohub.org/public/igem/BBa_K2056000/annotation2520567/range2520567/1
component: BBa_K2056001

https://synbiohub.org/public/igem/BBa_K2056000/annotation2520567/range2520567/1
type: Range
persistent identity: range2520567
display id: range2520567
version: 1
direction: direction/reverse
top level: BBa_K2056000
start: 64
end: 489
orientation: inline

BBa_K1909003
type: ComponentDefinition
persistent identity: BBa K1909003
display id: BBa_K1909003
version: 1
was generated by: iGEM to SBOL conversion
description: EnvZ Signal Peptide and first Transmembrane Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:06:33Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: For EnvZ fusion protein design. Long description will be added soon.
mutable notes: coming soon
mutable provenance: Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1909003_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507125
display id: annotation2507125
version: 1
top level: BBa_K1909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1
type: Range
persistent identity: range2507125
display id: range2507125
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 1
end: 161
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2507149
display id: annotation2507149
version: 1
top level: BBa_K1909003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1
type: Range
persistent identity: range2507149
display id: range2507149
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 46
end: 69
orientation: inline

BBa_K1909009
type: ComponentDefinition
persistent identity: BBa K1909009
display id: BBa_K1909009
version: 1
was generated by: iGEM to SBOL conversion
description: Weak Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:43:16Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K608007
component: BBa_K1909002
sequence annotation: BBa_K608007
sequence annotation: BBa_K1909002
sequence: https://synbiohub.org/public/igem/BBa_K1909009_sequence/1

BBa_K608007
type: Component
persistent identity: component2508760
display id: component2508760
version: 1
top level: BBa_K1909009
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1909002
type: Component
persistent identity: component2508766
display id: component2508766
version: 1
top level: BBa_K1909009
access: public
definition: mTaz

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2508760
display id: annotation2508760
version: 1
top level: BBa_K1909009
location: https://synbiohub.org/public/igem/BBa_K1909009/annotation2508760/range2508760/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1909009/annotation2508760/range2508760/1
type: Range
persistent identity: range2508760
display id: range2508760
version: 1
direction: direction/reverse
top level: BBa_K1909009
start: 1
end: 57
orientation: inline

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2508766
display id: annotation2508766
version: 1
top level: BBa_K1909009
location: https://synbiohub.org/public/igem/BBa_K1909009/annotation2508766/range2508766/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909009/annotation2508766/range2508766/1
type: Range
persistent identity: range2508766
display id: range2508766
version: 1
direction: direction/reverse
top level: BBa_K1909009
start: 64
end: 1515
orientation: inline

BBa_K103003
type: ComponentDefinition
persistent identity: BBa K103003
display id: BBa_K103003
version: 1
was generated by: iGEM to SBOL conversion
description: B domain of Staphylococcal protein A
creator: Michael Lower
created: 2008-09-13T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: protein A from Staphylococcus aureus. Also known as part of Tap-Tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NdeI
sequence annotation: ATG
sequence annotation: SacI
sequence annotation: B-domain of protein A
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_K103003_sequence/1

NdeI
type: SequenceAnnotation
persistent identity: annotation1989822
display id: annotation1989822
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1989822/range1989822/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1989822/range1989822/1
type: Range
persistent identity: range1989822
display id: range1989822
version: 1
direction: direction/forward
top level: BBa_K103003
start: 3
end: 8
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1975845
display id: annotation1975845
version: 1
top level: BBa_K103003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1975845/range1975845/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1975845/range1975845/1
type: Range
persistent identity: range1975845
display id: range1975845
version: 1
direction: direction/forward
top level: BBa_K103003
start: 6
end: 9
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1989823
display id: annotation1989823
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1989823/range1989823/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1989823/range1989823/1
type: Range
persistent identity: range1989823
display id: range1989823
version: 1
direction: direction/forward
top level: BBa_K103003
start: 230
end: 235
orientation: inline

B-domain of protein A
type: SequenceAnnotation
persistent identity: annotation1975846
display id: annotation1975846
version: 1
top level: BBa_K103003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1975846/range1975846/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1975846/range1975846/1
type: Range
persistent identity: range1975846
display id: range1975846
version: 1
direction: direction/forward
top level: BBa_K103003
start: 6
end: 233
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1986760
display id: annotation1986760
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1986760/range1986760/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1986760/range1986760/1
type: Range
persistent identity: range1986760
display id: range1986760
version: 1
direction: direction/forward
top level: BBa_K103003
start: 9
end: 14
orientation: inline

BBa_K515102
type: ComponentDefinition
persistent identity: BBa K515102
display id: BBa_K515102
version: 1
was generated by: iGEM to SBOL conversion
description: J23100 promoter - PA2652
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A composite part comprising of a J23100 promotor and PA2652 translational unit encoding extracellular chemoreceptor
mutable notes: -
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K515002
sequence annotation: BBa_J23100
sequence annotation: BBa_K515002
sequence: https://synbiohub.org/public/igem/BBa_K515102_sequence/1

BBa_J23100
type: Component
persistent identity: component2127190
display id: component2127190
version: 1
top level: BBa_K515102
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K515002
type: Component
persistent identity: component2127194
display id: component2127194
version: 1
top level: BBa_K515102
access: public
definition: https://synbiohub.org/public/igem/BBa_K515002/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2127190
display id: annotation2127190
version: 1
top level: BBa_K515102
location: https://synbiohub.org/public/igem/BBa_K515102/annotation2127190/range2127190/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515102/annotation2127190/range2127190/1
type: Range
persistent identity: range2127190
display id: range2127190
version: 1
direction: direction/reverse
top level: BBa_K515102
start: 1
end: 35
orientation: inline

BBa_K515002
type: SequenceAnnotation
persistent identity: annotation2127194
display id: annotation2127194
version: 1
top level: BBa_K515102
location: https://synbiohub.org/public/igem/BBa_K515102/annotation2127194/range2127194/1
component: BBa_K515002

https://synbiohub.org/public/igem/BBa_K515102/annotation2127194/range2127194/1
type: Range
persistent identity: range2127194
display id: range2127194
version: 1
direction: direction/reverse
top level: BBa_K515102
start: 36
end: 1759
orientation: inline

EnvZ Tail
type: ComponentDefinition
persistent identity: BBa K1909005
display id: BBa_K1909005
version: 1
was generated by: iGEM to SBOL conversion
description: EnvZ Signal Transduction and Kinase Domains
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: E. coli EnvZ (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence: https://synbiohub.org/public/igem/BBa_K1909005_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507346
display id: annotation2507346
version: 1
top level: EnvZ Tail
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1

https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1
type: Range
persistent identity: range2507346
display id: range2507346
version: 1
direction: direction/forward
top level: EnvZ Tail
start: 1
end: 882
orientation: inline

BBa_K133070
type: ComponentDefinition
persistent identity: BBa K133070
display id: BBa_K133070
version: 1
was generated by: iGEM to SBOL conversion
description: gyrEC
creator: Jan Lonzaric
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 3358
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133070_sequence/1

BBa_K1909012
type: ComponentDefinition
persistent identity: BBa K1909012
display id: BBa_K1909012
version: 1
was generated by: iGEM to SBOL conversion
description: Weak Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:47:22Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0082
component: BBa_K608007
sequence annotation: BBa_K608007
sequence annotation: BBa_C0082
sequence: https://synbiohub.org/public/igem/BBa_K1909012_sequence/1

BBa_C0082
type: Component
persistent identity: component2508813
display id: component2508813
version: 1
top level: BBa_K1909012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0082/1

BBa_K608007
type: Component
persistent identity: component2508807
display id: component2508807
version: 1
top level: BBa_K1909012
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2508807
display id: annotation2508807
version: 1
top level: BBa_K1909012
location: https://synbiohub.org/public/igem/BBa_K1909012/annotation2508807/range2508807/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1909012/annotation2508807/range2508807/1
type: Range
persistent identity: range2508807
display id: range2508807
version: 1
direction: direction/reverse
top level: BBa_K1909012
start: 1
end: 57
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508813
display id: annotation2508813
version: 1
top level: BBa_K1909012
location: https://synbiohub.org/public/igem/BBa_K1909012/annotation2508813/range2508813/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909012/annotation2508813/range2508813/1
type: Range
persistent identity: range2508813
display id: range2508813
version: 1
direction: direction/reverse
top level: BBa_K1909012
start: 64
end: 1579
orientation: inline

BBa_K1909006
type: ComponentDefinition
persistent identity: BBa K1909006
display id: BBa_K1909006
version: 1
was generated by: iGEM to SBOL conversion
description: EnvZ-LRR chimeric receptor for mDAP recognition
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:35:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 0
m user id: 29525
owner id: 29525
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K1909004
component: BBa_K1909005
component: BBa_K1909003
sequence annotation: BBa_K1909005
sequence annotation: BBa_K1909003
sequence annotation: BBa_K1909004
sequence: https://synbiohub.org/public/igem/BBa_K1909006_sequence/1

BBa_K1909004
type: Component
persistent identity: component2507405
display id: component2507405
version: 1
top level: BBa_K1909006
access: public
definition: BBa_K1909004

BBa_K1909005
type: Component
persistent identity: component2507407
display id: component2507407
version: 1
top level: BBa_K1909006
access: public
definition: EnvZ Tail

BBa_K1909003
type: Component
persistent identity: component2507398
display id: component2507398
version: 1
top level: BBa_K1909006
access: public
definition: BBa_K1909003

BBa_K1909005
type: SequenceAnnotation
persistent identity: annotation2507407
display id: annotation2507407
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
component: BBa_K1909005

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
type: Range
persistent identity: range2507407
display id: range2507407
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1143
end: 2024
orientation: inline

BBa_K1909003
type: SequenceAnnotation
persistent identity: annotation2507398
display id: annotation2507398
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
component: BBa_K1909003

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
type: Range
persistent identity: range2507398
display id: range2507398
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1
end: 161
orientation: inline

BBa_K1909004
type: SequenceAnnotation
persistent identity: annotation2507405
display id: annotation2507405
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
component: BBa_K1909004

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
type: Range
persistent identity: range2507405
display id: range2507405
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 168
end: 1136
orientation: inline

BBa_K1909004
type: ComponentDefinition
persistent identity: BBa K1909004
display id: BBa_K1909004
version: 1
was generated by: iGEM to SBOL conversion
description: NOD1 LRR Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:21:00Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: coming soon
mutable provenance: NOD1 (ncbi: https://www.ncbi.nlm.nih.gov/gene/10392)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed CTG to TTG
sequence annotation: Changed CTA to TTA
sequence annotation: NOD1
sequence annotation: Changed GAC to GAT
sequence annotation: Changed CTG to CTA
sequence: https://synbiohub.org/public/igem/BBa_K1909004_sequence/1

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507292
display id: annotation2507292
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1
type: Range
persistent identity: range2507292
display id: range2507292
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 700
end: 702
orientation: inline

Changed CTA to TTA
type: SequenceAnnotation
persistent identity: annotation2507293
display id: annotation2507293
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1
type: Range
persistent identity: range2507293
display id: range2507293
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 718
end: 720
orientation: inline

NOD1
type: SequenceAnnotation
persistent identity: annotation2507199
display id: annotation2507199
version: 1
top level: BBa_K1909004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1
type: Range
persistent identity: range2507199
display id: range2507199
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 1
end: 969
orientation: inline

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507291
display id: annotation2507291
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1
type: Range
persistent identity: range2507291
display id: range2507291
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 277
end: 279
orientation: inline

Changed GAC to GAT
type: SequenceAnnotation
persistent identity: annotation2507200
display id: annotation2507200
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1
type: Range
persistent identity: range2507200
display id: range2507200
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 181
end: 183
orientation: inline

Changed CTG to CTA
type: SequenceAnnotation
persistent identity: annotation2507294
display id: annotation2507294
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1
type: Range
persistent identity: range2507294
display id: range2507294
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 727
end: 729
orientation: inline

BBa_K126000
type: ComponentDefinition
persistent identity: BBa K126000
display id: BBa_K126000
version: 1
was generated by: iGEM to SBOL conversion
description: TE33 Fab L chain
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This antibody was created using mouse hybridomas.
owned by: james
owned by: myers
mutable description: This sequence encodes the amino acid chain of the antigen binding fragment of the TE33 antibody light chain. The antibody is specific for the B subunit of cholera toxin.
discontinued: false
dominant: false
experience: experience/None
group u list: _198_
m user id: 0
owner id: 3233
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: n/a
star: true
creator: Matt Davis
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: variable domain
sequence annotation: constant domain
sequence: https://synbiohub.org/public/igem/BBa_K126000_sequence/1

variable domain
type: SequenceAnnotation
persistent identity: annotation1975587
display id: annotation1975587
version: 1
top level: BBa_K126000
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K126000/annotation1975587/range1975587/1

https://synbiohub.org/public/igem/BBa_K126000/annotation1975587/range1975587/1
type: Range
persistent identity: range1975587
display id: range1975587
version: 1
top level: BBa_K126000
direction: direction/forward
start: 1
end: 342
orientation: inline

constant domain
type: SequenceAnnotation
persistent identity: annotation1975588
display id: annotation1975588
version: 1
top level: BBa_K126000
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K126000/annotation1975588/range1975588/1

https://synbiohub.org/public/igem/BBa_K126000/annotation1975588/range1975588/1
type: Range
persistent identity: range1975588
display id: range1975588
version: 1
top level: BBa_K126000
direction: direction/forward
start: 343
end: 648
orientation: inline

BBa_K1072010
type: ComponentDefinition
persistent identity: BBa K1072010
display id: BBa_K1072010
version: 1
was generated by: iGEM to SBOL conversion
description: Odr-10(diacetyl)GPCR with optimized codon
creator: Junqi kuang, Xihao liao and Jianhui gong
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ.
mutable notes: As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully.
mutable provenance: Originate from the C. elegans.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Odr-10 coding region
sequence: https://synbiohub.org/public/igem/BBa_K1072010_sequence/1

Odr-10 coding region
type: SequenceAnnotation
persistent identity: annotation2357697
display id: annotation2357697
version: 1
top level: BBa_K1072010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1

https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1
type: Range
persistent identity: range2357697
display id: range2357697
version: 1
direction: direction/forward
top level: BBa_K1072010
start: 1
end: 1020
orientation: inline

BBa_K1909010
type: ComponentDefinition
persistent identity: BBa K1909010
display id: BBa_K1909010
version: 1
was generated by: iGEM to SBOL conversion
description: Strong Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:44:31Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0082
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_C0082
sequence: https://synbiohub.org/public/igem/BBa_K1909010_sequence/1

BBa_C0082
type: Component
persistent identity: component2508778
display id: component2508778
version: 1
top level: BBa_K1909010
access: public
definition: https://synbiohub.org/public/igem/BBa_C0082/1

BBa_K081005
type: Component
persistent identity: component2508772
display id: component2508772
version: 1
top level: BBa_K1909010
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2508772
display id: annotation2508772
version: 1
top level: BBa_K1909010
location: https://synbiohub.org/public/igem/BBa_K1909010/annotation2508772/range2508772/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K1909010/annotation2508772/range2508772/1
type: Range
persistent identity: range2508772
display id: range2508772
version: 1
direction: direction/reverse
top level: BBa_K1909010
start: 1
end: 58
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508778
display id: annotation2508778
version: 1
top level: BBa_K1909010
location: https://synbiohub.org/public/igem/BBa_K1909010/annotation2508778/range2508778/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909010/annotation2508778/range2508778/1
type: Range
persistent identity: range2508778
display id: range2508778
version: 1
direction: direction/reverse
top level: BBa_K1909010
start: 65
end: 1580
orientation: inline

https://synbiohub.org/public/igem/BBa_K772001_sequence/1
type: Sequence
persistent identity: BBa K772001 sequence
display id: BBa_K772001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K126000_sequence/1
type: Sequence
persistent identity: BBa K126000 sequence
display id: BBa_K126000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgattgaaactctttacgattgggctagttgacgttttgtgagtagcttcgcaggtatagctattatgtctttcgtattcatcctttgtcaaggttaaggtagatgacatagagtaagttgaatctttactgtcttgatctgtccaagaatttaatacaccattttgacgttctgaaccatcaattttccacttaacgttgatgtccttagggtagaaattgttcaaaaaacatacgacagaggcaccacctgatgtcagttgctcagatgaaggagggaaaatagagactgttggtgcggcatcagctcttttgatttcaagcttggtaccggagccaaaagtaaatggaatatgtgatccctggaagcaatagtaaacgcctagatcttcagcttctacccttgatatctttagtgtgaaatccgtaccgcttccggatccactaaatctgtctggaacgccggaaaatctgtttgatactttgtagatcagaagttttggggattgaccaggcttttgcaaataccattcaaagtatgtattacctgatgaatggacaatgctctgtgatgatttacaggaaatagaagcttgatctcccaaactgactggcagagacaatggggtttgggtcataaggacgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K772100_sequence/1
type: Sequence
persistent identity: BBa K772100 sequence
display id: BBa_K772100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaagagcatgtctagcatggtgtcagacaccagttgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatgagcaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgagtgaattggtgtactcgcaataaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatatactagagctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243003_sequence/1
type: Sequence
persistent identity: BBa K243003 sequence
display id: BBa_K243003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtgtaccacgacggcgcctgccccgaagtgaagcccgtggacaacttcgactggtcccagtaccacggcaagtggtggcaggtggccgcttatcccgaccacatcaccaagtacggcaagtgcggctgggccgagtacacccccgagggcaagagcgtgaaggtgtcccggtacagcgtgatccacggcaaagagtacttcagcgagggcaccgcctaccctgtgggcgacagcaagatcggcaagatctaccacagctacaccatcggcggcgtgacccaggtgggcgtgagcaacgtgctgtccaccgacaacaagaactacatcatcggctacttttgcagatacgacgaggacaagaagggccactgggacgccgtgtgggtgctgtcccggtccatggtgctgaccggcgaggccaagaccgccgtggagaactacctgatcggcagccccgtggtggacagccagaaactggtgtacagcgacttctccgaggccgcctgcaaagtgaacaacagcaactggtcccacccccagttcgaaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K772002_sequence/1
type: Sequence
persistent identity: BBa K772002 sequence
display id: BBa_K772002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaatactagatggactacaaagacgacgacgacaaaggtggaggaggttctggaggcggtggaagtggtggcggaggtagcggagaaagcttgtttaagggaccacgtgattacaacccgatatcgagcaccatttgtcacttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactggtggaggaggttctggaggcggtggaagtggtggcggaggtagcaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcggtggaggaggttctggaggcggtggaagtggtggcggaggtagccaggttaaattacaacagagcggtgcagaactggttcgtccgggtgcaagcgttaaactgagctgtaaagcaagcggttatacctttaccaattattggattaattgggttaaacagcgtccgggtcagggtctggaatggattggtaatatttatccgagctatatttataccaattataatcaggaatttaaagataaagttaccctgaccgttgatgaaagcagcagcaccgcatatatgcagctgagcagcccgaccagcgaagatagcgcagtttattattgtacccgtagcccgtatggttatgatgaatatggtctggattattggggtcagggtaccaccgttaccgttagcagctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z