//cds/membrane/receptor
type: Collection
persistent identity: cds membrane receptor
display id: cds_membrane_receptor
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K216004
member: BBa_K389015
member: virA
member: BBa_K772002
member: YtvA
member: BBa_J07009
member: BBa_K1909010
member: BBa_K1072017
member: BBa_K1909003
member: BBa_K1909006
member: VirA/G
member: BBa_K1022100
member: BBa_K786002
member: cph8
member: BBa_J52035
member: TapZ
member: BBa_K133069
member: BBa_K259010
member: BBa_K1909009
member: BBa_I712002
member: BBa_K1660006
member: Cripto-1
member: BBa_K533008
member: BBa_K1909012
member: BBa_K209401
member: BBa_K1897002
member: BBa_K786001
member: BBa_K133063
member: lamB
member: BBa_K1909008
member: BBa_K909008
member: BBa_K209400
member: BBa_K533001
member: BBa_K772100
member: CPX
member: BBa_K1909007
member: mTaz
member: BBa_K343007
member: BBa_I712017
member: BBa_K133067
member: BBa_I712012
member: BBa_K389016
member: BBa_K1315002
member: BBa_K389421
member: BBa_K976002
member: BBa_I712003
member: BBa_K1909011
member: BBa_K533007
member: BBa_K133064
member: trz
member: BBa_K1072018
member: BBa_K133060
member: BBa_K1992004
member: BBa_K533011
member: BBa_K950006
member: EnvZ Tail
member: BBa_K389422
member: BBa_K786003
member: BBa_K133065
member: BBa_K1587008
member: VanBP
member: tar-envZ
member: BBa_K845006
member: BBa_K1992005
member: BBa_K259001
member: BBa_I712010
member: BBa_I15010
member: BBa_K533010
member: BBa_K950007
member: FGFR-1
member: BBa_K786004

BBa_K216004
type: ComponentDefinition
persistent identity: BBa K216004
display id: BBa_K216004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trz hybrid signal transduction protein
creator: Edinburgh iGEM 2009
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163. This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present.
mutable notes: No special considerations.
mutable provenance: Kindly supplied by Prof. G. Hazelbauer, and BioBricked by the University of Edinburgh iGEM team 2009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: STOP
sequence annotation: RBS
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_K216004_sequence/1

STOP
type: SequenceAnnotation
persistent identity: annotation2027069
display id: annotation2027069
version: 1
top level: BBa_K216004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216004/annotation2027069/range2027069/1

https://synbiohub.org/public/igem/BBa_K216004/annotation2027069/range2027069/1
type: Range
persistent identity: range2027069
display id: range2027069
version: 1
direction: direction/forward
top level: BBa_K216004
start: 1500
end: 1505
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2027067
display id: annotation2027067
version: 1
top level: BBa_K216004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216004/annotation2027067/range2027067/1

https://synbiohub.org/public/igem/BBa_K216004/annotation2027067/range2027067/1
type: Range
persistent identity: range2027067
display id: range2027067
version: 1
direction: direction/forward
top level: BBa_K216004
start: 4
end: 8
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2027068
display id: annotation2027068
version: 1
top level: BBa_K216004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216004/annotation2027068/range2027068/1

https://synbiohub.org/public/igem/BBa_K216004/annotation2027068/range2027068/1
type: Range
persistent identity: range2027068
display id: range2027068
version: 1
direction: direction/forward
top level: BBa_K216004
start: 18
end: 20
orientation: inline

BBa_K1909012
type: ComponentDefinition
persistent identity: BBa K1909012
display id: BBa_K1909012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Weak Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:47:22Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0082
component: BBa_K608007
sequence annotation: BBa_C0082
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1909012_sequence/1

BBa_C0082
type: Component
persistent identity: component2508813
display id: component2508813
version: 1
top level: BBa_K1909012
access: public
definition: tar-envZ

BBa_K608007
type: Component
persistent identity: component2508807
display id: component2508807
version: 1
top level: BBa_K1909012
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508813
display id: annotation2508813
version: 1
top level: BBa_K1909012
location: https://synbiohub.org/public/igem/BBa_K1909012/annotation2508813/range2508813/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909012/annotation2508813/range2508813/1
type: Range
persistent identity: range2508813
display id: range2508813
version: 1
direction: direction/reverse
top level: BBa_K1909012
start: 64
end: 1579
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2508807
display id: annotation2508807
version: 1
top level: BBa_K1909012
location: https://synbiohub.org/public/igem/BBa_K1909012/annotation2508807/range2508807/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1909012/annotation2508807/range2508807/1
type: Range
persistent identity: range2508807
display id: range2508807
version: 1
direction: direction/reverse
top level: BBa_K1909012
start: 1
end: 57
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA receptor (expressed)
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K389001
component: BBa_B0034
component: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_K389001
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
access: public
definition: virA

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
direction: direction/reverse
top level: virA
start: 44
end: 55
orientation: inline

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
direction: direction/reverse
top level: virA
start: 62
end: 2566
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
direction: direction/reverse
top level: virA
start: 1
end: 35
orientation: inline

CPX
type: ComponentDefinition
persistent identity: BBa I728500
display id: BBa_I728500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide
creator: Toan Tran-Phu
created: 2007-07-30T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _138_
m user id: 0
owner id: 1695
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be filled in later.
mutable notes: Max of XXXX amino acid long peptide.
mutable provenance: Rice JJ, Schohn A, Bessette PH, Boulware KT, and Daugherty PS. Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Sci 2006 Apr; 15(4) 825-36. doi:10.1110/ps.051897806 pmid:16600968
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Native Starting Signal
sequence annotation: SfiI Restriction Site
sequence annotation: Trypsin Cleavage Site
sequence annotation: Linker
sequence annotation: S54-F148 of OmpX
sequence annotation: BglII Cut Site
sequence annotation: SalI Restriction Site
sequence annotation: T7 Antibody Tag
sequence annotation: Polystyrene Sticky Peptide
sequence annotation: Coding sequence
sequence annotation: A1-S53 of OmpX
sequence annotation: Double stop codon (TGATAA)
sequence: https://synbiohub.org/public/igem/BBa_I728500_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation1940500
display id: annotation1940500
version: 1
top level: CPX
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940500/range1940500/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940500/range1940500/1
type: Range
persistent identity: range1940500
display id: range1940500
version: 1
direction: direction/forward
top level: CPX
start: 1
end: 3
orientation: inline

Native Starting Signal
type: SequenceAnnotation
persistent identity: annotation1940501
display id: annotation1940501
version: 1
top level: CPX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940501/range1940501/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940501/range1940501/1
type: Range
persistent identity: range1940501
display id: range1940501
version: 1
direction: direction/forward
top level: CPX
start: 1
end: 69
orientation: inline

SfiI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1940502
display id: annotation1940502
version: 1
top level: CPX
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940502/range1940502/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940502/range1940502/1
type: Range
persistent identity: range1940502
display id: range1940502
version: 1
direction: direction/forward
top level: CPX
start: 70
end: 84
orientation: inline

Trypsin Cleavage Site
type: SequenceAnnotation
persistent identity: annotation1940512
display id: annotation1940512
version: 1
top level: CPX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940512/range1940512/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940512/range1940512/1
type: Range
persistent identity: range1940512
display id: range1940512
version: 1
direction: direction/forward
top level: CPX
start: 136
end: 141
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation1940504
display id: annotation1940504
version: 1
top level: CPX
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940504/range1940504/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940504/range1940504/1
type: Range
persistent identity: range1940504
display id: range1940504
version: 1
direction: direction/forward
top level: CPX
start: 175
end: 192
orientation: inline

S54-F148 of OmpX
type: SequenceAnnotation
persistent identity: annotation1940505
display id: annotation1940505
version: 1
top level: CPX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940505/range1940505/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940505/range1940505/1
type: Range
persistent identity: range1940505
display id: range1940505
version: 1
direction: direction/forward
top level: CPX
start: 193
end: 477
orientation: inline

BglII Cut Site
type: SequenceAnnotation
persistent identity: annotation1940509
display id: annotation1940509
version: 1
top level: CPX
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940509/range1940509/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940509/range1940509/1
type: Range
persistent identity: range1940509
display id: range1940509
version: 1
direction: direction/forward
top level: CPX
start: 85
end: 90
orientation: inline

SalI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1940511
display id: annotation1940511
version: 1
top level: CPX
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940511/range1940511/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940511/range1940511/1
type: Range
persistent identity: range1940511
display id: range1940511
version: 1
direction: direction/forward
top level: CPX
start: 130
end: 135
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation1940506
display id: annotation1940506
version: 1
top level: CPX
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940506/range1940506/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940506/range1940506/1
type: Range
persistent identity: range1940506
display id: range1940506
version: 1
direction: direction/forward
top level: CPX
start: 478
end: 489
orientation: inline

T7 Antibody Tag
type: SequenceAnnotation
persistent identity: annotation1940510
display id: annotation1940510
version: 1
top level: CPX
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940510/range1940510/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940510/range1940510/1
type: Range
persistent identity: range1940510
display id: range1940510
version: 1
direction: direction/forward
top level: CPX
start: 142
end: 174
orientation: inline

Polystyrene Sticky Peptide
type: SequenceAnnotation
persistent identity: annotation1940503
display id: annotation1940503
version: 1
top level: CPX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940503/range1940503/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940503/range1940503/1
type: Range
persistent identity: range1940503
display id: range1940503
version: 1
direction: direction/forward
top level: CPX
start: 91
end: 129
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1975607
display id: annotation1975607
version: 1
top level: CPX
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1975607/range1975607/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1975607/range1975607/1
type: Range
persistent identity: range1975607
display id: range1975607
version: 1
direction: direction/forward
top level: CPX
start: 1
end: 654
orientation: inline

A1-S53 of OmpX
type: SequenceAnnotation
persistent identity: annotation1940507
display id: annotation1940507
version: 1
top level: CPX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940507/range1940507/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940507/range1940507/1
type: Range
persistent identity: range1940507
display id: range1940507
version: 1
direction: direction/forward
top level: CPX
start: 490
end: 648
orientation: inline

Double stop codon (TGATAA)
type: SequenceAnnotation
persistent identity: annotation1940508
display id: annotation1940508
version: 1
top level: CPX
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I728500/annotation1940508/range1940508/1

https://synbiohub.org/public/igem/BBa_I728500/annotation1940508/range1940508/1
type: Range
persistent identity: range1940508
display id: range1940508
version: 1
direction: direction/forward
top level: CPX
start: 649
end: 654
orientation: inline

BBa_J52035
type: ComponentDefinition
persistent identity: BBa J52035
display id: BBa_J52035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88
creator: Ota Fekonja
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 799
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable description: dnMyD88 is a dominant negative form of MyD88, which has an important role in TLR signalling pathway. dnMyD88 contains only TIR domain of MyD88.
mutable notes: /
mutable provenance: more to come...
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence: https://synbiohub.org/public/igem/BBa_J52035_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation2114917
display id: annotation2114917
version: 1
top level: BBa_J52035
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52035/annotation2114917/range2114917/1

https://synbiohub.org/public/igem/BBa_J52035/annotation2114917/range2114917/1
type: Range
persistent identity: range2114917
display id: range2114917
version: 1
direction: direction/forward
top level: BBa_J52035
start: 1
end: 420
orientation: inline

BBa_K515002
type: ComponentDefinition
persistent identity: BBa K515002
display id: BBa_K515002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PA2652
creator: Atipat Patharagulpong
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: malate chemorecptor from Pseudomonas aurenginosa
mutable notes: -
mutable provenance: genomic sequence from Pseudomonas aurenginosa strain PA01
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: PA2652
sequence annotation: insulator
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K515002_sequence/1

PA2652
type: SequenceAnnotation
persistent identity: annotation2125778
display id: annotation2125778
version: 1
top level: BBa_K515002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515002/annotation2125778/range2125778/1

https://synbiohub.org/public/igem/BBa_K515002/annotation2125778/range2125778/1
type: Range
persistent identity: range2125778
display id: range2125778
version: 1
direction: direction/forward
top level: BBa_K515002
start: 36
end: 1724
orientation: inline

insulator
type: SequenceAnnotation
persistent identity: annotation2125776
display id: annotation2125776
version: 1
top level: BBa_K515002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K515002/annotation2125776/range2125776/1

https://synbiohub.org/public/igem/BBa_K515002/annotation2125776/range2125776/1
type: Range
persistent identity: range2125776
display id: range2125776
version: 1
direction: direction/forward
top level: BBa_K515002
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2125777
display id: annotation2125777
version: 1
top level: BBa_K515002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K515002/annotation2125777/range2125777/1

https://synbiohub.org/public/igem/BBa_K515002/annotation2125777/range2125777/1
type: Range
persistent identity: range2125777
display id: range2125777
version: 1
direction: direction/forward
top level: BBa_K515002
start: 16
end: 35
orientation: inline

BBa_K1909009
type: ComponentDefinition
persistent identity: BBa K1909009
display id: BBa_K1909009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Weak Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:43:16Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K1909002
component: BBa_K608007
sequence annotation: BBa_K1909002
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1909009_sequence/1

BBa_K1909002
type: Component
persistent identity: component2508766
display id: component2508766
version: 1
top level: BBa_K1909009
access: public
definition: mTaz

BBa_K608007
type: Component
persistent identity: component2508760
display id: component2508760
version: 1
top level: BBa_K1909009
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2508766
display id: annotation2508766
version: 1
top level: BBa_K1909009
location: https://synbiohub.org/public/igem/BBa_K1909009/annotation2508766/range2508766/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909009/annotation2508766/range2508766/1
type: Range
persistent identity: range2508766
display id: range2508766
version: 1
direction: direction/reverse
top level: BBa_K1909009
start: 64
end: 1515
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2508760
display id: annotation2508760
version: 1
top level: BBa_K1909009
location: https://synbiohub.org/public/igem/BBa_K1909009/annotation2508760/range2508760/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1909009/annotation2508760/range2508760/1
type: Range
persistent identity: range2508760
display id: range2508760
version: 1
direction: direction/reverse
top level: BBa_K1909009
start: 1
end: 57
orientation: inline

BBa_K1992004
type: ComponentDefinition
persistent identity: BBa K1992004
display id: BBa_K1992004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar expression system (promoter+RBS+coding+terminator)
creator: Tzila Davidov
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T03:15:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2459_
m user id: 30463
owner id: 30463
owning group id: 9
status: status/Planning
bookmark: false
mutable description: introduction Tar chemoreceptor is one of four native chemoreceptors of E.coli, it is mediate chemotaxis response towards aspartate and away from Ni and Co (1). This device allows the expression of a functionally native Tar chemoreceptor. usage and biology This device constructed from strong promoter (J23100) and RBS (B0034) ensuring high expression of Tar chemoreceptor. High expression of a sole chemoreceptor increase the sensitivity of the bacteria to the substances that interacts with this receptor (מקור). This property makes the system efficient and useful even in low concentrations of desired materials. expriments and results In order to test the device it was cloned to chemoreceptor free strain (UU1250) and tested for chemotaxis ability using swarming assay. As can be seen in figure 1 the cells expressing the Tar chemoreceptor create a halo indicating a functional chemotaxis result, which compared to the negative and positive controls. The halo can be seen in a matter of hours ensuring that the halo is a result of a chemotaxis response. Figure 1 Fig1. (a) Tar expression in UU1250 strain, resulting a halo indicating a functional chemotaxis response. (b) Negative control- UU1250 strain w/o the Tar expression plasmid. (c) positive control - ΔZ strain expressing all chemoreceptors. The part was successfully sequenced (fig2) ensuring the present of the promoter, RBS, Tar receptor and terminator. Figure 2 Fig 2. (a) Sequencing chromatogram of the K1992004 BioBrick- the RBS is marked. (b) The alignment of the sequencing results in compared to the designed sequence - alignment was done using snapgene software.
mutable notes: none
mutable provenance: All parts were taken form the iGEM kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_K777000
component: BBa_J23100
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K1992004_sequence/1

BBa_B0015
type: Component
persistent identity: component2501562
display id: component2501562
version: 1
top level: BBa_K1992004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2501551
display id: component2501551
version: 1
top level: BBa_K1992004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K777000
type: Component
persistent identity: component2501555
display id: component2501555
version: 1
top level: BBa_K1992004
access: public
definition: https://synbiohub.org/public/igem/BBa_K777000/1

BBa_J23100
type: Component
persistent identity: component2501549
display id: component2501549
version: 1
top level: BBa_K1992004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2501562
display id: annotation2501562
version: 1
top level: BBa_K1992004
location: https://synbiohub.org/public/igem/BBa_K1992004/annotation2501562/range2501562/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1992004/annotation2501562/range2501562/1
type: Range
persistent identity: range2501562
display id: range2501562
version: 1
direction: direction/reverse
top level: BBa_K1992004
start: 1732
end: 1860
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2501549
display id: annotation2501549
version: 1
top level: BBa_K1992004
location: https://synbiohub.org/public/igem/BBa_K1992004/annotation2501549/range2501549/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1992004/annotation2501549/range2501549/1
type: Range
persistent identity: range2501549
display id: range2501549
version: 1
direction: direction/reverse
top level: BBa_K1992004
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2501551
display id: annotation2501551
version: 1
top level: BBa_K1992004
location: https://synbiohub.org/public/igem/BBa_K1992004/annotation2501551/range2501551/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1992004/annotation2501551/range2501551/1
type: Range
persistent identity: range2501551
display id: range2501551
version: 1
direction: direction/reverse
top level: BBa_K1992004
start: 44
end: 55
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2501555
display id: annotation2501555
version: 1
top level: BBa_K1992004
location: https://synbiohub.org/public/igem/BBa_K1992004/annotation2501555/range2501555/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K1992004/annotation2501555/range2501555/1
type: Range
persistent identity: range2501555
display id: range2501555
version: 1
direction: direction/reverse
top level: BBa_K1992004
start: 62
end: 1723
orientation: inline

BBa_K1072017
type: ComponentDefinition
persistent identity: BBa K1072017
display id: BBa_K1072017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072020
component: BBa_K1072013
sequence annotation: BBa_K1072020
sequence annotation: BBa_K1072013
sequence: https://synbiohub.org/public/igem/BBa_K1072017_sequence/1

BBa_K1072020
type: Component
persistent identity: component2359118
display id: component2359118
version: 1
top level: BBa_K1072017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072020/1

BBa_K1072013
type: Component
persistent identity: component2359111
display id: component2359111
version: 1
top level: BBa_K1072017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072013/1

BBa_K1072020
type: SequenceAnnotation
persistent identity: annotation2359118
display id: annotation2359118
version: 1
top level: BBa_K1072017
location: https://synbiohub.org/public/igem/BBa_K1072017/annotation2359118/range2359118/1
component: BBa_K1072020

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type: Range
persistent identity: range2359118
display id: range2359118
version: 1
direction: direction/reverse
top level: BBa_K1072017
start: 2700
end: 4169
orientation: inline

BBa_K1072013
type: SequenceAnnotation
persistent identity: annotation2359111
display id: annotation2359111
version: 1
top level: BBa_K1072017
location: https://synbiohub.org/public/igem/BBa_K1072017/annotation2359111/range2359111/1
component: BBa_K1072013

https://synbiohub.org/public/igem/BBa_K1072017/annotation2359111/range2359111/1
type: Range
persistent identity: range2359111
display id: range2359111
version: 1
direction: direction/reverse
top level: BBa_K1072017
start: 1
end: 2691
orientation: inline

BBa_K533007
type: ComponentDefinition
persistent identity: BBa K533007
display id: BBa_K533007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tar is a transmembrane receptor in E. coli which mediates chemotaxis towards aspartate.
mutable notes: The promoter of tar is replaced by lacI promoter and the RBS (Shine-Dalgarno sequence) is removed to reduce the expression level. As it turned out, lower level of expression ensured better chemotaxis.
mutable provenance: The sequence is cloned from the plasmid pPD12, a kind gift from Dr. Goulian.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Tar Receptor
sequence: https://synbiohub.org/public/igem/BBa_K533007_sequence/1

Tar Receptor
type: SequenceAnnotation
persistent identity: annotation2151977
display id: annotation2151977
version: 1
top level: BBa_K533007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533007/annotation2151977/range2151977/1

https://synbiohub.org/public/igem/BBa_K533007/annotation2151977/range2151977/1
type: Range
persistent identity: range2151977
display id: range2151977
version: 1
direction: direction/forward
top level: BBa_K533007
start: 1
end: 1662
orientation: inline

BBa_K950007
type: ComponentDefinition
persistent identity: BBa K950007
display id: BBa_K950007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: membrane progesterone receptor (Xenopus laevis)
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Membrane progesterone receptor of Xenopus laevis. Upon ligand binding, the receptor represses expression of Fet3 in yeast.
mutable notes: restriction sites removed and codon usage optimized for yeast
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/AAN78113.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: mPR Xenopus laevis
sequence: https://synbiohub.org/public/igem/BBa_K950007_sequence/1

mPR Xenopus laevis
type: SequenceAnnotation
persistent identity: annotation2179276
display id: annotation2179276
version: 1
top level: BBa_K950007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950007/annotation2179276/range2179276/1

https://synbiohub.org/public/igem/BBa_K950007/annotation2179276/range2179276/1
type: Range
persistent identity: range2179276
display id: range2179276
version: 1
direction: direction/forward
top level: BBa_K950007
start: 1
end: 1062
orientation: inline

BBa_K133069
type: ComponentDefinition
persistent identity: BBa K133069
display id: BBa_K133069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TMTIR)TLR3stop
creator: Katja Kolar
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Toll-Like Receptor 3 transmembrane region and cytoplasmic domain, with stop codon added.
mutable provenance: amplified from TLR3 gene, obtained from Invivogen, plasmid pUNO-hTLR3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133069_sequence/1

BBa_K389422
type: ComponentDefinition
persistent identity: BBa K389422
display id: BBa_K389422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389402
sequence annotation: BBa_K389402
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389422_sequence/1

BBa_K389017
type: Component
persistent identity: component2313896
display id: component2313896
version: 1
top level: BBa_K389422
access: public
definition: VirA/G

BBa_K389402
type: Component
persistent identity: component2313920
display id: component2313920
version: 1
top level: BBa_K389422
access: public
definition: https://synbiohub.org/public/igem/BBa_K389402/1

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2313920
display id: annotation2313920
version: 1
top level: BBa_K389422
location: https://synbiohub.org/public/igem/BBa_K389422/annotation2313920/range2313920/1
component: BBa_K389402

https://synbiohub.org/public/igem/BBa_K389422/annotation2313920/range2313920/1
type: Range
persistent identity: range2313920
display id: range2313920
version: 1
direction: direction/reverse
top level: BBa_K389422
start: 3708
end: 6594
orientation: inline

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313896
display id: annotation2313896
version: 1
top level: BBa_K389422
location: https://synbiohub.org/public/igem/BBa_K389422/annotation2313896/range2313896/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389422/annotation2313896/range2313896/1
type: Range
persistent identity: range2313896
display id: range2313896
version: 1
direction: direction/reverse
top level: BBa_K389422
start: 1
end: 3699
orientation: inline

BBa_K1909007
type: ComponentDefinition
persistent identity: BBa K1909007
display id: BBa_K1909007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:41:12Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 0
m user id: 29525
owner id: 29525
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K1909002
component: BBa_K081005
sequence annotation: BBa_K1909002
sequence annotation: BBa_K081005
sequence: https://synbiohub.org/public/igem/BBa_K1909007_sequence/1

BBa_K1909002
type: Component
persistent identity: component2507551
display id: component2507551
version: 1
top level: BBa_K1909007
access: public
definition: mTaz

BBa_K081005
type: Component
persistent identity: component2507545
display id: component2507545
version: 1
top level: BBa_K1909007
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2507551
display id: annotation2507551
version: 1
top level: BBa_K1909007
location: https://synbiohub.org/public/igem/BBa_K1909007/annotation2507551/range2507551/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909007/annotation2507551/range2507551/1
type: Range
persistent identity: range2507551
display id: range2507551
version: 1
direction: direction/reverse
top level: BBa_K1909007
start: 65
end: 1516
orientation: inline

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2507545
display id: annotation2507545
version: 1
top level: BBa_K1909007
location: https://synbiohub.org/public/igem/BBa_K1909007/annotation2507545/range2507545/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K1909007/annotation2507545/range2507545/1
type: Range
persistent identity: range2507545
display id: range2507545
version: 1
direction: direction/reverse
top level: BBa_K1909007
start: 1
end: 58
orientation: inline

BBa_K1897002
type: ComponentDefinition
persistent identity: BBa K1897002
display id: BBa_K1897002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasR Membrane receptor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T02:50:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
mutable notes: NIL
mutable provenance: The HasR gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HasR
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1897002_sequence/1

HasR
type: SequenceAnnotation
persistent identity: annotation2489470
display id: annotation2489470
version: 1
top level: BBa_K1897002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1
type: Range
persistent identity: range2489470
display id: range2489470
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 2700
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2489469
display id: annotation2489469
version: 1
top level: BBa_K1897002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1
type: Range
persistent identity: range2489469
display id: range2489469
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2489471
display id: annotation2489471
version: 1
top level: BBa_K1897002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1
type: Range
persistent identity: range2489471
display id: range2489471
version: 1
direction: direction/reverse
top level: BBa_K1897002
start: 2698
end: 2700
orientation: inline

tar-envZ
type: ComponentDefinition
persistent identity: BBa C0082
display id: BBa_C0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Receptor, tar-envZ
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes. This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
mutable notes: A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Envz (back)
sequence annotation: Tar (front)
sequence annotation: LVA
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0082_sequence/1

Envz (back)
type: SequenceAnnotation
persistent identity: annotation308360
display id: annotation308360
version: 1
top level: tar-envZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1

https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
type: Range
persistent identity: range308360
display id: range308360
version: 1
direction: direction/forward
top level: tar-envZ
start: 772
end: 1452
orientation: inline

Tar (front)
type: SequenceAnnotation
persistent identity: annotation308352
display id: annotation308352
version: 1
top level: tar-envZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1

https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
type: Range
persistent identity: range308352
display id: range308352
version: 1
direction: direction/forward
top level: tar-envZ
start: 1
end: 765
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation301146
display id: annotation301146
version: 1
top level: tar-envZ
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1

https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
type: Range
persistent identity: range301146
display id: range301146
version: 1
direction: direction/forward
top level: tar-envZ
start: 1453
end: 1485
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation300943
display id: annotation300943
version: 1
top level: tar-envZ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1

https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
type: Range
persistent identity: range300943
display id: range300943
version: 1
direction: direction/forward
top level: tar-envZ
start: 423
end: 424
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213997
display id: annotation2213997
version: 1
top level: tar-envZ
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1

https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
type: Range
persistent identity: range2213997
display id: range2213997
version: 1
direction: direction/forward
top level: tar-envZ
start: 1492
end: 1516
orientation: inline

BBa_K1022100
type: ComponentDefinition
persistent identity: BBa K1022100
display id: BBa_K1022100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD:AIP receiver:GFP:TT
creator: Maithili Krishnan
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:08:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This plasmid contains code for pBAD promoter, AIP receiver Agr A and Agr C, GFP along with double terminator.
mutable notes: Sequence was cloned using BBa_E0240 and BBa_I746103 from iGEM parts registry.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0240
component: BBa_I746103
component: BBa_K206000
sequence annotation: BBa_I746103
sequence annotation: BBa_K206000
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1022100_sequence/1

BBa_E0240
type: Component
persistent identity: component2332910
display id: component2332910
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_I746103
type: Component
persistent identity: component2332899
display id: component2332899
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_I746103/1

BBa_K206000
type: Component
persistent identity: component2332885
display id: component2332885
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_I746103
type: SequenceAnnotation
persistent identity: annotation2332899
display id: annotation2332899
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
component: BBa_I746103

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
type: Range
persistent identity: range2332899
display id: range2332899
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 139
end: 2299
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2332885
display id: annotation2332885
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
type: Range
persistent identity: range2332885
display id: range2332885
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 1
end: 130
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2332910
display id: annotation2332910
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
type: Range
persistent identity: range2332910
display id: range2332910
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 2308
end: 3183
orientation: inline

fhuA
type: ComponentDefinition
persistent identity: BBa K259000
display id: BBa_K259000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fhuA - Outer membrane transporter for ferrichrome-iron
creator: Petros Mina
created: 2009-07-13T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
experience: experience/None
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: fhuA is an outer membrane (OM) protein that is found in the gram negative bacterium, E.coli. It is involved in the active-transport (energy-dependent uptake) of ferrichrome-iron from the extracellular environment. However fhuA is only able to bind ferrichrome-iron (i.e. ferrichrome iron is a ligand of fhuA protein). For the uptake to be completed fhuA needs to form a complex with another protein, tonB that carries out the energy-dependent uptake by using ATP as substrate to undergo the relevant conformational changes. This biobrick ONLY encodes for fhuA. fhuA is a beta-barell protein (22 anti-parallel beta-strands) and is composed of 747 aminoacids. This receptor protein may be used as an attachment point by some colicins. References: Locher K.P., Rees B., Koebnik R., Mitschler A., Moulinier L., Rosenusch J.P. and Moras D. (1998),Transmembrane signalling across the Ligand-Gated FhuAReceptor: Crystal structures of Free and Ferrichrome-Bound States reveal allosteric changes. Cell 95,771-778. Uniprot access number: P06971 Ecogene: http://ecogene.org/geneInfo.php?eg_id=EG10302
mutable notes: The coding sequence (CDS) was already in compliance with assembly standard 10 (RFC10).
mutable provenance: fhuA is part of the chromosomal genome of E.coli. In particular it can be found at 3.61min and follows the clockwise direction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Multipass (OM-Perpiplasm) protein
sequence annotation: Signal Sequence
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K259000_sequence/1

Multipass (OM-Perpiplasm) protein
type: SequenceAnnotation
persistent identity: annotation2011311
display id: annotation2011311
version: 1
top level: fhuA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011311/range2011311/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011311/range2011311/1
type: Range
persistent identity: range2011311
display id: range2011311
version: 1
direction: direction/forward
top level: fhuA
start: 100
end: 2241
orientation: inline

Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2011310
display id: annotation2011310
version: 1
top level: fhuA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011310/range2011310/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011310/range2011310/1
type: Range
persistent identity: range2011310
display id: range2011310
version: 1
direction: direction/forward
top level: fhuA
start: 1
end: 99
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2011290
display id: annotation2011290
version: 1
top level: fhuA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011290/range2011290/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011290/range2011290/1
type: Range
persistent identity: range2011290
display id: range2011290
version: 1
direction: direction/forward
top level: fhuA
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2011291
display id: annotation2011291
version: 1
top level: fhuA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259000/annotation2011291/range2011291/1

https://synbiohub.org/public/igem/BBa_K259000/annotation2011291/range2011291/1
type: Range
persistent identity: range2011291
display id: range2011291
version: 1
direction: direction/forward
top level: fhuA
start: 2242
end: 2247
orientation: inline

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS 1.0
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: HindIII for N-terminal fusin
sequence annotation: SRII-HtrII-Tar
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

EnvZ Tail
type: ComponentDefinition
persistent identity: BBa K1909005
display id: BBa_K1909005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Transduction and Kinase Domains
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: E. coli EnvZ (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence: https://synbiohub.org/public/igem/BBa_K1909005_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507346
display id: annotation2507346
version: 1
top level: EnvZ Tail
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1

https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1
type: Range
persistent identity: range2507346
display id: range2507346
version: 1
direction: direction/forward
top level: EnvZ Tail
start: 1
end: 882
orientation: inline

BBa_K1315002
type: ComponentDefinition
persistent identity: BBa K1315002
display id: BBa_K1315002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfC gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the coding sequence for the RpfC(regulation of pathogenicity factor C) protein. It is the sensor in the two-component signal transduction of Xcc that activates the regulator RpfG(Bba_K1315003) in the presence of DSF.
mutable notes: The sequence had 1 EcoRI site and 3 PstI sites that were all mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is ''Xanthomonas campestris pv. campestris''(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfC
sequence: https://synbiohub.org/public/igem/BBa_K1315002_sequence/1

RpfC
type: SequenceAnnotation
persistent identity: annotation2386328
display id: annotation2386328
version: 1
top level: BBa_K1315002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1

https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1
type: Range
persistent identity: range2386328
display id: range2386328
version: 1
direction: direction/forward
top level: BBa_K1315002
start: 1
end: 2181
orientation: inline

BBa_K1909006
type: ComponentDefinition
persistent identity: BBa K1909006
display id: BBa_K1909006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ-LRR chimeric receptor for mDAP recognition
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:35:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 0
m user id: 29525
owner id: 29525
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K1909003
component: BBa_K1909005
component: BBa_K1909004
sequence annotation: BBa_K1909005
sequence annotation: BBa_K1909003
sequence annotation: BBa_K1909004
sequence: https://synbiohub.org/public/igem/BBa_K1909006_sequence/1

BBa_K1909003
type: Component
persistent identity: component2507398
display id: component2507398
version: 1
top level: BBa_K1909006
access: public
definition: BBa_K1909003

BBa_K1909005
type: Component
persistent identity: component2507407
display id: component2507407
version: 1
top level: BBa_K1909006
access: public
definition: EnvZ Tail

BBa_K1909004
type: Component
persistent identity: component2507405
display id: component2507405
version: 1
top level: BBa_K1909006
access: public
definition: BBa_K1909004

BBa_K1909005
type: SequenceAnnotation
persistent identity: annotation2507407
display id: annotation2507407
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
component: BBa_K1909005

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
type: Range
persistent identity: range2507407
display id: range2507407
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1143
end: 2024
orientation: inline

BBa_K1909003
type: SequenceAnnotation
persistent identity: annotation2507398
display id: annotation2507398
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
component: BBa_K1909003

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
type: Range
persistent identity: range2507398
display id: range2507398
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1
end: 161
orientation: inline

BBa_K1909004
type: SequenceAnnotation
persistent identity: annotation2507405
display id: annotation2507405
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
component: BBa_K1909004

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
type: Range
persistent identity: range2507405
display id: range2507405
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 168
end: 1136
orientation: inline

Cripto-1
type: ComponentDefinition
persistent identity: BBa K1908001
display id: BBa_K1908001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for Homo sapien Cripto-1 membrane protein.
creator: Ryan Kawalerski, Tasnia Islam, Joshua Hombrebueno, Sarah Heacox, Samara Khan, Daniel Thach, Meghan Bialt deCelie.
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T10:51:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: S. Cerevisiae Kozak Sequence
sequence annotation: stop
sequence annotation: ADH1 Terminator
sequence annotation: BBa_K1908001
sequence: https://synbiohub.org/public/igem/BBa_K1908001_sequence/1

S. Cerevisiae Kozak Sequence
type: SequenceAnnotation
persistent identity: annotation2520173
display id: annotation2520173
version: 1
top level: Cripto-1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520173/range2520173/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520173/range2520173/1
type: Range
persistent identity: range2520173
display id: range2520173
version: 1
direction: direction/forward
top level: Cripto-1
start: 1
end: 9
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2520174
display id: annotation2520174
version: 1
top level: Cripto-1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520174/range2520174/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520174/range2520174/1
type: Range
persistent identity: range2520174
display id: range2520174
version: 1
direction: direction/forward
top level: Cripto-1
start: 574
end: 579
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2520178
display id: annotation2520178
version: 1
top level: Cripto-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2520178/range2520178/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2520178/range2520178/1
type: Range
persistent identity: range2520178
display id: range2520178
version: 1
direction: direction/forward
top level: Cripto-1
start: 580
end: 767
orientation: inline

BBa_K1908001
type: SequenceAnnotation
persistent identity: annotation2511513
display id: annotation2511513
version: 1
top level: Cripto-1
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1908001/annotation2511513/range2511513/1

https://synbiohub.org/public/igem/BBa_K1908001/annotation2511513/range2511513/1
type: Range
persistent identity: range2511513
display id: range2511513
version: 1
direction: direction/forward
top level: Cripto-1
start: 10
end: 573
orientation: inline

BBa_K389423
type: ComponentDefinition
persistent identity: BBa K389423
display id: BBa_K389423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389403
sequence annotation: BBa_K389017
sequence annotation: BBa_K389403
sequence: https://synbiohub.org/public/igem/BBa_K389423_sequence/1

BBa_K389017
type: Component
persistent identity: component2313944
display id: component2313944
version: 1
top level: BBa_K389423
access: public
definition: VirA/G

BBa_K389403
type: Component
persistent identity: component2313969
display id: component2313969
version: 1
top level: BBa_K389423
access: public
definition: https://synbiohub.org/public/igem/BBa_K389403/1

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313944
display id: annotation2313944
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313944/range2313944/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389423/annotation2313944/range2313944/1
type: Range
persistent identity: range2313944
display id: range2313944
version: 1
direction: direction/reverse
top level: BBa_K389423
start: 1
end: 3699
orientation: inline

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2313969
display id: annotation2313969
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313969/range2313969/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389423/annotation2313969/range2313969/1
type: Range
persistent identity: range2313969
display id: range2313969
version: 1
direction: direction/reverse
top level: BBa_K389423
start: 3708
end: 6621
orientation: inline

YtvA
type: ComponentDefinition
persistent identity: BBa K143037
display id: BBa_K143037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YtvA Blue Light Receptor for B.subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: YtvA is a blue light receptor endogenously found in ''B.subtilis''. Activation of the YtvA light receptor activates the mild stress response pathway, accumulating in sigma B activation. Previous research has shown that sigma B dependent transcription in response to blue light light exposure can be enhanced by the over expression of the YtvA receptor 1. We over expressed YtvA to enhance the transcriptional output of sigma B promoters in response to blue light.
mutable notes: Biobrick standard was applied to the YtvA sequence.
mutable provenance: The part was designed using the sequence from the B.subtilis genome. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YtvA Blue Light Receptor for B.subtilis
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143037_sequence/1

YtvA Blue Light Receptor for B.subtilis
type: SequenceAnnotation
persistent identity: annotation1976412
display id: annotation1976412
version: 1
top level: YtvA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1976412/range1976412/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1976412/range1976412/1
type: Range
persistent identity: range1976412
display id: range1976412
version: 1
direction: direction/forward
top level: YtvA
start: 1
end: 783
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992714
display id: annotation1992714
version: 1
top level: YtvA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992714/range1992714/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992714/range1992714/1
type: Range
persistent identity: range1992714
display id: range1992714
version: 1
direction: direction/forward
top level: YtvA
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992715
display id: annotation1992715
version: 1
top level: YtvA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992715/range1992715/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992715/range1992715/1
type: Range
persistent identity: range1992715
display id: range1992715
version: 1
direction: direction/forward
top level: YtvA
start: 787
end: 789
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992716
display id: annotation1992716
version: 1
top level: YtvA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992716/range1992716/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992716/range1992716/1
type: Range
persistent identity: range1992716
display id: range1992716
version: 1
direction: direction/forward
top level: YtvA
start: 784
end: 786
orientation: inline

BBa_K389015
type: ComponentDefinition
persistent identity: BBa K389015
display id: BBa_K389015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G reporter device luc
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is for testing the virA/G signaling system. The read-out is the luciferase <partinfo>K389004</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
mutable notes: this is only for measuring the natural virA/G system
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene, Promega's pGL4.10luc2 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389012
component: BBa_K389010
sequence annotation: BBa_K389010
sequence annotation: BBa_K389012
sequence: https://synbiohub.org/public/igem/BBa_K389015_sequence/1

BBa_K389012
type: Component
persistent identity: component2227573
display id: component2227573
version: 1
top level: BBa_K389015
access: public
definition: https://synbiohub.org/public/igem/BBa_K389012/1

BBa_K389010
type: Component
persistent identity: component2227555
display id: component2227555
version: 1
top level: BBa_K389015
access: public
definition: virA

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2227555
display id: annotation2227555
version: 1
top level: BBa_K389015
location: https://synbiohub.org/public/igem/BBa_K389015/annotation2227555/range2227555/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389015/annotation2227555/range2227555/1
type: Range
persistent identity: range2227555
display id: range2227555
version: 1
direction: direction/reverse
top level: BBa_K389015
start: 1
end: 2566
orientation: inline

BBa_K389012
type: SequenceAnnotation
persistent identity: annotation2227573
display id: annotation2227573
version: 1
top level: BBa_K389015
location: https://synbiohub.org/public/igem/BBa_K389015/annotation2227573/range2227573/1
component: BBa_K389012

https://synbiohub.org/public/igem/BBa_K389015/annotation2227573/range2227573/1
type: Range
persistent identity: range2227573
display id: range2227573
version: 1
direction: direction/reverse
top level: BBa_K389015
start: 2575
end: 5361
orientation: inline

BBa_K133063
type: ComponentDefinition
persistent identity: BBa K133063
display id: BBa_K133063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TIR)TLR3
creator: Katja Kolar
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133063_sequence/1

BBa_K209400
type: ComponentDefinition
persistent identity: BBa K209400
display id: BBa_K209400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI B-C part, hM4D
creator: Benjamin Rhau
created: 2009-09-28T11:00:00Z
modified: 2015-05-08T01:11:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _312_
m user id: 0
owner id: 3698
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: hM4 DREADD flanked by AarI BC sites.
mutable notes: Knocked out AarI recognition site.
mutable provenance: plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K209400_sequence/1

BBa_K772002
type: ComponentDefinition
persistent identity: BBa K772002
display id: BBa_K772002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: N terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: CD215 antibody
sequence annotation: TEV Protease N terminus
sequence annotation: OmpA (Dock)
sequence annotation: Binding Dock
sequence annotation: pCons Promoter
sequence: https://synbiohub.org/public/igem/BBa_K772002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203683
display id: annotation2203683
version: 1
top level: BBa_K772002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1
type: Range
persistent identity: range2203683
display id: range2203683
version: 1
direction: direction/forward
top level: BBa_K772002
start: 73
end: 93
orientation: inline

CD215 antibody
type: SequenceAnnotation
persistent identity: annotation2203686
display id: annotation2203686
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1
type: Range
persistent identity: range2203686
display id: range2203686
version: 1
direction: direction/forward
top level: BBa_K772002
start: 837
end: 1138
orientation: inline

TEV Protease N terminus
type: SequenceAnnotation
persistent identity: annotation2203685
display id: annotation2203685
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1
type: Range
persistent identity: range2203685
display id: range2203685
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 398
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203682
display id: annotation2203682
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1
type: Range
persistent identity: range2203682
display id: range2203682
version: 1
direction: direction/forward
top level: BBa_K772002
start: 399
end: 836
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203684
display id: annotation2203684
version: 1
top level: BBa_K772002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1
type: Range
persistent identity: range2203684
display id: range2203684
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 1138
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203681
display id: annotation2203681
version: 1
top level: BBa_K772002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1
type: Range
persistent identity: range2203681
display id: range2203681
version: 1
direction: direction/forward
top level: BBa_K772002
start: 1
end: 72
orientation: inline

BBa_K133065
type: ComponentDefinition
persistent identity: BBa K133065
display id: BBa_K133065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TMTIR)TLR3
creator: Katja Kolar
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 3358
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133065_sequence/1

BBa_K772001
type: ComponentDefinition
persistent identity: BBa K772001
display id: BBa_K772001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: C-terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: OmpA (Dock)
sequence annotation: RBS
sequence annotation: Binding Dock
sequence annotation: pCons Promoter
sequence annotation: CD215 Antibody
sequence annotation: TEV Protease C terminus
sequence: https://synbiohub.org/public/igem/BBa_K772001_sequence/1

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203671
display id: annotation2203671
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1
type: Range
persistent identity: range2203671
display id: range2203671
version: 1
direction: direction/forward
top level: BBa_K772001
start: 420
end: 857
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203670
display id: annotation2203670
version: 1
top level: BBa_K772001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1
type: Range
persistent identity: range2203670
display id: range2203670
version: 1
direction: direction/forward
top level: BBa_K772001
start: 73
end: 93
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203674
display id: annotation2203674
version: 1
top level: BBa_K772001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1
type: Range
persistent identity: range2203674
display id: range2203674
version: 1
direction: direction/forward
top level: BBa_K772001
start: 94
end: 1159
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203669
display id: annotation2203669
version: 1
top level: BBa_K772001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1
type: Range
persistent identity: range2203669
display id: range2203669
version: 1
direction: direction/forward
top level: BBa_K772001
start: 1
end: 72
orientation: inline

CD215 Antibody
type: SequenceAnnotation
persistent identity: annotation2203673
display id: annotation2203673
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1
type: Range
persistent identity: range2203673
display id: range2203673
version: 1
direction: direction/forward
top level: BBa_K772001
start: 858
end: 1159
orientation: inline

TEV Protease C terminus
type: SequenceAnnotation
persistent identity: annotation2203672
display id: annotation2203672
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1

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persistent identity: range2203672
display id: range2203672
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start: 94
end: 419
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BBa_K343007
type: ComponentDefinition
persistent identity: BBa K343007
display id: BBa_K343007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Photosensor generator
creator: Maria Mollerup
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _465_
m user id: 0
owner id: 6250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Molecular mechanism of the photosensor The fusion,chimera-protein coupled to the chemotaxis pathway. Figure taken from Trivedi et al.(2) The fusion,chimera-protein coupled to the chemotaxis pathway. Figure taken from Trivedi et al.(2) The photosensor acts directly on the tumbling frequency by effecting E. Coli's normal chemotaxis pathway. When exposed to bluelight, the sensory rhodopsin II will absorb the photons and undergo a change in ultrastructure, which is being transduced through HtrII on to the Tar domain. This effects decreases the autophosphorylation of the CheA protein, which in turn again decreases the amount of phosphorylated CheY, which just means that less of it will get phosphorylated. If there is less of the CheY-P, then there is a smaller chance of one of these molecules binding to the flagellar motor and making it turn clockwise, thereby inducing a lowered tumbling frequency in the system. The photosensor can also act in the opposite way, inducing a higher tumbling rate in the bacteria. Which effect the sensor will have depends on where NpHtrII and StTar are fused in the HAMP domain. If the fusion contains 20 more basepair of the HtrII domain and 20 less of the Tar domain, the photosensor would have the opposite effect and would be increasing the autophosphorylation of CheA. Usage and parameters The part requires retinal to work in E.Coli. This can be achieved through adding retinal to the liquid growth medium and/or the plates. Currently we are doing experiments on wether the part also functions with an internal retinal source, ie retinal synthesis in E. Coli. On top of adding retinal the cells will have to be grown in the dark for at least two hours after the addition of retinal to the growth medium if you want to see an effect. This is so that the photosensor is not exposed to light before the experiments and will result in maximum output if exposed to blue light. Results Bacteria containing this part will exhibit a lowered tumbling rate when ex…
mutable notes: Design Notes NpSopII-NpHtrII-StTar fusion-chimera protein sequence from Jung K-H, Spudich EN, Trivedi VD and Spudich JL (1). The first 224 amino acid residues come from the NpSopII gene, encoding a bluelight photon receptor with 15 residues removed at the C-terminal end. The following 9 amino acids are a linker. the last part is HtrII fused with Tsr from E.Coli at the HAMP domain. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr. The constitutive promoter R0040 was chosen for its medium strength, so that there would be a rather large amount of the fusion, chimera protein expressed, but not so much that it would be lethal to the cell. The easy repression and inhibition of repression through TetR and tetracycline makes for an easily controlled expression if that is needed. The doible terminator B0015 was chosen for it's reliability and good reviews on the partsregistry.
mutable provenance: Source Sensory Rhodopsin II and HtrII domains were taken from the genome of Natronomonas Pharaonis. The Tar methyl accepting chemotaxis protein was taken from Salmonella Enterica Serovar Typhimurium. The synthetic linker and fusing of the HAMP domains of HtrII and StTar was done by the team behind the referenced article (1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K343003
component: BBa_J13002
sequence annotation: BBa_K343003
sequence annotation: BBa_J13002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K343007_sequence/1

BBa_B0015
type: Component
persistent identity: component2103033
display id: component2103033
version: 1
top level: BBa_K343007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K343003
type: Component
persistent identity: component2103026
display id: component2103026
version: 1
top level: BBa_K343007
access: public
definition: https://synbiohub.org/public/igem/BBa_K343003/1

BBa_J13002
type: Component
persistent identity: component2103019
display id: component2103019
version: 1
top level: BBa_K343007
access: public
definition: https://synbiohub.org/public/igem/BBa_J13002/1

BBa_K343003
type: SequenceAnnotation
persistent identity: annotation2103026
display id: annotation2103026
version: 1
top level: BBa_K343007
location: https://synbiohub.org/public/igem/BBa_K343007/annotation2103026/range2103026/1
component: BBa_K343003

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persistent identity: range2103026
display id: range2103026
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start: 81
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BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2103019
display id: annotation2103019
version: 1
top level: BBa_K343007
location: https://synbiohub.org/public/igem/BBa_K343007/annotation2103019/range2103019/1
component: BBa_J13002

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type: Range
persistent identity: range2103019
display id: range2103019
version: 1
direction: direction/reverse
top level: BBa_K343007
start: 1
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2103033
display id: annotation2103033
version: 1
top level: BBa_K343007
location: https://synbiohub.org/public/igem/BBa_K343007/annotation2103033/range2103033/1
component: BBa_B0015

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type: Range
persistent identity: range2103033
display id: range2103033
version: 1
direction: direction/reverse
top level: BBa_K343007
start: 2054
end: 2182
orientation: inline

BBa_K1909003
type: ComponentDefinition
persistent identity: BBa K1909003
display id: BBa_K1909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Peptide and first Transmembrane Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:06:33Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: For EnvZ fusion protein design. Long description will be added soon.
mutable notes: coming soon
mutable provenance: Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1909003_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507125
display id: annotation2507125
version: 1
top level: BBa_K1909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1

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type: Range
persistent identity: range2507125
display id: range2507125
version: 1
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top level: BBa_K1909003
start: 1
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orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2507149
display id: annotation2507149
version: 1
top level: BBa_K1909003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1

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type: Range
persistent identity: range2507149
display id: range2507149
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 46
end: 69
orientation: inline

BBa_K786004
type: ComponentDefinition
persistent identity: BBa K786004
display id: BBa_K786004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light triggering phototaxis using chp8
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Red light triggering phototaxis using chp8, which can sense the red light from 600-700 nm.
mutable notes: 6 poly-His tag was positioned at C-terminal of the protein.
mutable provenance: The sequence of chp8 is from previous biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: poly-HIS tag
sequence annotation: chp8 protein
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786004_sequence/1

poly-HIS tag
type: SequenceAnnotation
persistent identity: annotation2190190
display id: annotation2190190
version: 1
top level: BBa_K786004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1

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type: Range
persistent identity: range2190190
display id: range2190190
version: 1
direction: direction/forward
top level: BBa_K786004
start: 2301
end: 2321
orientation: inline

chp8 protein
type: SequenceAnnotation
persistent identity: annotation2190189
display id: annotation2190189
version: 1
top level: BBa_K786004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1

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type: Range
persistent identity: range2190189
display id: range2190189
version: 1
direction: direction/forward
top level: BBa_K786004
start: 69
end: 2321
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190187
display id: annotation2190187
version: 1
top level: BBa_K786004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1

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type: Range
persistent identity: range2190187
display id: range2190187
version: 1
direction: direction/forward
top level: BBa_K786004
start: 1
end: 50
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190188
display id: annotation2190188
version: 1
top level: BBa_K786004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1

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type: Range
persistent identity: range2190188
display id: range2190188
version: 1
direction: direction/forward
top level: BBa_K786004
start: 51
end: 62
orientation: inline

FGFR-1
type: ComponentDefinition
persistent identity: BBa K976000
display id: BBa_K976000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fibroblast Growth Factor-1 Receptor Coding Sequence
creator: Amna Sohail
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Fibroblast Growth Factor-1 Receptor (FGFR-1) is a receptor that is commonly found in melanocytes and is involved with melanocyte differentiation, migration, and division. Malignant melanoma is characterized by abnormally high ligand expression of Fibroblast Growth Factor-2 (FGF2), otherwise known as the Basic Fibroblast Growth Factor (BFGF). The FGFR-1 receptor as a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor.
mutable notes: The start codon sequence has been added to the FGFR-1 coding sequence.
mutable provenance: The part has been derived from a genomic sequence provisioned by the Ensembl Database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Ig-like C2-type 3 Domain
sequence annotation: Ig-like C2-type 2 Domain
sequence annotation: Helical Transmembrane Domain
sequence annotation: Protein Kinase Domain
sequence annotation: Ig-like C2-type 1 Domain
sequence annotation: Cytoplasmic Domain
sequence annotation: Topological Extracellular Domain
sequence: https://synbiohub.org/public/igem/BBa_K976000_sequence/1

Ig-like C2-type 3 Domain
type: SequenceAnnotation
persistent identity: annotation2329993
display id: annotation2329993
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329993/range2329993/1

https://synbiohub.org/public/igem/BBa_K976000/annotation2329993/range2329993/1
type: Range
persistent identity: range2329993
display id: range2329993
version: 1
direction: direction/forward
top level: FGFR-1
start: 765
end: 1071
orientation: inline

Ig-like C2-type 2 Domain
type: SequenceAnnotation
persistent identity: annotation2329947
display id: annotation2329947
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329947/range2329947/1

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type: Range
persistent identity: range2329947
display id: range2329947
version: 1
direction: direction/forward
top level: FGFR-1
start: 474
end: 738
orientation: inline

Helical Transmembrane Domain
type: SequenceAnnotation
persistent identity: annotation2329944
display id: annotation2329944
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329944/range2329944/1

https://synbiohub.org/public/igem/BBa_K976000/annotation2329944/range2329944/1
type: Range
persistent identity: range2329944
display id: range2329944
version: 1
direction: direction/forward
top level: FGFR-1
start: 1131
end: 1191
orientation: inline

Protein Kinase Domain
type: SequenceAnnotation
persistent identity: annotation2329994
display id: annotation2329994
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329994/range2329994/1

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type: Range
persistent identity: range2329994
display id: range2329994
version: 1
direction: direction/forward
top level: FGFR-1
start: 1434
end: 2301
orientation: inline

Ig-like C2-type 1 Domain
type: SequenceAnnotation
persistent identity: annotation2329946
display id: annotation2329946
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329946/range2329946/1

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type: Range
persistent identity: range2329946
display id: range2329946
version: 1
direction: direction/forward
top level: FGFR-1
start: 75
end: 357
orientation: inline

Cytoplasmic Domain
type: SequenceAnnotation
persistent identity: annotation2329945
display id: annotation2329945
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329945/range2329945/1

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type: Range
persistent identity: range2329945
display id: range2329945
version: 1
direction: direction/forward
top level: FGFR-1
start: 1194
end: 2466
orientation: inline

Topological Extracellular Domain
type: SequenceAnnotation
persistent identity: annotation2329943
display id: annotation2329943
version: 1
top level: FGFR-1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329943/range2329943/1

https://synbiohub.org/public/igem/BBa_K976000/annotation2329943/range2329943/1
type: Range
persistent identity: range2329943
display id: range2329943
version: 1
direction: direction/forward
top level: FGFR-1
start: 66
end: 1128
orientation: inline

BBa_K1992005
type: ComponentDefinition
persistent identity: BBa K1992005
display id: BBa_K1992005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar native RBS expression system (promoter+RBS+coding+terminator)
creator: Tzila Davidov,Shilo Ohayon
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T03:18:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2459_
m user id: 30463
owner id: 30463
owning group id: 9
status: status/Planning
bookmark: true
mutable description: introduction Tar chemoreceptor is one of four native chemoreceptors of E.coli, it is mediate chemotaxis response towards aspartate and away from Ni and Co (מקור). This device allows the expression of a functionally native Tar chemoreceptor. usage and biology This device is very similar to our K1992004 device the only difference is the RBS, here the expression system is using the native RBS of the Tar receptor as found in the E.coli genome (K1992000). expriments and results In order to test the device it was cloned to chemoreceptor free strain (UU1250) and tested for chemotaxis ability using swarming assay. As can be seen in figure 1 the cells expressing the Tar chemoreceptor create a halo indicating a functional chemotaxis result, which compared to the Tar expression system using a strong RBS(B0034). Figure 1 Fig1. (a) Tar expression in UU1250 strain cloned with K1992004 expretion system - strong RBS. (b) Tar expression in UU1250 strain cloned with K1992004 expretion system - Tar native RBS The part was successfully sequenced (fig2) ensuring the present of the promoter, RBS, Tar receptor and terminator. Figure 2 Fig 2. (a) Sequencing chromatogram of the K1992005 BioBrick- the RBS is marked. (b) The alignment of the sequencing results in compared to the designed sequence - alignment was done using snapgene software.
mutable notes: none
mutable provenance: http://ecocyc.org/ and the iGEM kit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1992000
component: BBa_B0015
component: BBa_J23100
component: BBa_K777000
sequence annotation: BBa_K1992000
sequence annotation: BBa_J23100
sequence annotation: BBa_K777000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1992005_sequence/1

BBa_K1992000
type: Component
persistent identity: component2501710
display id: component2501710
version: 1
top level: BBa_K1992005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1992000/1

BBa_B0015
type: Component
persistent identity: component2501721
display id: component2501721
version: 1
top level: BBa_K1992005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23100
type: Component
persistent identity: component2501708
display id: component2501708
version: 1
top level: BBa_K1992005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777000
type: Component
persistent identity: component2501714
display id: component2501714
version: 1
top level: BBa_K1992005
access: public
definition: https://synbiohub.org/public/igem/BBa_K777000/1

BBa_K1992000
type: SequenceAnnotation
persistent identity: annotation2501710
display id: annotation2501710
version: 1
top level: BBa_K1992005
location: https://synbiohub.org/public/igem/BBa_K1992005/annotation2501710/range2501710/1
component: BBa_K1992000

https://synbiohub.org/public/igem/BBa_K1992005/annotation2501710/range2501710/1
type: Range
persistent identity: range2501710
display id: range2501710
version: 1
direction: direction/reverse
top level: BBa_K1992005
start: 44
end: 67
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2501708
display id: annotation2501708
version: 1
top level: BBa_K1992005
location: https://synbiohub.org/public/igem/BBa_K1992005/annotation2501708/range2501708/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1992005/annotation2501708/range2501708/1
type: Range
persistent identity: range2501708
display id: range2501708
version: 1
direction: direction/reverse
top level: BBa_K1992005
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2501714
display id: annotation2501714
version: 1
top level: BBa_K1992005
location: https://synbiohub.org/public/igem/BBa_K1992005/annotation2501714/range2501714/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K1992005/annotation2501714/range2501714/1
type: Range
persistent identity: range2501714
display id: range2501714
version: 1
direction: direction/reverse
top level: BBa_K1992005
start: 74
end: 1735
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2501721
display id: annotation2501721
version: 1
top level: BBa_K1992005
location: https://synbiohub.org/public/igem/BBa_K1992005/annotation2501721/range2501721/1
component: BBa_B0015

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type: Range
persistent identity: range2501721
display id: range2501721
version: 1
direction: direction/reverse
top level: BBa_K1992005
start: 1744
end: 1872
orientation: inline

BBa_K209401
type: ComponentDefinition
persistent identity: BBa K209401
display id: BBa_K209401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI B-C part, Rs1.3
creator: Katja Kolar
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:27Z
discontinued: false
dominant: false
group u list: _312_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Rs1.3 RASSL flanked by AarI BC sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K209401_sequence/1

BBa_J07009
type: ComponentDefinition
persistent identity: BBa J07009
display id: BBa_J07009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: toxicity-gene activator from Vibrio cholerae
creator: mit_igem_2005
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: In stock
status: status/Available
bookmark: true
mutable description: shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done @ aa 211. explanation of ToxR domains @ http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf ELIMINATE XbaI SITE PLZZZZZ
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Periplasmic Region
sequence annotation: Transmembrane Region
sequence annotation: Cytoplasmic Region
sequence annotation: Coding Sequence for ToxR'
sequence: https://synbiohub.org/public/igem/BBa_J07009_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation1668508
display id: annotation1668508
version: 1
top level: BBa_J07009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668508/range1668508/1
type: Range
persistent identity: range1668508
display id: range1668508
version: 1
direction: direction/forward
top level: BBa_J07009
start: 6
end: 8
orientation: inline

Periplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668510
display id: annotation1668510
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668510/range1668510/1
type: Range
persistent identity: range1668510
display id: range1668510
version: 1
direction: direction/forward
top level: BBa_J07009
start: 595
end: 630
orientation: inline

Transmembrane Region
type: SequenceAnnotation
persistent identity: annotation1668509
display id: annotation1668509
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668509/range1668509/1
type: Range
persistent identity: range1668509
display id: range1668509
version: 1
direction: direction/forward
top level: BBa_J07009
start: 547
end: 594
orientation: inline

Cytoplasmic Region
type: SequenceAnnotation
persistent identity: annotation1668506
display id: annotation1668506
version: 1
top level: BBa_J07009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668506/range1668506/1
type: Range
persistent identity: range1668506
display id: range1668506
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 546
orientation: inline

Coding Sequence for ToxR'
type: SequenceAnnotation
persistent identity: annotation1668507
display id: annotation1668507
version: 1
top level: BBa_J07009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1

https://synbiohub.org/public/igem/BBa_J07009/annotation1668507/range1668507/1
type: Range
persistent identity: range1668507
display id: range1668507
version: 1
direction: direction/forward
top level: BBa_J07009
start: 1
end: 630
orientation: inline

BBa_K786001
type: ComponentDefinition
persistent identity: BBa K786001
display id: BBa_K786001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: linker
sequence annotation: HindIII for N-terminal fusin
sequence annotation: BamHI for C-terminal fusion
sequence annotation: HtrII
sequence annotation: RBS 1.0
sequence annotation: B0015
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: Sensory Rhodopsin II
sequence annotation: Tsr
sequence: https://synbiohub.org/public/igem/BBa_K786001_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation2199668
display id: annotation2199668
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1
type: Range
persistent identity: range2199668
display id: range2199668
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 811
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199666
display id: annotation2199666
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1
type: Range
persistent identity: range2199666
display id: range2199666
version: 1
direction: direction/forward
top level: BBa_K786001
start: 62
end: 67
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199669
display id: annotation2199669
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1
type: Range
persistent identity: range2199669
display id: range2199669
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 790
orientation: inline

HtrII
type: SequenceAnnotation
persistent identity: annotation2199670
display id: annotation2199670
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1
type: Range
persistent identity: range2199670
display id: range2199670
version: 1
direction: direction/forward
top level: BBa_K786001
start: 812
end: 1210
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199665
display id: annotation2199665
version: 1
top level: BBa_K786001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1
type: Range
persistent identity: range2199665
display id: range2199665
version: 1
direction: direction/forward
top level: BBa_K786001
start: 43
end: 55
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199672
display id: annotation2199672
version: 1
top level: BBa_K786001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1
type: Range
persistent identity: range2199672
display id: range2199672
version: 1
direction: direction/forward
top level: BBa_K786001
start: 2105
end: 2233
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199664
display id: annotation2199664
version: 1
top level: BBa_K786001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1
type: Range
persistent identity: range2199664
display id: range2199664
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1
end: 42
orientation: inline

Sensory Rhodopsin II
type: SequenceAnnotation
persistent identity: annotation2199667
display id: annotation2199667
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1
type: Range
persistent identity: range2199667
display id: range2199667
version: 1
direction: direction/forward
top level: BBa_K786001
start: 68
end: 784
orientation: inline

Tsr
type: SequenceAnnotation
persistent identity: annotation2199671
display id: annotation2199671
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1
type: Range
persistent identity: range2199671
display id: range2199671
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1210
end: 2070
orientation: inline

BBa_K533008
type: ComponentDefinition
persistent identity: BBa K533008
display id: BBa_K533008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAA receptor
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant version of E. Coli Tar receptor and changes the chemotaxis properties. This receptor now responds to phenylacetic acid (PAA).
mutable notes: LacI promoter without Shine-Dalgarno sequence ensures sensitive and specific chemotaxis
mutable provenance: This is a kind gift from Dr. Goulian.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PAA Receptor
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K533008_sequence/1

PAA Receptor
type: SequenceAnnotation
persistent identity: annotation2152018
display id: annotation2152018
version: 1
top level: BBa_K533008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533008/annotation2152018/range2152018/1

https://synbiohub.org/public/igem/BBa_K533008/annotation2152018/range2152018/1
type: Range
persistent identity: range2152018
display id: range2152018
version: 1
direction: direction/forward
top level: BBa_K533008
start: 1
end: 1662
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2152019
display id: annotation2152019
version: 1
top level: BBa_K533008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533008/annotation2152019/range2152019/1

https://synbiohub.org/public/igem/BBa_K533008/annotation2152019/range2152019/1
type: Range
persistent identity: range2152019
display id: range2152019
version: 1
direction: direction/forward
top level: BBa_K533008
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152017
display id: annotation2152017
version: 1
top level: BBa_K533008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533008/annotation2152017/range2152017/1

https://synbiohub.org/public/igem/BBa_K533008/annotation2152017/range2152017/1
type: Range
persistent identity: range2152017
display id: range2152017
version: 1
direction: direction/forward
top level: BBa_K533008
start: 1660
end: 1662
orientation: inline

VirA/G
type: ComponentDefinition
persistent identity: BBa K389017
display id: BBa_K389017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system without read-out
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick is a virA/G signaling system without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter.
mutable notes: This BioBrick can be used to control any gene with a ''vir'' promoter
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0017
component: BBa_K389002
component: BBa_K389010
component: BBa_K389003
component: BBa_J23110
sequence annotation: BBa_K389003
sequence annotation: BBa_K389010
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_B0017
sequence: https://synbiohub.org/public/igem/BBa_K389017_sequence/1

BBa_B0017
type: Component
persistent identity: component2226706
display id: component2226706
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389002
type: Component
persistent identity: component2226701
display id: component2226701
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389010
type: Component
persistent identity: component2226696
display id: component2226696
version: 1
top level: VirA/G
access: public
definition: virA

BBa_K389003
type: Component
persistent identity: component2226711
display id: component2226711
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_J23110
type: Component
persistent identity: component2226697
display id: component2226697
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2226711
display id: annotation2226711
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
type: Range
persistent identity: range2226711
display id: range2226711
version: 1
direction: direction/reverse
top level: VirA/G
start: 3542
end: 3699
orientation: inline

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2226696
display id: annotation2226696
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226696/range2226696/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389017/annotation2226696/range2226696/1
type: Range
persistent identity: range2226696
display id: range2226696
version: 1
direction: direction/reverse
top level: VirA/G
start: 1
end: 2566
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2226701
display id: annotation2226701
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226701/range2226701/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389017/annotation2226701/range2226701/1
type: Range
persistent identity: range2226701
display id: range2226701
version: 1
direction: direction/reverse
top level: VirA/G
start: 2618
end: 3357
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2226697
display id: annotation2226697
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226697/range2226697/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389017/annotation2226697/range2226697/1
type: Range
persistent identity: range2226697
display id: range2226697
version: 1
direction: direction/reverse
top level: VirA/G
start: 2575
end: 2609
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2226706
display id: annotation2226706
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226706/range2226706/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389017/annotation2226706/range2226706/1
type: Range
persistent identity: range2226706
display id: range2226706
version: 1
direction: direction/reverse
top level: VirA/G
start: 3366
end: 3533
orientation: inline

BBa_K1909008
type: ComponentDefinition
persistent identity: BBa K1909008
display id: BBa_K1909008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium Anderson / mTaz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:44:03Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: Coming soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K608006
component: BBa_K1909002
sequence annotation: BBa_K1909002
sequence annotation: BBa_K608006
sequence: https://synbiohub.org/public/igem/BBa_K1909008_sequence/1

BBa_K608006
type: Component
persistent identity: component2507591
display id: component2507591
version: 1
top level: BBa_K1909008
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K1909002
type: Component
persistent identity: component2507597
display id: component2507597
version: 1
top level: BBa_K1909008
access: public
definition: mTaz

BBa_K1909002
type: SequenceAnnotation
persistent identity: annotation2507597
display id: annotation2507597
version: 1
top level: BBa_K1909008
location: https://synbiohub.org/public/igem/BBa_K1909008/annotation2507597/range2507597/1
component: BBa_K1909002

https://synbiohub.org/public/igem/BBa_K1909008/annotation2507597/range2507597/1
type: Range
persistent identity: range2507597
display id: range2507597
version: 1
direction: direction/reverse
top level: BBa_K1909008
start: 63
end: 1514
orientation: inline

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2507591
display id: annotation2507591
version: 1
top level: BBa_K1909008
location: https://synbiohub.org/public/igem/BBa_K1909008/annotation2507591/range2507591/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1909008/annotation2507591/range2507591/1
type: Range
persistent identity: range2507591
display id: range2507591
version: 1
direction: direction/reverse
top level: BBa_K1909008
start: 1
end: 56
orientation: inline

virA
type: ComponentDefinition
persistent identity: BBa K389001
display id: BBa_K389001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA receptor
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis.
mutable notes: Illegal ''PstI'' restriction site removed
mutable provenance: ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: virA
sequence annotation: Stop codon
sequence annotation: Illegal PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K389001_sequence/1

virA
type: SequenceAnnotation
persistent identity: annotation2077044
display id: annotation2077044
version: 1
top level: virA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2077044/range2077044/1
type: Range
persistent identity: range2077044
display id: range2077044
version: 1
direction: direction/forward
top level: virA
start: 1
end: 2505
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078363
display id: annotation2078363
version: 1
top level: virA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2078363/range2078363/1
type: Range
persistent identity: range2078363
display id: range2078363
version: 1
direction: direction/forward
top level: virA
start: 2503
end: 2505
orientation: inline

Illegal PstI site removed
type: SequenceAnnotation
persistent identity: annotation2077043
display id: annotation2077043
version: 1
top level: virA
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1

https://synbiohub.org/public/igem/BBa_K389001/annotation2077043/range2077043/1
type: Range
persistent identity: range2077043
display id: range2077043
version: 1
direction: direction/forward
top level: virA
start: 187
end: 187
orientation: inline

BBa_K748001
type: ComponentDefinition
persistent identity: BBa K748001
display id: BBa_K748001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AgrC is a transmembrane protein, which is the sensor molecule of a typical two-component signal system in S.aureus. AgrC possesses several key amino acid motifs typical of histidine protein kinase sensor. The AgrC sensor kinase can specifically binds to corresponding AIP, which secreted only from specific S.aureus, and the composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.
mutable notes: we employed site-specific mutagenesis(AgrC-I A101T) to replace a amino acid residue in AgrC-I so that the mutated AgrC can bound to both AIP-I and AIP-IV.
mutable provenance: Staphylococcus aureus genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgrC-I A101T
sequence: https://synbiohub.org/public/igem/BBa_K748001_sequence/1

AgrC-I A101T
type: SequenceAnnotation
persistent identity: annotation2183341
display id: annotation2183341
version: 1
top level: BBa_K748001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K748001/annotation2183341/range2183341/1

https://synbiohub.org/public/igem/BBa_K748001/annotation2183341/range2183341/1
type: Range
persistent identity: range2183341
display id: range2183341
version: 1
direction: direction/forward
top level: BBa_K748001
start: 301
end: 303
orientation: inline

BBa_K133060
type: ComponentDefinition
persistent identity: BBa K133060
display id: BBa_K133060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TMTIR)TLR9
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Toll-Like Receptor 9 transmembrane region and cytoplasmic domain.
mutable provenance: amplified from TLR9 gene, obtained from Invivogen, plasmid pUNO-hTLR9
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133060_sequence/1

lamB
type: ComponentDefinition
persistent identity: BBa K137112
display id: BBa_K137112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lamB
creator: Fei Chen
created: 2008-09-23T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2988
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lamB is the a porin protein which is part of the maltose pathway. It is also the receptor associated and required fro lambda phage infection.
mutable notes: N/A
mutable provenance: DH10B Genomic DNA Prep
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137112_sequence/1

BBa_K845007
type: ComponentDefinition
persistent identity: BBa K845007
display id: BBa_K845007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ intracellular domain
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: envZ
sequence: https://synbiohub.org/public/igem/BBa_K845007_sequence/1

envZ
type: SequenceAnnotation
persistent identity: annotation2204296
display id: annotation2204296
version: 1
top level: BBa_K845007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K845007/annotation2204296/range2204296/1

https://synbiohub.org/public/igem/BBa_K845007/annotation2204296/range2204296/1
type: Range
persistent identity: range2204296
display id: range2204296
version: 1
direction: direction/forward
top level: BBa_K845007
start: 1
end: 684
orientation: inline

BBa_K533001
type: ComponentDefinition
persistent identity: BBa K533001
display id: BBa_K533001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-SH3
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the N-terminal 180 amino acids of OmpA fused to SH3 domain in human Grb2 protein. The N-terminal of OmpA allows the moiety fused to it to be presented on to the outer membrane of E. Coli. SH3 domain allows for binding to multi-proline peptide in human cells, which functions as a crucial adaptor module in signaling. In our part, this is specially for binding to the proteins for transportation by E. Coli. All the coding sequence is under T7 promoter and lac repressor binding site. The parts is most efficiently presented onto the membrane when induced at 0.1mM IPTG at 18 centigrade in BL21 E. Coli strain.
mutable notes: High expression efficiency as well as inducible expression is most desirable when considering a coding sequence.
mutable provenance: OmpA is derived from the genomic DNA of BL21 (DE3) E. Coli strain while SH3 is from human Grb2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: SH3
sequence annotation: OmpA
sequence: https://synbiohub.org/public/igem/BBa_K533001_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2150406
display id: annotation2150406
version: 1
top level: BBa_K533001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1
type: Range
persistent identity: range2150406
display id: range2150406
version: 1
direction: direction/forward
top level: BBa_K533001
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150407
display id: annotation2150407
version: 1
top level: BBa_K533001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1
type: Range
persistent identity: range2150407
display id: range2150407
version: 1
direction: direction/forward
top level: BBa_K533001
start: 1
end: 18
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2150409
display id: annotation2150409
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1
type: Range
persistent identity: range2150409
display id: range2150409
version: 1
direction: direction/forward
top level: BBa_K533001
start: 633
end: 815
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150408
display id: annotation2150408
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1
type: Range
persistent identity: range2150408
display id: range2150408
version: 1
direction: direction/forward
top level: BBa_K533001
start: 87
end: 632
orientation: inline

BBa_K1072018
type: ComponentDefinition
persistent identity: BBa K1072018
display id: BBa_K1072018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + Brho + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072014
component: BBa_K1072020
sequence annotation: BBa_K1072020
sequence annotation: BBa_K1072014
sequence: https://synbiohub.org/public/igem/BBa_K1072018_sequence/1

BBa_K1072014
type: Component
persistent identity: component2366672
display id: component2366672
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072014/1

BBa_K1072020
type: Component
persistent identity: component2366679
display id: component2366679
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072020/1

BBa_K1072020
type: SequenceAnnotation
persistent identity: annotation2366679
display id: annotation2366679
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
component: BBa_K1072020

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
type: Range
persistent identity: range2366679
display id: range2366679
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 2766
end: 4235
orientation: inline

BBa_K1072014
type: SequenceAnnotation
persistent identity: annotation2366672
display id: annotation2366672
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
component: BBa_K1072014

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
type: Range
persistent identity: range2366672
display id: range2366672
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 1
end: 2757
orientation: inline

BBa_K1909004
type: ComponentDefinition
persistent identity: BBa K1909004
display id: BBa_K1909004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOD1 LRR Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:21:00Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: coming soon
mutable provenance: NOD1 (ncbi: https://www.ncbi.nlm.nih.gov/gene/10392)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed CTG to TTG
sequence annotation: Changed GAC to GAT
sequence annotation: Changed CTG to CTA
sequence annotation: Changed CTA to TTA
sequence annotation: NOD1
sequence: https://synbiohub.org/public/igem/BBa_K1909004_sequence/1

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507292
display id: annotation2507292
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1
type: Range
persistent identity: range2507292
display id: range2507292
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 700
end: 702
orientation: inline

Changed GAC to GAT
type: SequenceAnnotation
persistent identity: annotation2507200
display id: annotation2507200
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1
type: Range
persistent identity: range2507200
display id: range2507200
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 181
end: 183
orientation: inline

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507291
display id: annotation2507291
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1
type: Range
persistent identity: range2507291
display id: range2507291
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 277
end: 279
orientation: inline

Changed CTG to CTA
type: SequenceAnnotation
persistent identity: annotation2507294
display id: annotation2507294
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1
type: Range
persistent identity: range2507294
display id: range2507294
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 727
end: 729
orientation: inline

Changed CTA to TTA
type: SequenceAnnotation
persistent identity: annotation2507293
display id: annotation2507293
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1
type: Range
persistent identity: range2507293
display id: range2507293
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 718
end: 720
orientation: inline

NOD1
type: SequenceAnnotation
persistent identity: annotation2507199
display id: annotation2507199
version: 1
top level: BBa_K1909004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1
type: Range
persistent identity: range2507199
display id: range2507199
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 1
end: 969
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: SRI-HtrI-Tar
sequence annotation: RBS 1.0
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

BBa_I712010
type: ComponentDefinition
persistent identity: BBa I712010
display id: BBa_I712010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4 sequence without signal peptide
creator: peter cimermancic
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1850
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It`s a chemokine receptor protein.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712010_sequence/1

BBa_K909008
type: ComponentDefinition
persistent identity: BBa K909008
display id: BBa_K909008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR-DBD-UVR8 fusion protein
creator: Gintautas Vainorius
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
group u list: _1174_
m user id: 0
owner id: 14094
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
mutable notes: Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion
mutable provenance: tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10 cDNA of UVR8 was provided by Roman Ulm, University of Geneva.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR-DBD
sequence annotation: UVR8
sequence: https://synbiohub.org/public/igem/BBa_K909008_sequence/1

tetR-DBD
type: SequenceAnnotation
persistent identity: annotation2194628
display id: annotation2194628
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194628/range2194628/1
type: Range
persistent identity: range2194628
display id: range2194628
version: 1
direction: direction/forward
top level: BBa_K909008
start: 1
end: 390
orientation: inline

UVR8
type: SequenceAnnotation
persistent identity: annotation2194629
display id: annotation2194629
version: 1
top level: BBa_K909008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1

https://synbiohub.org/public/igem/BBa_K909008/annotation2194629/range2194629/1
type: Range
persistent identity: range2194629
display id: range2194629
version: 1
direction: direction/forward
top level: BBa_K909008
start: 391
end: 1674
orientation: inline

BBa_K133064
type: ComponentDefinition
persistent identity: BBa K133064
display id: BBa_K133064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TIR)TLR9
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Toll-Like Receptor 9 cytoplasmic domain. Cloned into pSB1AK3 vector.
mutable provenance: amplified from TLR9 gene, obtained from Invivogen, plasmid pUNO-hTLR9
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133064_sequence/1

BBa_K533010
type: ComponentDefinition
persistent identity: BBa K533010
display id: BBa_K533010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAA receptor weakened expression cassette
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lower expression level of PAA receptor
mutable notes: Lower the expression efficiency.
mutable provenance: Gift from Dr. Goulian
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: lacI stop
sequence annotation: PAA Receptor
sequence annotation: bla
sequence annotation: weak -35
sequence annotation: -35
sequence annotation: weak -10
sequence annotation: incomplete lacI
sequence: https://synbiohub.org/public/igem/BBa_K533010_sequence/1

lacI stop
type: SequenceAnnotation
persistent identity: annotation2152086
display id: annotation2152086
version: 1
top level: BBa_K533010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152086/range2152086/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152086/range2152086/1
type: Range
persistent identity: range2152086
display id: range2152086
version: 1
direction: direction/forward
top level: BBa_K533010
start: 660
end: 662
orientation: inline

PAA Receptor
type: SequenceAnnotation
persistent identity: annotation2152079
display id: annotation2152079
version: 1
top level: BBa_K533010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152079/range2152079/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152079/range2152079/1
type: Range
persistent identity: range2152079
display id: range2152079
version: 1
direction: direction/forward
top level: BBa_K533010
start: 655
end: 2316
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2152080
display id: annotation2152080
version: 1
top level: BBa_K533010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152080/range2152080/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152080/range2152080/1
type: Range
persistent identity: range2152080
display id: range2152080
version: 1
direction: direction/forward
top level: BBa_K533010
start: 2867
end: 3727
orientation: inline

weak -35
type: SequenceAnnotation
persistent identity: annotation2152078
display id: annotation2152078
version: 1
top level: BBa_K533010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152078/range2152078/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152078/range2152078/1
type: Range
persistent identity: range2152078
display id: range2152078
version: 1
direction: direction/forward
top level: BBa_K533010
start: 565
end: 570
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2152087
display id: annotation2152087
version: 1
top level: BBa_K533010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152087/range2152087/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152087/range2152087/1
type: Range
persistent identity: range2152087
display id: range2152087
version: 1
direction: direction/forward
top level: BBa_K533010
start: 85
end: 90
orientation: inline

weak -10
type: SequenceAnnotation
persistent identity: annotation2146981
display id: annotation2146981
version: 1
top level: BBa_K533010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2146981/range2146981/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2146981/range2146981/1
type: Range
persistent identity: range2146981
display id: range2146981
version: 1
direction: direction/forward
top level: BBa_K533010
start: 585
end: 590
orientation: inline

incomplete lacI
type: SequenceAnnotation
persistent identity: annotation2152085
display id: annotation2152085
version: 1
top level: BBa_K533010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533010/annotation2152085/range2152085/1

https://synbiohub.org/public/igem/BBa_K533010/annotation2152085/range2152085/1
type: Range
persistent identity: range2152085
display id: range2152085
version: 1
direction: direction/forward
top level: BBa_K533010
start: 165
end: 662
orientation: inline

BBa_K1909010
type: ComponentDefinition
persistent identity: BBa K1909010
display id: BBa_K1909010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:44:31Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K081005
component: BBa_C0082
sequence annotation: BBa_K081005
sequence annotation: BBa_C0082
sequence: https://synbiohub.org/public/igem/BBa_K1909010_sequence/1

BBa_K081005
type: Component
persistent identity: component2508772
display id: component2508772
version: 1
top level: BBa_K1909010
access: public
definition: https://synbiohub.org/public/igem/BBa_K081005/1

BBa_C0082
type: Component
persistent identity: component2508778
display id: component2508778
version: 1
top level: BBa_K1909010
access: public
definition: tar-envZ

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2508772
display id: annotation2508772
version: 1
top level: BBa_K1909010
location: https://synbiohub.org/public/igem/BBa_K1909010/annotation2508772/range2508772/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K1909010/annotation2508772/range2508772/1
type: Range
persistent identity: range2508772
display id: range2508772
version: 1
direction: direction/reverse
top level: BBa_K1909010
start: 1
end: 58
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508778
display id: annotation2508778
version: 1
top level: BBa_K1909010
location: https://synbiohub.org/public/igem/BBa_K1909010/annotation2508778/range2508778/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909010/annotation2508778/range2508778/1
type: Range
persistent identity: range2508778
display id: range2508778
version: 1
direction: direction/reverse
top level: BBa_K1909010
start: 65
end: 1580
orientation: inline

BBa_I712002
type: ComponentDefinition
persistent identity: BBa I712002
display id: BBa_I712002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCR5
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (C-C motif) receptor 5, a HIV-1 co-receptor.
mutable notes: The part is cloned into BBa_J52017, a vector with eukaryontic terminator. PstI and EcoRI restriction sites inside CCR5 gene were mutated.
mutable provenance: CCR5 gene was obtained from Origene.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CCR5
sequence: https://synbiohub.org/public/igem/BBa_I712002_sequence/1

CCR5
type: SequenceAnnotation
persistent identity: annotation1951768
display id: annotation1951768
version: 1
top level: BBa_I712002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1

https://synbiohub.org/public/igem/BBa_I712002/annotation1951768/range1951768/1
type: Range
persistent identity: range1951768
display id: range1951768
version: 1
direction: direction/forward
top level: BBa_I712002
start: 1
end: 1059
orientation: inline

BBa_K389421
type: ComponentDefinition
persistent identity: BBa K389421
display id: BBa_K389421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389401
component: BBa_K389017
sequence annotation: BBa_K389017
sequence annotation: BBa_K389401
sequence: https://synbiohub.org/public/igem/BBa_K389421_sequence/1

BBa_K389401
type: Component
persistent identity: component2313872
display id: component2313872
version: 1
top level: BBa_K389421
access: public
definition: https://synbiohub.org/public/igem/BBa_K389401/1

BBa_K389017
type: Component
persistent identity: component2313848
display id: component2313848
version: 1
top level: BBa_K389421
access: public
definition: VirA/G

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313848
display id: annotation2313848
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313848/range2313848/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389421/annotation2313848/range2313848/1
type: Range
persistent identity: range2313848
display id: range2313848
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 1
end: 3699
orientation: inline

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2313872
display id: annotation2313872
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313872/range2313872/1
component: BBa_K389401

https://synbiohub.org/public/igem/BBa_K389421/annotation2313872/range2313872/1
type: Range
persistent identity: range2313872
display id: range2313872
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 3708
end: 6594
orientation: inline

BBa_I712012
type: ComponentDefinition
persistent identity: BBa I712012
display id: BBa_I712012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TIR domain of TLR3
creator: Andrej Ondracka
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Toll-like receptor 3 cytoplasmic domain.
mutable notes: Cloned into pSBAK3 vector with terminator. Start codon is NOT included.
mutable provenance: amplified from TLR3 gene, obtained from
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TIR domain
sequence: https://synbiohub.org/public/igem/BBa_I712012_sequence/1

TIR domain
type: SequenceAnnotation
persistent identity: annotation1951817
display id: annotation1951817
version: 1
top level: BBa_I712012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1

https://synbiohub.org/public/igem/BBa_I712012/annotation1951817/range1951817/1
type: Range
persistent identity: range1951817
display id: range1951817
version: 1
direction: direction/forward
top level: BBa_I712012
start: 1
end: 456
orientation: inline

BBa_K845006
type: ComponentDefinition
persistent identity: BBa K845006
display id: BBa_K845006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tap extracellular domain
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tap
sequence: https://synbiohub.org/public/igem/BBa_K845006_sequence/1

Tap
type: SequenceAnnotation
persistent identity: annotation2204295
display id: annotation2204295
version: 1
top level: BBa_K845006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K845006/annotation2204295/range2204295/1

https://synbiohub.org/public/igem/BBa_K845006/annotation2204295/range2204295/1
type: Range
persistent identity: range2204295
display id: range2204295
version: 1
direction: direction/forward
top level: BBa_K845006
start: 1
end: 765
orientation: inline

BBa_K1587008
type: ComponentDefinition
persistent identity: BBa K1587008
display id: BBa_K1587008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light biosensor
creator: Alexandre Le Scornet
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T02:51:05Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2004_
m user id: 24598
owner id: 24598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Strong promoter BBa_J23119. Chimeric Red light receptor cph8 BBa_I15010 (cph1-envZ) and proteins involved in phycobilin biosynthesis ho1 (BBa_I15008), pcyA (BBa_I15009).
mutable notes: No information
mutable provenance: Sequences are from Cyanobacteria : - Cph1 domain - ho1 BBa_I15008 - pcyA BBa_I15009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Strong promoter
sequence annotation: rbs
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K1587008_sequence/1

Strong promoter
type: SequenceAnnotation
persistent identity: annotation2466980
display id: annotation2466980
version: 1
top level: BBa_K1587008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1
type: Range
persistent identity: range2466980
display id: range2466980
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 1
end: 35
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2466981
display id: annotation2466981
version: 1
top level: BBa_K1587008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1
type: Range
persistent identity: range2466981
display id: range2466981
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 36
end: 50
orientation: inline

cph8
type: SequenceAnnotation
persistent identity: annotation2466982
display id: annotation2466982
version: 1
top level: BBa_K1587008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1
type: Range
persistent identity: range2466982
display id: range2466982
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 51
end: 2288
orientation: inline

BBa_K259001
type: ComponentDefinition
persistent identity: BBa K259001
display id: BBa_K259001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fiu ??? Outer Membrane Ferric Iron Transporter
creator: Petros Mina
created: 2009-07-13T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ===Biology of Fiu=== The fiu gene encodes for an outer membrane transporter found in E.coli, a gram negative bacterium. It is involved in the active transport (energy dependent uptake) of ferric iron from the extracellular environment. This outer membrane receptor can only bind ferric iron and is a catecholate siderophore. For uptake into the cell to be completed fiu has to complex with another protein known as TonB (a transperiplasmic protein) that will carry out the active transport by using ATP as an energy source. ===What this Biobrick gives you upon expression=== This biobrick encodes ONLY for fiu. ===Fiu Statistics=== Fiu belongs to the family of Ton-B dependent receptors and is related to fhuA, hence it also has a beta-barrel structure. {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure |- |E.Coli |Monomer |760/81.96kDa |Outer Membrane/OMV |beta-barrel |} ===References=== [http://ecoliwiki.net/colipedia/index.php/fiu:Gene_Product%28s%29#Domains.2FMotifs.2FModification_Sites E.Coli wiki net] Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner) [http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Ecogene number EG13317] [http://www.uniprot.org/uniprot/P75780 UniProt Accession number P75780]
mutable notes: In order to comply with assembly standard 10 (RFC10) the stop codon for fiu had to be changed to TAA.
mutable provenance: Fiu gene is part of the chromosomal E.coli genome. In particular it is found at 18.07min with orientation in the counter-clockwise direction. [http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Further details at EcoGene]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Signal Sequence
sequence annotation: Multipass protein (OM-Periplasm)
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K259001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2011313
display id: annotation2011313
version: 1
top level: BBa_K259001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011313/range2011313/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011313/range2011313/1
type: Range
persistent identity: range2011313
display id: range2011313
version: 1
direction: direction/forward
top level: BBa_K259001
start: 1
end: 3
orientation: inline

Signal Sequence
type: SequenceAnnotation
persistent identity: annotation2011315
display id: annotation2011315
version: 1
top level: BBa_K259001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011315/range2011315/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011315/range2011315/1
type: Range
persistent identity: range2011315
display id: range2011315
version: 1
direction: direction/forward
top level: BBa_K259001
start: 1
end: 99
orientation: inline

Multipass protein (OM-Periplasm)
type: SequenceAnnotation
persistent identity: annotation2011316
display id: annotation2011316
version: 1
top level: BBa_K259001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011316/range2011316/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011316/range2011316/1
type: Range
persistent identity: range2011316
display id: range2011316
version: 1
direction: direction/forward
top level: BBa_K259001
start: 100
end: 2247
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2011314
display id: annotation2011314
version: 1
top level: BBa_K259001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K259001/annotation2011314/range2011314/1

https://synbiohub.org/public/igem/BBa_K259001/annotation2011314/range2011314/1
type: Range
persistent identity: range2011314
display id: range2011314
version: 1
direction: direction/forward
top level: BBa_K259001
start: 2243
end: 2247
orientation: inline

BBa_K976002
type: ComponentDefinition
persistent identity: BBa K976002
display id: BBa_K976002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FGFR-1 + FRS2 Complex
creator: Amna Sohail
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Fibroblast Growth Factor Receptor-1 and Fibroblast Growth Factor Receptor Substrate 2 complex comprises a fusion protein complex that has the capability to mediate signal transduction. In melanocytes, this multi-protein complex mediate signal transduction to such pathways as the MAPK pathway, which regulate melanocyte proliferation, migration, and differentiation.
mutable notes: No specific design considerations were dealt with.
mutable provenance: The FGFR-1 protein sequence has been derived from the Emblem Database. The FRS2 protein sequence has been derived from the UnitProt Database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K976000
component: BBa_K976001
sequence annotation: BBa_K976000
sequence annotation: BBa_K976001
sequence: https://synbiohub.org/public/igem/BBa_K976002_sequence/1

BBa_K976000
type: Component
persistent identity: component2329799
display id: component2329799
version: 1
top level: BBa_K976002
access: public
definition: FGFR-1

BBa_K976001
type: Component
persistent identity: component2329800
display id: component2329800
version: 1
top level: BBa_K976002
access: public
definition: https://synbiohub.org/public/igem/BBa_K976001/1

BBa_K976000
type: SequenceAnnotation
persistent identity: annotation2329799
display id: annotation2329799
version: 1
top level: BBa_K976002
location: https://synbiohub.org/public/igem/BBa_K976002/annotation2329799/range2329799/1
component: BBa_K976000

https://synbiohub.org/public/igem/BBa_K976002/annotation2329799/range2329799/1
type: Range
persistent identity: range2329799
display id: range2329799
version: 1
direction: direction/reverse
top level: BBa_K976002
start: 1
end: 2466
orientation: inline

BBa_K976001
type: SequenceAnnotation
persistent identity: annotation2329800
display id: annotation2329800
version: 1
top level: BBa_K976002
location: https://synbiohub.org/public/igem/BBa_K976002/annotation2329800/range2329800/1
component: BBa_K976001

https://synbiohub.org/public/igem/BBa_K976002/annotation2329800/range2329800/1
type: Range
persistent identity: range2329800
display id: range2329800
version: 1
direction: direction/reverse
top level: BBa_K976002
start: 2473
end: 3996
orientation: inline

BBa_K1909011
type: ComponentDefinition
persistent identity: BBa K1909011
display id: BBa_K1909011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium Anderson / Taz
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:45:57Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming Soon
mutable notes: Coming Soon
mutable provenance: Composite Part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K608006
component: BBa_C0082
sequence annotation: BBa_K608006
sequence annotation: BBa_C0082
sequence: https://synbiohub.org/public/igem/BBa_K1909011_sequence/1

BBa_K608006
type: Component
persistent identity: component2508783
display id: component2508783
version: 1
top level: BBa_K1909011
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_C0082
type: Component
persistent identity: component2508789
display id: component2508789
version: 1
top level: BBa_K1909011
access: public
definition: tar-envZ

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2508783
display id: annotation2508783
version: 1
top level: BBa_K1909011
location: https://synbiohub.org/public/igem/BBa_K1909011/annotation2508783/range2508783/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1909011/annotation2508783/range2508783/1
type: Range
persistent identity: range2508783
display id: range2508783
version: 1
direction: direction/reverse
top level: BBa_K1909011
start: 1
end: 56
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation2508789
display id: annotation2508789
version: 1
top level: BBa_K1909011
location: https://synbiohub.org/public/igem/BBa_K1909011/annotation2508789/range2508789/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_K1909011/annotation2508789/range2508789/1
type: Range
persistent identity: range2508789
display id: range2508789
version: 1
direction: direction/reverse
top level: BBa_K1909011
start: 63
end: 1578
orientation: inline

BBa_I712017
type: ComponentDefinition
persistent identity: BBa I712017
display id: BBa_I712017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.
mutable notes: CXCR4 and NUb fragments were fused with PCR overlap extension method without any linker added. The part is cloned into BBa_J52017 (a vector with eukaryontic terminator).
mutable provenance: CXCR4 gene obtained from Origene, NUb gene obtained from Dualsystems.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NUb
sequence annotation: CXCR4
sequence: https://synbiohub.org/public/igem/BBa_I712017_sequence/1

NUb
type: SequenceAnnotation
persistent identity: annotation1951575
display id: annotation1951575
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951575/range1951575/1
type: Range
persistent identity: range1951575
display id: range1951575
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1057
end: 1191
orientation: inline

CXCR4
type: SequenceAnnotation
persistent identity: annotation1951574
display id: annotation1951574
version: 1
top level: BBa_I712017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1

https://synbiohub.org/public/igem/BBa_I712017/annotation1951574/range1951574/1
type: Range
persistent identity: range1951574
display id: range1951574
version: 1
direction: direction/forward
top level: BBa_I712017
start: 1
end: 1056
orientation: inline

trz
type: ComponentDefinition
persistent identity: BBa J58104
display id: BBa_J58104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein Trg-EnvZ for signal transduction
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We created a hybrid gene coding for a protein in which Trg (chemoreceptor) contributed its periplasmic and transmembrane domains as well as a short cytoplasmic segment and EnvZ (osmosensor) contributed its cytoplasmic kinase/phosphatase domain.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58104_sequence/1

cph8
type: ComponentDefinition
persistent identity: BBa K592000
display id: BBa_K592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is identical to BBa_I15010, the cph8 red light sensor. We were unable to transform BBa_I15010 from the the 2011 iGEM distribution kit. PCR attempts using VF2 and VR failed to amplify any DNA from the well sample. In the end, we chose to amplify cph8 from Jeffrey Tabor's pJT122 plasmid provided by Chris Voigt. The cph8 in pJT122 contained an internal PstI site, which was removed by point-mutagenesis. The resultant DNA sequence is identical to that of BBa_I15010.
mutable notes: The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
mutable provenance: Jeffrey Tabor's pJT122 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K592000_sequence/1

cph8
type: SequenceAnnotation
persistent identity: annotation2132074
display id: annotation2132074
version: 1
top level: cph8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1

https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1
type: Range
persistent identity: range2132074
display id: range2132074
version: 1
direction: direction/forward
top level: cph8
start: 1
end: 2238
orientation: inline

mTaz
type: ComponentDefinition
persistent identity: BBa K1909002
display id: BBa_K1909002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mTaz (Taz-derived mDAP receptor)
creator: Daniel Wedemeyer, Alp Mirdoğan
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T10:47:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming as soon as we validated mTaz.
mutable notes: meso-2,6-Diaminopimelic acid (mDAP) is a non-proteinogenic amino acid that is synthesized and se-creted by Chlamydia trachomatis. mDAP has a relatively high chemical similarity to aspartate, sharing a Tanimoto coefficient of T=0.8 in a molecular fingerprint analysis [T<0: chemically very different struc-tures; T=1: identity]. As a proof of concept, a molecular docking model proposes few mutations which would change the Tar specificity towards mDAP. As input for molecular docking we used the crys-tal structure of the ligand binding domain of Tar bound to aspartate (PDB: 4Z9H). As a base for the part sequence, the BioBrick representation of the original Taz, BBa_C0082, was used. The BBa_C0082 BioBrick features a small part of the vector (64 nucleotides) it was derived from on its 3??? end, which we have removed along with the stop codon inside the Part for further fusion protein de-sign in RFC 12, 21, 23 and 25 assembly standards. We used the Glide algorithm, Maestro and the Schrodinger Suite for molecular docking of aspartate and mDAP to the Tar ligand binding domain (PDB: 4Z9H). Because of its structural similarity to aspartate, mDAP showed some affinity to the native Tar aspartate binding site (Table 1). Since mDAP is larger than aspartate, Y149 led to structural interference, not allowing mDAP to fully enter the binding site. To create the additional space needed for mDAP binding, we substituted Y149 with smaller amino acids. The best results were obtained with serine. Because of the additional size of mDAP, the bigger challenge was to reduce the affinity of aspartate rather than to raise the affinity of mDAP. We identified R64 as a key residue for aspartate binding which hovewer was not needed to bind mDAP. To minimally interfere with folding and function we performed a constitutive substitution and substituted it with lysine, creating the R64K Y149S double mutant called mTaz hereafter. Using mTaz, a higher docking score was achieved for mDAP than for aspartate (…
mutable provenance: mTaz is based on the Tar-EnvZ chimeric receptor which is capable of detecting aspartate in the medium as described by Utsumi et al. 1989. Tar, or methyl-accepting chemotaxis protein II, is a signal transducer in Escherichia coli chemotaxis and is activated upon the recognition of aspartate. Tar is sensitive to mutations altering its ligand specificity which sometimes results in recognizing other amino acids. EnvZ is a part of the well-studied and commonly used two-component system EnvZ/OmpR in E. coli, offering a simple way of signal transduction. Its periplasmic domain was shown to be exchangeable with different sensor domains e.g. Tar/EnvZ chimeric receptor capable of detecting aspartate in the medium.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Y149S
sequence annotation: Tar
sequence annotation: R64K
sequence annotation: EnvZ
sequence annotation: mTaz
sequence: https://synbiohub.org/public/igem/BBa_K1909002_sequence/1

Y149S
type: SequenceAnnotation
persistent identity: annotation2506924
display id: annotation2506924
version: 1
top level: mTaz
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506924/range2506924/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506924/range2506924/1
type: Range
persistent identity: range2506924
display id: range2506924
version: 1
direction: direction/forward
top level: mTaz
start: 445
end: 447
orientation: inline

Tar
type: SequenceAnnotation
persistent identity: annotation2506919
display id: annotation2506919
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506919/range2506919/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506919/range2506919/1
type: Range
persistent identity: range2506919
display id: range2506919
version: 1
direction: direction/forward
top level: mTaz
start: 1
end: 765
orientation: inline

R64K
type: SequenceAnnotation
persistent identity: annotation2506923
display id: annotation2506923
version: 1
top level: mTaz
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506923/range2506923/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506923/range2506923/1
type: Range
persistent identity: range2506923
display id: range2506923
version: 1
direction: direction/forward
top level: mTaz
start: 190
end: 192
orientation: inline

EnvZ
type: SequenceAnnotation
persistent identity: annotation2506922
display id: annotation2506922
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506922/range2506922/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506922/range2506922/1
type: Range
persistent identity: range2506922
display id: range2506922
version: 1
direction: direction/forward
top level: mTaz
start: 766
end: 1452
orientation: inline

mTaz
type: SequenceAnnotation
persistent identity: annotation2506915
display id: annotation2506915
version: 1
top level: mTaz
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909002/annotation2506915/range2506915/1

https://synbiohub.org/public/igem/BBa_K1909002/annotation2506915/range2506915/1
type: Range
persistent identity: range2506915
display id: range2506915
version: 1
direction: direction/forward
top level: mTaz
start: 1
end: 1452
orientation: inline

BBa_K533011
type: ComponentDefinition
persistent identity: BBa K533011
display id: BBa_K533011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-HIV protease site-SH3 expression cassette
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: EM7 for efficient expression of SH3
mutable notes: EM7 is a synthesized promoter based on the consensus sequence of E. Coli promoter.
mutable provenance: OmpA-HIV protease site-SH3 from BBa_K533002 EM7 promoter is synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: OmpA-HIVsite-SH3
sequence annotation: OmpA
sequence annotation: BBa_I712015
sequence annotation: rbs
sequence annotation: -10
sequence annotation: -35
sequence annotation: SH3
sequence: https://synbiohub.org/public/igem/BBa_K533011_sequence/1

OmpA-HIVsite-SH3
type: SequenceAnnotation
persistent identity: annotation2146983
display id: annotation2146983
version: 1
top level: BBa_K533011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2146983/range2146983/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2146983/range2146983/1
type: Range
persistent identity: range2146983
display id: range2146983
version: 1
direction: direction/forward
top level: BBa_K533011
start: 86
end: 856
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2152196
display id: annotation2152196
version: 1
top level: BBa_K533011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2152196/range2152196/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2152196/range2152196/1
type: Range
persistent identity: range2152196
display id: range2152196
version: 1
direction: direction/forward
top level: BBa_K533011
start: 86
end: 632
orientation: inline

BBa_I712015
type: SequenceAnnotation
persistent identity: annotation2152198
display id: annotation2152198
version: 1
top level: BBa_K533011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2152198/range2152198/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2152198/range2152198/1
type: Range
persistent identity: range2152198
display id: range2152198
version: 1
direction: direction/forward
top level: BBa_K533011
start: 633
end: 692
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2152195
display id: annotation2152195
version: 1
top level: BBa_K533011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2152195/range2152195/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2152195/range2152195/1
type: Range
persistent identity: range2152195
display id: range2152195
version: 1
direction: direction/forward
top level: BBa_K533011
start: 74
end: 79
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2146984
display id: annotation2146984
version: 1
top level: BBa_K533011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2146984/range2146984/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2146984/range2146984/1
type: Range
persistent identity: range2146984
display id: range2146984
version: 1
direction: direction/forward
top level: BBa_K533011
start: 27
end: 31
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2146982
display id: annotation2146982
version: 1
top level: BBa_K533011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2146982/range2146982/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2146982/range2146982/1
type: Range
persistent identity: range2146982
display id: range2146982
version: 1
direction: direction/forward
top level: BBa_K533011
start: 4
end: 10
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2152197
display id: annotation2152197
version: 1
top level: BBa_K533011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533011/annotation2152197/range2152197/1

https://synbiohub.org/public/igem/BBa_K533011/annotation2152197/range2152197/1
type: Range
persistent identity: range2152197
display id: range2152197
version: 1
direction: direction/forward
top level: BBa_K533011
start: 693
end: 856
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: Cph8
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
direction: direction/forward
top level: BBa_I15010
start: 2239
end: 2263
orientation: inline

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
direction: direction/forward
top level: BBa_I15010
start: 1
end: 2238
orientation: inline

BBa_K950006
type: ComponentDefinition
persistent identity: BBa K950006
display id: BBa_K950006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: membrane progesterone receptor (Danio rerio)
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Membrane progesterone receptor of Danio rerio. Upon ligand binding, the receptor represses expression of Fet3 in yeast.
mutable notes: restriction sites removed and codon usage optimized for yeast
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/AAN78115.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: mPR Danio rerio
sequence: https://synbiohub.org/public/igem/BBa_K950006_sequence/1

mPR Danio rerio
type: SequenceAnnotation
persistent identity: annotation2179275
display id: annotation2179275
version: 1
top level: BBa_K950006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950006/annotation2179275/range2179275/1

https://synbiohub.org/public/igem/BBa_K950006/annotation2179275/range2179275/1
type: Range
persistent identity: range2179275
display id: range2179275
version: 1
direction: direction/forward
top level: BBa_K950006
start: 1
end: 1065
orientation: inline

BBa_K772100
type: ComponentDefinition
persistent identity: BBa K772100
display id: BBa_K772100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Detection Device
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K772001
component: BBa_K772002
sequence annotation: BBa_K772001
sequence annotation: BBa_K772002
sequence: https://synbiohub.org/public/igem/BBa_K772100_sequence/1

BBa_K772001
type: Component
persistent identity: component2211341
display id: component2211341
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772001

BBa_K772002
type: Component
persistent identity: component2211348
display id: component2211348
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772002

BBa_K772001
type: SequenceAnnotation
persistent identity: annotation2211341
display id: annotation2211341
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
component: BBa_K772001

https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
type: Range
persistent identity: range2211341
display id: range2211341
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1
end: 1159
orientation: inline

BBa_K772002
type: SequenceAnnotation
persistent identity: annotation2211348
display id: annotation2211348
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
component: BBa_K772002

https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
type: Range
persistent identity: range2211348
display id: range2211348
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1168
end: 2305
orientation: inline

BBa_K133067
type: ComponentDefinition
persistent identity: BBa K133067
display id: BBa_K133067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (TMTIR)TLR4
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cloned into pSB1AK3 vector.
mutable provenance: amplified from TLR4 gene, obtained from Invivogen, plasmid pUNO-hTLR4
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133067_sequence/1

BBa_K389016
type: ComponentDefinition
persistent identity: BBa K389016
display id: BBa_K389016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G reporter device mRFP
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is for testing the virA/G signaling system. The read-out is the mRFP <partinfo>E1010</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
mutable notes: this is only for measuring the natural virA/G system
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389010
component: BBa_K389013
sequence annotation: BBa_K389013
sequence annotation: BBa_K389010
sequence: https://synbiohub.org/public/igem/BBa_K389016_sequence/1

BBa_K389010
type: Component
persistent identity: component2277648
display id: component2277648
version: 1
top level: BBa_K389016
access: public
definition: virA

BBa_K389013
type: Component
persistent identity: component2277667
display id: component2277667
version: 1
top level: BBa_K389016
access: public
definition: https://synbiohub.org/public/igem/BBa_K389013/1

BBa_K389013
type: SequenceAnnotation
persistent identity: annotation2277667
display id: annotation2277667
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
component: BBa_K389013

https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
type: Range
persistent identity: range2277667
display id: range2277667
version: 1
direction: direction/reverse
top level: BBa_K389016
start: 2575
end: 4411
orientation: inline

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2277648
display id: annotation2277648
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
type: Range
persistent identity: range2277648
display id: range2277648
version: 1
direction: direction/reverse
top level: BBa_K389016
start: 1
end: 2566
orientation: inline

BBa_K259010
type: ComponentDefinition
persistent identity: BBa K259010
display id: BBa_K259010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-FhuA
creator: Petros Mina
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==What this part gives upon expression== This part encodes for FhuA directly as it is already in frame with a pBAD/Arabinose regulated promoter. Promoter Induction can occur with 0.1% Arabinose in the culture environment. FhuA is an E.coli Outer Membrane (OM) protein used for scavenging iron from the nutrient medium. For more information on FhuA visit the FhuA main page (<partinfo>K259000</partinfo>). ==Potential uses of this Device== This part can be used also to create protein fusions by adding other proteins to it's 3' end. Protein fusion can be accomplished straightforwardly using the Bioscaffold-Linker biobricks <partinfo>K259003</partinfo>. Protein fusions with FhuA may allow delivery of proteins to the OM of E.coli cells or inclusion into Outer Membrane Vesicles (see iGEM'09 Bristol-BCCS project).
mutable notes: The part was assembled using standard RFC10 assembly.
mutable provenance: The arabinose promoter occurs naturally in E.coli. The RBS is designed from the Anderson family. FhuA also occurs naturally in E.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K259000
component: BBa_R0081
component: BBa_R0080
component: BBa_J61100
sequence annotation: BBa_K259000
sequence annotation: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_K259010_sequence/1

BBa_K259000
type: Component
persistent identity: component2037549
display id: component2037549
version: 1
top level: BBa_K259010
access: public
definition: fhuA

BBa_R0081
type: Component
persistent identity: component2037536
display id: component2037536
version: 1
top level: BBa_K259010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2037543
display id: component2037543
version: 1
top level: BBa_K259010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_J61100
type: Component
persistent identity: component2037544
display id: component2037544
version: 1
top level: BBa_K259010
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K259000
type: SequenceAnnotation
persistent identity: annotation2037549
display id: annotation2037549
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037549/range2037549/1
component: BBa_K259000

https://synbiohub.org/public/igem/BBa_K259010/annotation2037549/range2037549/1
type: Range
persistent identity: range2037549
display id: range2037549
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 367
end: 2613
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2037544
display id: annotation2037544
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037544/range2037544/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259010/annotation2037544/range2037544/1
type: Range
persistent identity: range2037544
display id: range2037544
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 349
end: 360
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2037536
display id: annotation2037536
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037536/range2037536/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259010/annotation2037536/range2037536/1
type: Range
persistent identity: range2037536
display id: range2037536
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2037543
display id: annotation2037543
version: 1
top level: BBa_K259010
location: https://synbiohub.org/public/igem/BBa_K259010/annotation2037543/range2037543/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259010/annotation2037543/range2037543/1
type: Range
persistent identity: range2037543
display id: range2037543
version: 1
direction: direction/reverse
top level: BBa_K259010
start: 192
end: 340
orientation: inline

BBa_K1660006
type: ComponentDefinition
persistent identity: BBa K1660006
display id: BBa_K1660006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-B0034-cph8
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:31:43Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the light sensor Cph8 with a promoter and RBS.
mutable notes: We constructed it through pcr.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592000
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K592000
sequence: https://synbiohub.org/public/igem/BBa_K1660006_sequence/1

BBa_K592000
type: Component
persistent identity: component2446442
display id: component2446442
version: 1
top level: BBa_K1660006
access: public
definition: cph8

BBa_B0034
type: Component
persistent identity: component2446440
display id: component2446440
version: 1
top level: BBa_K1660006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2446438
display id: component2446438
version: 1
top level: BBa_K1660006
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2446440
display id: annotation2446440
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
type: Range
persistent identity: range2446440
display id: range2446440
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2446438
display id: annotation2446438
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
type: Range
persistent identity: range2446438
display id: range2446438
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 1
end: 35
orientation: inline

BBa_K592000
type: SequenceAnnotation
persistent identity: annotation2446442
display id: annotation2446442
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
component: BBa_K592000

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
type: Range
persistent identity: range2446442
display id: range2446442
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 62
end: 2299
orientation: inline

VanBP
type: ComponentDefinition
persistent identity: BBa J58105
display id: BBa_J58105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic periplasmic binding protein that docks a vanillin molecule
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58105_sequence/1

BBa_I712003
type: ComponentDefinition
persistent identity: BBa I712003
display id: BBa_I712003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCR5-NUb
creator: Andrej Ondracka
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chemokine (C-C motif) receptor 5, fused to N-terminal half of ubiquitin.
mutable notes: PstI and EcoRI restriction sites inside CCR5 gene were mutated without changing the aminoacid sequence. CCR5 and NUb fragments were fused with PCR overlap extension method. The part is cloned into BBa_J52017, a vector with eukaryontic terminator.
mutable provenance: CCR5 gene obtained from Origene, NUb gene obtained from Dualsystems.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NUb
sequence annotation: CCR5
sequence: https://synbiohub.org/public/igem/BBa_I712003_sequence/1

NUb
type: SequenceAnnotation
persistent identity: annotation1950099
display id: annotation1950099
version: 1
top level: BBa_I712003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712003/annotation1950099/range1950099/1

https://synbiohub.org/public/igem/BBa_I712003/annotation1950099/range1950099/1
type: Range
persistent identity: range1950099
display id: range1950099
version: 1
direction: direction/forward
top level: BBa_I712003
start: 1057
end: 1194
orientation: inline

CCR5
type: SequenceAnnotation
persistent identity: annotation1950098
display id: annotation1950098
version: 1
top level: BBa_I712003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I712003/annotation1950098/range1950098/1

https://synbiohub.org/public/igem/BBa_I712003/annotation1950098/range1950098/1
type: Range
persistent identity: range1950098
display id: range1950098
version: 1
direction: direction/forward
top level: BBa_I712003
start: 1
end: 1056
orientation: inline

TapZ
type: ComponentDefinition
persistent identity: BBa K845005
display id: BBa_K845005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TapZ engineered membrane receptor
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K845006
component: BBa_K845007
sequence annotation: BBa_K845006
sequence annotation: BBa_K845007
sequence: https://synbiohub.org/public/igem/BBa_K845005_sequence/1

BBa_K845006
type: Component
persistent identity: component2204298
display id: component2204298
version: 1
top level: TapZ
access: public
definition: BBa_K845006

BBa_K845007
type: Component
persistent identity: component2204300
display id: component2204300
version: 1
top level: TapZ
access: public
definition: BBa_K845007

BBa_K845006
type: SequenceAnnotation
persistent identity: annotation2204298
display id: annotation2204298
version: 1
top level: TapZ
location: https://synbiohub.org/public/igem/BBa_K845005/annotation2204298/range2204298/1
component: BBa_K845006

https://synbiohub.org/public/igem/BBa_K845005/annotation2204298/range2204298/1
type: Range
persistent identity: range2204298
display id: range2204298
version: 1
direction: direction/reverse
top level: TapZ
start: 1
end: 765
orientation: inline

BBa_K845007
type: SequenceAnnotation
persistent identity: annotation2204300
display id: annotation2204300
version: 1
top level: TapZ
location: https://synbiohub.org/public/igem/BBa_K845005/annotation2204300/range2204300/1
component: BBa_K845007

https://synbiohub.org/public/igem/BBa_K845005/annotation2204300/range2204300/1
type: Range
persistent identity: range2204300
display id: range2204300
version: 1
direction: direction/reverse
top level: TapZ
start: 766
end: 1449
orientation: inline