//cds/membrane/extracellular
type: Collection
persistent identity: cds membrane extracellular
display id: cds_membrane_extracellular
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K533003
member: prhA
member: BBa_K533005
member: BBa_K258006
member: BBa_K618212
member: BBa_K618112
member: BBa_K618211
member: BBa_K1404006
member: BBa_K1404008
member: BBa_K133061
member: BBa_K2168000
member: BBa_K533004
member: BBa_K808030
member: BBa_K618111
member: BBa_K808027
member: BBa_K1404001
member: BBa_K1404007
member: PrtDEF
member: BBa_K2168001

BBa_K133061
type: ComponentDefinition
persistent identity: BBa K133061
display id: BBa_K133061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4ecto
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Extracellular domain of T-cell surface glycoprotein CD4.
mutable provenance: Obtained from biobrick part BBa_I712084.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133061_sequence/1

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CsgG signal
sequence annotation: Sec signal
sequence annotation: Beta sheet4
sequence annotation: RBS Paris 2009
sequence annotation: mutation
sequence annotation: Beta sheet5
sequence annotation: Beta sheet1
sequence annotation: Beta sheet3
sequence annotation: CsgA-WT
sequence annotation: Beta sheet2
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

BBa_K618212
type: ComponentDefinition
persistent identity: BBa K618212
display id: BBa_K618212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLAC ompA-zif268
creator: Ben Park, Chris Bate, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein zif268 under the control of pLAC. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165006 is supplied on a Silver lab vector (Bba_J63010) with a modified biobrick prefix, the scar between ompA and zif268 is non-standard. The G at position 700 is not there. This has been verified by sequencing. Deleting the G keeps zif268 in frame with ompA. Part length is 1091bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K165006
component: BBa_K103006
sequence annotation: BBa_K103006
sequence annotation: BBa_J04500
sequence annotation: BBa_K165006
sequence: https://synbiohub.org/public/igem/BBa_K618212_sequence/1

BBa_J04500
type: Component
persistent identity: component2144992
display id: component2144992
version: 1
top level: BBa_K618212
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K165006
type: Component
persistent identity: component2144999
display id: component2144999
version: 1
top level: BBa_K618212
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K103006
type: Component
persistent identity: component2144998
display id: component2144998
version: 1
top level: BBa_K618212
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144998
display id: annotation2144998
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144998/range2144998/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618212/annotation2144998/range2144998/1
type: Range
persistent identity: range2144998
display id: range2144998
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 229
end: 692
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2144992
display id: annotation2144992
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144992/range2144992/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K618212/annotation2144992/range2144992/1
type: Range
persistent identity: range2144992
display id: range2144992
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 1
end: 220
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2144999
display id: annotation2144999
version: 1
top level: BBa_K618212
location: https://synbiohub.org/public/igem/BBa_K618212/annotation2144999/range2144999/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K618212/annotation2144999/range2144999/1
type: Range
persistent identity: range2144999
display id: range2144999
version: 1
direction: direction/reverse
top level: BBa_K618212
start: 701
end: 952
orientation: inline

BBa_K533005
type: ComponentDefinition
persistent identity: BBa K533005
display id: BBa_K533005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-GFP
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP is presented outside E. Coli as a positive control for protein presentation
mutable notes: His tag is inserted between OmpA and GFP for easier detection in Western Blotting.
mutable provenance: OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rbs
sequence annotation: stop
sequence annotation: GFP
sequence annotation: start
sequence annotation: His Tag
sequence annotation: lacI
sequence annotation: T7
sequence annotation: OmpA
sequence: https://synbiohub.org/public/igem/BBa_K533005_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2150599
display id: annotation2150599
version: 1
top level: BBa_K533005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150599/range2150599/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150599/range2150599/1
type: Range
persistent identity: range2150599
display id: range2150599
version: 1
direction: direction/forward
top level: BBa_K533005
start: 75
end: 79
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2150603
display id: annotation2150603
version: 1
top level: BBa_K533005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150603/range2150603/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150603/range2150603/1
type: Range
persistent identity: range2150603
display id: range2150603
version: 1
direction: direction/forward
top level: BBa_K533005
start: 1416
end: 1418
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2150598
display id: annotation2150598
version: 1
top level: BBa_K533005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150598/range2150598/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150598/range2150598/1
type: Range
persistent identity: range2150598
display id: range2150598
version: 1
direction: direction/forward
top level: BBa_K533005
start: 696
end: 1418
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2150604
display id: annotation2150604
version: 1
top level: BBa_K533005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150604/range2150604/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150604/range2150604/1
type: Range
persistent identity: range2150604
display id: range2150604
version: 1
direction: direction/forward
top level: BBa_K533005
start: 87
end: 89
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2150600
display id: annotation2150600
version: 1
top level: BBa_K533005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150600/range2150600/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150600/range2150600/1
type: Range
persistent identity: range2150600
display id: range2150600
version: 1
direction: direction/forward
top level: BBa_K533005
start: 648
end: 665
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150601
display id: annotation2150601
version: 1
top level: BBa_K533005
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150601/range2150601/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150601/range2150601/1
type: Range
persistent identity: range2150601
display id: range2150601
version: 1
direction: direction/forward
top level: BBa_K533005
start: 20
end: 44
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150602
display id: annotation2150602
version: 1
top level: BBa_K533005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150602/range2150602/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150602/range2150602/1
type: Range
persistent identity: range2150602
display id: range2150602
version: 1
direction: direction/forward
top level: BBa_K533005
start: 1
end: 18
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150597
display id: annotation2150597
version: 1
top level: BBa_K533005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150597/range2150597/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150597/range2150597/1
type: Range
persistent identity: range2150597
display id: range2150597
version: 1
direction: direction/forward
top level: BBa_K533005
start: 87
end: 633
orientation: inline

BBa_K808027
type: ComponentDefinition
persistent identity: BBa K808027
display id: BBa_K808027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EstA : inactive fusion protein for bacterial surface expression
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Esterase A is a inactivated membrane bound esterase from Pseudomonas putida. Its active site is mutated so it shows no more hydrolytic activity. It can be used for cell surface display like its shown in the following paper: Taek Ho Yang, Jae Gu Pan, Yeon Soo Seo, Joon Shick Rhee Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli Appl Environ Microbiol. 2004 December; 70(12): 6968???6976. doi: 10.1128/AEM.70.12.6968-6976.2004
mutable notes: This certain variant of EstA should be used for cell surface expression and displaying of almost every kind of enzyme. There is no ATG at its 5' because its an C-terminal fusion protein. If you want to use you need to design it in the following way: N - Peri plasmatic signal sequence - Protein of interest - EstA - C We did not use any kind of BioBrick Assembly due to the generation of stop codons. If you would like to fuse EstA to any kind of protein, please use SOE PCR or an alternative kind of assembly strategy.
mutable provenance: Our parts derives from an established inactive EstA mutant on pEST100 vector, which was used in the following paper: Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808027_sequence/1

prhA
type: ComponentDefinition
persistent identity: BBa K367000
display id: BBa_K367000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: prhA coding sequence
creator: Fernando Govantes
created: 2010-06-28T11:00:00Z
modified: 2015-05-08T01:12:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _492_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Ralstonia solanacearum prhA gene, encoding an outer membrane protein that senses a signal on plant cell walls and transduces it through the bacterial cell envelop to stimulate transcription from several operons
mutable notes: Not yet edited, other than changing stop codon to TAATAA
mutable provenance: Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence NCBI Reference Sequence: NC_003296.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: prhA standard biobrick double stop codon
sequence annotation: prhA start codon
sequence annotation: prhA coding region
sequence: https://synbiohub.org/public/igem/BBa_K367000_sequence/1

prhA standard biobrick double stop codon
type: SequenceAnnotation
persistent identity: annotation2072036
display id: annotation2072036
version: 1
top level: prhA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K367000/annotation2072036/range2072036/1

https://synbiohub.org/public/igem/BBa_K367000/annotation2072036/range2072036/1
type: Range
persistent identity: range2072036
display id: range2072036
version: 1
direction: direction/forward
top level: prhA
start: 2311
end: 2316
orientation: inline

prhA start codon
type: SequenceAnnotation
persistent identity: annotation2072035
display id: annotation2072035
version: 1
top level: prhA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K367000/annotation2072035/range2072035/1

https://synbiohub.org/public/igem/BBa_K367000/annotation2072035/range2072035/1
type: Range
persistent identity: range2072035
display id: range2072035
version: 1
direction: direction/forward
top level: prhA
start: 1
end: 3
orientation: inline

prhA coding region
type: SequenceAnnotation
persistent identity: annotation2072031
display id: annotation2072031
version: 1
top level: prhA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K367000/annotation2072031/range2072031/1

https://synbiohub.org/public/igem/BBa_K367000/annotation2072031/range2072031/1
type: Range
persistent identity: range2072031
display id: range2072031
version: 1
direction: direction/forward
top level: prhA
start: 1
end: 2310
orientation: inline

BBa_K618112
type: ComponentDefinition
persistent identity: BBa K618112
display id: BBa_K618112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD ompA-zif268
creator: Chris Bate, Ben Park, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein zif268 under the control of pBAD. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165006 is supplied on a Silver lab vector (Bba_J63010) with a modified biobrick prefix, the scar between ompA and zif268 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps zif268 in frame with ompA. Part length is 1364bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K259007
component: BBa_K103006
component: BBa_K165006
sequence annotation: BBa_K103006
sequence annotation: BBa_K259007
sequence annotation: BBa_K165006
sequence: https://synbiohub.org/public/igem/BBa_K618112_sequence/1

BBa_K259007
type: Component
persistent identity: component2144949
display id: component2144949
version: 1
top level: BBa_K618112
access: public
definition: https://synbiohub.org/public/igem/BBa_K259007/1

BBa_K103006
type: Component
persistent identity: component2144955
display id: component2144955
version: 1
top level: BBa_K618112
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K165006
type: Component
persistent identity: component2144956
display id: component2144956
version: 1
top level: BBa_K618112
access: public
definition: https://synbiohub.org/public/igem/BBa_K165006/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144955
display id: annotation2144955
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144955/range2144955/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K618112/annotation2144955/range2144955/1
type: Range
persistent identity: range2144955
display id: range2144955
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 369
end: 832
orientation: inline

BBa_K259007
type: SequenceAnnotation
persistent identity: annotation2144949
display id: annotation2144949
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144949/range2144949/1
component: BBa_K259007

https://synbiohub.org/public/igem/BBa_K618112/annotation2144949/range2144949/1
type: Range
persistent identity: range2144949
display id: range2144949
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 1
end: 360
orientation: inline

BBa_K165006
type: SequenceAnnotation
persistent identity: annotation2144956
display id: annotation2144956
version: 1
top level: BBa_K618112
location: https://synbiohub.org/public/igem/BBa_K618112/annotation2144956/range2144956/1
component: BBa_K165006

https://synbiohub.org/public/igem/BBa_K618112/annotation2144956/range2144956/1
type: Range
persistent identity: range2144956
display id: range2144956
version: 1
direction: direction/reverse
top level: BBa_K618112
start: 841
end: 1092
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: His tag
sequence annotation: CsgD signal
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet5
sequence annotation: Beta sheet
sequence annotation: Beta sheet2
sequence annotation: Linker
sequence annotation: CsgA-His2
sequence annotation: BBa_K342000
sequence annotation: Beta sheet4
sequence annotation: GCT into GCC
sequence annotation: linker
sequence annotation: SEC signal
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

BBa_K2168001
type: ComponentDefinition
persistent identity: BBa K2168001
display id: BBa_K2168001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - (N-6xHis) - Ag43 - sfGFP
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T03:15:53Z
discontinued: false
dominant: false
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a fusion protein with Ag43, a protein from E coli tagged with a 6xHis tag on the N-terminal and sfGFP tagged on C-terminal of the pro-protein. Ag43 causes cell-cell adhesion in E coli, and the 6xHis and sfGFP tags are present to allow convenient study dependence of Ag43 mediated aggregation on Ag43 expression levels, which can be determined from the sfGFP fluoresence and/or quantification of 6xHis tag. The 6xHis tag is included to allow protein concentration estimation in case the sfGFP fails to fold properly.
mutable notes: The location for the 6xHis tag is the same as that for BBa_K2168000(Ag43 tagged with 6xHis on N-terminal under pBAD/araC promoter) and uses the same design principles. For independent folding, a flexible GGGGSGGGGS linker was added between Ag43 and sfGFP, and the linker was choosen to be not very hydrophilic or hydrophobic, since Ag43 lies very close to lipid membranes.
mutable provenance: This part was made from Gibson assemblies from fragments of two previous parts - BBa_K1352000 and BBa_I746908, which code for Ag43 and sfGFP respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: (GGGGS)2 linker
sequence annotation: Double terminator (B0015)
sequence annotation: 6xHis
sequence annotation: sfGFP
sequence annotation: B0034
sequence annotation: pBAD
sequence annotation: Ag43(PstI sites removed)
sequence annotation: araC
sequence: https://synbiohub.org/public/igem/BBa_K2168001_sequence/1

(GGGGS)2 linker
type: SequenceAnnotation
persistent identity: annotation2499487
display id: annotation2499487
version: 1
top level: BBa_K2168001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1
type: Range
persistent identity: range2499487
display id: range2499487
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4372
end: 4401
orientation: inline

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499489
display id: annotation2499489
version: 1
top level: BBa_K2168001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1
type: Range
persistent identity: range2499489
display id: range2499489
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 5130
end: 5258
orientation: inline

6xHis
type: SequenceAnnotation
persistent identity: annotation2499490
display id: annotation2499490
version: 1
top level: BBa_K2168001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1
type: Range
persistent identity: range2499490
display id: range2499490
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1396
end: 1413
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2499488
display id: annotation2499488
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1
type: Range
persistent identity: range2499488
display id: range2499488
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4402
end: 5118
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499485
display id: annotation2499485
version: 1
top level: BBa_K2168001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1
type: Range
persistent identity: range2499485
display id: range2499485
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1219
end: 1230
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499484
display id: annotation2499484
version: 1
top level: BBa_K2168001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1
type: Range
persistent identity: range2499484
display id: range2499484
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 906
end: 1190
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499486
display id: annotation2499486
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1
type: Range
persistent identity: range2499486
display id: range2499486
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1237
end: 4371
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499465
display id: annotation2499465
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1
type: Range
persistent identity: range2499465
display id: range2499465
version: 1
direction: direction/reverse
top level: BBa_K2168001
start: 1
end: 879
orientation: inline

BBa_K808030
type: ComponentDefinition
persistent identity: BBa K808030
display id: BBa_K808030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
mutable notes: We got it synthesized by GENEART but added the RBS via PCR.
mutable provenance: This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K808026
sequence annotation: BBa_K808027
sequence annotation: myctag
sequence annotation: BBa_B0029
sequence annotation: BBa_K808028
sequence annotation: stop
sequence annotation: start
sequence annotation: 6 histag
sequence: https://synbiohub.org/public/igem/BBa_K808030_sequence/1

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2182935
display id: annotation2182935
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1
type: Range
persistent identity: range2182935
display id: range2182935
version: 1
direction: direction/forward
top level: BBa_K808030
start: 104
end: 1573
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2182937
display id: annotation2182937
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1
type: Range
persistent identity: range2182937
display id: range2182937
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1604
end: 3037
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2182936
display id: annotation2182936
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1
type: Range
persistent identity: range2182936
display id: range2182936
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1574
end: 1603
orientation: inline

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2182926
display id: annotation2182926
version: 1
top level: BBa_K808030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1
type: Range
persistent identity: range2182926
display id: range2182926
version: 1
direction: direction/forward
top level: BBa_K808030
start: 2
end: 16
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2182934
display id: annotation2182934
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1
type: Range
persistent identity: range2182934
display id: range2182934
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 85
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182939
display id: annotation2182939
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1
type: Range
persistent identity: range2182939
display id: range2182939
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3041
end: 3043
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2182940
display id: annotation2182940
version: 1
top level: BBa_K808030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1
type: Range
persistent identity: range2182940
display id: range2182940
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 25
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182938
display id: annotation2182938
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1
type: Range
persistent identity: range2182938
display id: range2182938
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3038
end: 3040
orientation: inline

6 histag
type: SequenceAnnotation
persistent identity: annotation2182933
display id: annotation2182933
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1
type: Range
persistent identity: range2182933
display id: range2182933
version: 1
direction: direction/forward
top level: BBa_K808030
start: 86
end: 103
orientation: inline

BBa_K533004
type: ComponentDefinition
persistent identity: BBa K533004
display id: BBa_K533004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-mCherry
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is designed as the positive control for protein presentation on the outer membrane.
mutable notes: His tag is inserted between OmpA and mCherry to facilitate detection after expression.
mutable provenance: OmpA is cloned from genomic DNA of BL21(DE3) E. Coli strain. mCherry is from the Clontech plasmid pmCherry-C1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: mCherry
sequence annotation: T7
sequence annotation: stop
sequence annotation: OmpA
sequence annotation: ATG
sequence annotation: His6Tag
sequence annotation: lacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K533004_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2150611
display id: annotation2150611
version: 1
top level: BBa_K533004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150611/range2150611/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150611/range2150611/1
type: Range
persistent identity: range2150611
display id: range2150611
version: 1
direction: direction/forward
top level: BBa_K533004
start: 699
end: 1409
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150605
display id: annotation2150605
version: 1
top level: BBa_K533004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150605/range2150605/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150605/range2150605/1
type: Range
persistent identity: range2150605
display id: range2150605
version: 1
direction: direction/forward
top level: BBa_K533004
start: 1
end: 18
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2150612
display id: annotation2150612
version: 1
top level: BBa_K533004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150612/range2150612/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150612/range2150612/1
type: Range
persistent identity: range2150612
display id: range2150612
version: 1
direction: direction/forward
top level: BBa_K533004
start: 1407
end: 1409
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150609
display id: annotation2150609
version: 1
top level: BBa_K533004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150609/range2150609/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150609/range2150609/1
type: Range
persistent identity: range2150609
display id: range2150609
version: 1
direction: direction/forward
top level: BBa_K533004
start: 87
end: 633
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150608
display id: annotation2150608
version: 1
top level: BBa_K533004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150608/range2150608/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150608/range2150608/1
type: Range
persistent identity: range2150608
display id: range2150608
version: 1
direction: direction/forward
top level: BBa_K533004
start: 87
end: 89
orientation: inline

His6Tag
type: SequenceAnnotation
persistent identity: annotation2150610
display id: annotation2150610
version: 1
top level: BBa_K533004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150610/range2150610/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150610/range2150610/1
type: Range
persistent identity: range2150610
display id: range2150610
version: 1
direction: direction/forward
top level: BBa_K533004
start: 648
end: 665
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150606
display id: annotation2150606
version: 1
top level: BBa_K533004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150606/range2150606/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150606/range2150606/1
type: Range
persistent identity: range2150606
display id: range2150606
version: 1
direction: direction/forward
top level: BBa_K533004
start: 20
end: 44
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150607
display id: annotation2150607
version: 1
top level: BBa_K533004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150607/range2150607/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150607/range2150607/1
type: Range
persistent identity: range2150607
display id: range2150607
version: 1
direction: direction/forward
top level: BBa_K533004
start: 75
end: 79
orientation: inline

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet 1
sequence annotation: Beta sheet5
sequence annotation: Beta sheet3
sequence annotation: CsgG signal
sequence annotation: Linker
sequence annotation: GCT into GCC
sequence annotation: BBa_K342000
sequence annotation: His tag
sequence annotation: CsgA-His1
sequence annotation: Beta sheet4
sequence annotation: Beta sheet2
sequence annotation: Sec signal
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

BBa_K258006
type: ComponentDefinition
persistent identity: BBa K258006
display id: BBa_K258006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi.
mutable notes: The sequence was optimized for E.coli with Biobrick restriction sites
mutable provenance: The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258006_sequence/1

BBa_K2168000
type: ComponentDefinition
persistent identity: BBa K2168000
display id: BBa_K2168000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - N(6xHis) Ag43
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T02:44:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.
mutable notes: Ag43 is an outer membrane E coli protein, so it has a N-terminal 52 amino acid long signal peptide sequence, which is cleaved after Ag43 pro-protein is transported into the periplasmic space. Since strong dependence has been demonstrated on the signal peptide cleavage on the signal peptide-protein junction, the 6xHis tag should be added after Ala53, the residue immediately after the signal peptide sequence.
mutable provenance: This comes from Gibson assembly of fragments from a previous biobrick, BBa_K1352000 by Team:Aberdeen_Scotland, 2014.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6xHis tag
sequence annotation: Double terminator (B0015)
sequence annotation: pBAD
sequence annotation: Ag43(PstI sites removed)
sequence annotation: B0034
sequence annotation: araC
sequence: https://synbiohub.org/public/igem/BBa_K2168000_sequence/1

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2499423
display id: annotation2499423
version: 1
top level: BBa_K2168000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1
type: Range
persistent identity: range2499423
display id: range2499423
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1396
end: 1413
orientation: inline

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499426
display id: annotation2499426
version: 1
top level: BBa_K2168000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1
type: Range
persistent identity: range2499426
display id: range2499426
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 4383
end: 4511
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499411
display id: annotation2499411
version: 1
top level: BBa_K2168000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1
type: Range
persistent identity: range2499411
display id: range2499411
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 906
end: 1190
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499418
display id: annotation2499418
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1
type: Range
persistent identity: range2499418
display id: range2499418
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1237
end: 4374
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499416
display id: annotation2499416
version: 1
top level: BBa_K2168000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1
type: Range
persistent identity: range2499416
display id: range2499416
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1219
end: 1230
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499395
display id: annotation2499395
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1
type: Range
persistent identity: range2499395
display id: range2499395
version: 1
direction: direction/reverse
top level: BBa_K2168000
start: 1
end: 879
orientation: inline

BBa_K618211
type: ComponentDefinition
persistent identity: BBa K618211
display id: BBa_K618211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLAC ompA-Gli1
creator: Ben Park, Chris Bate, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein Gli-1 under the control of pLAC. The protein should be present on the outer surface of cells and bind the Gli-1 recognition sequence (GACCACCCAAGACGA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.
mutable notes: NOTE- the sequence as listed here is wrong. Because part K165007 is supplied on a Silver lab vector (Bba_J63009 or V0002) with a modified biobrick prefix, the scar between ompA and Gli-1 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps Gli-1 in frame with ompA. Part length is 1364bp. The sequence was generated automatically and I couldn't figure out how to edit it.
mutable provenance: parts come from iGEM distribution and from iGEM HQ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K103006
component: BBa_J04500
component: BBa_K165007
sequence annotation: BBa_J04500
sequence annotation: BBa_K165007
sequence annotation: BBa_K103006
sequence: https://synbiohub.org/public/igem/BBa_K618211_sequence/1

BBa_K103006
type: Component
persistent identity: component2144977
display id: component2144977
version: 1
top level: BBa_K618211
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_J04500
type: Component
persistent identity: component2144971
display id: component2144971
version: 1
top level: BBa_K618211
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K165007
type: Component
persistent identity: component2144978
display id: component2144978
version: 1
top level: BBa_K618211
access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2144971
display id: annotation2144971
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144971/range2144971/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K618211/annotation2144971/range2144971/1
type: Range
persistent identity: range2144971
display id: range2144971
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 1
end: 220
orientation: inline

BBa_K165007
type: SequenceAnnotation
persistent identity: annotation2144978
display id: annotation2144978
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144978/range2144978/1
component: BBa_K165007

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type: Range
persistent identity: range2144978
display id: range2144978
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 701
end: 1225
orientation: inline

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144977
display id: annotation2144977
version: 1
top level: BBa_K618211
location: https://synbiohub.org/public/igem/BBa_K618211/annotation2144977/range2144977/1
component: BBa_K103006

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type: Range
persistent identity: range2144977
display id: range2144977
version: 1
direction: direction/reverse
top level: BBa_K618211
start: 229
end: 692
orientation: inline

BBa_K533003
type: ComponentDefinition
persistent identity: BBa K533003
display id: BBa_K533003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-HIV aspartyl protease
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
mutable notes: Since HIV protease is a dimer, dimerization is a great concern to its function. Function of this protease is tested in BL21.
mutable provenance: HIV protease sequence is from former part BBa_I712667. OmpA sequence is from BL21(DE3) e. Coli strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: OmpA
sequence annotation: T7
sequence annotation: lacI
sequence annotation: START
sequence annotation: STOP
sequence annotation: HIV protease
sequence annotation: BBa_I712667
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K533003_sequence/1

OmpA
type: SequenceAnnotation
persistent identity: annotation2150441
display id: annotation2150441
version: 1
top level: BBa_K533003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150441/range2150441/1

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type: Range
persistent identity: range2150441
display id: range2150441
version: 1
direction: direction/forward
top level: BBa_K533003
start: 87
end: 632
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150439
display id: annotation2150439
version: 1
top level: BBa_K533003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1
type: Range
persistent identity: range2150439
display id: range2150439
version: 1
direction: direction/forward
top level: BBa_K533003
start: 1
end: 18
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150440
display id: annotation2150440
version: 1
top level: BBa_K533003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150440/range2150440/1

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type: Range
persistent identity: range2150440
display id: range2150440
version: 1
direction: direction/forward
top level: BBa_K533003
start: 20
end: 44
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2146916
display id: annotation2146916
version: 1
top level: BBa_K533003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1
type: Range
persistent identity: range2146916
display id: range2146916
version: 1
direction: direction/forward
top level: BBa_K533003
start: 87
end: 89
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2146917
display id: annotation2146917
version: 1
top level: BBa_K533003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146917/range2146917/1

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type: Range
persistent identity: range2146917
display id: range2146917
version: 1
direction: direction/forward
top level: BBa_K533003
start: 933
end: 935
orientation: inline

HIV protease
type: SequenceAnnotation
persistent identity: annotation2146915
display id: annotation2146915
version: 1
top level: BBa_K533003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1
type: Range
persistent identity: range2146915
display id: range2146915
version: 1
direction: direction/forward
top level: BBa_K533003
start: 633
end: 935
orientation: inline

BBa_I712667
type: SequenceAnnotation
persistent identity: annotation2146918
display id: annotation2146918
version: 1
top level: BBa_K533003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1
type: Range
persistent identity: range2146918
display id: range2146918
version: 1
direction: direction/reverse
top level: BBa_K533003
start: 633
end: 935
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150442
display id: annotation2150442
version: 1
top level: BBa_K533003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1
type: Range
persistent identity: range2150442
display id: range2150442
version: 1
direction: direction/forward
top level: BBa_K533003
start: 75
end: 79
orientation: inline

PrtDEF
type: ComponentDefinition
persistent identity: BBa K258008
display id: BBa_K258008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37??C, the optimum growth temperature for E. coli
mutable notes: We used whole plasmid in order to excrete a fusion protein with one of two signal tags which are TliA and LARD1
mutable provenance: Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0010
component: BBa_B0034
component: BBa_K258102
component: BBa_R0040
component: BBa_K258100
component: BBa_K258101
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K258100
sequence annotation: BBa_B0012
sequence annotation: BBa_K258102
sequence annotation: BBa_K258101
sequence: https://synbiohub.org/public/igem/BBa_K258008_sequence/1

BBa_B0010
type: Component
persistent identity: component2033405
display id: component2033405
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2033398
display id: component2033398
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K258102
type: Component
persistent identity: component2033404
display id: component2033404
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258102/1

BBa_R0040
type: Component
persistent identity: component2033387
display id: component2033387
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component2033400
display id: component2033400
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2033403
display id: component2033403
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K258100
type: Component
persistent identity: component2033394
display id: component2033394
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258100/1

BBa_K258101
type: Component
persistent identity: component2033399
display id: component2033399
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_K258101/1

BBa_B0034
type: Component
persistent identity: component2033393
display id: component2033393
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2033395
display id: component2033395
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2033407
display id: component2033407
version: 1
top level: PrtDEF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2033387
display id: annotation2033387
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K258008/annotation2033387/range2033387/1
type: Range
persistent identity: range2033387
display id: range2033387
version: 1
direction: direction/forward
top level: PrtDEF
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033400
display id: annotation2033400
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033400/range2033400/1
component: BBa_B0010

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type: Range
persistent identity: range2033400
display id: range2033400
version: 1
direction: direction/forward
top level: PrtDEF
start: 3272
end: 3351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033393
display id: annotation2033393
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033393/range2033393/1
component: BBa_B0034

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type: Range
persistent identity: range2033393
display id: range2033393
version: 1
direction: direction/reverse
top level: PrtDEF
start: 63
end: 74
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033403
display id: annotation2033403
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K258008/annotation2033403/range2033403/1
type: Range
persistent identity: range2033403
display id: range2033403
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3360
end: 3371
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033405
display id: annotation2033405
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033405/range2033405/1
component: BBa_B0010

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type: Range
persistent identity: range2033405
display id: range2033405
version: 1
direction: direction/forward
top level: PrtDEF
start: 4772
end: 4851
orientation: inline

BBa_K258100
type: SequenceAnnotation
persistent identity: annotation2033394
display id: annotation2033394
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
component: BBa_K258100

https://synbiohub.org/public/igem/BBa_K258008/annotation2033394/range2033394/1
type: Range
persistent identity: range2033394
display id: range2033394
version: 1
direction: direction/reverse
top level: PrtDEF
start: 81
end: 1805
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2033407
display id: annotation2033407
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033407/range2033407/1
component: BBa_B0012

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type: Range
persistent identity: range2033407
display id: range2033407
version: 1
direction: direction/forward
top level: PrtDEF
start: 4860
end: 4900
orientation: inline

BBa_K258102
type: SequenceAnnotation
persistent identity: annotation2033404
display id: annotation2033404
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033404/range2033404/1
component: BBa_K258102

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type: Range
persistent identity: range2033404
display id: range2033404
version: 1
direction: direction/reverse
top level: PrtDEF
start: 3378
end: 4763
orientation: inline

BBa_K258101
type: SequenceAnnotation
persistent identity: annotation2033399
display id: annotation2033399
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033399/range2033399/1
component: BBa_K258101

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type: Range
persistent identity: range2033399
display id: range2033399
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1920
end: 3263
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2033398
display id: annotation2033398
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033398/range2033398/1
component: BBa_B0034

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type: Range
persistent identity: range2033398
display id: range2033398
version: 1
direction: direction/reverse
top level: PrtDEF
start: 1902
end: 1913
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2033395
display id: annotation2033395
version: 1
top level: PrtDEF
location: https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K258008/annotation2033395/range2033395/1
type: Range
persistent identity: range2033395
display id: range2033395
version: 1
direction: direction/forward
top level: PrtDEF
start: 1814
end: 1893
orientation: inline

BBa_K1404001
type: ComponentDefinition
persistent identity: BBa K1404001
display id: BBa_K1404001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part, when expressed in a rcnA E. coli strain, allows the formation of a functional Ni2+ and Co2+ biofilter capturing up to 3 mg of metal per g of bacterial dry weight when exposed to 20 ??M Ni2+ or Co2+, in only 10 mn. This is one of the best performances and the fastest reported until now.
mutable notes: Temp
mutable provenance: [lien] and [lien] were cloned by 3A assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K540000
component: BBa_K1404002
sequence annotation: BBa_K540000
sequence annotation: BBa_K1404002
sequence: https://synbiohub.org/public/igem/BBa_K1404001_sequence/1

BBa_K540000
type: Component
persistent identity: component2385428
display id: component2385428
version: 1
top level: BBa_K1404001
access: public
definition: https://synbiohub.org/public/igem/BBa_K540000/1

BBa_K1404002
type: Component
persistent identity: component2385413
display id: component2385413
version: 1
top level: BBa_K1404001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1404002/1

BBa_K540000
type: SequenceAnnotation
persistent identity: annotation2385428
display id: annotation2385428
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385428/range2385428/1
component: BBa_K540000

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type: Range
persistent identity: range2385428
display id: range2385428
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1315
end: 4437
orientation: inline

BBa_K1404002
type: SequenceAnnotation
persistent identity: annotation2385413
display id: annotation2385413
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
component: BBa_K1404002

https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
type: Range
persistent identity: range2385413
display id: range2385413
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1
end: 1306
orientation: inline

BBa_K618111
type: ComponentDefinition
persistent identity: BBa K618111
display id: BBa_K618111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD ompA-Gli1
creator: Chris Bate, Ben Park, Marc Ammerlaan
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _790_
m user id: 0
owner id: 8977
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a fusion between ompA and the DNA binding protein Gli-1. It should be present on the outer surface of cells and bind the Gli-! recognition sequence there. This will also allows cells to be anchored to glass slide with the DNA attached.
mutable notes: Silver prefix on Gli-1 allowed us to fuse it to ompA in frame.
mutable provenance: parts come from iGEM distribution
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K165007
component: BBa_K259007
component: BBa_K103006
sequence annotation: BBa_K103006
sequence annotation: BBa_K259007
sequence annotation: BBa_K165007
sequence: https://synbiohub.org/public/igem/BBa_K618111_sequence/1

BBa_K165007
type: Component
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access: public
definition: https://synbiohub.org/public/igem/BBa_K165007/1

BBa_K259007
type: Component
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display id: component2144556
version: 1
top level: BBa_K618111
access: public
definition: https://synbiohub.org/public/igem/BBa_K259007/1

BBa_K103006
type: Component
persistent identity: component2144562
display id: component2144562
version: 1
top level: BBa_K618111
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation2144562
display id: annotation2144562
version: 1
top level: BBa_K618111
location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144562/range2144562/1
component: BBa_K103006

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BBa_K259007
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location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144556/range2144556/1
component: BBa_K259007

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persistent identity: range2144556
display id: range2144556
version: 1
direction: direction/reverse
top level: BBa_K618111
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end: 360
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BBa_K165007
type: SequenceAnnotation
persistent identity: annotation2144563
display id: annotation2144563
version: 1
top level: BBa_K618111
location: https://synbiohub.org/public/igem/BBa_K618111/annotation2144563/range2144563/1
component: BBa_K165007

https://synbiohub.org/public/igem/BBa_K618111/annotation2144563/range2144563/1
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display id: range2144563
version: 1
direction: direction/reverse
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start: 841
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orientation: inline