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//cds/membrane
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//cds/membrane/pump
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//cds/membrane/channel
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//cds/membrane/extracellular
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//cds/membrane/transporter
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BBa_K103016
type: ComponentDefinition
persistent identity: BBa K103016
display id: BBa_K103016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA_linker_omega_linker
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
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sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
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type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K103006
component: BBa_K103012
sequence annotation: BBa_K103012
sequence annotation: BBa_K103006
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Switch
type: ComponentDefinition
persistent identity: BBa K1185000
display id: BBa_K1185000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B.subtilis Rod to L-form Switch
creator: Vincent Leonardo, Christopher Wall and Robert Mckiernan
created: 2013-08-14T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
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sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick facilitates the switching of Bacillus subtilis cells between rod-shape to L-form, a cell wall-less form of bacteria. It consists of chloramphenicol acetyl-transferase (cat) gene and a xylose-controlled promoter (PxylR) which are flanked by ~300bp pbpB gene at the 5???end and ~300bp of murE gene at 3???end. These flanking sequences allows the integration of the BioBrick into the chromosome by homologous recombination replacing the region containing the end of pbpb gene and the beginning of the murE gene, which are involved in peptidoglycan biosynthesis. This event replaces the constituitive murE promoter with a PxylR upstream of the murE gene. Therefore, when xylose is present cell wall biosynthesis is switched on, giving rod cells. When xylose is not present, the cells will become L-forms. Simply transform the BioBrick into competent B. subtilis cells and through recombination it will integrate into cells chromosome. Plate out the transformants onto 1.5% lysogeny broth agar containing 5??g/ml Chloramphenicol and 0.8 %( w/v) xylose. This will give you rod cells containing biobrick. To get L-forms, inoculate some colonies into a solution containing an equal amount of nutrient broth and MSM. Also inoculate some transformants into the same media with the addition of 0.8 %( w/v) xylose to clarify that the switch BioBrick is working. Use microscopy to check the morphology of the cells in each media. L-forms will appear near spherical and can vary in size considerably.
mutable notes: There were 3 restriction sites that did not meet the RFC10 requirements, two EcoRI sites and one PstI. The first EcoRI restriction site was located 218bp into the whole BioBrick (inside pbpb region), we changed the sequence from GAATTC to GCATTC. The second restriction site was located 2287bp into the BioBrick in the spacer region between PxylR and murE, we changed it from GAATTC to GTATTC. The PstI site was located 197bp into the BioBrick in the pbpb region and was change from CTGCAG to CTTCAG.
mutable provenance: The part is the courtesy of Prof. Jeff Errington research group, and all the sequences come from the Bacillus subtilis genomic sequence. The pbpb section in the BioBrick was taken from end of the pbpb gene 1841-2151bp (309bp). Sequence acquired through microbes online VIMSS38051. The murE section was taken from the start of the murE gene 1-331bp. Sequence from microbes online VIMSS38053. The cat gene and PxylR gene were both come from the B. subtilis genome, but can also be found in the BioBrick Registry (BBa_K823020 and BBa_K143014).
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type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: xylose-controlled promoter (PxylR)
sequence annotation: murE RBS with spacers
sequence annotation: pbpb Homologous region
sequence annotation: murE Homologous region
sequence annotation: BBa_K823020
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xylose-controlled promoter (PxylR)
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pbpb Homologous region
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murE Homologous region
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display id: annotation2331841
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BBa_K1933200
type: ComponentDefinition
persistent identity: BBa K1933200
display id: BBa_K1933200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T04:57:35Z
discontinued: false
dominant: false
experience: experience/Works
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status: status/Planning
bookmark: true
mutable description: anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a single chained variable fragment(scFv) of a norovirus GII.4 strain fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
mutable notes: Silent mutation was done to remove PstI site.
mutable provenance: Promoter and RBS The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. scFv is made from fusing variable region (VH and VL chain) of a human anti-norovirus antibody with a linker peptide. We have been given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1933002
component: BBa_K1933011
component: BBa_K1933004
sequence annotation: BBa_K1933002
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933011
sequence: https://synbiohub.org/public/igem/BBa_K1933200_sequence/1

BBa_K1933002
type: Component
persistent identity: component2515638
display id: component2515638
version: 1
top level: BBa_K1933200
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933002/1

BBa_K1933011
type: Component
persistent identity: component2515631
display id: component2515631
version: 1
top level: BBa_K1933200
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933011/1

BBa_K1933004
type: Component
persistent identity: component2515627
display id: component2515627
version: 1
top level: BBa_K1933200
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933002
type: SequenceAnnotation
persistent identity: annotation2515638
display id: annotation2515638
version: 1
top level: BBa_K1933200
location: https://synbiohub.org/public/igem/BBa_K1933200/annotation2515638/range2515638/1
component: BBa_K1933002

https://synbiohub.org/public/igem/BBa_K1933200/annotation2515638/range2515638/1
type: Range
persistent identity: range2515638
display id: range2515638
version: 1
direction: direction/reverse
top level: BBa_K1933200
start: 1036
end: 1815
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2515627
display id: annotation2515627
version: 1
top level: BBa_K1933200
location: https://synbiohub.org/public/igem/BBa_K1933200/annotation2515627/range2515627/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933200/annotation2515627/range2515627/1
type: Range
persistent identity: range2515627
display id: range2515627
version: 1
direction: direction/reverse
top level: BBa_K1933200
start: 1
end: 60
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2515631
display id: annotation2515631
version: 1
top level: BBa_K1933200
location: https://synbiohub.org/public/igem/BBa_K1933200/annotation2515631/range2515631/1
component: BBa_K1933011

https://synbiohub.org/public/igem/BBa_K1933200/annotation2515631/range2515631/1
type: Range
persistent identity: range2515631
display id: range2515631
version: 1
direction: direction/reverse
top level: BBa_K1933200
start: 61
end: 1035
orientation: inline

BBa_K1666000
type: ComponentDefinition
persistent identity: BBa K1666000
display id: BBa_K1666000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-08-25T11:00:00Z
modified: 2015-08-30T07:46:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2084_
m user id: 28478
owner id: 28478
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In a process called quorum sensing, bacteria communicate with one another using secreted chemical signaling molecules termed autoinducers. As the density of the bacterial population increases, so does the concentration of secreted autoinducer molecules. When the concentration of the autoinducer reaches a critical threshold, it diffuses back into the cell and activates or represses certain target genes. An autoinducer called AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In the LuxS/AI-2 signaling system of Salmonella enterica serovar Typhimurium, AI-2 response involves genes named lsr (LuxS regulated) that encode ATP binding cassette-type transporter. And LsrA is ATP-binding protein of the ABC transporter. In our project, we set this part under a nisA promoter for the final purpose of constructing an integrated AI-2 response pathway in our engineering bacteria.
mutable notes: The lsrA gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR.We added restriction enzyme sites when designing primers,and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: It comes from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2(Gene ID: 1255601)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LsrA
sequence: https://synbiohub.org/public/igem/BBa_K1666000_sequence/1

LsrA
type: SequenceAnnotation
persistent identity: annotation2439289
display id: annotation2439289
version: 1
top level: BBa_K1666000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1

https://synbiohub.org/public/igem/BBa_K1666000/annotation2439289/range2439289/1
type: Range
persistent identity: range2439289
display id: range2439289
version: 1
direction: direction/forward
top level: BBa_K1666000
start: 1
end: 1536
orientation: inline

BBa_K1761001
type: ComponentDefinition
persistent identity: BBa K1761001
display id: BBa_K1761001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer Membrane Protein X (OmpX) with BamHI-linker and mNeonGreen
creator: Laura van Smeden
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T05:38:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2183_
m user id: 25572
owner id: 25572
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of three subparts, namely the uter membrane protein OmpX, a BamHI linker and the fluorophore mNeonGreen. OmpX is an outer membrane protein with the C- and N-termini in the intracellulair domain. To be able to use OmpX as a scaffold, a non-natural amino acid needs to be introduced. This can be done by implementing the amber stop codon TAG in one of the loops of OmpX via a mutation. With a specific tRNA an azide-functionalized amino acid can be built in, which can be used for the SPAAC click chemistry reaction with DBCO functionalized groups. The BamHI linker is a 213 bp long flexible GGSGGS linker. Using the restriction enzyme BamHI, the linker can become 45 bp shorter. mNeonGreen is a yellow-green fluorescent protein. For a fluorescene measurement, the mNeonGreen fluorophore can be excitated with a laser with a wavelenght of 480 nm and readed out at 517 nm.
mutable notes: The sequence is redesigned considering codon optimalization. Also restriction sites for biobricking and classical cloning are deleted. From the BamHI linker, the coding sequecne for GGSGGS is redesigned causing that only two BamHI restriction sites remain.
mutable provenance: OmpX is a outer membrane protein naturally occuring in wildtype E.coli. The BamHI linker is inspired by the article "Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Liners" by Toon H. Evers et all from 29 August 2006. mNeonGreen naturally occurs in the organism Branchiostoma Lanceolatum.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1761000
sequence annotation: BBa_K1761003
sequence annotation: BamHI-linker
sequence annotation: OmpX
sequence annotation: mNeonGreen
sequence: https://synbiohub.org/public/igem/BBa_K1761001_sequence/1

BBa_K1761000
type: SequenceAnnotation
persistent identity: annotation2473608
display id: annotation2473608
version: 1
top level: BBa_K1761001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1761001/annotation2473608/range2473608/1

https://synbiohub.org/public/igem/BBa_K1761001/annotation2473608/range2473608/1
type: Range
persistent identity: range2473608
display id: range2473608
version: 1
direction: direction/forward
top level: BBa_K1761001
start: 1
end: 513
orientation: inline

BBa_K1761003
type: SequenceAnnotation
persistent identity: annotation2473610
display id: annotation2473610
version: 1
top level: BBa_K1761001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1761001/annotation2473610/range2473610/1

https://synbiohub.org/public/igem/BBa_K1761001/annotation2473610/range2473610/1
type: Range
persistent identity: range2473610
display id: range2473610
version: 1
direction: direction/forward
top level: BBa_K1761001
start: 727
end: 1437
orientation: inline

BamHI-linker
type: SequenceAnnotation
persistent identity: annotation2473620
display id: annotation2473620
version: 1
top level: BBa_K1761001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1761001/annotation2473620/range2473620/1

https://synbiohub.org/public/igem/BBa_K1761001/annotation2473620/range2473620/1
type: Range
persistent identity: range2473620
display id: range2473620
version: 1
direction: direction/forward
top level: BBa_K1761001
start: 514
end: 726
orientation: inline

OmpX
type: SequenceAnnotation
persistent identity: annotation2473607
display id: annotation2473607
version: 1
top level: BBa_K1761001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1761001/annotation2473607/range2473607/1

https://synbiohub.org/public/igem/BBa_K1761001/annotation2473607/range2473607/1
type: Range
persistent identity: range2473607
display id: range2473607
version: 1
direction: direction/forward
top level: BBa_K1761001
start: 1
end: 513
orientation: inline

mNeonGreen
type: SequenceAnnotation
persistent identity: annotation2473609
display id: annotation2473609
version: 1
top level: BBa_K1761001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1761001/annotation2473609/range2473609/1

https://synbiohub.org/public/igem/BBa_K1761001/annotation2473609/range2473609/1
type: Range
persistent identity: range2473609
display id: range2473609
version: 1
direction: direction/forward
top level: BBa_K1761001
start: 727
end: 1437
orientation: inline

BBa_K777113
type: ComponentDefinition
persistent identity: BBa K777113
display id: BBa_K777113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA
creator: Team Goettingen
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: MotA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K777113_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2184144
display id: annotation2184144
version: 1
top level: BBa_K777113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184144/range2184144/1
type: Range
persistent identity: range2184144
display id: range2184144
version: 1
direction: direction/forward
top level: BBa_K777113
start: 831
end: 831
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184143
display id: annotation2184143
version: 1
top level: BBa_K777113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184143/range2184143/1
type: Range
persistent identity: range2184143
display id: range2184143
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 1
orientation: inline

MotA coding sequence
type: SequenceAnnotation
persistent identity: annotation2184142
display id: annotation2184142
version: 1
top level: BBa_K777113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1

https://synbiohub.org/public/igem/BBa_K777113/annotation2184142/range2184142/1
type: Range
persistent identity: range2184142
display id: range2184142
version: 1
direction: direction/forward
top level: BBa_K777113
start: 1
end: 831
orientation: inline

BBa_K103014
type: ComponentDefinition
persistent identity: BBa K103014
display id: BBa_K103014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA_linker_alpha_linker
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K103006
component: BBa_K103008
sequence annotation: BBa_K103006
sequence annotation: BBa_K103008
sequence: https://synbiohub.org/public/igem/BBa_K103014_sequence/1

BBa_K103006
type: Component
persistent identity: component1993510
display id: component1993510
version: 1
top level: BBa_K103014
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103008
type: Component
persistent identity: component1993514
display id: component1993514
version: 1
top level: BBa_K103014
access: public
definition: https://synbiohub.org/public/igem/BBa_K103008/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation1993510
display id: annotation1993510
version: 1
top level: BBa_K103014
location: https://synbiohub.org/public/igem/BBa_K103014/annotation1993510/range1993510/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K103014/annotation1993510/range1993510/1
type: Range
persistent identity: range1993510
display id: range1993510
version: 1
direction: direction/reverse
top level: BBa_K103014
start: 1
end: 464
orientation: inline

BBa_K103008
type: SequenceAnnotation
persistent identity: annotation1993514
display id: annotation1993514
version: 1
top level: BBa_K103014
location: https://synbiohub.org/public/igem/BBa_K103014/annotation1993514/range1993514/1
component: BBa_K103008

https://synbiohub.org/public/igem/BBa_K103014/annotation1993514/range1993514/1
type: Range
persistent identity: range1993514
display id: range1993514
version: 1
direction: direction/reverse
top level: BBa_K103014
start: 465
end: 1049
orientation: inline

BBa_K777117
type: ComponentDefinition
persistent identity: BBa K777117
display id: BBa_K777117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB
creator: Team Goettingen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGAATCAAGCGCATCC ** Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCACCTCGGTTCGGCTGATGG
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: MotB coding region
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K777117_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2184527
display id: annotation2184527
version: 1
top level: BBa_K777117
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184527/range2184527/1
type: Range
persistent identity: range2184527
display id: range2184527
version: 1
direction: direction/forward
top level: BBa_K777117
start: 927
end: 927
orientation: inline

MotB coding region
type: SequenceAnnotation
persistent identity: annotation2184525
display id: annotation2184525
version: 1
top level: BBa_K777117
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184525/range2184525/1
type: Range
persistent identity: range2184525
display id: range2184525
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 927
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184526
display id: annotation2184526
version: 1
top level: BBa_K777117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1

https://synbiohub.org/public/igem/BBa_K777117/annotation2184526/range2184526/1
type: Range
persistent identity: range2184526
display id: range2184526
version: 1
direction: direction/forward
top level: BBa_K777117
start: 1
end: 1
orientation: inline

BBa_K777115
type: ComponentDefinition
persistent identity: BBa K777115
display id: BBa_K777115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777113
sequence annotation: BBa_J23112
sequence annotation: BBa_K777113
sequence: https://synbiohub.org/public/igem/BBa_K777115_sequence/1

BBa_J23112
type: Component
persistent identity: component2189726
display id: component2189726
version: 1
top level: BBa_K777115
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_K777113
type: Component
persistent identity: component2189728
display id: component2189728
version: 1
top level: BBa_K777115
access: public
definition: BBa_K777113

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2189726
display id: annotation2189726
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777115/annotation2189726/range2189726/1
type: Range
persistent identity: range2189726
display id: range2189726
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 1
end: 35
orientation: inline

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189728
display id: annotation2189728
version: 1
top level: BBa_K777115
location: https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777115/annotation2189728/range2189728/1
type: Range
persistent identity: range2189728
display id: range2189728
version: 1
direction: direction/reverse
top level: BBa_K777115
start: 42
end: 872
orientation: inline

CP47His
type: ComponentDefinition
persistent identity: BBa K2127004
display id: BBa_K2127004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP-47-HIS TAG
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:31:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP-47-HIS TAG
mutable notes: CP-47-HIS TAG
mutable provenance: CP-47-HIS TAG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HisTag
sequence annotation: double stop codon
sequence annotation: start
sequence annotation: CP47His
sequence: https://synbiohub.org/public/igem/BBa_K2127004_sequence/1

HisTag
type: SequenceAnnotation
persistent identity: annotation2526361
display id: annotation2526361
version: 1
top level: CP47His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1
type: Range
persistent identity: range2526361
display id: range2526361
version: 1
direction: direction/forward
top level: CP47His
start: 1522
end: 1539
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2526362
display id: annotation2526362
version: 1
top level: CP47His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1
type: Range
persistent identity: range2526362
display id: range2526362
version: 1
direction: direction/forward
top level: CP47His
start: 1543
end: 1548
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2526359
display id: annotation2526359
version: 1
top level: CP47His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1
type: Range
persistent identity: range2526359
display id: range2526359
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 3
orientation: inline

CP47His
type: SequenceAnnotation
persistent identity: annotation2526360
display id: annotation2526360
version: 1
top level: CP47His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1
type: Range
persistent identity: range2526360
display id: range2526360
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 1548
orientation: inline

BBa_K633014
type: ComponentDefinition
persistent identity: BBa K633014
display id: BBa_K633014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-06-19T01:50:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator
mutable notes: asdasdadd
mutable provenance: asdasda
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13458
component: BBa_K633002
component: BBa_K633001
component: BBa_K206000
sequence annotation: BBa_I13458
sequence annotation: BBa_K633002
sequence annotation: BBa_K633001
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K633014_sequence/1

BBa_I13458
type: Component
persistent identity: component2159631
display id: component2159631
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_K633002
type: Component
persistent identity: component2159639
display id: component2159639
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K633002/1

BBa_K633001
type: Component
persistent identity: component2159642
display id: component2159642
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K633001/1

BBa_K206000
type: Component
persistent identity: component2159635
display id: component2159635
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2159631
display id: annotation2159631
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
type: Range
persistent identity: range2159631
display id: range2159631
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1
end: 1114
orientation: inline

BBa_K633002
type: SequenceAnnotation
persistent identity: annotation2159639
display id: annotation2159639
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
component: BBa_K633002

https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
type: Range
persistent identity: range2159639
display id: range2159639
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1261
end: 3173
orientation: inline

BBa_K633001
type: SequenceAnnotation
persistent identity: annotation2159642
display id: annotation2159642
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
component: BBa_K633001

https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
type: Range
persistent identity: range2159642
display id: range2159642
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 3182
end: 4057
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2159635
display id: annotation2159635
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
type: Range
persistent identity: range2159635
display id: range2159635
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1123
end: 1252
orientation: inline

BBa_K1172401
type: ComponentDefinition
persistent identity: BBa K1172401
display id: BBa_K1172401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mtrCAB - gene cluster from S. oneidensis for cytochrome expression
creator: Sebastian Grenz
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Minimal set of genes for the expression of working cytochromes. Allows the organism to transfer electrons to its outer membrane.
mutable notes: Two illegal PstI restriction sites, which had to be removed by specific primer extensions.
mutable provenance: Shewanella oneidensis MR-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site G->A
sequence annotation: start
sequence annotation: mtrB
sequence annotation: pstI site G->A
sequence annotation: mtrC
sequence annotation: mtrA
sequence: https://synbiohub.org/public/igem/BBa_K1172401_sequence/1

PstI site G->A
type: SequenceAnnotation
persistent identity: annotation2370539
display id: annotation2370539
version: 1
top level: BBa_K1172401
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370539/range2370539/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370539/range2370539/1
type: Range
persistent identity: range2370539
display id: range2370539
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1798
end: 1798
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370543
display id: annotation2370543
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370543/range2370543/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370543/range2370543/1
type: Range
persistent identity: range2370543
display id: range2370543
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2017
end: 2019
orientation: inline

mtrB
type: SequenceAnnotation
persistent identity: annotation2370542
display id: annotation2370542
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370542/range2370542/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370542/range2370542/1
type: Range
persistent identity: range2370542
display id: range2370542
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 3019
end: 5112
orientation: inline

pstI site G->A
type: SequenceAnnotation
persistent identity: annotation2370541
display id: annotation2370541
version: 1
top level: BBa_K1172401
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370541/range2370541/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370541/range2370541/1
type: Range
persistent identity: range2370541
display id: range2370541
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2083
end: 2083
orientation: inline

mtrC
type: SequenceAnnotation
persistent identity: annotation2370537
display id: annotation2370537
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370537/range2370537/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370537/range2370537/1
type: Range
persistent identity: range2370537
display id: range2370537
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1
end: 2016
orientation: inline

mtrA
type: SequenceAnnotation
persistent identity: annotation2370540
display id: annotation2370540
version: 1
top level: BBa_K1172401
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370540/range2370540/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370540/range2370540/1
type: Range
persistent identity: range2370540
display id: range2370540
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 2017
end: 3018
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370544
display id: annotation2370544
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370544/range2370544/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370544/range2370544/1
type: Range
persistent identity: range2370544
display id: range2370544
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 3019
end: 3021
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370538
display id: annotation2370538
version: 1
top level: BBa_K1172401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172401/annotation2370538/range2370538/1

https://synbiohub.org/public/igem/BBa_K1172401/annotation2370538/range2370538/1
type: Range
persistent identity: range2370538
display id: range2370538
version: 1
direction: direction/forward
top level: BBa_K1172401
start: 1
end: 6
orientation: inline

BBa_K1933102
type: ComponentDefinition
persistent identity: BBa K1933102
display id: BBa_K1933102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDcex fused to BclA with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T12:04:53Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDcex fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS:BBa_K1933004 The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321003
component: BBa_K1933000
component: BBa_K1933004
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933000
sequence annotation: BBa_K1933004
sequence: https://synbiohub.org/public/igem/BBa_K1933102_sequence/1

BBa_K1321003
type: Component
persistent identity: component2491092
display id: component2491092
version: 1
top level: BBa_K1933102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1933000
type: Component
persistent identity: component2491091
display id: component2491091
version: 1
top level: BBa_K1933102
access: public
definition: BclA-His

BBa_K1933004
type: Component
persistent identity: component2491087
display id: component2491087
version: 1
top level: BBa_K1933102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2491092
display id: annotation2491092
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491092/range2491092/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491092/range2491092/1
type: Range
persistent identity: range2491092
display id: range2491092
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 145
end: 471
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2491091
display id: annotation2491091
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
component: BBa_K1933000

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
type: Range
persistent identity: range2491091
display id: range2491091
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 61
end: 144
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2491087
display id: annotation2491087
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
type: Range
persistent identity: range2491087
display id: range2491087
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 1
end: 60
orientation: inline

BBa_K777116
type: ComponentDefinition
persistent identity: BBa K777116
display id: BBa_K777116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777113
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K777113
sequence: https://synbiohub.org/public/igem/BBa_K777116_sequence/1

BBa_K777113
type: Component
persistent identity: component2189734
display id: component2189734
version: 1
top level: BBa_K777116
access: public
definition: BBa_K777113

BBa_J23114
type: Component
persistent identity: component2189732
display id: component2189732
version: 1
top level: BBa_K777116
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2189732
display id: annotation2189732
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777116/annotation2189732/range2189732/1
type: Range
persistent identity: range2189732
display id: range2189732
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 1
end: 35
orientation: inline

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189734
display id: annotation2189734
version: 1
top level: BBa_K777116
location: https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777116/annotation2189734/range2189734/1
type: Range
persistent identity: range2189734
display id: range2189734
version: 1
direction: direction/reverse
top level: BBa_K777116
start: 42
end: 872
orientation: inline

oprF
type: ComponentDefinition
persistent identity: BBa K1172501
display id: BBa_K1172501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: By heterologous expression of the pore-forming transmembrane-protein (or porine) OprF from Pseudomona fluorescens a further optimization of the E. coli membrane will be achieved. E. coli possesses several own porines, for example OmpF and OmpC. But these naturally occurring porines are only permeable for molecules smaller than 600 Da, which decreases the range of usable mediators significantly. Opposed to that, porine OprF from P. fluorescence forms one of the biggest known pores in the outer bacterial membrane with a permeability of up to 3000 Da, thus improving the electron shuttle mediated extracellular electron transfer (ETT) and enabling the usage of alternative mediators such as riboflavin (vitamine B2).
mutable notes: Primeroverlap for Gibson-Assembly
mutable provenance: Pseudomona fluorescens genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1172501_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2370267
display id: annotation2370267
version: 1
top level: oprF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172501/annotation2370267/range2370267/1

https://synbiohub.org/public/igem/BBa_K1172501/annotation2370267/range2370267/1
type: Range
persistent identity: range2370267
display id: range2370267
version: 1
direction: direction/forward
top level: oprF
start: 1
end: 1298
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2370296
display id: annotation2370296
version: 1
top level: oprF
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172501/annotation2370296/range2370296/1

https://synbiohub.org/public/igem/BBa_K1172501/annotation2370296/range2370296/1
type: Range
persistent identity: range2370296
display id: range2370296
version: 1
direction: direction/forward
top level: oprF
start: 1296
end: 1298
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370295
display id: annotation2370295
version: 1
top level: oprF
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172501/annotation2370295/range2370295/1

https://synbiohub.org/public/igem/BBa_K1172501/annotation2370295/range2370295/1
type: Range
persistent identity: range2370295
display id: range2370295
version: 1
direction: direction/forward
top level: oprF
start: 1
end: 3
orientation: inline

BBa_K136046
type: ComponentDefinition
persistent identity: BBa K136046
display id: BBa_K136046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: envZ*
creator: alexandra bouaziz
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:03Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3215
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
mutable notes: PCR and ligation in pCR4Blunt-TOPO
mutable provenance: bacterial genome of mutated (I281S) strain for EnvZ.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: I281S T->G
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K136046_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation1994304
display id: annotation1994304
version: 1
top level: BBa_K136046
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
type: Range
persistent identity: range1994304
display id: range1994304
version: 1
direction: direction/forward
top level: BBa_K136046
start: 1
end: 3
orientation: inline

I281S T->G
type: SequenceAnnotation
persistent identity: annotation1994273
display id: annotation1994273
version: 1
top level: BBa_K136046
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
type: Range
persistent identity: range1994273
display id: range1994273
version: 1
direction: direction/forward
top level: BBa_K136046
start: 842
end: 842
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation1994303
display id: annotation1994303
version: 1
top level: BBa_K136046
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1

https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
type: Range
persistent identity: range1994303
display id: range1994303
version: 1
direction: direction/forward
top level: BBa_K136046
start: 1351
end: 1353
orientation: inline

BBa_I712024
type: ComponentDefinition
persistent identity: BBa I712024
display id: BBa_I712024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4
creator: Katja Kolar
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sequence for chemokine receptor CD4, including signal peptide.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712024_sequence/1

BBa_K1172504
type: ComponentDefinition
persistent identity: BBa K1172504
display id: BBa_K1172504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens with medium Anderson promotor and weak RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172501
component: BBa_K608007
sequence annotation: BBa_K1172501
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1172504_sequence/1

BBa_K1172501
type: Component
persistent identity: component2359421
display id: component2359421
version: 1
top level: BBa_K1172504
access: public
definition: oprF

BBa_K608007
type: Component
persistent identity: component2359420
display id: component2359420
version: 1
top level: BBa_K1172504
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1172501
type: SequenceAnnotation
persistent identity: annotation2359421
display id: annotation2359421
version: 1
top level: BBa_K1172504
location: https://synbiohub.org/public/igem/BBa_K1172504/annotation2359421/range2359421/1
component: BBa_K1172501

https://synbiohub.org/public/igem/BBa_K1172504/annotation2359421/range2359421/1
type: Range
persistent identity: range2359421
display id: range2359421
version: 1
direction: direction/reverse
top level: BBa_K1172504
start: 64
end: 1361
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2359420
display id: annotation2359420
version: 1
top level: BBa_K1172504
location: https://synbiohub.org/public/igem/BBa_K1172504/annotation2359420/range2359420/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1172504/annotation2359420/range2359420/1
type: Range
persistent identity: range2359420
display id: range2359420
version: 1
direction: direction/reverse
top level: BBa_K1172504
start: 1
end: 57
orientation: inline

BBa_K2127002
type: ComponentDefinition
persistent identity: BBa K2127002
display id: BBa_K2127002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP47 His-tagged with Lac Promoter
creator: Dawson Zeng
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:46:10Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 17480
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
mutable notes: Double Stop Codon. ORF Check
mutable provenance: NCBI Reference Sequence: NC_000911.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_R0010
component: BBa_K2127004
component: BBa_K2127007
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127004
sequence: https://synbiohub.org/public/igem/BBa_K2127002_sequence/1

BBa_R0010
type: Component
persistent identity: component2527099
display id: component2527099
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127004
type: Component
persistent identity: component2527113
display id: component2527113
version: 1
top level: BBa_K2127002
access: public
definition: CP47His

BBa_K2127007
type: Component
persistent identity: component2527114
display id: component2527114
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_B0030
type: Component
persistent identity: component2527107
display id: component2527107
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527107
display id: annotation2527107
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
type: Range
persistent identity: range2527107
display id: range2527107
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 201
end: 215
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527099
display id: annotation2527099
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
type: Range
persistent identity: range2527099
display id: range2527099
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 1
end: 200
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527114
display id: annotation2527114
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
type: Range
persistent identity: range2527114
display id: range2527114
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 1764
end: 1886
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527113
display id: annotation2527113
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
type: Range
persistent identity: range2527113
display id: range2527113
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 216
end: 1763
orientation: inline

BBa_K2127003
type: ComponentDefinition
persistent identity: BBa K2127003
display id: BBa_K2127003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbT
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T05:51:22Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 32415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: psbT
mutable notes: coding
mutable provenance: synchocystis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0032
component: BBa_K2127007
component: BBa_K2127005
component: BBa_R0010
sequence annotation: BBa_B0032
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127005
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K2127003_sequence/1

BBa_B0032
type: Component
persistent identity: component2526856
display id: component2526856
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K2127007
type: Component
persistent identity: component2526862
display id: component2526862
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_K2127005
type: Component
persistent identity: component2526861
display id: component2526861
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127005/1

BBa_R0010
type: Component
persistent identity: component2526848
display id: component2526848
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2526856
display id: annotation2526856
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526856/range2526856/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526856/range2526856/1
type: Range
persistent identity: range2526856
display id: range2526856
version: 1
direction: direction/forward
top level: BBa_K2127003
start: 201
end: 213
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2526862
display id: annotation2526862
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526862/range2526862/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526862/range2526862/1
type: Range
persistent identity: range2526862
display id: range2526862
version: 1
direction: direction/reverse
top level: BBa_K2127003
start: 433
end: 555
orientation: inline

BBa_K2127005
type: SequenceAnnotation
persistent identity: annotation2526861
display id: annotation2526861
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526861/range2526861/1
component: BBa_K2127005

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526861/range2526861/1
type: Range
persistent identity: range2526861
display id: range2526861
version: 1
direction: direction/reverse
top level: BBa_K2127003
start: 214
end: 432
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2526848
display id: annotation2526848
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526848/range2526848/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526848/range2526848/1
type: Range
persistent identity: range2526848
display id: range2526848
version: 1
direction: direction/forward
top level: BBa_K2127003
start: 1
end: 200
orientation: inline

INP-His
type: ComponentDefinition
persistent identity: BBa K1933001
display id: BBa_K1933001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INPNC fused to 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T12:46:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: true
mutable description: INPNC codes for N- and C- terminal domain of Ice-Nucleation Protein from Pseudomonas syringae, connected by a 6??His tag to be easily identified by Western blotting. [1]This is used as an anchoring motif for the cell surface display of passenger domain. INPNC does not have signal peptide for anchoring on the cell surface[2] Surface displayed passengers retain enzyme activity even when it is linked to INPNC with 6?? His tag. For more information, please visit our Wiki page.
mutable notes: N/A
mutable provenance: INPNC derived from BBa_K811005.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: 6xHis tag
sequence annotation: INPNC
sequence: https://synbiohub.org/public/igem/BBa_K1933001_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2490295
display id: annotation2490295
version: 1
top level: INP-His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1933001/annotation2490295/range2490295/1

https://synbiohub.org/public/igem/BBa_K1933001/annotation2490295/range2490295/1
type: Range
persistent identity: range2490295
display id: range2490295
version: 1
direction: direction/forward
top level: INP-His
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2514825
display id: annotation2514825
version: 1
top level: INP-His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1933001/annotation2514825/range2514825/1

https://synbiohub.org/public/igem/BBa_K1933001/annotation2514825/range2514825/1
type: Range
persistent identity: range2514825
display id: range2514825
version: 1
direction: direction/forward
top level: INP-His
start: 976
end: 978
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2490297
display id: annotation2490297
version: 1
top level: INP-His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1933001/annotation2490297/range2490297/1

https://synbiohub.org/public/igem/BBa_K1933001/annotation2490297/range2490297/1
type: Range
persistent identity: range2490297
display id: range2490297
version: 1
direction: direction/forward
top level: INP-His
start: 958
end: 975
orientation: inline

INPNC
type: SequenceAnnotation
persistent identity: annotation2490296
display id: annotation2490296
version: 1
top level: INP-His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933001/annotation2490296/range2490296/1

https://synbiohub.org/public/igem/BBa_K1933001/annotation2490296/range2490296/1
type: Range
persistent identity: range2490296
display id: range2490296
version: 1
direction: direction/forward
top level: INP-His
start: 4
end: 957
orientation: inline

BBa_K748001
type: ComponentDefinition
persistent identity: BBa K748001
display id: BBa_K748001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AgrC is a transmembrane protein, which is the sensor molecule of a typical two-component signal system in S.aureus. AgrC possesses several key amino acid motifs typical of histidine protein kinase sensor. The AgrC sensor kinase can specifically binds to corresponding AIP, which secreted only from specific S.aureus, and the composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.
mutable notes: we employed site-specific mutagenesis(AgrC-I A101T) to replace a amino acid residue in AgrC-I so that the mutated AgrC can bound to both AIP-I and AIP-IV.
mutable provenance: Staphylococcus aureus genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgrC-I A101T
sequence: https://synbiohub.org/public/igem/BBa_K748001_sequence/1

AgrC-I A101T
type: SequenceAnnotation
persistent identity: annotation2183341
display id: annotation2183341
version: 1
top level: BBa_K748001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K748001/annotation2183341/range2183341/1

https://synbiohub.org/public/igem/BBa_K748001/annotation2183341/range2183341/1
type: Range
persistent identity: range2183341
display id: range2183341
version: 1
direction: direction/forward
top level: BBa_K748001
start: 301
end: 303
orientation: inline

BBa_K808027
type: ComponentDefinition
persistent identity: BBa K808027
display id: BBa_K808027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EstA : inactive fusion protein for bacterial surface expression
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Esterase A is a inactivated membrane bound esterase from Pseudomonas putida. Its active site is mutated so it shows no more hydrolytic activity. It can be used for cell surface display like its shown in the following paper: Taek Ho Yang, Jae Gu Pan, Yeon Soo Seo, Joon Shick Rhee Use of Pseudomonas putida EstA as an Anchoring Motif for Display of a Periplasmic Enzyme on the Surface of Escherichia coli Appl Environ Microbiol. 2004 December; 70(12): 6968???6976. doi: 10.1128/AEM.70.12.6968-6976.2004
mutable notes: This certain variant of EstA should be used for cell surface expression and displaying of almost every kind of enzyme. There is no ATG at its 5' because its an C-terminal fusion protein. If you want to use you need to design it in the following way: N - Peri plasmatic signal sequence - Protein of interest - EstA - C We did not use any kind of BioBrick Assembly due to the generation of stop codons. If you would like to fuse EstA to any kind of protein, please use SOE PCR or an alternative kind of assembly strategy.
mutable provenance: Our parts derives from an established inactive EstA mutant on pEST100 vector, which was used in the following paper: Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808027_sequence/1

BBa_K1172505
type: ComponentDefinition
persistent identity: BBa K1172505
display id: BBa_K1172505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens with medium Anderson promotor and medium RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K608006
component: BBa_K1172501
sequence annotation: BBa_K608006
sequence annotation: BBa_K1172501
sequence: https://synbiohub.org/public/igem/BBa_K1172505_sequence/1

BBa_K608006
type: Component
persistent identity: component2359436
display id: component2359436
version: 1
top level: BBa_K1172505
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K1172501
type: Component
persistent identity: component2359437
display id: component2359437
version: 1
top level: BBa_K1172505
access: public
definition: oprF

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2359436
display id: annotation2359436
version: 1
top level: BBa_K1172505
location: https://synbiohub.org/public/igem/BBa_K1172505/annotation2359436/range2359436/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1172505/annotation2359436/range2359436/1
type: Range
persistent identity: range2359436
display id: range2359436
version: 1
direction: direction/reverse
top level: BBa_K1172505
start: 1
end: 56
orientation: inline

BBa_K1172501
type: SequenceAnnotation
persistent identity: annotation2359437
display id: annotation2359437
version: 1
top level: BBa_K1172505
location: https://synbiohub.org/public/igem/BBa_K1172505/annotation2359437/range2359437/1
component: BBa_K1172501

https://synbiohub.org/public/igem/BBa_K1172505/annotation2359437/range2359437/1
type: Range
persistent identity: range2359437
display id: range2359437
version: 1
direction: direction/reverse
top level: BBa_K1172505
start: 63
end: 1360
orientation: inline

BBa_K2127001
type: ComponentDefinition
persistent identity: BBa K2127001
display id: BBa_K2127001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible High Expression His-Tagged Photosystem II CP47 subunit
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:45:18Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 0
m user id: 32415
owner id: 32415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TFBS plac LacO CP47his
mutable notes: synthesized
mutable provenance: Synchocisytys
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_R0010
component: BBa_K2127007
component: BBa_K2127006
component: BBa_B0030
component: BBa_K2127004
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127004
sequence annotation: BBa_K2127006
sequence annotation: BBa_B0030
sequence annotation: BBa_K2127007
sequence: https://synbiohub.org/public/igem/BBa_K2127001_sequence/1

BBa_R0010
type: Component
persistent identity: component2527067
display id: component2527067
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127007
type: Component
persistent identity: component2527082
display id: component2527082
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_K2127006
type: Component
persistent identity: component2527066
display id: component2527066
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127006/1

BBa_B0030
type: Component
persistent identity: component2527075
display id: component2527075
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127004
type: Component
persistent identity: component2527081
display id: component2527081
version: 1
top level: BBa_K2127001
access: public
definition: CP47His

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527067
display id: annotation2527067
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
type: Range
persistent identity: range2527067
display id: range2527067
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 186
end: 385
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527081
display id: annotation2527081
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
type: Range
persistent identity: range2527081
display id: range2527081
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 401
end: 1948
orientation: inline

BBa_K2127006
type: SequenceAnnotation
persistent identity: annotation2527066
display id: annotation2527066
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
component: BBa_K2127006

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
type: Range
persistent identity: range2527066
display id: range2527066
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1
end: 185
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527075
display id: annotation2527075
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
type: Range
persistent identity: range2527075
display id: range2527075
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 386
end: 400
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527082
display id: annotation2527082
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
type: Range
persistent identity: range2527082
display id: range2527082
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1949
end: 2071
orientation: inline

BBa_K283036
type: ComponentDefinition
persistent identity: BBa K283036
display id: BBa_K283036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invasin(1-625aa)
creator: Shi Lei
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part includes a LacY coding sequence on the backbone J61002
mutable notes: BBa_J61002
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: invasin (1-625aa)
sequence: https://synbiohub.org/public/igem/BBa_K283036_sequence/1

invasin (1-625aa)
type: SequenceAnnotation
persistent identity: annotation2047678
display id: annotation2047678
version: 1
top level: BBa_K283036
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K283036/annotation2047678/range2047678/1

https://synbiohub.org/public/igem/BBa_K283036/annotation2047678/range2047678/1
type: Range
persistent identity: range2047678
display id: range2047678
version: 1
direction: direction/forward
top level: BBa_K283036
start: 1
end: 1900
orientation: inline

BBa_K1172507
type: ComponentDefinition
persistent identity: BBa K1172507
display id: BBa_K1172507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens with strong Anderson promoter and strong RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172501
component: BBa_K608002
sequence annotation: BBa_K1172501
sequence annotation: BBa_K608002
sequence: https://synbiohub.org/public/igem/BBa_K1172507_sequence/1

BBa_K1172501
type: Component
persistent identity: component2361221
display id: component2361221
version: 1
top level: BBa_K1172507
access: public
definition: oprF

BBa_K608002
type: Component
persistent identity: component2361220
display id: component2361220
version: 1
top level: BBa_K1172507
access: public
definition: https://synbiohub.org/public/igem/BBa_K608002/1

BBa_K1172501
type: SequenceAnnotation
persistent identity: annotation2361221
display id: annotation2361221
version: 1
top level: BBa_K1172507
location: https://synbiohub.org/public/igem/BBa_K1172507/annotation2361221/range2361221/1
component: BBa_K1172501

https://synbiohub.org/public/igem/BBa_K1172507/annotation2361221/range2361221/1
type: Range
persistent identity: range2361221
display id: range2361221
version: 1
direction: direction/reverse
top level: BBa_K1172507
start: 62
end: 1359
orientation: inline

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2361220
display id: annotation2361220
version: 1
top level: BBa_K1172507
location: https://synbiohub.org/public/igem/BBa_K1172507/annotation2361220/range2361220/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1172507/annotation2361220/range2361220/1
type: Range
persistent identity: range2361220
display id: range2361220
version: 1
direction: direction/reverse
top level: BBa_K1172507
start: 1
end: 55
orientation: inline

BBa_K1933103
type: ComponentDefinition
persistent identity: BBa K1933103
display id: BBa_K1933103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDclos fused to BclA with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:05:25Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDclos fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321002
component: BBa_K1933004
component: BBa_K1933000
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933000
sequence: https://synbiohub.org/public/igem/BBa_K1933103_sequence/1

BBa_K1321002
type: Component
persistent identity: component2490481
display id: component2490481
version: 1
top level: BBa_K1933103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1933004
type: Component
persistent identity: component2490476
display id: component2490476
version: 1
top level: BBa_K1933103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933000
type: Component
persistent identity: component2490480
display id: component2490480
version: 1
top level: BBa_K1933103
access: public
definition: BclA-His

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2490481
display id: annotation2490481
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490481/range2490481/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490481/range2490481/1
type: Range
persistent identity: range2490481
display id: range2490481
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 145
end: 444
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2490476
display id: annotation2490476
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490476/range2490476/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490476/range2490476/1
type: Range
persistent identity: range2490476
display id: range2490476
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 1
end: 60
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2490480
display id: annotation2490480
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490480/range2490480/1
component: BBa_K1933000

https://synbiohub.org/public/igem/BBa_K1933103/annotation2490480/range2490480/1
type: Range
persistent identity: range2490480
display id: range2490480
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 61
end: 144
orientation: inline

BBa_K1172502
type: ComponentDefinition
persistent identity: BBa K1172502
display id: BBa_K1172502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens with PT7 and RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K525998
component: BBa_K1172501
sequence annotation: BBa_K1172501
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1172502_sequence/1

BBa_K525998
type: Component
persistent identity: component2359415
display id: component2359415
version: 1
top level: BBa_K1172502
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1172501
type: Component
persistent identity: component2359416
display id: component2359416
version: 1
top level: BBa_K1172502
access: public
definition: oprF

BBa_K1172501
type: SequenceAnnotation
persistent identity: annotation2359416
display id: annotation2359416
version: 1
top level: BBa_K1172502
location: https://synbiohub.org/public/igem/BBa_K1172502/annotation2359416/range2359416/1
component: BBa_K1172501

https://synbiohub.org/public/igem/BBa_K1172502/annotation2359416/range2359416/1
type: Range
persistent identity: range2359416
display id: range2359416
version: 1
direction: direction/reverse
top level: BBa_K1172502
start: 39
end: 1336
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2359415
display id: annotation2359415
version: 1
top level: BBa_K1172502
location: https://synbiohub.org/public/igem/BBa_K1172502/annotation2359415/range2359415/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1172502/annotation2359415/range2359415/1
type: Range
persistent identity: range2359415
display id: range2359415
version: 1
direction: direction/reverse
top level: BBa_K1172502
start: 1
end: 32
orientation: inline

BBa_M12067
type: ComponentDefinition
persistent identity: BBa M12067
display id: BBa_M12067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E1
creator: Team Uptake--20.020 Group Project
created: 2009-05-12T11:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _387_
m user id: 0
owner id: 4677
owning group id: 387
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: E1 is a membrane protein that comes from HCV. The receptors on the liver recognize this protein, which leads to endocytosis of the particle displaying the E1. The protein can be used for specific targeting to the liver.
mutable notes: We had to make sure the amino acid sequence produced is the same as the amino acid sequence of E1 that we found online.
mutable provenance: It comes from the HCV genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M12067_sequence/1

BBa_K777114
type: ComponentDefinition
persistent identity: BBa K777114
display id: BBa_K777114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motA under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
mutable notes: *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
mutable provenance: * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777113
sequence annotation: BBa_K777113
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777114_sequence/1

BBa_J23100
type: Component
persistent identity: component2189705
display id: component2189705
version: 1
top level: BBa_K777114
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777113
type: Component
persistent identity: component2189707
display id: component2189707
version: 1
top level: BBa_K777114
access: public
definition: BBa_K777113

BBa_K777113
type: SequenceAnnotation
persistent identity: annotation2189707
display id: annotation2189707
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
component: BBa_K777113

https://synbiohub.org/public/igem/BBa_K777114/annotation2189707/range2189707/1
type: Range
persistent identity: range2189707
display id: range2189707
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 42
end: 872
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189705
display id: annotation2189705
version: 1
top level: BBa_K777114
location: https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777114/annotation2189705/range2189705/1
type: Range
persistent identity: range2189705
display id: range2189705
version: 1
direction: direction/reverse
top level: BBa_K777114
start: 1
end: 35
orientation: inline

BBa_K777118
type: ComponentDefinition
persistent identity: BBa K777118
display id: BBa_K777118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K777117
sequence annotation: BBa_J23100
sequence annotation: BBa_K777117
sequence: https://synbiohub.org/public/igem/BBa_K777118_sequence/1

BBa_J23100
type: Component
persistent identity: component2190662
display id: component2190662
version: 1
top level: BBa_K777118
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K777117
type: Component
persistent identity: component2190664
display id: component2190664
version: 1
top level: BBa_K777118
access: public
definition: BBa_K777117

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190662
display id: annotation2190662
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777118/annotation2190662/range2190662/1
type: Range
persistent identity: range2190662
display id: range2190662
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 1
end: 35
orientation: inline

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190664
display id: annotation2190664
version: 1
top level: BBa_K777118
location: https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777118/annotation2190664/range2190664/1
type: Range
persistent identity: range2190664
display id: range2190664
version: 1
direction: direction/reverse
top level: BBa_K777118
start: 42
end: 968
orientation: inline

BBa_K1933100
type: ComponentDefinition
persistent identity: BBa K1933100
display id: BBa_K1933100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDcex fused to INPNC with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T11:35:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: true
mutable description: CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS: The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1933004
component: BBa_K1933011
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933011
sequence annotation: BBa_K1933004
sequence: https://synbiohub.org/public/igem/BBa_K1933100_sequence/1

BBa_K1933004
type: Component
persistent identity: component2516438
display id: component2516438
version: 1
top level: BBa_K1933100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933011
type: Component
persistent identity: component2516442
display id: component2516442
version: 1
top level: BBa_K1933100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933011/1

BBa_K1321003
type: Component
persistent identity: component2516443
display id: component2516443
version: 1
top level: BBa_K1933100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2516443
display id: annotation2516443
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
type: Range
persistent identity: range2516443
display id: range2516443
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 1036
end: 1362
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2516442
display id: annotation2516442
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516442/range2516442/1
component: BBa_K1933011

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type: Range
persistent identity: range2516442
display id: range2516442
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 61
end: 1035
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2516438
display id: annotation2516438
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
type: Range
persistent identity: range2516438
display id: range2516438
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 1
end: 60
orientation: inline

BBa_K1172503
type: ComponentDefinition
persistent identity: BBa K1172503
display id: BBa_K1172503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer membrane porin OprF from Pseudomonas fluorescens with Plac and RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J04500
component: BBa_K1172501
sequence annotation: BBa_J04500
sequence annotation: BBa_K1172501
sequence: https://synbiohub.org/public/igem/BBa_K1172503_sequence/1

BBa_J04500
type: Component
persistent identity: component2359430
display id: component2359430
version: 1
top level: BBa_K1172503
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1172501
type: Component
persistent identity: component2359431
display id: component2359431
version: 1
top level: BBa_K1172503
access: public
definition: oprF

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2359430
display id: annotation2359430
version: 1
top level: BBa_K1172503
location: https://synbiohub.org/public/igem/BBa_K1172503/annotation2359430/range2359430/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1172503/annotation2359430/range2359430/1
type: Range
persistent identity: range2359430
display id: range2359430
version: 1
direction: direction/reverse
top level: BBa_K1172503
start: 1
end: 220
orientation: inline

BBa_K1172501
type: SequenceAnnotation
persistent identity: annotation2359431
display id: annotation2359431
version: 1
top level: BBa_K1172503
location: https://synbiohub.org/public/igem/BBa_K1172503/annotation2359431/range2359431/1
component: BBa_K1172501

https://synbiohub.org/public/igem/BBa_K1172503/annotation2359431/range2359431/1
type: Range
persistent identity: range2359431
display id: range2359431
version: 1
direction: direction/reverse
top level: BBa_K1172503
start: 227
end: 1524
orientation: inline

BBa_K777119
type: ComponentDefinition
persistent identity: BBa K777119
display id: BBa_K777119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777117
component: BBa_J23112
sequence annotation: BBa_J23112
sequence annotation: BBa_K777117
sequence: https://synbiohub.org/public/igem/BBa_K777119_sequence/1

BBa_K777117
type: Component
persistent identity: component2190798
display id: component2190798
version: 1
top level: BBa_K777119
access: public
definition: BBa_K777117

BBa_J23112
type: Component
persistent identity: component2190796
display id: component2190796
version: 1
top level: BBa_K777119
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2190796
display id: annotation2190796
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777119/annotation2190796/range2190796/1
type: Range
persistent identity: range2190796
display id: range2190796
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 1
end: 35
orientation: inline

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190798
display id: annotation2190798
version: 1
top level: BBa_K777119
location: https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777119/annotation2190798/range2190798/1
type: Range
persistent identity: range2190798
display id: range2190798
version: 1
direction: direction/reverse
top level: BBa_K777119
start: 42
end: 968
orientation: inline

BBa_K1761002
type: ComponentDefinition
persistent identity: BBa K1761002
display id: BBa_K1761002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer Membrane Protein X (OmpX) with BsoBI-linker and NanoLuc
creator: Laura van Smeden
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T05:40:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2183_
m user id: 25572
owner id: 25572
owning group id: 9
status: status/Planning
bookmark: false
mutable description: OmpX is an outer membrane protein with the C- and N-termini in the intracellulair domain. To be able to use OmpX as a scaffold, a non-natural amino acid needs to be introduced. This can be done by implementing the amber stop codon TAG in one of the loops of OmpX via a mutation. With a specific tRNA an azide-functionalized amino acid can be built in, which can be used for the SPAAC click chemistry reaction with DBCO functionalized groups. The BsoBI linker is a 213 bp long flexible GGSGGS linker. Using the restriction enzyme BsoBI, the linker can become 45 bp shorter. NanoLuc is a small sized and bright luciferase. Under the addition of furimazine, the NanoLuc luciferase generates a bioluminescece emmission spectrum with a maximum around 460 nm.
mutable notes: The sequence is redesigned considering codon optimalization. Also restriction sites for biobricking and classical cloning are deleted. From the BsoBI linker, the coding sequecne for GGSGGS is redesigned causing that only two BsoBI restriction sites remain.
mutable provenance: OmpX is a outer membrane protein naturally occuring in wildtype E.coli. The BsoBI linker is inspired by the article "Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Liners" by Toon H. Evers et all from 29 August 2006. NanoLuc naturally occurs in the organism Oplophorus gracilirostris and was optimalized by the company Promega.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BsoBI linker
sequence annotation: NanoLuc
sequence annotation: BBa_K1761000
sequence: https://synbiohub.org/public/igem/BBa_K1761002_sequence/1

BsoBI linker
type: SequenceAnnotation
persistent identity: annotation2443223
display id: annotation2443223
version: 1
top level: BBa_K1761002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443223/range2443223/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443223/range2443223/1
type: Range
persistent identity: range2443223
display id: range2443223
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 514
end: 726
orientation: inline

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2443224
display id: annotation2443224
version: 1
top level: BBa_K1761002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443224/range2443224/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443224/range2443224/1
type: Range
persistent identity: range2443224
display id: range2443224
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 727
end: 1239
orientation: inline

BBa_K1761000
type: SequenceAnnotation
persistent identity: annotation2443222
display id: annotation2443222
version: 1
top level: BBa_K1761002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443222/range2443222/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443222/range2443222/1
type: Range
persistent identity: range2443222
display id: range2443222
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 1
end: 513
orientation: inline

BBa_K777120
type: ComponentDefinition
persistent identity: BBa K777120
display id: BBa_K777120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: motB under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
mutable notes: * ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777117
sequence annotation: BBa_J23114
sequence annotation: BBa_K777117
sequence: https://synbiohub.org/public/igem/BBa_K777120_sequence/1

BBa_J23114
type: Component
persistent identity: component2190799
display id: component2190799
version: 1
top level: BBa_K777120
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K777117
type: Component
persistent identity: component2190801
display id: component2190801
version: 1
top level: BBa_K777120
access: public
definition: BBa_K777117

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2190799
display id: annotation2190799
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777120/annotation2190799/range2190799/1
type: Range
persistent identity: range2190799
display id: range2190799
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 1
end: 35
orientation: inline

BBa_K777117
type: SequenceAnnotation
persistent identity: annotation2190801
display id: annotation2190801
version: 1
top level: BBa_K777120
location: https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
component: BBa_K777117

https://synbiohub.org/public/igem/BBa_K777120/annotation2190801/range2190801/1
type: Range
persistent identity: range2190801
display id: range2190801
version: 1
direction: direction/reverse
top level: BBa_K777120
start: 42
end: 968
orientation: inline

BBa_K1933101
type: ComponentDefinition
persistent identity: BBa K1933101
display id: BBa_K1933101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDclos fused to INPNC with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:01:45Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDclos fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDclos) fused to surface expression anchoring domain(INPNC), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS: iGEM16_Kyoto The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1933004
component: BBa_K1321002
component: BBa_K1933011
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933011
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1933101_sequence/1

BBa_K1933004
type: Component
persistent identity: component2515614
display id: component2515614
version: 1
top level: BBa_K1933101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1321002
type: Component
persistent identity: component2515619
display id: component2515619
version: 1
top level: BBa_K1933101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1933011
type: Component
persistent identity: component2515618
display id: component2515618
version: 1
top level: BBa_K1933101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933011/1

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2515614
display id: annotation2515614
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515614/range2515614/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515614/range2515614/1
type: Range
persistent identity: range2515614
display id: range2515614
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 1
end: 60
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2515618
display id: annotation2515618
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515618/range2515618/1
component: BBa_K1933011

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515618/range2515618/1
type: Range
persistent identity: range2515618
display id: range2515618
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 61
end: 1035
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2515619
display id: annotation2515619
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515619/range2515619/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1933101/annotation2515619/range2515619/1
type: Range
persistent identity: range2515619
display id: range2515619
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 1036
end: 1335
orientation: inline

BclA-His
type: ComponentDefinition
persistent identity: BBa K1933000
display id: BBa_K1933000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal domain of BclA protein fused to 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T12:19:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part codes N-terminal domain of BclA protein which expressed on the cell surface connected by a 6??His tag to be easily identified by Western blotting.[1] Surface displayed passengers retain enzyme activity even when it is linked to BclA with 6?? His tag.
mutable notes: optimized to expression in E. coli K-12 strain
mutable provenance: NCBI accession number CAD56880 NCBI accession number CAD56878 NCBI accession number CAD56869 NCBI accession number CAD56870
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: 6xHis tag
sequence annotation: BclA
sequence: https://synbiohub.org/public/igem/BBa_K1933000_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2490248
display id: annotation2490248
version: 1
top level: BclA-His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490248/range2490248/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490248/range2490248/1
type: Range
persistent identity: range2490248
display id: range2490248
version: 1
direction: direction/forward
top level: BclA-His
start: 1
end: 3
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2490250
display id: annotation2490250
version: 1
top level: BclA-His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490250/range2490250/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490250/range2490250/1
type: Range
persistent identity: range2490250
display id: range2490250
version: 1
direction: direction/forward
top level: BclA-His
start: 67
end: 84
orientation: inline

BclA
type: SequenceAnnotation
persistent identity: annotation2490249
display id: annotation2490249
version: 1
top level: BclA-His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1933000/annotation2490249/range2490249/1

https://synbiohub.org/public/igem/BBa_K1933000/annotation2490249/range2490249/1
type: Range
persistent identity: range2490249
display id: range2490249
version: 1
direction: direction/forward
top level: BclA-His
start: 4
end: 66
orientation: inline

BBa_K227006
type: ComponentDefinition
persistent identity: BBa K227006
display id: BBa_K227006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: puc BA coding region of R. sphaeroides
creator: WashU iGEM 2009
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _326_
m user id: 0
owner id: 4645
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: puc BA is the complete LHII transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. Data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.
mutable notes: puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.
mutable provenance: PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: pucB
sequence annotation: pucA
sequence: https://synbiohub.org/public/igem/BBa_K227006_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2057320
display id: annotation2057320
version: 1
top level: BBa_K227006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057320/range2057320/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057320/range2057320/1
type: Range
persistent identity: range2057320
display id: range2057320
version: 1
direction: direction/forward
top level: BBa_K227006
start: 159
end: 163
orientation: inline

pucB
type: SequenceAnnotation
persistent identity: annotation2057319
display id: annotation2057319
version: 1
top level: BBa_K227006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057319/range2057319/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057319/range2057319/1
type: Range
persistent identity: range2057319
display id: range2057319
version: 1
direction: direction/forward
top level: BBa_K227006
start: 1
end: 156
orientation: inline

pucA
type: SequenceAnnotation
persistent identity: annotation2057321
display id: annotation2057321
version: 1
top level: BBa_K227006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K227006/annotation2057321/range2057321/1

https://synbiohub.org/public/igem/BBa_K227006/annotation2057321/range2057321/1
type: Range
persistent identity: range2057321
display id: range2057321
version: 1
direction: direction/forward
top level: BBa_K227006
start: 172
end: 336
orientation: inline

Switch
type: ComponentDefinition
persistent identity: BBa K1185005
display id: BBa_K1185005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli L-form Switch
creator: Vincent Leonardo
created: 2013-10-25T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is and engineered BioBrick derived from the BBa_K1185000 (L-form switch for ''Bacillus subtilis'')which can be used as a switch to enable the model Gram-negative bacteria ''Escherichia coli'' to switch between cell-walled rod form and wall-less L-form. The BioBrick contains homologous regions to the ftsI (homolog to pbpb in Bacillus subtilis)and murE genes to allow integration into the chromosome of E. coli via homologous recombination. Within the BioBrick there is a IPTG inducible promoter Pspac. This promoter is upstream of the murE coding sequence when integrated into the chromosome of E. coli, placing murE operon expression under the control of IPTG. The murE operon is responsible for the synthesis of enzymes involved in production of peptidoglycan synthesis. Disruption of this pathway can be utilised to down-regulate production of the cell wall. After integration of the BioBrick, the expression of murE operon is controlled by the presence, or absence, of IPTG(via the Pspacpromoter). When IPTG is present, murE operon genes are expressed and functional cell wall is produced. When IPTG is not present the cell wall is no longer produced and cells can transition to the cell wall-less L-form phenotype. The BioBrick also contains a cat gene, conferring Ampicilin resistance. This can be used to select for transformants in rod form. This BioBrick only functions in enabling switching between walled cells and wall-deficient L-forms in E. coli. We are giving opportunities for future iGEM team to work with cell wall-less E. coli. This BioBrick should work in a similar manner as the BBa_K1185000 (B. subtilis Switch BioBrick). The characterization of this BioBrick is something that future iGEM can do.
mutable notes: The BioBrick does not contain any illegal restriction sites. All of the sequences have been taken directly from E. coli genome so there is no need for codon optimization.
mutable provenance: All the sequences come from the Escherichia coli K12 genomic sequence. The ftsI section in the BioBrick was taken from end of the ftsI gene 1268-1767bp (500bp). Sequence acquired through EcoCyc (EG10341). The murE section was taken from the start of the murE gene 1-500bp. Sequence from EcoCyc (EG10621). The Pspac promoter were taken from the registry designed by the 2007 Cambridge iGEM team (BBa_1746666). Ampicilin resistance marker gene was taken from the expression vector pSTAR (gb|AF047654.1|AF047654).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: ampR
sequence annotation: ftsI Homologous region
sequence annotation: murE Homologous region
sequence annotation: BBa_1746665
sequence annotation: murE endogenous
sequence: https://synbiohub.org/public/igem/BBa_K1185005_sequence/1

ampR
type: SequenceAnnotation
persistent identity: annotation2370727
display id: annotation2370727
version: 1
top level: Switch
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185005/annotation2370727/range2370727/1

https://synbiohub.org/public/igem/BBa_K1185005/annotation2370727/range2370727/1
type: Range
persistent identity: range2370727
display id: range2370727
version: 1
direction: direction/reverse
top level: Switch
start: 501
end: 1818
orientation: inline

ftsI Homologous region
type: SequenceAnnotation
persistent identity: annotation2370726
display id: annotation2370726
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185005/annotation2370726/range2370726/1

https://synbiohub.org/public/igem/BBa_K1185005/annotation2370726/range2370726/1
type: Range
persistent identity: range2370726
display id: range2370726
version: 1
direction: direction/forward
top level: Switch
start: 1
end: 500
orientation: inline

murE Homologous region
type: SequenceAnnotation
persistent identity: annotation2370730
display id: annotation2370730
version: 1
top level: Switch
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1185005/annotation2370730/range2370730/1

https://synbiohub.org/public/igem/BBa_K1185005/annotation2370730/range2370730/1
type: Range
persistent identity: range2370730
display id: range2370730
version: 1
direction: direction/forward
top level: Switch
start: 1894
end: 2393
orientation: inline

BBa_1746665
type: SequenceAnnotation
persistent identity: annotation2370728
display id: annotation2370728
version: 1
top level: Switch
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185005/annotation2370728/range2370728/1

https://synbiohub.org/public/igem/BBa_K1185005/annotation2370728/range2370728/1
type: Range
persistent identity: range2370728
display id: range2370728
version: 1
direction: direction/forward
top level: Switch
start: 1819
end: 1876
orientation: inline

murE endogenous
type: SequenceAnnotation
persistent identity: annotation2370729
display id: annotation2370729
version: 1
top level: Switch
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185005/annotation2370729/range2370729/1

https://synbiohub.org/public/igem/BBa_K1185005/annotation2370729/range2370729/1
type: Range
persistent identity: range2370729
display id: range2370729
version: 1
direction: direction/forward
top level: Switch
start: 1877
end: 1893
orientation: inline

BBa_K533002
type: ComponentDefinition
persistent identity: BBa K533002
display id: BBa_K533002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-HIV protease site-SH3
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Added HIV protease site between OmpA and SH3 for easier release.
mutable notes: The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.
mutable provenance: OmpA is from BL21(DE3) E. Coli genomic DNA. SH3 is from human Grb2 protein. HIV protease site is from a former part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: SH3
sequence annotation: lacI
sequence annotation: T7 promoter
sequence annotation: OmpA
sequence annotation: BBa_I712015
sequence: https://synbiohub.org/public/igem/BBa_K533002_sequence/1

SH3
type: SequenceAnnotation
persistent identity: annotation2150425
display id: annotation2150425
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1
type: Range
persistent identity: range2150425
display id: range2150425
version: 1
direction: direction/forward
top level: BBa_K533002
start: 676
end: 857
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150423
display id: annotation2150423
version: 1
top level: BBa_K533002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1
type: Range
persistent identity: range2150423
display id: range2150423
version: 1
direction: direction/forward
top level: BBa_K533002
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2146913
display id: annotation2146913
version: 1
top level: BBa_K533002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1
type: Range
persistent identity: range2146913
display id: range2146913
version: 1
direction: direction/forward
top level: BBa_K533002
start: 1
end: 18
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150424
display id: annotation2150424
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1
type: Range
persistent identity: range2150424
display id: range2150424
version: 1
direction: direction/forward
top level: BBa_K533002
start: 87
end: 633
orientation: inline

BBa_I712015
type: SequenceAnnotation
persistent identity: annotation2146914
display id: annotation2146914
version: 1
top level: BBa_K533002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1
type: Range
persistent identity: range2146914
display id: range2146914
version: 1
direction: direction/forward
top level: BBa_K533002
start: 634
end: 675
orientation: inline

BBa_K283037
type: ComponentDefinition
persistent identity: BBa K283037
display id: BBa_K283037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter-rbs-GFP(LVA)- terminators
creator: Yu bin, Jiang QinQin
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the coding sequence for the green fluorescent protein with a lpp signal peptide and a ompA domain
mutable notes: BBa_K283037
mutable provenance: self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23100
component: BBa_B0010
component: BBa_J04031
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_J04031
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K283037_sequence/1

BBa_J23100
type: Component
persistent identity: component2057147
display id: component2057147
version: 1
top level: BBa_K283037
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0010
type: Component
persistent identity: component2057155
display id: component2057155
version: 1
top level: BBa_K283037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J04031
type: Component
persistent identity: component2057154
display id: component2057154
version: 1
top level: BBa_K283037
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: Component
persistent identity: component2057149
display id: component2057149
version: 1
top level: BBa_K283037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2057157
display id: component2057157
version: 1
top level: BBa_K283037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2057154
display id: annotation2057154
version: 1
top level: BBa_K283037
location: https://synbiohub.org/public/igem/BBa_K283037/annotation2057154/range2057154/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K283037/annotation2057154/range2057154/1
type: Range
persistent identity: range2057154
display id: range2057154
version: 1
direction: direction/reverse
top level: BBa_K283037
start: 62
end: 820
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2057147
display id: annotation2057147
version: 1
top level: BBa_K283037
location: https://synbiohub.org/public/igem/BBa_K283037/annotation2057147/range2057147/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K283037/annotation2057147/range2057147/1
type: Range
persistent identity: range2057147
display id: range2057147
version: 1
direction: direction/reverse
top level: BBa_K283037
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057149
display id: annotation2057149
version: 1
top level: BBa_K283037
location: https://synbiohub.org/public/igem/BBa_K283037/annotation2057149/range2057149/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283037/annotation2057149/range2057149/1
type: Range
persistent identity: range2057149
display id: range2057149
version: 1
direction: direction/reverse
top level: BBa_K283037
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2057157
display id: annotation2057157
version: 1
top level: BBa_K283037
location: https://synbiohub.org/public/igem/BBa_K283037/annotation2057157/range2057157/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283037/annotation2057157/range2057157/1
type: Range
persistent identity: range2057157
display id: range2057157
version: 1
direction: direction/forward
top level: BBa_K283037
start: 917
end: 957
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2057155
display id: annotation2057155
version: 1
top level: BBa_K283037
location: https://synbiohub.org/public/igem/BBa_K283037/annotation2057155/range2057155/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283037/annotation2057155/range2057155/1
type: Range
persistent identity: range2057155
display id: range2057155
version: 1
direction: direction/forward
top level: BBa_K283037
start: 829
end: 908
orientation: inline