//cds/enzyme/lysis
type: Collection
persistent identity: cds enzyme lysis
display id: cds_enzyme_lysis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1392971
member: BBa_K772101
member: BBa_K112301
member: BBa_K112304
member: BBa_K112303
member: BBa_K112015
member: BBa_K358004
member: BBa_K1631003
member: BBa_K112013
member: BBa_K112016
member: BBa_K112300
member: BBa_M1891
member: BBa_K1392972
member: BBa_K112302
member: BBa_K1223001
member: BBa_K748002
member: BBa_K1904000
member: BBa_K284022
member: bbp9
member: BBa_K112014
member: BBa_K112806
member: BBa_K112012

BBa_K112302
type: ComponentDefinition
persistent identity: BBa K112302
display id: BBa_K112302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda lysozyme >} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with start codon and no stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112302 ----------------------------------------------- al0001 Forward Biobricking of lambda lysozyme with start codon and no stop codon CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with start codon and no stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112302_sequence/1

BBa_K112016
type: ComponentDefinition
persistent identity: BBa K112016
display id: BBa_K112016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme gene ready to have an rbs attached in front, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112016 ---------------------------------------- bx17 Forward biobricking of {a~T4 Endolysin!} ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg bx16 Reverse biobricking of {a~T4 Endolysin!} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112016_sequence/1

BBa_K112014
type: ComponentDefinition
persistent identity: BBa K112014
display id: BBa_K112014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 lysozyme> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 lysozyme gene with a start codon, without a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112014 ---------------------------------------- bx13 Forward biobricking of {T4 Endolysin>} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx16 Reverse biobricking of {T4 Endolysin>} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112014_sequence/1

BBa_K1392972
type: ComponentDefinition
persistent identity: BBa K1392972
display id: BBa_K1392972
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Composite part fot TetLVA TetPromoter and T4
creator: Şeniz Y??ksel Burak Kızıl
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1769_
m user id: 0
owner id: 16902
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed. As the second part we added Terminator+Tetr Promoter+T4 Endolysin. For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part
mutable notes: L(+) - Arabinose is in sugar form and harmless The designed promoter has low background activity, so the membrane proteins will not damage the cells before induction. Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues. High expression of lysozyme only is toxic to bacteria. BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites. Constructed by DNA synthesis.
mutable provenance: References: //parts.igem Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565. Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1 http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs BiblioPlus Extension Error fetching PMID 9092630:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K808000
component: BBa_R0040
component: BBa_C0040
component: BBa_K112806
component: BBa_B1006
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_C0040
sequence annotation: BBa_K808000
sequence annotation: BBa_B1006
sequence annotation: BBa_K112806
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1392972_sequence/1

BBa_K808000
type: Component
persistent identity: component2415822
display id: component2415822
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_R0040
type: Component
persistent identity: component2415834
display id: component2415834
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0040
type: Component
persistent identity: component2415828
display id: component2415828
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K112806
type: Component
persistent identity: component2415840
display id: component2415840
version: 1
top level: BBa_K1392972
access: public
definition: BBa_K112806

BBa_B1006
type: Component
persistent identity: component2415833
display id: component2415833
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B0034
type: Component
persistent identity: component2415824
display id: component2415824
version: 1
top level: BBa_K1392972
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2415834
display id: annotation2415834
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415834/range2415834/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415834/range2415834/1
type: Range
persistent identity: range2415834
display id: range2415834
version: 1
direction: direction/forward
top level: BBa_K1392972
start: 1976
end: 2029
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2415828
display id: annotation2415828
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415828/range2415828/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415828/range2415828/1
type: Range
persistent identity: range2415828
display id: range2415828
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1236
end: 1920
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2415822
display id: annotation2415822
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415822/range2415822/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415822/range2415822/1
type: Range
persistent identity: range2415822
display id: range2415822
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1
end: 1209
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2415833
display id: annotation2415833
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415833/range2415833/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415833/range2415833/1
type: Range
persistent identity: range2415833
display id: range2415833
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1929
end: 1967
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2415840
display id: annotation2415840
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415840/range2415840/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415840/range2415840/1
type: Range
persistent identity: range2415840
display id: range2415840
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 2038
end: 2551
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2415824
display id: annotation2415824
version: 1
top level: BBa_K1392972
location: https://synbiohub.org/public/igem/BBa_K1392972/annotation2415824/range2415824/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1392972/annotation2415824/range2415824/1
type: Range
persistent identity: range2415824
display id: range2415824
version: 1
direction: direction/reverse
top level: BBa_K1392972
start: 1218
end: 1229
orientation: inline

BBa_K112304
type: ComponentDefinition
persistent identity: BBa K112304
display id: BBa_K112304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda lysozyme ready to have an rbs attached with a stop codon More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112304 ----------------------------------------------- al0005 Forward Biobricking of lambda lysozyme with spacer CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of lambda lysozyme with spacer CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112304_sequence/1

BBa_K112301
type: ComponentDefinition
persistent identity: BBa K112301
display id: BBa_K112301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lambda Lysozyme with stop codon and no start codon in BBb format
mutable provenance: PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112301 ----------------------------------------------- al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112301_sequence/1

BBa_K748002
type: ComponentDefinition
persistent identity: BBa K748002
display id: BBa_K748002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. Lysostaphin has activities of three enzymes namely, endo-β-N-acetyl glucosamidase, N-acteyl-muramyl-L-alanine amidase, glycylglycine endopeptidase. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the poly-glycine bridges which form cross links between glycopeptide chains in cell wall peptidoglycan of S.aureus cells. The wild-type lysostaphin gene encodes a preproenzyme, and the conversion of prolysostaphin to mature lysostaphin occurs extracellularly and involves the removal of the hydrophilic tandem repeat portion of the proenzyme. In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable notes: In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748002_sequence/1

BBa_K112012
type: ComponentDefinition
persistent identity: BBa K112012
display id: BBa_K112012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme gene with start and stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112012 ---------------------------------------- bx13 Forward biobricking of {T4 Endolysin!} ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg bx14 Reverse biobricking of {T4 Endolysin!} cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112012_sequence/1

BBa_K112013
type: ComponentDefinition
persistent identity: BBa K112013
display id: BBa_K112013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < T4 lysozyme! in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T4 lysozyme gene without a start codon, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112013 ---------------------------------------- bx15 Forward biobricking of {<T4 Endolysin!} ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg bx14 Reverse biobricking of {<T4 Endolysin!} cgttaGGATCCttatagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112013_sequence/1

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [T4 endolysin]
creator: Jin Huh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
mutable notes: High expression of lysozyme only is toxic to bacteria.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
direction: direction/forward
top level: BBa_K112806
start: 17
end: 511
orientation: inline

BBa_K284022
type: ComponentDefinition
persistent identity: BBa K284022
display id: BBa_K284022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T4 endolysin under the control of T7 promotor
creator: Ane Fernanda Beraldi Zeidler, Luige A. Llerena Calder??n and Marcos Henrique de Moraes
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _386_
m user id: 0
owner id: 4973
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This construction is a device composed of: BBa_I746911 - BBa_K112806 - Bba_B0014. This device contain the T4 endolysin, a lysozyme from enterobacteria phage, that degrades peptidoglycan layer, under the control of the T7 promoter.
mutable notes: For more accurate tests about the endolysin effectiveness, this part has to be under the control of an inducible promoter, like the T7 promoter.
mutable provenance: This device is a junction of previously designed parts: BBa_I746911 - Designed by Stefan Milde Group: iGEM07_Cambridge (2008-09-30) BBa_K112806 - Designed by Jin Huh Group: iGEM08_UC_Berkeley (2008-10-31) Bba_B0014 - Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I719005
component: BBa_K112806
component: BBa_B0011
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_K112806
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K284022_sequence/1

BBa_I719005
type: Component
persistent identity: component2058982
display id: component2058982
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K112806
type: Component
persistent identity: component2058986
display id: component2058986
version: 1
top level: BBa_K284022
access: public
definition: BBa_K112806

BBa_B0011
type: Component
persistent identity: component2058991
display id: component2058991
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0034
type: Component
persistent identity: component2058984
display id: component2058984
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2058987
display id: component2058987
version: 1
top level: BBa_K284022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2058986
display id: annotation2058986
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058986/range2058986/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K284022/annotation2058986/range2058986/1
type: Range
persistent identity: range2058986
display id: range2058986
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 52
end: 565
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058984
display id: annotation2058984
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058984/range2058984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K284022/annotation2058984/range2058984/1
type: Range
persistent identity: range2058984
display id: range2058984
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 32
end: 43
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058987
display id: annotation2058987
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058987/range2058987/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K284022/annotation2058987/range2058987/1
type: Range
persistent identity: range2058987
display id: range2058987
version: 1
direction: direction/forward
top level: BBa_K284022
start: 574
end: 614
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2058991
display id: annotation2058991
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058991/range2058991/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K284022/annotation2058991/range2058991/1
type: Range
persistent identity: range2058991
display id: range2058991
version: 1
direction: direction/forward
top level: BBa_K284022
start: 623
end: 668
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2058982
display id: annotation2058982
version: 1
top level: BBa_K284022
location: https://synbiohub.org/public/igem/BBa_K284022/annotation2058982/range2058982/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K284022/annotation2058982/range2058982/1
type: Range
persistent identity: range2058982
display id: range2058982
version: 1
direction: direction/reverse
top level: BBa_K284022
start: 1
end: 23
orientation: inline

BBa_K1631003
type: ComponentDefinition
persistent identity: BBa K1631003
display id: BBa_K1631003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tlanslational unit of Colicin Lysis Protein (for colicin-E3)
creator: Yuto Yamanaka
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T06:28:24Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] Colicins are a cytotoxins which are released to environment and kill other related strains.
mutable notes: none
mutable provenance: pColE3-CA38
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: Colicin Lysis Protein
sequence: https://synbiohub.org/public/igem/BBa_K1631003_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2453045
display id: annotation2453045
version: 1
top level: BBa_K1631003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1
type: Range
persistent identity: range2453045
display id: range2453045
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 1
end: 12
orientation: inline

Colicin Lysis Protein
type: SequenceAnnotation
persistent identity: annotation2453046
display id: annotation2453046
version: 1
top level: BBa_K1631003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1
type: Range
persistent identity: range2453046
display id: range2453046
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 13
end: 156
orientation: inline

BBa_K358004
type: ComponentDefinition
persistent identity: BBa K358004
display id: BBa_K358004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda lysis cassette[SRRz/Rz1]
creator: Ken Kajita
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part causes cell death. It contains holin/antiholin, endolysin and Rz/Rz1 genes.
mutable notes: We made point mutation and prepared wild type phage DNA.
mutable provenance: λ -Hind Ⅲ digest (product of TaKaRa Bio)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Rz1
sequence annotation: endolysin
sequence annotation: holin
sequence annotation: antiholin
sequence annotation: Rz
sequence annotation: wt-RBS
sequence: https://synbiohub.org/public/igem/BBa_K358004_sequence/1

Rz1
type: SequenceAnnotation
persistent identity: annotation2080451
display id: annotation2080451
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080451/range2080451/1
type: Range
persistent identity: range2080451
display id: range2080451
version: 1
direction: direction/forward
top level: BBa_K358004
start: 1029
end: 1211
orientation: inline

endolysin
type: SequenceAnnotation
persistent identity: annotation2080449
display id: annotation2080449
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080449/range2080449/1
type: Range
persistent identity: range2080449
display id: range2080449
version: 1
direction: direction/forward
top level: BBa_K358004
start: 336
end: 812
orientation: inline

holin
type: SequenceAnnotation
persistent identity: annotation2080448
display id: annotation2080448
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080448/range2080448/1
type: Range
persistent identity: range2080448
display id: range2080448
version: 1
direction: direction/forward
top level: BBa_K358004
start: 35
end: 352
orientation: inline

antiholin
type: SequenceAnnotation
persistent identity: annotation2080447
display id: annotation2080447
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080447/range2080447/1
type: Range
persistent identity: range2080447
display id: range2080447
version: 1
direction: direction/forward
top level: BBa_K358004
start: 29
end: 352
orientation: inline

Rz
type: SequenceAnnotation
persistent identity: annotation2080450
display id: annotation2080450
version: 1
top level: BBa_K358004
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080450/range2080450/1
type: Range
persistent identity: range2080450
display id: range2080450
version: 1
direction: direction/forward
top level: BBa_K358004
start: 809
end: 1270
orientation: inline

wt-RBS
type: SequenceAnnotation
persistent identity: annotation2080446
display id: annotation2080446
version: 1
top level: BBa_K358004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1

https://synbiohub.org/public/igem/BBa_K358004/annotation2080446/range2080446/1
type: Range
persistent identity: range2080446
display id: range2080446
version: 1
direction: direction/forward
top level: BBa_K358004
start: 2
end: 26
orientation: inline

BBa_K1223001
type: ComponentDefinition
persistent identity: BBa K1223001
display id: BBa_K1223001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P.A.S.E. 1 cassette
creator: Orr Schlesinger
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoter
mutable notes: /
mutable provenance: part is synthetic. holin and lysozime originates from lambda bacteriophage. the homology regions were taken from E.coli BL21 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: downstream homology region
sequence annotation: KanR CDS
sequence annotation: P.A.S.E 1 death operaton
sequence annotation: upstream homology region
sequence annotation: Lambda lyzosime
sequence annotation: Lambda holin CDS
sequence annotation: cI regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1223001_sequence/1

downstream homology region
type: SequenceAnnotation
persistent identity: annotation2336784
display id: annotation2336784
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1
type: Range
persistent identity: range2336784
display id: range2336784
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 2094
end: 2148
orientation: inline

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2336779
display id: annotation2336779
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1
type: Range
persistent identity: range2336779
display id: range2336779
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 234
end: 1049
orientation: inline

P.A.S.E 1 death operaton
type: SequenceAnnotation
persistent identity: annotation2336781
display id: annotation2336781
version: 1
top level: BBa_K1223001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1
type: Range
persistent identity: range2336781
display id: range2336781
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 2088
orientation: inline

upstream homology region
type: SequenceAnnotation
persistent identity: annotation2336778
display id: annotation2336778
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1
type: Range
persistent identity: range2336778
display id: range2336778
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 19
end: 73
orientation: inline

Lambda lyzosime
type: SequenceAnnotation
persistent identity: annotation2336782
display id: annotation2336782
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1
type: Range
persistent identity: range2336782
display id: range2336782
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1134
end: 1610
orientation: inline

Lambda holin CDS
type: SequenceAnnotation
persistent identity: annotation2336783
display id: annotation2336783
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1
type: Range
persistent identity: range2336783
display id: range2336783
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1631
end: 1948
orientation: inline

cI regulated promoter
type: SequenceAnnotation
persistent identity: annotation2336780
display id: annotation2336780
version: 1
top level: BBa_K1223001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1
type: Range
persistent identity: range2336780
display id: range2336780
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 1107
orientation: inline

BBa_M1891
type: ComponentDefinition
persistent identity: BBa M1891
display id: BBa_M1891
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein
creator: sarah guzman
created: 2012-03-27T11:00:00Z
modified: 2015-05-08T01:13:58Z
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: gfp
mutable notes: atgagtaaag gagaagaact tttcactgga gtggtcccag ttcttgttga attagatggc gatgttaatg ggcaaaaatt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaattttat ttgcactact gggaagctac ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttctcaagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatattttac aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa atggaataca actataactc acataatgta tacatcatgg gagacaaacc aaagaatggc atcaaagtta acttcaaaat tagacacaac attaaagatg gaagcgttca attagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttccaaagat cccaacgaaa agagagatca catgatcctt cttgagtttg taacagctgc taggattaca catggcatgg atgaactata caaa
mutable provenance: gfp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite

BBa_K1904000
type: ComponentDefinition
persistent identity: BBa K1904000
display id: BBa_K1904000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated endolysin specific for Acinetobacter baumannii.
creator: Roger Milton Rubio S??nchez, Enrique Chimal, Enrique Palomino, Brian Morteo
created: 2016-06-05T11:00:00Z
modified: 2016-06-06T05:49:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2370_
m user id: 25405
owner id: 25405
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This biobrick includes the coding sequence for a mutated version (PlyAB1) of lysAB2 which specifically targets Acinetobacter baumannii. This protein was mutated in 5 aminoacids to promote its specific binding to the baumannii species.
mutable notes: This sequence was optimised for its usage in RFC10 standard and in E. coli as chassis.
mutable provenance: Acinetobacter baumannii phage PhiAB2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K1904000_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2479891
display id: annotation2479891
version: 1
top level: BBa_K1904000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1904000/annotation2479891/range2479891/1

https://synbiohub.org/public/igem/BBa_K1904000/annotation2479891/range2479891/1
type: Range
persistent identity: range2479891
display id: range2479891
version: 1
direction: direction/forward
top level: BBa_K1904000
start: 1
end: 558
orientation: inline

BBa_K112300
type: ComponentDefinition
persistent identity: BBa K112300
display id: BBa_K112300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {lambda lysozyme!} in BBb format
creator: Aron Lau
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lambda lysozyme with start and stop codons in Bbb format
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112300_sequence/1

BBa_K112015
type: ComponentDefinition
persistent identity: BBa K112015
display id: BBa_K112015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: <T4 lysozyme> in BBb
creator: Bing Xia
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T4 lysozyme without start or stop codons, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112015 ---------------------------------------- bx15 Forward biobricking of {<T4 Endolysin>} ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg bx16 Reverse biobricking of {<T4 Endolysin>} cgttaGGATCCtagatttttatacgcgtcccaagtgcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112015_sequence/1

BBa_K112303
type: ComponentDefinition
persistent identity: BBa K112303
display id: BBa_K112303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< lambda lysozyme >} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lambda lysozyme with no start/stop codon in BBb format More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112303 ----------------------------------------------- al0003 Forward Biobricking of lambda lysozyme with no start/stop codon CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon CGttaGGATCCtacatcaatctctctgaccgttccgc
mutable provenance: pSB3C6-Bjh1501
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112303_sequence/1

BBa_K772101
type: ComponentDefinition
persistent identity: BBa K772101
display id: BBa_K772101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Self-Destruction Kit: Endolysin + Holin
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell. Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage. This composite part can be used to induce self-destruction with a regulatory device in the upstream region.
mutable notes: There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.
mutable provenance: Gene sequences of endolysin and holin are gathered from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K124014
component: BBa_K112806
sequence annotation: BBa_K112806
sequence annotation: BBa_K124014
sequence: https://synbiohub.org/public/igem/BBa_K772101_sequence/1

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BBa_K112806
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persistent identity: component2202511
display id: component2202511
version: 1
top level: BBa_K772101
access: public
definition: BBa_K112806

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2202511
display id: annotation2202511
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BBa_K124014
type: SequenceAnnotation
persistent identity: annotation2202512
display id: annotation2202512
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top level: BBa_K772101
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bbp9
type: ComponentDefinition
persistent identity: BBa K732003
display id: BBa_K732003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bbp9 - Bordetella phage lysin
creator: Joshua Fass
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _978_
m user id: 0
owner id: 9227
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Putative lysin from the Bordetella phage genome. Contains sequence similarity to ElmA, a protein in E. coli that breaks down capsid polysaccharides.
mutable notes: Isolated from phage genome, made BioBrick-compatible.
mutable provenance: PCR-amplification from isolated BPP phage genome. Described as a putative lysin in: M Liu, M Gingery, SR Doulatov, Y Liu, A Hodes, S Baker, P Davis, M Simmonds, C Churcher, K Mungall, MA Quail, A Preston, ET Harvill, DJ Maskell, FA Eiserling, J Parkhill, JF Miller. (2004). Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes. Journal of Bacteriology 186(5):1503.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K732003_sequence/1

BBa_K1392971
type: ComponentDefinition
persistent identity: BBa K1392971
display id: BBa_K1392971
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Holin+Terminator
creator: Şeniz Y??ksel İlkem Kumru
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1769_
m user id: 0
owner id: 16902
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part comes from a Bacteriophage Holin Gene pS105 This is a variation of the S gene of the "Cell Lysis Cassette." When it is paired with an activator, the cassette is expressed and cell lysis is induced. The S105 gene has the initial 6 base pairs at the beginning of the wild type S gene sequence removed. This causes cell lysis to be induced at a faster rate than the wild type. The added Terminator is artificial and large.
mutable notes: No design considerations during Holin Gene Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
mutable provenance: Brown iGEM Team by Ry Young at Texas A&M Modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K124014
component: BBa_B1006
sequence annotation: BBa_K124014
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1392971_sequence/1

BBa_K124014
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persistent identity: component2415812
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access: public
definition: https://synbiohub.org/public/igem/BBa_K124014/1

BBa_B1006
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persistent identity: component2415817
display id: component2415817
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definition: https://synbiohub.org/public/igem/BBa_B1006/1

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