//cds/enzyme/protease
type: Collection
persistent identity: cds enzyme protease
display id: cds_enzyme_protease
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K116601/1
member: https://synbiohub.org/public/igem/BBa_K2020000/1
member: https://synbiohub.org/public/igem/BBa_K772002/1
member: https://synbiohub.org/public/igem/BBa_I712077/1
member: https://synbiohub.org/public/igem/BBa_K2020005/1
member: https://synbiohub.org/public/igem/BBa_K1907006/1
member: https://synbiohub.org/public/igem/BBa_K2020001/1
member: https://synbiohub.org/public/igem/BBa_K772003/1
member: https://synbiohub.org/public/igem/BBa_M1436/1
member: https://synbiohub.org/public/igem/BBa_I712020/1
member: https://synbiohub.org/public/igem/BBa_K1907001/1
member: https://synbiohub.org/public/igem/BBa_K2020006/1
member: https://synbiohub.org/public/igem/BBa_M1437/1
member: https://synbiohub.org/public/igem/BBa_K2020002/1
member: https://synbiohub.org/public/igem/BBa_K533003/1
member: https://synbiohub.org/public/igem/BBa_K1378001/1
member: https://synbiohub.org/public/igem/BBa_K1319004/1
member: https://synbiohub.org/public/igem/BBa_K1680011/1
member: https://synbiohub.org/public/igem/BBa_K1907002/1
member: https://synbiohub.org/public/igem/BBa_K2020003/1
member: https://synbiohub.org/public/igem/BBa_I712078/1
member: https://synbiohub.org/public/igem/BBa_K772001/1
member: https://synbiohub.org/public/igem/BBa_K772100/1
member: https://synbiohub.org/public/igem/BBa_K2020004/1
member: https://synbiohub.org/public/igem/BBa_I712667/1
member: https://synbiohub.org/public/igem/BBa_K1639008/1

//cds/enzyme/methylation
type: Collection
persistent identity: cds enzyme methylation
display id: cds_enzyme_methylation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K108020/1
member: cheB

//cds/enzyme/rnap
type: Collection
persistent identity: cds enzyme rnap
display id: cds_enzyme_rnap
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I712022/1
member: https://synbiohub.org/public/igem/BBa_K1479015/1

//cds/enzyme/exonuclease
type: Collection
persistent identity: cds enzyme exonuclease
display id: cds_enzyme_exonuclease
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K093001/1
member: https://synbiohub.org/public/igem/BBa_K1479005/1

//cds/enzyme/phosphorylation
type: Collection
persistent identity: cds enzyme phosphorylation
display id: cds_enzyme_phosphorylation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J212099/1
member: https://synbiohub.org/public/igem/BBa_K283008/1
member: https://synbiohub.org/public/igem/BBa_K283020/1
member: https://synbiohub.org/public/igem/BBa_K094100/1
member: https://synbiohub.org/public/igem/BBa_K136046/1
member: pck
member: https://synbiohub.org/public/igem/BBa_J58105/1
member: https://synbiohub.org/public/igem/BBa_C0082/1
member: https://synbiohub.org/public/igem/BBa_K147000/1
member: https://synbiohub.org/public/igem/BBa_K819009/1
member: https://synbiohub.org/public/igem/BBa_K557000/1
member: https://synbiohub.org/public/igem/BBa_K091002/1
member: https://synbiohub.org/public/igem/BBa_K118015/1
member: https://synbiohub.org/public/igem/BBa_K629003/1
member: https://synbiohub.org/public/igem/BBa_K118016/1
member: https://synbiohub.org/public/igem/BBa_I752001/1
member: https://synbiohub.org/public/igem/BBa_K1412829/1
member: https://synbiohub.org/public/igem/BBa_K932000/1
member: https://synbiohub.org/public/igem/BBa_J58104/1

//cds/enzyme
type: Collection
persistent identity: cds enzyme
display id: cds_enzyme
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K567004
member: BBa_J52012
member: //cds/enzyme/lysis
member: BBa_K863015
member: BBa_M36705
member: CmR
member: BBa_M36013
member: BBa_K361011
member: BBa_K398027
member: BBa_K1679006
member: BBa_K216006
member: lipA
member: BBa_K777121
member: HO1
member: BBa_K1360001
member: BBa_K1554002
member: BBa_K2127001
member: HyaC -> E.
member: BBa_K552001
member: BBa_K1031623
member: BBa_K1031624
member: DGAT
member: BBa_K567021
member: BBa_M36007
member: HyaB -> E.
member: BBa_K1331001
member: BBa_K156011
member: BBa_K552002
member: BBa_K1122676
member: BBa_K1587000
member: BBa_K1660004
member: BBa_M36002
member: BBa_K357005
member: cyaA
member: BBa_K777122
member: BBa_K567005
member: BBa_K1554003
member: SAMsynth
member: BBa_J52013
member: ArfB
member: BBa_K1072001
member: BBa_K1199044
member: BBa_K748003
member: BBa_K527000
member: BBa_K863020
member: pck
member: BBa_K1072006
member: LuxI-His
member: BBa_I716254
member: PPK
member: BBa_K1037001
member: BBa_K2185007
member: BBa_K133071
member: //cds/enzyme/rnap
member: metK
member: //cds/enzyme/endonuclease
member: BBa_K1720000
member: BBa_K777123
member: BBa_M36006
member: lacZ a
member: BBa_K863001
member: BBa_K567009
member: BBa_K567011
member: alkB2
member: ColOx
member: BBa_K567006
member: BBa_K1031923
member: BBa_K103013
member: BBa_K1462050
member: BBa_K808025
member: BBa_K1462020
member: BBa_K633014

//cds/enzyme/chromatinremodeling
type: Collection
persistent identity: cds enzyme chromatinremodeling
display id: cds_enzyme_chromatinremodeling
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K106011/1
member: https://synbiohub.org/public/igem/BBa_K1479014/1
member: https://synbiohub.org/public/igem/BBa_K1926021/1
member: https://synbiohub.org/public/igem/BBa_K294000/1
member: https://synbiohub.org/public/igem/BBa_K106013/1
member: https://synbiohub.org/public/igem/BBa_K106012/1
member: https://synbiohub.org/public/igem/BBa_K1926012/1

//cds/enzyme/ligase
type: Collection
persistent identity: cds enzyme ligase
display id: cds_enzyme_ligase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K525009/1
member: https://synbiohub.org/public/igem/BBa_K525010/1
member: https://synbiohub.org/public/igem/BBa_K112112/1
member: https://synbiohub.org/public/igem/BBa_K112114/1
member: https://synbiohub.org/public/igem/BBa_K525005/1
member: https://synbiohub.org/public/igem/BBa_M1845/1
member: https://synbiohub.org/public/igem/BBa_K525730/1
member: https://synbiohub.org/public/igem/BBa_K525720/1
member: https://synbiohub.org/public/igem/BBa_K525006/1
member: https://synbiohub.org/public/igem/BBa_K525740/1
member: https://synbiohub.org/public/igem/BBa_K112113/1
member: https://synbiohub.org/public/igem/BBa_K525710/1
member: https://synbiohub.org/public/igem/BBa_K112111/1

//cds/enzyme/dnapolymerase
type: Collection
persistent identity: cds enzyme dnapolymerase
display id: cds_enzyme_dnapolymerase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1700001/1
member: https://synbiohub.org/public/igem/BBa_K113008/1

//cds/enzyme/rnapolymerase
type: Collection
persistent identity: cds enzyme rnapolymerase
display id: cds_enzyme_rnapolymerase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K145014/1
member: https://synbiohub.org/public/igem/BBa_K1479014/1
member: https://synbiohub.org/public/igem/BBa_K2084001/1
member: https://synbiohub.org/public/igem/BBa_K283047/1

//cds/enzyme/lysis
type: Collection
persistent identity: cds enzyme lysis
display id: cds_enzyme_lysis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1392971/1
member: https://synbiohub.org/public/igem/BBa_K772101/1
member: https://synbiohub.org/public/igem/BBa_K112301/1
member: https://synbiohub.org/public/igem/BBa_K112304/1
member: https://synbiohub.org/public/igem/BBa_K112303/1
member: https://synbiohub.org/public/igem/BBa_K112015/1
member: https://synbiohub.org/public/igem/BBa_K358004/1
member: https://synbiohub.org/public/igem/BBa_K1631003/1
member: https://synbiohub.org/public/igem/BBa_K112013/1
member: https://synbiohub.org/public/igem/BBa_K112016/1
member: https://synbiohub.org/public/igem/BBa_K112300/1
member: https://synbiohub.org/public/igem/BBa_M1891/1
member: https://synbiohub.org/public/igem/BBa_K1392972/1
member: https://synbiohub.org/public/igem/BBa_K112302/1
member: https://synbiohub.org/public/igem/BBa_K1223001/1
member: BBa_K748002
member: https://synbiohub.org/public/igem/BBa_K1904000/1
member: https://synbiohub.org/public/igem/BBa_K284022/1
member: https://synbiohub.org/public/igem/BBa_K732003/1
member: https://synbiohub.org/public/igem/BBa_K112014/1
member: https://synbiohub.org/public/igem/BBa_K112806/1
member: https://synbiohub.org/public/igem/BBa_K112012/1

//cds/enzyme/dnase
type: Collection
persistent identity: cds enzyme dnase
display id: cds_enzyme_dnase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I716209/1
member: BBa_K748003

//cds/enzyme/endonuclease
type: Collection
persistent identity: cds enzyme endonuclease
display id: cds_enzyme_endonuclease
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/cds_enzyme_endonuclease_restriction/1
member: https://synbiohub.org/public/igem/BBa_K1218010/1
member: https://synbiohub.org/public/igem/BBa_K093002/1
member: https://synbiohub.org/public/igem/BBa_K1906000/1

BBa_K552002
type: ComponentDefinition
persistent identity: BBa K552002
display id: BBa_K552002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtYB Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the binary enzyme CrtYB. CrtY (Lycopene Beta-cyclase) converts lycopene into beta-carotene and serves as the last step in beta-carotene biosynthesis in Saccharomyces cerevisiae. CrtB is acts further upstream of CrtY in the pathway and is called Phytoene Synthase. CrtB converts geranylgeranyl diphosphate to phytoene.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start Codon
sequence annotation: CrtYB
sequence annotation: PGK Promoter
sequence annotation: Stop Codon
sequence annotation: ADH1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K552002_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145065
display id: annotation2145065
version: 1
top level: BBa_K552002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145065/range2145065/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145065/range2145065/1
type: Range
persistent identity: range2145065
display id: range2145065
version: 1
direction: direction/forward
top level: BBa_K552002
start: 381
end: 383
orientation: inline

CrtYB
type: SequenceAnnotation
persistent identity: annotation2145066
display id: annotation2145066
version: 1
top level: BBa_K552002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145066/range2145066/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145066/range2145066/1
type: Range
persistent identity: range2145066
display id: range2145066
version: 1
direction: direction/forward
top level: BBa_K552002
start: 383
end: 2402
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145064
display id: annotation2145064
version: 1
top level: BBa_K552002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145064/range2145064/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145064/range2145064/1
type: Range
persistent identity: range2145064
display id: range2145064
version: 1
direction: direction/forward
top level: BBa_K552002
start: 1
end: 380
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145067
display id: annotation2145067
version: 1
top level: BBa_K552002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145067/range2145067/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145067/range2145067/1
type: Range
persistent identity: range2145067
display id: range2145067
version: 1
direction: direction/forward
top level: BBa_K552002
start: 2400
end: 2402
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145068
display id: annotation2145068
version: 1
top level: BBa_K552002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145068/range2145068/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145068/range2145068/1
type: Range
persistent identity: range2145068
display id: range2145068
version: 1
direction: direction/forward
top level: BBa_K552002
start: 2403
end: 2531
orientation: inline

cheB
type: ComponentDefinition
persistent identity: BBa C0024
display id: BBa_C0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheB chemotaxis coding sequence (protein glutamate methylesterase)
creator: MIT SBC 2004 (Ken)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CheB is part of the chemotaxis pathway in E. coli.
mutable notes: Checked (DE, VC, KN). Part ready for synthesis. 8/3/4
mutable provenance: NCBI - E. Coli strain K12 substrain MG1655
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: CheB
sequence: https://synbiohub.org/public/igem/BBa_C0024_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214017
display id: annotation2214017
version: 1
top level: cheB
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1

https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1
type: Range
persistent identity: range2214017
display id: range2214017
version: 1
direction: direction/forward
top level: cheB
start: 1054
end: 1078
orientation: inline

CheB
type: SequenceAnnotation
persistent identity: annotation1891614
display id: annotation1891614
version: 1
top level: cheB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1

https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1
type: Range
persistent identity: range1891614
display id: range1891614
version: 1
direction: direction/forward
top level: cheB
start: 1
end: 1053
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Second of two required phycocyanobilin biosynthetic genes.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: PcyA
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
direction: direction/forward
top level: BBa_I15009
start: 751
end: 775
orientation: inline

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
direction: direction/forward
top level: BBa_I15009
start: 1
end: 750
orientation: inline

ACCsynth
type: ComponentDefinition
persistent identity: BBa K417001
display id: BBa_K417001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1-aminocyclopropane-1-carboxylate (ACC) synthase
creator: Ben Porebski, Andrew Perry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet). (S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine). This enzyme is commonly referred to as ACC synthase.
mutable notes: Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity.
mutable provenance: Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Stop
sequence annotation: ACC Synth
sequence: https://synbiohub.org/public/igem/BBa_K417001_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2090375
display id: annotation2090375
version: 1
top level: ACCsynth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1
type: Range
persistent identity: range2090375
display id: range2090375
version: 1
direction: direction/forward
top level: ACCsynth
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2090377
display id: annotation2090377
version: 1
top level: ACCsynth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1
type: Range
persistent identity: range2090377
display id: range2090377
version: 1
direction: direction/forward
top level: ACCsynth
start: 1288
end: 1290
orientation: inline

ACC Synth
type: SequenceAnnotation
persistent identity: annotation2090376
display id: annotation2090376
version: 1
top level: ACCsynth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1
type: Range
persistent identity: range2090376
display id: range2090376
version: 1
direction: direction/forward
top level: ACCsynth
start: 1
end: 1290
orientation: inline

Nuc-T7
type: ComponentDefinition
persistent identity: BBa K2144000
display id: BBa_K2144000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for Nuclease regulated by T7-promoter
creator: Oskar ??hman
created: 2016-09-21T11:00:00Z
modified: 2016-10-19T01:52:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2614_
m user id: 31341
owner id: 31517
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Usage: Nuclease is an enzyme with the capability of cleaving phosphodiester bonds between nucleotides in DNA. This enzyme can be used in applications concerning degradation of DNA. In our project the aim was to evaluate and use Nuclease degrading abilities to inhibit the biofilm formation produced by Staphyloccus Auerus. The encoding part of the BioBrick is derived from BBa_K729004, made by iGEM London 2012. The T7 promoter (BBa_k525998) has been inserted to control protein expession under IPTG induction. To regulate the protein expression under T7 promoter control, E.coli containing a T7 polymerase must be used, for instance BL21(DE3). Biology: Nuclease is an extracellular enzyme found in Staphyloccus Aureus. [3] S.Aureus encodes for two Nucleases, Nuc1 and Nuc2, [3] whereof the last-mentioned is a part of this BioBrick. Nuc2 is connected to the membrane with a N-terminal anchor. [3] There are several bacterial pathogens using Nuclease to combat the immune response in the host by for instance avoiding neutrophil extracellular traps. (NETs). [1] Furthermore, since eDNA is an important component of biofilm [2] Nuc is able to work as a regulator of biofilm formation. [2] Studies have suggested that the expression of Nuc contributes to the inability to produce biofilm [2] and during biofilm formation-conditions the expression of Nuc is repressed. [3]
mutable notes: The subparts was aligned by 3A Assembly.
mutable provenance: Nuclease from Staphylococcus Aureus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K525998
component: BBa_K729004
sequence annotation: BBa_K729004
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K2144000_sequence/1

BBa_K525998
type: Component
persistent identity: component2484439
display id: component2484439
version: 1
top level: Nuc-T7
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K729004
type: Component
persistent identity: component2484440
display id: component2484440
version: 1
top level: Nuc-T7
access: public
definition: https://synbiohub.org/public/igem/BBa_K729004/1

BBa_K729004
type: SequenceAnnotation
persistent identity: annotation2484440
display id: annotation2484440
version: 1
top level: Nuc-T7
location: https://synbiohub.org/public/igem/BBa_K2144000/annotation2484440/range2484440/1
component: BBa_K729004

https://synbiohub.org/public/igem/BBa_K2144000/annotation2484440/range2484440/1
type: Range
persistent identity: range2484440
display id: range2484440
version: 1
direction: direction/reverse
top level: Nuc-T7
start: 39
end: 599
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2484439
display id: annotation2484439
version: 1
top level: Nuc-T7
location: https://synbiohub.org/public/igem/BBa_K2144000/annotation2484439/range2484439/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K2144000/annotation2484439/range2484439/1
type: Range
persistent identity: range2484439
display id: range2484439
version: 1
direction: direction/reverse
top level: Nuc-T7
start: 1
end: 32
orientation: inline

BBa_K863011
type: ComponentDefinition
persistent identity: BBa K863011
display id: BBa_K863011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) coding sequence
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: tthl
sequence annotation: TAG
sequence: https://synbiohub.org/public/igem/BBa_K863011_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2184806
display id: annotation2184806
version: 1
top level: BBa_K863011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1
type: Range
persistent identity: range2184806
display id: range2184806
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 3
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184888
display id: annotation2184888
version: 1
top level: BBa_K863011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1
type: Range
persistent identity: range2184888
display id: range2184888
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 1389
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2184889
display id: annotation2184889
version: 1
top level: BBa_K863011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1
type: Range
persistent identity: range2184889
display id: range2184889
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1387
end: 1389
orientation: inline

BBa_K1587000
type: ComponentDefinition
persistent identity: BBa K1587000
display id: BBa_K1587000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ho1 with RBS
creator: Alexandre Le Scornet
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T08:43:46Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2004_
m user id: 24491
owner id: 24598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Strong RBS (BBa_B0030). Ho1 coding region (BBa_K566022). ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB).
mutable notes: No information
mutable provenance: Ho1 comes from cyanobacteria cyanobacterium Synechocystis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_K566022
sequence annotation: BBa_K566022
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K1587000_sequence/1

BBa_B0030
type: Component
persistent identity: component2453077
display id: component2453077
version: 1
top level: BBa_K1587000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K566022
type: Component
persistent identity: component2453080
display id: component2453080
version: 1
top level: BBa_K1587000
access: public
definition: https://synbiohub.org/public/igem/BBa_K566022/1

BBa_K566022
type: SequenceAnnotation
persistent identity: annotation2453080
display id: annotation2453080
version: 1
top level: BBa_K1587000
location: https://synbiohub.org/public/igem/BBa_K1587000/annotation2453080/range2453080/1
component: BBa_K566022

https://synbiohub.org/public/igem/BBa_K1587000/annotation2453080/range2453080/1
type: Range
persistent identity: range2453080
display id: range2453080
version: 1
direction: direction/reverse
top level: BBa_K1587000
start: 22
end: 744
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2453077
display id: annotation2453077
version: 1
top level: BBa_K1587000
location: https://synbiohub.org/public/igem/BBa_K1587000/annotation2453077/range2453077/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1587000/annotation2453077/range2453077/1
type: Range
persistent identity: range2453077
display id: range2453077
version: 1
direction: direction/forward
top level: BBa_K1587000
start: 1
end: 15
orientation: inline

BBa_K777123
type: ComponentDefinition
persistent identity: BBa K777123
display id: BBa_K777123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777121
component: BBa_J23112
sequence annotation: BBa_J23112
sequence annotation: BBa_K777121
sequence: https://synbiohub.org/public/igem/BBa_K777123_sequence/1

BBa_K777121
type: Component
persistent identity: component2192774
display id: component2192774
version: 1
top level: BBa_K777123
access: public
definition: BBa_K777121

BBa_J23112
type: Component
persistent identity: component2192772
display id: component2192772
version: 1
top level: BBa_K777123
access: public
definition: https://synbiohub.org/public/igem/BBa_J23112/1

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2192772
display id: annotation2192772
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777123/annotation2192772/range2192772/1
type: Range
persistent identity: range2192772
display id: range2192772
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2192774
display id: annotation2192774
version: 1
top level: BBa_K777123
location: https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777123/annotation2192774/range2192774/1
type: Range
persistent identity: range2192774
display id: range2192774
version: 1
direction: direction/reverse
top level: BBa_K777123
start: 42
end: 809
orientation: inline

ColOx
type: ComponentDefinition
persistent identity: BBa K1313000
display id: BBa_K1313000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cholesterol Oxidase
creator: Eddie Cano
created: 2014-10-10T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 0
owner id: 19941
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This sequence codifies an enzyme, Cholesterol Oxidase. This enzyme transforms the 3-OH group from the cholesterol into 3-keto; this reaction is not directly related with the elimination of the oxidation in the 7 position of the 7-ketocholesterol molecule. However, it is an "activation" reaction that unleashes the following reactions of the synthetic metabolic pathway, this occurs at the beginning of the degradation of sterols of many microbial pathways. The first step of this reactions is the oxidation of 3B-OH group, catalyzed by extracellular cholesterol oxidase.
mutable notes: Any design considerations.
mutable provenance: This part was synthesized, this originally belongs to Rhodococcus jostii RHA1 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Colox
sequence: https://synbiohub.org/public/igem/BBa_K1313000_sequence/1

Colox
type: SequenceAnnotation
persistent identity: annotation2416926
display id: annotation2416926
version: 1
top level: ColOx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1313000/annotation2416926/range2416926/1

https://synbiohub.org/public/igem/BBa_K1313000/annotation2416926/range2416926/1
type: Range
persistent identity: range2416926
display id: range2416926
version: 1
direction: direction/forward
top level: ColOx
start: 1
end: 1806
orientation: inline

pck
type: ComponentDefinition
persistent identity: BBa K2135000
display id: BBa_K2135000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pck
creator: Harley Greene
created: 2016-08-25T11:00:00Z
modified: 2016-10-14T11:28:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2604_
m user id: 29300
owner id: 29300
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pck codes for Phosphoenolpyruvate carboxykinase (Pck), a kinase involved in gluconeogenesis in E. coli
mutable notes: No codons were modified to make this part
mutable provenance: pck gene came from E coli strain MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pck
sequence annotation: Native RBS
sequence: https://synbiohub.org/public/igem/BBa_K2135000_sequence/1

pck
type: SequenceAnnotation
persistent identity: annotation2481661
display id: annotation2481661
version: 1
top level: pck
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2135000/annotation2481661/range2481661/1

https://synbiohub.org/public/igem/BBa_K2135000/annotation2481661/range2481661/1
type: Range
persistent identity: range2481661
display id: range2481661
version: 1
direction: direction/forward
top level: pck
start: 66
end: 1686
orientation: inline

Native RBS
type: SequenceAnnotation
persistent identity: annotation2481660
display id: annotation2481660
version: 1
top level: pck
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2135000/annotation2481660/range2481660/1

https://synbiohub.org/public/igem/BBa_K2135000/annotation2481660/range2481660/1
type: Range
persistent identity: range2481660
display id: range2481660
version: 1
direction: direction/forward
top level: pck
start: 1
end: 65
orientation: inline

BBa_K389401
type: ComponentDefinition
persistent identity: BBa K389401
display id: BBa_K389401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified I746370 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part I746370
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0034
component: BBa_B0015
component: BBa_I746360
component: BBa_K389004
component: BBa_I746350
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_I746350
sequence annotation: BBa_B0015
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_K389401_sequence/1

BBa_E1010
type: Component
persistent identity: component2273129
display id: component2273129
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2273126
display id: component2273126
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2273136
display id: component2273136
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2273140
display id: component2273140
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746360
type: Component
persistent identity: component2273138
display id: component2273138
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_K389004
type: Component
persistent identity: component2273143
display id: component2273143
version: 1
top level: BBa_K389401
access: public
definition: luc

BBa_I746350
type: Component
persistent identity: component2273124
display id: component2273124
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_I746350/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273143
display id: annotation2273143
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273143/range2273143/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389401/annotation2273143/range2273143/1
type: Range
persistent identity: range2273143
display id: range2273143
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1232
end: 2887
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273140
display id: annotation2273140
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273140/range2273140/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389401/annotation2273140/range2273140/1
type: Range
persistent identity: range2273140
display id: range2273140
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1214
end: 1225
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273129
display id: annotation2273129
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273129/range2273129/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389401/annotation2273129/range2273129/1
type: Range
persistent identity: range2273129
display id: range2273129
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 264
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273126
display id: annotation2273126
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273126/range2273126/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389401/annotation2273126/range2273126/1
type: Range
persistent identity: range2273126
display id: range2273126
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 246
end: 257
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2273124
display id: annotation2273124
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273124/range2273124/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K389401/annotation2273124/range2273124/1
type: Range
persistent identity: range2273124
display id: range2273124
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1
end: 237
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273136
display id: annotation2273136
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273136/range2273136/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389401/annotation2273136/range2273136/1
type: Range
persistent identity: range2273136
display id: range2273136
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 978
end: 1106
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273138
display id: annotation2273138
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273138/range2273138/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389401/annotation2273138/range2273138/1
type: Range
persistent identity: range2273138
display id: range2273138
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1115
end: 1205
orientation: inline

BBa_K1720001
type: ComponentDefinition
persistent identity: BBa K1720001
display id: BBa_K1720001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human guanylate cyclase1,soluble, beta 3 unit
creator: YuanbinCui
created: 2015-08-08T11:00:00Z
modified: 2015-09-18T07:25:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It is important for smooth muscle relaxation in the cardiovascular system. sGC contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs.There are several different isoforms of sGC subunit ,but the cardiovascular system abundant with alpha 3 unit and beta 3 unit.
mutable notes: We used lentiviral vector to transfected alpha 3 unit and beta 3 unit to HEK293 cells together. Since soluble guanylate cyclases (sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP(cGMP) and pyrophosphate. The level of cGMP will be up regulated.The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level.
mutable provenance: We get the informations from this link: http://www.ncbi.nlm.nih.gov/gene/2983
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1720001_sequence/1

BBa_J52013
type: ComponentDefinition
persistent identity: BBa J52013
display id: BBa_J52013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dnMyD88-linker-Rluc-linker-PEST191 is a part where dnMyD88 (very important protein in signaling cascade through TLR4 receptor) is linked to Rluc (reporter) and PEST191 (degradation tag). Both linkers are made of six aminoacids.
mutable notes: dnMyD88-linker-Rluc-linker-PEST191 was made by using PCR ligation technique. It was difficult since we had some problems with primers. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: Rluc
sequence annotation: dnMyD88
sequence annotation: PEST191
sequence annotation: dnMyD88-linker-Rluc-linker-PEST191
sequence: https://synbiohub.org/public/igem/BBa_J52013_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation1902841
display id: annotation1902841
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1

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type: Range
persistent identity: range1902841
display id: range1902841
version: 1
direction: direction/forward
top level: BBa_J52013
start: 418
end: 435
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902843
display id: annotation1902843
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1

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type: Range
persistent identity: range1902843
display id: range1902843
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1369
end: 1386
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902842
display id: annotation1902842
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1

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type: Range
persistent identity: range1902842
display id: range1902842
version: 1
direction: direction/forward
top level: BBa_J52013
start: 436
end: 1368
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902840
display id: annotation1902840
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1

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type: Range
persistent identity: range1902840
display id: range1902840
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 417
orientation: inline

PEST191
type: SequenceAnnotation
persistent identity: annotation1902844
display id: annotation1902844
version: 1
top level: BBa_J52013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1

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type: Range
persistent identity: range1902844
display id: range1902844
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1387
end: 1872
orientation: inline

dnMyD88-linker-Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902848
display id: annotation1902848
version: 1
top level: BBa_J52013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1

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type: Range
persistent identity: range1902848
display id: range1902848
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 1872
orientation: inline

luc
type: ComponentDefinition
persistent identity: BBa K389004
display id: BBa_K389004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luciferase from pGL4.10[luc2]
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A luciferase from Promega's pGL4.10luc2 vector.
mutable notes: sequence of this vector is already published by Promega
mutable provenance: pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Luciferase
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389004_sequence/1

Luciferase
type: SequenceAnnotation
persistent identity: annotation2077057
display id: annotation2077057
version: 1
top level: luc
role: SO:0000316
role: feature/protein
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type: Range
persistent identity: range2077057
display id: range2077057
version: 1
direction: direction/forward
top level: luc
start: 1
end: 1653
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078364
display id: annotation2078364
version: 1
top level: luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1

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type: Range
persistent identity: range2078364
display id: range2078364
version: 1
direction: direction/forward
top level: luc
start: 1654
end: 1656
orientation: inline

BBa_K103013
type: ComponentDefinition
persistent identity: BBa K103013
display id: BBa_K103013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: linker fused to omega fragment of TEM beta-lactamase
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SacI
sequence annotation: omega
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K103013_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1986318
display id: annotation1986318
version: 1
top level: BBa_K103013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103013/annotation1986318/range1986318/1

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type: Range
persistent identity: range1986318
display id: range1986318
version: 1
direction: direction/forward
top level: BBa_K103013
start: 2
end: 7
orientation: inline

omega
type: SequenceAnnotation
persistent identity: annotation1986317
display id: annotation1986317
version: 1
top level: BBa_K103013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103013/annotation1986317/range1986317/1

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type: Range
persistent identity: range1986317
display id: range1986317
version: 1
direction: direction/forward
top level: BBa_K103013
start: 60
end: 335
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1986316
display id: annotation1986316
version: 1
top level: BBa_K103013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103013/annotation1986316/range1986316/1

https://synbiohub.org/public/igem/BBa_K103013/annotation1986316/range1986316/1
type: Range
persistent identity: range1986316
display id: range1986316
version: 1
direction: direction/forward
top level: BBa_K103013
start: 1
end: 59
orientation: inline

BBa_K1072022
type: ComponentDefinition
persistent identity: BBa K1072022
display id: BBa_K1072022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+Ndh+2TM
creator: Yichong wang, Ruichen and Junjie zhang
created: 2013-09-07T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0062
component: BBa_R0063
component: BBa_B0010
component: BBa_R0062
component: BBa_B0012
component: BBa_E0040
component: BBa_C0061
component: BBa_K1072001
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_E0040
sequence annotation: BBa_K1072001
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K1072022_sequence/1

BBa_B0034
type: Component
persistent identity: component2336190
display id: component2336190
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2336193
display id: component2336193
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0063
type: Component
persistent identity: component2336184
display id: component2336184
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0010
type: Component
persistent identity: component2336197
display id: component2336197
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component2336204
display id: component2336204
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2336246
display id: component2336246
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component2336221
display id: component2336221
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0040
type: Component
persistent identity: component2336228
display id: component2336228
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0062
type: Component
persistent identity: component2336236
display id: component2336236
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component2336209
display id: component2336209
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2336226
display id: component2336226
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2336231
display id: component2336231
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component2336211
display id: component2336211
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: Component
persistent identity: component2336244
display id: component2336244
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1072001
type: Component
persistent identity: component2336243
display id: component2336243
version: 1
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access: public
definition: BBa_K1072001

BBa_B0010
type: Component
persistent identity: component2336214
display id: component2336214
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2336229
display id: component2336229
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2336199
display id: component2336199
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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display id: component2336241
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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display id: component2336216
version: 1
top level: BBa_K1072022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2336221
display id: annotation2336221
version: 1
top level: BBa_K1072022
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component: BBa_R0062

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type: Range
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display id: range2336221
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start: 1973
end: 2027
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BBa_B0010
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display id: annotation2336229
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336229/range2336229/1
component: BBa_B0010

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display id: range2336229
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BBa_R0063
type: SequenceAnnotation
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display id: annotation2336184
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component: BBa_R0063

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display id: range2336184
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BBa_B0034
type: SequenceAnnotation
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display id: annotation2336226
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component: BBa_B0034

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BBa_B0034
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display id: annotation2336209
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336209/range2336209/1
component: BBa_B0034

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display id: range2336209
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BBa_B0010
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display id: annotation2336244
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336244/range2336244/1
component: BBa_B0010

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display id: range2336244
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BBa_R0062
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display id: annotation2336236
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336236/range2336236/1
component: BBa_R0062

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type: Range
persistent identity: range2336236
display id: range2336236
version: 1
direction: direction/forward
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end: 2973
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2336199
display id: annotation2336199
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336199/range2336199/1
component: BBa_B0012

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persistent identity: range2336199
display id: range2336199
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BBa_C0061
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display id: annotation2336211
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336211/range2336211/1
component: BBa_C0061

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display id: range2336211
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BBa_B0012
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persistent identity: annotation2336231
display id: annotation2336231
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336231/range2336231/1
component: BBa_B0012

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display id: range2336231
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2336241
display id: annotation2336241
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336241/range2336241/1
component: BBa_B0034

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display id: range2336241
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direction: direction/reverse
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BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2336204
display id: annotation2336204
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336204/range2336204/1
component: BBa_R0062

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type: Range
persistent identity: range2336204
display id: range2336204
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BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2336228
display id: annotation2336228
version: 1
top level: BBa_K1072022
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component: BBa_E0040

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type: Range
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display id: range2336228
version: 1
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2336190
display id: annotation2336190
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336190/range2336190/1
component: BBa_B0034

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type: Range
persistent identity: range2336190
display id: range2336190
version: 1
direction: direction/reverse
top level: BBa_K1072022
start: 160
end: 171
orientation: inline

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2336243
display id: annotation2336243
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336243/range2336243/1
component: BBa_K1072001

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type: Range
persistent identity: range2336243
display id: range2336243
version: 1
direction: direction/reverse
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start: 3002
end: 4306
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2336193
display id: annotation2336193
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336193/range2336193/1
component: BBa_C0062

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type: Range
persistent identity: range2336193
display id: range2336193
version: 1
direction: direction/forward
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start: 178
end: 933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2336197
display id: annotation2336197
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336197/range2336197/1
component: BBa_B0010

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type: Range
persistent identity: range2336197
display id: range2336197
version: 1
direction: direction/forward
top level: BBa_K1072022
start: 967
end: 1046
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2336214
display id: annotation2336214
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336214/range2336214/1
component: BBa_B0010

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type: Range
persistent identity: range2336214
display id: range2336214
version: 1
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start: 1836
end: 1915
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2336246
display id: annotation2336246
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336246/range2336246/1
component: BBa_B0012

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type: Range
persistent identity: range2336246
display id: range2336246
version: 1
direction: direction/forward
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start: 4403
end: 4443
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2336216
display id: annotation2336216
version: 1
top level: BBa_K1072022
location: https://synbiohub.org/public/igem/BBa_K1072022/annotation2336216/range2336216/1
component: BBa_B0012

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type: Range
persistent identity: range2336216
display id: range2336216
version: 1
direction: direction/forward
top level: BBa_K1072022
start: 1924
end: 1964
orientation: inline

SAMsynth
type: ComponentDefinition
persistent identity: BBa K417000
display id: BBa_K417000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-adenosyl methionine (SAM) synthetase
creator: Ben Porebski, Andrew Perry
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the protein coding sequence of S-adenosyl methionine synthetase, which catalyses the formation of S-adenosyl methionine synthetase (AdoMet) from ATP and methionine (ATP + L-methionine + H(2)O <=> phosphate + diphosphate + S-adenosyl-L-methionine). Other names for this enzyme are AdoMet synthetase, methionine adenosyltransferase. It is the product of the metK gene in E. coli.
mutable notes: The genomic sequence corresponding to this part does not contain any illegal restriction sites with respect to RFC10, so no mutagenesis of the native sequence was required.
mutable provenance: Part was cloned from genomic DNA of Escherichia coli strain BL21(DE3) using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: SAMsynth
sequence: https://synbiohub.org/public/igem/BBa_K417000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2085975
display id: annotation2085975
version: 1
top level: SAMsynth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085975/range2085975/1

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type: Range
persistent identity: range2085975
display id: range2085975
version: 1
direction: direction/forward
top level: SAMsynth
start: 1153
end: 1153
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2085974
display id: annotation2085974
version: 1
top level: SAMsynth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085974/range2085974/1

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type: Range
persistent identity: range2085974
display id: range2085974
version: 1
direction: direction/forward
top level: SAMsynth
start: 1
end: 1
orientation: inline

SAMsynth
type: SequenceAnnotation
persistent identity: annotation2085973
display id: annotation2085973
version: 1
top level: SAMsynth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085973/range2085973/1

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type: Range
persistent identity: range2085973
display id: range2085973
version: 1
direction: direction/forward
top level: SAMsynth
start: 1
end: 1155
orientation: inline

BBa_S04524
type: ComponentDefinition
persistent identity: BBa S04524
display id: BBa_S04524
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K361000:B0014
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:14:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0014
component: BBa_K361000
sequence annotation: BBa_B0014
sequence annotation: BBa_K361000
sequence: https://synbiohub.org/public/igem/BBa_S04524_sequence/1

BBa_B0014
type: Component
persistent identity: component2095190
display id: component2095190
version: 1
top level: BBa_S04524
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K361000
type: Component
persistent identity: component2095183
display id: component2095183
version: 1
top level: BBa_S04524
access: public
definition: Rci

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2095190
display id: annotation2095190
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095190/range2095190/1
component: BBa_B0014

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type: Range
persistent identity: range2095190
display id: range2095190
version: 1
direction: direction/reverse
top level: BBa_S04524
start: 1164
end: 1258
orientation: inline

BBa_K361000
type: SequenceAnnotation
persistent identity: annotation2095183
display id: annotation2095183
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095183/range2095183/1
component: BBa_K361000

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type: Range
persistent identity: range2095183
display id: range2095183
version: 1
direction: direction/reverse
top level: BBa_S04524
start: 1
end: 1155
orientation: inline

BBa_K622006
type: ComponentDefinition
persistent identity: BBa K622006
display id: BBa_K622006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChitinaseA1
creator: Fumitaka Hashiya
created: 2011-10-01T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
group u list: _794_
m user id: 0
owner id: 9320
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes ChiA1 and its RBS. Its start codon is not AUG but GUG.
mutable notes: Start codon of this part is GUG.
mutable provenance: Genomic DNA of Streptomyces avermitilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K622006_sequence/1

BBa_K2185007
type: ComponentDefinition
persistent identity: BBa K2185007
display id: BBa_K2185007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S.ferax alpha amylase w/ cyc promoter
creator: Broad Run iGEM Team
created: 2016-09-04T11:00:00Z
modified: 2016-09-15T01:55:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2661_
m user id: 27970
owner id: 27970
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus S.ferax that codes for an alpha amylase enzyme. This composite part contains a constitutive promoter, coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. The amylase enzyme can be expressed by cloning this part into a plasmid without a promoter built in (such as the S.cerevisiae vector pRS426).
mutable notes: Codon optimized for S.cerevisiae, EcoR1 and Spe1 sites removed.
mutable provenance: The coding sequence of this part comes from the fungus S.ferax
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K392003
component: BBa_K165002
component: BBa_K792002
component: BBa_K2185006
component: BBa_I766555
sequence annotation: BBa_K392003
sequence annotation: BBa_I766555
sequence annotation: BBa_K165002
sequence annotation: BBa_K2185006
sequence annotation: BBa_K792002
sequence: https://synbiohub.org/public/igem/BBa_K2185007_sequence/1

BBa_K392003
type: Component
persistent identity: component2483536
display id: component2483536
version: 1
top level: BBa_K2185007
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K165002
type: Component
persistent identity: component2483532
display id: component2483532
version: 1
top level: BBa_K2185007
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K792002
type: Component
persistent identity: component2483534
display id: component2483534
version: 1
top level: BBa_K2185007
access: public
definition: https://synbiohub.org/public/igem/BBa_K792002/1

BBa_K2185006
type: Component
persistent identity: component2483535
display id: component2483535
version: 1
top level: BBa_K2185007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2185006/1

BBa_I766555
type: Component
persistent identity: component2483531
display id: component2483531
version: 1
top level: BBa_K2185007
access: public
definition: https://synbiohub.org/public/igem/BBa_I766555/1

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2483536
display id: annotation2483536
version: 1
top level: BBa_K2185007
location: https://synbiohub.org/public/igem/BBa_K2185007/annotation2483536/range2483536/1
component: BBa_K392003

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type: Range
persistent identity: range2483536
display id: range2483536
version: 1
direction: direction/reverse
top level: BBa_K2185007
start: 1752
end: 1880
orientation: inline

BBa_I766555
type: SequenceAnnotation
persistent identity: annotation2483531
display id: annotation2483531
version: 1
top level: BBa_K2185007
location: https://synbiohub.org/public/igem/BBa_K2185007/annotation2483531/range2483531/1
component: BBa_I766555

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type: Range
persistent identity: range2483531
display id: range2483531
version: 1
direction: direction/reverse
top level: BBa_K2185007
start: 1
end: 244
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2483532
display id: annotation2483532
version: 1
top level: BBa_K2185007
location: https://synbiohub.org/public/igem/BBa_K2185007/annotation2483532/range2483532/1
component: BBa_K165002

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type: Range
persistent identity: range2483532
display id: range2483532
version: 1
direction: direction/reverse
top level: BBa_K2185007
start: 253
end: 270
orientation: inline

BBa_K2185006
type: SequenceAnnotation
persistent identity: annotation2483535
display id: annotation2483535
version: 1
top level: BBa_K2185007
location: https://synbiohub.org/public/igem/BBa_K2185007/annotation2483535/range2483535/1
component: BBa_K2185006

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type: Range
persistent identity: range2483535
display id: range2483535
version: 1
direction: direction/reverse
top level: BBa_K2185007
start: 339
end: 1743
orientation: inline

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2483534
display id: annotation2483534
version: 1
top level: BBa_K2185007
location: https://synbiohub.org/public/igem/BBa_K2185007/annotation2483534/range2483534/1
component: BBa_K792002

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type: Range
persistent identity: range2483534
display id: range2483534
version: 1
direction: direction/reverse
top level: BBa_K2185007
start: 279
end: 332
orientation: inline

BBa_K1031621
type: ComponentDefinition
persistent identity: BBa K1031621
display id: BBa_K1031621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23105
sequence annotation: NahF
sequence: https://synbiohub.org/public/igem/BBa_K1031621_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361696
display id: annotation2361696
version: 1
top level: BBa_K1031621
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1
type: Range
persistent identity: range2361696
display id: range2361696
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1520
end: 1648
orientation: inline

J23105
type: SequenceAnnotation
persistent identity: annotation2361694
display id: annotation2361694
version: 1
top level: BBa_K1031621
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1

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type: Range
persistent identity: range2361694
display id: range2361694
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361695
display id: annotation2361695
version: 1
top level: BBa_K1031621
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1

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type: Range
persistent identity: range2361695
display id: range2361695
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 62
end: 1513
orientation: inline

BBa_K1462030
type: ComponentDefinition
persistent identity: BBa K1462030
display id: BBa_K1462030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hbd
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462030_sequence/1

GluColi
type: ComponentDefinition
persistent identity: BBa K196014
display id: BBa_K196014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Final GluColi device.
creator: Laetitia Warny
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
mutable provenance: HfsG and HfsH sequences come from Caulobacter crescentus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K196007
component: BBa_K196005
sequence annotation: BBa_K196007
sequence annotation: BBa_K196005
sequence: https://synbiohub.org/public/igem/BBa_K196014_sequence/1

BBa_K196007
type: Component
persistent identity: component2267879
display id: component2267879
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196007/1

BBa_K196005
type: Component
persistent identity: component2267888
display id: component2267888
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196005/1

BBa_K196007
type: SequenceAnnotation
persistent identity: annotation2267879
display id: annotation2267879
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
component: BBa_K196007

https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
type: Range
persistent identity: range2267879
display id: range2267879
version: 1
direction: direction/reverse
top level: GluColi
start: 1
end: 787
orientation: inline

BBa_K196005
type: SequenceAnnotation
persistent identity: annotation2267888
display id: annotation2267888
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
component: BBa_K196005

https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
type: Range
persistent identity: range2267888
display id: range2267888
version: 1
direction: direction/reverse
top level: GluColi
start: 794
end: 2646
orientation: inline

BBa_K1199043
type: ComponentDefinition
persistent identity: BBa K1199043
display id: BBa_K1199043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DhaA31 TCP(trichlorophenol)->2,3-DCP(2,3-dichloropropano)
creator: Tian Wang
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1513_
m user id: 0
owner id: 16962
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The enzyme DhaA is of particular interest because it enables a very slow conversion of the toxic artificial compound and suspected human carcinogen 1,2,3-trichloropropane (TCP) to 2,3-dichloropropane-1-ol under laboratory conditions .And DhaA31 is a mutant of DhaA ,DhaA31 has large aromatic residues at two of the three randomized positions and at two positions modified by site-directed mutagenesis. These changes enhance activity towards TCP by decreasing the accessibility of the active site to water molecules, thus promoting the formation of an activated complex.
mutable notes: no restriction site contained
mutable provenance: DhaA is from Rhodococcus sp hydrolyzes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1199043_sequence/1

MDA
type: ComponentDefinition
persistent identity: BBa M36761
display id: BBa_M36761
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Melamine deaminase coding sequence
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.
mutable notes: The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Melamine Deaminase
sequence annotation: Stop (TAG)
sequence annotation: Start (ATG)
sequence: https://synbiohub.org/public/igem/BBa_M36761_sequence/1

Melamine Deaminase
type: SequenceAnnotation
persistent identity: annotation2329380
display id: annotation2329380
version: 1
top level: MDA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1
type: Range
persistent identity: range2329380
display id: range2329380
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 1422
orientation: inline

Stop (TAG)
type: SequenceAnnotation
persistent identity: annotation2329382
display id: annotation2329382
version: 1
top level: MDA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1
type: Range
persistent identity: range2329382
display id: range2329382
version: 1
direction: direction/forward
top level: MDA
start: 1423
end: 1425
orientation: inline

Start (ATG)
type: SequenceAnnotation
persistent identity: annotation2329381
display id: annotation2329381
version: 1
top level: MDA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1
type: Range
persistent identity: range2329381
display id: range2329381
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 3
orientation: inline

BBa_K1660008
type: ComponentDefinition
persistent identity: BBa K1660008
display id: BBa_K1660008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-B0034-bace16
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T01:15:23Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a serine protease in Bacillus nematocida target essential intestinal proteins and kill nematode under the control of a constitutive promoter.
mutable notes: We add J23100, a constitutive promoter upstream the coding sequence of bace16, so that it can express Bace16.
mutable provenance: It comes from Bacillus nematocida.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop codon
sequence annotation: B0034
sequence annotation: bace16
sequence annotation: start codon
sequence annotation: J23100
sequence: https://synbiohub.org/public/igem/BBa_K1660008_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2446537
display id: annotation2446537
version: 1
top level: BBa_K1660008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660008/annotation2446537/range2446537/1

https://synbiohub.org/public/igem/BBa_K1660008/annotation2446537/range2446537/1
type: Range
persistent identity: range2446537
display id: range2446537
version: 1
direction: direction/forward
top level: BBa_K1660008
start: 1208
end: 1210
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2446534
display id: annotation2446534
version: 1
top level: BBa_K1660008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660008/annotation2446534/range2446534/1

https://synbiohub.org/public/igem/BBa_K1660008/annotation2446534/range2446534/1
type: Range
persistent identity: range2446534
display id: range2446534
version: 1
direction: direction/forward
top level: BBa_K1660008
start: 44
end: 55
orientation: inline

bace16
type: SequenceAnnotation
persistent identity: annotation2446536
display id: annotation2446536
version: 1
top level: BBa_K1660008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660008/annotation2446536/range2446536/1

https://synbiohub.org/public/igem/BBa_K1660008/annotation2446536/range2446536/1
type: Range
persistent identity: range2446536
display id: range2446536
version: 1
direction: direction/forward
top level: BBa_K1660008
start: 56
end: 1210
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2446535
display id: annotation2446535
version: 1
top level: BBa_K1660008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660008/annotation2446535/range2446535/1

https://synbiohub.org/public/igem/BBa_K1660008/annotation2446535/range2446535/1
type: Range
persistent identity: range2446535
display id: range2446535
version: 1
direction: direction/forward
top level: BBa_K1660008
start: 62
end: 64
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2446533
display id: annotation2446533
version: 1
top level: BBa_K1660008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660008/annotation2446533/range2446533/1

https://synbiohub.org/public/igem/BBa_K1660008/annotation2446533/range2446533/1
type: Range
persistent identity: range2446533
display id: range2446533
version: 1
direction: direction/forward
top level: BBa_K1660008
start: 1
end: 35
orientation: inline

gldA
type: ComponentDefinition
persistent identity: BBa K1172201
display id: BBa_K1172201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Gene gldA encodes glycerol dehydrogenase from Escherichicha coli. The overexpression of glycerol dehydrogenase in E. coli can be used as a way for endogenous mediator production. Many derivates of glycerol dehydrogenase are small, water-soluble redoxmolecules, which have mediator properties. In enzymology, a glycerol dehydrogenase (EC 1.1.1.6) is an enzyme belonging to the family of oxireductases that catalyzes the chemical reaction: Glycerol + NAD --> Glycerone + NADH + H
mutable notes: Primeroverlap for Gibson-Assembly
mutable provenance: Escherichicha coli KRX genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gldA
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1172201_sequence/1

gldA
type: SequenceAnnotation
persistent identity: annotation2332911
display id: annotation2332911
version: 1
top level: gldA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1
type: Range
persistent identity: range2332911
display id: range2332911
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 1104
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370297
display id: annotation2370297
version: 1
top level: gldA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1
type: Range
persistent identity: range2370297
display id: range2370297
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2370298
display id: annotation2370298
version: 1
top level: gldA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1
type: Range
persistent identity: range2370298
display id: range2370298
version: 1
direction: direction/forward
top level: gldA
start: 1102
end: 1104
orientation: inline

DGAT
type: ComponentDefinition
persistent identity: BBa K836002
display id: BBa_K836002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids. Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT.
mutable notes: Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.
mutable provenance: The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B1006
component: BBa_K836001
sequence annotation: BBa_B1006
sequence annotation: BBa_K836001
sequence: https://synbiohub.org/public/igem/BBa_K836002_sequence/1

BBa_B1006
type: Component
persistent identity: component2185279
display id: component2185279
version: 1
top level: DGAT
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K836001
type: Component
persistent identity: component2185269
display id: component2185269
version: 1
top level: DGAT
access: public
definition: https://synbiohub.org/public/igem/BBa_K836001/1

BBa_B1006
type: Component
persistent identity: component2185274
display id: component2185274
version: 1
top level: DGAT
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185274
display id: annotation2185274
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185274/range2185274/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836002/annotation2185274/range2185274/1
type: Range
persistent identity: range2185274
display id: range2185274
version: 1
direction: direction/reverse
top level: DGAT
start: 1381
end: 1419
orientation: inline

BBa_K836001
type: SequenceAnnotation
persistent identity: annotation2185269
display id: annotation2185269
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185269/range2185269/1
component: BBa_K836001

https://synbiohub.org/public/igem/BBa_K836002/annotation2185269/range2185269/1
type: Range
persistent identity: range2185269
display id: range2185269
version: 1
direction: direction/reverse
top level: DGAT
start: 1
end: 1380
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185279
display id: annotation2185279
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185279/range2185279/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836002/annotation2185279/range2185279/1
type: Range
persistent identity: range2185279
display id: range2185279
version: 1
direction: direction/reverse
top level: DGAT
start: 1420
end: 1458
orientation: inline

BBa_K1037002
type: ComponentDefinition
persistent identity: BBa K1037002
display id: BBa_K1037002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sbm
creator: Zeng Jianhao
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1344_
m user id: 0
owner id: 11853
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: methylmalonyl-CoA mutase;encodes methylmalonyl-CoA epimerase which catalyzes the reversible reaction of succinyl-CoA and methylmalonyl-CoA
mutable notes: base mutation
mutable provenance: E.coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Sbm coding region
sequence annotation: TAA stop coden
sequence annotation: ATG start coden
sequence: https://synbiohub.org/public/igem/BBa_K1037002_sequence/1

Sbm coding region
type: SequenceAnnotation
persistent identity: annotation2337632
display id: annotation2337632
version: 1
top level: BBa_K1037002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1037002/annotation2337632/range2337632/1

https://synbiohub.org/public/igem/BBa_K1037002/annotation2337632/range2337632/1
type: Range
persistent identity: range2337632
display id: range2337632
version: 1
direction: direction/forward
top level: BBa_K1037002
start: 1
end: 2145
orientation: inline

TAA stop coden
type: SequenceAnnotation
persistent identity: annotation2337633
display id: annotation2337633
version: 1
top level: BBa_K1037002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1037002/annotation2337633/range2337633/1

https://synbiohub.org/public/igem/BBa_K1037002/annotation2337633/range2337633/1
type: Range
persistent identity: range2337633
display id: range2337633
version: 1
direction: direction/forward
top level: BBa_K1037002
start: 2143
end: 2145
orientation: inline

ATG start coden
type: SequenceAnnotation
persistent identity: annotation2337631
display id: annotation2337631
version: 1
top level: BBa_K1037002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1037002/annotation2337631/range2337631/1

https://synbiohub.org/public/igem/BBa_K1037002/annotation2337631/range2337631/1
type: Range
persistent identity: range2337631
display id: range2337631
version: 1
direction: direction/forward
top level: BBa_K1037002
start: 1
end: 3
orientation: inline

BBa_K1199041
type: ComponentDefinition
persistent identity: BBa K1199041
display id: BBa_K1199041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LinA gamma-HCH->1,3,4,6-tetrachloro-1,4-cyclohexadiene
creator: Tian Wang
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1513_
m user id: 0
owner id: 16962
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: LinA is cofactor-independent dehalogenase from Pseudomonas paucimobilis UT26,and can metabolize gamma-HCH into 1,3,4,6-tetrachloro-1,4-cyclohexadiene to purify water .
mutable notes: No restriction sites contained in the sequence.
mutable provenance: LinA is from Pseudomonas paucimobilis UT26
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1199041_sequence/1

BBa_K1675003
type: ComponentDefinition
persistent identity: BBa K1675003
display id: BBa_K1675003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glutamate decarboxylase A （GadA）
creator: JIAQI XU
created: 2015-09-08T11:00:00Z
modified: 2015-09-15T09:54:17Z
discontinued: false
dominant: false
group u list: _2093_
m user id: 27740
owner id: 27740
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene GadA (glutamate decarboxylase A) can be cloned from Escherichia coli str. K-12 substr. GadA is a functional gene for producing alkali. It catalyzes the proton-consuming conversion of glutamate to γ-amino butyric acid (GABA) whereas it converts glutamate to GABA by removing the α-carboxylate group of glutamate.
mutable notes: Mutation of TTT to TTC Phe.(site:129bp)
mutable provenance: Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3666180&to=3667580&strand=true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GadA Coding Region
sequence annotation: ATG Start Codon
sequence annotation: TGA Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K1675003_sequence/1

GadA Coding Region
type: SequenceAnnotation
persistent identity: annotation2456979
display id: annotation2456979
version: 1
top level: BBa_K1675003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1675003/annotation2456979/range2456979/1

https://synbiohub.org/public/igem/BBa_K1675003/annotation2456979/range2456979/1
type: Range
persistent identity: range2456979
display id: range2456979
version: 1
direction: direction/forward
top level: BBa_K1675003
start: 1
end: 1401
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2456980
display id: annotation2456980
version: 1
top level: BBa_K1675003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1675003/annotation2456980/range2456980/1

https://synbiohub.org/public/igem/BBa_K1675003/annotation2456980/range2456980/1
type: Range
persistent identity: range2456980
display id: range2456980
version: 1
direction: direction/forward
top level: BBa_K1675003
start: 1
end: 3
orientation: inline

TGA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2456981
display id: annotation2456981
version: 1
top level: BBa_K1675003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1675003/annotation2456981/range2456981/1

https://synbiohub.org/public/igem/BBa_K1675003/annotation2456981/range2456981/1
type: Range
persistent identity: range2456981
display id: range2456981
version: 1
direction: direction/forward
top level: BBa_K1675003
start: 1399
end: 1401
orientation: inline

BBa_J61032
type: ComponentDefinition
persistent identity: BBa J61032
display id: BBa_J61032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alkaline phosphatase [phoA]
creator: John Anderson
created: 2006-11-10T12:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites.
mutable notes: N/A
mutable provenance: pre-pcr ca1033F/ca1032R on Citrobacter gen (1500 bp) PCR ca1032F/R on above PCR (1500 bp, XbaI/EcoRI) Sub into pSB1A2-Bca1008 (XbaI/MfeI, 2077+935, large) Product is pSB1A2-Bca1032 ---- ca1032F Forward oligo to biobrick phoA of Citrobacter GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG ca1032R Reverse oligo to biobrick phoA of Citrobacter cagtaGAATTCccagaggcggcaTTACTTCAG ca1033F Forward constant oligo to clone phoA ttatcatcgctccgcatcctg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J61032_sequence/1

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: -35
sequence annotation: C --> T
sequence annotation: LacZ protein
sequence annotation: BBa_R0040
sequence annotation: BBa_K1468004
sequence annotation: TetR 1
sequence annotation: LacZ
sequence annotation: STOP
sequence annotation: START
sequence annotation: BBa_I732005
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

BBa_K398017
type: ComponentDefinition
persistent identity: BBa K398017
display id: BBa_K398017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LadA Protein Generator
creator: Kira Schipper and Mathias Voges
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LadA, a long-chain alkane monooxygenase from Geobacillus thermodenitrificans NG80-2, facilitates the conversion of long-chain alkanes (up to at least C36) to the corresponding primary alcohols.
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J61100
component: BBa_K398000
component: BBa_J23100
sequence annotation: BBa_K398000
sequence annotation: BBa_J61100
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K398017_sequence/1

BBa_J61100
type: Component
persistent identity: component2083501
display id: component2083501
version: 1
top level: BBa_K398017
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K398000
type: Component
persistent identity: component2083502
display id: component2083502
version: 1
top level: BBa_K398017
access: public
definition: https://synbiohub.org/public/igem/BBa_K398000/1

BBa_J23100
type: Component
persistent identity: component2083500
display id: component2083500
version: 1
top level: BBa_K398017
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K398000
type: SequenceAnnotation
persistent identity: annotation2083502
display id: annotation2083502
version: 1
top level: BBa_K398017
location: https://synbiohub.org/public/igem/BBa_K398017/annotation2083502/range2083502/1
component: BBa_K398000

https://synbiohub.org/public/igem/BBa_K398017/annotation2083502/range2083502/1
type: Range
persistent identity: range2083502
display id: range2083502
version: 1
direction: direction/reverse
top level: BBa_K398017
start: 62
end: 1387
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2083501
display id: annotation2083501
version: 1
top level: BBa_K398017
location: https://synbiohub.org/public/igem/BBa_K398017/annotation2083501/range2083501/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K398017/annotation2083501/range2083501/1
type: Range
persistent identity: range2083501
display id: range2083501
version: 1
direction: direction/reverse
top level: BBa_K398017
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2083500
display id: annotation2083500
version: 1
top level: BBa_K398017
location: https://synbiohub.org/public/igem/BBa_K398017/annotation2083500/range2083500/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K398017/annotation2083500/range2083500/1
type: Range
persistent identity: range2083500
display id: range2083500
version: 1
direction: direction/reverse
top level: BBa_K398017
start: 1
end: 35
orientation: inline

BBa_K1031622
type: ComponentDefinition
persistent identity: BBa K1031622
display id: BBa_K1031622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23106
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031622_sequence/1

J23106
type: SequenceAnnotation
persistent identity: annotation2361878
display id: annotation2361878
version: 1
top level: BBa_K1031622
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1
type: Range
persistent identity: range2361878
display id: range2361878
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361879
display id: annotation2361879
version: 1
top level: BBa_K1031622
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1
type: Range
persistent identity: range2361879
display id: range2361879
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361881
display id: annotation2361881
version: 1
top level: BBa_K1031622
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1
type: Range
persistent identity: range2361881
display id: range2361881
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1519
end: 1648
orientation: inline

BBa_K398029
type: ComponentDefinition
persistent identity: BBa K398029
display id: BBa_K398029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ALDH generator (low expression)
creator: Kira Schipper
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through β-oxidation
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: XbaI site
sequence annotation: BBa_J23100
sequence annotation: BBa_K398006
sequence annotation: stop
sequence annotation: start
sequence annotation: BBa_J61117
sequence annotation: Bt-ALDH
sequence: https://synbiohub.org/public/igem/BBa_K398029_sequence/1

XbaI site
type: SequenceAnnotation
persistent identity: annotation2108877
display id: annotation2108877
version: 1
top level: BBa_K398029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108877/range2108877/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108877/range2108877/1
type: Range
persistent identity: range2108877
display id: range2108877
version: 1
direction: direction/forward
top level: BBa_K398029
start: 37
end: 42
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2108870
display id: annotation2108870
version: 1
top level: BBa_K398029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108870/range2108870/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108870/range2108870/1
type: Range
persistent identity: range2108870
display id: range2108870
version: 1
direction: direction/reverse
top level: BBa_K398029
start: 1
end: 35
orientation: inline

BBa_K398006
type: SequenceAnnotation
persistent identity: annotation2108875
display id: annotation2108875
version: 1
top level: BBa_K398029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108875/range2108875/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108875/range2108875/1
type: Range
persistent identity: range2108875
display id: range2108875
version: 1
direction: direction/reverse
top level: BBa_K398029
start: 62
end: 1555
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2108874
display id: annotation2108874
version: 1
top level: BBa_K398029
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108874/range2108874/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108874/range2108874/1
type: Range
persistent identity: range2108874
display id: range2108874
version: 1
direction: direction/forward
top level: BBa_K398029
start: 1553
end: 1555
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2108872
display id: annotation2108872
version: 1
top level: BBa_K398029
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108872/range2108872/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108872/range2108872/1
type: Range
persistent identity: range2108872
display id: range2108872
version: 1
direction: direction/forward
top level: BBa_K398029
start: 62
end: 64
orientation: inline

BBa_J61117
type: SequenceAnnotation
persistent identity: annotation2108871
display id: annotation2108871
version: 1
top level: BBa_K398029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108871/range2108871/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108871/range2108871/1
type: Range
persistent identity: range2108871
display id: range2108871
version: 1
direction: direction/reverse
top level: BBa_K398029
start: 44
end: 55
orientation: inline

Bt-ALDH
type: SequenceAnnotation
persistent identity: annotation2108873
display id: annotation2108873
version: 1
top level: BBa_K398029
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398029/annotation2108873/range2108873/1

https://synbiohub.org/public/igem/BBa_K398029/annotation2108873/range2108873/1
type: Range
persistent identity: range2108873
display id: range2108873
version: 1
direction: direction/forward
top level: BBa_K398029
start: 62
end: 1555
orientation: inline

BBa_K1172204
type: ComponentDefinition
persistent identity: BBa K1172204
display id: BBa_K1172204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli with P lacI and RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J04500
component: BBa_K1172201
sequence annotation: BBa_K1172201
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1172204_sequence/1

BBa_J04500
type: Component
persistent identity: component2332940
display id: component2332940
version: 1
top level: BBa_K1172204
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1172201
type: Component
persistent identity: component2332942
display id: component2332942
version: 1
top level: BBa_K1172204
access: public
definition: gldA

BBa_K1172201
type: SequenceAnnotation
persistent identity: annotation2332942
display id: annotation2332942
version: 1
top level: BBa_K1172204
location: https://synbiohub.org/public/igem/BBa_K1172204/annotation2332942/range2332942/1
component: BBa_K1172201

https://synbiohub.org/public/igem/BBa_K1172204/annotation2332942/range2332942/1
type: Range
persistent identity: range2332942
display id: range2332942
version: 1
direction: direction/reverse
top level: BBa_K1172204
start: 227
end: 1330
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2332940
display id: annotation2332940
version: 1
top level: BBa_K1172204
location: https://synbiohub.org/public/igem/BBa_K1172204/annotation2332940/range2332940/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1172204/annotation2332940/range2332940/1
type: Range
persistent identity: range2332940
display id: range2332940
version: 1
direction: direction/reverse
top level: BBa_K1172204
start: 1
end: 220
orientation: inline

BBa_K1072005
type: ComponentDefinition
persistent identity: BBa K1072005
display id: BBa_K1072005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7+RBS+α-ALS+T7TM
creator: Xuenian chen, Zewei yan and Yichong wang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: α-ALS gene express acetolactate synthetase that contribute to produce diacetyl. The synthetize pathway of diacetyl, in E.coli, beginning with the central pathway metabolite pyruvate as the precursor molecule, the first step in the diacetyl biosynthetic pathway involves the condensation of two molecules of pyruvate to yield one molecule of acetolactate, and is catalyzed by acetolactate synthase (ALS).The stability of acetolactate is known to be affected by the presence of oxygen, which causes its spontaneous decarboxylation to produce diacetyl.
mutable notes: -
mutable provenance: originate from Lactococcus lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: T7 Terminator
sequence annotation: alpha-ALS Coding Region
sequence annotation: T7 Promotor
sequence: https://synbiohub.org/public/igem/BBa_K1072005_sequence/1

T7 Terminator
type: SequenceAnnotation
persistent identity: annotation2352907
display id: annotation2352907
version: 1
top level: BBa_K1072005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352907/range2352907/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352907/range2352907/1
type: Range
persistent identity: range2352907
display id: range2352907
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 1791
end: 1919
orientation: inline

alpha-ALS Coding Region
type: SequenceAnnotation
persistent identity: annotation2352912
display id: annotation2352912
version: 1
top level: BBa_K1072005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352912/range2352912/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352912/range2352912/1
type: Range
persistent identity: range2352912
display id: range2352912
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 92
end: 1756
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2352906
display id: annotation2352906
version: 1
top level: BBa_K1072005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1072005/annotation2352906/range2352906/1

https://synbiohub.org/public/igem/BBa_K1072005/annotation2352906/range2352906/1
type: Range
persistent identity: range2352906
display id: range2352906
version: 1
direction: direction/forward
top level: BBa_K1072005
start: 1
end: 19
orientation: inline

lacZ a
type: ComponentDefinition
persistent identity: BBa E0033
display id: BBa_E0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
creator: Yong-Su Jin (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: to greatly reduce the number of bases, this is only a portion of the LacZ gene
mutable notes: Restriction sites (modified) 76-79 (gcc to gca) 79-81 (gct to gca) 85-87 (gaa to gag) 106-108 (tgc to tgt) 109-111(agg to aga)
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: C
sequence annotation: T7 promoter
sequence annotation: T
sequence annotation: lacZ alpha
sequence annotation: lacZ gene fragment
sequence annotation: A
sequence annotation: G
sequence annotation: T3 promoter
sequence: https://synbiohub.org/public/igem/BBa_E0033_sequence/1

C
type: SequenceAnnotation
persistent identity: annotation308383
display id: annotation308383
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
type: Range
persistent identity: range308383
display id: range308383
version: 1
direction: direction/forward
top level: lacZ a
start: 108
end: 108
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1938940
display id: annotation1938940
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
type: Range
persistent identity: range1938940
display id: range1938940
version: 1
direction: direction/reverse
top level: lacZ a
start: 173
end: 191
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308387
display id: annotation308387
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
type: Range
persistent identity: range308387
display id: range308387
version: 1
direction: direction/forward
top level: lacZ a
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308375
display id: annotation308375
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
type: Range
persistent identity: range308375
display id: range308375
version: 1
direction: direction/forward
top level: lacZ a
start: 81
end: 81
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation308320
display id: annotation308320
version: 1
top level: lacZ a
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
type: Range
persistent identity: range308320
display id: range308320
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 348
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938941
display id: annotation1938941
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
type: Range
persistent identity: range1938941
display id: range1938941
version: 1
direction: direction/forward
top level: lacZ a
start: 199
end: 348
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation308381
display id: annotation308381
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
type: Range
persistent identity: range308381
display id: range308381
version: 1
direction: direction/forward
top level: lacZ a
start: 87
end: 87
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938942
display id: annotation1938942
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
type: Range
persistent identity: range1938942
display id: range1938942
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 15
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308388
display id: annotation308388
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
type: Range
persistent identity: range308388
display id: range308388
version: 1
direction: direction/forward
top level: lacZ a
start: 111
end: 111
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1938939
display id: annotation1938939
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
type: Range
persistent identity: range1938939
display id: range1938939
version: 1
direction: direction/forward
top level: lacZ a
start: 26
end: 35
orientation: inline

BBa_K389307
type: ComponentDefinition
persistent identity: BBa K389307
display id: BBa_K389307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a medium strong constitutive promoter.
mutable notes: medium strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_J23115
component: BBa_K389004
sequence annotation: BBa_J23115
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389307_sequence/1

BBa_B0034
type: Component
persistent identity: component2085581
display id: component2085581
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23115
type: Component
persistent identity: component2085579
display id: component2085579
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_J23115/1

BBa_K389004
type: Component
persistent identity: component2085584
display id: component2085584
version: 1
top level: BBa_K389307
access: public
definition: luc

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2085579
display id: annotation2085579
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
type: Range
persistent identity: range2085579
display id: range2085579
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085584
display id: annotation2085584
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
type: Range
persistent identity: range2085584
display id: range2085584
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085581
display id: annotation2085581
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
type: Range
persistent identity: range2085581
display id: range2085581
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 44
end: 55
orientation: inline

BBa_K527000
type: ComponentDefinition
persistent identity: BBa K527000
display id: BBa_K527000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosynthesis/Oximes/CYP79A2
creator: Eva Meier Carlsen
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _693_
m user id: 0
owner id: 8631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick BBa_K527000 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
mutable notes: Mutation at a Pst1 restriction site (nucleotide 343).
mutable provenance: Institute of Plant Biology and Biotechnology Faaculty og LIFE Science Copenhagen University Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: mutation
sequence annotation: Cyp79A2
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K527000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2130033
display id: annotation2130033
version: 1
top level: BBa_K527000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K527000/annotation2130033/range2130033/1

https://synbiohub.org/public/igem/BBa_K527000/annotation2130033/range2130033/1
type: Range
persistent identity: range2130033
display id: range2130033
version: 1
direction: direction/forward
top level: BBa_K527000
start: 1
end: 3
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2130034
display id: annotation2130034
version: 1
top level: BBa_K527000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K527000/annotation2130034/range2130034/1

https://synbiohub.org/public/igem/BBa_K527000/annotation2130034/range2130034/1
type: Range
persistent identity: range2130034
display id: range2130034
version: 1
direction: direction/forward
top level: BBa_K527000
start: 342
end: 342
orientation: inline

Cyp79A2
type: SequenceAnnotation
persistent identity: annotation2130044
display id: annotation2130044
version: 1
top level: BBa_K527000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K527000/annotation2130044/range2130044/1

https://synbiohub.org/public/igem/BBa_K527000/annotation2130044/range2130044/1
type: Range
persistent identity: range2130044
display id: range2130044
version: 1
direction: direction/forward
top level: BBa_K527000
start: 1
end: 1572
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2130035
display id: annotation2130035
version: 1
top level: BBa_K527000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K527000/annotation2130035/range2130035/1

https://synbiohub.org/public/igem/BBa_K527000/annotation2130035/range2130035/1
type: Range
persistent identity: range2130035
display id: range2130035
version: 1
direction: direction/forward
top level: BBa_K527000
start: 1570
end: 1572
orientation: inline

lipA
type: ComponentDefinition
persistent identity: BBa K836003
display id: BBa_K836003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lipA from B. cepacia (codon usage optimized for E. coli)
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit). The native signal peptide was not modified.
mutable notes: The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (not in the sequence presented here).
mutable provenance: The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization for E. coli (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K836000
component: BBa_B1006
sequence annotation: BBa_B1006
sequence annotation: BBa_K836000
sequence: https://synbiohub.org/public/igem/BBa_K836003_sequence/1

BBa_K836000
type: Component
persistent identity: component2185330
display id: component2185330
version: 1
top level: lipA
access: public
definition: LipA

BBa_B1006
type: Component
persistent identity: component2185335
display id: component2185335
version: 1
top level: lipA
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185335
display id: annotation2185335
version: 1
top level: lipA
location: https://synbiohub.org/public/igem/BBa_K836003/annotation2185335/range2185335/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836003/annotation2185335/range2185335/1
type: Range
persistent identity: range2185335
display id: range2185335
version: 1
direction: direction/reverse
top level: lipA
start: 1099
end: 1137
orientation: inline

BBa_K836000
type: SequenceAnnotation
persistent identity: annotation2185330
display id: annotation2185330
version: 1
top level: lipA
location: https://synbiohub.org/public/igem/BBa_K836003/annotation2185330/range2185330/1
component: BBa_K836000

https://synbiohub.org/public/igem/BBa_K836003/annotation2185330/range2185330/1
type: Range
persistent identity: range2185330
display id: range2185330
version: 1
direction: direction/reverse
top level: lipA
start: 1
end: 1098
orientation: inline

BBa_K1360001
type: ComponentDefinition
persistent identity: BBa K1360001
display id: BBa_K1360001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alpha-L-arabinofuranosidase ArfB
creator: Liang Jiang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1736_
m user id: 0
owner id: 22520
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a gene encoding thermostable alpha-L-arabinofuranosidase from ArfB of Clostridium stercorarium DSM8532, codon optimized for E.coli
mutable notes: codon optimized for E.coli
mutable provenance: whole genome sequencing of Clostridium stercorarium DSM8532
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: ArfB
sequence: https://synbiohub.org/public/igem/BBa_K1360001_sequence/1

ArfB
type: SequenceAnnotation
persistent identity: annotation2398037
display id: annotation2398037
version: 1
top level: BBa_K1360001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1360001/annotation2398037/range2398037/1

https://synbiohub.org/public/igem/BBa_K1360001/annotation2398037/range2398037/1
type: Range
persistent identity: range2398037
display id: range2398037
version: 1
direction: direction/forward
top level: BBa_K1360001
start: 1
end: 1473
orientation: inline

BBa_K567019
type: ComponentDefinition
persistent identity: BBa K567019
display id: BBa_K567019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-6AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 6 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNAArg (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 6 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28a. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ATG
sequence annotation: PT7
sequence annotation: rbs
sequence annotation: lac operator
sequence annotation: luciferase-6AGG
sequence annotation: 6AGG
sequence: https://synbiohub.org/public/igem/BBa_K567019_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2154329
display id: annotation2154329
version: 1
top level: BBa_K567019
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154329/range2154329/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154329/range2154329/1
type: Range
persistent identity: range2154329
display id: range2154329
version: 1
direction: direction/forward
top level: BBa_K567019
start: 89
end: 91
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2154324
display id: annotation2154324
version: 1
top level: BBa_K567019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154324/range2154324/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154324/range2154324/1
type: Range
persistent identity: range2154324
display id: range2154324
version: 1
direction: direction/forward
top level: BBa_K567019
start: 2
end: 18
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154327
display id: annotation2154327
version: 1
top level: BBa_K567019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154327/range2154327/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154327/range2154327/1
type: Range
persistent identity: range2154327
display id: range2154327
version: 1
direction: direction/forward
top level: BBa_K567019
start: 75
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2154325
display id: annotation2154325
version: 1
top level: BBa_K567019
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154325/range2154325/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154325/range2154325/1
type: Range
persistent identity: range2154325
display id: range2154325
version: 1
direction: direction/forward
top level: BBa_K567019
start: 21
end: 44
orientation: inline

luciferase-6AGG
type: SequenceAnnotation
persistent identity: annotation2154337
display id: annotation2154337
version: 1
top level: BBa_K567019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154337/range2154337/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154337/range2154337/1
type: Range
persistent identity: range2154337
display id: range2154337
version: 1
direction: direction/forward
top level: BBa_K567019
start: 89
end: 1768
orientation: inline

6AGG
type: SequenceAnnotation
persistent identity: annotation2154336
display id: annotation2154336
version: 1
top level: BBa_K567019
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567019/annotation2154336/range2154336/1

https://synbiohub.org/public/igem/BBa_K567019/annotation2154336/range2154336/1
type: Range
persistent identity: range2154336
display id: range2154336
version: 1
direction: direction/forward
top level: BBa_K567019
start: 95
end: 112
orientation: inline

BBa_K567004
type: ComponentDefinition
persistent identity: BBa K567004
display id: BBa_K567004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbla-Luc-2AGG
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: β-lactamase promoter-Luciferase with two AGG-codon insertions. This biobrick is constructed by putting modified enzyme luciferase under constituitive promoter β-lactamase promoter. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg)(BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin and 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: Point mutation is used to obtain this part from wild type
mutable provenance: β-lactamase promoter is derived from pUC18 β-lactamase operon. Wild type Luciferase from BBa_I712019
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: luciferase-2AGG
sequence annotation: 2AGG
sequence annotation: ATG
sequence annotation: Pbla
sequence: https://synbiohub.org/public/igem/BBa_K567004_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2154550
display id: annotation2154550
version: 1
top level: BBa_K567004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567004/annotation2154550/range2154550/1

https://synbiohub.org/public/igem/BBa_K567004/annotation2154550/range2154550/1
type: Range
persistent identity: range2154550
display id: range2154550
version: 1
direction: direction/forward
top level: BBa_K567004
start: 278
end: 282
orientation: inline

luciferase-2AGG
type: SequenceAnnotation
persistent identity: annotation2150726
display id: annotation2150726
version: 1
top level: BBa_K567004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567004/annotation2150726/range2150726/1

https://synbiohub.org/public/igem/BBa_K567004/annotation2150726/range2150726/1
type: Range
persistent identity: range2150726
display id: range2150726
version: 1
direction: direction/forward
top level: BBa_K567004
start: 287
end: 1945
orientation: inline

2AGG
type: SequenceAnnotation
persistent identity: annotation2150725
display id: annotation2150725
version: 1
top level: BBa_K567004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567004/annotation2150725/range2150725/1

https://synbiohub.org/public/igem/BBa_K567004/annotation2150725/range2150725/1
type: Range
persistent identity: range2150725
display id: range2150725
version: 1
direction: direction/forward
top level: BBa_K567004
start: 290
end: 295
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150724
display id: annotation2150724
version: 1
top level: BBa_K567004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567004/annotation2150724/range2150724/1

https://synbiohub.org/public/igem/BBa_K567004/annotation2150724/range2150724/1
type: Range
persistent identity: range2150724
display id: range2150724
version: 1
direction: direction/forward
top level: BBa_K567004
start: 287
end: 289
orientation: inline

Pbla
type: SequenceAnnotation
persistent identity: annotation2152721
display id: annotation2152721
version: 1
top level: BBa_K567004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567004/annotation2152721/range2152721/1

https://synbiohub.org/public/igem/BBa_K567004/annotation2152721/range2152721/1
type: Range
persistent identity: range2152721
display id: range2152721
version: 1
direction: direction/forward
top level: BBa_K567004
start: 216
end: 244
orientation: inline

BBa_K1037001
type: ComponentDefinition
persistent identity: BBa K1037001
display id: BBa_K1037001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ygfD
creator: Zeng Jianhao
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1344_
m user id: 0
owner id: 11853
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: botin-independent propionyl-CoA carboxylase;a succinate (or propionate) CoA ligase, or a novel (biotin-independent) propionyl-CoA carboxylase
mutable notes: mutation of C to G to avoid illegal PstI site
mutable provenance: E.coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ygfD coding rigion
sequence annotation: TAA stop coden
sequence annotation: ATG start coden
sequence: https://synbiohub.org/public/igem/BBa_K1037001_sequence/1

ygfD coding rigion
type: SequenceAnnotation
persistent identity: annotation2337496
display id: annotation2337496
version: 1
top level: BBa_K1037001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1037001/annotation2337496/range2337496/1

https://synbiohub.org/public/igem/BBa_K1037001/annotation2337496/range2337496/1
type: Range
persistent identity: range2337496
display id: range2337496
version: 1
direction: direction/forward
top level: BBa_K1037001
start: 1
end: 996
orientation: inline

TAA stop coden
type: SequenceAnnotation
persistent identity: annotation2337497
display id: annotation2337497
version: 1
top level: BBa_K1037001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1037001/annotation2337497/range2337497/1

https://synbiohub.org/public/igem/BBa_K1037001/annotation2337497/range2337497/1
type: Range
persistent identity: range2337497
display id: range2337497
version: 1
direction: direction/forward
top level: BBa_K1037001
start: 994
end: 996
orientation: inline

ATG start coden
type: SequenceAnnotation
persistent identity: annotation2337495
display id: annotation2337495
version: 1
top level: BBa_K1037001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1037001/annotation2337495/range2337495/1

https://synbiohub.org/public/igem/BBa_K1037001/annotation2337495/range2337495/1
type: Range
persistent identity: range2337495
display id: range2337495
version: 1
direction: direction/forward
top level: BBa_K1037001
start: 1
end: 3
orientation: inline

ManA1
type: ComponentDefinition
persistent identity: BBa K1360003
display id: BBa_K1360003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: endo-1,4-beta-mannosidase ManA1
creator: Liang Jiang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1736_
m user id: 0
owner id: 22520
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: encodes endo-1,4-beta-xylanase by XynB from Clostridium stercorarium DSM8532, codon optimized for E.coli
mutable notes: codon optimized for E.coli
mutable provenance: whole genome sequencing of Clostridium stercorarium DSM8532
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: ManA1
sequence: https://synbiohub.org/public/igem/BBa_K1360003_sequence/1

ManA1
type: SequenceAnnotation
persistent identity: annotation2398056
display id: annotation2398056
version: 1
top level: ManA1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1360003/annotation2398056/range2398056/1

https://synbiohub.org/public/igem/BBa_K1360003/annotation2398056/range2398056/1
type: Range
persistent identity: range2398056
display id: range2398056
version: 1
direction: direction/forward
top level: ManA1
start: 1
end: 1530
orientation: inline

BBa_K1031625
type: ComponentDefinition
persistent identity: BBa K1031625
display id: BBa_K1031625
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K1031625_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361915
display id: annotation2361915
version: 1
top level: BBa_K1031625
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1
type: Range
persistent identity: range2361915
display id: range2361915
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361916
display id: annotation2361916
version: 1
top level: BBa_K1031625
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1
type: Range
persistent identity: range2361916
display id: range2361916
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1520
end: 1648
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2361913
display id: annotation2361913
version: 1
top level: BBa_K1031625
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1
type: Range
persistent identity: range2361913
display id: range2361913
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1
end: 35
orientation: inline

BBa_K1331001
type: ComponentDefinition
persistent identity: BBa K1331001
display id: BBa_K1331001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa.
mutable notes: A mutation has been done to remove the PstI restriction site contained in the coding sequence.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: rhlA
sequence: https://synbiohub.org/public/igem/BBa_K1331001_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427978
display id: annotation2427978
version: 1
top level: BBa_K1331001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1
type: Range
persistent identity: range2427978
display id: range2427978
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 889
end: 947
orientation: inline

rhlA
type: SequenceAnnotation
persistent identity: annotation2386609
display id: annotation2386609
version: 1
top level: BBa_K1331001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1
type: Range
persistent identity: range2386609
display id: range2386609
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 1
end: 888
orientation: inline

BBa_K389402
type: ComponentDefinition
persistent identity: BBa K389402
display id: BBa_K389402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified BBa_I746380 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part BBa_I746370
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_K389004
component: BBa_B0034
component: BBa_I746351
component: BBa_I746360
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K389004
sequence annotation: BBa_I746351
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_K389402_sequence/1

BBa_E1010
type: Component
persistent identity: component2273082
display id: component2273082
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K389004
type: Component
persistent identity: component2273096
display id: component2273096
version: 1
top level: BBa_K389402
access: public
definition: luc

BBa_B0034
type: Component
persistent identity: component2273093
display id: component2273093
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746351
type: Component
persistent identity: component2273077
display id: component2273077
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_I746360
type: Component
persistent identity: component2273091
display id: component2273091
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_B0015
type: Component
persistent identity: component2273089
display id: component2273089
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2273079
display id: component2273079
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273093
display id: annotation2273093
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273093/range2273093/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389402/annotation2273093/range2273093/1
type: Range
persistent identity: range2273093
display id: range2273093
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1214
end: 1225
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273096
display id: annotation2273096
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273096/range2273096/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389402/annotation2273096/range2273096/1
type: Range
persistent identity: range2273096
display id: range2273096
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1232
end: 2887
orientation: inline

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2273077
display id: annotation2273077
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273077/range2273077/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K389402/annotation2273077/range2273077/1
type: Range
persistent identity: range2273077
display id: range2273077
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1
end: 237
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273089
display id: annotation2273089
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273089/range2273089/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389402/annotation2273089/range2273089/1
type: Range
persistent identity: range2273089
display id: range2273089
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 978
end: 1106
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273079
display id: annotation2273079
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273079/range2273079/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389402/annotation2273079/range2273079/1
type: Range
persistent identity: range2273079
display id: range2273079
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 246
end: 257
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273082
display id: annotation2273082
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273082/range2273082/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389402/annotation2273082/range2273082/1
type: Range
persistent identity: range2273082
display id: range2273082
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 264
end: 969
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273091
display id: annotation2273091
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273091/range2273091/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389402/annotation2273091/range2273091/1
type: Range
persistent identity: range2273091
display id: range2273091
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1115
end: 1205
orientation: inline

BBa_M36013
type: ComponentDefinition
persistent identity: BBa M36013
display id: BBa_M36013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (alt1)
creator: Jesse Palmer, Elliot Lui
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase) and glgS gene (predicted glycogen synthesis protein). This part is greatly reduced in size from the ideal glycogen synthesis actuator by the removal of glgA.
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
mutable provenance: Composite of genomic sequences coding for proteins involved in e. coli glycogen biosynthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36009
component: BBa_M36724
component: BBa_M36010
component: BBa_M36008
component: BBa_M36006
sequence annotation: BBa_M36008
sequence annotation: BBa_M36006
sequence annotation: BBa_M36010
sequence annotation: BBa_M36009
sequence annotation: BBa_M36724
sequence: https://synbiohub.org/public/igem/BBa_M36013_sequence/1

BBa_M36009
type: Component
persistent identity: component2118539
display id: component2118539
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36724
type: Component
persistent identity: component2118541
display id: component2118541
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36010
type: Component
persistent identity: component2118543
display id: component2118543
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36008
type: Component
persistent identity: component2118542
display id: component2118542
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36008/1

BBa_M36006
type: Component
persistent identity: component2118540
display id: component2118540
version: 1
top level: BBa_M36013
access: public
definition: BBa_M36006

BBa_M36008
type: SequenceAnnotation
persistent identity: annotation2118542
display id: annotation2118542
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118542/range2118542/1
component: BBa_M36008

https://synbiohub.org/public/igem/BBa_M36013/annotation2118542/range2118542/1
type: Range
persistent identity: range2118542
display id: range2118542
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1429
end: 1629
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118540
display id: annotation2118540
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118540/range2118540/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36013/annotation2118540/range2118540/1
type: Range
persistent identity: range2118540
display id: range2118540
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 48
end: 1343
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118543
display id: annotation2118543
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118543/range2118543/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36013/annotation2118543/range2118543/1
type: Range
persistent identity: range2118543
display id: range2118543
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1630
end: 1711
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118539
display id: annotation2118539
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118539/range2118539/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36013/annotation2118539/range2118539/1
type: Range
persistent identity: range2118539
display id: range2118539
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1
end: 47
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118541
display id: annotation2118541
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118541/range2118541/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36013/annotation2118541/range2118541/1
type: Range
persistent identity: range2118541
display id: range2118541
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1344
end: 1428
orientation: inline

SgsE | Red
type: ComponentDefinition
persistent identity: BBa K525312
display id: BBa_K525312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and Oxidoreductase
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and Oxidoreductase;
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K525499
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: K525301
sequence annotation: Reductase
sequence annotation: SgsE
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: Freiburg scar
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: K525499
sequence: https://synbiohub.org/public/igem/BBa_K525312_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2136759
display id: annotation2136759
version: 1
top level: SgsE | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136759/range2136759/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136759/range2136759/1
type: Range
persistent identity: range2136759
display id: range2136759
version: 1
direction: direction/forward
top level: SgsE | Red
start: 76
end: 81
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2136758
display id: annotation2136758
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136758/range2136758/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136758/range2136758/1
type: Range
persistent identity: range2136758
display id: range2136758
version: 1
direction: direction/forward
top level: SgsE | Red
start: 73
end: 2400
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2136762
display id: annotation2136762
version: 1
top level: SgsE | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136762/range2136762/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136762/range2136762/1
type: Range
persistent identity: range2136762
display id: range2136762
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2407
end: 3156
orientation: inline

SgsE
type: SequenceAnnotation
persistent identity: annotation2136757
display id: annotation2136757
version: 1
top level: SgsE | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136757/range2136757/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136757/range2136757/1
type: Range
persistent identity: range2136757
display id: range2136757
version: 1
direction: direction/forward
top level: SgsE | Red
start: 73
end: 2400
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2136763
display id: annotation2136763
version: 1
top level: SgsE | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136763/range2136763/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136763/range2136763/1
type: Range
persistent identity: range2136763
display id: range2136763
version: 1
direction: direction/forward
top level: SgsE | Red
start: 3148
end: 3152
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2136755
display id: annotation2136755
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136755/range2136755/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136755/range2136755/1
type: Range
persistent identity: range2136755
display id: range2136755
version: 1
direction: direction/forward
top level: SgsE | Red
start: 53
end: 64
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136756
display id: annotation2136756
version: 1
top level: SgsE | Red
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136756/range2136756/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136756/range2136756/1
type: Range
persistent identity: range2136756
display id: range2136756
version: 1
direction: direction/forward
top level: SgsE | Red
start: 56
end: 59
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2136760
display id: annotation2136760
version: 1
top level: SgsE | Red
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136760/range2136760/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136760/range2136760/1
type: Range
persistent identity: range2136760
display id: range2136760
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2401
end: 2406
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2136753
display id: annotation2136753
version: 1
top level: SgsE | Red
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136753/range2136753/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136753/range2136753/1
type: Range
persistent identity: range2136753
display id: range2136753
version: 1
direction: direction/forward
top level: SgsE | Red
start: 1
end: 20
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2136754
display id: annotation2136754
version: 1
top level: SgsE | Red
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136754/range2136754/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136754/range2136754/1
type: Range
persistent identity: range2136754
display id: range2136754
version: 1
direction: direction/forward
top level: SgsE | Red
start: 20
end: 44
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2136761
display id: annotation2136761
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136761/range2136761/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136761/range2136761/1
type: Range
persistent identity: range2136761
display id: range2136761
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2407
end: 3156
orientation: inline

BBa_K1462020
type: ComponentDefinition
persistent identity: BBa K1462020
display id: BBa_K1462020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erg10
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462020_sequence/1

BBa_K1031620
type: ComponentDefinition
persistent identity: BBa K1031620
display id: BBa_K1031620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031620_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2360134
display id: annotation2360134
version: 1
top level: BBa_K1031620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1
type: Range
persistent identity: range2360134
display id: range2360134
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1
end: 1452
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360136
display id: annotation2360136
version: 1
top level: BBa_K1031620
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1
type: Range
persistent identity: range2360136
display id: range2360136
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1459
end: 1587
orientation: inline

BBa_K1342001
type: ComponentDefinition
persistent identity: BBa K1342001
display id: BBa_K1342001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zinTp_AspA
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is
mutable notes: soon
mutable provenance: it come from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: promoter
sequence annotation: cds
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1342001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2394010
display id: annotation2394010
version: 1
top level: BBa_K1342001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1
type: Range
persistent identity: range2394010
display id: range2394010
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 1
end: 81
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2394012
display id: annotation2394012
version: 1
top level: BBa_K1342001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1
type: Range
persistent identity: range2394012
display id: range2394012
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 108
end: 1589
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2394011
display id: annotation2394011
version: 1
top level: BBa_K1342001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1
type: Range
persistent identity: range2394011
display id: range2394011
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 82
end: 101
orientation: inline

BBa_K1072002
type: ComponentDefinition
persistent identity: BBa K1072002
display id: BBa_K1072002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH dehydrogenase
creator: Yichong wang, Ruichen, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072002 is the generator fo NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_K1072001
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K1072001
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1072002_sequence/1

BBa_B0010
type: Component
persistent identity: component2334353
display id: component2334353
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1072001
type: Component
persistent identity: component2334352
display id: component2334352
version: 1
top level: BBa_K1072002
access: public
definition: BBa_K1072001

BBa_R0062
type: Component
persistent identity: component2334344
display id: component2334344
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component2334350
display id: component2334350
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2334355
display id: component2334355
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334355
display id: annotation2334355
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
type: Range
persistent identity: range2334355
display id: range2334355
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1485
end: 1525
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334344
display id: annotation2334344
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
type: Range
persistent identity: range2334344
display id: range2334344
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334350
display id: annotation2334350
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
type: Range
persistent identity: range2334350
display id: range2334350
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 64
end: 75
orientation: inline

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2334352
display id: annotation2334352
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
component: BBa_K1072001

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
type: Range
persistent identity: range2334352
display id: range2334352
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 84
end: 1388
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334353
display id: annotation2334353
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
type: Range
persistent identity: range2334353
display id: range2334353
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1397
end: 1476
orientation: inline

KanR Backw
type: ComponentDefinition
persistent identity: BBa J31002
display id: BBa_J31002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance backwards (KanB) [cf. BBa_J23012 & BBa_J31003]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Codes the reverse of the Kanamycin Resistance gene. When the region is flipped, Kanamycin resistance will be expressed as long as a promoter and RBS are in front of the region.
mutable notes: None
mutable provenance: pSB1AK3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of KanR gene
sequence: https://synbiohub.org/public/igem/BBa_J31002_sequence/1

Reverse of KanR gene
type: SequenceAnnotation
persistent identity: annotation1884995
display id: annotation1884995
version: 1
top level: KanR Backw
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1

https://synbiohub.org/public/igem/BBa_J31002/annotation1884995/range1884995/1
type: Range
persistent identity: range1884995
display id: range1884995
version: 1
direction: direction/reverse
top level: KanR Backw
start: 1
end: 816
orientation: inline

BBa_K1199044
type: ComponentDefinition
persistent identity: BBa K1199044
display id: BBa_K1199044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HheCW249P 2,3-DCP(2,3-dichloropropanol)->Glycerol
creator: Tian Wang
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1513_
m user id: 0
owner id: 16962
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: HheC displayed high regioselectivity and moderate to high enantioselectivity that can be applied for the kinetic resolution of chiral spiroepoxides.And HheCW249P is mutant of HheC ,displayed higher activity than the wide type .
mutable notes: No restriction site contained
mutable provenance: HheC is from Agrobacterium radiobacter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1199044_sequence/1

ACCoxidase
type: ComponentDefinition
persistent identity: BBa K417002
display id: BBa_K417002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1-aminocyclopropane-1-carboxylate (ACC) oxidase
creator: Ben Porebski, Andrew Perry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, which catalyses the formation of ethylene from 1-aminocyclopropane-1-carboxylate under the presence of ascorbate and O2. (1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O) This enzyme is commonly referred to as ACC oxidase or ethylene forming enzyme (EFE).
mutable notes: Codon optimization for E. coli and removal of biobrick restriction sites.
mutable provenance: Solanum lycopersicum (Tomato) (Lycopersicon esculentum) codon optimized for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: ACC Oxidase
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K417002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2090378
display id: annotation2090378
version: 1
top level: ACCoxidase
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1
type: Range
persistent identity: range2090378
display id: range2090378
version: 1
direction: direction/forward
top level: ACCoxidase
start: 1
end: 3
orientation: inline

ACC Oxidase
type: SequenceAnnotation
persistent identity: annotation2090379
display id: annotation2090379
version: 1
top level: ACCoxidase
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1
type: Range
persistent identity: range2090379
display id: range2090379
version: 1
direction: direction/forward
top level: ACCoxidase
start: 1
end: 948
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2090380
display id: annotation2090380
version: 1
top level: ACCoxidase
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1
type: Range
persistent identity: range2090380
display id: range2090380
version: 1
direction: direction/forward
top level: ACCoxidase
start: 946
end: 948
orientation: inline

BBa_J52011
type: ComponentDefinition
persistent identity: BBa J52011
display id: BBa_J52011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: dnMyD88-linker-Rluc
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52011_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902834
display id: annotation1902834
version: 1
top level: BBa_J52011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1
type: Range
persistent identity: range1902834
display id: range1902834
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902835
display id: annotation1902835
version: 1
top level: BBa_J52011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1
type: Range
persistent identity: range1902835
display id: range1902835
version: 1
direction: direction/forward
top level: BBa_J52011
start: 418
end: 435
orientation: inline

dnMyD88-linker-Rluc
type: SequenceAnnotation
persistent identity: annotation1902846
display id: annotation1902846
version: 1
top level: BBa_J52011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1
type: Range
persistent identity: range1902846
display id: range1902846
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 1371
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902836
display id: annotation1902836
version: 1
top level: BBa_J52011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1
type: Range
persistent identity: range1902836
display id: range1902836
version: 1
direction: direction/forward
top level: BBa_J52011
start: 436
end: 1371
orientation: inline

BBa_Y00100
type: ComponentDefinition
persistent identity: BBa Y00100
display id: BBa_Y00100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nuclear 5-aminolevulinate synthase gene from S. cerevisiae, recoded for mitochondrial expression
creator: Natalie Kuldell
created: 2006-08-03T11:00:00Z
modified: 2015-05-08T01:14:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _43_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The first step in S. cerevisiae's pathway for heme biosynthesis is catalyzed by the product of the HEM1 gene [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=HEM1]. HEM1 is a nuclear gene (1.647 kb,[http://db.yeastgenome.org/cgi-bin/getSeq?seq=YDR232W&flankl=0&flankr=0&map=a3map]) that encodes the enzyme 5-aminolevulinate ("ALA" or "d-ALA") synthase (549 aa,[http://db.yeastgenome.org/cgi-bin/getSeq?seq=YDR232W&flankl=0&flankr=0&map=p3map]). The enzyme is post-translationally imported into the mitochondria where it is responsible for converting glycine and succinyl-CoA to 5-aminolevulinate ("d-ALA") plus CoA and CO2. Deletion of HEM1 is lethal for the cell but viabilty can be restored by providing the cells with the product of the HEM1p catalyzed reaction, namely d-ALA. In an effort to directly express the HEM1 gene from the mitochondrial genome, the HEM1 gene was recoded by DNA 2.0 for mitochondrial codons, an epitope tag (HA = YPYDVPDYA) was added to the C-terminal portion of the protein just before the stop codon, and flanking sequences (100 bp upstm of ATG and 92 dwstm of stop) were added to facilitate integration into mitochondrial genome.
mutable notes: codon optimized for mitochondrial gene expression, HA-epitope C term (not N-term where mt import sequences rest), flank coding seq with homology to COX3 gene of mitochondria to allow for integration and disruption of this gene (should make respiration+ cells become respiration-), and finally added BB prefix and suffix to allow cloning into std Registry vectors
mutable provenance: direct synthesis from DNA 2.0, summer 2006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: for recombination to COX3
sequence annotation: coding sequence
sequence annotation: HA
sequence annotation: TGA stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_Y00100_sequence/1

for recombination to COX3
type: SequenceAnnotation
persistent identity: annotation1975601
display id: annotation1975601
version: 1
top level: BBa_Y00100
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975601/range1975601/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975601/range1975601/1
type: Range
persistent identity: range1975601
display id: range1975601
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1
end: 101
orientation: inline

coding sequence
type: SequenceAnnotation
persistent identity: annotation1975606
display id: annotation1975606
version: 1
top level: BBa_Y00100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975606/range1975606/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975606/range1975606/1
type: Range
persistent identity: range1975606
display id: range1975606
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 101
end: 1774
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation1975602
display id: annotation1975602
version: 1
top level: BBa_Y00100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975602/range1975602/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975602/range1975602/1
type: Range
persistent identity: range1975602
display id: range1975602
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1745
end: 1771
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation1975603
display id: annotation1975603
version: 1
top level: BBa_Y00100
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975603/range1975603/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975603/range1975603/1
type: Range
persistent identity: range1975603
display id: range1975603
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1772
end: 1774
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1975605
display id: annotation1975605
version: 1
top level: BBa_Y00100
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975605/range1975605/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975605/range1975605/1
type: Range
persistent identity: range1975605
display id: range1975605
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 101
end: 103
orientation: inline

for recombination to COX3
type: SequenceAnnotation
persistent identity: annotation1975604
display id: annotation1975604
version: 1
top level: BBa_Y00100
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_Y00100/annotation1975604/range1975604/1

https://synbiohub.org/public/igem/BBa_Y00100/annotation1975604/range1975604/1
type: Range
persistent identity: range1975604
display id: range1975604
version: 1
direction: direction/forward
top level: BBa_Y00100
start: 1775
end: 1865
orientation: inline

cyaA
type: ComponentDefinition
persistent identity: BBa K845008
display id: BBa_K845008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyaA with its natural RBS
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: WT RBS
sequence annotation: cyaA
sequence: https://synbiohub.org/public/igem/BBa_K845008_sequence/1

WT RBS
type: SequenceAnnotation
persistent identity: annotation2203949
display id: annotation2203949
version: 1
top level: cyaA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K845008/annotation2203949/range2203949/1

https://synbiohub.org/public/igem/BBa_K845008/annotation2203949/range2203949/1
type: Range
persistent identity: range2203949
display id: range2203949
version: 1
direction: direction/forward
top level: cyaA
start: 1
end: 30
orientation: inline

cyaA
type: SequenceAnnotation
persistent identity: annotation2203950
display id: annotation2203950
version: 1
top level: cyaA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K845008/annotation2203950/range2203950/1

https://synbiohub.org/public/igem/BBa_K845008/annotation2203950/range2203950/1
type: Range
persistent identity: range2203950
display id: range2203950
version: 1
direction: direction/forward
top level: cyaA
start: 30
end: 2577
orientation: inline

BBa_K389302
type: ComponentDefinition
persistent identity: BBa K389302
display id: BBa_K389302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a weak constitutive promoter.
mutable notes: weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23103
component: BBa_K389004
component: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389302_sequence/1

BBa_J23103
type: Component
persistent identity: component2085573
display id: component2085573
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K389004
type: Component
persistent identity: component2085578
display id: component2085578
version: 1
top level: BBa_K389302
access: public
definition: luc

BBa_B0034
type: Component
persistent identity: component2085575
display id: component2085575
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2085573
display id: annotation2085573
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
type: Range
persistent identity: range2085573
display id: range2085573
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085578
display id: annotation2085578
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
type: Range
persistent identity: range2085578
display id: range2085578
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085575
display id: annotation2085575
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
type: Range
persistent identity: range2085575
display id: range2085575
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 44
end: 55
orientation: inline

LipA
type: ComponentDefinition
persistent identity: BBa K836000
display id: BBa_K836000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lipA from B. cepacia (codon usage optimized for E. coli)
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit).
mutable notes: The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.
mutable provenance: The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lipA
sequence: https://synbiohub.org/public/igem/BBa_K836000_sequence/1

lipA
type: SequenceAnnotation
persistent identity: annotation2185259
display id: annotation2185259
version: 1
top level: LipA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836000/annotation2185259/range2185259/1

https://synbiohub.org/public/igem/BBa_K836000/annotation2185259/range2185259/1
type: Range
persistent identity: range2185259
display id: range2185259
version: 1
direction: direction/forward
top level: LipA
start: 1
end: 1098
orientation: inline

BBa_K2185005
type: ComponentDefinition
persistent identity: BBa K2185005
display id: BBa_K2185005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B.cereus beta amylase w/ no promoter
creator: Marissa Sumathipala
created: 2016-09-04T11:00:00Z
modified: 2016-09-15T01:58:44Z
discontinued: false
dominant: false
group u list: _2661_
m user id: 27970
owner id: 27970
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the bacterium B.cereus that codes for a beta amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36).
mutable notes: Codon optimized for S.cerevisiae, Kpn and Spe sites removed.
mutable provenance: It is a composite part, coding sequence is sourced from the B.cereus genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2185004
component: BBa_K165002
component: BBa_K792002
component: BBa_K392003
sequence annotation: BBa_K392003
sequence annotation: BBa_K792002
sequence annotation: BBa_K2185004
sequence annotation: BBa_K165002
sequence: https://synbiohub.org/public/igem/BBa_K2185005_sequence/1

BBa_K2185004
type: Component
persistent identity: component2483549
display id: component2483549
version: 1
top level: BBa_K2185005
access: public
definition: https://synbiohub.org/public/igem/BBa_K2185004/1

BBa_K165002
type: Component
persistent identity: component2483546
display id: component2483546
version: 1
top level: BBa_K2185005
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K792002
type: Component
persistent identity: component2483548
display id: component2483548
version: 1
top level: BBa_K2185005
access: public
definition: https://synbiohub.org/public/igem/BBa_K792002/1

BBa_K392003
type: Component
persistent identity: component2483550
display id: component2483550
version: 1
top level: BBa_K2185005
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2483550
display id: annotation2483550
version: 1
top level: BBa_K2185005
location: https://synbiohub.org/public/igem/BBa_K2185005/annotation2483550/range2483550/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K2185005/annotation2483550/range2483550/1
type: Range
persistent identity: range2483550
display id: range2483550
version: 1
direction: direction/reverse
top level: BBa_K2185005
start: 1736
end: 1864
orientation: inline

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2483548
display id: annotation2483548
version: 1
top level: BBa_K2185005
location: https://synbiohub.org/public/igem/BBa_K2185005/annotation2483548/range2483548/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K2185005/annotation2483548/range2483548/1
type: Range
persistent identity: range2483548
display id: range2483548
version: 1
direction: direction/reverse
top level: BBa_K2185005
start: 27
end: 80
orientation: inline

BBa_K2185004
type: SequenceAnnotation
persistent identity: annotation2483549
display id: annotation2483549
version: 1
top level: BBa_K2185005
location: https://synbiohub.org/public/igem/BBa_K2185005/annotation2483549/range2483549/1
component: BBa_K2185004

https://synbiohub.org/public/igem/BBa_K2185005/annotation2483549/range2483549/1
type: Range
persistent identity: range2483549
display id: range2483549
version: 1
direction: direction/reverse
top level: BBa_K2185005
start: 87
end: 1727
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2483546
display id: annotation2483546
version: 1
top level: BBa_K2185005
location: https://synbiohub.org/public/igem/BBa_K2185005/annotation2483546/range2483546/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K2185005/annotation2483546/range2483546/1
type: Range
persistent identity: range2483546
display id: range2483546
version: 1
direction: direction/reverse
top level: BBa_K2185005
start: 1
end: 18
orientation: inline

BBa_K552003
type: ComponentDefinition
persistent identity: BBa K552003
display id: BBa_K552003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtI Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ADH1 Terminator
sequence annotation: Stop Codon
sequence annotation: PGK Promoter
sequence annotation: Start Codon
sequence annotation: CrtI
sequence: https://synbiohub.org/public/igem/BBa_K552003_sequence/1

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145134
display id: annotation2145134
version: 1
top level: BBa_K552003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145134/range2145134/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145134/range2145134/1
type: Range
persistent identity: range2145134
display id: range2145134
version: 1
direction: direction/forward
top level: BBa_K552003
start: 2130
end: 2258
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145133
display id: annotation2145133
version: 1
top level: BBa_K552003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145133/range2145133/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145133/range2145133/1
type: Range
persistent identity: range2145133
display id: range2145133
version: 1
direction: direction/forward
top level: BBa_K552003
start: 2127
end: 2129
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145122
display id: annotation2145122
version: 1
top level: BBa_K552003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145122/range2145122/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145122/range2145122/1
type: Range
persistent identity: range2145122
display id: range2145122
version: 1
direction: direction/forward
top level: BBa_K552003
start: 1
end: 380
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145131
display id: annotation2145131
version: 1
top level: BBa_K552003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145131/range2145131/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145131/range2145131/1
type: Range
persistent identity: range2145131
display id: range2145131
version: 1
direction: direction/forward
top level: BBa_K552003
start: 381
end: 383
orientation: inline

CrtI
type: SequenceAnnotation
persistent identity: annotation2145132
display id: annotation2145132
version: 1
top level: BBa_K552003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145132/range2145132/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145132/range2145132/1
type: Range
persistent identity: range2145132
display id: range2145132
version: 1
direction: direction/forward
top level: BBa_K552003
start: 381
end: 2129
orientation: inline

BBa_K552001
type: ComponentDefinition
persistent identity: BBa K552001
display id: BBa_K552001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtE Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-23T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the enzyme CrtE, also known as geranylgeranyl diphosphate synthase. CrtE serves as the first step in carotenoid biosynthesis in Saccharomyces cerevisiae by converting farnesyl diphosphate (which is naturally found in yeast) to geranylgeranyl diphosphate.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ADH1 Terminator
sequence annotation: Stop Codon
sequence annotation: Start Codon
sequence annotation: PGK Promoter
sequence annotation: CrtE
sequence: https://synbiohub.org/public/igem/BBa_K552001_sequence/1

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2139162
display id: annotation2139162
version: 1
top level: BBa_K552001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139162/range2139162/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139162/range2139162/1
type: Range
persistent identity: range2139162
display id: range2139162
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1512
end: 1639
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2139161
display id: annotation2139161
version: 1
top level: BBa_K552001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139161/range2139161/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139161/range2139161/1
type: Range
persistent identity: range2139161
display id: range2139161
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1508
end: 1510
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2139159
display id: annotation2139159
version: 1
top level: BBa_K552001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139159/range2139159/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139159/range2139159/1
type: Range
persistent identity: range2139159
display id: range2139159
version: 1
direction: direction/forward
top level: BBa_K552001
start: 381
end: 383
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2139158
display id: annotation2139158
version: 1
top level: BBa_K552001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139158/range2139158/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139158/range2139158/1
type: Range
persistent identity: range2139158
display id: range2139158
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1
end: 380
orientation: inline

CrtE
type: SequenceAnnotation
persistent identity: annotation2139160
display id: annotation2139160
version: 1
top level: BBa_K552001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139160/range2139160/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139160/range2139160/1
type: Range
persistent identity: range2139160
display id: range2139160
version: 1
direction: direction/forward
top level: BBa_K552001
start: 381
end: 1511
orientation: inline

BBa_K1679006
type: ComponentDefinition
persistent identity: BBa K1679006
display id: BBa_K1679006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RiboJ and GFP
creator: Qikai Qin
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T08:44:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part is a composite of RiboJ and I13504 which can be used to express GFP if added a promoter.
mutable notes: In order to make other team easily use a GFP part to test the strength of a promoter, we constructed this part contain RiboJ before the BBa_I13504. Any team can use this part to measure the GFP expression if added a promoter.
mutable provenance: RiboJ BBa_I13504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1679038
component: BBa_I13504
sequence annotation: BBa_I13504
sequence annotation: BBa_K1679038
sequence: https://synbiohub.org/public/igem/BBa_K1679006_sequence/1

BBa_K1679038
type: Component
persistent identity: component2474727
display id: component2474727
version: 1
top level: BBa_K1679006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1679038/1

BBa_I13504
type: Component
persistent identity: component2474737
display id: component2474737
version: 1
top level: BBa_K1679006
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2474737
display id: annotation2474737
version: 1
top level: BBa_K1679006
location: https://synbiohub.org/public/igem/BBa_K1679006/annotation2474737/range2474737/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679006/annotation2474737/range2474737/1
type: Range
persistent identity: range2474737
display id: range2474737
version: 1
direction: direction/reverse
top level: BBa_K1679006
start: 84
end: 958
orientation: inline

BBa_K1679038
type: SequenceAnnotation
persistent identity: annotation2474727
display id: annotation2474727
version: 1
top level: BBa_K1679006
location: https://synbiohub.org/public/igem/BBa_K1679006/annotation2474727/range2474727/1
component: BBa_K1679038

https://synbiohub.org/public/igem/BBa_K1679006/annotation2474727/range2474727/1
type: Range
persistent identity: range2474727
display id: range2474727
version: 1
direction: direction/reverse
top level: BBa_K1679006
start: 1
end: 75
orientation: inline

BBa_K1331004
type: ComponentDefinition
persistent identity: BBa K1331004
display id: BBa_K1331004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable notes: Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable provenance: Pseudomonas aeruginosa SQ6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: RhlB
sequence: https://synbiohub.org/public/igem/BBa_K1331004_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434210
display id: annotation2434210
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1
type: Range
persistent identity: range2434210
display id: range2434210
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1288
end: 1307
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434208
display id: annotation2434208
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1
type: Range
persistent identity: range2434208
display id: range2434208
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1
end: 6
orientation: inline

RhlB
type: SequenceAnnotation
persistent identity: annotation2434209
display id: annotation2434209
version: 1
top level: BBa_K1331004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1
type: Range
persistent identity: range2434209
display id: range2434209
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 7
end: 1287
orientation: inline

spxB
type: ComponentDefinition
persistent identity: BBa K137069
display id: BBa_K137069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pyruvate oxidase (SpxB)
creator: Doug Tischer
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the pyruvate oxidase from Streptococcus pneumoniae R6. It catalyzes the formation of hydrogen peroxide by oxidizing pyruvate in the reaction: pyruvate + Pi + O2 --> acetyl-P + CO2 + H2O2.
mutable notes: Removal of a PstI site very near the 5' end with PCR primers.
mutable provenance: Genomic PCR from a prepared genome of Streptococcus pneumoniae R6 from ATCC (# BAA-255D-5)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T->G
sequence: https://synbiohub.org/public/igem/BBa_K137069_sequence/1

T->G
type: SequenceAnnotation
persistent identity: annotation1969567
display id: annotation1969567
version: 1
top level: spxB
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137069/annotation1969567/range1969567/1

https://synbiohub.org/public/igem/BBa_K137069/annotation1969567/range1969567/1
type: Range
persistent identity: range1969567
display id: range1969567
version: 1
direction: direction/forward
top level: spxB
start: 27
end: 27
orientation: inline

BBa_K1031922
type: ComponentDefinition
persistent identity: BBa K1031922
display id: BBa_K1031922
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23114-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylB
sequence annotation: J23114
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031922_sequence/1

XylB
type: SequenceAnnotation
persistent identity: annotation2370037
display id: annotation2370037
version: 1
top level: BBa_K1031922
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1
type: Range
persistent identity: range2370037
display id: range2370037
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 63
end: 1169
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2370036
display id: annotation2370036
version: 1
top level: BBa_K1031922
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1
type: Range
persistent identity: range2370036
display id: range2370036
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370038
display id: annotation2370038
version: 1
top level: BBa_K1031922
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1
type: Range
persistent identity: range2370038
display id: range2370038
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1176
end: 1304
orientation: inline

BBa_K1072006
type: ComponentDefinition
persistent identity: BBa K1072006
display id: BBa_K1072006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pR+RBS+α-ALS+2TM
creator: Ruichen, Yichong wang and Junjie zhang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: α-ALS gene placed under the control of Lux pR promoter. After co-transforming with BBa_K1072000 into E.coli cells,the luxR and AHL complex will activate the Lux pR promotor and transcript α-ALS and GFP genes.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_B0012
component: BBa_B0034
component: BBa_K1072004
component: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K1072004
sequence: https://synbiohub.org/public/igem/BBa_K1072006_sequence/1

BBa_R0062
type: Component
persistent identity: component2352688
display id: component2352688
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2352699
display id: component2352699
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2352694
display id: component2352694
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1072004
type: Component
persistent identity: component2352696
display id: component2352696
version: 1
top level: BBa_K1072006
access: public
definition: BBa_K1072004

BBa_B0010
type: Component
persistent identity: component2352697
display id: component2352697
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2352688
display id: annotation2352688
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352688/range2352688/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352688/range2352688/1
type: Range
persistent identity: range2352688
display id: range2352688
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2352697
display id: annotation2352697
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352697/range2352697/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352697/range2352697/1
type: Range
persistent identity: range2352697
display id: range2352697
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1755
end: 1834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2352694
display id: annotation2352694
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352694/range2352694/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352694/range2352694/1
type: Range
persistent identity: range2352694
display id: range2352694
version: 1
direction: direction/reverse
top level: BBa_K1072006
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2352699
display id: annotation2352699
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352699/range2352699/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352699/range2352699/1
type: Range
persistent identity: range2352699
display id: range2352699
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1843
end: 1883
orientation: inline

BBa_K1072004
type: SequenceAnnotation
persistent identity: annotation2352696
display id: annotation2352696
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352696/range2352696/1
component: BBa_K1072004

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352696/range2352696/1
type: Range
persistent identity: range2352696
display id: range2352696
version: 1
direction: direction/reverse
top level: BBa_K1072006
start: 82
end: 1746
orientation: inline

BBa_K1709001
type: ComponentDefinition
persistent identity: BBa K1709001
display id: BBa_K1709001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxI-His
creator: Astrid Deryckere
created: 2015-09-07T11:00:00Z
modified: 2015-09-26T09:17:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25069
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1709000
component: BBa_J61104
sequence annotation: BBa_J61104
sequence annotation: BBa_K1709000
sequence: https://synbiohub.org/public/igem/BBa_K1709001_sequence/1

BBa_K1709000
type: Component
persistent identity: component2473568
display id: component2473568
version: 1
top level: BBa_K1709001
access: public
definition: LuxI-His

BBa_J61104
type: Component
persistent identity: component2473563
display id: component2473563
version: 1
top level: BBa_K1709001
access: public
definition: https://synbiohub.org/public/igem/BBa_J61104/1

BBa_J61104
type: SequenceAnnotation
persistent identity: annotation2473563
display id: annotation2473563
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473563/range2473563/1
component: BBa_J61104

https://synbiohub.org/public/igem/BBa_K1709001/annotation2473563/range2473563/1
type: Range
persistent identity: range2473563
display id: range2473563
version: 1
direction: direction/reverse
top level: BBa_K1709001
start: 1
end: 12
orientation: inline

BBa_K1709000
type: SequenceAnnotation
persistent identity: annotation2473568
display id: annotation2473568
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473568/range2473568/1
component: BBa_K1709000

https://synbiohub.org/public/igem/BBa_K1709001/annotation2473568/range2473568/1
type: Range
persistent identity: range2473568
display id: range2473568
version: 1
direction: direction/reverse
top level: BBa_K1709001
start: 19
end: 627
orientation: inline

BBa_J52012
type: ComponentDefinition
persistent identity: BBa J52012
display id: BBa_J52012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rluc-linker-PEST191 is a part composed of Rluc (which acts as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence.
mutable notes: Rluc-linker-PEST 191 was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Rluc
sequence annotation: PEST191
sequence annotation: Rluc-linker-PEST191
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_J52012_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902837
display id: annotation1902837
version: 1
top level: BBa_J52012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902837/range1902837/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902837/range1902837/1
type: Range
persistent identity: range1902837
display id: range1902837
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 933
orientation: inline

PEST191
type: SequenceAnnotation
persistent identity: annotation1902839
display id: annotation1902839
version: 1
top level: BBa_J52012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1
type: Range
persistent identity: range1902839
display id: range1902839
version: 1
direction: direction/forward
top level: BBa_J52012
start: 952
end: 1437
orientation: inline

Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902847
display id: annotation1902847
version: 1
top level: BBa_J52012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1
type: Range
persistent identity: range1902847
display id: range1902847
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 1437
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902838
display id: annotation1902838
version: 1
top level: BBa_J52012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1
type: Range
persistent identity: range1902838
display id: range1902838
version: 1
direction: direction/forward
top level: BBa_J52012
start: 934
end: 951
orientation: inline

BBa_K2127003
type: ComponentDefinition
persistent identity: BBa K2127003
display id: BBa_K2127003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psbT
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-25T05:51:22Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 32415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: psbT
mutable notes: coding
mutable provenance: synchocystis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K2127007
component: BBa_R0010
component: BBa_B0032
component: BBa_K2127005
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127005
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K2127003_sequence/1

BBa_K2127007
type: Component
persistent identity: component2526862
display id: component2526862
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_R0010
type: Component
persistent identity: component2526848
display id: component2526848
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0032
type: Component
persistent identity: component2526856
display id: component2526856
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K2127005
type: Component
persistent identity: component2526861
display id: component2526861
version: 1
top level: BBa_K2127003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127005/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2526848
display id: annotation2526848
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526848/range2526848/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526848/range2526848/1
type: Range
persistent identity: range2526848
display id: range2526848
version: 1
direction: direction/forward
top level: BBa_K2127003
start: 1
end: 200
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2526862
display id: annotation2526862
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526862/range2526862/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526862/range2526862/1
type: Range
persistent identity: range2526862
display id: range2526862
version: 1
direction: direction/reverse
top level: BBa_K2127003
start: 433
end: 555
orientation: inline

BBa_K2127005
type: SequenceAnnotation
persistent identity: annotation2526861
display id: annotation2526861
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526861/range2526861/1
component: BBa_K2127005

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526861/range2526861/1
type: Range
persistent identity: range2526861
display id: range2526861
version: 1
direction: direction/reverse
top level: BBa_K2127003
start: 214
end: 432
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2526856
display id: annotation2526856
version: 1
top level: BBa_K2127003
location: https://synbiohub.org/public/igem/BBa_K2127003/annotation2526856/range2526856/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2127003/annotation2526856/range2526856/1
type: Range
persistent identity: range2526856
display id: range2526856
version: 1
direction: direction/forward
top level: BBa_K2127003
start: 201
end: 213
orientation: inline

BBa_K567015
type: ComponentDefinition
persistent identity: BBa K567015
display id: BBa_K567015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-metGN
creator: You Wang
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate.
mutable notes: overlapping PCR is used
mutable provenance: metG from E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: MetRS(truncated)
sequence annotation: T7 TE
sequence annotation: rbs
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K567015_sequence/1

MetRS(truncated)
type: SequenceAnnotation
persistent identity: annotation2153247
display id: annotation2153247
version: 1
top level: BBa_K567015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153247/range2153247/1
type: Range
persistent identity: range2153247
display id: range2153247
version: 1
direction: direction/forward
top level: BBa_K567015
start: 89
end: 1219
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2153277
display id: annotation2153277
version: 1
top level: BBa_K567015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2153277/range2153277/1
type: Range
persistent identity: range2153277
display id: range2153277
version: 1
direction: direction/forward
top level: BBa_K567015
start: 1389
end: 1436
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150831
display id: annotation2150831
version: 1
top level: BBa_K567015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150831/range2150831/1
type: Range
persistent identity: range2150831
display id: range2150831
version: 1
direction: direction/forward
top level: BBa_K567015
start: 75
end: 81
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150829
display id: annotation2150829
version: 1
top level: BBa_K567015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150829/range2150829/1
type: Range
persistent identity: range2150829
display id: range2150829
version: 1
direction: direction/forward
top level: BBa_K567015
start: 2
end: 18
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150830
display id: annotation2150830
version: 1
top level: BBa_K567015
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1

https://synbiohub.org/public/igem/BBa_K567015/annotation2150830/range2150830/1
type: Range
persistent identity: range2150830
display id: range2150830
version: 1
direction: direction/forward
top level: BBa_K567015
start: 21
end: 44
orientation: inline

BBa_K567011
type: ComponentDefinition
persistent identity: BBa K567011
display id: BBa_K567011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-TDRS
creator: Bin Zhao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNA(Asp)-TAG (BBa_K567013) and tRNA(Asp)-AGG (BBa_K567012).
mutable notes: Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS.
mutable provenance: aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: T7 promoter
sequence annotation: AspRS(truncated)
sequence annotation: T7 TE
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K567011_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2150825
display id: annotation2150825
version: 1
top level: BBa_K567011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567011/annotation2150825/range2150825/1

https://synbiohub.org/public/igem/BBa_K567011/annotation2150825/range2150825/1
type: Range
persistent identity: range2150825
display id: range2150825
version: 1
direction: direction/forward
top level: BBa_K567011
start: 75
end: 81
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150824
display id: annotation2150824
version: 1
top level: BBa_K567011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567011/annotation2150824/range2150824/1

https://synbiohub.org/public/igem/BBa_K567011/annotation2150824/range2150824/1
type: Range
persistent identity: range2150824
display id: range2150824
version: 1
direction: direction/forward
top level: BBa_K567011
start: 2
end: 18
orientation: inline

AspRS(truncated)
type: SequenceAnnotation
persistent identity: annotation2152822
display id: annotation2152822
version: 1
top level: BBa_K567011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567011/annotation2152822/range2152822/1

https://synbiohub.org/public/igem/BBa_K567011/annotation2152822/range2152822/1
type: Range
persistent identity: range2152822
display id: range2152822
version: 1
direction: direction/forward
top level: BBa_K567011
start: 89
end: 1549
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152823
display id: annotation2152823
version: 1
top level: BBa_K567011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567011/annotation2152823/range2152823/1

https://synbiohub.org/public/igem/BBa_K567011/annotation2152823/range2152823/1
type: Range
persistent identity: range2152823
display id: range2152823
version: 1
direction: direction/forward
top level: BBa_K567011
start: 1719
end: 1766
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150810
display id: annotation2150810
version: 1
top level: BBa_K567011
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567011/annotation2150810/range2150810/1

https://synbiohub.org/public/igem/BBa_K567011/annotation2150810/range2150810/1
type: Range
persistent identity: range2150810
display id: range2150810
version: 1
direction: direction/forward
top level: BBa_K567011
start: 21
end: 44
orientation: inline

BBa_K1666004
type: ComponentDefinition
persistent identity: BBa K1666004
display id: BBa_K1666004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrK of LuxS/AI-2 signaling pathway in Salmonalla
creator: Heming Wang
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T09:14:01Z
discontinued: false
dominant: false
group u list: _2084_
m user id: 28478
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And LsrK is the kinase which catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2. In our project, we set this protein-coding part under a nisA promoter and try to integrate them in the genome of Lactobacillus or Lactococcus for the final purpose of constructing an integrated AI-2 response pathway of Salmonella in the engineered bacteria.
mutable notes: The lsrK gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the gene LsrK(Gene ID: 1255599).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lsrK
sequence: https://synbiohub.org/public/igem/BBa_K1666004_sequence/1

lsrK
type: SequenceAnnotation
persistent identity: annotation2445114
display id: annotation2445114
version: 1
top level: BBa_K1666004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1666004/annotation2445114/range2445114/1

https://synbiohub.org/public/igem/BBa_K1666004/annotation2445114/range2445114/1
type: Range
persistent identity: range2445114
display id: range2445114
version: 1
direction: direction/forward
top level: BBa_K1666004
start: 1
end: 1593
orientation: inline

BBa_K1313001
type: ComponentDefinition
persistent identity: BBa K1313001
display id: BBa_K1313001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oxoacyl Reductase
creator: Eddie Cano
created: 2014-10-10T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 0
owner id: 19941
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 7-ketocholesterol is the result of the oxidation that exists in the bloodstream. 7-keto group is being reduced by Oxoacyl Reductase into 7-OH. The identity of this enzyme has not yet veryfied. This nucleotidic sequence was based on Jacques Mathieu studies.He identified gene expression analysis using microarrays and in silico amino acidic predictions.
mutable notes: Codon optimization for a better expression on E.coli.
mutable provenance: ATGAAACGCCTGGTCTGTGTTCTGCTGGTCTGCTCGTCTGCTGTTGCTCCGCTGCATCATAGCCTGGTTGATCAC CCGTCTGCCCATCCGGGTCATGGCGGTGGCCCTGTTAGTGCGGGCGTCGCCCTGGTGACCGGTGGCTCCCGTGGC GTTGGTAAAGGCGTCGCAACGGCACTGGGTGCGGCGGGTTGGACCGTGTATGTTACGGGTCGTGGCCCGATGACC GAAGATTGCCCGCTGAGTGCATCCGCTCGTACCGTTACGGGTGCGGGTGGCACCGGTATTGCAGTCGCCTGTGAC CACCGTGAAGATGCAGACGTGCGTGCACTGTTTGCTCGCATCAGTGATGAACAGGCCGGTCGCCTGGACCTGCTG GTTAACAATGTCTGGGCAGCTCCGAAAGGCTTTGGTGGCTTCAGCACCCCGTTCTGGGAACGTCCGCTGGATGAC TGGGATTCTCTGATTGGTGTTGGCCTGCGTGCGCATTATGTGGCCTCAGTTGAAGCAGCACGTCTGATGGTGCCG CGTCGCTCGGGTCTGATTGCGAACATCAGCTCTTTTGGTACCCGCGGCCATCTGCACTCAGTGCTGTACGGCATG TCGAAAGCAGGCCTGGATAAAATGGCTAGCGACATGGCAGCTGAACTGGCCCCGCACGATGTCCGTGCAGTGAGT CTGTGGCCGGGCATTGTGCGCACCGAAATGATCGTTGCGTCCGGTCTGGAAACGATCGCCGGCTTTAGCGTTGCA GATGCTGAATCTCCGGAATTTATTGGTGAAGTCATCTGCGCACTGACCGCCGACCCGACGCTGCCGCAGCGTTCT GGTCATACCTTCGTCACGGCAGAACTGGCTGTGGAATACGGTATTACCGATACGCAAGGTCGTACCCCGCCGAGC CACCGTGAACCGTTTGGTGGCGGTCCGCTGTTCAATATCACG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1313001_sequence/1

BBa_K633000
type: ComponentDefinition
persistent identity: BBa K633000
display id: BBa_K633000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CelD Mutated Cellulase
creator: Camilo Chavez & Thelma Bolon
created: 2011-09-25T11:00:00Z
modified: 2015-06-19T01:53:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cellulase with a mutation, giving it a smalle km
mutable notes: It also has a SacI site and a NheI site after prefix, and before suffix EcorI XbaI SacI Cellulase NheI SpeI PstI
mutable provenance: Source*
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cellulase
sequence: https://synbiohub.org/public/igem/BBa_K633000_sequence/1

Cellulase
type: SequenceAnnotation
persistent identity: annotation2144959
display id: annotation2144959
version: 1
top level: BBa_K633000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K633000/annotation2144959/range2144959/1

https://synbiohub.org/public/igem/BBa_K633000/annotation2144959/range2144959/1
type: Range
persistent identity: range2144959
display id: range2144959
version: 1
direction: direction/forward
top level: BBa_K633000
start: 1
end: 1836
orientation: inline

ninabGen
type: ComponentDefinition
persistent identity: BBa K343005
display id: BBa_K343005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-carotene 15
creator: Christian Kurtzhals
created: 2010-09-06T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
mutable notes: A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
mutable provenance: [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K343001
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343005_sequence/1

BBa_K343001
type: Component
persistent identity: component2079710
display id: component2079710
version: 1
top level: ninabGen
access: public
definition: ninaB

BBa_B0034
type: Component
persistent identity: component2079706
display id: component2079706
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2079700
display id: component2079700
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079706
display id: annotation2079706
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
type: Range
persistent identity: range2079706
display id: range2079706
version: 1
direction: direction/reverse
top level: ninabGen
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2079700
display id: annotation2079700
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
type: Range
persistent identity: range2079700
display id: range2079700
version: 1
direction: direction/forward
top level: ninabGen
start: 1
end: 54
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2079710
display id: annotation2079710
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
type: Range
persistent identity: range2079710
display id: range2079710
version: 1
direction: direction/reverse
top level: ninabGen
start: 81
end: 1943
orientation: inline

BBa_K1554001
type: ComponentDefinition
persistent identity: BBa K1554001
display id: BBa_K1554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AtrΔ11
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Delta11 desaturase
mutable notes: BioBrick compatible
mutable provenance: It comes from Amyelois transitella.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554001_sequence/1

AID
type: ComponentDefinition
persistent identity: BBa K103001
display id: BBa_K103001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AID protein
creator: Michael Lower
created: 2008-09-13T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: AID (Activation Induced Deaminase)is active in mammal lymphocytes, where it causes the somatic hypermutation ??? an increase of mutation level in antibody coding sequences.
mutable provenance: Ref. http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AID
sequence annotation: UGA
sequence annotation: ATG
sequence annotation: NcoI
sequence: https://synbiohub.org/public/igem/BBa_K103001_sequence/1

AID
type: SequenceAnnotation
persistent identity: annotation1975820
display id: annotation1975820
version: 1
top level: AID
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103001/annotation1975820/range1975820/1

https://synbiohub.org/public/igem/BBa_K103001/annotation1975820/range1975820/1
type: Range
persistent identity: range1975820
display id: range1975820
version: 1
direction: direction/forward
top level: AID
start: 3
end: 600
orientation: inline

UGA
type: SequenceAnnotation
persistent identity: annotation1975819
display id: annotation1975819
version: 1
top level: AID
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K103001/annotation1975819/range1975819/1

https://synbiohub.org/public/igem/BBa_K103001/annotation1975819/range1975819/1
type: Range
persistent identity: range1975819
display id: range1975819
version: 1
direction: direction/forward
top level: AID
start: 597
end: 600
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1975818
display id: annotation1975818
version: 1
top level: AID
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K103001/annotation1975818/range1975818/1

https://synbiohub.org/public/igem/BBa_K103001/annotation1975818/range1975818/1
type: Range
persistent identity: range1975818
display id: range1975818
version: 1
direction: direction/forward
top level: AID
start: 3
end: 5
orientation: inline

NcoI
type: SequenceAnnotation
persistent identity: annotation1980044
display id: annotation1980044
version: 1
top level: AID
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K103001/annotation1980044/range1980044/1

https://synbiohub.org/public/igem/BBa_K103001/annotation1980044/range1980044/1
type: Range
persistent identity: range1980044
display id: range1980044
version: 1
direction: direction/forward
top level: AID
start: 1
end: 6
orientation: inline

FadA
type: ComponentDefinition
persistent identity: BBa K861400
display id: BBa_K861400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadA protein coding sequence for fatty acid degradation
creator: Kuanwei Sheng
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1121_
m user id: 0
owner id: 12352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For a E.coli to degrade fatty acid, at least four enzymes ??? FadA, FadB, FadD, FadE and transporter???FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The followed cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB monomers. This part is FadA coding sequence, which has 3-ketoacyl-CoA thiolase activity involving in the β-oxidation of living E.coli. The protein takes a part in the planning system to improve whole efficiency of fatty acid degradation. With appropriate promoters we decided, FadA expression can be under regulation of fatty acid concentration.
mutable notes: Before constructing the final system and testing its function, we constructed three biobrick parts with 3-ketoacyl-CoA thiolase activity (FadA, FadI, S-FadA) as candidates.
mutable provenance: Escherichia coli K.12 MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FadA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K861400_sequence/1

FadA coding sequence
type: SequenceAnnotation
persistent identity: annotation2179595
display id: annotation2179595
version: 1
top level: FadA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K861400/annotation2179595/range2179595/1

https://synbiohub.org/public/igem/BBa_K861400/annotation2179595/range2179595/1
type: Range
persistent identity: range2179595
display id: range2179595
version: 1
direction: direction/forward
top level: FadA
start: 1
end: 1164
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aspartate ammonia-lyase
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: C
sequence annotation: AspA
sequence annotation: Help:Barcodes
sequence annotation: G
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
direction: direction/forward
top level: aspA
start: 1480
end: 1512
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
direction: direction/forward
top level: aspA
start: 588
end: 588
orientation: inline

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1479
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
direction: direction/forward
top level: aspA
start: 1519
end: 1543
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
direction: direction/forward
top level: aspA
start: 999
end: 999
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
direction: direction/forward
top level: aspA
start: 624
end: 624
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
direction: direction/forward
top level: aspA
start: 657
end: 657
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
direction: direction/forward
top level: aspA
start: 1125
end: 1125
orientation: inline

BBa_K1462060
type: ComponentDefinition
persistent identity: BBa K1462060
display id: BBa_K1462060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AdhE2
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462060_sequence/1

katG
type: ComponentDefinition
persistent identity: BBa K137068
display id: BBa_K137068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katG +LAA
creator: Doug Tischer
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is one of two catalases from E. coli. It has a C-term LAA ssrA tag.
mutable notes: Site directed mutagenesis to remove an EcoRI site.
mutable provenance: Genomic PCR off of DH10B
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->T
sequence: https://synbiohub.org/public/igem/BBa_K137068_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation1969499
display id: annotation1969499
version: 1
top level: katG
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1

https://synbiohub.org/public/igem/BBa_K137068/annotation1969499/range1969499/1
type: Range
persistent identity: range1969499
display id: range1969499
version: 1
direction: direction/forward
top level: katG
start: 198
end: 198
orientation: inline

BBa_K389403
type: ComponentDefinition
persistent identity: BBa K389403
display id: BBa_K389403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified BBa_I746390 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part BBa_I746370
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_I746360
component: BBa_I746352
component: BBa_K389004
component: BBa_B0015
sequence annotation: BBa_I746352
sequence annotation: BBa_K389004
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_I746360
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389403_sequence/1

BBa_B0034
type: Component
persistent identity: component2273103
display id: component2273103
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2273117
display id: component2273117
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2273106
display id: component2273106
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I746360
type: Component
persistent identity: component2273115
display id: component2273115
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_I746352
type: Component
persistent identity: component2273101
display id: component2273101
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_K389004
type: Component
persistent identity: component2273120
display id: component2273120
version: 1
top level: BBa_K389403
access: public
definition: luc

BBa_B0015
type: Component
persistent identity: component2273113
display id: component2273113
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2273101
display id: annotation2273101
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
type: Range
persistent identity: range2273101
display id: range2273101
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1
end: 264
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273120
display id: annotation2273120
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
type: Range
persistent identity: range2273120
display id: range2273120
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1259
end: 2914
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273106
display id: annotation2273106
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
type: Range
persistent identity: range2273106
display id: range2273106
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 291
end: 996
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273113
display id: annotation2273113
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273113/range2273113/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389403/annotation2273113/range2273113/1
type: Range
persistent identity: range2273113
display id: range2273113
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1005
end: 1133
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273115
display id: annotation2273115
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
type: Range
persistent identity: range2273115
display id: range2273115
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1142
end: 1232
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273117
display id: annotation2273117
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
type: Range
persistent identity: range2273117
display id: range2273117
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1241
end: 1252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273103
display id: annotation2273103
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
type: Range
persistent identity: range2273103
display id: range2273103
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 273
end: 284
orientation: inline

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

BBa_K808032
type: ComponentDefinition
persistent identity: BBa K808032
display id: BBa_K808032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-03T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
mutable notes: We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.
mutable provenance: assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ara promotor
sequence annotation: BBa_B0029
sequence annotation: AraC
sequence annotation: ara O2
sequence annotation: BBa_K808027
sequence annotation: BBa_K808026
sequence annotation: stop
sequence annotation: ara O1
sequence annotation: 6histag
sequence annotation: myctag
sequence annotation: scar
sequence annotation: BBa_K808028
sequence: https://synbiohub.org/public/igem/BBa_K808032_sequence/1

ara promotor
type: SequenceAnnotation
persistent identity: annotation2183167
display id: annotation2183167
version: 1
top level: BBa_K808032
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183167/range2183167/1
type: Range
persistent identity: range2183167
display id: range2183167
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1153
end: 1181
orientation: inline

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2183169
display id: annotation2183169
version: 1
top level: BBa_K808032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183169/range2183169/1
type: Range
persistent identity: range2183169
display id: range2183169
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1220
end: 1234
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2183164
display id: annotation2183164
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183164/range2183164/1
type: Range
persistent identity: range2183164
display id: range2183164
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1
end: 879
orientation: inline

ara O2
type: SequenceAnnotation
persistent identity: annotation2183165
display id: annotation2183165
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183165/range2183165/1
type: Range
persistent identity: range2183165
display id: range2183165
version: 1
direction: direction/forward
top level: BBa_K808032
start: 909
end: 924
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2183174
display id: annotation2183174
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183174/range2183174/1
type: Range
persistent identity: range2183174
display id: range2183174
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2822
end: 4255
orientation: inline

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2183172
display id: annotation2183172
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183172/range2183172/1
type: Range
persistent identity: range2183172
display id: range2183172
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1322
end: 2791
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183176
display id: annotation2183176
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183176/range2183176/1
type: Range
persistent identity: range2183176
display id: range2183176
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4259
end: 4261
orientation: inline

ara O1
type: SequenceAnnotation
persistent identity: annotation2183166
display id: annotation2183166
version: 1
top level: BBa_K808032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183166/range2183166/1
type: Range
persistent identity: range2183166
display id: range2183166
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1066
end: 1077
orientation: inline

6histag
type: SequenceAnnotation
persistent identity: annotation2183170
display id: annotation2183170
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183170/range2183170/1
type: Range
persistent identity: range2183170
display id: range2183170
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1304
end: 1321
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2183175
display id: annotation2183175
version: 1
top level: BBa_K808032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183175/range2183175/1
type: Range
persistent identity: range2183175
display id: range2183175
version: 1
direction: direction/forward
top level: BBa_K808032
start: 4256
end: 4258
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2183173
display id: annotation2183173
version: 1
top level: BBa_K808032
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183173/range2183173/1
type: Range
persistent identity: range2183173
display id: range2183173
version: 1
direction: direction/forward
top level: BBa_K808032
start: 2792
end: 2821
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2183168
display id: annotation2183168
version: 1
top level: BBa_K808032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183168/range2183168/1
type: Range
persistent identity: range2183168
display id: range2183168
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1211
end: 1218
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2183171
display id: annotation2183171
version: 1
top level: BBa_K808032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1

https://synbiohub.org/public/igem/BBa_K808032/annotation2183171/range2183171/1
type: Range
persistent identity: range2183171
display id: range2183171
version: 1
direction: direction/forward
top level: BBa_K808032
start: 1241
end: 1303
orientation: inline

BBa_K1172205
type: ComponentDefinition
persistent identity: BBa K1172205
display id: BBa_K1172205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli with medium Anderson promoter and medium RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172201
component: BBa_K608006
sequence annotation: BBa_K1172201
sequence annotation: BBa_K608006
sequence: https://synbiohub.org/public/igem/BBa_K1172205_sequence/1

BBa_K1172201
type: Component
persistent identity: component2332949
display id: component2332949
version: 1
top level: BBa_K1172205
access: public
definition: gldA

BBa_K608006
type: Component
persistent identity: component2332947
display id: component2332947
version: 1
top level: BBa_K1172205
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K1172201
type: SequenceAnnotation
persistent identity: annotation2332949
display id: annotation2332949
version: 1
top level: BBa_K1172205
location: https://synbiohub.org/public/igem/BBa_K1172205/annotation2332949/range2332949/1
component: BBa_K1172201

https://synbiohub.org/public/igem/BBa_K1172205/annotation2332949/range2332949/1
type: Range
persistent identity: range2332949
display id: range2332949
version: 1
direction: direction/reverse
top level: BBa_K1172205
start: 63
end: 1166
orientation: inline

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2332947
display id: annotation2332947
version: 1
top level: BBa_K1172205
location: https://synbiohub.org/public/igem/BBa_K1172205/annotation2332947/range2332947/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1172205/annotation2332947/range2332947/1
type: Range
persistent identity: range2332947
display id: range2332947
version: 1
direction: direction/reverse
top level: BBa_K1172205
start: 1
end: 56
orientation: inline

BBa_K656013
type: ComponentDefinition
persistent identity: BBa K656013
display id: BBa_K656013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sporosarcina pasteurii Urease
creator: Andre Burnier, Ryan Kent, Max Song
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _833_
m user id: 0
owner id: 9641
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: Codes for a urease gene, seems to include a promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K1342002
type: ComponentDefinition
persistent identity: BBa K1342002
display id: BBa_K1342002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AspA LVA(-)
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is
mutable notes: soon
mutable provenance: it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1342002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2394009
display id: annotation2394009
version: 1
top level: BBa_K1342002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1

https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1
type: Range
persistent identity: range2394009
display id: range2394009
version: 1
direction: direction/forward
top level: BBa_K1342002
start: 1
end: 1482
orientation: inline

HyaC -> E.
type: ComponentDefinition
persistent identity: BBa K1958003
display id: BBa_K1958003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaC -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:10:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: true
mutable description: HyaC is a subunit of hydrogenase 1 (Hyd-1) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structual subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaC is the third gene of hya operon, sharing the first few base pairs with hyaB and the last few base pairs with hyaD. HyaC is a cytochrome that anchors the [NiFe]-hydrogenase to the membrane.
mutable notes: None.
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: HyaC coding region
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1958003_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493873
display id: annotation2493873
version: 1
top level: HyaC -> E.
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958003/annotation2493873/range2493873/1

https://synbiohub.org/public/igem/BBa_K1958003/annotation2493873/range2493873/1
type: Range
persistent identity: range2493873
display id: range2493873
version: 1
direction: direction/forward
top level: HyaC -> E.
start: 1
end: 3
orientation: inline

HyaC coding region
type: SequenceAnnotation
persistent identity: annotation2493882
display id: annotation2493882
version: 1
top level: HyaC -> E.
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958003/annotation2493882/range2493882/1

https://synbiohub.org/public/igem/BBa_K1958003/annotation2493882/range2493882/1
type: Range
persistent identity: range2493882
display id: range2493882
version: 1
direction: direction/forward
top level: HyaC -> E.
start: 1
end: 708
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2493891
display id: annotation2493891
version: 1
top level: HyaC -> E.
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958003/annotation2493891/range2493891/1

https://synbiohub.org/public/igem/BBa_K1958003/annotation2493891/range2493891/1
type: Range
persistent identity: range2493891
display id: range2493891
version: 1
direction: direction/forward
top level: HyaC -> E.
start: 706
end: 708
orientation: inline

BBa_K993003
type: ComponentDefinition
persistent identity: BBa K993003
display id: BBa_K993003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Urease generator with ROSE thermometer
creator: FURKAN SACİT CEYLAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1290_
m user id: 0
owner id: 11155
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGA…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop Codon
sequence annotation: Start Codon
sequence annotation: ROSE Thermometer
sequence annotation: Urease A
sequence annotation: Insulator
sequence annotation: pVeg2
sequence annotation: RBS
sequence annotation: Urease B
sequence annotation: Urease C
sequence: https://synbiohub.org/public/igem/BBa_K993003_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329471
display id: annotation2329471
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1
type: Range
persistent identity: range2329471
display id: range2329471
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2661
end: 2663
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329468
display id: annotation2329468
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1
type: Range
persistent identity: range2329468
display id: range2329468
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2363
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329458
display id: annotation2329458
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1
type: Range
persistent identity: range2329458
display id: range2329458
version: 1
direction: direction/forward
top level: BBa_K993003
start: 98
end: 193
orientation: inline

Urease A
type: SequenceAnnotation
persistent identity: annotation2329459
display id: annotation2329459
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1
type: Range
persistent identity: range2329459
display id: range2329459
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 1903
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329464
display id: annotation2329464
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1
type: Range
persistent identity: range2329464
display id: range2329464
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2323
end: 2337
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329469
display id: annotation2329469
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1
type: Range
persistent identity: range2329469
display id: range2329469
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1901
end: 1903
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329467
display id: annotation2329467
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1
type: Range
persistent identity: range2329467
display id: range2329467
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 1944
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329447
display id: annotation2329447
version: 1
top level: BBa_K993003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1
type: Range
persistent identity: range2329447
display id: range2329447
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1
end: 97
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329461
display id: annotation2329461
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1
type: Range
persistent identity: range2329461
display id: range2329461
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1919
end: 1941
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329465
display id: annotation2329465
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1
type: Range
persistent identity: range2329465
display id: range2329465
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2338
end: 2360
orientation: inline

Urease B
type: SequenceAnnotation
persistent identity: annotation2329463
display id: annotation2329463
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1
type: Range
persistent identity: range2329463
display id: range2329463
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 2322
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329460
display id: annotation2329460
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1
type: Range
persistent identity: range2329460
display id: range2329460
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1904
end: 1918
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329470
display id: annotation2329470
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1
type: Range
persistent identity: range2329470
display id: range2329470
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2320
end: 2322
orientation: inline

Urease C
type: SequenceAnnotation
persistent identity: annotation2329466
display id: annotation2329466
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1
type: Range
persistent identity: range2329466
display id: range2329466
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2663
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329462
display id: annotation2329462
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1
type: Range
persistent identity: range2329462
display id: range2329462
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 196
orientation: inline

BBa_M36014
type: ComponentDefinition
persistent identity: BBa M36014
display id: BBa_M36014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (alt2)
creator: Jesse Palmer, Elliot Lui
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase) and glgA gene (coding for glycogen synthase).
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
mutable provenance: Composite of genomic sequences coding for proteins involved in e. coli glycogen biosynthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36007
component: BBa_M36010
component: BBa_M36724
component: BBa_M36006
component: BBa_M36009
sequence annotation: BBa_M36010
sequence annotation: BBa_M36724
sequence annotation: BBa_M36007
sequence annotation: BBa_M36009
sequence annotation: BBa_M36006
sequence: https://synbiohub.org/public/igem/BBa_M36014_sequence/1

BBa_M36007
type: Component
persistent identity: component2118547
display id: component2118547
version: 1
top level: BBa_M36014
access: public
definition: BBa_M36007

BBa_M36010
type: Component
persistent identity: component2118548
display id: component2118548
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36724
type: Component
persistent identity: component2118546
display id: component2118546
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36006
type: Component
persistent identity: component2118545
display id: component2118545
version: 1
top level: BBa_M36014
access: public
definition: BBa_M36006

BBa_M36009
type: Component
persistent identity: component2118544
display id: component2118544
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118548
display id: annotation2118548
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118548/range2118548/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36014/annotation2118548/range2118548/1
type: Range
persistent identity: range2118548
display id: range2118548
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 2863
end: 2944
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118546
display id: annotation2118546
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118546/range2118546/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36014/annotation2118546/range2118546/1
type: Range
persistent identity: range2118546
display id: range2118546
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1344
end: 1428
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2118547
display id: annotation2118547
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118547/range2118547/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36014/annotation2118547/range2118547/1
type: Range
persistent identity: range2118547
display id: range2118547
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1429
end: 2862
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118544
display id: annotation2118544
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118544/range2118544/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36014/annotation2118544/range2118544/1
type: Range
persistent identity: range2118544
display id: range2118544
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1
end: 47
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118545
display id: annotation2118545
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118545/range2118545/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36014/annotation2118545/range2118545/1
type: Range
persistent identity: range2118545
display id: range2118545
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 48
end: 1343
orientation: inline

BBa_K808025
type: ComponentDefinition
persistent identity: BBa K808025
display id: BBa_K808025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FsC: Cutinase PET cleaving enzyme
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.
mutable notes: Surface Expression is very hard to do. FsC shows lipolytic activity and attacks the outer membrane of gramm negativ bacteria. This activity leads to a certain toxicity for expression hosts. In addition FsC needs to be expressed in the periplasm due to folding issues.
mutable provenance: Cloned from vector pEST100 from Becker et all 2005: (Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808025_sequence/1

SbpA | Red
type: ComponentDefinition
persistent identity: BBa K525412
display id: BBa_K525412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and NADP+ Oxidoreductase
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PT7 + RBS + SbpA || Red; RCU 25
mutable notes: Assembled
mutable provenance: BBa_K525404; BBa_K525499
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: Reductase
sequence annotation: K525499
sequence annotation: K525401
sequence annotation: ATG
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: SbpA domain
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K525412_sequence/1

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2136968
display id: annotation2136968
version: 1
top level: SbpA | Red
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136968/range2136968/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136968/range2136968/1
type: Range
persistent identity: range2136968
display id: range2136968
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3193
end: 3198
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2136961
display id: annotation2136961
version: 1
top level: SbpA | Red
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136961/range2136961/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136961/range2136961/1
type: Range
persistent identity: range2136961
display id: range2136961
version: 1
direction: direction/forward
top level: SbpA | Red
start: 20
end: 44
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2136970
display id: annotation2136970
version: 1
top level: SbpA | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136970/range2136970/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136970/range2136970/1
type: Range
persistent identity: range2136970
display id: range2136970
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3199
end: 3948
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2136969
display id: annotation2136969
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136969/range2136969/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136969/range2136969/1
type: Range
persistent identity: range2136969
display id: range2136969
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3199
end: 3948
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2136964
display id: annotation2136964
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136964/range2136964/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136964/range2136964/1
type: Range
persistent identity: range2136964
display id: range2136964
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 3193
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2136965
display id: annotation2136965
version: 1
top level: SbpA | Red
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136965/range2136965/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136965/range2136965/1
type: Range
persistent identity: range2136965
display id: range2136965
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 75
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136963
display id: annotation2136963
version: 1
top level: SbpA | Red
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136963/range2136963/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136963/range2136963/1
type: Range
persistent identity: range2136963
display id: range2136963
version: 1
direction: direction/forward
top level: SbpA | Red
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2136962
display id: annotation2136962
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136962/range2136962/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136962/range2136962/1
type: Range
persistent identity: range2136962
display id: range2136962
version: 1
direction: direction/forward
top level: SbpA | Red
start: 53
end: 64
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2136972
display id: annotation2136972
version: 1
top level: SbpA | Red
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136972/range2136972/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136972/range2136972/1
type: Range
persistent identity: range2136972
display id: range2136972
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3946
end: 3948
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2136960
display id: annotation2136960
version: 1
top level: SbpA | Red
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136960/range2136960/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136960/range2136960/1
type: Range
persistent identity: range2136960
display id: range2136960
version: 1
direction: direction/forward
top level: SbpA | Red
start: 1
end: 20
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2136966
display id: annotation2136966
version: 1
top level: SbpA | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136966/range2136966/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136966/range2136966/1
type: Range
persistent identity: range2136966
display id: range2136966
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 3193
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2136971
display id: annotation2136971
version: 1
top level: SbpA | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136971/range2136971/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136971/range2136971/1
type: Range
persistent identity: range2136971
display id: range2136971
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3940
end: 3944
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2136967
display id: annotation2136967
version: 1
top level: SbpA | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136967/range2136967/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136967/range2136967/1
type: Range
persistent identity: range2136967
display id: range2136967
version: 1
direction: direction/forward
top level: SbpA | Red
start: 76
end: 81
orientation: inline

BBa_K361011
type: ComponentDefinition
persistent identity: BBa K361011
display id: BBa_K361011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rci recombinase system
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
mutable notes: This is the optimal system for Rci site-specific recombinase production.
mutable provenance: The promotor is come from lacI regulated one: BBa_R0010 The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034 The terminator is come from a doble terminator with bidirectional termination: BBa_B0014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_S04509
component: BBa_S04524
sequence annotation: BBa_S04524
sequence annotation: BBa_S04509
sequence: https://synbiohub.org/public/igem/BBa_K361011_sequence/1

BBa_S04509
type: Component
persistent identity: component2095262
display id: component2095262
version: 1
top level: BBa_K361011
access: public
definition: https://synbiohub.org/public/igem/BBa_S04509/1

BBa_S04524
type: Component
persistent identity: component2095272
display id: component2095272
version: 1
top level: BBa_K361011
access: public
definition: BBa_S04524

BBa_S04524
type: SequenceAnnotation
persistent identity: annotation2095272
display id: annotation2095272
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
component: BBa_S04524

https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
type: Range
persistent identity: range2095272
display id: range2095272
version: 1
direction: direction/reverse
top level: BBa_K361011
start: 227
end: 1484
orientation: inline

BBa_S04509
type: SequenceAnnotation
persistent identity: annotation2095262
display id: annotation2095262
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
component: BBa_S04509

https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
type: Range
persistent identity: range2095262
display id: range2095262
version: 1
direction: direction/reverse
top level: BBa_K361011
start: 1
end: 220
orientation: inline

LuxI-His
type: ComponentDefinition
persistent identity: BBa K1709000
display id: BBa_K1709000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxI-His
creator: Astrid Deryckere
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T04:12:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Ochre Stop Codon
sequence annotation: ATG Start Codon
sequence annotation: LuxI Coding Region
sequence annotation: Polyhistidine-tag
sequence: https://synbiohub.org/public/igem/BBa_K1709000_sequence/1

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2446992
display id: annotation2446992
version: 1
top level: LuxI-His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446992/range2446992/1

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446992/range2446992/1
type: Range
persistent identity: range2446992
display id: range2446992
version: 1
direction: direction/forward
top level: LuxI-His
start: 607
end: 609
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2446989
display id: annotation2446989
version: 1
top level: LuxI-His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446989/range2446989/1

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446989/range2446989/1
type: Range
persistent identity: range2446989
display id: range2446989
version: 1
direction: direction/forward
top level: LuxI-His
start: 1
end: 3
orientation: inline

LuxI Coding Region
type: SequenceAnnotation
persistent identity: annotation2446990
display id: annotation2446990
version: 1
top level: LuxI-His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446990/range2446990/1

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446990/range2446990/1
type: Range
persistent identity: range2446990
display id: range2446990
version: 1
direction: direction/forward
top level: LuxI-His
start: 1
end: 579
orientation: inline

Polyhistidine-tag
type: SequenceAnnotation
persistent identity: annotation2446991
display id: annotation2446991
version: 1
top level: LuxI-His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709000/annotation2446991/range2446991/1

https://synbiohub.org/public/igem/BBa_K1709000/annotation2446991/range2446991/1
type: Range
persistent identity: range2446991
display id: range2446991
version: 1
direction: direction/forward
top level: LuxI-His
start: 583
end: 606
orientation: inline

metK
type: ComponentDefinition
persistent identity: BBa K1668001
display id: BBa_K1668001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metK (from Streptomyces avermitilis, increasing avermectin production)
creator: iGEM15_ZJU-China
created: 2015-08-13T11:00:00Z
modified: 2015-09-13T01:40:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2086_
m user id: 24510
owner id: 24510
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: coding sequence
mutable notes: overlap
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop coden
sequence annotation: cds
sequence annotation: start coden
sequence: https://synbiohub.org/public/igem/BBa_K1668001_sequence/1

stop coden
type: SequenceAnnotation
persistent identity: annotation2447011
display id: annotation2447011
version: 1
top level: metK
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1
type: Range
persistent identity: range2447011
display id: range2447011
version: 1
direction: direction/forward
top level: metK
start: 811
end: 816
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2456726
display id: annotation2456726
version: 1
top level: metK
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1
type: Range
persistent identity: range2456726
display id: range2456726
version: 1
direction: direction/forward
top level: metK
start: 1
end: 816
orientation: inline

start coden
type: SequenceAnnotation
persistent identity: annotation2447010
display id: annotation2447010
version: 1
top level: metK
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1
type: Range
persistent identity: range2447010
display id: range2447010
version: 1
direction: direction/forward
top level: metK
start: 1
end: 3
orientation: inline

T7Amb
type: ComponentDefinition
persistent identity: BBa K606032
display id: BBa_K606032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA polymerase with tyrosine amber codons
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 gene carrying two amber codons
mutable notes: quick change mutagenesis on T7 (BBa_K145001)
mutable provenance: built on BBa_K145001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7 polymerase
sequence annotation: Amber mutation
sequence annotation: Stop
sequence annotation: stop
sequence annotation: Amber mutation 2
sequence: https://synbiohub.org/public/igem/BBa_K606032_sequence/1

T7 polymerase
type: SequenceAnnotation
persistent identity: annotation2132343
display id: annotation2132343
version: 1
top level: T7Amb
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K606032/annotation2132343/range2132343/1

https://synbiohub.org/public/igem/BBa_K606032/annotation2132343/range2132343/1
type: Range
persistent identity: range2132343
display id: range2132343
version: 1
direction: direction/forward
top level: T7Amb
start: 1
end: 2649
orientation: inline

Amber mutation
type: SequenceAnnotation
persistent identity: annotation2132346
display id: annotation2132346
version: 1
top level: T7Amb
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K606032/annotation2132346/range2132346/1

https://synbiohub.org/public/igem/BBa_K606032/annotation2132346/range2132346/1
type: Range
persistent identity: range2132346
display id: range2132346
version: 1
direction: direction/forward
top level: T7Amb
start: 84
end: 84
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2132344
display id: annotation2132344
version: 1
top level: T7Amb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K606032/annotation2132344/range2132344/1

https://synbiohub.org/public/igem/BBa_K606032/annotation2132344/range2132344/1
type: Range
persistent identity: range2132344
display id: range2132344
version: 1
direction: direction/forward
top level: T7Amb
start: 2650
end: 2652
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2132345
display id: annotation2132345
version: 1
top level: T7Amb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K606032/annotation2132345/range2132345/1

https://synbiohub.org/public/igem/BBa_K606032/annotation2132345/range2132345/1
type: Range
persistent identity: range2132345
display id: range2132345
version: 1
direction: direction/forward
top level: T7Amb
start: 2653
end: 2655
orientation: inline

Amber mutation 2
type: SequenceAnnotation
persistent identity: annotation2132347
display id: annotation2132347
version: 1
top level: T7Amb
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K606032/annotation2132347/range2132347/1

https://synbiohub.org/public/igem/BBa_K606032/annotation2132347/range2132347/1
type: Range
persistent identity: range2132347
display id: range2132347
version: 1
direction: direction/forward
top level: T7Amb
start: 132
end: 132
orientation: inline

BBa_K515000
type: ComponentDefinition
persistent identity: BBa K515000
display id: BBa_K515000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaM - tryptophan-2-mono-oxygenase
creator: Atipat Patharagulpong
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide
mutable notes: No
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Insulator
sequence annotation: rbs
sequence annotation: IaaM
sequence: https://synbiohub.org/public/igem/BBa_K515000_sequence/1

Insulator
type: SequenceAnnotation
persistent identity: annotation2125789
display id: annotation2125789
version: 1
top level: BBa_K515000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K515000/annotation2125789/range2125789/1

https://synbiohub.org/public/igem/BBa_K515000/annotation2125789/range2125789/1
type: Range
persistent identity: range2125789
display id: range2125789
version: 1
direction: direction/forward
top level: BBa_K515000
start: 1
end: 15
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2125790
display id: annotation2125790
version: 1
top level: BBa_K515000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K515000/annotation2125790/range2125790/1

https://synbiohub.org/public/igem/BBa_K515000/annotation2125790/range2125790/1
type: Range
persistent identity: range2125790
display id: range2125790
version: 1
direction: direction/forward
top level: BBa_K515000
start: 16
end: 48
orientation: inline

IaaM
type: SequenceAnnotation
persistent identity: annotation2125791
display id: annotation2125791
version: 1
top level: BBa_K515000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515000/annotation2125791/range2125791/1

https://synbiohub.org/public/igem/BBa_K515000/annotation2125791/range2125791/1
type: Range
persistent identity: range2125791
display id: range2125791
version: 1
direction: direction/forward
top level: BBa_K515000
start: 49
end: 1686
orientation: inline

BBa_K567008
type: ComponentDefinition
persistent identity: BBa K567008
display id: BBa_K567008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-2AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg) (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 2 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28A. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lac operator
sequence annotation: PT7
sequence annotation: rbs
sequence annotation: luciferase
sequence annotation: 2AGG
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K567008_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2150785
display id: annotation2150785
version: 1
top level: BBa_K567008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150785/range2150785/1
type: Range
persistent identity: range2150785
display id: range2150785
version: 1
direction: direction/forward
top level: BBa_K567008
start: 21
end: 44
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2150784
display id: annotation2150784
version: 1
top level: BBa_K567008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150784/range2150784/1
type: Range
persistent identity: range2150784
display id: range2150784
version: 1
direction: direction/forward
top level: BBa_K567008
start: 2
end: 18
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150787
display id: annotation2150787
version: 1
top level: BBa_K567008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150787/range2150787/1
type: Range
persistent identity: range2150787
display id: range2150787
version: 1
direction: direction/forward
top level: BBa_K567008
start: 75
end: 81
orientation: inline

luciferase
type: SequenceAnnotation
persistent identity: annotation2150771
display id: annotation2150771
version: 1
top level: BBa_K567008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150771/range2150771/1
type: Range
persistent identity: range2150771
display id: range2150771
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 1756
orientation: inline

2AGG
type: SequenceAnnotation
persistent identity: annotation2150770
display id: annotation2150770
version: 1
top level: BBa_K567008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150770/range2150770/1
type: Range
persistent identity: range2150770
display id: range2150770
version: 1
direction: direction/forward
top level: BBa_K567008
start: 95
end: 100
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150769
display id: annotation2150769
version: 1
top level: BBa_K567008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1

https://synbiohub.org/public/igem/BBa_K567008/annotation2150769/range2150769/1
type: Range
persistent identity: range2150769
display id: range2150769
version: 1
direction: direction/forward
top level: BBa_K567008
start: 89
end: 91
orientation: inline

BBa_K1587003
type: ComponentDefinition
persistent identity: BBa K1587003
display id: BBa_K1587003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-hydroxybutyryl-CoA dehydratase
creator: M??lissa David
created: 2015-09-12T11:00:00Z
modified: 2015-09-17T04:59:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2004_
m user id: 24598
owner id: 24507
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA.
mutable notes: No information
mutable provenance: C.acetobutylicum sequence found through NCBI: http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2835666&to=2836451&strand=true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crt
sequence: https://synbiohub.org/public/igem/BBa_K1587003_sequence/1

crt
type: SequenceAnnotation
persistent identity: annotation2465508
display id: annotation2465508
version: 1
top level: BBa_K1587003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587003/annotation2465508/range2465508/1

https://synbiohub.org/public/igem/BBa_K1587003/annotation2465508/range2465508/1
type: Range
persistent identity: range2465508
display id: range2465508
version: 1
direction: direction/forward
top level: BBa_K1587003
start: 1
end: 786
orientation: inline

BBa_K1020008
type: ComponentDefinition
persistent identity: BBa K1020008
display id: BBa_K1020008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alkane producing module
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.
mutable notes: AlkR with a weaker promoter and terminator.
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: coding(NPDC)
sequence annotation: strong constitutive promoter
sequence annotation: coding(AAR)
sequence: https://synbiohub.org/public/igem/BBa_K1020008_sequence/1

coding(NPDC)
type: SequenceAnnotation
persistent identity: annotation2353540
display id: annotation2353540
version: 1
top level: BBa_K1020008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1020008/annotation2353540/range2353540/1

https://synbiohub.org/public/igem/BBa_K1020008/annotation2353540/range2353540/1
type: Range
persistent identity: range2353540
display id: range2353540
version: 1
direction: direction/forward
top level: BBa_K1020008
start: 54
end: 753
orientation: inline

strong constitutive promoter
type: SequenceAnnotation
persistent identity: annotation2353331
display id: annotation2353331
version: 1
top level: BBa_K1020008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1020008/annotation2353331/range2353331/1

https://synbiohub.org/public/igem/BBa_K1020008/annotation2353331/range2353331/1
type: Range
persistent identity: range2353331
display id: range2353331
version: 1
direction: direction/forward
top level: BBa_K1020008
start: 1
end: 35
orientation: inline

coding(AAR)
type: SequenceAnnotation
persistent identity: annotation2353541
display id: annotation2353541
version: 1
top level: BBa_K1020008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1020008/annotation2353541/range2353541/1

https://synbiohub.org/public/igem/BBa_K1020008/annotation2353541/range2353541/1
type: Range
persistent identity: range2353541
display id: range2353541
version: 1
direction: direction/forward
top level: BBa_K1020008
start: 777
end: 1803
orientation: inline

BBa_K863015
type: ComponentDefinition
persistent identity: BBa K863015
display id: BBa_K863015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xccl laccase from Xanthomonas campestris with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: xccl laccase from Xanthomonas campestris with pT7 and HIS tag
mutable notes: test
mutable provenance: Xanthomonas campestris pv. campestris B100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: xccl
sequence annotation: TAA
sequence annotation: 6x HIS
sequence annotation: ATG
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K863015_sequence/1

xccl
type: SequenceAnnotation
persistent identity: annotation2198673
display id: annotation2198673
version: 1
top level: BBa_K863015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1
type: Range
persistent identity: range2198673
display id: range2198673
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 1837
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2198697
display id: annotation2198697
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1
type: Range
persistent identity: range2198697
display id: range2198697
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1858
end: 1860
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2198699
display id: annotation2198699
version: 1
top level: BBa_K863015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1
type: Range
persistent identity: range2198699
display id: range2198699
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1838
end: 1857
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2198671
display id: annotation2198671
version: 1
top level: BBa_K863015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1
type: Range
persistent identity: range2198671
display id: range2198671
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 36
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2198670
display id: annotation2198670
version: 1
top level: BBa_K863015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1
type: Range
persistent identity: range2198670
display id: range2198670
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1
end: 23
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2198674
display id: annotation2198674
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1
type: Range
persistent identity: range2198674
display id: range2198674
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1861
end: 1863
orientation: inline

BBa_K1031624
type: ComponentDefinition
persistent identity: BBa K1031624
display id: BBa_K1031624
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031624_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361904
display id: annotation2361904
version: 1
top level: BBa_K1031624
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1
type: Range
persistent identity: range2361904
display id: range2361904
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361905
display id: annotation2361905
version: 1
top level: BBa_K1031624
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1
type: Range
persistent identity: range2361905
display id: range2361905
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1520
end: 1648
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2361903
display id: annotation2361903
version: 1
top level: BBa_K1031624
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1
type: Range
persistent identity: range2361903
display id: range2361903
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1
end: 35
orientation: inline

BBa_K398027
type: ComponentDefinition
persistent identity: BBa K398027
display id: BBa_K398027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LadA Protein Generator (High expression)
creator: Kira Schipper and Mathias Voges
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6322
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LadA, a long-chain alkane monooxygenase from Geobacillus thermodenitrificans NG80-2, facilitates the conversion of long-chain alkanes (up to at least C36) to the corresponding primary alcohols.
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genebank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0040
component: BBa_K398000
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_K398000
sequence: https://synbiohub.org/public/igem/BBa_K398027_sequence/1

BBa_B0034
type: Component
persistent identity: component2083558
display id: component2083558
version: 1
top level: BBa_K398027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2083552
display id: component2083552
version: 1
top level: BBa_K398027
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K398000
type: Component
persistent identity: component2083559
display id: component2083559
version: 1
top level: BBa_K398027
access: public
definition: https://synbiohub.org/public/igem/BBa_K398000/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083558
display id: annotation2083558
version: 1
top level: BBa_K398027
location: https://synbiohub.org/public/igem/BBa_K398027/annotation2083558/range2083558/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K398027/annotation2083558/range2083558/1
type: Range
persistent identity: range2083558
display id: range2083558
version: 1
direction: direction/reverse
top level: BBa_K398027
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083552
display id: annotation2083552
version: 1
top level: BBa_K398027
location: https://synbiohub.org/public/igem/BBa_K398027/annotation2083552/range2083552/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K398027/annotation2083552/range2083552/1
type: Range
persistent identity: range2083552
display id: range2083552
version: 1
direction: direction/forward
top level: BBa_K398027
start: 1
end: 54
orientation: inline

BBa_K398000
type: SequenceAnnotation
persistent identity: annotation2083559
display id: annotation2083559
version: 1
top level: BBa_K398027
location: https://synbiohub.org/public/igem/BBa_K398027/annotation2083559/range2083559/1
component: BBa_K398000

https://synbiohub.org/public/igem/BBa_K398027/annotation2083559/range2083559/1
type: Range
persistent identity: range2083559
display id: range2083559
version: 1
direction: direction/reverse
top level: BBa_K398027
start: 81
end: 1406
orientation: inline

BBa_K1660004
type: ComponentDefinition
persistent identity: BBa K1660004
display id: BBa_K1660004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcyA+Ho1
creator: Dai Yuanyi
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T11:31:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In order to work,Cph8 has to form chromophore with PCB biosynthetic genes, where the formation of PCB requires the gene pcyA and hol to function as accounted below. Hol is a sort of Iron red pigment oxidase which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidase from Synechocystis Sauv, functions to turn biliverdin IXalpha (BV) into PCB. We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduction system in our E.coli.
mutable notes: We used oe-pcr to assemble these parts together seamlessly.
mutable provenance: pcyA gene and ho1 gene both come from Synechocystis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: pcyA barcode
sequence annotation: J23100
sequence annotation: B0034
sequence annotation: start codon
sequence annotation: ho1 barcode
sequence annotation: stop codon
sequence annotation: ho1
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K1660004_sequence/1

pcyA barcode
type: SequenceAnnotation
persistent identity: annotation2445869
display id: annotation2445869
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1
type: Range
persistent identity: range2445869
display id: range2445869
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 798
end: 822
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2445845
display id: annotation2445845
version: 1
top level: BBa_K1660004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1
type: Range
persistent identity: range2445845
display id: range2445845
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445884
display id: annotation2445884
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1
type: Range
persistent identity: range2445884
display id: range2445884
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 823
end: 834
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445938
display id: annotation2445938
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1
type: Range
persistent identity: range2445938
display id: range2445938
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 837
orientation: inline

ho1 barcode
type: SequenceAnnotation
persistent identity: annotation2445942
display id: annotation2445942
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1
type: Range
persistent identity: range2445942
display id: range2445942
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1561
end: 1585
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445943
display id: annotation2445943
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1
type: Range
persistent identity: range2445943
display id: range2445943
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1558
end: 1560
orientation: inline

ho1
type: SequenceAnnotation
persistent identity: annotation2445885
display id: annotation2445885
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1
type: Range
persistent identity: range2445885
display id: range2445885
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 1560
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2445867
display id: annotation2445867
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1
type: Range
persistent identity: range2445867
display id: range2445867
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 750
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445865
display id: annotation2445865
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1
type: Range
persistent identity: range2445865
display id: range2445865
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 50
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445868
display id: annotation2445868
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1
type: Range
persistent identity: range2445868
display id: range2445868
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 792
end: 797
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445864
display id: annotation2445864
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1
type: Range
persistent identity: range2445864
display id: range2445864
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 36
end: 47
orientation: inline

BBa_K156011
type: ComponentDefinition
persistent identity: BBa K156011
display id: BBa_K156011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadA (streptomycin 3'-adenyltransferase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156011_sequence/1

BBa_K2127001
type: ComponentDefinition
persistent identity: BBa K2127001
display id: BBa_K2127001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible High Expression His-Tagged Photosystem II CP47 subunit
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:45:18Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 0
m user id: 32415
owner id: 32415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TFBS plac LacO CP47his
mutable notes: synthesized
mutable provenance: Synchocisytys
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K2127004
component: BBa_R0010
component: BBa_K2127007
component: BBa_K2127006
component: BBa_B0030
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127004
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127006
sequence: https://synbiohub.org/public/igem/BBa_K2127001_sequence/1

BBa_K2127004
type: Component
persistent identity: component2527081
display id: component2527081
version: 1
top level: BBa_K2127001
access: public
definition: CP47His

BBa_R0010
type: Component
persistent identity: component2527067
display id: component2527067
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127007
type: Component
persistent identity: component2527082
display id: component2527082
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_K2127006
type: Component
persistent identity: component2527066
display id: component2527066
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127006/1

BBa_B0030
type: Component
persistent identity: component2527075
display id: component2527075
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527082
display id: annotation2527082
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
type: Range
persistent identity: range2527082
display id: range2527082
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1949
end: 2071
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527081
display id: annotation2527081
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
type: Range
persistent identity: range2527081
display id: range2527081
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 401
end: 1948
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527075
display id: annotation2527075
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
type: Range
persistent identity: range2527075
display id: range2527075
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 386
end: 400
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527067
display id: annotation2527067
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
type: Range
persistent identity: range2527067
display id: range2527067
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 186
end: 385
orientation: inline

BBa_K2127006
type: SequenceAnnotation
persistent identity: annotation2527066
display id: annotation2527066
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
component: BBa_K2127006

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
type: Range
persistent identity: range2527066
display id: range2527066
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1
end: 185
orientation: inline

dddD
type: ComponentDefinition
persistent identity: BBa K1312002
display id: BBa_K1312002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dddD gene from Ruegeria pomeroyi.
creator: Yutaka Murata
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1687_
m user id: 0
owner id: 17633
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is dddD gene from the genome of Ruegeria pomperoyi. This gene code DddD protein which catalyze breakdown of DMSP and create DMS.
mutable notes: There was PstⅠ recognition site so I made single base substitution in recognition site.
mutable provenance: The genome of Ruegeria pomperoyi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dddD
sequence: https://synbiohub.org/public/igem/BBa_K1312002_sequence/1

dddD
type: SequenceAnnotation
persistent identity: annotation2428830
display id: annotation2428830
version: 1
top level: dddD
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1312002/annotation2428830/range2428830/1

https://synbiohub.org/public/igem/BBa_K1312002/annotation2428830/range2428830/1
type: Range
persistent identity: range2428830
display id: range2428830
version: 1
direction: direction/forward
top level: dddD
start: 1
end: 2481
orientation: inline

BBa_K112233
type: ComponentDefinition
persistent identity: BBa K112233
display id: BBa_K112233
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < phoA >
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The phoA enzyme without start or stop codons. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of {<phoA>} Biobrick Part K112233 PCR mea036/mea037 on pBca9145-Bca1126 (1219 bp, EcoRI/BamHI) Sub into pBjh1601AC-Bca1144 (EcoRI/BamHI, 3195,910 L) Product is pBjh1601AC-K112233 ---------------------------------------- mea036 Forward Biobricking of {<phoA>} cgatagaattcatgAGATCTGGAGATTCTG mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112233_sequence/1

HyaE -> E
type: ComponentDefinition
persistent identity: BBa K1958000
display id: BBa_K1958000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaE -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:20:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: true
mutable description: HyaE is a subunit of hydrogenase 1 (Hyd-1) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structual subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaE is the fifth gene of hya operon, sharing the first few base pairs with hyaD and the last few base pairs with hyaF. HyaE is a hydrogenase maturase. It is important to note that hyaE is not required for the assembly of standard O2-sensitive hydrogenases (Hyd-2 and others) and are apparently only involved in the assembly of O2-tolerant respiratory enzymes.
mutable notes: None.
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence annotation: HyaE coding region
sequence: https://synbiohub.org/public/igem/BBa_K1958000_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493922
display id: annotation2493922
version: 1
top level: HyaE -> E
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958000/annotation2493922/range2493922/1

https://synbiohub.org/public/igem/BBa_K1958000/annotation2493922/range2493922/1
type: Range
persistent identity: range2493922
display id: range2493922
version: 1
direction: direction/forward
top level: HyaE -> E
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2493925
display id: annotation2493925
version: 1
top level: HyaE -> E
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958000/annotation2493925/range2493925/1

https://synbiohub.org/public/igem/BBa_K1958000/annotation2493925/range2493925/1
type: Range
persistent identity: range2493925
display id: range2493925
version: 1
direction: direction/forward
top level: HyaE -> E
start: 397
end: 399
orientation: inline

HyaE coding region
type: SequenceAnnotation
persistent identity: annotation2493923
display id: annotation2493923
version: 1
top level: HyaE -> E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958000/annotation2493923/range2493923/1

https://synbiohub.org/public/igem/BBa_K1958000/annotation2493923/range2493923/1
type: Range
persistent identity: range2493923
display id: range2493923
version: 1
direction: direction/forward
top level: HyaE -> E
start: 1
end: 399
orientation: inline

TdcE
type: ComponentDefinition
persistent identity: BBa K348000
display id: BBa_K348000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TdCE - pyruvate formate lyase
creator: Laurence Van Melderen
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _473_
m user id: 0
owner id: 4079
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 2-ketobutyrate formate-lyase/pyruvate formate-lyase 4. It catalyzes the reversible conversion of pyruvate and coenzyme-A into formate and acetyl-CoA; the formate can then be used for Hydrogen production. It is activated under anaerobic conditions, we wanted to constitutively express it in order to improve the formate production.
mutable notes: It was amplified by PCR using those primers : CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT
mutable provenance: It comes from the genome of Escherichia coli K-12 substr. MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TdcE
sequence: https://synbiohub.org/public/igem/BBa_K348000_sequence/1

TdcE
type: SequenceAnnotation
persistent identity: annotation2108783
display id: annotation2108783
version: 1
top level: TdcE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K348000/annotation2108783/range2108783/1

https://synbiohub.org/public/igem/BBa_K348000/annotation2108783/range2108783/1
type: Range
persistent identity: range2108783
display id: range2108783
version: 1
direction: direction/forward
top level: TdcE
start: 1
end: 2295
orientation: inline

KanR
type: ComponentDefinition
persistent identity: BBa J31003
display id: BBa_J31003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kanamycin resistance forward (KanF) [cf. BBa_J23012 & BBa_J31002]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: If there is a promoter and an RBS before this region, the cell will express Kanamycin resistance. As it has full biobricks, you can easily add KanR to an existing part without changing the plasmid vector,
mutable notes: This part is cloned in plasmid pSB1A2.
mutable provenance: pSB1AK3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KanR Gene
sequence: https://synbiohub.org/public/igem/BBa_J31003_sequence/1

KanR Gene
type: SequenceAnnotation
persistent identity: annotation1884996
display id: annotation1884996
version: 1
top level: KanR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1

https://synbiohub.org/public/igem/BBa_J31003/annotation1884996/range1884996/1
type: Range
persistent identity: range1884996
display id: range1884996
version: 1
direction: direction/forward
top level: KanR
start: 1
end: 816
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa I732005
display id: BBa_I732005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ (encoding beta-galactosidase, full-length)
creator: Zhan Jian
created: 2007-07-05T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacZ encodes beta-galactosidase, an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. Beta-galactosidase can also cleaves X-gal and ONPG into colorful products.
mutable notes: None
mutable provenance: PCR amplified and standardized from BL21
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacZ
sequence annotation: C->T
sequence annotation: START
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732005_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation1936870
display id: annotation1936870
version: 1
top level: LacZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
type: Range
persistent identity: range1936870
display id: range1936870
version: 1
direction: direction/forward
top level: LacZ
start: 1
end: 3075
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1936869
display id: annotation1936869
version: 1
top level: LacZ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
type: Range
persistent identity: range1936869
display id: range1936869
version: 1
direction: direction/forward
top level: LacZ
start: 3024
end: 3024
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1936871
display id: annotation1936871
version: 1
top level: LacZ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
type: Range
persistent identity: range1936871
display id: range1936871
version: 1
direction: direction/forward
top level: LacZ
start: 1
end: 3
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936872
display id: annotation1936872
version: 1
top level: LacZ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
type: Range
persistent identity: range1936872
display id: range1936872
version: 1
direction: direction/forward
top level: LacZ
start: 3073
end: 3075
orientation: inline

BBa_K1159000
type: ComponentDefinition
persistent identity: BBa K1159000
display id: BBa_K1159000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erythromycin Esterase Type II (EreB) in RFC[25]
creator: Louise FUNKE
created: 2013-05-16T11:00:00Z
modified: 2015-05-28T02:59:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Erythromycin Esterase Type II
sequence: https://synbiohub.org/public/igem/BBa_K1159000_sequence/1

Erythromycin Esterase Type II
type: SequenceAnnotation
persistent identity: annotation2342950
display id: annotation2342950
version: 1
top level: BBa_K1159000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159000/annotation2342950/range2342950/1

https://synbiohub.org/public/igem/BBa_K1159000/annotation2342950/range2342950/1
type: Range
persistent identity: range2342950
display id: range2342950
version: 1
direction: direction/forward
top level: BBa_K1159000
start: 1
end: 1254
orientation: inline

ninaB
type: ComponentDefinition
persistent identity: BBa K343001
display id: BBa_K343001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.
creator: Christian Kurtzhals
created: 2010-07-01T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This coding region encodes a beta-carotene deoxygenase, that splits beta-carotene into two retinal molecules.
mutable notes: No considerations have been made so far concerning design
mutable provenance: Sequence taken from cDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: stop
sequence annotation: met
sequence: https://synbiohub.org/public/igem/BBa_K343001_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2072319
display id: annotation2072319
version: 1
top level: ninaB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1
type: Range
persistent identity: range2072319
display id: range2072319
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 1860
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2072321
display id: annotation2072321
version: 1
top level: ninaB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1
type: Range
persistent identity: range2072321
display id: range2072321
version: 1
direction: direction/forward
top level: ninaB
start: 1861
end: 1863
orientation: inline

met
type: SequenceAnnotation
persistent identity: annotation2072320
display id: annotation2072320
version: 1
top level: ninaB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1
type: Range
persistent identity: range2072320
display id: range2072320
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 3
orientation: inline

BBa_K567006
type: ComponentDefinition
persistent identity: BBa K567006
display id: BBa_K567006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbla-Luc-6AGG
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: β-lactamase promoter-Luciferase with six AGG-codon insertions. This biobrick is constructed by putting modified enzyme luciferase under constituitive promoter β-lactamase promoter. 6 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg)(BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin and 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: Point mutation is used to obtain this part from BBa_K567005
mutable provenance: β-lactamase promoter is derived from pUC18 β-lactamase operon. β-lactamase promoter-Luciferase with four AGG-codon insertions is from BBa_K567005, then point mutated to six AGG-codon insertions
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: ATG
sequence annotation: 6AGG
sequence annotation: luciferase-6AGG
sequence annotation: Pbla
sequence: https://synbiohub.org/public/igem/BBa_K567006_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2154573
display id: annotation2154573
version: 1
top level: BBa_K567006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567006/annotation2154573/range2154573/1

https://synbiohub.org/public/igem/BBa_K567006/annotation2154573/range2154573/1
type: Range
persistent identity: range2154573
display id: range2154573
version: 1
direction: direction/forward
top level: BBa_K567006
start: 278
end: 282
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150761
display id: annotation2150761
version: 1
top level: BBa_K567006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567006/annotation2150761/range2150761/1

https://synbiohub.org/public/igem/BBa_K567006/annotation2150761/range2150761/1
type: Range
persistent identity: range2150761
display id: range2150761
version: 1
direction: direction/forward
top level: BBa_K567006
start: 287
end: 289
orientation: inline

6AGG
type: SequenceAnnotation
persistent identity: annotation2150762
display id: annotation2150762
version: 1
top level: BBa_K567006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567006/annotation2150762/range2150762/1

https://synbiohub.org/public/igem/BBa_K567006/annotation2150762/range2150762/1
type: Range
persistent identity: range2150762
display id: range2150762
version: 1
direction: direction/forward
top level: BBa_K567006
start: 290
end: 307
orientation: inline

luciferase-6AGG
type: SequenceAnnotation
persistent identity: annotation2150763
display id: annotation2150763
version: 1
top level: BBa_K567006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567006/annotation2150763/range2150763/1

https://synbiohub.org/public/igem/BBa_K567006/annotation2150763/range2150763/1
type: Range
persistent identity: range2150763
display id: range2150763
version: 1
direction: direction/forward
top level: BBa_K567006
start: 287
end: 1963
orientation: inline

Pbla
type: SequenceAnnotation
persistent identity: annotation2152728
display id: annotation2152728
version: 1
top level: BBa_K567006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567006/annotation2152728/range2152728/1

https://synbiohub.org/public/igem/BBa_K567006/annotation2152728/range2152728/1
type: Range
persistent identity: range2152728
display id: range2152728
version: 1
direction: direction/forward
top level: BBa_K567006
start: 217
end: 244
orientation: inline

BBa_K1554003
type: ComponentDefinition
persistent identity: BBa K1554003
display id: BBa_K1554003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EaDAcT
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:29:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: diacylglycerol acetyltransferase
mutable notes: BioBrick compatible
mutable provenance: It comes from Euonymus alatus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554003_sequence/1

BBa_K863021
type: ComponentDefinition
persistent identity: BBa K863021
display id: BBa_K863021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans
creator: Isabel Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CDS
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863021_sequence/1

CDS
type: SequenceAnnotation
persistent identity: annotation2213229
display id: annotation2213229
version: 1
top level: BBa_K863021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1
type: Range
persistent identity: range2213229
display id: range2213229
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 1503
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213230
display id: annotation2213230
version: 1
top level: BBa_K863021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1
type: Range
persistent identity: range2213230
display id: range2213230
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2213231
display id: annotation2213231
version: 1
top level: BBa_K863021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1
type: Range
persistent identity: range2213231
display id: range2213231
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1501
end: 1503
orientation: inline

BBa_K1037000
type: ComponentDefinition
persistent identity: BBa K1037000
display id: BBa_K1037000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ygfG
creator: Zeng Jianhao
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1344_
m user id: 0
owner id: 11853
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: methylmalonyl-CoA decarboxylase;catalyzes the decarboxylation of methylmalonyl-CoA to propionyl-CoA.
mutable notes: mutations to remove restriction sites
mutable provenance: E.Coli K12. Sequence found through GeneBank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ygfG coding region
sequence annotation: TAA stop coden
sequence annotation: ATG start coden
sequence: https://synbiohub.org/public/igem/BBa_K1037000_sequence/1

ygfG coding region
type: SequenceAnnotation
persistent identity: annotation2337493
display id: annotation2337493
version: 1
top level: BBa_K1037000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1037000/annotation2337493/range2337493/1

https://synbiohub.org/public/igem/BBa_K1037000/annotation2337493/range2337493/1
type: Range
persistent identity: range2337493
display id: range2337493
version: 1
direction: direction/forward
top level: BBa_K1037000
start: 1
end: 786
orientation: inline

TAA stop coden
type: SequenceAnnotation
persistent identity: annotation2337494
display id: annotation2337494
version: 1
top level: BBa_K1037000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1037000/annotation2337494/range2337494/1

https://synbiohub.org/public/igem/BBa_K1037000/annotation2337494/range2337494/1
type: Range
persistent identity: range2337494
display id: range2337494
version: 1
direction: direction/forward
top level: BBa_K1037000
start: 784
end: 786
orientation: inline

ATG start coden
type: SequenceAnnotation
persistent identity: annotation2337492
display id: annotation2337492
version: 1
top level: BBa_K1037000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1037000/annotation2337492/range2337492/1

https://synbiohub.org/public/igem/BBa_K1037000/annotation2337492/range2337492/1
type: Range
persistent identity: range2337492
display id: range2337492
version: 1
direction: direction/forward
top level: BBa_K1037000
start: 1
end: 3
orientation: inline

BBa_K1122672
type: ComponentDefinition
persistent identity: BBa K1122672
display id: BBa_K1122672
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alcohol dehydrogenase E (AdhE)
creator: Aleksandra Lewicka, Jan Lyczakowski
created: 2013-08-08T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part codes for AdhE protein from ''Clostridium acetobutylicum''. The enzyme is a bi-functional molecule and enables conversion of Acetyl coenzyme A to ethanol.
mutable notes: mutagenesis of two SpeI sites.
mutable provenance: gDNA of Weizmann Organism: ''Clostridium acetobutylicum''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122672_sequence/1

BBa_M36705
type: ComponentDefinition
persistent identity: BBa M36705
display id: BBa_M36705
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Benzalacetone Reductase
creator: Paul Chen, Sean Valle, Svetlana Lyalina
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9644
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Coding sequence for the enzyme benzalacetone reductase which reduces 4-hydroxybenzalacetone to raspberry ketone.
mutable notes: For educational purposes only.
mutable provenance: Beekwilder, Martinus J., Ole Sibbesen, Joern D. Mikkelsen, Ingrid M. Van Der Meer, Robert D. Hall, and Ingmer Qvist. Synthesis of Benzalacetone/raspberry Ketone by Chalcone Synthase. Danisco, assignee. Patent GB2416770A. 8 Feb. 2006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_M36705_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2119135
display id: annotation2119135
version: 1
top level: BBa_M36705
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36705/annotation2119135/range2119135/1

https://synbiohub.org/public/igem/BBa_M36705/annotation2119135/range2119135/1
type: Range
persistent identity: range2119135
display id: range2119135
version: 1
direction: direction/forward
top level: BBa_M36705
start: 928
end: 930
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2119134
display id: annotation2119134
version: 1
top level: BBa_M36705
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36705/annotation2119134/range2119134/1

https://synbiohub.org/public/igem/BBa_M36705/annotation2119134/range2119134/1
type: Range
persistent identity: range2119134
display id: range2119134
version: 1
direction: direction/forward
top level: BBa_M36705
start: 1
end: 3
orientation: inline

BBa_K2127002
type: ComponentDefinition
persistent identity: BBa K2127002
display id: BBa_K2127002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP47 His-tagged with Lac Promoter
creator: Dawson Zeng
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:46:10Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 17480
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
mutable notes: Double Stop Codon. ORF Check
mutable provenance: NCBI Reference Sequence: NC_000911.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K2127004
component: BBa_R0010
component: BBa_K2127007
component: BBa_B0030
sequence annotation: BBa_K2127004
sequence annotation: BBa_K2127007
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K2127002_sequence/1

BBa_K2127004
type: Component
persistent identity: component2527113
display id: component2527113
version: 1
top level: BBa_K2127002
access: public
definition: CP47His

BBa_R0010
type: Component
persistent identity: component2527099
display id: component2527099
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127007
type: Component
persistent identity: component2527114
display id: component2527114
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_B0030
type: Component
persistent identity: component2527107
display id: component2527107
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527113
display id: annotation2527113
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
type: Range
persistent identity: range2527113
display id: range2527113
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 216
end: 1763
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527114
display id: annotation2527114
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
type: Range
persistent identity: range2527114
display id: range2527114
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 1764
end: 1886
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527099
display id: annotation2527099
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
type: Range
persistent identity: range2527099
display id: range2527099
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527107
display id: annotation2527107
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
type: Range
persistent identity: range2527107
display id: range2527107
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 201
end: 215
orientation: inline

BBa_K1037003
type: ComponentDefinition
persistent identity: BBa K1037003
display id: BBa_K1037003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ygfH
creator: Zeng Jianhao
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1344_
m user id: 0
owner id: 11853
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: succinate CoA transferase
mutable notes: base mutation
mutable provenance: E.coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA stop coden
sequence annotation: ATG start coden
sequence annotation: ygfH coding region
sequence: https://synbiohub.org/public/igem/BBa_K1037003_sequence/1

TAA stop coden
type: SequenceAnnotation
persistent identity: annotation2337627
display id: annotation2337627
version: 1
top level: BBa_K1037003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1037003/annotation2337627/range2337627/1

https://synbiohub.org/public/igem/BBa_K1037003/annotation2337627/range2337627/1
type: Range
persistent identity: range2337627
display id: range2337627
version: 1
direction: direction/forward
top level: BBa_K1037003
start: 1477
end: 1479
orientation: inline

ATG start coden
type: SequenceAnnotation
persistent identity: annotation2337624
display id: annotation2337624
version: 1
top level: BBa_K1037003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1037003/annotation2337624/range2337624/1

https://synbiohub.org/public/igem/BBa_K1037003/annotation2337624/range2337624/1
type: Range
persistent identity: range2337624
display id: range2337624
version: 1
direction: direction/forward
top level: BBa_K1037003
start: 1
end: 3
orientation: inline

ygfH coding region
type: SequenceAnnotation
persistent identity: annotation2337626
display id: annotation2337626
version: 1
top level: BBa_K1037003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1037003/annotation2337626/range2337626/1

https://synbiohub.org/public/igem/BBa_K1037003/annotation2337626/range2337626/1
type: Range
persistent identity: range2337626
display id: range2337626
version: 1
direction: direction/forward
top level: BBa_K1037003
start: 1
end: 1479
orientation: inline

BBa_K777122
type: ComponentDefinition
persistent identity: BBa K777122
display id: BBa_K777122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K777121
component: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K777121
sequence: https://synbiohub.org/public/igem/BBa_K777122_sequence/1

BBa_B0034
type: Component
persistent identity: component2191155
display id: component2191155
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K777121
type: Component
persistent identity: component2191157
display id: component2191157
version: 1
top level: BBa_K777122
access: public
definition: BBa_K777121

BBa_J23100
type: Component
persistent identity: component2191153
display id: component2191153
version: 1
top level: BBa_K777122
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2191155
display id: annotation2191155
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K777122/annotation2191155/range2191155/1
type: Range
persistent identity: range2191155
display id: range2191155
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2191153
display id: annotation2191153
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777122/annotation2191153/range2191153/1
type: Range
persistent identity: range2191153
display id: range2191153
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 1
end: 35
orientation: inline

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2191157
display id: annotation2191157
version: 1
top level: BBa_K777122
location: https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777122/annotation2191157/range2191157/1
type: Range
persistent identity: range2191157
display id: range2191157
version: 1
direction: direction/reverse
top level: BBa_K777122
start: 62
end: 829
orientation: inline

T25
type: ComponentDefinition
persistent identity: BBa K1218016
display id: BBa_K1218016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bordetella pertussis adenylate cyclase subunit T25
creator: Alex Constantino
created: 2013-08-28T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1532_
m user id: 0
owner id: 17291
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: One component of a two-hybrid assay. Synthesizes cAMP when it comes in proximity to T18.
mutable notes: Based on the standard Bacterial Adenylate Cyclase Two-Hybrid assay.
mutable provenance: PCR amplified from Bordetella pertussis crude cell lysate
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cds
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1218016_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2333509
display id: annotation2333509
version: 1
top level: T25
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1218016/annotation2333509/range2333509/1

https://synbiohub.org/public/igem/BBa_K1218016/annotation2333509/range2333509/1
type: Range
persistent identity: range2333509
display id: range2333509
version: 1
direction: direction/forward
top level: T25
start: 673
end: 675
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2333510
display id: annotation2333510
version: 1
top level: T25
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1218016/annotation2333510/range2333510/1

https://synbiohub.org/public/igem/BBa_K1218016/annotation2333510/range2333510/1
type: Range
persistent identity: range2333510
display id: range2333510
version: 1
direction: direction/forward
top level: T25
start: 1
end: 675
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2333508
display id: annotation2333508
version: 1
top level: T25
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1218016/annotation2333508/range2333508/1

https://synbiohub.org/public/igem/BBa_K1218016/annotation2333508/range2333508/1
type: Range
persistent identity: range2333508
display id: range2333508
version: 1
direction: direction/forward
top level: T25
start: 1
end: 3
orientation: inline

BBa_K808030
type: ComponentDefinition
persistent identity: BBa K808030
display id: BBa_K808030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
mutable notes: We got it synthesized by GENEART but added the RBS via PCR.
mutable provenance: This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0029
sequence annotation: BBa_K808028
sequence annotation: BBa_K808026
sequence annotation: BBa_K808027
sequence annotation: start
sequence annotation: stop
sequence annotation: myctag
sequence annotation: 6 histag
sequence: https://synbiohub.org/public/igem/BBa_K808030_sequence/1

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2182926
display id: annotation2182926
version: 1
top level: BBa_K808030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1
type: Range
persistent identity: range2182926
display id: range2182926
version: 1
direction: direction/forward
top level: BBa_K808030
start: 2
end: 16
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2182934
display id: annotation2182934
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1
type: Range
persistent identity: range2182934
display id: range2182934
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 85
orientation: inline

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2182935
display id: annotation2182935
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1
type: Range
persistent identity: range2182935
display id: range2182935
version: 1
direction: direction/forward
top level: BBa_K808030
start: 104
end: 1573
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2182937
display id: annotation2182937
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1
type: Range
persistent identity: range2182937
display id: range2182937
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1604
end: 3037
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2182940
display id: annotation2182940
version: 1
top level: BBa_K808030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1
type: Range
persistent identity: range2182940
display id: range2182940
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 25
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182938
display id: annotation2182938
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1
type: Range
persistent identity: range2182938
display id: range2182938
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3038
end: 3040
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2182936
display id: annotation2182936
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1
type: Range
persistent identity: range2182936
display id: range2182936
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1574
end: 1603
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182939
display id: annotation2182939
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1
type: Range
persistent identity: range2182939
display id: range2182939
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3041
end: 3043
orientation: inline

6 histag
type: SequenceAnnotation
persistent identity: annotation2182933
display id: annotation2182933
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1
type: Range
persistent identity: range2182933
display id: range2182933
version: 1
direction: direction/forward
top level: BBa_K808030
start: 86
end: 103
orientation: inline

Rci
type: ComponentDefinition
persistent identity: BBa K361000
display id: BBa_K361000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rci site-specific recombinase
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition. After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
mutable notes: The gene is come from muliple inversion systems in R64 plasmid and there is no further modification. Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
mutable provenance: It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rci recombinase
sequence: https://synbiohub.org/public/igem/BBa_K361000_sequence/1

Rci recombinase
type: SequenceAnnotation
persistent identity: annotation2095181
display id: annotation2095181
version: 1
top level: Rci
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1

https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1
type: Range
persistent identity: range2095181
display id: range2095181
version: 1
direction: direction/forward
top level: Rci
start: 1
end: 1155
orientation: inline

BBa_K2023003
type: ComponentDefinition
persistent identity: BBa K2023003
display id: BBa_K2023003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gluc coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:23:39Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gluc coding device with Pu
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I723020
component: BBa_B0014
component: BBa_K2023009
component: BBa_B0034
sequence annotation: BBa_B0014
sequence annotation: BBa_K2023009
sequence annotation: BBa_I723020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K2023003_sequence/1

BBa_I723020
type: Component
persistent identity: component2518684
display id: component2518684
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0014
type: Component
persistent identity: component2518695
display id: component2518695
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K2023009
type: Component
persistent identity: component2518688
display id: component2518688
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023009/1

BBa_B0034
type: Component
persistent identity: component2518686
display id: component2518686
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518695
display id: annotation2518695
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
type: Range
persistent identity: range2518695
display id: range2518695
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 916
end: 1010
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2518688
display id: annotation2518688
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
type: Range
persistent identity: range2518688
display id: range2518688
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 347
end: 907
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518684
display id: annotation2518684
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
type: Range
persistent identity: range2518684
display id: range2518684
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 1
end: 320
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518686
display id: annotation2518686
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
type: Range
persistent identity: range2518686
display id: range2518686
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 329
end: 340
orientation: inline

BBa_K646003
type: ComponentDefinition
persistent identity: BBa K646003
display id: BBa_K646003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter with Hemeoxygenase
creator: David Dubow
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _822_
m user id: 0
owner id: 10455
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin.
mutable notes: None.
mutable provenance: BBa_K646000 and BBa_I719005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K646000
sequence annotation: BBa_K646000
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K646003_sequence/1

BBa_I719005
type: Component
persistent identity: component2152628
display id: component2152628
version: 1
top level: BBa_K646003
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K646000
type: Component
persistent identity: component2152631
display id: component2152631
version: 1
top level: BBa_K646003
access: public
definition: HO1

BBa_K646000
type: SequenceAnnotation
persistent identity: annotation2152631
display id: annotation2152631
version: 1
top level: BBa_K646003
location: https://synbiohub.org/public/igem/BBa_K646003/annotation2152631/range2152631/1
component: BBa_K646000

https://synbiohub.org/public/igem/BBa_K646003/annotation2152631/range2152631/1
type: Range
persistent identity: range2152631
display id: range2152631
version: 1
direction: direction/reverse
top level: BBa_K646003
start: 32
end: 775
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2152628
display id: annotation2152628
version: 1
top level: BBa_K646003
location: https://synbiohub.org/public/igem/BBa_K646003/annotation2152628/range2152628/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K646003/annotation2152628/range2152628/1
type: Range
persistent identity: range2152628
display id: range2152628
version: 1
direction: direction/reverse
top level: BBa_K646003
start: 1
end: 23
orientation: inline

BBa_K1031623
type: ComponentDefinition
persistent identity: BBa K1031623
display id: BBa_K1031623
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23113
sequence annotation: NahF
sequence: https://synbiohub.org/public/igem/BBa_K1031623_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361894
display id: annotation2361894
version: 1
top level: BBa_K1031623
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1
type: Range
persistent identity: range2361894
display id: range2361894
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1520
end: 1648
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361890
display id: annotation2361890
version: 1
top level: BBa_K1031623
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1
type: Range
persistent identity: range2361890
display id: range2361890
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361891
display id: annotation2361891
version: 1
top level: BBa_K1031623
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1
type: Range
persistent identity: range2361891
display id: range2361891
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 62
end: 1513
orientation: inline

BBa_K1122676
type: ComponentDefinition
persistent identity: BBa K1122676
display id: BBa_K1122676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible pdc and adhB
creator: Aleksandra Lewicka, Jan Lyczakowski
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This parts enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) using IPTG induction. Those enzymes are involved in conversion of pyruvate to ethanol via an acetaldehyde intermediate. The pdc and adhB sequences come from ''Zymomonas mobilis'' however, they are codon optimised for ''E. coli''.
mutable notes: Presence of LacZ alpha peptide upstream of the ORFs coding for the enzymes enables blue- white screening.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J33207
component: BBa_K173003
sequence annotation: BBa_K173003
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K1122676_sequence/1

BBa_J33207
type: Component
persistent identity: component2333624
display id: component2333624
version: 1
top level: BBa_K1122676
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K173003
type: Component
persistent identity: component2333639
display id: component2333639
version: 1
top level: BBa_K1122676
access: public
definition: https://synbiohub.org/public/igem/BBa_K173003/1

BBa_K173003
type: SequenceAnnotation
persistent identity: annotation2333639
display id: annotation2333639
version: 1
top level: BBa_K1122676
location: https://synbiohub.org/public/igem/BBa_K1122676/annotation2333639/range2333639/1
component: BBa_K173003

https://synbiohub.org/public/igem/BBa_K1122676/annotation2333639/range2333639/1
type: Range
persistent identity: range2333639
display id: range2333639
version: 1
direction: direction/reverse
top level: BBa_K1122676
start: 609
end: 3660
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2333624
display id: annotation2333624
version: 1
top level: BBa_K1122676
location: https://synbiohub.org/public/igem/BBa_K1122676/annotation2333624/range2333624/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K1122676/annotation2333624/range2333624/1
type: Range
persistent identity: range2333624
display id: range2333624
version: 1
direction: direction/reverse
top level: BBa_K1122676
start: 1
end: 600
orientation: inline

BBa_M36003
type: ComponentDefinition
persistent identity: BBa M36003
display id: BBa_M36003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glycogen synthase (w/UTR&term)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details.
mutable provenance: Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_M36009
component: BBa_M36010
component: BBa_M36007
sequence annotation: BBa_M36009
sequence annotation: BBa_M36007
sequence annotation: BBa_M36010
sequence: https://synbiohub.org/public/igem/BBa_M36003_sequence/1

BBa_M36009
type: Component
persistent identity: component2117840
display id: component2117840
version: 1
top level: BBa_M36003
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36010
type: Component
persistent identity: component2117842
display id: component2117842
version: 1
top level: BBa_M36003
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36007
type: Component
persistent identity: component2117841
display id: component2117841
version: 1
top level: BBa_M36003
access: public
definition: BBa_M36007

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2117840
display id: annotation2117840
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117840/range2117840/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36003/annotation2117840/range2117840/1
type: Range
persistent identity: range2117840
display id: range2117840
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 1
end: 47
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2117841
display id: annotation2117841
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117841/range2117841/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36003/annotation2117841/range2117841/1
type: Range
persistent identity: range2117841
display id: range2117841
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 48
end: 1481
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2117842
display id: annotation2117842
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117842/range2117842/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36003/annotation2117842/range2117842/1
type: Range
persistent identity: range2117842
display id: range2117842
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 1482
end: 1563
orientation: inline

BBa_K389318
type: ComponentDefinition
persistent identity: BBa K389318
display id: BBa_K389318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a strong constitutive promoter.
mutable notes: strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389004
component: BBa_J23102
component: BBa_B0034
sequence annotation: BBa_J23102
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389318_sequence/1

BBa_K389004
type: Component
persistent identity: component2085590
display id: component2085590
version: 1
top level: BBa_K389318
access: public
definition: luc

BBa_J23102
type: Component
persistent identity: component2085585
display id: component2085585
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_B0034
type: Component
persistent identity: component2085587
display id: component2085587
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2085585
display id: annotation2085585
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
type: Range
persistent identity: range2085585
display id: range2085585
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085590
display id: annotation2085590
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
type: Range
persistent identity: range2085590
display id: range2085590
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085587
display id: annotation2085587
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
type: Range
persistent identity: range2085587
display id: range2085587
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 44
end: 55
orientation: inline

BBa_K552004
type: ComponentDefinition
persistent identity: BBa K552004
display id: BBa_K552004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCD1 Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This DNA sequence codes for the enzyme CCD1, also known as carotenoid cleavage dioxygenase 1. CCD1 cleaves beta-caroten to form two molecules of beta-ionone. This enzyme completes the beta-ionone biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start Codon
sequence annotation: ADH1 Terminator
sequence annotation: Stop Codon
sequence annotation: PGK Promoter
sequence annotation: CCD1
sequence: https://synbiohub.org/public/igem/BBa_K552004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145139
display id: annotation2145139
version: 1
top level: BBa_K552004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145139/range2145139/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145139/range2145139/1
type: Range
persistent identity: range2145139
display id: range2145139
version: 1
direction: direction/forward
top level: BBa_K552004
start: 381
end: 383
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145144
display id: annotation2145144
version: 1
top level: BBa_K552004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145144/range2145144/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145144/range2145144/1
type: Range
persistent identity: range2145144
display id: range2145144
version: 1
direction: direction/forward
top level: BBa_K552004
start: 2012
end: 2141
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145143
display id: annotation2145143
version: 1
top level: BBa_K552004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145143/range2145143/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145143/range2145143/1
type: Range
persistent identity: range2145143
display id: range2145143
version: 1
direction: direction/forward
top level: BBa_K552004
start: 2010
end: 2012
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145140
display id: annotation2145140
version: 1
top level: BBa_K552004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145140/range2145140/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145140/range2145140/1
type: Range
persistent identity: range2145140
display id: range2145140
version: 1
direction: direction/forward
top level: BBa_K552004
start: 1
end: 380
orientation: inline

CCD1
type: SequenceAnnotation
persistent identity: annotation2145142
display id: annotation2145142
version: 1
top level: BBa_K552004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145142/range2145142/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145142/range2145142/1
type: Range
persistent identity: range2145142
display id: range2145142
version: 1
direction: direction/forward
top level: BBa_K552004
start: 381
end: 2012
orientation: inline

TetX
type: ComponentDefinition
persistent identity: BBa K2150101
display id: BBa_K2150101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline resistance protein from Bacteroides fragilis
creator: Zou Zhuoning
created: 2016-10-11T11:00:00Z
modified: 2016-10-16T11:38:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2620_
m user id: 29734
owner id: 29745
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Tet X monooxygenase: Characterization: In vivo qualitative experiements：
mutable notes: protein sequence--gene sequence(coodon preferance) 3 synonynous mutaions
mutable provenance: Tetracycline resistance protein from transposon Tn4351/Tn4400 found in Bacteroides fragilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: tetX
sequence: https://synbiohub.org/public/igem/BBa_K2150101_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2496159
display id: annotation2496159
version: 1
top level: TetX
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2150101/annotation2496159/range2496159/1

https://synbiohub.org/public/igem/BBa_K2150101/annotation2496159/range2496159/1
type: Range
persistent identity: range2496159
display id: range2496159
version: 1
direction: direction/forward
top level: TetX
start: 1171
end: 1173
orientation: inline

tetX
type: SequenceAnnotation
persistent identity: annotation2496011
display id: annotation2496011
version: 1
top level: TetX
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2150101/annotation2496011/range2496011/1

https://synbiohub.org/public/igem/BBa_K2150101/annotation2496011/range2496011/1
type: Range
persistent identity: range2496011
display id: range2496011
version: 1
direction: direction/forward
top level: TetX
start: 1
end: 1170
orientation: inline

BBa_K1373001
type: ComponentDefinition
persistent identity: BBa K1373001
display id: BBa_K1373001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nadE with strong promter and strong RBS
creator: Wenxuan Chen
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1750_
m user id: 0
owner id: 23360
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: nadE gene expression
mutable notes: cloning via omega-PCR
mutable provenance: the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K608002
component: BBa_K1373000
sequence annotation: BBa_K1373000
sequence annotation: BBa_K608002
sequence: https://synbiohub.org/public/igem/BBa_K1373001_sequence/1

BBa_K608002
type: Component
persistent identity: component2422538
display id: component2422538
version: 1
top level: BBa_K1373001
access: public
definition: https://synbiohub.org/public/igem/BBa_K608002/1

BBa_K1373000
type: Component
persistent identity: component2422540
display id: component2422540
version: 1
top level: BBa_K1373001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1373000/1

BBa_K1373000
type: SequenceAnnotation
persistent identity: annotation2422540
display id: annotation2422540
version: 1
top level: BBa_K1373001
location: https://synbiohub.org/public/igem/BBa_K1373001/annotation2422540/range2422540/1
component: BBa_K1373000

https://synbiohub.org/public/igem/BBa_K1373001/annotation2422540/range2422540/1
type: Range
persistent identity: range2422540
display id: range2422540
version: 1
direction: direction/reverse
top level: BBa_K1373001
start: 56
end: 883
orientation: inline

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2422538
display id: annotation2422538
version: 1
top level: BBa_K1373001
location: https://synbiohub.org/public/igem/BBa_K1373001/annotation2422538/range2422538/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1373001/annotation2422538/range2422538/1
type: Range
persistent identity: range2422538
display id: range2422538
version: 1
direction: direction/reverse
top level: BBa_K1373001
start: 1
end: 55
orientation: inline

BBa_K2185003
type: ComponentDefinition
persistent identity: BBa K2185003
display id: BBa_K2185003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Penicillium alpha amylase w/ no promoter
creator: Marissa Sumathipala
created: 2016-09-04T11:00:00Z
modified: 2016-09-15T01:44:50Z
discontinued: false
dominant: false
group u list: _2661_
m user id: 27970
owner id: 27970
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus Penicilium that codes for an alpha amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36). The components of this part are: Kozak Sequence - BBa_K165002 This kozak sequence functions as the ribosome binding site for S.cerevisiae. It is involved in translating the sequence from mRNA to amino acids. Mating Factor Secretion Tag - BBa_K792002 This part is the secretion signal peptide from the S.cerevisiae α-mating factor. This signal peptide directs the secretion of the produced protein, allowing the protein to interact with the environment outside the cell. Fungal Alpha Amylase Coding Sequence - BBa_K2185002 This coding sequence codes for the alpha amylase enzyme from the Pencillium fungus. The enzyme can break alpha bonds linking polysaccharides, thus degrading starch into monosaccharides. ADH1 Terminator - BBa_K392003 This is the terminator region from yeast alcohol dehydrogenase (ADH1) gene.
mutable notes: Removed Kpn and spe sites. Codon optimized for S.cerevisiae.
mutable provenance: Coding sequence - pencilium fungus (sequence from GenBank)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2185002
component: BBa_K792002
component: BBa_K165002
component: BBa_K392003
sequence annotation: BBa_K792002
sequence annotation: BBa_K165002
sequence annotation: BBa_K392003
sequence annotation: BBa_K2185002
sequence: https://synbiohub.org/public/igem/BBa_K2185003_sequence/1

BBa_K2185002
type: Component
persistent identity: component2483512
display id: component2483512
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K2185002/1

BBa_K792002
type: Component
persistent identity: component2483507
display id: component2483507
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K792002/1

BBa_K165002
type: Component
persistent identity: component2483508
display id: component2483508
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K792002
type: Component
persistent identity: component2483510
display id: component2483510
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K792002/1

BBa_K165002
type: Component
persistent identity: component2483505
display id: component2483505
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K165002/1

BBa_K392003
type: Component
persistent identity: component2483511
display id: component2483511
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K392003
type: Component
persistent identity: component2483513
display id: component2483513
version: 1
top level: BBa_K2185003
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2483510
display id: annotation2483510
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483510/range2483510/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483510/range2483510/1
type: Range
persistent identity: range2483510
display id: range2483510
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 113
end: 166
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2483508
display id: annotation2483508
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483508/range2483508/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483508/range2483508/1
type: Range
persistent identity: range2483508
display id: range2483508
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 87
end: 104
orientation: inline

BBa_K792002
type: SequenceAnnotation
persistent identity: annotation2483507
display id: annotation2483507
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483507/range2483507/1
component: BBa_K792002

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483507/range2483507/1
type: Range
persistent identity: range2483507
display id: range2483507
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 27
end: 80
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2483513
display id: annotation2483513
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483513/range2483513/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483513/range2483513/1
type: Range
persistent identity: range2483513
display id: range2483513
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 1583
end: 1711
orientation: inline

BBa_K2185002
type: SequenceAnnotation
persistent identity: annotation2483512
display id: annotation2483512
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483512/range2483512/1
component: BBa_K2185002

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483512/range2483512/1
type: Range
persistent identity: range2483512
display id: range2483512
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 87
end: 1574
orientation: inline

BBa_K165002
type: SequenceAnnotation
persistent identity: annotation2483505
display id: annotation2483505
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483505/range2483505/1
component: BBa_K165002

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483505/range2483505/1
type: Range
persistent identity: range2483505
display id: range2483505
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 1
end: 18
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2483511
display id: annotation2483511
version: 1
top level: BBa_K2185003
location: https://synbiohub.org/public/igem/BBa_K2185003/annotation2483511/range2483511/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K2185003/annotation2483511/range2483511/1
type: Range
persistent identity: range2483511
display id: range2483511
version: 1
direction: direction/reverse
top level: BBa_K2185003
start: 175
end: 303
orientation: inline

BBa_K2023006
type: ComponentDefinition
persistent identity: BBa K2023006
display id: BBa_K2023006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Pu-RBS-Gluc-term
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T01:08:42Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Term-Pu-RBS-Gluc-term (BB3)
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I723020
component: BBa_B0014
component: BBa_K2023009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0014
sequence annotation: BBa_I723020
sequence annotation: BBa_K2023009
sequence: https://synbiohub.org/public/igem/BBa_K2023006_sequence/1

BBa_I723020
type: Component
persistent identity: component2518736
display id: component2518736
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0014
type: Component
persistent identity: component2518734
display id: component2518734
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: Component
persistent identity: component2518747
display id: component2518747
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K2023009
type: Component
persistent identity: component2518740
display id: component2518740
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023009/1

BBa_B0034
type: Component
persistent identity: component2518738
display id: component2518738
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518738
display id: annotation2518738
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518738/range2518738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518738/range2518738/1
type: Range
persistent identity: range2518738
display id: range2518738
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 432
end: 443
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518734
display id: annotation2518734
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518734/range2518734/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518734/range2518734/1
type: Range
persistent identity: range2518734
display id: range2518734
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 1
end: 95
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518736
display id: annotation2518736
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518736/range2518736/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518736/range2518736/1
type: Range
persistent identity: range2518736
display id: range2518736
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 104
end: 423
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518747
display id: annotation2518747
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518747/range2518747/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518747/range2518747/1
type: Range
persistent identity: range2518747
display id: range2518747
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 1019
end: 1113
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2518740
display id: annotation2518740
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518740/range2518740/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518740/range2518740/1
type: Range
persistent identity: range2518740
display id: range2518740
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 450
end: 1010
orientation: inline

BBa_K567014
type: ComponentDefinition
persistent identity: BBa K567014
display id: BBa_K567014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-metGM
creator: You Wang
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and have used error-prone PCR to amplify the metG. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LB Kana plate.
mutable notes: Overlapping PCR is used
mutable provenance: metG from E.coli. Kana gene derived from pET28a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: MetRS(mutated)
sequence annotation: T7 promoter
sequence annotation: lac operator
sequence annotation: rbs
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K567014_sequence/1

MetRS(mutated)
type: SequenceAnnotation
persistent identity: annotation2152910
display id: annotation2152910
version: 1
top level: BBa_K567014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567014/annotation2152910/range2152910/1

https://synbiohub.org/public/igem/BBa_K567014/annotation2152910/range2152910/1
type: Range
persistent identity: range2152910
display id: range2152910
version: 1
direction: direction/forward
top level: BBa_K567014
start: 89
end: 2122
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150826
display id: annotation2150826
version: 1
top level: BBa_K567014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567014/annotation2150826/range2150826/1

https://synbiohub.org/public/igem/BBa_K567014/annotation2150826/range2150826/1
type: Range
persistent identity: range2150826
display id: range2150826
version: 1
direction: direction/forward
top level: BBa_K567014
start: 2
end: 18
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150827
display id: annotation2150827
version: 1
top level: BBa_K567014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567014/annotation2150827/range2150827/1

https://synbiohub.org/public/igem/BBa_K567014/annotation2150827/range2150827/1
type: Range
persistent identity: range2150827
display id: range2150827
version: 1
direction: direction/forward
top level: BBa_K567014
start: 21
end: 44
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150828
display id: annotation2150828
version: 1
top level: BBa_K567014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567014/annotation2150828/range2150828/1

https://synbiohub.org/public/igem/BBa_K567014/annotation2150828/range2150828/1
type: Range
persistent identity: range2150828
display id: range2150828
version: 1
direction: direction/forward
top level: BBa_K567014
start: 75
end: 81
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152915
display id: annotation2152915
version: 1
top level: BBa_K567014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K567014/annotation2152915/range2152915/1

https://synbiohub.org/public/igem/BBa_K567014/annotation2152915/range2152915/1
type: Range
persistent identity: range2152915
display id: range2152915
version: 1
direction: direction/forward
top level: BBa_K567014
start: 2209
end: 2256
orientation: inline

BBa_K863005
type: ComponentDefinition
persistent identity: BBa K863005
display id: BBa_K863005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: E.coli laccase ORF with T7, RBS and HIS tag
mutable notes: TEST
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: ecol
sequence annotation: ATG
sequence annotation: 6x HIS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K863005_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184564
display id: annotation2184564
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1
type: Range
persistent identity: range2184564
display id: range2184564
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1600
end: 1602
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184561
display id: annotation2184561
version: 1
top level: BBa_K863005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1
type: Range
persistent identity: range2184561
display id: range2184561
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1
end: 23
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184563
display id: annotation2184563
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1
type: Range
persistent identity: range2184563
display id: range2184563
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1603
end: 1605
orientation: inline

ecol
type: SequenceAnnotation
persistent identity: annotation2184566
display id: annotation2184566
version: 1
top level: BBa_K863005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1
type: Range
persistent identity: range2184566
display id: range2184566
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 1581
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184562
display id: annotation2184562
version: 1
top level: BBa_K863005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1
type: Range
persistent identity: range2184562
display id: range2184562
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 36
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184565
display id: annotation2184565
version: 1
top level: BBa_K863005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1
type: Range
persistent identity: range2184565
display id: range2184565
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1582
end: 1599
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187301
display id: annotation2187301
version: 1
top level: BBa_K863005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1
type: Range
persistent identity: range2187301
display id: range2187301
version: 1
direction: direction/forward
top level: BBa_K863005
start: 25
end: 30
orientation: inline

BBa_K103009
type: ComponentDefinition
persistent identity: BBa K103009
display id: BBa_K103009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: linker fused to alpha fragment of TEM beta-lactamase
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: alpha
sequence annotation: linker
sequence annotation: SacI
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K103009_sequence/1

alpha
type: SequenceAnnotation
persistent identity: annotation1986313
display id: annotation1986313
version: 1
top level: BBa_K103009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103009/annotation1986313/range1986313/1

https://synbiohub.org/public/igem/BBa_K103009/annotation1986313/range1986313/1
type: Range
persistent identity: range1986313
display id: range1986313
version: 1
direction: direction/forward
top level: BBa_K103009
start: 60
end: 578
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1986311
display id: annotation1986311
version: 1
top level: BBa_K103009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103009/annotation1986311/range1986311/1

https://synbiohub.org/public/igem/BBa_K103009/annotation1986311/range1986311/1
type: Range
persistent identity: range1986311
display id: range1986311
version: 1
direction: direction/forward
top level: BBa_K103009
start: 1
end: 59
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1986312
display id: annotation1986312
version: 1
top level: BBa_K103009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103009/annotation1986312/range1986312/1

https://synbiohub.org/public/igem/BBa_K103009/annotation1986312/range1986312/1
type: Range
persistent identity: range1986312
display id: range1986312
version: 1
direction: direction/forward
top level: BBa_K103009
start: 2
end: 7
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1986314
display id: annotation1986314
version: 1
top level: BBa_K103009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K103009/annotation1986314/range1986314/1

https://synbiohub.org/public/igem/BBa_K103009/annotation1986314/range1986314/1
type: Range
persistent identity: range1986314
display id: range1986314
version: 1
direction: direction/forward
top level: BBa_K103009
start: 576
end: 578
orientation: inline

PPK
type: ComponentDefinition
persistent identity: BBa K986000
display id: BBa_K986000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a PPK Coding Sequence which can remove phosphate from water
creator: Kang Li
created: 2013-04-19T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1283_
m user id: 0
owner id: 14783
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a PPK CDS which is created by Jack Jia from Shenzhen_SFLS and with Eric's great help. The polyphosphatase then degrades phosphates present when there is too much phosphorus in water. We use it as a CDS in our project.
mutable notes: Nothing. Everything goes on well during our research.
mutable provenance: E-coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K986000_sequence/1

BBa_I716254
type: ComponentDefinition
persistent identity: BBa I716254
display id: BBa_I716254
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal aminopeptidase (methionine aminopeptidase ??? map gene)
creator: Vaibhavi Umesh
created: 2007-06-05T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 1696
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: basic part for the chaperone/toxicity device
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}} N-terminal aminopeptidase (methionine aminopeptidase ??? map gene) Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the N-terminal methionine aminopeptidase (map) open reading frame from Salmonella typhimurium LT2 PCR VU07F.map/VU08R.map on methionine aminopeptidase (817 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU3 ------------------------------------- VU07F.map ccaaaAGATCTatggctatctcaatcaagacccc VU08R.map gctagCTCGAGttaGGATCCttattcgtcgtgcgagattatcg
mutable provenance: Escherichia coli K-12 MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716254_sequence/1

BBa_K777121
type: ComponentDefinition
persistent identity: BBa K777121
display id: BBa_K777121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH
creator: Flash Coli
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3' ** Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YhjH coding sequence
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K777121_sequence/1

YhjH coding sequence
type: SequenceAnnotation
persistent identity: annotation2184528
display id: annotation2184528
version: 1
top level: BBa_K777121
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184528/range2184528/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184528/range2184528/1
type: Range
persistent identity: range2184528
display id: range2184528
version: 1
direction: direction/forward
top level: BBa_K777121
start: 1
end: 768
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2184529
display id: annotation2184529
version: 1
top level: BBa_K777121
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184529/range2184529/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184529/range2184529/1
type: Range
persistent identity: range2184529
display id: range2184529
version: 1
direction: direction/forward
top level: BBa_K777121
start: 1
end: 1
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2184530
display id: annotation2184530
version: 1
top level: BBa_K777121
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777121/annotation2184530/range2184530/1

https://synbiohub.org/public/igem/BBa_K777121/annotation2184530/range2184530/1
type: Range
persistent identity: range2184530
display id: range2184530
version: 1
direction: direction/forward
top level: BBa_K777121
start: 768
end: 768
orientation: inline

Red
type: ComponentDefinition
persistent identity: BBa K525499
display id: BBa_K525499
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADP+ Oxidoreductase from Escherichia coli
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NADP+ Oxidoreductase
mutable notes: PCR from E.coli
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: Reductase
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K525499_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2130006
display id: annotation2130006
version: 1
top level: Red
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130006/range2130006/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130006/range2130006/1
type: Range
persistent identity: range2130006
display id: range2130006
version: 1
direction: direction/forward
top level: Red
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2130009
display id: annotation2130009
version: 1
top level: Red
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130009/range2130009/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130009/range2130009/1
type: Range
persistent identity: range2130009
display id: range2130009
version: 1
direction: direction/forward
top level: Red
start: 757
end: 759
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2130007
display id: annotation2130007
version: 1
top level: Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130007/range2130007/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130007/range2130007/1
type: Range
persistent identity: range2130007
display id: range2130007
version: 1
direction: direction/forward
top level: Red
start: 4
end: 9
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2130005
display id: annotation2130005
version: 1
top level: Red
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130005/range2130005/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130005/range2130005/1
type: Range
persistent identity: range2130005
display id: range2130005
version: 1
direction: direction/forward
top level: Red
start: 1
end: 759
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130008
display id: annotation2130008
version: 1
top level: Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130008/range2130008/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130008/range2130008/1
type: Range
persistent identity: range2130008
display id: range2130008
version: 1
direction: direction/forward
top level: Red
start: 751
end: 756
orientation: inline

BBa_K1072007
type: ComponentDefinition
persistent identity: BBa K1072007
display id: BBa_K1072007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM
creator: Junjie zhang, Yichong wang, Zewei yan, Rui chen and Xuenian chen
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_K1072007 is a composite divece, including LuxR, LuxI, GFP and α-ALS genes. LuxR is under control of a constitutive promotor, Lux pI. Produce, LuxR, forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreas the transcriptional rate of the lux pL promoter. The other genes are under control of this lux pR promoter. The generator, Lux pR+RBS+LuxI+B0015, express AHL which will combine with LuxR. GFP also is under control of Lux pR, presenting as reporter. α-ALS，as our desire produce, will transcrip activated by LuxR and AHL complex. If co-transforming with B0015 , this system will transcript α-ALS periodically. Finally, this part just is the combination of BBa_K1072000 and BBa_K1072006 .
mutable notes: -
mutable provenance: Some part get from registry and α-ALS gene originate from Lactococcus lactis, respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072006
component: BBa_K1072000
sequence annotation: BBa_K1072000
sequence annotation: BBa_K1072006
sequence: https://synbiohub.org/public/igem/BBa_K1072007_sequence/1

BBa_K1072006
type: Component
persistent identity: component2378014
display id: component2378014
version: 1
top level: BBa_K1072007
access: public
definition: BBa_K1072006

BBa_K1072000
type: Component
persistent identity: component2377998
display id: component2377998
version: 1
top level: BBa_K1072007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072000/1

BBa_K1072000
type: SequenceAnnotation
persistent identity: annotation2377998
display id: annotation2377998
version: 1
top level: BBa_K1072007
location: https://synbiohub.org/public/igem/BBa_K1072007/annotation2377998/range2377998/1
component: BBa_K1072000

https://synbiohub.org/public/igem/BBa_K1072007/annotation2377998/range2377998/1
type: Range
persistent identity: range2377998
display id: range2377998
version: 1
direction: direction/reverse
top level: BBa_K1072007
start: 1
end: 2910
orientation: inline

BBa_K1072006
type: SequenceAnnotation
persistent identity: annotation2378014
display id: annotation2378014
version: 1
top level: BBa_K1072007
location: https://synbiohub.org/public/igem/BBa_K1072007/annotation2378014/range2378014/1
component: BBa_K1072006

https://synbiohub.org/public/igem/BBa_K1072007/annotation2378014/range2378014/1
type: Range
persistent identity: range2378014
display id: range2378014
version: 1
direction: direction/reverse
top level: BBa_K1072007
start: 2919
end: 4801
orientation: inline

BBa_K515001
type: ComponentDefinition
persistent identity: BBa K515001
display id: BBa_K515001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IaaH - Indoleacetamide hydrolyase
creator: Atipat Patharagulpong
created: 2011-08-31T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: IaaH encodes Indoleacetimide hydrolase which catalyses Indolacetimide to IAA
mutable notes: -
mutable provenance: genomic sequence of
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: IaaH
sequence annotation: insulator
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K515001_sequence/1

IaaH
type: SequenceAnnotation
persistent identity: annotation2125781
display id: annotation2125781
version: 1
top level: BBa_K515001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515001/annotation2125781/range2125781/1

https://synbiohub.org/public/igem/BBa_K515001/annotation2125781/range2125781/1
type: Range
persistent identity: range2125781
display id: range2125781
version: 1
direction: direction/forward
top level: BBa_K515001
start: 39
end: 1382
orientation: inline

insulator
type: SequenceAnnotation
persistent identity: annotation2125779
display id: annotation2125779
version: 1
top level: BBa_K515001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K515001/annotation2125779/range2125779/1

https://synbiohub.org/public/igem/BBa_K515001/annotation2125779/range2125779/1
type: Range
persistent identity: range2125779
display id: range2125779
version: 1
direction: direction/forward
top level: BBa_K515001
start: 1
end: 15
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2125780
display id: annotation2125780
version: 1
top level: BBa_K515001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K515001/annotation2125780/range2125780/1

https://synbiohub.org/public/igem/BBa_K515001/annotation2125780/range2125780/1
type: Range
persistent identity: range2125780
display id: range2125780
version: 1
direction: direction/forward
top level: BBa_K515001
start: 16
end: 38
orientation: inline

B-carotene
type: ComponentDefinition
persistent identity: BBa K343006
display id: BBa_K343006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expresses B-carotene monooxygenase on a constitutive promotor
creator: Christian Kurtzhals
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
mutable notes: The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
mutable provenance: The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_K343001
component: BBa_B0034
component: BBa_B0010
component: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343006_sequence/1

BBa_B0012
type: Component
persistent identity: component2085991
display id: component2085991
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K343001
type: Component
persistent identity: component2085988
display id: component2085988
version: 1
top level: B-carotene
access: public
definition: ninaB

BBa_B0034
type: Component
persistent identity: component2085984
display id: component2085984
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2085989
display id: component2085989
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component2085978
display id: component2085978
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085989
display id: annotation2085989
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
type: Range
persistent identity: range2085989
display id: range2085989
version: 1
direction: direction/forward
top level: B-carotene
start: 1952
end: 2031
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085991
display id: annotation2085991
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
type: Range
persistent identity: range2085991
display id: range2085991
version: 1
direction: direction/forward
top level: B-carotene
start: 2040
end: 2080
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2085978
display id: annotation2085978
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
type: Range
persistent identity: range2085978
display id: range2085978
version: 1
direction: direction/forward
top level: B-carotene
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085984
display id: annotation2085984
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
type: Range
persistent identity: range2085984
display id: range2085984
version: 1
direction: direction/reverse
top level: B-carotene
start: 63
end: 74
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2085988
display id: annotation2085988
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
type: Range
persistent identity: range2085988
display id: range2085988
version: 1
direction: direction/reverse
top level: B-carotene
start: 81
end: 1943
orientation: inline

BBa_K133071
type: ComponentDefinition
persistent identity: BBa K133071
display id: BBa_K133071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UreB
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cloned into pSB1AK3 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K133071_sequence/1

BBa_K1331003
type: ComponentDefinition
persistent identity: BBa K1331003
display id: BBa_K1331003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
mutable notes: It includes both the complete coding sequence of RhlI and part of noncoding sequence between RhlR and RhlI gene.
mutable provenance: It comes from the genomic sequence of P.aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: RhlI
sequence: https://synbiohub.org/public/igem/BBa_K1331003_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427981
display id: annotation2427981
version: 1
top level: BBa_K1331003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1
type: Range
persistent identity: range2427981
display id: range2427981
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 1
end: 113
orientation: inline

RhlI
type: SequenceAnnotation
persistent identity: annotation2427973
display id: annotation2427973
version: 1
top level: BBa_K1331003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1
type: Range
persistent identity: range2427973
display id: range2427973
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 114
end: 719
orientation: inline

BBa_K1072004
type: ComponentDefinition
persistent identity: BBa K1072004
display id: BBa_K1072004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alpha-ALS Coding Region
creator: Zewei yan, Yichong wang and Junjie zhang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072004 is the conding region of α-acetolactate synthetase(α-ALS).
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: alpha-ALS
sequence: https://synbiohub.org/public/igem/BBa_K1072004_sequence/1

alpha-ALS
type: SequenceAnnotation
persistent identity: annotation2335232
display id: annotation2335232
version: 1
top level: BBa_K1072004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072004/annotation2335232/range2335232/1

https://synbiohub.org/public/igem/BBa_K1072004/annotation2335232/range2335232/1
type: Range
persistent identity: range2335232
display id: range2335232
version: 1
direction: direction/forward
top level: BBa_K1072004
start: 1
end: 1665
orientation: inline

HyaB -> E.
type: ComponentDefinition
persistent identity: BBa K1958002
display id: BBa_K1958002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaB -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HyaB is the large subunit of hydrogenase 1 (Hyd-1) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structual subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaB is the second gene of hya operon, sharing the first few base pairs with hyaA and the last few base pairs with hyaC. This large subunit of this [Ni-Fe] hydrogenase contains the active site. The large subunit exhibit sequence homologies to subunits of NADH: ubiquinone oxidoreductase (Complex I).
mutable notes: None.
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: TAA stop codon
sequence annotation: HyaB coding region
sequence: https://synbiohub.org/public/igem/BBa_K1958002_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493856
display id: annotation2493856
version: 1
top level: HyaB -> E.
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958002/annotation2493856/range2493856/1

https://synbiohub.org/public/igem/BBa_K1958002/annotation2493856/range2493856/1
type: Range
persistent identity: range2493856
display id: range2493856
version: 1
direction: direction/forward
top level: HyaB -> E.
start: 1
end: 3
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2493858
display id: annotation2493858
version: 1
top level: HyaB -> E.
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958002/annotation2493858/range2493858/1

https://synbiohub.org/public/igem/BBa_K1958002/annotation2493858/range2493858/1
type: Range
persistent identity: range2493858
display id: range2493858
version: 1
direction: direction/forward
top level: HyaB -> E.
start: 1792
end: 1794
orientation: inline

HyaB coding region
type: SequenceAnnotation
persistent identity: annotation2493857
display id: annotation2493857
version: 1
top level: HyaB -> E.
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958002/annotation2493857/range2493857/1

https://synbiohub.org/public/igem/BBa_K1958002/annotation2493857/range2493857/1
type: Range
persistent identity: range2493857
display id: range2493857
version: 1
direction: direction/forward
top level: HyaB -> E.
start: 1
end: 1794
orientation: inline

BBa_K863010
type: ComponentDefinition
persistent identity: BBa K863010
display id: BBa_K863010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x HIS
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863010_sequence/1

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184733
display id: annotation2184733
version: 1
top level: BBa_K863010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1

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type: Range
persistent identity: range2184733
display id: range2184733
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1420
end: 1437
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184671
display id: annotation2184671
version: 1
top level: BBa_K863010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1
type: Range
persistent identity: range2184671
display id: range2184671
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 36
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184782
display id: annotation2184782
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1
type: Range
persistent identity: range2184782
display id: range2184782
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1438
end: 1440
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184708
display id: annotation2184708
version: 1
top level: BBa_K863010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1
type: Range
persistent identity: range2184708
display id: range2184708
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1
end: 23
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2184732
display id: annotation2184732
version: 1
top level: BBa_K863010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1
type: Range
persistent identity: range2184732
display id: range2184732
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 1419
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184783
display id: annotation2184783
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1
type: Range
persistent identity: range2184783
display id: range2184783
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1441
end: 1443
orientation: inline

BBa_K103014
type: ComponentDefinition
persistent identity: BBa K103014
display id: BBa_K103014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA_linker_alpha_linker
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K103006
component: BBa_K103008
sequence annotation: BBa_K103006
sequence annotation: BBa_K103008
sequence: https://synbiohub.org/public/igem/BBa_K103014_sequence/1

BBa_K103006
type: Component
persistent identity: component1993510
display id: component1993510
version: 1
top level: BBa_K103014
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103008
type: Component
persistent identity: component1993514
display id: component1993514
version: 1
top level: BBa_K103014
access: public
definition: https://synbiohub.org/public/igem/BBa_K103008/1

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation1993510
display id: annotation1993510
version: 1
top level: BBa_K103014
location: https://synbiohub.org/public/igem/BBa_K103014/annotation1993510/range1993510/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K103014/annotation1993510/range1993510/1
type: Range
persistent identity: range1993510
display id: range1993510
version: 1
direction: direction/reverse
top level: BBa_K103014
start: 1
end: 464
orientation: inline

BBa_K103008
type: SequenceAnnotation
persistent identity: annotation1993514
display id: annotation1993514
version: 1
top level: BBa_K103014
location: https://synbiohub.org/public/igem/BBa_K103014/annotation1993514/range1993514/1
component: BBa_K103008

https://synbiohub.org/public/igem/BBa_K103014/annotation1993514/range1993514/1
type: Range
persistent identity: range1993514
display id: range1993514
version: 1
direction: direction/reverse
top level: BBa_K103014
start: 465
end: 1049
orientation: inline

BBa_M36006
type: ComponentDefinition
persistent identity: BBa M36006
display id: BBa_M36006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glucose1phosphate adenylyltransferase
creator: Jesse Palmer, Elliot Lui
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details.
mutable notes: This mutation results in greater glycogen production compared to WT.
mutable provenance: The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->T
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_M36006_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation2119202
display id: annotation2119202
version: 1
top level: BBa_M36006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119202/range2119202/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119202/range2119202/1
type: Range
persistent identity: range2119202
display id: range2119202
version: 1
direction: direction/forward
top level: BBa_M36006
start: 199
end: 199
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119203
display id: annotation2119203
version: 1
top level: BBa_M36006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119203/range2119203/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119203/range2119203/1
type: Range
persistent identity: range2119203
display id: range2119203
version: 1
direction: direction/forward
top level: BBa_M36006
start: 1294
end: 1296
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119064
display id: annotation2119064
version: 1
top level: BBa_M36006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119064/range2119064/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119064/range2119064/1
type: Range
persistent identity: range2119064
display id: range2119064
version: 1
direction: direction/forward
top level: BBa_M36006
start: 1
end: 3
orientation: inline

BBa_K808026
type: ComponentDefinition
persistent identity: BBa K808026
display id: BBa_K808026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pNB-Est 13: Esterase PET cleaving enzyme
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The pNB-ESterase 13 is a mutant derived from pNB-Esterase from Bacillus licheniformis. It showed the highest affinity and activity towards PET.
mutable notes: -
mutable provenance: Our team obtained it from Henkel GmbH on an pET26b(+) vector: Fakult, M., & Enzymtechnologie, M. (2006). Isolierung, Identifizierung und biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808026_sequence/1

BBa_K1331005
type: ComponentDefinition
persistent identity: BBa K1331005
display id: BBa_K1331005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlABRI operon genes from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1331003
component: BBa_K1331004
component: BBa_K1331001
component: BBa_K1331002
sequence annotation: BBa_K1331001
sequence annotation: BBa_K1331002
sequence annotation: BBa_K1331003
sequence annotation: BBa_K1331004
sequence: https://synbiohub.org/public/igem/BBa_K1331005_sequence/1

BBa_K1331003
type: Component
persistent identity: component2421130
display id: component2421130
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331003

BBa_K1331004
type: Component
persistent identity: component2421125
display id: component2421125
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331004

BBa_K1331001
type: Component
persistent identity: component2421124
display id: component2421124
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331001

BBa_K1331002
type: Component
persistent identity: component2421128
display id: component2421128
version: 1
top level: BBa_K1331005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1331002/1

BBa_K1331001
type: SequenceAnnotation
persistent identity: annotation2421124
display id: annotation2421124
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421124/range2421124/1
component: BBa_K1331001

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421124/range2421124/1
type: Range
persistent identity: range2421124
display id: range2421124
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 1
end: 947
orientation: inline

BBa_K1331002
type: SequenceAnnotation
persistent identity: annotation2421128
display id: annotation2421128
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421128/range2421128/1
component: BBa_K1331002

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421128/range2421128/1
type: Range
persistent identity: range2421128
display id: range2421128
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 2271
end: 3167
orientation: inline

BBa_K1331003
type: SequenceAnnotation
persistent identity: annotation2421130
display id: annotation2421130
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421130/range2421130/1
component: BBa_K1331003

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421130/range2421130/1
type: Range
persistent identity: range2421130
display id: range2421130
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 3176
end: 3894
orientation: inline

BBa_K1331004
type: SequenceAnnotation
persistent identity: annotation2421125
display id: annotation2421125
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421125/range2421125/1
component: BBa_K1331004

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421125/range2421125/1
type: Range
persistent identity: range2421125
display id: range2421125
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 956
end: 2262
orientation: inline

BBa_I716211
type: ComponentDefinition
persistent identity: BBa I716211
display id: BBa_I716211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barnase active peptide
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Barnase active peptide (no start codon)
mutable notes: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
mutable provenance: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716211_sequence/1

BBa_K357005
type: ComponentDefinition
persistent identity: BBa K357005
display id: BBa_K357005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-oxoadipate CoA-transferase, subunit A
creator: Elena Pasko
created: 2010-06-27T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _530_
m user id: 0
owner id: 7009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Catalyzes the following reaction: succinyl-CoA + 3-oxoadipate <<-->> succinate + 3-oxoadipyl-CoA This enzyme consists of a alpha and beta subunit. It is involved in benzoate degradation via hydroxylation.
mutable notes: No Pstl Restriction Sites GC=64%, AT=36%, ORF_2 rf(4)(1,696)<<<, ORF_1 rf(1)(67,696)>>>
mutable provenance: SOURCE Pseudomonas putida KT2440 ORGANISM Pseudomonas putida KT2440 Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 696) AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L., Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R., Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P., Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T., Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J., Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K., Duesterhoft,A., Tummler,B. and Fraser,C. TITLE Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440 JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon
sequence annotation: NgoMIV Site Removed
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K357005_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2071664
display id: annotation2071664
version: 1
top level: BBa_K357005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K357005/annotation2071664/range2071664/1

https://synbiohub.org/public/igem/BBa_K357005/annotation2071664/range2071664/1
type: Range
persistent identity: range2071664
display id: range2071664
version: 1
direction: direction/forward
top level: BBa_K357005
start: 694
end: 696
orientation: inline

NgoMIV Site Removed
type: SequenceAnnotation
persistent identity: annotation2072350
display id: annotation2072350
version: 1
top level: BBa_K357005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K357005/annotation2072350/range2072350/1

https://synbiohub.org/public/igem/BBa_K357005/annotation2072350/range2072350/1
type: Range
persistent identity: range2072350
display id: range2072350
version: 1
direction: direction/forward
top level: BBa_K357005
start: 277
end: 282
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2071663
display id: annotation2071663
version: 1
top level: BBa_K357005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K357005/annotation2071663/range2071663/1

https://synbiohub.org/public/igem/BBa_K357005/annotation2071663/range2071663/1
type: Range
persistent identity: range2071663
display id: range2071663
version: 1
direction: direction/forward
top level: BBa_K357005
start: 1
end: 3
orientation: inline

BBa_K567005
type: ComponentDefinition
persistent identity: BBa K567005
display id: BBa_K567005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbla-Luc-4AGG
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: β-lactamase promoter-Luciferase with four AGG-codon insertions. This biobrick is constructed by putting modified enzyme luciferase under constituitive promoter β-lactamase promoter. 4 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg)(BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin and 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: Point mutation is used to obtain this part from wild type
mutable provenance: β-lactamase promoter is derived from pUC18 β-lactamase operon. Luciferase with two AGG-codon insertions from BBa_K567004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Pbla
sequence annotation: luciferase-4AGG
sequence annotation: 4AGG
sequence annotation: ATG
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K567005_sequence/1

Pbla
type: SequenceAnnotation
persistent identity: annotation2152727
display id: annotation2152727
version: 1
top level: BBa_K567005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567005/annotation2152727/range2152727/1

https://synbiohub.org/public/igem/BBa_K567005/annotation2152727/range2152727/1
type: Range
persistent identity: range2152727
display id: range2152727
version: 1
direction: direction/forward
top level: BBa_K567005
start: 217
end: 244
orientation: inline

luciferase-4AGG
type: SequenceAnnotation
persistent identity: annotation2150760
display id: annotation2150760
version: 1
top level: BBa_K567005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567005/annotation2150760/range2150760/1

https://synbiohub.org/public/igem/BBa_K567005/annotation2150760/range2150760/1
type: Range
persistent identity: range2150760
display id: range2150760
version: 1
direction: direction/forward
top level: BBa_K567005
start: 288
end: 1958
orientation: inline

4AGG
type: SequenceAnnotation
persistent identity: annotation2150759
display id: annotation2150759
version: 1
top level: BBa_K567005
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567005/annotation2150759/range2150759/1

https://synbiohub.org/public/igem/BBa_K567005/annotation2150759/range2150759/1
type: Range
persistent identity: range2150759
display id: range2150759
version: 1
direction: direction/forward
top level: BBa_K567005
start: 290
end: 302
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150758
display id: annotation2150758
version: 1
top level: BBa_K567005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567005/annotation2150758/range2150758/1

https://synbiohub.org/public/igem/BBa_K567005/annotation2150758/range2150758/1
type: Range
persistent identity: range2150758
display id: range2150758
version: 1
direction: direction/forward
top level: BBa_K567005
start: 288
end: 290
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154562
display id: annotation2154562
version: 1
top level: BBa_K567005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567005/annotation2154562/range2154562/1

https://synbiohub.org/public/igem/BBa_K567005/annotation2154562/range2154562/1
type: Range
persistent identity: range2154562
display id: range2154562
version: 1
direction: direction/forward
top level: BBa_K567005
start: 278
end: 282
orientation: inline

ArfB
type: ComponentDefinition
persistent identity: BBa K1360000
display id: BBa_K1360000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alpha-L-arabinofuranosidase ArfB
creator: Jiang Liang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1736_
m user id: 0
owner id: 19709
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: encodes alpha-L-arabinofuranosidase ArfB from Clostridium stercorarium DSM8532, codon optimized for E.coli
mutable notes: codon optimized for E.coli
mutable provenance: from whole genome sequenceing of Clostridium stercorarium DSM8532, codon optimized for E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1360000_sequence/1

BBa_K1720000
type: ComponentDefinition
persistent identity: BBa K1720000
display id: BBa_K1720000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human guanylate cyclase1,soluble, alpha 3 unit
creator: YuanbinCui
created: 2015-08-08T11:00:00Z
modified: 2015-08-31T03:42:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: Soluble guanylate cyclases(sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It is important for smooth muscle relaxation in the cardiovascular system. sGC contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs.There are several different isoforms of sGC subunit ,but the cardiovascular system abundant with alpha 3 unit and beta 3 unit.
mutable notes: This subunit coding part with a GFP reporter was then transfected into HEK293 cells and in vivo green fluorescence signal was observed under fluorescence microscope. After that we used lentiviral vector to transfected alpha 3 unit and beta 3 unit to HEK293 cells together. Since soluble guanylate cyclases (sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP(cGMP) and pyrophosphate. The level of cGMP will be up regulated.The positive control was HEK293 cells that treat with Sodium Nitroprusside ,a NO donator that activate sGC and up regulate the level of cGMP. A negative control was made by transfecting an empty vector that does not contain sGC subunit. We used Elisa to detect cGMP level.
mutable provenance: http://www.ncbi.nlm.nih.gov/gene/2982
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1720000_sequence/1

Biosurfact
type: ComponentDefinition
persistent identity: BBa K653000
display id: BBa_K653000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Re-designing "The Biosurfactator" The first Central American BioBrick
creator: Team Panama 2011
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (RhlAB). This is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid, which is a biomolecule with surfactant properties, but the natural RhlAB gene has illegal restriction sites that make it incompatible with the Assembly Standard Protocol 10, specifically the Pstl1 restriction sites. The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year???s iGEM project we failed in producing the rhamnolipid compound into our E. coli based-factory, because our last year???s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part BBa_K424018 (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone. As scientist and iGEMers, we have the responsibility to deliver a functional BioBrick, thus according to iGEM???s competition new slogan, ???Quality not Quantity???, we have embraced the challenge of re-designing and re-building the same BioBrick part from last year in order to comply this new standard based on quality. How to use it as a BioBrick? : We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
mutable notes: The RhlAB gene complex has shown illegal restriction sites according to the assembly standard protocol 10. We faced this problem by applying the mutagenesis protocol that consist in doing silent mutations that change the nucleotides of our rhlAB gene that are cut by the Pstl restriction enzyme. This will give us a standardized coding sequence of the rhlAB gene compatible with the Assembly Standard protocol 10 and a new BioBrick part for the Registry.
mutable provenance: Pseudomonas aeruginosa from INDICASAT lab. and the genomic sequence of RhlAB was reviwed in genBank (ID 878955 and 878954) http://www.ncbi.nlm.nih.gov/nuccore/L28170.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rhamnosyltranferase A
sequence annotation: Silent Mutation
sequence annotation: Rhamnosyltransferase B
sequence annotation: Primer
sequence annotation: Rhamnosyltransferase gene complex 1
sequence annotation: BBa_ K653000 RhlAB Panama
sequence: https://synbiohub.org/public/igem/BBa_K653000_sequence/1

Rhamnosyltranferase A
type: SequenceAnnotation
persistent identity: annotation2142964
display id: annotation2142964
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1
type: Range
persistent identity: range2142964
display id: range2142964
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 922
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142965
display id: annotation2142965
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1
type: Range
persistent identity: range2142965
display id: range2142965
version: 1
direction: direction/forward
top level: Biosurfact
start: 754
end: 759
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142967
display id: annotation2142967
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1
type: Range
persistent identity: range2142967
display id: range2142967
version: 1
direction: direction/forward
top level: Biosurfact
start: 1777
end: 1782
orientation: inline

Rhamnosyltransferase B
type: SequenceAnnotation
persistent identity: annotation2142966
display id: annotation2142966
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1
type: Range
persistent identity: range2142966
display id: range2142966
version: 1
direction: direction/forward
top level: Biosurfact
start: 988
end: 2268
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142968
display id: annotation2142968
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1
type: Range
persistent identity: range2142968
display id: range2142968
version: 1
direction: direction/forward
top level: Biosurfact
start: 1891
end: 1896
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142962
display id: annotation2142962
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1
type: Range
persistent identity: range2142962
display id: range2142962
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 19
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142969
display id: annotation2142969
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1
type: Range
persistent identity: range2142969
display id: range2142969
version: 1
direction: direction/reverse
top level: Biosurfact
start: 2296
end: 2314
orientation: inline

Rhamnosyltransferase gene complex 1
type: SequenceAnnotation
persistent identity: annotation2142963
display id: annotation2142963
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1
type: Range
persistent identity: range2142963
display id: range2142963
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 2234
orientation: inline

BBa_ K653000 RhlAB Panama
type: SequenceAnnotation
persistent identity: annotation2142961
display id: annotation2142961
version: 1
top level: Biosurfact
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1
type: Range
persistent identity: range2142961
display id: range2142961
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 2314
orientation: inline

BBa_K1199042
type: ComponentDefinition
persistent identity: BBa K1199042
display id: BBa_K1199042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LinB 1,3,4,6-tetrachloro-1,4-cyclohexadiene->2,5-dichloro-2,5-cyclohexadiene-1,4-diol
creator: Tian Wang
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1513_
m user id: 0
owner id: 16962
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: LinB is cofactor-independent dehalogenase from Pseudomonas paucimobilis UT26,and can do furthur degration of gamma-HCH after LinA's work to purify water .
mutable notes: No restriction sites contained
mutable provenance: LinB is from Pseudomonas paucimobilis UT26
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1199042_sequence/1

PyrF
type: ComponentDefinition
persistent identity: BBa T9150
display id: BBa_T9150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: orotidine 5
creator: Jessica Harpole
created: 2005-08-22T11:00:00Z
modified: 2015-05-08T01:14:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.
mutable notes: Four silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. The EcoRI site was eliminated at base 39 with a G -> A mutation. PstI sites were eliminated at 240 and and 441 with T -> C mutations, and at 261 with a T -> G mutation. Mutation choices were selected to match codons with similar codon usage.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PyrF
sequence annotation: G
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_T9150_sequence/1

PyrF
type: SequenceAnnotation
persistent identity: annotation1892252
display id: annotation1892252
version: 1
top level: PyrF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1
type: Range
persistent identity: range1892252
display id: range1892252
version: 1
direction: direction/forward
top level: PyrF
start: 1
end: 741
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1666362
display id: annotation1666362
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1
type: Range
persistent identity: range1666362
display id: range1666362
version: 1
direction: direction/forward
top level: PyrF
start: 39
end: 44
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666373
display id: annotation1666373
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1
type: Range
persistent identity: range1666373
display id: range1666373
version: 1
direction: direction/forward
top level: PyrF
start: 441
end: 446
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666364
display id: annotation1666364
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1
type: Range
persistent identity: range1666364
display id: range1666364
version: 1
direction: direction/forward
top level: PyrF
start: 261
end: 266
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666363
display id: annotation1666363
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1
type: Range
persistent identity: range1666363
display id: range1666363
version: 1
direction: direction/forward
top level: PyrF
start: 240
end: 245
orientation: inline

CmR Backwa
type: ComponentDefinition
persistent identity: BBa J31004
display id: BBa_J31004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
mutable notes: This part was cloned pSB1A2.
mutable provenance: pSB1AC3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse of CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31004_sequence/1

Reverse of CmR gene
type: SequenceAnnotation
persistent identity: annotation1884997
display id: annotation1884997
version: 1
top level: CmR Backwa
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1

https://synbiohub.org/public/igem/BBa_J31004/annotation1884997/range1884997/1
type: Range
persistent identity: range1884997
display id: range1884997
version: 1
direction: direction/forward
top level: CmR Backwa
start: 1
end: 660
orientation: inline

BBa_K863012
type: ComponentDefinition
persistent identity: BBa K863012
display id: BBa_K863012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tthl laccase (T. thermophilus) with constitutive promoter and HIS tag
mutable notes: test
mutable provenance: T. thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6 x HIS
sequence annotation: BBa_J61101
sequence annotation: J23100
sequence annotation: ATG
sequence annotation: g (original) --> a (mutation)
sequence annotation: TAA
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863012_sequence/1

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2185218
display id: annotation2185218
version: 1
top level: BBa_K863012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1
type: Range
persistent identity: range2185218
display id: range2185218
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1448
end: 1465
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2185216
display id: annotation2185216
version: 1
top level: BBa_K863012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1
type: Range
persistent identity: range2185216
display id: range2185216
version: 1
direction: direction/forward
top level: BBa_K863012
start: 44
end: 55
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2185215
display id: annotation2185215
version: 1
top level: BBa_K863012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1
type: Range
persistent identity: range2185215
display id: range2185215
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1
end: 35
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185217
display id: annotation2185217
version: 1
top level: BBa_K863012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1
type: Range
persistent identity: range2185217
display id: range2185217
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 64
orientation: inline

g (original) --> a (mutation)
type: SequenceAnnotation
persistent identity: annotation2185245
display id: annotation2185245
version: 1
top level: BBa_K863012
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1
type: Range
persistent identity: range2185245
display id: range2185245
version: 1
direction: direction/forward
top level: BBa_K863012
start: 775
end: 775
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185223
display id: annotation2185223
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1
type: Range
persistent identity: range2185223
display id: range2185223
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1466
end: 1468
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185224
display id: annotation2185224
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1
type: Range
persistent identity: range2185224
display id: range2185224
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1469
end: 1471
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2185228
display id: annotation2185228
version: 1
top level: BBa_K863012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1
type: Range
persistent identity: range2185228
display id: range2185228
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 1447
orientation: inline

BBa_M36002
type: ComponentDefinition
persistent identity: BBa M36002
display id: BBa_M36002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gluc1phos adenylyltrans (with 5'UTR & term)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details.
mutable notes: This mutation results in greater glycogen production compared to WT.
mutable provenance: The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation in the glgC gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_M36010
component: BBa_M36006
component: BBa_M36009
sequence annotation: BBa_M36006
sequence annotation: BBa_M36009
sequence annotation: BBa_M36010
sequence: https://synbiohub.org/public/igem/BBa_M36002_sequence/1

BBa_M36010
type: Component
persistent identity: component2117836
display id: component2117836
version: 1
top level: BBa_M36002
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36006
type: Component
persistent identity: component2117835
display id: component2117835
version: 1
top level: BBa_M36002
access: public
definition: BBa_M36006

BBa_M36009
type: Component
persistent identity: component2117834
display id: component2117834
version: 1
top level: BBa_M36002
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2117835
display id: annotation2117835
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117835/range2117835/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36002/annotation2117835/range2117835/1
type: Range
persistent identity: range2117835
display id: range2117835
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 48
end: 1343
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2117834
display id: annotation2117834
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117834/range2117834/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36002/annotation2117834/range2117834/1
type: Range
persistent identity: range2117834
display id: range2117834
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 1
end: 47
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2117836
display id: annotation2117836
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117836/range2117836/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36002/annotation2117836/range2117836/1
type: Range
persistent identity: range2117836
display id: range2117836
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 1344
end: 1425
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: heme oxygenase (ho1) from Synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
direction: direction/forward
top level: BBa_I15008
start: 727
end: 751
orientation: inline

CP47His
type: ComponentDefinition
persistent identity: BBa K2127004
display id: BBa_K2127004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP-47-HIS TAG
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:31:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP-47-HIS TAG
mutable notes: CP-47-HIS TAG
mutable provenance: CP-47-HIS TAG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CP47His
sequence annotation: start
sequence annotation: HisTag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2127004_sequence/1

CP47His
type: SequenceAnnotation
persistent identity: annotation2526360
display id: annotation2526360
version: 1
top level: CP47His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1
type: Range
persistent identity: range2526360
display id: range2526360
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 1548
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2526359
display id: annotation2526359
version: 1
top level: CP47His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1
type: Range
persistent identity: range2526359
display id: range2526359
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 3
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2526361
display id: annotation2526361
version: 1
top level: CP47His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1
type: Range
persistent identity: range2526361
display id: range2526361
version: 1
direction: direction/forward
top level: CP47His
start: 1522
end: 1539
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2526362
display id: annotation2526362
version: 1
top level: CP47His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1
type: Range
persistent identity: range2526362
display id: range2526362
version: 1
direction: direction/forward
top level: CP47His
start: 1543
end: 1548
orientation: inline

HyaF -> E
type: ComponentDefinition
persistent identity: BBa K1958006
display id: BBa_K1958006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaF -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:27:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: true
mutable description: HyaF is a subunit of hydrogenase 1 (Hyd-1) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structual subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaE is the sixth gene of hya operon, sharing the first few base pairs with hyaD and the last few base pairs with hyaF. HyaF is a hydrogenase maturase. Similar to hyaE, hyaF is not required for the assembly of standard O2-sensitive hydrogenases (Hyd-2 and others) and are apparently only involved in the assembly of O2-tolerant respiratory enzymes. HyaF interacts with HyaE to form a complex together with the small subunit during assembly.
mutable notes: None.
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA stop codon
sequence annotation: ATG start codon
sequence annotation: HyaF coding region
sequence: https://synbiohub.org/public/igem/BBa_K1958006_sequence/1

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2493965
display id: annotation2493965
version: 1
top level: HyaF -> E
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958006/annotation2493965/range2493965/1

https://synbiohub.org/public/igem/BBa_K1958006/annotation2493965/range2493965/1
type: Range
persistent identity: range2493965
display id: range2493965
version: 1
direction: direction/forward
top level: HyaF -> E
start: 856
end: 858
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493963
display id: annotation2493963
version: 1
top level: HyaF -> E
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958006/annotation2493963/range2493963/1

https://synbiohub.org/public/igem/BBa_K1958006/annotation2493963/range2493963/1
type: Range
persistent identity: range2493963
display id: range2493963
version: 1
direction: direction/forward
top level: HyaF -> E
start: 1
end: 3
orientation: inline

HyaF coding region
type: SequenceAnnotation
persistent identity: annotation2493964
display id: annotation2493964
version: 1
top level: HyaF -> E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958006/annotation2493964/range2493964/1

https://synbiohub.org/public/igem/BBa_K1958006/annotation2493964/range2493964/1
type: Range
persistent identity: range2493964
display id: range2493964
version: 1
direction: direction/forward
top level: HyaF -> E
start: 1
end: 858
orientation: inline

BBa_K823020
type: ComponentDefinition
persistent identity: BBa K823020
display id: BBa_K823020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (cat)
creator: Simon Kelterborn
created: 2012-08-14T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the chloramphenicol acetyltransferase (cat) gene used in Bacillus subtilis for resistance against the antibiotic chloramphenicol. We constructed it in the freiburg standard (Assembly 25) for in-frame fusion-proteins. There is a Shine-Dalgarno Sequence (RBS) optimized for Bacillus subtilis included between the XbaI and NgoIV restriction site. You can use it both N-and C terminal fusions.
mutable notes: We constructed the part in the freiburg standard (Assembly 25). We included a Bacillus optimized RBS between the XbaI and NgoIV restriction site.
mutable provenance: Plasmid: Bacillus subtilis reporter vector pAC6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823020_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179570
display id: annotation2179570
version: 1
top level: BBa_K823020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1

https://synbiohub.org/public/igem/BBa_K823020/annotation2179570/range2179570/1
type: Range
persistent identity: range2179570
display id: range2179570
version: 1
direction: direction/forward
top level: BBa_K823020
start: 1
end: 645
orientation: inline

BBa_K1072001
type: ComponentDefinition
persistent identity: BBa K1072001
display id: BBa_K1072001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH Dehydrogenase Coding Region
creator: Yichong wang, Zhiwei li, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: BBa_K1072001 is the coding region of NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ + acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Ndh coding region
sequence: https://synbiohub.org/public/igem/BBa_K1072001_sequence/1

Ndh coding region
type: SequenceAnnotation
persistent identity: annotation2334326
display id: annotation2334326
version: 1
top level: BBa_K1072001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072001/annotation2334326/range2334326/1

https://synbiohub.org/public/igem/BBa_K1072001/annotation2334326/range2334326/1
type: Range
persistent identity: range2334326
display id: range2334326
version: 1
direction: direction/forward
top level: BBa_K1072001
start: 1
end: 1305
orientation: inline

BBa_K1462050
type: ComponentDefinition
persistent identity: BBa K1462050
display id: BBa_K1462050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ccr
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462050_sequence/1

BBa_K1072003
type: ComponentDefinition
persistent identity: BBa K1072003
display id: BBa_K1072003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR+Lux pR autoinduceing luxI(lva tag)+Lux pR controlled Ndh
creator: Yichong wang, Zhiwei li, Xihao liao and Junjie zhang
created: 2013-09-05T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072003 is a composite device, including LuxI，LuxR and Ndh genes. LuxR， controlled by Lux pI, generate LuxR protein, which will combined with AHL and activate Lux pR promotor. that complex increases the transcriptional rate of the lux pR promotor while also decrease the transceiptional rate of the Lux pL promoter. LuxI is the generator of AHL controlled by Lux pR promoter. Ndh was activated by AHL and LuxR complex and yields H2O2;
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072001
component: BBa_B0034
component: BBa_R0062
component: BBa_B0010
component: BBa_B0012
component: BBa_C0061
component: BBa_R0063
component: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_K1072001
sequence annotation: BBa_R0063
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K1072003_sequence/1

BBa_K1072001
type: Component
persistent identity: component2335201
display id: component2335201
version: 1
top level: BBa_K1072003
access: public
definition: BBa_K1072001

BBa_B0034
type: Component
persistent identity: component2335182
display id: component2335182
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2335194
display id: component2335194
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component2335202
display id: component2335202
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2335199
display id: component2335199
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2335172
display id: component2335172
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component2335204
display id: component2335204
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2335187
display id: component2335187
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2335163
display id: component2335163
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: Component
persistent identity: component2335184
display id: component2335184
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_R0062
type: Component
persistent identity: component2335177
display id: component2335177
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2335189
display id: component2335189
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0063
type: Component
persistent identity: component2335157
display id: component2335157
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component2335166
display id: component2335166
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component2335170
display id: component2335170
version: 1
top level: BBa_K1072003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335199
display id: annotation2335199
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335199/range2335199/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335199/range2335199/1
type: Range
persistent identity: range2335199
display id: range2335199
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 2036
end: 2047
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2335194
display id: annotation2335194
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335194/range2335194/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335194/range2335194/1
type: Range
persistent identity: range2335194
display id: range2335194
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1973
end: 2027
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2335177
display id: annotation2335177
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335177/range2335177/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335177/range2335177/1
type: Range
persistent identity: range2335177
display id: range2335177
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1104
end: 1158
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335187
display id: annotation2335187
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335187/range2335187/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335187/range2335187/1
type: Range
persistent identity: range2335187
display id: range2335187
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1836
end: 1915
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335189
display id: annotation2335189
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335189/range2335189/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335189/range2335189/1
type: Range
persistent identity: range2335189
display id: range2335189
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1924
end: 1964
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2335184
display id: annotation2335184
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335184/range2335184/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335184/range2335184/1
type: Range
persistent identity: range2335184
display id: range2335184
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1185
end: 1802
orientation: inline

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2335201
display id: annotation2335201
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335201/range2335201/1
component: BBa_K1072001

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335201/range2335201/1
type: Range
persistent identity: range2335201
display id: range2335201
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 2056
end: 3360
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2335157
display id: annotation2335157
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335157/range2335157/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335157/range2335157/1
type: Range
persistent identity: range2335157
display id: range2335157
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1
end: 151
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2335166
display id: annotation2335166
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335166/range2335166/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335166/range2335166/1
type: Range
persistent identity: range2335166
display id: range2335166
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 178
end: 933
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335172
display id: annotation2335172
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335172/range2335172/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335172/range2335172/1
type: Range
persistent identity: range2335172
display id: range2335172
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 1055
end: 1095
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335163
display id: annotation2335163
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335163/range2335163/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335163/range2335163/1
type: Range
persistent identity: range2335163
display id: range2335163
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 160
end: 171
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2335182
display id: annotation2335182
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335182/range2335182/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335182/range2335182/1
type: Range
persistent identity: range2335182
display id: range2335182
version: 1
direction: direction/reverse
top level: BBa_K1072003
start: 1167
end: 1178
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335170
display id: annotation2335170
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335170/range2335170/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335170/range2335170/1
type: Range
persistent identity: range2335170
display id: range2335170
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 967
end: 1046
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2335202
display id: annotation2335202
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335202/range2335202/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335202/range2335202/1
type: Range
persistent identity: range2335202
display id: range2335202
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 3369
end: 3448
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2335204
display id: annotation2335204
version: 1
top level: BBa_K1072003
location: https://synbiohub.org/public/igem/BBa_K1072003/annotation2335204/range2335204/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072003/annotation2335204/range2335204/1
type: Range
persistent identity: range2335204
display id: range2335204
version: 1
direction: direction/forward
top level: BBa_K1072003
start: 3457
end: 3497
orientation: inline

BBa_K147002
type: ComponentDefinition
persistent identity: BBa K147002
display id: BBa_K147002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylE
creator: Nathan Puhl
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: true
group u list: _129_242_
m user id: 0
owner id: 1757
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Blah Blah Blah
mutable notes: Blah Blah Blah
mutable provenance: Blah Blah Blah
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K147002_sequence/1

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_K1433008
sequence annotation: BBa_R0082
sequence annotation: BBa_K1433008
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_K1554002
type: ComponentDefinition
persistent identity: BBa K1554002
display id: BBa_K1554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HarFAR
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fatty alcohol reductase
mutable notes: BioBrick compatible
mutable provenance: It comes from Helicoverpa armigera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554002_sequence/1

BBa_K567010
type: ComponentDefinition
persistent identity: BBa K567010
display id: BBa_K567010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-8AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 8 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNAArg (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 8 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28a. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ATG
sequence annotation: luciferase-8AGG
sequence annotation: 8AGG
sequence annotation: PT7
sequence annotation: rbs
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K567010_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2150775
display id: annotation2150775
version: 1
top level: BBa_K567010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150775/range2150775/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150775/range2150775/1
type: Range
persistent identity: range2150775
display id: range2150775
version: 1
direction: direction/forward
top level: BBa_K567010
start: 89
end: 91
orientation: inline

luciferase-8AGG
type: SequenceAnnotation
persistent identity: annotation2150777
display id: annotation2150777
version: 1
top level: BBa_K567010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150777/range2150777/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150777/range2150777/1
type: Range
persistent identity: range2150777
display id: range2150777
version: 1
direction: direction/forward
top level: BBa_K567010
start: 89
end: 1774
orientation: inline

8AGG
type: SequenceAnnotation
persistent identity: annotation2150776
display id: annotation2150776
version: 1
top level: BBa_K567010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150776/range2150776/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150776/range2150776/1
type: Range
persistent identity: range2150776
display id: range2150776
version: 1
direction: direction/forward
top level: BBa_K567010
start: 95
end: 118
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2150805
display id: annotation2150805
version: 1
top level: BBa_K567010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150805/range2150805/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150805/range2150805/1
type: Range
persistent identity: range2150805
display id: range2150805
version: 1
direction: direction/forward
top level: BBa_K567010
start: 2
end: 18
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150808
display id: annotation2150808
version: 1
top level: BBa_K567010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150808/range2150808/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150808/range2150808/1
type: Range
persistent identity: range2150808
display id: range2150808
version: 1
direction: direction/forward
top level: BBa_K567010
start: 75
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150806
display id: annotation2150806
version: 1
top level: BBa_K567010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567010/annotation2150806/range2150806/1

https://synbiohub.org/public/igem/BBa_K567010/annotation2150806/range2150806/1
type: Range
persistent identity: range2150806
display id: range2150806
version: 1
direction: direction/forward
top level: BBa_K567010
start: 21
end: 44
orientation: inline

BBa_M36007
type: ComponentDefinition
persistent identity: BBa M36007
display id: BBa_M36007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glycogen synthase
creator: Jesse Palmer, Elliot Lui
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details.
mutable provenance: Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_M36007_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2119213
display id: annotation2119213
version: 1
top level: BBa_M36007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36007/annotation2119213/range2119213/1

https://synbiohub.org/public/igem/BBa_M36007/annotation2119213/range2119213/1
type: Range
persistent identity: range2119213
display id: range2119213
version: 1
direction: direction/forward
top level: BBa_M36007
start: 1432
end: 1434
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2119212
display id: annotation2119212
version: 1
top level: BBa_M36007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36007/annotation2119212/range2119212/1

https://synbiohub.org/public/igem/BBa_M36007/annotation2119212/range2119212/1
type: Range
persistent identity: range2119212
display id: range2119212
version: 1
direction: direction/forward
top level: BBa_M36007
start: 1
end: 3
orientation: inline

HyaA -> E.
type: ComponentDefinition
persistent identity: BBa K1958001
display id: BBa_K1958001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaA -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T08:59:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HyaA is the small subunit of hydrogenase 1 (Hyd-1, from MBH family) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structural subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaA is the first gene of hya operon, sharing the last few base pairs with hyaB. Under carbon starvation upon entering stationary phase, expression of the hya operon, which encodes Hyd-1, is regulated by sigma-38. The small subunit exhibits sequence homologies to subunits of NADH: ubiquinone oxidoreductase (Complex I). This small subunit of this [Ni-Fe] hydrogenase contains 4 Fe-S clusters ([Fe3S4], [Fe4S4]d, [Fe4S3]) that play a role in ET to and from the large subunit for H2 reduction/oxidation. The supernumerary cysteine in the proximal cluster of the small subunit also plays a role in O2 tolerance.
mutable notes: None
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop codon
sequence annotation: ATG start codon
sequence annotation: HyaA coding region
sequence: https://synbiohub.org/public/igem/BBa_K1958001_sequence/1

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2493845
display id: annotation2493845
version: 1
top level: HyaA -> E.
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958001/annotation2493845/range2493845/1

https://synbiohub.org/public/igem/BBa_K1958001/annotation2493845/range2493845/1
type: Range
persistent identity: range2493845
display id: range2493845
version: 1
direction: direction/forward
top level: HyaA -> E.
start: 1117
end: 1119
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493843
display id: annotation2493843
version: 1
top level: HyaA -> E.
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958001/annotation2493843/range2493843/1

https://synbiohub.org/public/igem/BBa_K1958001/annotation2493843/range2493843/1
type: Range
persistent identity: range2493843
display id: range2493843
version: 1
direction: direction/forward
top level: HyaA -> E.
start: 1
end: 3
orientation: inline

HyaA coding region
type: SequenceAnnotation
persistent identity: annotation2493846
display id: annotation2493846
version: 1
top level: HyaA -> E.
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958001/annotation2493846/range2493846/1

https://synbiohub.org/public/igem/BBa_K1958001/annotation2493846/range2493846/1
type: Range
persistent identity: range2493846
display id: range2493846
version: 1
direction: direction/forward
top level: HyaA -> E.
start: 1
end: 1119
orientation: inline

BBa_K1031923
type: ComponentDefinition
persistent identity: BBa K1031923
display id: BBa_K1031923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23109-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylB
sequence annotation: J23109
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031923_sequence/1

XylB
type: SequenceAnnotation
persistent identity: annotation2370040
display id: annotation2370040
version: 1
top level: BBa_K1031923
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1
type: Range
persistent identity: range2370040
display id: range2370040
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 63
end: 1169
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2370039
display id: annotation2370039
version: 1
top level: BBa_K1031923
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1
type: Range
persistent identity: range2370039
display id: range2370039
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370041
display id: annotation2370041
version: 1
top level: BBa_K1031923
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1
type: Range
persistent identity: range2370041
display id: range2370041
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1176
end: 1304
orientation: inline

BBa_K863006
type: ComponentDefinition
persistent identity: BBa K863006
display id: BBa_K863006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli laccase ORF
mutable notes: test
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863006_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2184435
display id: annotation2184435
version: 1
top level: BBa_K863006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1

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type: Range
persistent identity: range2184435
display id: range2184435
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184436
display id: annotation2184436
version: 1
top level: BBa_K863006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1
type: Range
persistent identity: range2184436
display id: range2184436
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1549
end: 1551
orientation: inline

BBa_K777124
type: ComponentDefinition
persistent identity: BBa K777124
display id: BBa_K777124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yhjH under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants. Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
mutable notes: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3' ** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
mutable provenance: * ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777121
sequence annotation: BBa_K777121
sequence annotation: BBa_J23114
sequence: https://synbiohub.org/public/igem/BBa_K777124_sequence/1

BBa_J23114
type: Component
persistent identity: component2193022
display id: component2193022
version: 1
top level: BBa_K777124
access: public
definition: https://synbiohub.org/public/igem/BBa_J23114/1

BBa_K777121
type: Component
persistent identity: component2193024
display id: component2193024
version: 1
top level: BBa_K777124
access: public
definition: BBa_K777121

BBa_K777121
type: SequenceAnnotation
persistent identity: annotation2193024
display id: annotation2193024
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
component: BBa_K777121

https://synbiohub.org/public/igem/BBa_K777124/annotation2193024/range2193024/1
type: Range
persistent identity: range2193024
display id: range2193024
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 42
end: 809
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2193022
display id: annotation2193022
version: 1
top level: BBa_K777124
location: https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777124/annotation2193022/range2193022/1
type: Range
persistent identity: range2193022
display id: range2193022
version: 1
direction: direction/reverse
top level: BBa_K777124
start: 1
end: 35
orientation: inline

BBa_M36284
type: ComponentDefinition
persistent identity: BBa M36284
display id: BBa_M36284
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Human Alcohol Dehydrogenase 1B actuator
creator: Sunil Bodapati, Julia Joung
created: 2011-11-29T12:00:00Z
modified: 2015-05-08T01:14:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11068
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our proposed gene sequence is an actuator that produces ADH1B following an RNA polymerase per second (PoPS) signal from a sensor. The sensor will be followed by a bi-cistronic ribosome binding sequence provided by BIOFAB (BD2). This type of ribosome binding sequence contains a ribosome binding site followed by the DNA sequence TAATG, which terminates translation of the first part with stop codon TAA. A second ribosome can bind in the sequence and start translation of the gene with start codon ATG. The bi-cistronic ribosome binding sequence prevents the protein???s ribosome binding site from forming a hairpin loop with another part of the sequence, which would reduce the amount of translation. Specifically, our group chose to use BD2 because it offers consistently high to medium expression of the gene of interest. After BD2, we have inserted the gene sequence of ADH1B, which we constructed using the protein sequence from protein database UniProt that was cross referenced with NCBI. Since we are inserting a eukaryotic gene into a prokaryote, we optimized the DNA sequence for E. Coli using a codon optimizing chart provided by Sharp et al. (1988), selecting for highly transcribed genes as our objective is to produce large amounts of the enzyme. Our ADH1B gene was followed by a histidine tag consisting of 6 histidines that can be used to purify the enzyme with nickel affinity chromatography. Then we put a stop codon TAG that terminates translation. Lastly, we placed a transcription terminator, part apFAB391 provided by Biofab, that terminates transcription 99% of the time because our objective is to transcribe ADH1B so we would want to prevent transcribing other proteins on the same plasmid to conserve energy.
mutable notes: We maximized expression by using a highly expressing bicistronic RBS. We needed a way to purify the enzyme post production. The histidine tag allows us to perform nickel affinity chromatography to isolate purified protein.
mutable provenance: Bicistonic RBS - BioFab: apFAB563 ADH1B - partsregistry: BBa_M36666 Histidine Tag - 6 histidines Stop Codon - generic TAG Terminator - BIOFAB: apFAB391
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_M36282
component: BBa_M36281
component: BBa_M36280
sequence annotation: BBa_M36281
sequence annotation: BBa_M36282
sequence annotation: BBa_M36280
sequence: https://synbiohub.org/public/igem/BBa_M36284_sequence/1

BBa_M36282
type: Component
persistent identity: component2166161
display id: component2166161
version: 1
top level: BBa_M36284
access: public
definition: https://synbiohub.org/public/igem/BBa_M36282/1

BBa_M36281
type: Component
persistent identity: component2166165
display id: component2166165
version: 1
top level: BBa_M36284
access: public
definition: https://synbiohub.org/public/igem/BBa_M36281/1

BBa_M36280
type: Component
persistent identity: component2166164
display id: component2166164
version: 1
top level: BBa_M36284
access: public
definition: https://synbiohub.org/public/igem/BBa_M36280/1

BBa_M36281
type: SequenceAnnotation
persistent identity: annotation2166165
display id: annotation2166165
version: 1
top level: BBa_M36284
location: https://synbiohub.org/public/igem/BBa_M36284/annotation2166165/range2166165/1
component: BBa_M36281

https://synbiohub.org/public/igem/BBa_M36284/annotation2166165/range2166165/1
type: Range
persistent identity: range2166165
display id: range2166165
version: 1
direction: direction/reverse
top level: BBa_M36284
start: 1248
end: 1329
orientation: inline

BBa_M36282
type: SequenceAnnotation
persistent identity: annotation2166161
display id: annotation2166161
version: 1
top level: BBa_M36284
location: https://synbiohub.org/public/igem/BBa_M36284/annotation2166161/range2166161/1
component: BBa_M36282

https://synbiohub.org/public/igem/BBa_M36284/annotation2166161/range2166161/1
type: Range
persistent identity: range2166161
display id: range2166161
version: 1
direction: direction/reverse
top level: BBa_M36284
start: 1
end: 88
orientation: inline

BBa_M36280
type: SequenceAnnotation
persistent identity: annotation2166164
display id: annotation2166164
version: 1
top level: BBa_M36284
location: https://synbiohub.org/public/igem/BBa_M36284/annotation2166164/range2166164/1
component: BBa_M36280

https://synbiohub.org/public/igem/BBa_M36284/annotation2166164/range2166164/1
type: Range
persistent identity: range2166164
display id: range2166164
version: 1
direction: direction/reverse
top level: BBa_M36284
start: 97
end: 1239
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aad9 Spectinomycin Resistance Gene
creator: Chris Hirst
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
direction: direction/forward
top level: Aad9
start: 769
end: 771
orientation: inline

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
direction: direction/forward
top level: Aad9
start: 1
end: 765
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
direction: direction/forward
top level: Aad9
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
direction: direction/forward
top level: Aad9
start: 766
end: 768
orientation: inline

BBa_K1172203
type: ComponentDefinition
persistent identity: BBa K1172203
display id: BBa_K1172203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli with P T7 and RBS
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K525998
component: BBa_K1172201
sequence annotation: BBa_K1172201
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1172203_sequence/1

BBa_K525998
type: Component
persistent identity: component2332929
display id: component2332929
version: 1
top level: BBa_K1172203
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1172201
type: Component
persistent identity: component2332931
display id: component2332931
version: 1
top level: BBa_K1172203
access: public
definition: gldA

BBa_K1172201
type: SequenceAnnotation
persistent identity: annotation2332931
display id: annotation2332931
version: 1
top level: BBa_K1172203
location: https://synbiohub.org/public/igem/BBa_K1172203/annotation2332931/range2332931/1
component: BBa_K1172201

https://synbiohub.org/public/igem/BBa_K1172203/annotation2332931/range2332931/1
type: Range
persistent identity: range2332931
display id: range2332931
version: 1
direction: direction/reverse
top level: BBa_K1172203
start: 39
end: 1142
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2332929
display id: annotation2332929
version: 1
top level: BBa_K1172203
location: https://synbiohub.org/public/igem/BBa_K1172203/annotation2332929/range2332929/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1172203/annotation2332929/range2332929/1
type: Range
persistent identity: range2332929
display id: range2332929
version: 1
direction: direction/reverse
top level: BBa_K1172203
start: 1
end: 32
orientation: inline

BBa_K633014
type: ComponentDefinition
persistent identity: BBa K633014
display id: BBa_K633014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-06-19T01:50:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator
mutable notes: asdasdadd
mutable provenance: asdasda
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13458
component: BBa_K633002
component: BBa_K206000
component: BBa_K633001
sequence annotation: BBa_K633002
sequence annotation: BBa_K206000
sequence annotation: BBa_K633001
sequence annotation: BBa_I13458
sequence: https://synbiohub.org/public/igem/BBa_K633014_sequence/1

BBa_I13458
type: Component
persistent identity: component2159631
display id: component2159631
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_K633002
type: Component
persistent identity: component2159639
display id: component2159639
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K633002/1

BBa_K206000
type: Component
persistent identity: component2159635
display id: component2159635
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K633001
type: Component
persistent identity: component2159642
display id: component2159642
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K633001/1

BBa_K633002
type: SequenceAnnotation
persistent identity: annotation2159639
display id: annotation2159639
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
component: BBa_K633002

https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
type: Range
persistent identity: range2159639
display id: range2159639
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1261
end: 3173
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2159635
display id: annotation2159635
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
type: Range
persistent identity: range2159635
display id: range2159635
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1123
end: 1252
orientation: inline

BBa_K633001
type: SequenceAnnotation
persistent identity: annotation2159642
display id: annotation2159642
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
component: BBa_K633001

https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
type: Range
persistent identity: range2159642
display id: range2159642
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 3182
end: 4057
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2159631
display id: annotation2159631
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
type: Range
persistent identity: range2159631
display id: range2159631
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1
end: 1114
orientation: inline

BBa_K1819001
type: ComponentDefinition
persistent identity: BBa K1819001
display id: BBa_K1819001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Latex Clearing Protein (Lcp) - rubber degradation
creator: Lais Ribovski
created: 2015-09-12T11:00:00Z
modified: 2015-09-13T08:46:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2244_
m user id: 21190
owner id: 21190
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.
mutable notes: Because Lcp is secreted by Streptomyces sp, it contains a 5???-signal peptide that was identified using signal peptide predictor server (4) (http://www.compgen.org/tools/PRED-TAT). We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites.
mutable provenance: Lcp from actinomycete Streptomyces sp. strain K30
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Lcp
sequence annotation: SacI site
sequence annotation: NdeI site
sequence: https://synbiohub.org/public/igem/BBa_K1819001_sequence/1

Lcp
type: SequenceAnnotation
persistent identity: annotation2453650
display id: annotation2453650
version: 1
top level: BBa_K1819001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1819001/annotation2453650/range2453650/1

https://synbiohub.org/public/igem/BBa_K1819001/annotation2453650/range2453650/1
type: Range
persistent identity: range2453650
display id: range2453650
version: 1
direction: direction/forward
top level: BBa_K1819001
start: 7
end: 1132
orientation: inline

SacI site
type: SequenceAnnotation
persistent identity: annotation2453669
display id: annotation2453669
version: 1
top level: BBa_K1819001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1819001/annotation2453669/range2453669/1

https://synbiohub.org/public/igem/BBa_K1819001/annotation2453669/range2453669/1
type: Range
persistent identity: range2453669
display id: range2453669
version: 1
direction: direction/forward
top level: BBa_K1819001
start: 1
end: 6
orientation: inline

NdeI site
type: SequenceAnnotation
persistent identity: annotation2453670
display id: annotation2453670
version: 1
top level: BBa_K1819001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1819001/annotation2453670/range2453670/1

https://synbiohub.org/public/igem/BBa_K1819001/annotation2453670/range2453670/1
type: Range
persistent identity: range2453670
display id: range2453670
version: 1
direction: direction/forward
top level: BBa_K1819001
start: 1133
end: 1138
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

BBa_K258006
type: ComponentDefinition
persistent identity: BBa K258006
display id: BBa_K258006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
created: 2009-10-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi.
mutable notes: The sequence was optimized for E.coli with Biobrick restriction sites
mutable provenance: The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258006_sequence/1

BBa_K863000
type: ComponentDefinition
persistent identity: BBa K863000
display id: BBa_K863000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7- Promotor BBa_I719005
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: 6 x HIS
sequence annotation: TAA
sequence annotation: A (Original) T (Mutation)
sequence annotation: bpul
sequence: https://synbiohub.org/public/igem/BBa_K863000_sequence/1

T7- Promotor BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2184908
display id: annotation2184908
version: 1
top level: BBa_K863000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1
type: Range
persistent identity: range2184908
display id: range2184908
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1
end: 23
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184909
display id: annotation2184909
version: 1
top level: BBa_K863000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1
type: Range
persistent identity: range2184909
display id: range2184909
version: 1
direction: direction/forward
top level: BBa_K863000
start: 25
end: 30
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184910
display id: annotation2184910
version: 1
top level: BBa_K863000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1
type: Range
persistent identity: range2184910
display id: range2184910
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 36
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2184913
display id: annotation2184913
version: 1
top level: BBa_K863000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1
type: Range
persistent identity: range2184913
display id: range2184913
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1557
end: 1581
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184914
display id: annotation2184914
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1
type: Range
persistent identity: range2184914
display id: range2184914
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1582
end: 1584
orientation: inline

A (Original) T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184912
display id: annotation2184912
version: 1
top level: BBa_K863000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1
type: Range
persistent identity: range2184912
display id: range2184912
version: 1
direction: direction/forward
top level: BBa_K863000
start: 834
end: 834
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184915
display id: annotation2184915
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1
type: Range
persistent identity: range2184915
display id: range2184915
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1585
end: 1587
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184911
display id: annotation2184911
version: 1
top level: BBa_K863000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1
type: Range
persistent identity: range2184911
display id: range2184911
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 1556
orientation: inline

BBa_K1331006
type: ComponentDefinition
persistent identity: BBa K1331006
display id: BBa_K1331006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1331004
component: BBa_K1331001
sequence annotation: BBa_K1331001
sequence annotation: BBa_K1331004
sequence: https://synbiohub.org/public/igem/BBa_K1331006_sequence/1

BBa_K1331004
type: Component
persistent identity: component2386717
display id: component2386717
version: 1
top level: BBa_K1331006
access: public
definition: BBa_K1331004

BBa_K1331001
type: Component
persistent identity: component2386715
display id: component2386715
version: 1
top level: BBa_K1331006
access: public
definition: BBa_K1331001

BBa_K1331001
type: SequenceAnnotation
persistent identity: annotation2386715
display id: annotation2386715
version: 1
top level: BBa_K1331006
location: https://synbiohub.org/public/igem/BBa_K1331006/annotation2386715/range2386715/1
component: BBa_K1331001

https://synbiohub.org/public/igem/BBa_K1331006/annotation2386715/range2386715/1
type: Range
persistent identity: range2386715
display id: range2386715
version: 1
direction: direction/reverse
top level: BBa_K1331006
start: 1
end: 947
orientation: inline

BBa_K1331004
type: SequenceAnnotation
persistent identity: annotation2386717
display id: annotation2386717
version: 1
top level: BBa_K1331006
location: https://synbiohub.org/public/igem/BBa_K1331006/annotation2386717/range2386717/1
component: BBa_K1331004

https://synbiohub.org/public/igem/BBa_K1331006/annotation2386717/range2386717/1
type: Range
persistent identity: range2386717
display id: range2386717
version: 1
direction: direction/reverse
top level: BBa_K1331006
start: 956
end: 2262
orientation: inline

BBa_K103016
type: ComponentDefinition
persistent identity: BBa K103016
display id: BBa_K103016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA_linker_omega_linker
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
component: BBa_K103012
component: BBa_K103006
sequence annotation: BBa_K103012
sequence annotation: BBa_K103006
sequence: https://synbiohub.org/public/igem/BBa_K103016_sequence/1

BBa_K103012
type: Component
persistent identity: component1993524
display id: component1993524
version: 1
top level: BBa_K103016
access: public
definition: https://synbiohub.org/public/igem/BBa_K103012/1

BBa_K103006
type: Component
persistent identity: component1993520
display id: component1993520
version: 1
top level: BBa_K103016
access: public
definition: https://synbiohub.org/public/igem/BBa_K103006/1

BBa_K103012
type: SequenceAnnotation
persistent identity: annotation1993524
display id: annotation1993524
version: 1
top level: BBa_K103016
location: https://synbiohub.org/public/igem/BBa_K103016/annotation1993524/range1993524/1
component: BBa_K103012

https://synbiohub.org/public/igem/BBa_K103016/annotation1993524/range1993524/1
type: Range
persistent identity: range1993524
display id: range1993524
version: 1
direction: direction/reverse
top level: BBa_K103016
start: 465
end: 809
orientation: inline

BBa_K103006
type: SequenceAnnotation
persistent identity: annotation1993520
display id: annotation1993520
version: 1
top level: BBa_K103016
location: https://synbiohub.org/public/igem/BBa_K103016/annotation1993520/range1993520/1
component: BBa_K103006

https://synbiohub.org/public/igem/BBa_K103016/annotation1993520/range1993520/1
type: Range
persistent identity: range1993520
display id: range1993520
version: 1
direction: direction/reverse
top level: BBa_K103016
start: 1
end: 464
orientation: inline

BBa_K748003
type: ComponentDefinition
persistent identity: BBa K748003
display id: BBa_K748003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yddV is a Diguanylate Cyclase-Genomic.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748003_sequence/1

BBa_K1587004
type: ComponentDefinition
persistent identity: BBa K1587004
display id: BBa_K1587004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Butyrate synthesis pathway regulated by constitutive promoter p(Bla)
creator: Alexandre Le Scornet
created: 2015-09-13T11:00:00Z
modified: 2015-09-16T11:58:25Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2004_
m user id: 24598
owner id: 24598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfotunatly NMR analysis did not yield a significant over production of butyrate in E. coli.
mutable notes: p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site
mutable provenance: tesB, ccr, crt, and hbd sequence are from Clostridium acetobutylicum. atoB sequence is from E.coli. The strong RBS and Terminator were used according to respectively BBa_B0030 and BBa_B1006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rbs
sequence annotation: ccr
sequence annotation: atoB
sequence annotation: tesB
sequence annotation: Terminator
sequence annotation: hbd
sequence annotation: p(Bla)
sequence annotation: crt
sequence: https://synbiohub.org/public/igem/BBa_K1587004_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2461502
display id: annotation2461502
version: 1
top level: BBa_K1587004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461502/range2461502/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461502/range2461502/1
type: Range
persistent identity: range2461502
display id: range2461502
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 3954
end: 3968
orientation: inline

ccr
type: SequenceAnnotation
persistent identity: annotation2461495
display id: annotation2461495
version: 1
top level: BBa_K1587004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461495/range2461495/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461495/range2461495/1
type: Range
persistent identity: range2461495
display id: range2461495
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 57
end: 1406
orientation: inline

atoB
type: SequenceAnnotation
persistent identity: annotation2461503
display id: annotation2461503
version: 1
top level: BBa_K1587004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461503/range2461503/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461503/range2461503/1
type: Range
persistent identity: range2461503
display id: range2461503
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 3969
end: 5153
orientation: inline

tesB
type: SequenceAnnotation
persistent identity: annotation2461501
display id: annotation2461501
version: 1
top level: BBa_K1587004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461501/range2461501/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461501/range2461501/1
type: Range
persistent identity: range2461501
display id: range2461501
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 3093
end: 3953
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2461504
display id: annotation2461504
version: 1
top level: BBa_K1587004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461504/range2461504/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461504/range2461504/1
type: Range
persistent identity: range2461504
display id: range2461504
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 5154
end: 5192
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2461496
display id: annotation2461496
version: 1
top level: BBa_K1587004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461496/range2461496/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461496/range2461496/1
type: Range
persistent identity: range2461496
display id: range2461496
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 1413
end: 1427
orientation: inline

hbd
type: SequenceAnnotation
persistent identity: annotation2461497
display id: annotation2461497
version: 1
top level: BBa_K1587004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461497/range2461497/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461497/range2461497/1
type: Range
persistent identity: range2461497
display id: range2461497
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 1428
end: 2276
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2461498
display id: annotation2461498
version: 1
top level: BBa_K1587004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461498/range2461498/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461498/range2461498/1
type: Range
persistent identity: range2461498
display id: range2461498
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 2277
end: 2291
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2461500
display id: annotation2461500
version: 1
top level: BBa_K1587004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461500/range2461500/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461500/range2461500/1
type: Range
persistent identity: range2461500
display id: range2461500
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 3078
end: 3092
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2461494
display id: annotation2461494
version: 1
top level: BBa_K1587004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461494/range2461494/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461494/range2461494/1
type: Range
persistent identity: range2461494
display id: range2461494
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 42
end: 56
orientation: inline

p(Bla)
type: SequenceAnnotation
persistent identity: annotation2461493
display id: annotation2461493
version: 1
top level: BBa_K1587004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461493/range2461493/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461493/range2461493/1
type: Range
persistent identity: range2461493
display id: range2461493
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 1
end: 35
orientation: inline

crt
type: SequenceAnnotation
persistent identity: annotation2461499
display id: annotation2461499
version: 1
top level: BBa_K1587004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587004/annotation2461499/range2461499/1

https://synbiohub.org/public/igem/BBa_K1587004/annotation2461499/range2461499/1
type: Range
persistent identity: range2461499
display id: range2461499
version: 1
direction: direction/forward
top level: BBa_K1587004
start: 2292
end: 3077
orientation: inline

BBa_K567021
type: ComponentDefinition
persistent identity: BBa K567021
display id: BBa_K567021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-2x4AGG(111bp)
creator: You Wang
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8478
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: fdfdf
mutable notes: fdfdf
mutable provenance: fdfdfd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: 111bp interval
sequence annotation: 4AGG
sequence annotation: PT7
sequence annotation: rbs
sequence annotation: lac operator
sequence annotation: ATG
sequence annotation: luciferase
sequence: https://synbiohub.org/public/igem/BBa_K567021_sequence/1

111bp interval
type: SequenceAnnotation
persistent identity: annotation2161601
display id: annotation2161601
version: 1
top level: BBa_K567021
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2161601/range2161601/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2161601/range2161601/1
type: Range
persistent identity: range2161601
display id: range2161601
version: 1
direction: direction/forward
top level: BBa_K567021
start: 107
end: 217
orientation: inline

4AGG
type: SequenceAnnotation
persistent identity: annotation2155473
display id: annotation2155473
version: 1
top level: BBa_K567021
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155473/range2155473/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155473/range2155473/1
type: Range
persistent identity: range2155473
display id: range2155473
version: 1
direction: direction/forward
top level: BBa_K567021
start: 95
end: 106
orientation: inline

PT7
type: SequenceAnnotation
persistent identity: annotation2155470
display id: annotation2155470
version: 1
top level: BBa_K567021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155470/range2155470/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155470/range2155470/1
type: Range
persistent identity: range2155470
display id: range2155470
version: 1
direction: direction/forward
top level: BBa_K567021
start: 2
end: 18
orientation: inline

4AGG
type: SequenceAnnotation
persistent identity: annotation2155474
display id: annotation2155474
version: 1
top level: BBa_K567021
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155474/range2155474/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155474/range2155474/1
type: Range
persistent identity: range2155474
display id: range2155474
version: 1
direction: direction/forward
top level: BBa_K567021
start: 218
end: 229
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2155472
display id: annotation2155472
version: 1
top level: BBa_K567021
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155472/range2155472/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155472/range2155472/1
type: Range
persistent identity: range2155472
display id: range2155472
version: 1
direction: direction/forward
top level: BBa_K567021
start: 75
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2155471
display id: annotation2155471
version: 1
top level: BBa_K567021
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155471/range2155471/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155471/range2155471/1
type: Range
persistent identity: range2155471
display id: range2155471
version: 1
direction: direction/forward
top level: BBa_K567021
start: 21
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2155469
display id: annotation2155469
version: 1
top level: BBa_K567021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155469/range2155469/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155469/range2155469/1
type: Range
persistent identity: range2155469
display id: range2155469
version: 1
direction: direction/forward
top level: BBa_K567021
start: 89
end: 91
orientation: inline

luciferase
type: SequenceAnnotation
persistent identity: annotation2155468
display id: annotation2155468
version: 1
top level: BBa_K567021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567021/annotation2155468/range2155468/1

https://synbiohub.org/public/igem/BBa_K567021/annotation2155468/range2155468/1
type: Range
persistent identity: range2155468
display id: range2155468
version: 1
direction: direction/forward
top level: BBa_K567021
start: 89
end: 1885
orientation: inline

BBa_K1598001
type: ComponentDefinition
persistent identity: BBa K1598001
display id: BBa_K1598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPH1 (Tryptophan hydroxylase 1)
creator: Marta Napiorkowska
created: 2015-06-17T11:00:00Z
modified: 2015-08-27T02:25:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27180
owner id: 27256
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Converts the amino acid tryptophan into 5-HTP, which is a precursor to serotonin. Soon to be edit
mutable notes: Optimised for E.coli, removed illegal restriction enzyme sites
mutable provenance: Human (Homo sapiens)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1598001_sequence/1

BBa_K567009
type: ComponentDefinition
persistent identity: BBa K567009
display id: BBa_K567009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PT7-Luc-4AGG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 4 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg) (BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: PCR is used to insert the 4 AGG codons into the gene after ATG.
mutable provenance: T7 promoter is derived from pET28a. Wild type Luciferase from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PT7
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: 4AGG
sequence annotation: rbs
sequence annotation: luciferase
sequence: https://synbiohub.org/public/igem/BBa_K567009_sequence/1

PT7
type: SequenceAnnotation
persistent identity: annotation2150788
display id: annotation2150788
version: 1
top level: BBa_K567009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150788/range2150788/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150788/range2150788/1
type: Range
persistent identity: range2150788
display id: range2150788
version: 1
direction: direction/forward
top level: BBa_K567009
start: 2
end: 18
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150772
display id: annotation2150772
version: 1
top level: BBa_K567009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150772/range2150772/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150772/range2150772/1
type: Range
persistent identity: range2150772
display id: range2150772
version: 1
direction: direction/forward
top level: BBa_K567009
start: 89
end: 91
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150789
display id: annotation2150789
version: 1
top level: BBa_K567009
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150789/range2150789/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150789/range2150789/1
type: Range
persistent identity: range2150789
display id: range2150789
version: 1
direction: direction/forward
top level: BBa_K567009
start: 21
end: 44
orientation: inline

4AGG
type: SequenceAnnotation
persistent identity: annotation2150773
display id: annotation2150773
version: 1
top level: BBa_K567009
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150773/range2150773/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150773/range2150773/1
type: Range
persistent identity: range2150773
display id: range2150773
version: 1
direction: direction/forward
top level: BBa_K567009
start: 95
end: 106
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150790
display id: annotation2150790
version: 1
top level: BBa_K567009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150790/range2150790/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150790/range2150790/1
type: Range
persistent identity: range2150790
display id: range2150790
version: 1
direction: direction/forward
top level: BBa_K567009
start: 75
end: 81
orientation: inline

luciferase
type: SequenceAnnotation
persistent identity: annotation2150774
display id: annotation2150774
version: 1
top level: BBa_K567009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567009/annotation2150774/range2150774/1

https://synbiohub.org/public/igem/BBa_K567009/annotation2150774/range2150774/1
type: Range
persistent identity: range2150774
display id: range2150774
version: 1
direction: direction/forward
top level: BBa_K567009
start: 89
end: 1762
orientation: inline

BB123
type: ComponentDefinition
persistent identity: BBa K2023001
display id: BBa_K2023001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor device for BTEX detection
creator: Celia Chenebault, Camille Soucies, Benjamin Piot
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T12:05:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2490_
m user id: 30230
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence.
mutable notes: Site directed mutagenesis to overcome the mutation in the start codon of XylR
mutable provenance: IDT synthesis in 3 parts that were then assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K2023003
component: BBa_K2023002
sequence annotation: BBa_K2023003
sequence annotation: BBa_K2023002
sequence: https://synbiohub.org/public/igem/BBa_K2023001_sequence/1

BBa_K2023003
type: Component
persistent identity: component2518727
display id: component2518727
version: 1
top level: BB123
access: public
definition: BBa_K2023003

BBa_K2023002
type: Component
persistent identity: component2518713
display id: component2518713
version: 1
top level: BB123
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023002/1

BBa_K2023003
type: SequenceAnnotation
persistent identity: annotation2518727
display id: annotation2518727
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
component: BBa_K2023003

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
type: Range
persistent identity: range2518727
display id: range2518727
version: 1
direction: direction/reverse
top level: BB123
start: 2252
end: 3261
orientation: inline

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518713
display id: annotation2518713
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
type: Range
persistent identity: range2518713
display id: range2518713
version: 1
direction: direction/reverse
top level: BB123
start: 1
end: 2243
orientation: inline

BBa_K567003
type: ComponentDefinition
persistent identity: BBa K567003
display id: BBa_K567003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbla-Luc-TAG
creator: Yunfeng Ruan and Chunying Li
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Luciferase under the control of β-lactamase promoter with TAG insertion. A TAG codon is inserted after the initial codon of the gene. This part is used to testify the function of corporation of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).
mutable notes: Point mutation is used to obtain this part from the wild type.
mutable provenance: β-lactamase promoter is derived from pUC18 β-lactamase operon. Wild type luciferase is derived from BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: TAG
sequence annotation: ATG
sequence annotation: luciferase-TAG
sequence annotation: Pbla
sequence: https://synbiohub.org/public/igem/BBa_K567003_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2154546
display id: annotation2154546
version: 1
top level: BBa_K567003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567003/annotation2154546/range2154546/1

https://synbiohub.org/public/igem/BBa_K567003/annotation2154546/range2154546/1
type: Range
persistent identity: range2154546
display id: range2154546
version: 1
direction: direction/forward
top level: BBa_K567003
start: 278
end: 282
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2150723
display id: annotation2150723
version: 1
top level: BBa_K567003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567003/annotation2150723/range2150723/1

https://synbiohub.org/public/igem/BBa_K567003/annotation2150723/range2150723/1
type: Range
persistent identity: range2150723
display id: range2150723
version: 1
direction: direction/forward
top level: BBa_K567003
start: 290
end: 292
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150721
display id: annotation2150721
version: 1
top level: BBa_K567003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567003/annotation2150721/range2150721/1

https://synbiohub.org/public/igem/BBa_K567003/annotation2150721/range2150721/1
type: Range
persistent identity: range2150721
display id: range2150721
version: 1
direction: direction/forward
top level: BBa_K567003
start: 287
end: 289
orientation: inline

luciferase-TAG
type: SequenceAnnotation
persistent identity: annotation2150722
display id: annotation2150722
version: 1
top level: BBa_K567003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567003/annotation2150722/range2150722/1

https://synbiohub.org/public/igem/BBa_K567003/annotation2150722/range2150722/1
type: Range
persistent identity: range2150722
display id: range2150722
version: 1
direction: direction/forward
top level: BBa_K567003
start: 287
end: 1942
orientation: inline

Pbla
type: SequenceAnnotation
persistent identity: annotation2152726
display id: annotation2152726
version: 1
top level: BBa_K567003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567003/annotation2152726/range2152726/1

https://synbiohub.org/public/igem/BBa_K567003/annotation2152726/range2152726/1
type: Range
persistent identity: range2152726
display id: range2152726
version: 1
direction: direction/forward
top level: BBa_K567003
start: 217
end: 244
orientation: inline

BBa_M36012
type: ComponentDefinition
persistent identity: BBa M36012
display id: BBa_M36012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (ideal)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses the glgC, glgA, and glgS proteins/enzymes usually not expressed in e. coli.
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36007
component: BBa_M36724
component: BBa_M36006
component: BBa_M36001
component: BBa_M36008
component: BBa_M36010
component: BBa_M36009
sequence annotation: BBa_M36001
sequence annotation: BBa_M36009
sequence annotation: BBa_M36008
sequence annotation: BBa_M36010
sequence annotation: BBa_M36007
sequence annotation: BBa_M36724
sequence annotation: BBa_M36006
sequence: https://synbiohub.org/public/igem/BBa_M36012_sequence/1

BBa_M36007
type: Component
persistent identity: component2118439
display id: component2118439
version: 1
top level: BBa_M36012
access: public
definition: BBa_M36007

BBa_M36724
type: Component
persistent identity: component2118440
display id: component2118440
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36006
type: Component
persistent identity: component2118437
display id: component2118437
version: 1
top level: BBa_M36012
access: public
definition: BBa_M36006

BBa_M36001
type: Component
persistent identity: component2118438
display id: component2118438
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36001/1

BBa_M36008
type: Component
persistent identity: component2118441
display id: component2118441
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36008/1

BBa_M36010
type: Component
persistent identity: component2118442
display id: component2118442
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36009
type: Component
persistent identity: component2118436
display id: component2118436
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2118438
display id: annotation2118438
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118438/range2118438/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36012/annotation2118438/range2118438/1
type: Range
persistent identity: range2118438
display id: range2118438
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1344
end: 1390
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118436
display id: annotation2118436
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118436/range2118436/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36012/annotation2118436/range2118436/1
type: Range
persistent identity: range2118436
display id: range2118436
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1
end: 47
orientation: inline

BBa_M36008
type: SequenceAnnotation
persistent identity: annotation2118441
display id: annotation2118441
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118441/range2118441/1
component: BBa_M36008

https://synbiohub.org/public/igem/BBa_M36012/annotation2118441/range2118441/1
type: Range
persistent identity: range2118441
display id: range2118441
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 2910
end: 3110
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118442
display id: annotation2118442
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118442/range2118442/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36012/annotation2118442/range2118442/1
type: Range
persistent identity: range2118442
display id: range2118442
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 3111
end: 3192
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2118439
display id: annotation2118439
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118439/range2118439/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36012/annotation2118439/range2118439/1
type: Range
persistent identity: range2118439
display id: range2118439
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1391
end: 2824
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118440
display id: annotation2118440
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118440/range2118440/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36012/annotation2118440/range2118440/1
type: Range
persistent identity: range2118440
display id: range2118440
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 2825
end: 2909
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118437
display id: annotation2118437
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118437/range2118437/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36012/annotation2118437/range2118437/1
type: Range
persistent identity: range2118437
display id: range2118437
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 48
end: 1343
orientation: inline

BBa_K567020
type: ComponentDefinition
persistent identity: BBa K567020
display id: BBa_K567020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KanR
creator: Zhang Kaisi
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The start codon of Kana gene is mutated from ATG to CGA.
mutable provenance: Kana from pET28a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ATG->CGA
sequence annotation: KanR
sequence: https://synbiohub.org/public/igem/BBa_K567020_sequence/1

ATG->CGA
type: SequenceAnnotation
persistent identity: annotation2161660
display id: annotation2161660
version: 1
top level: BBa_K567020
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567020/annotation2161660/range2161660/1

https://synbiohub.org/public/igem/BBa_K567020/annotation2161660/range2161660/1
type: Range
persistent identity: range2161660
display id: range2161660
version: 1
direction: direction/forward
top level: BBa_K567020
start: 1
end: 3
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2161659
display id: annotation2161659
version: 1
top level: BBa_K567020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567020/annotation2161659/range2161659/1

https://synbiohub.org/public/igem/BBa_K567020/annotation2161659/range2161659/1
type: Range
persistent identity: range2161659
display id: range2161659
version: 1
direction: direction/forward
top level: BBa_K567020
start: 1
end: 816
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha fragment
creator: Zhan Jian
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
mutable notes: None
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: STOP
sequence annotation: lacZ
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 229
end: 234
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 234
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 3
orientation: inline

BBa_K216006
type: ComponentDefinition
persistent identity: BBa K216006
display id: BBa_K216006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pentaerythritol tetranitrate reductase with native rbs
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for the enzyme pentaerythritol tetranitrate reductase (PETN reductase) of ''Enterobacter cloacae'' PB2. This enzyme reductively denitrates nitrate esters such as PETN (pentaerythritol tetranitrate, penthrite) GTN (glycerol trinitrate, nitroglycerine) and EGDN (ethylene glycol dinitrate) yielding the alcohol and nitrite ion. It can also reductively attack nitroaromatic compounds such as TNT (2,4,6-trinitrotoluene) forming initially hydride-Meisenheimer complexes which break down via an unknown route to yield nitrite and non-aromatic products. Reference: Binks, P.R., French, C.E., Nicklin, S., & Bruce, N.C. (1996). Degradation of pentaerythritol tetranitrate by ''Enterobacter cloacae'' PB2. Applied and Environmental Microbiology 62(4), 1214-1219. Reference 2: French,C.E., Nicklin,S. and Bruce,N.C. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from ''Enterobacter cloacae'' PB2. J. Bacteriol. 178 (22), 6623-6627 (1996).
mutable notes: No special considerations, but due to an error, the primers were actually designed based on the homologous gene of another strain of ''Enterobacter cloacae'', which has a very similar coding sequence but different sequence in the upstream region. The sequence shown here is the correct sequence determined by sequencing the construct.
mutable provenance: This part was amplified from plasmid pONR1 which bears the PETN reductase gene originally cloned from ''Enterobacter cloacae'' PB2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: stop
sequence annotation: RBS
sequence annotation: PETN reductase coding region
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K216006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2046803
display id: annotation2046803
version: 1
top level: BBa_K216006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046803/range2046803/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046803/range2046803/1
type: Range
persistent identity: range2046803
display id: range2046803
version: 1
direction: direction/forward
top level: BBa_K216006
start: 1104
end: 1109
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2046801
display id: annotation2046801
version: 1
top level: BBa_K216006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046801/range2046801/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046801/range2046801/1
type: Range
persistent identity: range2046801
display id: range2046801
version: 1
direction: direction/forward
top level: BBa_K216006
start: 1
end: 3
orientation: inline

PETN reductase coding region
type: SequenceAnnotation
persistent identity: annotation2046804
display id: annotation2046804
version: 1
top level: BBa_K216006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046804/range2046804/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046804/range2046804/1
type: Range
persistent identity: range2046804
display id: range2046804
version: 1
direction: direction/forward
top level: BBa_K216006
start: 9
end: 1106
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2046802
display id: annotation2046802
version: 1
top level: BBa_K216006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216006/annotation2046802/range2046802/1

https://synbiohub.org/public/igem/BBa_K216006/annotation2046802/range2046802/1
type: Range
persistent identity: range2046802
display id: range2046802
version: 1
direction: direction/forward
top level: BBa_K216006
start: 9
end: 11
orientation: inline

alkB2
type: ComponentDefinition
persistent identity: BBa K398001
display id: BBa_K398001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)
creator: Hugo F. Cueto Rojas and Mathias Voges
created: 2010-06-11T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Alkane 1-monooxygenase from Gordonia sp. TF6
mutable notes: Nucleotide sequence optimized for expression in E.coli
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: AlkB2
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K398001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2070743
display id: annotation2070743
version: 1
top level: alkB2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398001/annotation2070743/range2070743/1

https://synbiohub.org/public/igem/BBa_K398001/annotation2070743/range2070743/1
type: Range
persistent identity: range2070743
display id: range2070743
version: 1
direction: direction/forward
top level: alkB2
start: 1
end: 3
orientation: inline

AlkB2
type: SequenceAnnotation
persistent identity: annotation2070745
display id: annotation2070745
version: 1
top level: alkB2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398001/annotation2070745/range2070745/1

https://synbiohub.org/public/igem/BBa_K398001/annotation2070745/range2070745/1
type: Range
persistent identity: range2070745
display id: range2070745
version: 1
direction: direction/forward
top level: alkB2
start: 1
end: 1225
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2070744
display id: annotation2070744
version: 1
top level: alkB2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398001/annotation2070744/range2070744/1

https://synbiohub.org/public/igem/BBa_K398001/annotation2070744/range2070744/1
type: Range
persistent identity: range2070744
display id: range2070744
version: 1
direction: direction/forward
top level: alkB2
start: 1223
end: 1225
orientation: inline

RBS+OxRed
type: ComponentDefinition
persistent identity: BBa K1313003
display id: BBa_K1313003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Oxoacyl Reductase
creator: Oliva S??nchez
created: 2014-10-11T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 0
owner id: 19941
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite has an associated RBS (BBa_B0034) to BBa_K1313001.
mutable notes: TACTAG scar
mutable provenance: Designed composite by ITESM CEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1313001
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1313001
sequence: https://synbiohub.org/public/igem/BBa_K1313003_sequence/1

BBa_K1313001
type: Component
persistent identity: component2417603
display id: component2417603
version: 1
top level: RBS+OxRed
access: public
definition: BBa_K1313001

BBa_B0034
type: Component
persistent identity: component2417602
display id: component2417602
version: 1
top level: RBS+OxRed
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2417602
display id: annotation2417602
version: 1
top level: RBS+OxRed
location: https://synbiohub.org/public/igem/BBa_K1313003/annotation2417602/range2417602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1313003/annotation2417602/range2417602/1
type: Range
persistent identity: range2417602
display id: range2417602
version: 1
direction: direction/reverse
top level: RBS+OxRed
start: 1
end: 12
orientation: inline

BBa_K1313001
type: SequenceAnnotation
persistent identity: annotation2417603
display id: annotation2417603
version: 1
top level: RBS+OxRed
location: https://synbiohub.org/public/igem/BBa_K1313003/annotation2417603/range2417603/1
component: BBa_K1313001

https://synbiohub.org/public/igem/BBa_K1313003/annotation2417603/range2417603/1
type: Range
persistent identity: range2417603
display id: range2417603
version: 1
direction: direction/reverse
top level: RBS+OxRed
start: 19
end: 960
orientation: inline

BBa_K567007
type: ComponentDefinition
persistent identity: BBa K567007
display id: BBa_K567007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbla-Luc-8AGG
creator: Chunying Li and Yunfeng Ruan
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _735_
m user id: 0
owner id: 8782
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: β-lactamase promoter-Luciferase with 8 AGG-codon insertions. This biobrick is constructed by putting modified enzyme luciferase under constituitive promoter β-lactamase promoter. 8 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of LacI -Ptac-tRNA(Arg)(BBa_K567001) or sulA promoter-tRNA(Arg) (BBa_K567002). Cell is cultured in 50ug/ml kanamycin and 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA. Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes. Amount of bioluminescence produced can be detected using luminometer.
mutable notes: β-lactamase promoter-Luciferase with 6 AGG-codon insertions is derived from BBa_K567006 then point mutation is performed to obtain this part.
mutable provenance: β-lactamase promoter is derived from pUC18 β-lactamase operon. β-lactamase promoter-Luciferase with 6 AGG-codon insertions is derived from BBa_K567006 then point mutation is performed to obtain this part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Pbla
sequence annotation: rbs
sequence annotation: ATG
sequence annotation: luciferase-8AGG
sequence annotation: 8AGG
sequence: https://synbiohub.org/public/igem/BBa_K567007_sequence/1

Pbla
type: SequenceAnnotation
persistent identity: annotation2152729
display id: annotation2152729
version: 1
top level: BBa_K567007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K567007/annotation2152729/range2152729/1

https://synbiohub.org/public/igem/BBa_K567007/annotation2152729/range2152729/1
type: Range
persistent identity: range2152729
display id: range2152729
version: 1
direction: direction/forward
top level: BBa_K567007
start: 217
end: 244
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154568
display id: annotation2154568
version: 1
top level: BBa_K567007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K567007/annotation2154568/range2154568/1

https://synbiohub.org/public/igem/BBa_K567007/annotation2154568/range2154568/1
type: Range
persistent identity: range2154568
display id: range2154568
version: 1
direction: direction/forward
top level: BBa_K567007
start: 278
end: 282
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150764
display id: annotation2150764
version: 1
top level: BBa_K567007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K567007/annotation2150764/range2150764/1

https://synbiohub.org/public/igem/BBa_K567007/annotation2150764/range2150764/1
type: Range
persistent identity: range2150764
display id: range2150764
version: 1
direction: direction/forward
top level: BBa_K567007
start: 288
end: 290
orientation: inline

luciferase-8AGG
type: SequenceAnnotation
persistent identity: annotation2150768
display id: annotation2150768
version: 1
top level: BBa_K567007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K567007/annotation2150768/range2150768/1

https://synbiohub.org/public/igem/BBa_K567007/annotation2150768/range2150768/1
type: Range
persistent identity: range2150768
display id: range2150768
version: 1
direction: direction/forward
top level: BBa_K567007
start: 288
end: 1970
orientation: inline

8AGG
type: SequenceAnnotation
persistent identity: annotation2150765
display id: annotation2150765
version: 1
top level: BBa_K567007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K567007/annotation2150765/range2150765/1

https://synbiohub.org/public/igem/BBa_K567007/annotation2150765/range2150765/1
type: Range
persistent identity: range2150765
display id: range2150765
version: 1
direction: direction/forward
top level: BBa_K567007
start: 291
end: 314
orientation: inline

XynB
type: ComponentDefinition
persistent identity: BBa K1360002
display id: BBa_K1360002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: endo-1,4-beta-xylanase XynB
creator: Liang Jiang
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1736_
m user id: 0
owner id: 22520
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: this gene encodes endo-1,4-beta-xylanase of XynB from Clostridium stercorarium DSM8532, codon optimized for E.coli
mutable notes: codon optimized for E.coli
mutable provenance: whole genome sequencing of Clostridium stercorarium DSM8532
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: XynB
sequence: https://synbiohub.org/public/igem/BBa_K1360002_sequence/1

XynB
type: SequenceAnnotation
persistent identity: annotation2398043
display id: annotation2398043
version: 1
top level: XynB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1360002/annotation2398043/range2398043/1

https://synbiohub.org/public/igem/BBa_K1360002/annotation2398043/range2398043/1
type: Range
persistent identity: range2398043
display id: range2398043
version: 1
direction: direction/forward
top level: XynB
start: 1
end: 1164
orientation: inline

HO1
type: ComponentDefinition
persistent identity: BBa K646000
display id: BBa_K646000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hemeoxygenase with RBS
creator: Edward Moh
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _822_
m user id: 0
owner id: 10455
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
mutable notes: The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation.
mutable provenance: Http://www.ncbi.nlm.nih.gov/gene/953483
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Heme Oxygenase 1
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K646000_sequence/1

Heme Oxygenase 1
type: SequenceAnnotation
persistent identity: annotation2147983
display id: annotation2147983
version: 1
top level: HO1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K646000/annotation2147983/range2147983/1

https://synbiohub.org/public/igem/BBa_K646000/annotation2147983/range2147983/1
type: Range
persistent identity: range2147983
display id: range2147983
version: 1
direction: direction/forward
top level: HO1
start: 22
end: 744
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2147982
display id: annotation2147982
version: 1
top level: HO1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K646000/annotation2147982/range2147982/1

https://synbiohub.org/public/igem/BBa_K646000/annotation2147982/range2147982/1
type: Range
persistent identity: range2147982
display id: range2147982
version: 1
direction: direction/forward
top level: HO1
start: 9
end: 14
orientation: inline

BBa_K1031931
type: ComponentDefinition
persistent identity: BBa K1031931
display id: BBa_K1031931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylC-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylC-TT
mutable notes: PCR & molecular cloning
mutable provenance: Pseudomonas.putida pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: B0015
sequence annotation: XylC
sequence: https://synbiohub.org/public/igem/BBa_K1031931_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2369603
display id: annotation2369603
version: 1
top level: BBa_K1031931
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1
type: Range
persistent identity: range2369603
display id: range2369603
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369605
display id: annotation2369605
version: 1
top level: BBa_K1031931
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1
type: Range
persistent identity: range2369605
display id: range2369605
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1518
end: 1646
orientation: inline

XylC
type: SequenceAnnotation
persistent identity: annotation2369604
display id: annotation2369604
version: 1
top level: BBa_K1031931
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1
type: Range
persistent identity: range2369604
display id: range2369604
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 54
end: 1517
orientation: inline

BBa_K863001
type: ComponentDefinition
persistent identity: BBa K863001
display id: BBa_K863001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul laccase from Bacillus pumilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bpul (Laccase from Bacillus pumilus)
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: A (Original) - T (Mutation)
sequence annotation: bpul
sequence: https://synbiohub.org/public/igem/BBa_K863001_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2184622
display id: annotation2184622
version: 1
top level: BBa_K863001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1
type: Range
persistent identity: range2184622
display id: range2184622
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184641
display id: annotation2184641
version: 1
top level: BBa_K863001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1
type: Range
persistent identity: range2184641
display id: range2184641
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1531
end: 1533
orientation: inline

A (Original) - T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184905
display id: annotation2184905
version: 1
top level: BBa_K863001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1
type: Range
persistent identity: range2184905
display id: range2184905
version: 1
direction: direction/forward
top level: BBa_K863001
start: 801
end: 801
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184621
display id: annotation2184621
version: 1
top level: BBa_K863001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1
type: Range
persistent identity: range2184621
display id: range2184621
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 1533
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
mutable notes: This part is cloned into pSB1A2.
mutable provenance: pSB1AC3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
direction: direction/forward
top level: CmR
start: 1
end: 660
orientation: inline

BBa_K748002
type: ComponentDefinition
persistent identity: BBa K748002
display id: BBa_K748002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lysostaphin is a zinc metalloenzyme that has a specific lytic action against S.aureus. Lysostaphin has activities of three enzymes namely, endo-β-N-acetyl glucosamidase, N-acteyl-muramyl-L-alanine amidase, glycylglycine endopeptidase. Glycylglycine endopeptidase lyses staphylococcal cells by hydrolyzing glycylglycine bonds in the poly-glycine bridges which form cross links between glycopeptide chains in cell wall peptidoglycan of S.aureus cells. The wild-type lysostaphin gene encodes a preproenzyme, and the conversion of prolysostaphin to mature lysostaphin occurs extracellularly and involves the removal of the hydrophilic tandem repeat portion of the proenzyme. In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable notes: In order to directly produce mature lysostaphin, we truncate the preprolysostaphin and prolysostaphin sequence.
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K748002_sequence/1

BBa_K2144001
type: ComponentDefinition
persistent identity: BBa K2144001
display id: BBa_K2144001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for trunctated Lysostaphin regulated by T7-promoter
creator: Oskar ??hman
created: 2016-09-21T11:00:00Z
modified: 2016-09-22T08:03:34Z
discontinued: false
dominant: false
group u list: _2614_
m user id: 31517
owner id: 31517
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coding sequence for truncated Lysostaphin regulated by T7-promoter. This enzyme has the ability to cleave cross-linking pentaglycine bridges in the cell wall peptidoglycan found in certain Staphylococci.
mutable notes: The subunits were aligned by 3A Assembly.
mutable provenance: The sequence encoding for lysostaphin is synthesised de novo according to iGEM Harbin 2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K748002
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K748002
sequence: https://synbiohub.org/public/igem/BBa_K2144001_sequence/1

BBa_K748002
type: Component
persistent identity: component2484492
display id: component2484492
version: 1
top level: BBa_K2144001
access: public
definition: BBa_K748002

BBa_K525998
type: Component
persistent identity: component2484491
display id: component2484491
version: 1
top level: BBa_K2144001
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2484491
display id: annotation2484491
version: 1
top level: BBa_K2144001
location: https://synbiohub.org/public/igem/BBa_K2144001/annotation2484491/range2484491/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K2144001/annotation2484491/range2484491/1
type: Range
persistent identity: range2484491
display id: range2484491
version: 1
direction: direction/reverse
top level: BBa_K2144001
start: 1
end: 32
orientation: inline

BBa_K748002
type: SequenceAnnotation
persistent identity: annotation2484492
display id: annotation2484492
version: 1
top level: BBa_K2144001
location: https://synbiohub.org/public/igem/BBa_K2144001/annotation2484492/range2484492/1
component: BBa_K748002

https://synbiohub.org/public/igem/BBa_K2144001/annotation2484492/range2484492/1
type: Range
persistent identity: range2484492
display id: range2484492
version: 1
direction: direction/reverse
top level: BBa_K2144001
start: 39
end: 779
orientation: inline

BBa_K1462040
type: ComponentDefinition
persistent identity: BBa K1462040
display id: BBa_K1462040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Crt
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1`
mutable provenance: 11
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462040_sequence/1

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly luciferase - luciferase from Photinus pyralis
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
mutable notes: Part is cloned into pSBAK3 vector with terminator.
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

α
type: ComponentDefinition
persistent identity: BBa K336000
display id: BBa_K336000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: α-amylase from Aspergillus oryzae
creator: Yuki Shimahara
created: 2010-10-20T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: true
dominant: false
experience: experience/None
group u list: _458_
m user id: 0
owner id: 5997
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: α-amylase coding parts from Aspergillus oryzae.
mutable notes: None
mutable provenance: EMBL-Bank:X12725.1:Aspergillus oryzae amy1 gene for alpha-amylase (EC 3.2.1.1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K863020
type: ComponentDefinition
persistent identity: BBa K863020
display id: BBa_K863020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans with T7 and HIS
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6 x HIS
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: T7
sequence annotation: ATG
sequence annotation: bhal
sequence: https://synbiohub.org/public/igem/BBa_K863020_sequence/1

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2202255
display id: annotation2202255
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1
type: Range
persistent identity: range2202255
display id: range2202255
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1534
end: 1551
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202257
display id: annotation2202257
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1
type: Range
persistent identity: range2202257
display id: range2202257
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1555
end: 1557
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202252
display id: annotation2202252
version: 1
top level: BBa_K863020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1
type: Range
persistent identity: range2202252
display id: range2202252
version: 1
direction: direction/forward
top level: BBa_K863020
start: 25
end: 30
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2202251
display id: annotation2202251
version: 1
top level: BBa_K863020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1
type: Range
persistent identity: range2202251
display id: range2202251
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2202253
display id: annotation2202253
version: 1
top level: BBa_K863020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1
type: Range
persistent identity: range2202253
display id: range2202253
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 36
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202256
display id: annotation2202256
version: 1
top level: BBa_K863020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1
type: Range
persistent identity: range2202256
display id: range2202256
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1552
end: 1554
orientation: inline

bhal
type: SequenceAnnotation
persistent identity: annotation2202254
display id: annotation2202254
version: 1
top level: BBa_K863020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1
type: Range
persistent identity: range2202254
display id: range2202254
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 1533
orientation: inline

HyaD -> E
type: ComponentDefinition
persistent identity: BBa K1958004
display id: BBa_K1958004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HyaD -> E. coli
creator: Wei Wei
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T09:15:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2425_
m user id: 21319
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: true
mutable description: HyaD is a subunit of hydrogenase 1 (Hyd-1) from Escherichia coli genome. The hya operon, which contains gene for the two HYD1 structual subunits and four additional genes (HyaA-F) was mapped at 22min on the E.coli chromosome. And hyaD is the fourth gene of hya operon, sharing the first few base pairs with hyaC and the last few base pairs with hyaE. HyaD is the specific protease required for large subunit maturation-terminal processing. HyaD is a hydrogenase maturase.
mutable notes: None.
mutable provenance: Escherichia coli BL21
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HyaD coding region
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1958004_sequence/1

HyaD coding region
type: SequenceAnnotation
persistent identity: annotation2493912
display id: annotation2493912
version: 1
top level: HyaD -> E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1958004/annotation2493912/range2493912/1

https://synbiohub.org/public/igem/BBa_K1958004/annotation2493912/range2493912/1
type: Range
persistent identity: range2493912
display id: range2493912
version: 1
direction: direction/forward
top level: HyaD -> E
start: 1
end: 588
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2493911
display id: annotation2493911
version: 1
top level: HyaD -> E
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1958004/annotation2493911/range2493911/1

https://synbiohub.org/public/igem/BBa_K1958004/annotation2493911/range2493911/1
type: Range
persistent identity: range2493911
display id: range2493911
version: 1
direction: direction/forward
top level: HyaD -> E
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2493919
display id: annotation2493919
version: 1
top level: HyaD -> E
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1958004/annotation2493919/range2493919/1

https://synbiohub.org/public/igem/BBa_K1958004/annotation2493919/range2493919/1
type: Range
persistent identity: range2493919
display id: range2493919
version: 1
direction: direction/forward
top level: HyaD -> E
start: 586
end: 588
orientation: inline

BBa_K1660000
type: ComponentDefinition
persistent identity: BBa K1660000
display id: BBa_K1660000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bace16
creator: Dai Yuanyi
created: 2015-09-04T11:00:00Z
modified: 2015-09-07T06:24:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2078_
m user id: 26933
owner id: 24912
owning group id: 9
status: status/Planning
bookmark: false
mutable description: It is toxin to namatodes.
mutable notes: In order to have a better expression in E. coli, we optimised the coding sequence of rMpL, and add an L- arabinose induced promoter pBAD.
mutable provenance: It comes from the Macrolepiota procera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K206000
sequence annotation: stop codon
sequence annotation: bace16
sequence annotation: start codon
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1660000_sequence/1

K206000
type: SequenceAnnotation
persistent identity: annotation2445458
display id: annotation2445458
version: 1
top level: BBa_K1660000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660000/annotation2445458/range2445458/1

https://synbiohub.org/public/igem/BBa_K1660000/annotation2445458/range2445458/1
type: Range
persistent identity: range2445458
display id: range2445458
version: 1
direction: direction/forward
top level: BBa_K1660000
start: 1
end: 130
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445462
display id: annotation2445462
version: 1
top level: BBa_K1660000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660000/annotation2445462/range2445462/1

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type: Range
persistent identity: range2445462
display id: range2445462
version: 1
direction: direction/forward
top level: BBa_K1660000
start: 1303
end: 1305
orientation: inline

bace16
type: SequenceAnnotation
persistent identity: annotation2445461
display id: annotation2445461
version: 1
top level: BBa_K1660000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660000/annotation2445461/range2445461/1

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type: Range
persistent identity: range2445461
display id: range2445461
version: 1
direction: direction/forward
top level: BBa_K1660000
start: 157
end: 1305
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445460
display id: annotation2445460
version: 1
top level: BBa_K1660000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660000/annotation2445460/range2445460/1

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type: Range
persistent identity: range2445460
display id: range2445460
version: 1
direction: direction/forward
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start: 157
end: 159
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B0034
type: SequenceAnnotation
persistent identity: annotation2445459
display id: annotation2445459
version: 1
top level: BBa_K1660000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660000/annotation2445459/range2445459/1

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type: Range
persistent identity: range2445459
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start: 139
end: 150
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BBa_K849003
type: ComponentDefinition
persistent identity: BBa K849003
display id: BBa_K849003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bifunctional Cyclase from Gibberella fujikuroi
creator: Martin Schneider, Sebastian Roy
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1109_
m user id: 0
owner id: 12313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CPS/KS
mutable notes: no
mutable provenance: Genomic sequence of Gibberella fujikuroi.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K849003_sequence/1

cds
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persistent identity: annotation2202327
display id: annotation2202327
version: 1
top level: BBa_K849003
role: feature/cds
role: SO:0000316
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persistent identity: range2202327
display id: range2202327
version: 1
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start: 1
end: 2859
orientation: inline