//cds/biosynthesis/anthocyanins
type: Collection
persistent identity: cds biosynthesis anthocyanins
display id: cds_biosynthesis_anthocyanins
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1497003
member: BBa_K1497006
member: BBa_K1497017
member: BBa_K1497004
member: BBa_K1497011
member: BBa_K1497013
member: BBa_K1497000
member: BBa_K1497015
member: BBa_K1497002
member: BBa_K1497010
member: BBa_K1497009
member: BBa_K1497023
member: BBa_K1497007
member: BBa_K1497005
member: BBa_K1497012
member: BBa_K1497018
member: BBa_K1497001
member: BBa_K1497016
member: BBa_K1497014

//cds/biosynthesis
type: Collection
persistent identity: cds biosynthesis
display id: cds_biosynthesis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1462060
member: RcsB
member: BBa_K1497017
member: BBa_K801070
member: BBa_K1497010
member: BBa_M36008
member: BBa_K1462030
member: BBa_K1149002
member: BBa_K1497005
member: BBa_M36013
member: BBa_K1497001
member: BBa_K424017
member: test
member: lipA
member: BBa_K1660001
member: HO1
member: BBa_K1554002
member: pET fusion
member: BBa_K394003
member: BBa_K518009
member: norC
member: BBa_K801074
member: BBa_K1404008
member: BBa_K552001
member: BBa_K801091
member: BBa_K801061
member: BBa_K1172305
member: DGAT
member: BBa_K1067004
member: BBa_K1462260
member: BBa_K1751000
member: BBa_K808011
member: BBa_K801073
member: BBa_K801098
member: BBa_K1404007
member: B-carotene
member: BBa_M36007
member: cry11Aa
member: BBa_K1331001
member: BBa_K1315004
member: BBa_K552002
member: BBa_K1122676
member: BBa_K2042010
member: BBa_M36002
member: BBa_K801071
member: BBa_K1152001
member: BBa_K801080
member: BBa_K1497011
member: BBa_K1660009
member: BBa_K801097
member: BBa_K1497002
member: BBa_K1497009
member: BBa_K1554003
member: BBa_K108015
member: LipA
member: BBa_K1687000
member: BBa_K332031
member: BBa_K1172588
member: BBa_K1497018
member: BBa_K2042012
member: BBa_K801095
member: BBa_K1152008
member: BBa_K1598001
member: BBa_M36004
member: BBa_K1497013
member: BBa_K1331004
member: BBa_K1497000
member: nosZ
member: BBa_K1486031
member: BBa_K646003
member: BBa_K1497007
member: BBa_K2042001

BBa_K1486031
type: ComponentDefinition
persistent identity: BBa K1486031
display id: BBa_K1486031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaUra3 selection marker
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: TEF terminator
sequence annotation: Ura3
sequence annotation: TEF promoter
sequence: https://synbiohub.org/public/igem/BBa_K1486031_sequence/1

TEF terminator
type: SequenceAnnotation
persistent identity: annotation2417787
display id: annotation2417787
version: 1
top level: BBa_K1486031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417787/range2417787/1
type: Range
persistent identity: range2417787
display id: range2417787
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1162
end: 1360
orientation: inline

Ura3
type: SequenceAnnotation
persistent identity: annotation2417786
display id: annotation2417786
version: 1
top level: BBa_K1486031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417786/range2417786/1
type: Range
persistent identity: range2417786
display id: range2417786
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 345
end: 1157
orientation: inline

TEF promoter
type: SequenceAnnotation
persistent identity: annotation2417785
display id: annotation2417785
version: 1
top level: BBa_K1486031
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1

https://synbiohub.org/public/igem/BBa_K1486031/annotation2417785/range2417785/1
type: Range
persistent identity: range2417785
display id: range2417785
version: 1
direction: direction/forward
top level: BBa_K1486031
start: 1
end: 344
orientation: inline

BBa_K108019
type: ComponentDefinition
persistent identity: BBa K108019
display id: BBa_K108019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhbC
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming
mutable notes: PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 and Not1 using site-directed mutation.
mutable provenance: pBHR68
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phbC
sequence: https://synbiohub.org/public/igem/BBa_K108019_sequence/1

phbC
type: SequenceAnnotation
persistent identity: annotation1986974
display id: annotation1986974
version: 1
top level: BBa_K108019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108019/annotation1986974/range1986974/1

https://synbiohub.org/public/igem/BBa_K108019/annotation1986974/range1986974/1
type: Range
persistent identity: range1986974
display id: range1986974
version: 1
direction: direction/forward
top level: BBa_K108019
start: 1
end: 1767
orientation: inline

BBa_K801064
type: ComponentDefinition
persistent identity: BBa K801064
display id: BBa_K801064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express ''Citrus limon'' (+)-limonene synthase 1 in yeast. The expression is controled by the yeast TEF2 promoter and the yeast CYC1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF2 promoter (BBa_K801010), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K122003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Cyc1 terminator
sequence annotation: stop codon
sequence annotation: Age1 restriction site
sequence annotation: TEF2 promotor
sequence annotation: BBa_K801061
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801064_sequence/1

Cyc1 terminator
type: SequenceAnnotation
persistent identity: annotation2197890
display id: annotation2197890
version: 1
top level: BBa_K801064
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197890/range2197890/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197890/range2197890/1
type: Range
persistent identity: range2197890
display id: range2197890
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2336
end: 2618
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2197892
display id: annotation2197892
version: 1
top level: BBa_K801064
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197892/range2197892/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197892/range2197892/1
type: Range
persistent identity: range2197892
display id: range2197892
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2326
end: 2328
orientation: inline

Age1 restriction site
type: SequenceAnnotation
persistent identity: annotation2197891
display id: annotation2197891
version: 1
top level: BBa_K801064
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197891/range2197891/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197891/range2197891/1
type: Range
persistent identity: range2197891
display id: range2197891
version: 1
direction: direction/forward
top level: BBa_K801064
start: 2320
end: 2325
orientation: inline

TEF2 promotor
type: SequenceAnnotation
persistent identity: annotation2197889
display id: annotation2197889
version: 1
top level: BBa_K801064
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197889/range2197889/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197889/range2197889/1
type: Range
persistent identity: range2197889
display id: range2197889
version: 1
direction: direction/forward
top level: BBa_K801064
start: 1
end: 649
orientation: inline

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197887
display id: annotation2197887
version: 1
top level: BBa_K801064
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197887/range2197887/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197887/range2197887/1
type: Range
persistent identity: range2197887
display id: range2197887
version: 1
direction: direction/reverse
top level: BBa_K801064
start: 655
end: 2319
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197886
display id: annotation2197886
version: 1
top level: BBa_K801064
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801064/annotation2197886/range2197886/1

https://synbiohub.org/public/igem/BBa_K801064/annotation2197886/range2197886/1
type: Range
persistent identity: range2197886
display id: range2197886
version: 1
direction: direction/forward
top level: BBa_K801064
start: 655
end: 2319
orientation: inline

BBa_K1152001
type: ComponentDefinition
persistent identity: BBa K1152001
display id: BBa_K1152001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TycB1 Module from Tyrocidine
creator: Tania Christiansen
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
group u list: _1464_
m user id: 0
owner id: 16899
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the CDS for TycB1 module from the Tyrocidine gene cluster of Brevibacillus Parabrevis. It is the second module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine. This specific module TycB1 is responsiple for the binding of the second amino acid Proline of the NRP. It consists of the C- (condensation), A- (adenylation) and the PCP- (peptide-carrier-protein) domain. After Tyc B1 follows the TycB2 module of the TycB gene which has three modules. This bio brick can be used for the design of different NRPS.
mutable notes: Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine).
mutable provenance: Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: Coding Sequence TycB1
sequence: https://synbiohub.org/public/igem/BBa_K1152001_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2352396
display id: annotation2352396
version: 1
top level: BBa_K1152001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1152001/annotation2352396/range2352396/1

https://synbiohub.org/public/igem/BBa_K1152001/annotation2352396/range2352396/1
type: Range
persistent identity: range2352396
display id: range2352396
version: 1
direction: direction/forward
top level: BBa_K1152001
start: 1
end: 3
orientation: inline

Coding Sequence TycB1
type: SequenceAnnotation
persistent identity: annotation2352395
display id: annotation2352395
version: 1
top level: BBa_K1152001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1152001/annotation2352395/range2352395/1

https://synbiohub.org/public/igem/BBa_K1152001/annotation2352395/range2352395/1
type: Range
persistent identity: range2352395
display id: range2352395
version: 1
direction: direction/forward
top level: BBa_K1152001
start: 1
end: 3153
orientation: inline

BBa_K1462260
type: ComponentDefinition
persistent identity: BBa K1462260
display id: BBa_K1462260
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal1+cox6+Erg10+cyc1
creator: Chuyao Fan
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:35Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 111
mutable notes: 111
mutable provenance: Erg10 encodes thiolase in Saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1462070
component: BBa_J63006
component: BBa_K1462020
component: BBa_K1462000
sequence annotation: BBa_K1462000
sequence annotation: BBa_J63006
sequence annotation: BBa_K1462020
sequence annotation: BBa_K1462070
sequence: https://synbiohub.org/public/igem/BBa_K1462260_sequence/1

BBa_K1462070
type: Component
persistent identity: component2426153
display id: component2426153
version: 1
top level: BBa_K1462260
access: public
definition: https://synbiohub.org/public/igem/BBa_K1462070/1

BBa_J63006
type: Component
persistent identity: component2426150
display id: component2426150
version: 1
top level: BBa_K1462260
access: public
definition: https://synbiohub.org/public/igem/BBa_J63006/1

BBa_K1462020
type: Component
persistent identity: component2426152
display id: component2426152
version: 1
top level: BBa_K1462260
access: public
definition: BBa_K1462020

BBa_K1462000
type: Component
persistent identity: component2426151
display id: component2426151
version: 1
top level: BBa_K1462260
access: public
definition: https://synbiohub.org/public/igem/BBa_K1462000/1

BBa_K1462000
type: SequenceAnnotation
persistent identity: annotation2426151
display id: annotation2426151
version: 1
top level: BBa_K1462260
location: https://synbiohub.org/public/igem/BBa_K1462260/annotation2426151/range2426151/1
component: BBa_K1462000

https://synbiohub.org/public/igem/BBa_K1462260/annotation2426151/range2426151/1
type: Range
persistent identity: range2426151
display id: range2426151
version: 1
direction: direction/reverse
top level: BBa_K1462260
start: 556
end: 675
orientation: inline

BBa_J63006
type: SequenceAnnotation
persistent identity: annotation2426150
display id: annotation2426150
version: 1
top level: BBa_K1462260
location: https://synbiohub.org/public/igem/BBa_K1462260/annotation2426150/range2426150/1
component: BBa_J63006

https://synbiohub.org/public/igem/BBa_K1462260/annotation2426150/range2426150/1
type: Range
persistent identity: range2426150
display id: range2426150
version: 1
direction: direction/reverse
top level: BBa_K1462260
start: 1
end: 549
orientation: inline

BBa_K1462020
type: SequenceAnnotation
persistent identity: annotation2426152
display id: annotation2426152
version: 1
top level: BBa_K1462260
location: https://synbiohub.org/public/igem/BBa_K1462260/annotation2426152/range2426152/1
component: BBa_K1462020

https://synbiohub.org/public/igem/BBa_K1462260/annotation2426152/range2426152/1
type: Range
persistent identity: range2426152
display id: range2426152
version: 1
direction: direction/reverse
top level: BBa_K1462260
start: 682
end: 1878
orientation: inline

BBa_K1462070
type: SequenceAnnotation
persistent identity: annotation2426153
display id: annotation2426153
version: 1
top level: BBa_K1462260
location: https://synbiohub.org/public/igem/BBa_K1462260/annotation2426153/range2426153/1
component: BBa_K1462070

https://synbiohub.org/public/igem/BBa_K1462260/annotation2426153/range2426153/1
type: Range
persistent identity: range2426153
display id: range2426153
version: 1
direction: direction/reverse
top level: BBa_K1462260
start: 1887
end: 2136
orientation: inline

BBa_K1172588
type: ComponentDefinition
persistent identity: BBa K1172588
display id: BBa_K1172588
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Combination of oprF and rib-synth coding sequence under control of T7 promoter
creator: Thorben Meyer
created: 2013-10-24T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172502
component: BBa_K1172303
sequence annotation: BBa_K1172502
sequence annotation: BBa_K1172303
sequence: https://synbiohub.org/public/igem/BBa_K1172588_sequence/1

BBa_K1172502
type: Component
persistent identity: component2370606
display id: component2370606
version: 1
top level: BBa_K1172588
access: public
definition: https://synbiohub.org/public/igem/BBa_K1172502/1

BBa_K1172303
type: Component
persistent identity: component2370611
display id: component2370611
version: 1
top level: BBa_K1172588
access: public
definition: rib-synth

BBa_K1172502
type: SequenceAnnotation
persistent identity: annotation2370606
display id: annotation2370606
version: 1
top level: BBa_K1172588
location: https://synbiohub.org/public/igem/BBa_K1172588/annotation2370606/range2370606/1
component: BBa_K1172502

https://synbiohub.org/public/igem/BBa_K1172588/annotation2370606/range2370606/1
type: Range
persistent identity: range2370606
display id: range2370606
version: 1
direction: direction/reverse
top level: BBa_K1172588
start: 1
end: 1336
orientation: inline

BBa_K1172303
type: SequenceAnnotation
persistent identity: annotation2370611
display id: annotation2370611
version: 1
top level: BBa_K1172588
location: https://synbiohub.org/public/igem/BBa_K1172588/annotation2370611/range2370611/1
component: BBa_K1172303

https://synbiohub.org/public/igem/BBa_K1172588/annotation2370611/range2370611/1
type: Range
persistent identity: range2370611
display id: range2370611
version: 1
direction: direction/reverse
top level: BBa_K1172588
start: 1343
end: 4939
orientation: inline

Pct
type: ComponentDefinition
persistent identity: BBa K2042000
display id: BBa_K2042000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Propionate CoA transferase for P. putida
creator: Toky RATOVOMANANA
created: 2016-08-18T11:00:00Z
modified: 2016-10-15T05:56:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the DNA coding sequence for the enzyme Propionate CoA. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. Transferase. This mutated sequence has one amino acid substitution: A243T (compared to the wild type enzyme) and is codon optimized for Pseudomonas putida in order to produce (D)-Lactyl-CoA. It extend the BBa_K1211001 biobrick from Yale 2013 and have the same purpose.
mutable notes: We use this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA.
mutable provenance: The wild type sequence is from Clostridium propionicum genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: s_mutation
sequence annotation: mutation
sequence annotation: Stop
sequence annotation: Start
sequence annotation: Pct
sequence: https://synbiohub.org/public/igem/BBa_K2042000_sequence/1

s_mutation
type: SequenceAnnotation
persistent identity: annotation2512231
display id: annotation2512231
version: 1
top level: Pct
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512231/range2512231/1

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512231/range2512231/1
type: Range
persistent identity: range2512231
display id: range2512231
version: 1
direction: direction/forward
top level: Pct
start: 1446
end: 1447
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2512235
display id: annotation2512235
version: 1
top level: Pct
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512235/range2512235/1

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type: Range
persistent identity: range2512235
display id: range2512235
version: 1
direction: direction/forward
top level: Pct
start: 727
end: 728
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2512229
display id: annotation2512229
version: 1
top level: Pct
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512229/range2512229/1

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512229/range2512229/1
type: Range
persistent identity: range2512229
display id: range2512229
version: 1
direction: direction/forward
top level: Pct
start: 1573
end: 1575
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512228
display id: annotation2512228
version: 1
top level: Pct
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512228/range2512228/1

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512228/range2512228/1
type: Range
persistent identity: range2512228
display id: range2512228
version: 1
direction: direction/forward
top level: Pct
start: 1
end: 3
orientation: inline

Pct
type: SequenceAnnotation
persistent identity: annotation2512227
display id: annotation2512227
version: 1
top level: Pct
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2042000/annotation2512227/range2512227/1

https://synbiohub.org/public/igem/BBa_K2042000/annotation2512227/range2512227/1
type: Range
persistent identity: range2512227
display id: range2512227
version: 1
direction: direction/forward
top level: Pct
start: 1
end: 1575
orientation: inline

BBa_K1497014
type: ComponentDefinition
persistent identity: BBa K1497014
display id: BBa_K1497014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon (low yield)- T7-B0034-F3H-B0034-DFR-B0034-ANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: s
mutable notes: s
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497013
component: BBa_I712074
component: BBa_K1497011
component: BBa_K1497012
sequence annotation: BBa_I712074
sequence annotation: BBa_K1497011
sequence annotation: BBa_K1497013
sequence annotation: BBa_K1497012
sequence: https://synbiohub.org/public/igem/BBa_K1497014_sequence/1

BBa_K1497013
type: Component
persistent identity: component2398856
display id: component2398856
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497013

BBa_I712074
type: Component
persistent identity: component2398848
display id: component2398848
version: 1
top level: BBa_K1497014
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K1497011
type: Component
persistent identity: component2398857
display id: component2398857
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497011

BBa_K1497012
type: Component
persistent identity: component2398852
display id: component2398852
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497012

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2398848
display id: annotation2398848
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398848/range2398848/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1497014/annotation2398848/range2398848/1
type: Range
persistent identity: range2398848
display id: range2398848
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 1
end: 46
orientation: inline

BBa_K1497011
type: SequenceAnnotation
persistent identity: annotation2398857
display id: annotation2398857
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398857/range2398857/1
component: BBa_K1497011

https://synbiohub.org/public/igem/BBa_K1497014/annotation2398857/range2398857/1
type: Range
persistent identity: range2398857
display id: range2398857
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 2276
end: 3445
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2398856
display id: annotation2398856
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398856/range2398856/1
component: BBa_K1497013

https://synbiohub.org/public/igem/BBa_K1497014/annotation2398856/range2398856/1
type: Range
persistent identity: range2398856
display id: range2398856
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 1188
end: 2267
orientation: inline

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2398852
display id: annotation2398852
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398852/range2398852/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497014/annotation2398852/range2398852/1
type: Range
persistent identity: range2398852
display id: range2398852
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 55
end: 1179
orientation: inline

BBa_K1067003
type: ComponentDefinition
persistent identity: BBa K1067003
display id: BBa_K1067003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ammonium monooxygenase from Nitrosomonas europaea
creator: Kristian Davidsen and Helen Cook
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: hjjkl
mutable notes: uiop
mutable provenance: jhkjl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: amoB
sequence annotation: amoA
sequence annotation: amoC
sequence: https://synbiohub.org/public/igem/BBa_K1067003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2359440
display id: annotation2359440
version: 1
top level: BBa_K1067003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359440/range2359440/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359440/range2359440/1
type: Range
persistent identity: range2359440
display id: range2359440
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 1
end: 3
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359447
display id: annotation2359447
version: 1
top level: BBa_K1067003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359447/range2359447/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359447/range2359447/1
type: Range
persistent identity: range2359447
display id: range2359447
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 1812
end: 1814
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359445
display id: annotation2359445
version: 1
top level: BBa_K1067003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359445/range2359445/1

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type: Range
persistent identity: range2359445
display id: range2359445
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 1808
end: 1810
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359444
display id: annotation2359444
version: 1
top level: BBa_K1067003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359444/range2359444/1

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type: Range
persistent identity: range2359444
display id: range2359444
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 980
end: 982
orientation: inline

amoB
type: SequenceAnnotation
persistent identity: annotation2359446
display id: annotation2359446
version: 1
top level: BBa_K1067003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359446/range2359446/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359446/range2359446/1
type: Range
persistent identity: range2359446
display id: range2359446
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 1812
end: 3074
orientation: inline

amoA
type: SequenceAnnotation
persistent identity: annotation2359442
display id: annotation2359442
version: 1
top level: BBa_K1067003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359442/range2359442/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359442/range2359442/1
type: Range
persistent identity: range2359442
display id: range2359442
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 980
end: 1810
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359448
display id: annotation2359448
version: 1
top level: BBa_K1067003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359448/range2359448/1

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type: Range
persistent identity: range2359448
display id: range2359448
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 3072
end: 3074
orientation: inline

amoC
type: SequenceAnnotation
persistent identity: annotation2359441
display id: annotation2359441
version: 1
top level: BBa_K1067003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359441/range2359441/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359441/range2359441/1
type: Range
persistent identity: range2359441
display id: range2359441
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 1
end: 816
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359443
display id: annotation2359443
version: 1
top level: BBa_K1067003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067003/annotation2359443/range2359443/1

https://synbiohub.org/public/igem/BBa_K1067003/annotation2359443/range2359443/1
type: Range
persistent identity: range2359443
display id: range2359443
version: 1
direction: direction/forward
top level: BBa_K1067003
start: 814
end: 816
orientation: inline

BBa_K552001
type: ComponentDefinition
persistent identity: BBa K552001
display id: BBa_K552001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtE Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-23T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the enzyme CrtE, also known as geranylgeranyl diphosphate synthase. CrtE serves as the first step in carotenoid biosynthesis in Saccharomyces cerevisiae by converting farnesyl diphosphate (which is naturally found in yeast) to geranylgeranyl diphosphate.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start Codon
sequence annotation: CrtE
sequence annotation: ADH1 Terminator
sequence annotation: Stop Codon
sequence annotation: PGK Promoter
sequence: https://synbiohub.org/public/igem/BBa_K552001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2139159
display id: annotation2139159
version: 1
top level: BBa_K552001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139159/range2139159/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139159/range2139159/1
type: Range
persistent identity: range2139159
display id: range2139159
version: 1
direction: direction/forward
top level: BBa_K552001
start: 381
end: 383
orientation: inline

CrtE
type: SequenceAnnotation
persistent identity: annotation2139160
display id: annotation2139160
version: 1
top level: BBa_K552001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139160/range2139160/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139160/range2139160/1
type: Range
persistent identity: range2139160
display id: range2139160
version: 1
direction: direction/forward
top level: BBa_K552001
start: 381
end: 1511
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2139162
display id: annotation2139162
version: 1
top level: BBa_K552001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139162/range2139162/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139162/range2139162/1
type: Range
persistent identity: range2139162
display id: range2139162
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1512
end: 1639
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2139161
display id: annotation2139161
version: 1
top level: BBa_K552001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139161/range2139161/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139161/range2139161/1
type: Range
persistent identity: range2139161
display id: range2139161
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1508
end: 1510
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2139158
display id: annotation2139158
version: 1
top level: BBa_K552001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552001/annotation2139158/range2139158/1

https://synbiohub.org/public/igem/BBa_K552001/annotation2139158/range2139158/1
type: Range
persistent identity: range2139158
display id: range2139158
version: 1
direction: direction/forward
top level: BBa_K552001
start: 1
end: 380
orientation: inline

BBa_K1497007
type: ComponentDefinition
persistent identity: BBa K1497007
display id: BBa_K1497007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin producing operon
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497100) CHS (Bba_K1497101) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.
mutable notes: no
mutable provenance: The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497001
component: BBa_K1497000
component: BBa_B0034
component: BBa_K1033000
component: BBa_K801093
sequence annotation: BBa_K801093
sequence annotation: BBa_B0034
sequence annotation: BBa_K1497001
sequence annotation: BBa_K1033000
sequence annotation: BBa_K1497000
sequence: https://synbiohub.org/public/igem/BBa_K1497007_sequence/1

BBa_K1497001
type: Component
persistent identity: component2398762
display id: component2398762
version: 1
top level: BBa_K1497007
access: public
definition: BBa_K1497001

BBa_K1497000
type: Component
persistent identity: component2398759
display id: component2398759
version: 1
top level: BBa_K1497007
access: public
definition: BBa_K1497000

BBa_B0034
type: Component
persistent identity: component2398761
display id: component2398761
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2398747
display id: component2398747
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2398753
display id: component2398753
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1033000
type: Component
persistent identity: component2398756
display id: component2398756
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033000/1

BBa_K801093
type: Component
persistent identity: component2398751
display id: component2398751
version: 1
top level: BBa_K1497007
access: public
definition: BBa_K801093

BBa_B0034
type: Component
persistent identity: component2398758
display id: component2398758
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K801093
type: SequenceAnnotation
persistent identity: annotation2398751
display id: annotation2398751
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
component: BBa_K801093

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
type: Range
persistent identity: range2398751
display id: range2398751
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 19
end: 1704
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398753
display id: annotation2398753
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398753/range2398753/1
component: BBa_B0034

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type: Range
persistent identity: range2398753
display id: range2398753
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1713
end: 1724
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398758
display id: annotation2398758
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
type: Range
persistent identity: range2398758
display id: range2398758
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 3337
end: 3348
orientation: inline

BBa_K1497001
type: SequenceAnnotation
persistent identity: annotation2398762
display id: annotation2398762
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
component: BBa_K1497001

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
type: Range
persistent identity: range2398762
display id: range2398762
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 4107
end: 5303
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398747
display id: annotation2398747
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
type: Range
persistent identity: range2398747
display id: range2398747
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1
end: 12
orientation: inline

BBa_K1033000
type: SequenceAnnotation
persistent identity: annotation2398756
display id: annotation2398756
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
component: BBa_K1033000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
type: Range
persistent identity: range2398756
display id: range2398756
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1733
end: 3328
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398761
display id: annotation2398761
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
type: Range
persistent identity: range2398761
display id: range2398761
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 4089
end: 4100
orientation: inline

BBa_K1497000
type: SequenceAnnotation
persistent identity: annotation2398759
display id: annotation2398759
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
component: BBa_K1497000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
type: Range
persistent identity: range2398759
display id: range2398759
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 3355
end: 4080
orientation: inline

BBa_M36003
type: ComponentDefinition
persistent identity: BBa M36003
display id: BBa_M36003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glycogen synthase (w/UTR&term)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details.
mutable provenance: Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_M36010
component: BBa_M36007
component: BBa_M36009
sequence annotation: BBa_M36009
sequence annotation: BBa_M36007
sequence annotation: BBa_M36010
sequence: https://synbiohub.org/public/igem/BBa_M36003_sequence/1

BBa_M36010
type: Component
persistent identity: component2117842
display id: component2117842
version: 1
top level: BBa_M36003
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36007
type: Component
persistent identity: component2117841
display id: component2117841
version: 1
top level: BBa_M36003
access: public
definition: BBa_M36007

BBa_M36009
type: Component
persistent identity: component2117840
display id: component2117840
version: 1
top level: BBa_M36003
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2117840
display id: annotation2117840
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117840/range2117840/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36003/annotation2117840/range2117840/1
type: Range
persistent identity: range2117840
display id: range2117840
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 1
end: 47
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2117841
display id: annotation2117841
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117841/range2117841/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36003/annotation2117841/range2117841/1
type: Range
persistent identity: range2117841
display id: range2117841
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 48
end: 1481
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2117842
display id: annotation2117842
version: 1
top level: BBa_M36003
location: https://synbiohub.org/public/igem/BBa_M36003/annotation2117842/range2117842/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36003/annotation2117842/range2117842/1
type: Range
persistent identity: range2117842
display id: range2117842
version: 1
direction: direction/reverse
top level: BBa_M36003
start: 1482
end: 1563
orientation: inline

BBa_K552002
type: ComponentDefinition
persistent identity: BBa K552002
display id: BBa_K552002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtYB Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the binary enzyme CrtYB. CrtY (Lycopene Beta-cyclase) converts lycopene into beta-carotene and serves as the last step in beta-carotene biosynthesis in Saccharomyces cerevisiae. CrtB is acts further upstream of CrtY in the pathway and is called Phytoene Synthase. CrtB converts geranylgeranyl diphosphate to phytoene.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: PGK Promoter
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence annotation: CrtYB
sequence annotation: ADH1 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K552002_sequence/1

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145064
display id: annotation2145064
version: 1
top level: BBa_K552002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145064/range2145064/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145064/range2145064/1
type: Range
persistent identity: range2145064
display id: range2145064
version: 1
direction: direction/forward
top level: BBa_K552002
start: 1
end: 380
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145065
display id: annotation2145065
version: 1
top level: BBa_K552002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145065/range2145065/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145065/range2145065/1
type: Range
persistent identity: range2145065
display id: range2145065
version: 1
direction: direction/forward
top level: BBa_K552002
start: 381
end: 383
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145067
display id: annotation2145067
version: 1
top level: BBa_K552002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145067/range2145067/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145067/range2145067/1
type: Range
persistent identity: range2145067
display id: range2145067
version: 1
direction: direction/forward
top level: BBa_K552002
start: 2400
end: 2402
orientation: inline

CrtYB
type: SequenceAnnotation
persistent identity: annotation2145066
display id: annotation2145066
version: 1
top level: BBa_K552002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145066/range2145066/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145066/range2145066/1
type: Range
persistent identity: range2145066
display id: range2145066
version: 1
direction: direction/forward
top level: BBa_K552002
start: 383
end: 2402
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145068
display id: annotation2145068
version: 1
top level: BBa_K552002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552002/annotation2145068/range2145068/1

https://synbiohub.org/public/igem/BBa_K552002/annotation2145068/range2145068/1
type: Range
persistent identity: range2145068
display id: range2145068
version: 1
direction: direction/forward
top level: BBa_K552002
start: 2403
end: 2531
orientation: inline

DGAT
type: ComponentDefinition
persistent identity: BBa K836002
display id: BBa_K836002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids. Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT.
mutable notes: Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.
mutable provenance: The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K836001
component: BBa_B1006
sequence annotation: BBa_K836001
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K836002_sequence/1

BBa_K836001
type: Component
persistent identity: component2185269
display id: component2185269
version: 1
top level: DGAT
access: public
definition: DGAT

BBa_B1006
type: Component
persistent identity: component2185274
display id: component2185274
version: 1
top level: DGAT
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B1006
type: Component
persistent identity: component2185279
display id: component2185279
version: 1
top level: DGAT
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K836001
type: SequenceAnnotation
persistent identity: annotation2185269
display id: annotation2185269
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185269/range2185269/1
component: BBa_K836001

https://synbiohub.org/public/igem/BBa_K836002/annotation2185269/range2185269/1
type: Range
persistent identity: range2185269
display id: range2185269
version: 1
direction: direction/reverse
top level: DGAT
start: 1
end: 1380
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185279
display id: annotation2185279
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185279/range2185279/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836002/annotation2185279/range2185279/1
type: Range
persistent identity: range2185279
display id: range2185279
version: 1
direction: direction/reverse
top level: DGAT
start: 1420
end: 1458
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185274
display id: annotation2185274
version: 1
top level: DGAT
location: https://synbiohub.org/public/igem/BBa_K836002/annotation2185274/range2185274/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836002/annotation2185274/range2185274/1
type: Range
persistent identity: range2185274
display id: range2185274
version: 1
direction: direction/reverse
top level: DGAT
start: 1381
end: 1419
orientation: inline

BBa_K108015
type: ComponentDefinition
persistent identity: BBa K108015
display id: BBa_K108015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhaR
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 and EcoR1 using site-directed mutation.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PhaR
sequence: https://synbiohub.org/public/igem/BBa_K108015_sequence/1

PhaR
type: SequenceAnnotation
persistent identity: annotation1986960
display id: annotation1986960
version: 1
top level: BBa_K108015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108015/annotation1986960/range1986960/1

https://synbiohub.org/public/igem/BBa_K108015/annotation1986960/range1986960/1
type: Range
persistent identity: range1986960
display id: range1986960
version: 1
direction: direction/forward
top level: BBa_K108015
start: 1
end: 549
orientation: inline

BBa_K1497017
type: ComponentDefinition
persistent identity: BBa K1497017
display id: BBa_K1497017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin producing operon under the T7 promoter BBa_I712074
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Transformed into BL21(DE3) and induced with IPTG this composite is able to produce naringenin from tyrosine. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497000) CHS (BBa_K1497001) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented. For characterization the naringenin biosensor K1497020 was used. After 24 h incubation GFP fluorescence was visible to the unaided eye. We measured the relative GFP fluorescence by use of OptimaFluoroStar.
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497007
component: BBa_I712074
sequence annotation: BBa_K1497007
sequence annotation: BBa_I712074
sequence: https://synbiohub.org/public/igem/BBa_K1497017_sequence/1

BBa_K1497007
type: Component
persistent identity: component2398904
display id: component2398904
version: 1
top level: BBa_K1497017
access: public
definition: BBa_K1497007

BBa_I712074
type: Component
persistent identity: component2398886
display id: component2398886
version: 1
top level: BBa_K1497017
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K1497007
type: SequenceAnnotation
persistent identity: annotation2398904
display id: annotation2398904
version: 1
top level: BBa_K1497017
location: https://synbiohub.org/public/igem/BBa_K1497017/annotation2398904/range2398904/1
component: BBa_K1497007

https://synbiohub.org/public/igem/BBa_K1497017/annotation2398904/range2398904/1
type: Range
persistent identity: range2398904
display id: range2398904
version: 1
direction: direction/reverse
top level: BBa_K1497017
start: 55
end: 5357
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2398886
display id: annotation2398886
version: 1
top level: BBa_K1497017
location: https://synbiohub.org/public/igem/BBa_K1497017/annotation2398886/range2398886/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1497017/annotation2398886/range2398886/1
type: Range
persistent identity: range2398886
display id: range2398886
version: 1
direction: direction/reverse
top level: BBa_K1497017
start: 1
end: 46
orientation: inline

BBa_K801072
type: ComponentDefinition
persistent identity: BBa K801072
display id: BBa_K801072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3,7-dimethylxanthine N-methyltransferase CaDXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB084125 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: CaDXMT1
sequence annotation: Consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_K801072_sequence/1

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198187
display id: annotation2198187
version: 1
top level: BBa_K801072
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198187/range2198187/1
type: Range
persistent identity: range2198187
display id: range2198187
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1159
end: 1188
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198588
display id: annotation2198588
version: 1
top level: BBa_K801072
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198588/range2198588/1
type: Range
persistent identity: range2198588
display id: range2198588
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1189
end: 1194
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198600
display id: annotation2198600
version: 1
top level: BBa_K801072
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2198600/range2198600/1
type: Range
persistent identity: range2198600
display id: range2198600
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 9
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197894
display id: annotation2197894
version: 1
top level: BBa_K801072
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197894/range2197894/1
type: Range
persistent identity: range2197894
display id: range2197894
version: 1
direction: direction/forward
top level: BBa_K801072
start: 7
end: 1158
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197893
display id: annotation2197893
version: 1
top level: BBa_K801072
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1

https://synbiohub.org/public/igem/BBa_K801072/annotation2197893/range2197893/1
type: Range
persistent identity: range2197893
display id: range2197893
version: 1
direction: direction/forward
top level: BBa_K801072
start: 1
end: 6
orientation: inline

BBa_M36006
type: ComponentDefinition
persistent identity: BBa M36006
display id: BBa_M36006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glucose1phosphate adenylyltransferase
creator: Jesse Palmer, Elliot Lui
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details.
mutable notes: This mutation results in greater glycogen production compared to WT.
mutable provenance: The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->T
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_M36006_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation2119202
display id: annotation2119202
version: 1
top level: BBa_M36006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119202/range2119202/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119202/range2119202/1
type: Range
persistent identity: range2119202
display id: range2119202
version: 1
direction: direction/forward
top level: BBa_M36006
start: 199
end: 199
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119203
display id: annotation2119203
version: 1
top level: BBa_M36006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119203/range2119203/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119203/range2119203/1
type: Range
persistent identity: range2119203
display id: range2119203
version: 1
direction: direction/forward
top level: BBa_M36006
start: 1294
end: 1296
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119064
display id: annotation2119064
version: 1
top level: BBa_M36006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36006/annotation2119064/range2119064/1

https://synbiohub.org/public/igem/BBa_M36006/annotation2119064/range2119064/1
type: Range
persistent identity: range2119064
display id: range2119064
version: 1
direction: direction/forward
top level: BBa_M36006
start: 1
end: 3
orientation: inline

BBa_K1497010
type: ComponentDefinition
persistent identity: BBa K1497010
display id: BBa_K1497010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dihydroflavonol 4-reductase (DFR)
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497010_sequence/1

BBa_K1172306
type: ComponentDefinition
persistent identity: BBa K1172306
display id: BBa_K1172306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboflavin synthesis gene cluster from s. oneidensis under control of a strong Anderson promoter
creator: Thorben Meyer
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172303
component: BBa_K608002
sequence annotation: BBa_K608002
sequence annotation: BBa_K1172303
sequence: https://synbiohub.org/public/igem/BBa_K1172306_sequence/1

BBa_K1172303
type: Component
persistent identity: component2355312
display id: component2355312
version: 1
top level: BBa_K1172306
access: public
definition: rib-synth

BBa_K608002
type: Component
persistent identity: component2355311
display id: component2355311
version: 1
top level: BBa_K1172306
access: public
definition: https://synbiohub.org/public/igem/BBa_K608002/1

BBa_K608002
type: SequenceAnnotation
persistent identity: annotation2355311
display id: annotation2355311
version: 1
top level: BBa_K1172306
location: https://synbiohub.org/public/igem/BBa_K1172306/annotation2355311/range2355311/1
component: BBa_K608002

https://synbiohub.org/public/igem/BBa_K1172306/annotation2355311/range2355311/1
type: Range
persistent identity: range2355311
display id: range2355311
version: 1
direction: direction/reverse
top level: BBa_K1172306
start: 1
end: 55
orientation: inline

BBa_K1172303
type: SequenceAnnotation
persistent identity: annotation2355312
display id: annotation2355312
version: 1
top level: BBa_K1172306
location: https://synbiohub.org/public/igem/BBa_K1172306/annotation2355312/range2355312/1
component: BBa_K1172303

https://synbiohub.org/public/igem/BBa_K1172306/annotation2355312/range2355312/1
type: Range
persistent identity: range2355312
display id: range2355312
version: 1
direction: direction/reverse
top level: BBa_K1172306
start: 62
end: 3658
orientation: inline

BBa_K1331003
type: ComponentDefinition
persistent identity: BBa K1331003
display id: BBa_K1331003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
mutable notes: It includes both the complete coding sequence of RhlI and part of noncoding sequence between RhlR and RhlI gene.
mutable provenance: It comes from the genomic sequence of P.aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlI
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331003_sequence/1

RhlI
type: SequenceAnnotation
persistent identity: annotation2427973
display id: annotation2427973
version: 1
top level: BBa_K1331003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1
type: Range
persistent identity: range2427973
display id: range2427973
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 114
end: 719
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427981
display id: annotation2427981
version: 1
top level: BBa_K1331003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1
type: Range
persistent identity: range2427981
display id: range2427981
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 1
end: 113
orientation: inline

BBa_K808013
type: ComponentDefinition
persistent identity: BBa K808013
display id: BBa_K808013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA3: Catalyzes together with tphA2 TPA to DCD
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K808013_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2181847
display id: annotation2181847
version: 1
top level: BBa_K808013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808013/annotation2181847/range2181847/1

https://synbiohub.org/public/igem/BBa_K808013/annotation2181847/range2181847/1
type: Range
persistent identity: range2181847
display id: range2181847
version: 1
direction: direction/forward
top level: BBa_K808013
start: 1
end: 465
orientation: inline

BBa_K801092
type: ComponentDefinition
persistent identity: BBa K801092
display id: BBa_K801092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate - coenzym A ligase (4CL) coding region from ''Arabidopsis thaliana'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: 4CL
sequence: https://synbiohub.org/public/igem/BBa_K801092_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2196267
display id: annotation2196267
version: 1
top level: BBa_K801092
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2196267/range2196267/1
type: Range
persistent identity: range2196267
display id: range2196267
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1
end: 6
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202114
display id: annotation2202114
version: 1
top level: BBa_K801092
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2202114/range2202114/1
type: Range
persistent identity: range2202114
display id: range2202114
version: 1
direction: direction/forward
top level: BBa_K801092
start: 1690
end: 1692
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201481
display id: annotation2201481
version: 1
top level: BBa_K801092
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201481/range2201481/1
type: Range
persistent identity: range2201481
display id: range2201481
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 9
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2201482
display id: annotation2201482
version: 1
top level: BBa_K801092
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1

https://synbiohub.org/public/igem/BBa_K801092/annotation2201482/range2201482/1
type: Range
persistent identity: range2201482
display id: range2201482
version: 1
direction: direction/forward
top level: BBa_K801092
start: 7
end: 1689
orientation: inline

DGAT
type: ComponentDefinition
persistent identity: BBa K836001
display id: BBa_K836001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein DGAT (Diacylglycerol O-acyltransferase) which have dual activity, it can act as a wax ester synthase or as a diacylglycerol acyltransferase. In the latter case, its function consists in mediate the final step in TGAs synthesis using a fatty acyl-CoA and a DGA. This enzyme is vital in the storage and synthesis of lipids. Based on Uniprot information, this enzyme is more likely to act as a wax ester synthase with (C12-C18)-CoA whereas with (C20)-CoA it acts as a DGAT.
mutable notes: Two transcriptional terminators (two biobricks BBa_B1006) were set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (as in the rest of biobricks, the prefix and suffix are not present in the sequence).
mutable provenance: The amino sequence of this part was taken from Uniprot (wax-dgaT accesion Q8GGG1; http://www.uniprot.org/uniprot/Q8GGG1) and then codon optimization for R. opacus (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DGAT
sequence: https://synbiohub.org/public/igem/BBa_K836001_sequence/1

DGAT
type: SequenceAnnotation
persistent identity: annotation2185260
display id: annotation2185260
version: 1
top level: DGAT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836001/annotation2185260/range2185260/1

https://synbiohub.org/public/igem/BBa_K836001/annotation2185260/range2185260/1
type: Range
persistent identity: range2185260
display id: range2185260
version: 1
direction: direction/forward
top level: DGAT
start: 1
end: 1380
orientation: inline

BBa_K1497005
type: ComponentDefinition
persistent identity: BBa K1497005
display id: BBa_K1497005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is able to generate Naringenin from 4-courmaroyl-CoA, if put under a promotor. This device is the second composite part for the assembly of our Naringenin Operon (K1497007). Part 1: K1497004: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034. Part 2: K1497003: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Petunia and Gerbara
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497003
component: BBa_K1497004
sequence annotation: BBa_K1497004
sequence annotation: BBa_K1497003
sequence: https://synbiohub.org/public/igem/BBa_K1497005_sequence/1

BBa_K1497003
type: Component
persistent identity: component2416912
display id: component2416912
version: 1
top level: BBa_K1497005
access: public
definition: BBa_K1497003

BBa_K1497004
type: Component
persistent identity: component2416916
display id: component2416916
version: 1
top level: BBa_K1497005
access: public
definition: BBa_K1497004

BBa_K1497004
type: SequenceAnnotation
persistent identity: annotation2416916
display id: annotation2416916
version: 1
top level: BBa_K1497005
location: https://synbiohub.org/public/igem/BBa_K1497005/annotation2416916/range2416916/1
component: BBa_K1497004

https://synbiohub.org/public/igem/BBa_K1497005/annotation2416916/range2416916/1
type: Range
persistent identity: range2416916
display id: range2416916
version: 1
direction: direction/reverse
top level: BBa_K1497005
start: 753
end: 1967
orientation: inline

BBa_K1497003
type: SequenceAnnotation
persistent identity: annotation2416912
display id: annotation2416912
version: 1
top level: BBa_K1497005
location: https://synbiohub.org/public/igem/BBa_K1497005/annotation2416912/range2416912/1
component: BBa_K1497003

https://synbiohub.org/public/igem/BBa_K1497005/annotation2416912/range2416912/1
type: Range
persistent identity: range2416912
display id: range2416912
version: 1
direction: direction/reverse
top level: BBa_K1497005
start: 1
end: 744
orientation: inline

Biosurfact
type: ComponentDefinition
persistent identity: BBa K653000
display id: BBa_K653000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Re-designing "The Biosurfactator" The first Central American BioBrick
creator: Team Panama 2011
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (RhlAB). This is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid, which is a biomolecule with surfactant properties, but the natural RhlAB gene has illegal restriction sites that make it incompatible with the Assembly Standard Protocol 10, specifically the Pstl1 restriction sites. The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year???s iGEM project we failed in producing the rhamnolipid compound into our E. coli based-factory, because our last year???s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part BBa_K424018 (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone. As scientist and iGEMers, we have the responsibility to deliver a functional BioBrick, thus according to iGEM???s competition new slogan, ???Quality not Quantity???, we have embraced the challenge of re-designing and re-building the same BioBrick part from last year in order to comply this new standard based on quality. How to use it as a BioBrick? : We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
mutable notes: The RhlAB gene complex has shown illegal restriction sites according to the assembly standard protocol 10. We faced this problem by applying the mutagenesis protocol that consist in doing silent mutations that change the nucleotides of our rhlAB gene that are cut by the Pstl restriction enzyme. This will give us a standardized coding sequence of the rhlAB gene compatible with the Assembly Standard protocol 10 and a new BioBrick part for the Registry.
mutable provenance: Pseudomonas aeruginosa from INDICASAT lab. and the genomic sequence of RhlAB was reviwed in genBank (ID 878955 and 878954) http://www.ncbi.nlm.nih.gov/nuccore/L28170.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Silent Mutation
sequence annotation: Rhamnosyltransferase B
sequence annotation: Rhamnosyltranferase A
sequence annotation: Primer
sequence annotation: BBa_ K653000 RhlAB Panama
sequence annotation: Rhamnosyltransferase gene complex 1
sequence: https://synbiohub.org/public/igem/BBa_K653000_sequence/1

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142965
display id: annotation2142965
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1
type: Range
persistent identity: range2142965
display id: range2142965
version: 1
direction: direction/forward
top level: Biosurfact
start: 754
end: 759
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142968
display id: annotation2142968
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1
type: Range
persistent identity: range2142968
display id: range2142968
version: 1
direction: direction/forward
top level: Biosurfact
start: 1891
end: 1896
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142967
display id: annotation2142967
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1
type: Range
persistent identity: range2142967
display id: range2142967
version: 1
direction: direction/forward
top level: Biosurfact
start: 1777
end: 1782
orientation: inline

Rhamnosyltransferase B
type: SequenceAnnotation
persistent identity: annotation2142966
display id: annotation2142966
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1
type: Range
persistent identity: range2142966
display id: range2142966
version: 1
direction: direction/forward
top level: Biosurfact
start: 988
end: 2268
orientation: inline

Rhamnosyltranferase A
type: SequenceAnnotation
persistent identity: annotation2142964
display id: annotation2142964
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1
type: Range
persistent identity: range2142964
display id: range2142964
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 922
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142962
display id: annotation2142962
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1
type: Range
persistent identity: range2142962
display id: range2142962
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 19
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142969
display id: annotation2142969
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1
type: Range
persistent identity: range2142969
display id: range2142969
version: 1
direction: direction/reverse
top level: Biosurfact
start: 2296
end: 2314
orientation: inline

BBa_ K653000 RhlAB Panama
type: SequenceAnnotation
persistent identity: annotation2142961
display id: annotation2142961
version: 1
top level: Biosurfact
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1
type: Range
persistent identity: range2142961
display id: range2142961
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 2314
orientation: inline

Rhamnosyltransferase gene complex 1
type: SequenceAnnotation
persistent identity: annotation2142963
display id: annotation2142963
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1
type: Range
persistent identity: range2142963
display id: range2142963
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 2234
orientation: inline

RcsB
type: ComponentDefinition
persistent identity: BBa K200000
display id: BBa_K200000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colanic acid global regulator -> RcsB
creator: James Field
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _298_
m user id: 0
owner id: 5277
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: RcsB is a receiver protein in a two component phosphorelay system. Up-regulation of RcsB has been shown to induce colanic acid production. This is via the activation of the ugd operon which is required for capsule synthesis.
mutable notes: RcsB also upregulates ftsZ which increases the rate of cell division.
mutable provenance: NCBI Reference Sequence: NC_000913.2 >gi|49175990:2314199-2314849
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RcsB gene
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K200000_sequence/1

RcsB gene
type: SequenceAnnotation
persistent identity: annotation2041558
display id: annotation2041558
version: 1
top level: RcsB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041558/range2041558/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041558/range2041558/1
type: Range
persistent identity: range2041558
display id: range2041558
version: 1
direction: direction/forward
top level: RcsB
start: 1
end: 648
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2041559
display id: annotation2041559
version: 1
top level: RcsB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041559/range2041559/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041559/range2041559/1
type: Range
persistent identity: range2041559
display id: range2041559
version: 1
direction: direction/forward
top level: RcsB
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2041560
display id: annotation2041560
version: 1
top level: RcsB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041560/range2041560/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041560/range2041560/1
type: Range
persistent identity: range2041560
display id: range2041560
version: 1
direction: direction/forward
top level: RcsB
start: 649
end: 653
orientation: inline

BBa_M36014
type: ComponentDefinition
persistent identity: BBa M36014
display id: BBa_M36014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (alt2)
creator: Jesse Palmer, Elliot Lui
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase) and glgA gene (coding for glycogen synthase).
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
mutable provenance: Composite of genomic sequences coding for proteins involved in e. coli glycogen biosynthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36006
component: BBa_M36724
component: BBa_M36007
component: BBa_M36009
component: BBa_M36010
sequence annotation: BBa_M36010
sequence annotation: BBa_M36724
sequence annotation: BBa_M36007
sequence annotation: BBa_M36009
sequence annotation: BBa_M36006
sequence: https://synbiohub.org/public/igem/BBa_M36014_sequence/1

BBa_M36006
type: Component
persistent identity: component2118545
display id: component2118545
version: 1
top level: BBa_M36014
access: public
definition: BBa_M36006

BBa_M36724
type: Component
persistent identity: component2118546
display id: component2118546
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36007
type: Component
persistent identity: component2118547
display id: component2118547
version: 1
top level: BBa_M36014
access: public
definition: BBa_M36007

BBa_M36009
type: Component
persistent identity: component2118544
display id: component2118544
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36010
type: Component
persistent identity: component2118548
display id: component2118548
version: 1
top level: BBa_M36014
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118548
display id: annotation2118548
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118548/range2118548/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36014/annotation2118548/range2118548/1
type: Range
persistent identity: range2118548
display id: range2118548
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 2863
end: 2944
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118546
display id: annotation2118546
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118546/range2118546/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36014/annotation2118546/range2118546/1
type: Range
persistent identity: range2118546
display id: range2118546
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1344
end: 1428
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2118547
display id: annotation2118547
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118547/range2118547/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36014/annotation2118547/range2118547/1
type: Range
persistent identity: range2118547
display id: range2118547
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1429
end: 2862
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118544
display id: annotation2118544
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118544/range2118544/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36014/annotation2118544/range2118544/1
type: Range
persistent identity: range2118544
display id: range2118544
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 1
end: 47
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118545
display id: annotation2118545
version: 1
top level: BBa_M36014
location: https://synbiohub.org/public/igem/BBa_M36014/annotation2118545/range2118545/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36014/annotation2118545/range2118545/1
type: Range
persistent identity: range2118545
display id: range2118545
version: 1
direction: direction/reverse
top level: BBa_M36014
start: 48
end: 1343
orientation: inline

BBa_K1462030
type: ComponentDefinition
persistent identity: BBa K1462030
display id: BBa_K1462030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hbd
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462030_sequence/1

BBa_K2042012
type: ComponentDefinition
persistent identity: BBa K2042012
display id: BBa_K2042012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + PhaC_sp. MBEL 6-19
creator: Delphine Dessaux
created: 2016-09-02T11:00:00Z
modified: 2016-10-10T09:14:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31381
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS + PhaC_v4
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K2042003
component: BBa_K2042002
component: BBa_K2042016
sequence annotation: BBa_K2042002
sequence annotation: BBa_K2042003
sequence annotation: BBa_K2042016
sequence: https://synbiohub.org/public/igem/BBa_K2042012_sequence/1

BBa_K2042003
type: Component
persistent identity: component2482798
display id: component2482798
version: 1
top level: BBa_K2042012
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042003/1

BBa_K2042002
type: Component
persistent identity: component2482800
display id: component2482800
version: 1
top level: BBa_K2042012
access: public
definition: BBa_K2042002

BBa_K2042016
type: Component
persistent identity: component2482799
display id: component2482799
version: 1
top level: BBa_K2042012
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042016/1

BBa_K2042002
type: SequenceAnnotation
persistent identity: annotation2482800
display id: annotation2482800
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482800/range2482800/1
component: BBa_K2042002

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482800/range2482800/1
type: Range
persistent identity: range2482800
display id: range2482800
version: 1
direction: direction/reverse
top level: BBa_K2042012
start: 14
end: 1693
orientation: inline

BBa_K2042003
type: SequenceAnnotation
persistent identity: annotation2482798
display id: annotation2482798
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482798/range2482798/1
component: BBa_K2042003

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482798/range2482798/1
type: Range
persistent identity: range2482798
display id: range2482798
version: 1
direction: direction/reverse
top level: BBa_K2042012
start: 1
end: 7
orientation: inline

BBa_K2042016
type: SequenceAnnotation
persistent identity: annotation2482799
display id: annotation2482799
version: 1
top level: BBa_K2042012
location: https://synbiohub.org/public/igem/BBa_K2042012/annotation2482799/range2482799/1
component: BBa_K2042016

https://synbiohub.org/public/igem/BBa_K2042012/annotation2482799/range2482799/1
type: Range
persistent identity: range2482799
display id: range2482799
version: 1
direction: direction/reverse
top level: BBa_K2042012
start: 8
end: 13
orientation: inline

BBa_K1315004
type: ComponentDefinition
persistent identity: BBa K1315004
display id: BBa_K1315004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ydeH (dgcZ)
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:49Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Diguanylate cyclases produce cyclic diGMP (c-di-GMP), a secondary messenger. Diguanylate Cyclase (DgcZ), also called YdeH, is present in Escherichia coli and the stimulus that controls it is Zinc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector. YdeH however, is toxic to E. coli cells.
mutable provenance: The source of this part is Escherichia coli MC40100 genomic DNA which was provided by the Tracy Palmer lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YdeH
sequence: https://synbiohub.org/public/igem/BBa_K1315004_sequence/1

YdeH
type: SequenceAnnotation
persistent identity: annotation2386526
display id: annotation2386526
version: 1
top level: BBa_K1315004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1

https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1
type: Range
persistent identity: range2386526
display id: range2386526
version: 1
direction: direction/forward
top level: BBa_K1315004
start: 1
end: 894
orientation: inline

BBa_K1152000
type: ComponentDefinition
persistent identity: BBa K1152000
display id: BBa_K1152000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TycA_deltaCom Module from Tyrocidine
creator: Tania Christiansen
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
group u list: _1464_
m user id: 0
owner id: 16899
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus Parabrevis. It is the first module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine. This specific module Tyca_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of the A- (adenylation), PCP- (peptide carrier protein) and the E- (epimerization) domain. After Tyc A follows the TycB gene with three modules. This bio brick can be used for the design of different NRPS. It was used in the synthetic Tripeptide I of our project.
mutable notes: This is just the coding sequence. We sequenced with VR and VF2 primer, not mutations were found.
mutable provenance: Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: Coding Sequence TycA_deltaCom
sequence: https://synbiohub.org/public/igem/BBa_K1152000_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2367791
display id: annotation2367791
version: 1
top level: BBa_K1152000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1152000/annotation2367791/range2367791/1

https://synbiohub.org/public/igem/BBa_K1152000/annotation2367791/range2367791/1
type: Range
persistent identity: range2367791
display id: range2367791
version: 1
direction: direction/forward
top level: BBa_K1152000
start: 1
end: 3
orientation: inline

Coding Sequence TycA_deltaCom
type: SequenceAnnotation
persistent identity: annotation2367790
display id: annotation2367790
version: 1
top level: BBa_K1152000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1152000/annotation2367790/range2367790/1

https://synbiohub.org/public/igem/BBa_K1152000/annotation2367790/range2367790/1
type: Range
persistent identity: range2367790
display id: range2367790
version: 1
direction: direction/forward
top level: BBa_K1152000
start: 1
end: 3186
orientation: inline

BBa_K1497001
type: ComponentDefinition
persistent identity: BBa K1497001
display id: BBa_K1497001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CHS - Chalcone Synthase from Gerbera
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.
mutable notes: no
mutable provenance: Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone. We used the CHS and verified the function in our Naringenin Operon (K1497007).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497001_sequence/1

BBa_K801071
type: ComponentDefinition
persistent identity: BBa K801071
display id: BBa_K801071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7-methylxanthine N-methyltransferase CaMXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 7-methylxanthine N-methyltransferase CaMXMT1 from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: Synthesized by IDT, Yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CaMXMT1
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence annotation: Strep Tag
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K801071_sequence/1

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197709
display id: annotation2197709
version: 1
top level: BBa_K801071
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197709/range2197709/1
type: Range
persistent identity: range2197709
display id: range2197709
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 1140
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198586
display id: annotation2198586
version: 1
top level: BBa_K801071
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198586/range2198586/1
type: Range
persistent identity: range2198586
display id: range2198586
version: 1
direction: direction/forward
top level: BBa_K801071
start: 7
end: 9
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197876
display id: annotation2197876
version: 1
top level: BBa_K801071
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2197876/range2197876/1
type: Range
persistent identity: range2197876
display id: range2197876
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1
end: 6
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198148
display id: annotation2198148
version: 1
top level: BBa_K801071
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198148/range2198148/1
type: Range
persistent identity: range2198148
display id: range2198148
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1141
end: 1170
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198587
display id: annotation2198587
version: 1
top level: BBa_K801071
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1

https://synbiohub.org/public/igem/BBa_K801071/annotation2198587/range2198587/1
type: Range
persistent identity: range2198587
display id: range2198587
version: 1
direction: direction/forward
top level: BBa_K801071
start: 1171
end: 1176
orientation: inline

ninabGen
type: ComponentDefinition
persistent identity: BBa K343005
display id: BBa_K343005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-carotene 15
creator: Christian Kurtzhals
created: 2010-09-06T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
mutable notes: A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
mutable provenance: [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K343001
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343005_sequence/1

BBa_R0040
type: Component
persistent identity: component2079700
display id: component2079700
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K343001
type: Component
persistent identity: component2079710
display id: component2079710
version: 1
top level: ninabGen
access: public
definition: ninaB

BBa_B0034
type: Component
persistent identity: component2079706
display id: component2079706
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2079700
display id: annotation2079700
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
component: BBa_R0040

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type: Range
persistent identity: range2079700
display id: range2079700
version: 1
direction: direction/forward
top level: ninabGen
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079706
display id: annotation2079706
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
type: Range
persistent identity: range2079706
display id: range2079706
version: 1
direction: direction/reverse
top level: ninabGen
start: 63
end: 74
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2079710
display id: annotation2079710
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
type: Range
persistent identity: range2079710
display id: range2079710
version: 1
direction: direction/reverse
top level: ninabGen
start: 81
end: 1943
orientation: inline

BBa_K332022
type: ComponentDefinition
persistent identity: BBa K332022
display id: BBa_K332022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux + RBS + mRFP + double terminator
creator: Chin-Han Huang
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _452_
m user id: 0
owner id: 7483
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With Plux gene,it can detect the concentration of AHL-LuxR protein complex and initiate the following gene Sequence and Features
mutable notes: to detect cell's population
mutable provenance: all from IGEM's gene bank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0062
component: BBa_J61048
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_J61048
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K332022_sequence/1

BBa_B0034
type: Component
persistent identity: component2250415
display id: component2250415
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component2250409
display id: component2250409
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_J61048
type: Component
persistent identity: component2250419
display id: component2250419
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_E1010
type: Component
persistent identity: component2250418
display id: component2250418
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250418
display id: annotation2250418
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250418/range2250418/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K332022/annotation2250418/range2250418/1
type: Range
persistent identity: range2250418
display id: range2250418
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 82
end: 787
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250419
display id: annotation2250419
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250419/range2250419/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332022/annotation2250419/range2250419/1
type: Range
persistent identity: range2250419
display id: range2250419
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 796
end: 908
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2250409
display id: annotation2250409
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250409/range2250409/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K332022/annotation2250409/range2250409/1
type: Range
persistent identity: range2250409
display id: range2250409
version: 1
direction: direction/forward
top level: BBa_K332022
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2250415
display id: annotation2250415
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250415/range2250415/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332022/annotation2250415/range2250415/1
type: Range
persistent identity: range2250415
display id: range2250415
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 64
end: 75
orientation: inline

BBa_K1497016
type: ComponentDefinition
persistent identity: BBa K1497016
display id: BBa_K1497016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive naringenin producing operon
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23100
component: BBa_K1497007
sequence annotation: BBa_J23100
sequence annotation: BBa_K1497007
sequence: https://synbiohub.org/public/igem/BBa_K1497016_sequence/1

BBa_J23100
type: Component
persistent identity: component2398911
display id: component2398911
version: 1
top level: BBa_K1497016
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1497007
type: Component
persistent identity: component2398929
display id: component2398929
version: 1
top level: BBa_K1497016
access: public
definition: BBa_K1497007

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2398911
display id: annotation2398911
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398911/range2398911/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1497016/annotation2398911/range2398911/1
type: Range
persistent identity: range2398911
display id: range2398911
version: 1
direction: direction/reverse
top level: BBa_K1497016
start: 1
end: 35
orientation: inline

BBa_K1497007
type: SequenceAnnotation
persistent identity: annotation2398929
display id: annotation2398929
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398929/range2398929/1
component: BBa_K1497007

https://synbiohub.org/public/igem/BBa_K1497016/annotation2398929/range2398929/1
type: Range
persistent identity: range2398929
display id: range2398929
version: 1
direction: direction/reverse
top level: BBa_K1497016
start: 44
end: 5346
orientation: inline

BBa_K801096
type: ComponentDefinition
persistent identity: BBa K801096
display id: BBa_K801096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aromatic prenyltransferase (APT) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Aromatic prenyltransferase (APT) coding region from ''Humulus lupulus'', yeast codon optimized.
mutable notes: -
mutable provenance: Synthesized by Geneart, yeast codon optimized http://www.ncbi.nlm.nih.gov/nuccore/AB543053
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: APT
sequence: https://synbiohub.org/public/igem/BBa_K801096_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202205
display id: annotation2202205
version: 1
top level: BBa_K801096
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801096/annotation2202205/range2202205/1

https://synbiohub.org/public/igem/BBa_K801096/annotation2202205/range2202205/1
type: Range
persistent identity: range2202205
display id: range2202205
version: 1
direction: direction/forward
top level: BBa_K801096
start: 1
end: 3
orientation: inline

APT
type: SequenceAnnotation
persistent identity: annotation2202207
display id: annotation2202207
version: 1
top level: BBa_K801096
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801096/annotation2202207/range2202207/1

https://synbiohub.org/public/igem/BBa_K801096/annotation2202207/range2202207/1
type: Range
persistent identity: range2202207
display id: range2202207
version: 1
direction: direction/forward
top level: BBa_K801096
start: 1
end: 1233
orientation: inline

BBa_K1497009
type: ComponentDefinition
persistent identity: BBa K1497009
display id: BBa_K1497009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497009_sequence/1

BBa_M36007
type: ComponentDefinition
persistent identity: BBa M36007
display id: BBa_M36007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glycogen synthase
creator: Jesse Palmer, Elliot Lui
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details.
mutable provenance: Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M36007_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2119212
display id: annotation2119212
version: 1
top level: BBa_M36007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36007/annotation2119212/range2119212/1

https://synbiohub.org/public/igem/BBa_M36007/annotation2119212/range2119212/1
type: Range
persistent identity: range2119212
display id: range2119212
version: 1
direction: direction/forward
top level: BBa_M36007
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2119213
display id: annotation2119213
version: 1
top level: BBa_M36007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36007/annotation2119213/range2119213/1

https://synbiohub.org/public/igem/BBa_M36007/annotation2119213/range2119213/1
type: Range
persistent identity: range2119213
display id: range2119213
version: 1
direction: direction/forward
top level: BBa_M36007
start: 1432
end: 1434
orientation: inline

BBa_K1331001
type: ComponentDefinition
persistent identity: BBa K1331001
display id: BBa_K1331001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa.
mutable notes: A mutation has been done to remove the PstI restriction site contained in the coding sequence.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: rhlA
sequence: https://synbiohub.org/public/igem/BBa_K1331001_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427978
display id: annotation2427978
version: 1
top level: BBa_K1331001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1
type: Range
persistent identity: range2427978
display id: range2427978
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 889
end: 947
orientation: inline

rhlA
type: SequenceAnnotation
persistent identity: annotation2386609
display id: annotation2386609
version: 1
top level: BBa_K1331001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1
type: Range
persistent identity: range2386609
display id: range2386609
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 1
end: 888
orientation: inline

BBa_K1497018
type: ComponentDefinition
persistent identity: BBa K1497018
display id: BBa_K1497018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-F3H-B0034-DFR
creator: Sascha Hein, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: s
mutable notes: s
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497012
component: BBa_K1497013
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497013
sequence: https://synbiohub.org/public/igem/BBa_K1497018_sequence/1

BBa_K1497012
type: Component
persistent identity: component2398934
display id: component2398934
version: 1
top level: BBa_K1497018
access: public
definition: BBa_K1497012

BBa_K1497013
type: Component
persistent identity: component2398938
display id: component2398938
version: 1
top level: BBa_K1497018
access: public
definition: BBa_K1497013

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2398934
display id: annotation2398934
version: 1
top level: BBa_K1497018
location: https://synbiohub.org/public/igem/BBa_K1497018/annotation2398934/range2398934/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497018/annotation2398934/range2398934/1
type: Range
persistent identity: range2398934
display id: range2398934
version: 1
direction: direction/reverse
top level: BBa_K1497018
start: 1
end: 1125
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2398938
display id: annotation2398938
version: 1
top level: BBa_K1497018
location: https://synbiohub.org/public/igem/BBa_K1497018/annotation2398938/range2398938/1
component: BBa_K1497013

https://synbiohub.org/public/igem/BBa_K1497018/annotation2398938/range2398938/1
type: Range
persistent identity: range2398938
display id: range2398938
version: 1
direction: direction/reverse
top level: BBa_K1497018
start: 1134
end: 2213
orientation: inline

BBa_K801075
type: ComponentDefinition
persistent identity: BBa K801075
display id: BBa_K801075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaDXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Yeast expression cassette for CaDXMT1 (BBa_K801072) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012)
mutable notes: -
mutable provenance: Composite part of BBa_K801010, BBa_K801072 and BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence annotation: ADH1 Terminator
sequence annotation: CaDXMT1
sequence annotation: Cassette
sequence annotation: Stop codon
sequence annotation: Tef2 promoter
sequence annotation: Strep-Tag
sequence: https://synbiohub.org/public/igem/BBa_K801075_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2201347
display id: annotation2201347
version: 1
top level: BBa_K801075
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2201347/range2201347/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2201347/range2201347/1
type: Range
persistent identity: range2201347
display id: range2201347
version: 1
direction: direction/forward
top level: BBa_K801075
start: 663
end: 665
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199506
display id: annotation2199506
version: 1
top level: BBa_K801075
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199506/range2199506/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199506/range2199506/1
type: Range
persistent identity: range2199506
display id: range2199506
version: 1
direction: direction/forward
top level: BBa_K801075
start: 657
end: 662
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197243
display id: annotation2197243
version: 1
top level: BBa_K801075
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197243/range2197243/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197243/range2197243/1
type: Range
persistent identity: range2197243
display id: range2197243
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1859
end: 2208
orientation: inline

CaDXMT1
type: SequenceAnnotation
persistent identity: annotation2197242
display id: annotation2197242
version: 1
top level: BBa_K801075
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197242/range2197242/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197242/range2197242/1
type: Range
persistent identity: range2197242
display id: range2197242
version: 1
direction: direction/forward
top level: BBa_K801075
start: 663
end: 1814
orientation: inline

Cassette
type: SequenceAnnotation
persistent identity: annotation2189809
display id: annotation2189809
version: 1
top level: BBa_K801075
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2189809/range2189809/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2189809/range2189809/1
type: Range
persistent identity: range2189809
display id: range2189809
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1
end: 2208
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199508
display id: annotation2199508
version: 1
top level: BBa_K801075
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199508/range2199508/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199508/range2199508/1
type: Range
persistent identity: range2199508
display id: range2199508
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1845
end: 1850
orientation: inline

Tef2 promoter
type: SequenceAnnotation
persistent identity: annotation2197241
display id: annotation2197241
version: 1
top level: BBa_K801075
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2197241/range2197241/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2197241/range2197241/1
type: Range
persistent identity: range2197241
display id: range2197241
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1
end: 648
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199509
display id: annotation2199509
version: 1
top level: BBa_K801075
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801075/annotation2199509/range2199509/1

https://synbiohub.org/public/igem/BBa_K801075/annotation2199509/range2199509/1
type: Range
persistent identity: range2199509
display id: range2199509
version: 1
direction: direction/forward
top level: BBa_K801075
start: 1815
end: 1844
orientation: inline

BBa_K801081
type: ComponentDefinition
persistent identity: BBa K801081
display id: BBa_K801081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prepro-Thaumatin expression cassette for yeast
creator: Alois Br??uer
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part can be used to constitutively express Prepro-Thaumatin in ''S. cerevisiae'' under the control of the TEF1-promoter and the TEF1-terminator.
mutable notes: -
mutable provenance: Based on part BBa_K801080 (Prepro-Thaumatin).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K801080
component: BBa_K319003
component: BBa_K801011
sequence annotation: BBa_K801011
sequence annotation: BBa_K319003
sequence annotation: BBa_K801080
sequence: https://synbiohub.org/public/igem/BBa_K801081_sequence/1

BBa_K801080
type: Component
persistent identity: component2373054
display id: component2373054
version: 1
top level: BBa_K801081
access: public
definition: BBa_K801080

BBa_K319003
type: Component
persistent identity: component2373050
display id: component2373050
version: 1
top level: BBa_K801081
access: public
definition: https://synbiohub.org/public/igem/BBa_K319003/1

BBa_K801011
type: Component
persistent identity: component2373056
display id: component2373056
version: 1
top level: BBa_K801081
access: public
definition: https://synbiohub.org/public/igem/BBa_K801011/1

BBa_K801011
type: SequenceAnnotation
persistent identity: annotation2373056
display id: annotation2373056
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373056/range2373056/1
component: BBa_K801011

https://synbiohub.org/public/igem/BBa_K801081/annotation2373056/range2373056/1
type: Range
persistent identity: range2373056
display id: range2373056
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 1173
end: 1679
orientation: inline

BBa_K319003
type: SequenceAnnotation
persistent identity: annotation2373050
display id: annotation2373050
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373050/range2373050/1
component: BBa_K319003

https://synbiohub.org/public/igem/BBa_K801081/annotation2373050/range2373050/1
type: Range
persistent identity: range2373050
display id: range2373050
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 1
end: 450
orientation: inline

BBa_K801080
type: SequenceAnnotation
persistent identity: annotation2373054
display id: annotation2373054
version: 1
top level: BBa_K801081
location: https://synbiohub.org/public/igem/BBa_K801081/annotation2373054/range2373054/1
component: BBa_K801080

https://synbiohub.org/public/igem/BBa_K801081/annotation2373054/range2373054/1
type: Range
persistent identity: range2373054
display id: range2373054
version: 1
direction: direction/reverse
top level: BBa_K801081
start: 457
end: 1164
orientation: inline

BBa_K1497003
type: ComponentDefinition
persistent identity: BBa K1497003
display id: BBa_K1497003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-10T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Petunia
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1497000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1497000
sequence: https://synbiohub.org/public/igem/BBa_K1497003_sequence/1

BBa_K1497000
type: Component
persistent identity: component2416908
display id: component2416908
version: 1
top level: BBa_K1497003
access: public
definition: BBa_K1497000

BBa_B0034
type: Component
persistent identity: component2416907
display id: component2416907
version: 1
top level: BBa_K1497003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2416907
display id: annotation2416907
version: 1
top level: BBa_K1497003
location: https://synbiohub.org/public/igem/BBa_K1497003/annotation2416907/range2416907/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497003/annotation2416907/range2416907/1
type: Range
persistent identity: range2416907
display id: range2416907
version: 1
direction: direction/reverse
top level: BBa_K1497003
start: 1
end: 12
orientation: inline

BBa_K1497000
type: SequenceAnnotation
persistent identity: annotation2416908
display id: annotation2416908
version: 1
top level: BBa_K1497003
location: https://synbiohub.org/public/igem/BBa_K1497003/annotation2416908/range2416908/1
component: BBa_K1497000

https://synbiohub.org/public/igem/BBa_K1497003/annotation2416908/range2416908/1
type: Range
persistent identity: range2416908
display id: range2416908
version: 1
direction: direction/reverse
top level: BBa_K1497003
start: 19
end: 744
orientation: inline

frr
type: ComponentDefinition
persistent identity: BBa K1668002
display id: BBa_K1668002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: frr (from Streptomyces avermitilis, increasing avermectin production)
creator: Xintian Xu
created: 2015-09-07T11:00:00Z
modified: 2015-09-11T06:20:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2086_
m user id: 24510
owner id: 24510
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Frr gene encodes the ribosome recycling factor (RRF), which is involved in the release of ribosomes from the translational post-termination complex for a new round of initiation. In comparison to the wild-type strain, avermectin yield was increased significantly by 3- to 3.7-fold in transformants 31267(pFRR-1139) and 31267(pFRRerm-1139), both of which contained multiple frr copies, compared with that in the control strains. The avermectin productivity of each culture was quantitatively measured by HPLC analysis. Research indicated that frr overexpression promoted cell growth as well as the expression of ave genes (including pathway-specific positive regulatory gene aveR for avermectin biosynthesis and ave structural genes), leading in turn to avermectin overproduction. Different from S-adenosylmethionine synthetase gene (metK), frr gene revealed a ??????copy number effect??????: multiple copies of frr had a greater promoting effect on avermectin production than a single copy in each of the three transformed S. avermitilis strains.
mutable notes: We used seamless assembly.
mutable provenance: The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop coden
sequence annotation: start coden
sequence: https://synbiohub.org/public/igem/BBa_K1668002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2456727
display id: annotation2456727
version: 1
top level: frr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2456727/range2456727/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2456727/range2456727/1
type: Range
persistent identity: range2456727
display id: range2456727
version: 1
direction: direction/forward
top level: frr
start: 1
end: 384
orientation: inline

stop coden
type: SequenceAnnotation
persistent identity: annotation2449088
display id: annotation2449088
version: 1
top level: frr
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2449088/range2449088/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2449088/range2449088/1
type: Range
persistent identity: range2449088
display id: range2449088
version: 1
direction: direction/forward
top level: frr
start: 379
end: 384
orientation: inline

start coden
type: SequenceAnnotation
persistent identity: annotation2449087
display id: annotation2449087
version: 1
top level: frr
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2449087/range2449087/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2449087/range2449087/1
type: Range
persistent identity: range2449087
display id: range2449087
version: 1
direction: direction/forward
top level: frr
start: 1
end: 3
orientation: inline

BBa_M36013
type: ComponentDefinition
persistent identity: BBa M36013
display id: BBa_M36013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (alt1)
creator: Jesse Palmer, Elliot Lui
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase) and glgS gene (predicted glycogen synthesis protein). This part is greatly reduced in size from the ideal glycogen synthesis actuator by the removal of glgA.
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
mutable provenance: Composite of genomic sequences coding for proteins involved in e. coli glycogen biosynthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36008
component: BBa_M36724
component: BBa_M36006
component: BBa_M36010
component: BBa_M36009
sequence annotation: BBa_M36009
sequence annotation: BBa_M36010
sequence annotation: BBa_M36006
sequence annotation: BBa_M36008
sequence annotation: BBa_M36724
sequence: https://synbiohub.org/public/igem/BBa_M36013_sequence/1

BBa_M36008
type: Component
persistent identity: component2118542
display id: component2118542
version: 1
top level: BBa_M36013
access: public
definition: BBa_M36008

BBa_M36724
type: Component
persistent identity: component2118541
display id: component2118541
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36006
type: Component
persistent identity: component2118540
display id: component2118540
version: 1
top level: BBa_M36013
access: public
definition: BBa_M36006

BBa_M36010
type: Component
persistent identity: component2118543
display id: component2118543
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36009
type: Component
persistent identity: component2118539
display id: component2118539
version: 1
top level: BBa_M36013
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118539
display id: annotation2118539
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118539/range2118539/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36013/annotation2118539/range2118539/1
type: Range
persistent identity: range2118539
display id: range2118539
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1
end: 47
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118543
display id: annotation2118543
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118543/range2118543/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36013/annotation2118543/range2118543/1
type: Range
persistent identity: range2118543
display id: range2118543
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1630
end: 1711
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118540
display id: annotation2118540
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118540/range2118540/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36013/annotation2118540/range2118540/1
type: Range
persistent identity: range2118540
display id: range2118540
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 48
end: 1343
orientation: inline

BBa_M36008
type: SequenceAnnotation
persistent identity: annotation2118542
display id: annotation2118542
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118542/range2118542/1
component: BBa_M36008

https://synbiohub.org/public/igem/BBa_M36013/annotation2118542/range2118542/1
type: Range
persistent identity: range2118542
display id: range2118542
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1429
end: 1629
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118541
display id: annotation2118541
version: 1
top level: BBa_M36013
location: https://synbiohub.org/public/igem/BBa_M36013/annotation2118541/range2118541/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36013/annotation2118541/range2118541/1
type: Range
persistent identity: range2118541
display id: range2118541
version: 1
direction: direction/reverse
top level: BBa_M36013
start: 1344
end: 1428
orientation: inline

BBa_M36002
type: ComponentDefinition
persistent identity: BBa M36002
display id: BBa_M36002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gluc1phos adenylyltrans (with 5'UTR & term)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details.
mutable notes: This mutation results in greater glycogen production compared to WT.
mutable provenance: The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation in the glgC gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_M36006
component: BBa_M36010
component: BBa_M36009
sequence annotation: BBa_M36006
sequence annotation: BBa_M36010
sequence annotation: BBa_M36009
sequence: https://synbiohub.org/public/igem/BBa_M36002_sequence/1

BBa_M36006
type: Component
persistent identity: component2117835
display id: component2117835
version: 1
top level: BBa_M36002
access: public
definition: BBa_M36006

BBa_M36010
type: Component
persistent identity: component2117836
display id: component2117836
version: 1
top level: BBa_M36002
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36009
type: Component
persistent identity: component2117834
display id: component2117834
version: 1
top level: BBa_M36002
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2117835
display id: annotation2117835
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117835/range2117835/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36002/annotation2117835/range2117835/1
type: Range
persistent identity: range2117835
display id: range2117835
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 48
end: 1343
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2117836
display id: annotation2117836
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117836/range2117836/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36002/annotation2117836/range2117836/1
type: Range
persistent identity: range2117836
display id: range2117836
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 1344
end: 1425
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2117834
display id: annotation2117834
version: 1
top level: BBa_M36002
location: https://synbiohub.org/public/igem/BBa_M36002/annotation2117834/range2117834/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36002/annotation2117834/range2117834/1
type: Range
persistent identity: range2117834
display id: range2117834
version: 1
direction: direction/reverse
top level: BBa_M36002
start: 1
end: 47
orientation: inline

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet4
sequence annotation: Beta sheet3
sequence annotation: Beta sheet2
sequence annotation: GCT into GCC
sequence annotation: Linker
sequence annotation: CsgA-His1
sequence annotation: Beta sheet 1
sequence annotation: His tag
sequence annotation: CsgG signal
sequence annotation: Beta sheet5
sequence annotation: RBS Paris 2009
sequence annotation: Sec signal
sequence annotation: BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

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type: Range
persistent identity: range2426219
display id: range2426219
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start: 92
end: 577
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Beta sheet 1
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display id: annotation2426223
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top level: BBa_K1404007
role: SO:0000110
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location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
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direction: direction/forward
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start: 218
end: 286
orientation: inline

His tag
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display id: annotation2426230
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top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
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direction: direction/forward
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start: 557
end: 574
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CsgG signal
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role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

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type: Range
persistent identity: range2426222
display id: range2426222
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start: 152
end: 217
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
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start: 488
end: 544
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
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persistent identity: range2426220
display id: range2426220
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start: 79
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Sec signal
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persistent identity: annotation2426221
display id: annotation2426221
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top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

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type: Range
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display id: range2426221
version: 1
direction: direction/forward
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start: 92
end: 151
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
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start: 1
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orientation: inline

BBa_K1172304
type: ComponentDefinition
persistent identity: BBa K1172304
display id: BBa_K1172304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS
creator: Thorben Meyer
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172303
component: BBa_K525998
sequence annotation: BBa_K1172303
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K1172304_sequence/1

BBa_K1172303
type: Component
persistent identity: component2355198
display id: component2355198
version: 1
top level: BBa_K1172304
access: public
definition: rib-synth

BBa_K525998
type: Component
persistent identity: component2355197
display id: component2355197
version: 1
top level: BBa_K1172304
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K1172303
type: SequenceAnnotation
persistent identity: annotation2355198
display id: annotation2355198
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1172304/annotation2355198/range2355198/1
component: BBa_K1172303

https://synbiohub.org/public/igem/BBa_K1172304/annotation2355198/range2355198/1
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BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2355197
display id: annotation2355197
version: 1
top level: BBa_K1172304
location: https://synbiohub.org/public/igem/BBa_K1172304/annotation2355197/range2355197/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1172304/annotation2355197/range2355197/1
type: Range
persistent identity: range2355197
display id: range2355197
version: 1
direction: direction/reverse
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start: 1
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BBa_K801070
type: ComponentDefinition
persistent identity: BBa K801070
display id: BBa_K801070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xanthosine methyltransferase CaXMT1-strep
creator: Roman Prechtl
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: xanthosine methyltransferase CaXMT1-strep from ''Coffea arabica'' followed by a strep-tag for purification and/or detection via western blot.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/AB048793 synthesized by IDT, yeast codon optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: CaXMT1
sequence annotation: Start codon
sequence annotation: Consensus sequence
sequence annotation: Strep Tag
sequence: https://synbiohub.org/public/igem/BBa_K801070_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2198401
display id: annotation2198401
version: 1
top level: BBa_K801070
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198401/range2198401/1
type: Range
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display id: range2198401
version: 1
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start: 1153
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orientation: inline

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2198398
display id: annotation2198398
version: 1
top level: BBa_K801070
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198398/range2198398/1
type: Range
persistent identity: range2198398
display id: range2198398
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 1122
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2198399
display id: annotation2198399
version: 1
top level: BBa_K801070
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198399/range2198399/1
type: Range
persistent identity: range2198399
display id: range2198399
version: 1
direction: direction/forward
top level: BBa_K801070
start: 7
end: 9
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2198397
display id: annotation2198397
version: 1
top level: BBa_K801070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198397/range2198397/1
type: Range
persistent identity: range2198397
display id: range2198397
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1
end: 6
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2198400
display id: annotation2198400
version: 1
top level: BBa_K801070
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1

https://synbiohub.org/public/igem/BBa_K801070/annotation2198400/range2198400/1
type: Range
persistent identity: range2198400
display id: range2198400
version: 1
direction: direction/forward
top level: BBa_K801070
start: 1123
end: 1152
orientation: inline

BBa_K1497013
type: ComponentDefinition
persistent identity: BBa K1497013
display id: BBa_K1497013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-DFR
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1497010
component: BBa_B0034
sequence annotation: BBa_K1497010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497013_sequence/1

BBa_K1497010
type: Component
persistent identity: component2398837
display id: component2398837
version: 1
top level: BBa_K1497013
access: public
definition: BBa_K1497010

BBa_B0034
type: Component
persistent identity: component2398836
display id: component2398836
version: 1
top level: BBa_K1497013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497010
type: SequenceAnnotation
persistent identity: annotation2398837
display id: annotation2398837
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
component: BBa_K1497010

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type: Range
persistent identity: range2398837
display id: range2398837
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 19
end: 1080
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398836
display id: annotation2398836
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
type: Range
persistent identity: range2398836
display id: range2398836
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 1
end: 12
orientation: inline

tphB
type: ComponentDefinition
persistent identity: BBa K808010
display id: BBa_K808010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphB: reaction from DCD to protocatechuate
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K808010_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2181625
display id: annotation2181625
version: 1
top level: tphB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1

https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1
type: Range
persistent identity: range2181625
display id: range2181625
version: 1
direction: direction/forward
top level: tphB
start: 1
end: 948
orientation: inline

BBa_M36012
type: ComponentDefinition
persistent identity: BBa M36012
display id: BBa_M36012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glycogen Synthesis Actuator (ideal)
creator: Elliott Lui and Jesse Palmer
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9642
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Expresses the glgC, glgA, and glgS proteins/enzymes usually not expressed in e. coli.
mutable notes: Using different RBSs so that secondary structures and self pairing don't occur.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36006
component: BBa_M36007
component: BBa_M36724
component: BBa_M36009
component: BBa_M36001
component: BBa_M36010
component: BBa_M36008
sequence annotation: BBa_M36001
sequence annotation: BBa_M36008
sequence annotation: BBa_M36009
sequence annotation: BBa_M36010
sequence annotation: BBa_M36724
sequence annotation: BBa_M36006
sequence annotation: BBa_M36007
sequence: https://synbiohub.org/public/igem/BBa_M36012_sequence/1

BBa_M36006
type: Component
persistent identity: component2118437
display id: component2118437
version: 1
top level: BBa_M36012
access: public
definition: BBa_M36006

BBa_M36007
type: Component
persistent identity: component2118439
display id: component2118439
version: 1
top level: BBa_M36012
access: public
definition: BBa_M36007

BBa_M36724
type: Component
persistent identity: component2118440
display id: component2118440
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36724/1

BBa_M36009
type: Component
persistent identity: component2118436
display id: component2118436
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36001
type: Component
persistent identity: component2118438
display id: component2118438
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36001/1

BBa_M36010
type: Component
persistent identity: component2118442
display id: component2118442
version: 1
top level: BBa_M36012
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36008
type: Component
persistent identity: component2118441
display id: component2118441
version: 1
top level: BBa_M36012
access: public
definition: BBa_M36008

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2118438
display id: annotation2118438
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118438/range2118438/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36012/annotation2118438/range2118438/1
type: Range
persistent identity: range2118438
display id: range2118438
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1344
end: 1390
orientation: inline

BBa_M36008
type: SequenceAnnotation
persistent identity: annotation2118441
display id: annotation2118441
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118441/range2118441/1
component: BBa_M36008

https://synbiohub.org/public/igem/BBa_M36012/annotation2118441/range2118441/1
type: Range
persistent identity: range2118441
display id: range2118441
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 2910
end: 3110
orientation: inline

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2118436
display id: annotation2118436
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118436/range2118436/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36012/annotation2118436/range2118436/1
type: Range
persistent identity: range2118436
display id: range2118436
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1
end: 47
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118442
display id: annotation2118442
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118442/range2118442/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36012/annotation2118442/range2118442/1
type: Range
persistent identity: range2118442
display id: range2118442
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 3111
end: 3192
orientation: inline

BBa_M36724
type: SequenceAnnotation
persistent identity: annotation2118440
display id: annotation2118440
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118440/range2118440/1
component: BBa_M36724

https://synbiohub.org/public/igem/BBa_M36012/annotation2118440/range2118440/1
type: Range
persistent identity: range2118440
display id: range2118440
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 2825
end: 2909
orientation: inline

BBa_M36006
type: SequenceAnnotation
persistent identity: annotation2118437
display id: annotation2118437
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118437/range2118437/1
component: BBa_M36006

https://synbiohub.org/public/igem/BBa_M36012/annotation2118437/range2118437/1
type: Range
persistent identity: range2118437
display id: range2118437
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 48
end: 1343
orientation: inline

BBa_M36007
type: SequenceAnnotation
persistent identity: annotation2118439
display id: annotation2118439
version: 1
top level: BBa_M36012
location: https://synbiohub.org/public/igem/BBa_M36012/annotation2118439/range2118439/1
component: BBa_M36007

https://synbiohub.org/public/igem/BBa_M36012/annotation2118439/range2118439/1
type: Range
persistent identity: range2118439
display id: range2118439
version: 1
direction: direction/reverse
top level: BBa_M36012
start: 1391
end: 2824
orientation: inline

BBa_K1067006
type: ComponentDefinition
persistent identity: BBa K1067006
display id: BBa_K1067006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Denitrification operon from Pseudomonas aeruginosa
creator: Kristian Davidsen, Helen Cook and Jakob Berg Jespersen
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: fff
mutable notes: fff
mutable provenance: fff
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cytochrome c-551
sequence annotation: Uroporphyrin-III c-methyltransferase
sequence annotation: Heme d1 biosynthesis protein
sequence annotation: Nitrite reductase
sequence annotation: cytochrome c precursor
sequence annotation: c-type cytochrome
sequence: https://synbiohub.org/public/igem/BBa_K1067006_sequence/1

cytochrome c-551
type: SequenceAnnotation
persistent identity: annotation2366467
display id: annotation2366467
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366467/range2366467/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366467/range2366467/1
type: Range
persistent identity: range2366467
display id: range2366467
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 1758
end: 2072
orientation: inline

Uroporphyrin-III c-methyltransferase
type: SequenceAnnotation
persistent identity: annotation2366477
display id: annotation2366477
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366477/range2366477/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366477/range2366477/1
type: Range
persistent identity: range2366477
display id: range2366477
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 6679
end: 7518
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366475
display id: annotation2366475
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366475/range2366475/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366475/range2366475/1
type: Range
persistent identity: range2366475
display id: range2366475
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 4061
end: 4585
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366472
display id: annotation2366472
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366472/range2366472/1

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type: Range
persistent identity: range2366472
display id: range2366472
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 2425
end: 3603
orientation: inline

Nitrite reductase
type: SequenceAnnotation
persistent identity: annotation2366465
display id: annotation2366465
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366465/range2366465/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366465/range2366465/1
type: Range
persistent identity: range2366465
display id: range2366465
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 1
end: 1707
orientation: inline

cytochrome c precursor
type: SequenceAnnotation
persistent identity: annotation2366470
display id: annotation2366470
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366470/range2366470/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366470/range2366470/1
type: Range
persistent identity: range2366470
display id: range2366470
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 2069
end: 2428
orientation: inline

c-type cytochrome
type: SequenceAnnotation
persistent identity: annotation2366478
display id: annotation2366478
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366478/range2366478/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366478/range2366478/1
type: Range
persistent identity: range2366478
display id: range2366478
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 7509
end: 8990
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366476
display id: annotation2366476
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366476/range2366476/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366476/range2366476/1
type: Range
persistent identity: range2366476
display id: range2366476
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 5505
end: 6668
orientation: inline

BBa_K323122
type: ComponentDefinition
persistent identity: BBa K323122
display id: BBa_K323122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0011 crtEBIYZ
creator: Tina Ilc
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _443_
m user id: 0
owner id: 7571
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K274210
component: BBa_I742158
sequence annotation: BBa_K274210
sequence annotation: BBa_I742158
sequence: https://synbiohub.org/public/igem/BBa_K323122_sequence/1

BBa_K274210
type: Component
persistent identity: component2093499
display id: component2093499
version: 1
top level: BBa_K323122
access: public
definition: https://synbiohub.org/public/igem/BBa_K274210/1

BBa_I742158
type: Component
persistent identity: component2093503
display id: component2093503
version: 1
top level: BBa_K323122
access: public
definition: https://synbiohub.org/public/igem/BBa_I742158/1

BBa_K274210
type: SequenceAnnotation
persistent identity: annotation2093499
display id: annotation2093499
version: 1
top level: BBa_K323122
location: https://synbiohub.org/public/igem/BBa_K323122/annotation2093499/range2093499/1
component: BBa_K274210

https://synbiohub.org/public/igem/BBa_K323122/annotation2093499/range2093499/1
type: Range
persistent identity: range2093499
display id: range2093499
version: 1
direction: direction/reverse
top level: BBa_K323122
start: 1
end: 4618
orientation: inline

BBa_I742158
type: SequenceAnnotation
persistent identity: annotation2093503
display id: annotation2093503
version: 1
top level: BBa_K323122
location: https://synbiohub.org/public/igem/BBa_K323122/annotation2093503/range2093503/1
component: BBa_I742158

https://synbiohub.org/public/igem/BBa_K323122/annotation2093503/range2093503/1
type: Range
persistent identity: range2093503
display id: range2093503
version: 1
direction: direction/reverse
top level: BBa_K323122
start: 4627
end: 5173
orientation: inline

BBa_J212099
type: ComponentDefinition
persistent identity: BBa J212099
display id: BBa_J212099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: test
creator: Shuangxi Ren
created: 2012-02-03T12:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: true
dominant: false
experience: experience/None
group u list: _921_
m user id: 0
owner id: 11311
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: this is a test.
mutable notes: rfc10
mutable provenance: home-made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J212099_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2170086
display id: annotation2170086
version: 1
top level: BBa_J212099
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J212099/annotation2170086/range2170086/1

https://synbiohub.org/public/igem/BBa_J212099/annotation2170086/range2170086/1
type: Range
persistent identity: range2170086
display id: range2170086
version: 1
direction: direction/forward
top level: BBa_J212099
start: 1
end: 5
orientation: inline

BBa_K552003
type: ComponentDefinition
persistent identity: BBa K552003
display id: BBa_K552003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtI Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This DNA sequence codes for the enzyme CrtI, also known as phytoene desaturase. CrtI converts pytoene to lycopene which is an important step in the carotenoid biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CrtI
sequence annotation: Stop Codon
sequence annotation: ADH1 Terminator
sequence annotation: PGK Promoter
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K552003_sequence/1

CrtI
type: SequenceAnnotation
persistent identity: annotation2145132
display id: annotation2145132
version: 1
top level: BBa_K552003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145132/range2145132/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145132/range2145132/1
type: Range
persistent identity: range2145132
display id: range2145132
version: 1
direction: direction/forward
top level: BBa_K552003
start: 381
end: 2129
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145133
display id: annotation2145133
version: 1
top level: BBa_K552003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145133/range2145133/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145133/range2145133/1
type: Range
persistent identity: range2145133
display id: range2145133
version: 1
direction: direction/forward
top level: BBa_K552003
start: 2127
end: 2129
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145134
display id: annotation2145134
version: 1
top level: BBa_K552003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145134/range2145134/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145134/range2145134/1
type: Range
persistent identity: range2145134
display id: range2145134
version: 1
direction: direction/forward
top level: BBa_K552003
start: 2130
end: 2258
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145122
display id: annotation2145122
version: 1
top level: BBa_K552003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145122/range2145122/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145122/range2145122/1
type: Range
persistent identity: range2145122
display id: range2145122
version: 1
direction: direction/forward
top level: BBa_K552003
start: 1
end: 380
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145131
display id: annotation2145131
version: 1
top level: BBa_K552003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552003/annotation2145131/range2145131/1

https://synbiohub.org/public/igem/BBa_K552003/annotation2145131/range2145131/1
type: Range
persistent identity: range2145131
display id: range2145131
version: 1
direction: direction/forward
top level: BBa_K552003
start: 381
end: 383
orientation: inline

BBa_K2042002
type: ComponentDefinition
persistent identity: BBa K2042002
display id: BBa_K2042002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas sp. MBEL 6-19 PHA synthase 1
creator: Maria Bargu??s i Ribera
created: 2016-09-01T11:00:00Z
modified: 2016-10-15T02:38:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: S477G
sequence annotation: S325T
sequence annotation: Pst1 removed
sequence annotation: Start
sequence annotation: Stop
sequence annotation: E130D
sequence annotation: Q481K
sequence annotation: PhaC
sequence: https://synbiohub.org/public/igem/BBa_K2042002_sequence/1

S477G
type: SequenceAnnotation
persistent identity: annotation2512501
display id: annotation2512501
version: 1
top level: BBa_K2042002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512501/range2512501/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512501/range2512501/1
type: Range
persistent identity: range2512501
display id: range2512501
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1429
end: 1429
orientation: inline

S325T
type: SequenceAnnotation
persistent identity: annotation2512503
display id: annotation2512503
version: 1
top level: BBa_K2042002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512503/range2512503/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512503/range2512503/1
type: Range
persistent identity: range2512503
display id: range2512503
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 974
end: 974
orientation: inline

Pst1 removed
type: SequenceAnnotation
persistent identity: annotation2512502
display id: annotation2512502
version: 1
top level: BBa_K2042002
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512502/range2512502/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512502/range2512502/1
type: Range
persistent identity: range2512502
display id: range2512502
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1416
end: 1416
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512476
display id: annotation2512476
version: 1
top level: BBa_K2042002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512476/range2512476/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512476/range2512476/1
type: Range
persistent identity: range2512476
display id: range2512476
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1
end: 3
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2512477
display id: annotation2512477
version: 1
top level: BBa_K2042002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512477/range2512477/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512477/range2512477/1
type: Range
persistent identity: range2512477
display id: range2512477
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1678
end: 1680
orientation: inline

Pst1 removed
type: SequenceAnnotation
persistent identity: annotation2512499
display id: annotation2512499
version: 1
top level: BBa_K2042002
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512499/range2512499/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512499/range2512499/1
type: Range
persistent identity: range2512499
display id: range2512499
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 974
end: 974
orientation: inline

E130D
type: SequenceAnnotation
persistent identity: annotation2512498
display id: annotation2512498
version: 1
top level: BBa_K2042002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512498/range2512498/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512498/range2512498/1
type: Range
persistent identity: range2512498
display id: range2512498
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 390
end: 390
orientation: inline

Q481K
type: SequenceAnnotation
persistent identity: annotation2512500
display id: annotation2512500
version: 1
top level: BBa_K2042002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512500/range2512500/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512500/range2512500/1
type: Range
persistent identity: range2512500
display id: range2512500
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1441
end: 1441
orientation: inline

PhaC
type: SequenceAnnotation
persistent identity: annotation2512474
display id: annotation2512474
version: 1
top level: BBa_K2042002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2042002/annotation2512474/range2512474/1

https://synbiohub.org/public/igem/BBa_K2042002/annotation2512474/range2512474/1
type: Range
persistent identity: range2512474
display id: range2512474
version: 1
direction: direction/forward
top level: BBa_K2042002
start: 1
end: 1680
orientation: inline

BBa_K1497004
type: ComponentDefinition
persistent identity: BBa K1497004
display id: BBa_K1497004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Gerbera
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497004_sequence/1

BBa_B0034
type: Component
persistent identity: component2398582
display id: component2398582
version: 1
top level: BBa_K1497004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398582
display id: annotation2398582
version: 1
top level: BBa_K1497004
location: https://synbiohub.org/public/igem/BBa_K1497004/annotation2398582/range2398582/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497004/annotation2398582/range2398582/1
type: Range
persistent identity: range2398582
display id: range2398582
version: 1
direction: direction/reverse
top level: BBa_K1497004
start: 1
end: 12
orientation: inline

BBa_K1497006
type: ComponentDefinition
persistent identity: BBa K1497006
display id: BBa_K1497006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-4CL-B0034-TAL
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite part is the fusion device of the Biobrick parts K1033001 by Uppsala 2013 and K1033000 by. It is able to produce 4-coumaroyl-CoA from tyrosine, if put under a promotor. This device is the first composite part for the assembly of our Naringenin Operon (K1497007). We used the previously announced Biobricks to assemble this part and verified the function in our Naringenin Operon (K1497007).
mutable notes: no
mutable provenance: w
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1033001
component: BBa_K1033000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033001
sequence annotation: BBa_K1033000
sequence: https://synbiohub.org/public/igem/BBa_K1497006_sequence/1

BBa_K1033001
type: Component
persistent identity: component2398658
display id: component2398658
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033001/1

BBa_K1033000
type: Component
persistent identity: component2398663
display id: component2398663
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033000/1

BBa_B0034
type: Component
persistent identity: component2398655
display id: component2398655
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2398660
display id: component2398660
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398660
display id: annotation2398660
version: 1
top level: BBa_K1497006
location: https://synbiohub.org/public/igem/BBa_K1497006/annotation2398660/range2398660/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497006/annotation2398660/range2398660/1
type: Range
persistent identity: range2398660
display id: range2398660
version: 1
direction: direction/reverse
top level: BBa_K1497006
start: 1737
end: 1748
orientation: inline

BBa_K1033001
type: SequenceAnnotation
persistent identity: annotation2398658
display id: annotation2398658
version: 1
top level: BBa_K1497006
location: https://synbiohub.org/public/igem/BBa_K1497006/annotation2398658/range2398658/1
component: BBa_K1033001

https://synbiohub.org/public/igem/BBa_K1497006/annotation2398658/range2398658/1
type: Range
persistent identity: range2398658
display id: range2398658
version: 1
direction: direction/reverse
top level: BBa_K1497006
start: 21
end: 1728
orientation: inline

BBa_K1033000
type: SequenceAnnotation
persistent identity: annotation2398663
display id: annotation2398663
version: 1
top level: BBa_K1497006
location: https://synbiohub.org/public/igem/BBa_K1497006/annotation2398663/range2398663/1
component: BBa_K1033000

https://synbiohub.org/public/igem/BBa_K1497006/annotation2398663/range2398663/1
type: Range
persistent identity: range2398663
display id: range2398663
version: 1
direction: direction/reverse
top level: BBa_K1497006
start: 1757
end: 3352
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398655
display id: annotation2398655
version: 1
top level: BBa_K1497006
location: https://synbiohub.org/public/igem/BBa_K1497006/annotation2398655/range2398655/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497006/annotation2398655/range2398655/1
type: Range
persistent identity: range2398655
display id: range2398655
version: 1
direction: direction/reverse
top level: BBa_K1497006
start: 1
end: 12
orientation: inline

BBa_K801091
type: ComponentDefinition
persistent identity: BBa K801091
display id: BBa_K801091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phenylalanine ammonia lyase (PAL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the enzyme phenylalanine ammonia lyase (PAL) from ''Rhodosporidium toruloides''
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nuccore/AX366866 PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PAL
sequence annotation: Start codon
sequence annotation: Mutation
sequence annotation: Phe instead of Ser
sequence annotation: Deletion of Cys
sequence: https://synbiohub.org/public/igem/BBa_K801091_sequence/1

PAL
type: SequenceAnnotation
persistent identity: annotation2202130
display id: annotation2202130
version: 1
top level: BBa_K801091
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202130/range2202130/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202130/range2202130/1
type: Range
persistent identity: range2202130
display id: range2202130
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1
end: 2148
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202128
display id: annotation2202128
version: 1
top level: BBa_K801091
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202128/range2202128/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202128/range2202128/1
type: Range
persistent identity: range2202128
display id: range2202128
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1
end: 3
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2202129
display id: annotation2202129
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202129/range2202129/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202129/range2202129/1
type: Range
persistent identity: range2202129
display id: range2202129
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1356
end: 1356
orientation: inline

Phe instead of Ser
type: SequenceAnnotation
persistent identity: annotation2202137
display id: annotation2202137
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202137/range2202137/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202137/range2202137/1
type: Range
persistent identity: range2202137
display id: range2202137
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1364
end: 1364
orientation: inline

Deletion of Cys
type: SequenceAnnotation
persistent identity: annotation2202133
display id: annotation2202133
version: 1
top level: BBa_K801091
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801091/annotation2202133/range2202133/1

https://synbiohub.org/public/igem/BBa_K801091/annotation2202133/range2202133/1
type: Range
persistent identity: range2202133
display id: range2202133
version: 1
direction: direction/forward
top level: BBa_K801091
start: 1358
end: 1360
orientation: inline

BBa_K1122676
type: ComponentDefinition
persistent identity: BBa K1122676
display id: BBa_K1122676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible pdc and adhB
creator: Aleksandra Lewicka, Jan Lyczakowski
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This parts enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) using IPTG induction. Those enzymes are involved in conversion of pyruvate to ethanol via an acetaldehyde intermediate. The pdc and adhB sequences come from ''Zymomonas mobilis'' however, they are codon optimised for ''E. coli''.
mutable notes: Presence of LacZ alpha peptide upstream of the ORFs coding for the enzymes enables blue- white screening.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J33207
component: BBa_K173003
sequence annotation: BBa_K173003
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K1122676_sequence/1

BBa_J33207
type: Component
persistent identity: component2333624
display id: component2333624
version: 1
top level: BBa_K1122676
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K173003
type: Component
persistent identity: component2333639
display id: component2333639
version: 1
top level: BBa_K1122676
access: public
definition: https://synbiohub.org/public/igem/BBa_K173003/1

BBa_K173003
type: SequenceAnnotation
persistent identity: annotation2333639
display id: annotation2333639
version: 1
top level: BBa_K1122676
location: https://synbiohub.org/public/igem/BBa_K1122676/annotation2333639/range2333639/1
component: BBa_K173003

https://synbiohub.org/public/igem/BBa_K1122676/annotation2333639/range2333639/1
type: Range
persistent identity: range2333639
display id: range2333639
version: 1
direction: direction/reverse
top level: BBa_K1122676
start: 609
end: 3660
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2333624
display id: annotation2333624
version: 1
top level: BBa_K1122676
location: https://synbiohub.org/public/igem/BBa_K1122676/annotation2333624/range2333624/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K1122676/annotation2333624/range2333624/1
type: Range
persistent identity: range2333624
display id: range2333624
version: 1
direction: direction/reverse
top level: BBa_K1122676
start: 1
end: 600
orientation: inline

nosZ
type: ComponentDefinition
persistent identity: BBa K1356006
display id: BBa_K1356006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrous oxide reductase (nosZ) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: L
mutable notes: Mutation- fail!
mutable provenance: P.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356006_sequence/1

BBa_K801098
type: ComponentDefinition
persistent identity: BBa K801098
display id: BBa_K801098
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: O-methyltransferase 1 (OMT1) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: O-methyltransferase 1 (OMT1) coding region from ''Humulus lupulus''
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nucleotide/167613934/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: OMT
sequence: https://synbiohub.org/public/igem/BBa_K801098_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202173
display id: annotation2202173
version: 1
top level: BBa_K801098
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801098/annotation2202173/range2202173/1

https://synbiohub.org/public/igem/BBa_K801098/annotation2202173/range2202173/1
type: Range
persistent identity: range2202173
display id: range2202173
version: 1
direction: direction/forward
top level: BBa_K801098
start: 1
end: 3
orientation: inline

OMT
type: SequenceAnnotation
persistent identity: annotation2202174
display id: annotation2202174
version: 1
top level: BBa_K801098
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801098/annotation2202174/range2202174/1

https://synbiohub.org/public/igem/BBa_K801098/annotation2202174/range2202174/1
type: Range
persistent identity: range2202174
display id: range2202174
version: 1
direction: direction/forward
top level: BBa_K801098
start: 1
end: 1056
orientation: inline

BBa_K2042001
type: ComponentDefinition
persistent identity: BBa K2042001
display id: BBa_K2042001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas resinovorans PHA synthase 1
creator: Toky RATOVOMANANA
created: 2016-09-01T11:00:00Z
modified: 2016-10-10T09:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding sequence for a Polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence is codon optimized for pseudomonas putida it includes four mutations which were found to increase yields of PLA (Yang et al.) site-directed mutagenesis, the mutation leads to production of high molecular weights of P(3HB-co-LA); site-directed mutagenesis, the type II PHA synthase 1 is engineered to accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA) by site-directed mutagenesis of four sites, i.e. E130, S325, S477, and Q481 We note that unlike BioBrick BBa K1211002 Yale 2013, one of the mutation is not on the Serine 477 on Serine 475. which is able to do the reaction: lactylCoA + PLA(n) = PLA(n+1).
mutable notes: We used this sequence as second enzyme in order to produce PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere
mutable provenance: This sequence was synthetized by IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: PhaC
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K2042001_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2512256
display id: annotation2512256
version: 1
top level: BBa_K2042001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2042001/annotation2512256/range2512256/1

https://synbiohub.org/public/igem/BBa_K2042001/annotation2512256/range2512256/1
type: Range
persistent identity: range2512256
display id: range2512256
version: 1
direction: direction/forward
top level: BBa_K2042001
start: 1678
end: 1680
orientation: inline

PhaC
type: SequenceAnnotation
persistent identity: annotation2512238
display id: annotation2512238
version: 1
top level: BBa_K2042001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2042001/annotation2512238/range2512238/1

https://synbiohub.org/public/igem/BBa_K2042001/annotation2512238/range2512238/1
type: Range
persistent identity: range2512238
display id: range2512238
version: 1
direction: direction/forward
top level: BBa_K2042001
start: 1
end: 1680
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512239
display id: annotation2512239
version: 1
top level: BBa_K2042001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2042001/annotation2512239/range2512239/1

https://synbiohub.org/public/igem/BBa_K2042001/annotation2512239/range2512239/1
type: Range
persistent identity: range2512239
display id: range2512239
version: 1
direction: direction/forward
top level: BBa_K2042001
start: 1
end: 3
orientation: inline

pET fusion
type: ComponentDefinition
persistent identity: BBa K1122673
display id: BBa_K1122673
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ethanol production module
creator: Aleksandra Lewicka, Jan Lyczakowski
created: 2013-08-15T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for the fusion of Pyruvate decarboxylase and Alcohol dehydrogenase from ''Zymomonas mobilis''. Fused enzymes increase ethanol yields and enable its faster production.
mutable notes: The part is based on the BBa_K173003 - the enzymatic components of this BioBrick were fused using MABEL technology. The fusion removed start codon in adhB, stop codon in pdc and the RBS inbetween.
mutable provenance: ''Z. mobilis'' gDNA codon optimised for ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122673_sequence/1

BBa_K1462020
type: ComponentDefinition
persistent identity: BBa K1462020
display id: BBa_K1462020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erg10
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462020_sequence/1

BBa_K1497000
type: ComponentDefinition
persistent identity: BBa K1497000
display id: BBa_K1497000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chalcone Isomerase (CHI) from Petunia
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. We used the CHI and verified the function in our Naringenin Operon (K1497007).
mutable notes: Original Sequence from Petunia
mutable provenance: Petunia Hybrida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497000_sequence/1

nirS
type: ComponentDefinition
persistent identity: BBa K1356003
display id: BBa_K1356003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrite Reductase (nirS) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for the nitrite reductase (nirS) that converts nitrite (NO2-) into nitric oxide (NO). It is cloned into the pSB1C3
mutable notes: Nothing
mutable provenance: P. aeruginosa PAO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356003_sequence/1

BBa_K1479016
type: ComponentDefinition
persistent identity: BBa K1479016
display id: BBa_K1479016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: fsadfasdfasdfasfdasdfa
mutable notes: sadfasdfadfsdfa
mutable provenance: adsfsdfasdfasdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 1
sequence annotation: 4

1
type: SequenceAnnotation
persistent identity: annotation2418316
display id: annotation2418316
version: 1
top level: BBa_K1479016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1
type: Range
persistent identity: range2418316
display id: range2418316
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 2
end: 3
orientation: inline

4
type: SequenceAnnotation
persistent identity: annotation2418317
display id: annotation2418317
version: 1
top level: BBa_K1479016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1
type: Range
persistent identity: range2418317
display id: range2418317
version: 1
direction: direction/forward
top level: BBa_K1479016
start: 5
end: 6
orientation: inline

norC
type: ComponentDefinition
persistent identity: BBa K1356004
display id: BBa_K1356004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitric oxide reductase (norC) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for the nitrite reductase (nirS) that converts nitrite (NO2-) into nitric oxide (NO). It is cloned into the pSB1C3
mutable notes: Nothing.
mutable provenance: P. aeruginosa PAO1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356004_sequence/1

BBa_K1462050
type: ComponentDefinition
persistent identity: BBa K1462050
display id: BBa_K1462050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ccr
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462050_sequence/1

BBa_K801095
type: ComponentDefinition
persistent identity: BBa K801095
display id: BBa_K801095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin - chalcone synthase (CHS) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Naringenin - chalcone synthase (CHS) coding region from ''Hypericum androsaemum''
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/11096318/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CHS
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801095_sequence/1

CHS
type: SequenceAnnotation
persistent identity: annotation2202163
display id: annotation2202163
version: 1
top level: BBa_K801095
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801095/annotation2202163/range2202163/1

https://synbiohub.org/public/igem/BBa_K801095/annotation2202163/range2202163/1
type: Range
persistent identity: range2202163
display id: range2202163
version: 1
direction: direction/forward
top level: BBa_K801095
start: 1
end: 1170
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202152
display id: annotation2202152
version: 1
top level: BBa_K801095
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801095/annotation2202152/range2202152/1

https://synbiohub.org/public/igem/BBa_K801095/annotation2202152/range2202152/1
type: Range
persistent identity: range2202152
display id: range2202152
version: 1
direction: direction/forward
top level: BBa_K801095
start: 1
end: 3
orientation: inline

rib-synth
type: ComponentDefinition
persistent identity: BBa K1172303
display id: BBa_K1172303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboflavin synthesis gene cluster from shewanella oneidensis
creator: Thorben Meyer
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This gene cluster consists of four different genes that form a single operon.
mutable notes: we eliminated three illegal restriction sites.
mutable provenance: genome of shewanella oneidensis MR-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: taa
sequence annotation: atg
sequence annotation: tga
sequence annotation: Pst1 C->T
sequence annotation: ribE
sequence annotation: EcoR1 C->T
sequence annotation: SO_3468
sequence annotation: tag
sequence annotation: ribBA
sequence annotation: ribD
sequence: https://synbiohub.org/public/igem/BBa_K1172303_sequence/1

taa
type: SequenceAnnotation
persistent identity: annotation2370724
display id: annotation2370724
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370724/range2370724/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370724/range2370724/1
type: Range
persistent identity: range2370724
display id: range2370724
version: 1
direction: direction/forward
top level: rib-synth
start: 3595
end: 3597
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370720
display id: annotation2370720
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370720/range2370720/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370720/range2370720/1
type: Range
persistent identity: range2370720
display id: range2370720
version: 1
direction: direction/forward
top level: rib-synth
start: 3121
end: 3123
orientation: inline

tga
type: SequenceAnnotation
persistent identity: annotation2370721
display id: annotation2370721
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370721/range2370721/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370721/range2370721/1
type: Range
persistent identity: range2370721
display id: range2370721
version: 1
direction: direction/forward
top level: rib-synth
start: 1144
end: 1146
orientation: inline

Pst1 C->T
type: SequenceAnnotation
persistent identity: annotation2370714
display id: annotation2370714
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370714/range2370714/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370714/range2370714/1
type: Range
persistent identity: range2370714
display id: range2370714
version: 1
direction: direction/forward
top level: rib-synth
start: 234
end: 239
orientation: inline

ribE
type: SequenceAnnotation
persistent identity: annotation2370313
display id: annotation2370313
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370313/range2370313/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370313/range2370313/1
type: Range
persistent identity: range2370313
display id: range2370313
version: 1
direction: direction/forward
top level: rib-synth
start: 3121
end: 3597
orientation: inline

EcoR1 C->T
type: SequenceAnnotation
persistent identity: annotation2370716
display id: annotation2370716
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370716/range2370716/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370716/range2370716/1
type: Range
persistent identity: range2370716
display id: range2370716
version: 1
direction: direction/forward
top level: rib-synth
start: 1840
end: 1845
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370718
display id: annotation2370718
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370718/range2370718/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370718/range2370718/1
type: Range
persistent identity: range2370718
display id: range2370718
version: 1
direction: direction/forward
top level: rib-synth
start: 1154
end: 1156
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370717
display id: annotation2370717
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370717/range2370717/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370717/range2370717/1
type: Range
persistent identity: range2370717
display id: range2370717
version: 1
direction: direction/forward
top level: rib-synth
start: 1
end: 3
orientation: inline

SO_3468
type: SequenceAnnotation
persistent identity: annotation2370311
display id: annotation2370311
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370311/range2370311/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370311/range2370311/1
type: Range
persistent identity: range2370311
display id: range2370311
version: 1
direction: direction/forward
top level: rib-synth
start: 1154
end: 1810
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2370722
display id: annotation2370722
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370722/range2370722/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370722/range2370722/1
type: Range
persistent identity: range2370722
display id: range2370722
version: 1
direction: direction/forward
top level: rib-synth
start: 1808
end: 1810
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2370719
display id: annotation2370719
version: 1
top level: rib-synth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370719/range2370719/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370719/range2370719/1
type: Range
persistent identity: range2370719
display id: range2370719
version: 1
direction: direction/forward
top level: rib-synth
start: 1877
end: 1879
orientation: inline

ribBA
type: SequenceAnnotation
persistent identity: annotation2370312
display id: annotation2370312
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370312/range2370312/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370312/range2370312/1
type: Range
persistent identity: range2370312
display id: range2370312
version: 1
direction: direction/forward
top level: rib-synth
start: 1877
end: 2980
orientation: inline

Pst1 C->T
type: SequenceAnnotation
persistent identity: annotation2370715
display id: annotation2370715
version: 1
top level: rib-synth
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370715/range2370715/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370715/range2370715/1
type: Range
persistent identity: range2370715
display id: range2370715
version: 1
direction: direction/forward
top level: rib-synth
start: 1644
end: 1649
orientation: inline

taa
type: SequenceAnnotation
persistent identity: annotation2370723
display id: annotation2370723
version: 1
top level: rib-synth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370723/range2370723/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370723/range2370723/1
type: Range
persistent identity: range2370723
display id: range2370723
version: 1
direction: direction/forward
top level: rib-synth
start: 2978
end: 2980
orientation: inline

ribD
type: SequenceAnnotation
persistent identity: annotation2370310
display id: annotation2370310
version: 1
top level: rib-synth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172303/annotation2370310/range2370310/1

https://synbiohub.org/public/igem/BBa_K1172303/annotation2370310/range2370310/1
type: Range
persistent identity: range2370310
display id: range2370310
version: 1
direction: direction/forward
top level: rib-synth
start: 1
end: 1146
orientation: inline

BBa_K424017
type: ComponentDefinition
persistent identity: BBa K424017
display id: BBa_K424017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test plataform for rhamnosyltransferase BioBrick (Rh1AB_BB) expression in E. coli
creator: Ernesto G??mez
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: <here is a test of the composite system
mutable notes: none
mutable provenance: nope
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K424018
component: BBa_B0010
component: BBa_E0040
component: BBa_I719005
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_K424018
sequence annotation: BBa_B0012
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K424017_sequence/1

BBa_K424018
type: Component
persistent identity: component2086193
display id: component2086193
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_K424018/1

BBa_B0010
type: Component
persistent identity: component2086196
display id: component2086196
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2086195
display id: component2086195
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I719005
type: Component
persistent identity: component2086181
display id: component2086181
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0012
type: Component
persistent identity: component2086198
display id: component2086198
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2086183
display id: component2086183
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2086196
display id: annotation2086196
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086196/range2086196/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K424017/annotation2086196/range2086196/1
type: Range
persistent identity: range2086196
display id: range2086196
version: 1
direction: direction/forward
top level: BBa_K424017
start: 3220
end: 3299
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2086195
display id: annotation2086195
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086195/range2086195/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K424017/annotation2086195/range2086195/1
type: Range
persistent identity: range2086195
display id: range2086195
version: 1
direction: direction/reverse
top level: BBa_K424017
start: 2492
end: 3211
orientation: inline

BBa_K424018
type: SequenceAnnotation
persistent identity: annotation2086193
display id: annotation2086193
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086193/range2086193/1
component: BBa_K424018

https://synbiohub.org/public/igem/BBa_K424017/annotation2086193/range2086193/1
type: Range
persistent identity: range2086193
display id: range2086193
version: 1
direction: direction/reverse
top level: BBa_K424017
start: 52
end: 2485
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2086198
display id: annotation2086198
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086198/range2086198/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K424017/annotation2086198/range2086198/1
type: Range
persistent identity: range2086198
display id: range2086198
version: 1
direction: direction/forward
top level: BBa_K424017
start: 3308
end: 3348
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2086181
display id: annotation2086181
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086181/range2086181/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K424017/annotation2086181/range2086181/1
type: Range
persistent identity: range2086181
display id: range2086181
version: 1
direction: direction/reverse
top level: BBa_K424017
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2086183
display id: annotation2086183
version: 1
top level: BBa_K424017
location: https://synbiohub.org/public/igem/BBa_K424017/annotation2086183/range2086183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K424017/annotation2086183/range2086183/1
type: Range
persistent identity: range2086183
display id: range2086183
version: 1
direction: direction/reverse
top level: BBa_K424017
start: 32
end: 43
orientation: inline

BBa_K1554002
type: ComponentDefinition
persistent identity: BBa K1554002
display id: BBa_K1554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HarFAR
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fatty alcohol reductase
mutable notes: BioBrick compatible
mutable provenance: It comes from Helicoverpa armigera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554002_sequence/1

BBa_K1751000
type: ComponentDefinition
persistent identity: BBa K1751000
display id: BBa_K1751000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RgPAL1：phenylalanine ammonia lyase (PAL) coding region
creator: Tianlai Xia
created: 2015-08-02T11:00:00Z
modified: 2015-08-19T04:47:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2173_
m user id: 28395
owner id: 28395
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）, which is an??enzyme??that??catalyzes??a reaction converting L-phenylalanine??to ammonia??and??trans-cinnamic acid.PAL is the first and committed step in the??phenyl propanoid pathway??and is therefore involved in the??biosynthesis??of the??polyphenol??compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid.
mutable notes: The gene RgPAL1 can express Phenylalanine ammonia lyase（PAL）.
mutable provenance: Rehmannia glutinosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PAL coding region
sequence: https://synbiohub.org/public/igem/BBa_K1751000_sequence/1

PAL coding region
type: SequenceAnnotation
persistent identity: annotation2434421
display id: annotation2434421
version: 1
top level: BBa_K1751000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1751000/annotation2434421/range2434421/1

https://synbiohub.org/public/igem/BBa_K1751000/annotation2434421/range2434421/1
type: Range
persistent identity: range2434421
display id: range2434421
version: 1
direction: direction/forward
top level: BBa_K1751000
start: 1
end: 2127
orientation: inline

BBa_K1067002
type: ComponentDefinition
persistent identity: BBa K1067002
display id: BBa_K1067002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cytochrome A and X from Nitrosomonas europaea
creator: Kristian Davidsen and Helen Cook
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ggg
mutable notes: ggfd
mutable provenance: ggf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence annotation: start
sequence annotation: stop
sequence annotation: cycA
sequence annotation: Signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1067002_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2359456
display id: annotation2359456
version: 1
top level: BBa_K1067002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359456/range2359456/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359456/range2359456/1
type: Range
persistent identity: range2359456
display id: range2359456
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 730
end: 1431
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359450
display id: annotation2359450
version: 1
top level: BBa_K1067002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359450/range2359450/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359450/range2359450/1
type: Range
persistent identity: range2359450
display id: range2359450
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 20
end: 22
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359453
display id: annotation2359453
version: 1
top level: BBa_K1067002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359453/range2359453/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359453/range2359453/1
type: Range
persistent identity: range2359453
display id: range2359453
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 725
end: 727
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359454
display id: annotation2359454
version: 1
top level: BBa_K1067002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359454/range2359454/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359454/range2359454/1
type: Range
persistent identity: range2359454
display id: range2359454
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 1429
end: 1431
orientation: inline

cycA
type: SequenceAnnotation
persistent identity: annotation2359452
display id: annotation2359452
version: 1
top level: BBa_K1067002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359452/range2359452/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359452/range2359452/1
type: Range
persistent identity: range2359452
display id: range2359452
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 92
end: 727
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359455
display id: annotation2359455
version: 1
top level: BBa_K1067002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359455/range2359455/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359455/range2359455/1
type: Range
persistent identity: range2359455
display id: range2359455
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 730
end: 732
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2359449
display id: annotation2359449
version: 1
top level: BBa_K1067002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359449/range2359449/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359449/range2359449/1
type: Range
persistent identity: range2359449
display id: range2359449
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 20
end: 91
orientation: inline

BBa_K808011
type: ComponentDefinition
persistent identity: BBa K808011
display id: BBa_K808011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphA1: reduces the tphA2A3 complex
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808011_sequence/1

BBa_K801061
type: ComponentDefinition
persistent identity: BBa K801061
display id: BBa_K801061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-Limonene synthase 1 coding region
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: (+)-Limonene synthase 1 coding region of ''Citrus limon''. Improved version of BBa_I742111.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/21435702
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801061_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197239
display id: annotation2197239
version: 1
top level: BBa_K801061
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801061/annotation2197239/range2197239/1

https://synbiohub.org/public/igem/BBa_K801061/annotation2197239/range2197239/1
type: Range
persistent identity: range2197239
display id: range2197239
version: 1
direction: direction/forward
top level: BBa_K801061
start: 1
end: 1665
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SEC signal
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet4
sequence annotation: Beta sheet
sequence annotation: CsgD signal
sequence annotation: BBa_K342000
sequence annotation: CsgA-His2
sequence annotation: Beta sheet2
sequence annotation: Linker
sequence annotation: His tag
sequence annotation: Beta sheet5
sequence annotation: GCT into GCC
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

BBa_K2155001
type: ComponentDefinition
persistent identity: BBa K2155001
display id: BBa_K2155001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BFD-Benzoylformate Decarboxylase
creator: Bingzhao Zhuo, Yue Yang
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T02:45:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2625_
m user id: 31739
owner id: 31739
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In enzymology, a benzoylformate decarboxylase (EC 4.1.1.7) is an enzyme that catalyzes the following chemical reaction: benzoylformate + H+ ⇌ benzaldehyde + CO2 However, in our project we get an engineered benzoylformate decarboxylase, which could catalyze formaldehyde into Glycolaldehyde and Dihydroxyacetone, from CAS. If you use it to catalyze reaction in vivo, It needs one cofactor, thiamin diphosphate.
mutable notes: Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM.
mutable provenance: Benzoylformate decarboxylase(BFD) comes from Pseudomonas putida, which could cleave carbon-carbon bonds.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2155001_sequence/1

HO1
type: ComponentDefinition
persistent identity: BBa K646000
display id: BBa_K646000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hemeoxygenase with RBS
creator: Edward Moh
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _822_
m user id: 0
owner id: 10455
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
mutable notes: The gene was digested with X and S before ligation, several ligations were done to ensure correct orientation.
mutable provenance: Http://www.ncbi.nlm.nih.gov/gene/953483
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Heme Oxygenase 1
sequence: https://synbiohub.org/public/igem/BBa_K646000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2147982
display id: annotation2147982
version: 1
top level: HO1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K646000/annotation2147982/range2147982/1

https://synbiohub.org/public/igem/BBa_K646000/annotation2147982/range2147982/1
type: Range
persistent identity: range2147982
display id: range2147982
version: 1
direction: direction/forward
top level: HO1
start: 9
end: 14
orientation: inline

Heme Oxygenase 1
type: SequenceAnnotation
persistent identity: annotation2147983
display id: annotation2147983
version: 1
top level: HO1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K646000/annotation2147983/range2147983/1

https://synbiohub.org/public/igem/BBa_K646000/annotation2147983/range2147983/1
type: Range
persistent identity: range2147983
display id: range2147983
version: 1
direction: direction/forward
top level: HO1
start: 22
end: 744
orientation: inline

BBa_K1554001
type: ComponentDefinition
persistent identity: BBa K1554001
display id: BBa_K1554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AtrΔ11
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Delta11 desaturase
mutable notes: BioBrick compatible
mutable provenance: It comes from Amyelois transitella.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554001_sequence/1

BBa_K2042014
type: ComponentDefinition
persistent identity: BBa K2042014
display id: BBa_K2042014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)
creator: Toky RATOVOMANANA
created: 2016-10-03T11:00:00Z
modified: 2016-10-14T03:28:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31381
owner id: 31392
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RBS + Pct + RBS + PhaC_v4
mutable notes: Enter before 2016 deadline
mutable provenance: Enter before 2016 deadline
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2042012
component: BBa_K2042010
component: BBa_K2042017
sequence annotation: BBa_K2042017
sequence annotation: BBa_K2042010
sequence annotation: BBa_K2042012
sequence: https://synbiohub.org/public/igem/BBa_K2042014_sequence/1

BBa_K2042012
type: Component
persistent identity: component2485991
display id: component2485991
version: 1
top level: BBa_K2042014
access: public
definition: BBa_K2042012

BBa_K2042010
type: Component
persistent identity: component2485996
display id: component2485996
version: 1
top level: BBa_K2042014
access: public
definition: BBa_K2042010

BBa_K2042017
type: Component
persistent identity: component2485992
display id: component2485992
version: 1
top level: BBa_K2042014
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042017/1

BBa_K2042017
type: SequenceAnnotation
persistent identity: annotation2485992
display id: annotation2485992
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485992/range2485992/1
component: BBa_K2042017

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485992/range2485992/1
type: Range
persistent identity: range2485992
display id: range2485992
version: 1
direction: direction/reverse
top level: BBa_K2042014
start: 1694
end: 1698
orientation: inline

BBa_K2042010
type: SequenceAnnotation
persistent identity: annotation2485996
display id: annotation2485996
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485996/range2485996/1
component: BBa_K2042010

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485996/range2485996/1
type: Range
persistent identity: range2485996
display id: range2485996
version: 1
direction: direction/reverse
top level: BBa_K2042014
start: 1699
end: 3286
orientation: inline

BBa_K2042012
type: SequenceAnnotation
persistent identity: annotation2485991
display id: annotation2485991
version: 1
top level: BBa_K2042014
location: https://synbiohub.org/public/igem/BBa_K2042014/annotation2485991/range2485991/1
component: BBa_K2042012

https://synbiohub.org/public/igem/BBa_K2042014/annotation2485991/range2485991/1
type: Range
persistent identity: range2485991
display id: range2485991
version: 1
direction: direction/reverse
top level: BBa_K2042014
start: 1
end: 1693
orientation: inline

test
type: ComponentDefinition
persistent identity: BBa K1479017
display id: BBa_K1479017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: just for test
creator: tester
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: dsfadfadsfa
mutable notes: fafafdfafa
mutable provenance: fadsfasdfaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: dfdsf
sequence annotation: sdfafds
sequence: https://synbiohub.org/public/igem/BBa_K1479017_sequence/1

dfdsf
type: SequenceAnnotation
persistent identity: annotation2400466
display id: annotation2400466
version: 1
top level: test
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1
type: Range
persistent identity: range2400466
display id: range2400466
version: 1
direction: direction/forward
top level: test
start: 2
end: 21
orientation: inline

sdfafds
type: SequenceAnnotation
persistent identity: annotation2400465
display id: annotation2400465
version: 1
top level: test
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1
type: Range
persistent identity: range2400465
display id: range2400465
version: 1
direction: direction/forward
top level: test
start: 1
end: 13
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_K115002
component: BBa_J61048
component: BBa_C0040
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

BBa_K801090
type: ComponentDefinition
persistent identity: BBa K801090
display id: BBa_K801090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phenylalanine ammonia lyase (PAL) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: phenylalanine ammonia lyase (PAL) coding region from ''Rhodosporidium toruloides'' preceeded by the yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nuccore/AX366866 PMID: 1593299
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Gly instead of Glu
sequence annotation: Start codon
sequence annotation: PAL
sequence annotation: Arg instead of Lys
sequence annotation: Consensus sequence
sequence annotation: Phe instead of Gly
sequence: https://synbiohub.org/public/igem/BBa_K801090_sequence/1

Gly instead of Glu
type: SequenceAnnotation
persistent identity: annotation2201793
display id: annotation2201793
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201793/range2201793/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201793/range2201793/1
type: Range
persistent identity: range2201793
display id: range2201793
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1214
end: 1214
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201779
display id: annotation2201779
version: 1
top level: BBa_K801090
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201779/range2201779/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201779/range2201779/1
type: Range
persistent identity: range2201779
display id: range2201779
version: 1
direction: direction/forward
top level: BBa_K801090
start: 7
end: 9
orientation: inline

PAL
type: SequenceAnnotation
persistent identity: annotation2201808
display id: annotation2201808
version: 1
top level: BBa_K801090
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201808/range2201808/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201808/range2201808/1
type: Range
persistent identity: range2201808
display id: range2201808
version: 1
direction: direction/forward
top level: BBa_K801090
start: 7
end: 2154
orientation: inline

Arg instead of Lys
type: SequenceAnnotation
persistent identity: annotation2201784
display id: annotation2201784
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201784/range2201784/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201784/range2201784/1
type: Range
persistent identity: range2201784
display id: range2201784
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1007
end: 1007
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2201778
display id: annotation2201778
version: 1
top level: BBa_K801090
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201778/range2201778/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201778/range2201778/1
type: Range
persistent identity: range2201778
display id: range2201778
version: 1
direction: direction/forward
top level: BBa_K801090
start: 1
end: 6
orientation: inline

Phe instead of Gly
type: SequenceAnnotation
persistent identity: annotation2201780
display id: annotation2201780
version: 1
top level: BBa_K801090
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K801090/annotation2201780/range2201780/1

https://synbiohub.org/public/igem/BBa_K801090/annotation2201780/range2201780/1
type: Range
persistent identity: range2201780
display id: range2201780
version: 1
direction: direction/forward
top level: BBa_K801090
start: 338
end: 339
orientation: inline

BBa_K801094
type: ComponentDefinition
persistent identity: BBa K801094
display id: BBa_K801094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Naringenin - chalcone synthase(CHS) coding sequence from ''Hypericum androsaemum'' preceeded by yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/11096318/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: CHS
sequence annotation: Consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_K801094_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2202150
display id: annotation2202150
version: 1
top level: BBa_K801094
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202150/range2202150/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202150/range2202150/1
type: Range
persistent identity: range2202150
display id: range2202150
version: 1
direction: direction/forward
top level: BBa_K801094
start: 7
end: 9
orientation: inline

CHS
type: SequenceAnnotation
persistent identity: annotation2202151
display id: annotation2202151
version: 1
top level: BBa_K801094
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202151/range2202151/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202151/range2202151/1
type: Range
persistent identity: range2202151
display id: range2202151
version: 1
direction: direction/forward
top level: BBa_K801094
start: 1
end: 1176
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2202149
display id: annotation2202149
version: 1
top level: BBa_K801094
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801094/annotation2202149/range2202149/1

https://synbiohub.org/public/igem/BBa_K801094/annotation2202149/range2202149/1
type: Range
persistent identity: range2202149
display id: range2202149
version: 1
direction: direction/forward
top level: BBa_K801094
start: 1
end: 6
orientation: inline

BBa_K1462060
type: ComponentDefinition
persistent identity: BBa K1462060
display id: BBa_K1462060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AdhE2
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: 1
mutable notes: 1
mutable provenance: 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462060_sequence/1

BBa_K801073
type: ComponentDefinition
persistent identity: BBa K801073
display id: BBa_K801073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
mutable notes: -
mutable provenance: Composite part of BBa_K801010, BBa_K801070, BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CaXMT1
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: Strep-Tag
sequence annotation: ADH1 Terminator
sequence annotation: TEF2 promoter
sequence annotation: Consensus sequence
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K801073_sequence/1

CaXMT1
type: SequenceAnnotation
persistent identity: annotation2197264
display id: annotation2197264
version: 1
top level: BBa_K801073
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197264/range2197264/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197264/range2197264/1
type: Range
persistent identity: range2197264
display id: range2197264
version: 1
direction: direction/forward
top level: BBa_K801073
start: 663
end: 1778
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199450
display id: annotation2199450
version: 1
top level: BBa_K801073
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199450/range2199450/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199450/range2199450/1
type: Range
persistent identity: range2199450
display id: range2199450
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1809
end: 1814
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201181
display id: annotation2201181
version: 1
top level: BBa_K801073
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2201181/range2201181/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2201181/range2201181/1
type: Range
persistent identity: range2201181
display id: range2201181
version: 1
direction: direction/forward
top level: BBa_K801073
start: 663
end: 665
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199452
display id: annotation2199452
version: 1
top level: BBa_K801073
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199452/range2199452/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199452/range2199452/1
type: Range
persistent identity: range2199452
display id: range2199452
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1779
end: 1808
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197265
display id: annotation2197265
version: 1
top level: BBa_K801073
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197265/range2197265/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197265/range2197265/1
type: Range
persistent identity: range2197265
display id: range2197265
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1823
end: 2172
orientation: inline

TEF2 promoter
type: SequenceAnnotation
persistent identity: annotation2197263
display id: annotation2197263
version: 1
top level: BBa_K801073
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197263/range2197263/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197263/range2197263/1
type: Range
persistent identity: range2197263
display id: range2197263
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1
end: 648
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199478
display id: annotation2199478
version: 1
top level: BBa_K801073
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2199478/range2199478/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2199478/range2199478/1
type: Range
persistent identity: range2199478
display id: range2199478
version: 1
direction: direction/forward
top level: BBa_K801073
start: 657
end: 662
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation2197262
display id: annotation2197262
version: 1
top level: BBa_K801073
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801073/annotation2197262/range2197262/1

https://synbiohub.org/public/igem/BBa_K801073/annotation2197262/range2197262/1
type: Range
persistent identity: range2197262
display id: range2197262
version: 1
direction: direction/forward
top level: BBa_K801073
start: 1
end: 2172
orientation: inline

test
type: ComponentDefinition
persistent identity: BBa K1479015
display id: BBa_K1479015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Just for test
creator: tester
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long
mutable notes: consideration consideration
mutable provenance: no source no source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: dbn
sequence annotation: stop
sequence annotation: er
sequence: https://synbiohub.org/public/igem/BBa_K1479015_sequence/1

dbn
type: SequenceAnnotation
persistent identity: annotation2399607
display id: annotation2399607
version: 1
top level: test
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399607/range2399607/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399607/range2399607/1
type: Range
persistent identity: range2399607
display id: range2399607
version: 1
direction: direction/forward
top level: test
start: 4
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2399608
display id: annotation2399608
version: 1
top level: test
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399608/range2399608/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399608/range2399608/1
type: Range
persistent identity: range2399608
display id: range2399608
version: 1
direction: direction/forward
top level: test
start: 7
end: 8
orientation: inline

er
type: SequenceAnnotation
persistent identity: annotation2399606
display id: annotation2399606
version: 1
top level: test
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399606/range2399606/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399606/range2399606/1
type: Range
persistent identity: range2399606
display id: range2399606
version: 1
direction: direction/forward
top level: test
start: 1
end: 3
orientation: inline

lipA
type: ComponentDefinition
persistent identity: BBa K836003
display id: BBa_K836003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lipA from B. cepacia (codon usage optimized for E. coli)
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit). The native signal peptide was not modified.
mutable notes: The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used (not in the sequence presented here).
mutable provenance: The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization for E. coli (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K836000
component: BBa_B1006
sequence annotation: BBa_B1006
sequence annotation: BBa_K836000
sequence: https://synbiohub.org/public/igem/BBa_K836003_sequence/1

BBa_K836000
type: Component
persistent identity: component2185330
display id: component2185330
version: 1
top level: lipA
access: public
definition: LipA

BBa_B1006
type: Component
persistent identity: component2185335
display id: component2185335
version: 1
top level: lipA
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2185335
display id: annotation2185335
version: 1
top level: lipA
location: https://synbiohub.org/public/igem/BBa_K836003/annotation2185335/range2185335/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K836003/annotation2185335/range2185335/1
type: Range
persistent identity: range2185335
display id: range2185335
version: 1
direction: direction/reverse
top level: lipA
start: 1099
end: 1137
orientation: inline

BBa_K836000
type: SequenceAnnotation
persistent identity: annotation2185330
display id: annotation2185330
version: 1
top level: lipA
location: https://synbiohub.org/public/igem/BBa_K836003/annotation2185330/range2185330/1
component: BBa_K836000

https://synbiohub.org/public/igem/BBa_K836003/annotation2185330/range2185330/1
type: Range
persistent identity: range2185330
display id: range2185330
version: 1
direction: direction/reverse
top level: lipA
start: 1
end: 1098
orientation: inline

BBa_K1172305
type: ComponentDefinition
persistent identity: BBa K1172305
display id: BBa_K1172305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter
creator: Thorben Meyer
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 17241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K608006
component: BBa_K1172303
sequence annotation: BBa_K608006
sequence annotation: BBa_K1172303
sequence: https://synbiohub.org/public/igem/BBa_K1172305_sequence/1

BBa_K608006
type: Component
persistent identity: component2355203
display id: component2355203
version: 1
top level: BBa_K1172305
access: public
definition: https://synbiohub.org/public/igem/BBa_K608006/1

BBa_K1172303
type: Component
persistent identity: component2355204
display id: component2355204
version: 1
top level: BBa_K1172305
access: public
definition: rib-synth

BBa_K608006
type: SequenceAnnotation
persistent identity: annotation2355203
display id: annotation2355203
version: 1
top level: BBa_K1172305
location: https://synbiohub.org/public/igem/BBa_K1172305/annotation2355203/range2355203/1
component: BBa_K608006

https://synbiohub.org/public/igem/BBa_K1172305/annotation2355203/range2355203/1
type: Range
persistent identity: range2355203
display id: range2355203
version: 1
direction: direction/reverse
top level: BBa_K1172305
start: 1
end: 56
orientation: inline

BBa_K1172303
type: SequenceAnnotation
persistent identity: annotation2355204
display id: annotation2355204
version: 1
top level: BBa_K1172305
location: https://synbiohub.org/public/igem/BBa_K1172305/annotation2355204/range2355204/1
component: BBa_K1172303

https://synbiohub.org/public/igem/BBa_K1172305/annotation2355204/range2355204/1
type: Range
persistent identity: range2355204
display id: range2355204
version: 1
direction: direction/reverse
top level: BBa_K1172305
start: 63
end: 3659
orientation: inline

BBa_K1331006
type: ComponentDefinition
persistent identity: BBa K1331006
display id: BBa_K1331006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1331004
component: BBa_K1331001
sequence annotation: BBa_K1331001
sequence annotation: BBa_K1331004
sequence: https://synbiohub.org/public/igem/BBa_K1331006_sequence/1

BBa_K1331004
type: Component
persistent identity: component2386717
display id: component2386717
version: 1
top level: BBa_K1331006
access: public
definition: BBa_K1331004

BBa_K1331001
type: Component
persistent identity: component2386715
display id: component2386715
version: 1
top level: BBa_K1331006
access: public
definition: BBa_K1331001

BBa_K1331001
type: SequenceAnnotation
persistent identity: annotation2386715
display id: annotation2386715
version: 1
top level: BBa_K1331006
location: https://synbiohub.org/public/igem/BBa_K1331006/annotation2386715/range2386715/1
component: BBa_K1331001

https://synbiohub.org/public/igem/BBa_K1331006/annotation2386715/range2386715/1
type: Range
persistent identity: range2386715
display id: range2386715
version: 1
direction: direction/reverse
top level: BBa_K1331006
start: 1
end: 947
orientation: inline

BBa_K1331004
type: SequenceAnnotation
persistent identity: annotation2386717
display id: annotation2386717
version: 1
top level: BBa_K1331006
location: https://synbiohub.org/public/igem/BBa_K1331006/annotation2386717/range2386717/1
component: BBa_K1331004

https://synbiohub.org/public/igem/BBa_K1331006/annotation2386717/range2386717/1
type: Range
persistent identity: range2386717
display id: range2386717
version: 1
direction: direction/reverse
top level: BBa_K1331006
start: 956
end: 2262
orientation: inline

BBa_K1331002
type: ComponentDefinition
persistent identity: BBa K1331002
display id: BBa_K1331002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR regulatory protein from P. aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding a transcription regulator RhlR. RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). RhlR binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone). This part includes the noncoding sequence of RhlR.
mutable notes: It includes both the complete coding sequence and part of noncoding sequence of RhlR.
mutable provenance: It comes from the genomic sequence of Pseudomonas aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlR
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331002_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation2386611
display id: annotation2386611
version: 1
top level: BBa_K1331002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1
type: Range
persistent identity: range2386611
display id: range2386611
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 104
end: 826
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427979
display id: annotation2427979
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1
type: Range
persistent identity: range2427979
display id: range2427979
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 827
end: 897
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427980
display id: annotation2427980
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1
type: Range
persistent identity: range2427980
display id: range2427980
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 1
end: 103
orientation: inline

BBa_K819008
type: ComponentDefinition
persistent identity: BBa K819008
display id: BBa_K819008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Operon under T7 promoter
creator: ZHANG Zidong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: luxABCDE gene ligated to the rear of T7 promoter. When transformed into BL21(DE3) E.coli strain, add different amount IPTG to reach different final concentration of IPTG in the medium can induce different level of Lux ligt emission. No substrate is needed.
mutable notes: luxABCDE gene ligated to the rear of T7 promoter. The original Ara and P-bad were replaced with T7 promoter, which enables its usage in our project.
mutable provenance: T7 promoter and LuxCDABE with their RBS are from Partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LuxCDABEG with corresponding six RBS(from K325909)
sequence annotation: BBa_K819008
sequence: https://synbiohub.org/public/igem/BBa_K819008_sequence/1

LuxCDABEG with corresponding six RBS(from K325909)
type: SequenceAnnotation
persistent identity: annotation2197309
display id: annotation2197309
version: 1
top level: BBa_K819008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1
type: Range
persistent identity: range2197309
display id: range2197309
version: 1
direction: direction/forward
top level: BBa_K819008
start: 53
end: 6480
orientation: inline

BBa_K819008
type: SequenceAnnotation
persistent identity: annotation2197308
display id: annotation2197308
version: 1
top level: BBa_K819008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1
type: Range
persistent identity: range2197308
display id: range2197308
version: 1
direction: direction/forward
top level: BBa_K819008
start: 1
end: 46
orientation: inline

BBa_K2042010
type: ComponentDefinition
persistent identity: BBa K2042010
display id: BBa_K2042010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Pct
creator: Jonathan Tellechea
created: 2016-09-01T11:00:00Z
modified: 2016-10-12T03:07:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 32278
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K2042016
component: BBa_K2042003
component: BBa_K2042000
sequence annotation: BBa_K2042003
sequence annotation: BBa_K2042000
sequence annotation: BBa_K2042016
sequence: https://synbiohub.org/public/igem/BBa_K2042010_sequence/1

BBa_K2042016
type: Component
persistent identity: component2482790
display id: component2482790
version: 1
top level: BBa_K2042010
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042016/1

BBa_K2042003
type: Component
persistent identity: component2482789
display id: component2482789
version: 1
top level: BBa_K2042010
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042003/1

BBa_K2042000
type: Component
persistent identity: component2482791
display id: component2482791
version: 1
top level: BBa_K2042010
access: public
definition: Pct

BBa_K2042003
type: SequenceAnnotation
persistent identity: annotation2482789
display id: annotation2482789
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482789/range2482789/1
component: BBa_K2042003

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482789/range2482789/1
type: Range
persistent identity: range2482789
display id: range2482789
version: 1
direction: direction/reverse
top level: BBa_K2042010
start: 1
end: 7
orientation: inline

BBa_K2042000
type: SequenceAnnotation
persistent identity: annotation2482791
display id: annotation2482791
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482791/range2482791/1
component: BBa_K2042000

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482791/range2482791/1
type: Range
persistent identity: range2482791
display id: range2482791
version: 1
direction: direction/reverse
top level: BBa_K2042010
start: 14
end: 1588
orientation: inline

BBa_K2042016
type: SequenceAnnotation
persistent identity: annotation2482790
display id: annotation2482790
version: 1
top level: BBa_K2042010
location: https://synbiohub.org/public/igem/BBa_K2042010/annotation2482790/range2482790/1
component: BBa_K2042016

https://synbiohub.org/public/igem/BBa_K2042010/annotation2482790/range2482790/1
type: Range
persistent identity: range2482790
display id: range2482790
version: 1
direction: direction/reverse
top level: BBa_K2042010
start: 8
end: 13
orientation: inline

BBa_K1331004
type: ComponentDefinition
persistent identity: BBa K1331004
display id: BBa_K1331004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable notes: Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable provenance: Pseudomonas aeruginosa SQ6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlB
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331004_sequence/1

RhlB
type: SequenceAnnotation
persistent identity: annotation2434209
display id: annotation2434209
version: 1
top level: BBa_K1331004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1
type: Range
persistent identity: range2434209
display id: range2434209
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 7
end: 1287
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434210
display id: annotation2434210
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1
type: Range
persistent identity: range2434210
display id: range2434210
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1288
end: 1307
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434208
display id: annotation2434208
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1
type: Range
persistent identity: range2434208
display id: range2434208
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1
end: 6
orientation: inline

BBa_K1598001
type: ComponentDefinition
persistent identity: BBa K1598001
display id: BBa_K1598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPH1 (Tryptophan hydroxylase 1)
creator: Marta Napiorkowska
created: 2015-06-17T11:00:00Z
modified: 2015-08-27T02:25:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2015_
m user id: 27180
owner id: 27256
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Converts the amino acid tryptophan into 5-HTP, which is a precursor to serotonin. Soon to be edit
mutable notes: Optimised for E.coli, removed illegal restriction enzyme sites
mutable provenance: Human (Homo sapiens)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1598001_sequence/1

BBa_K646003
type: ComponentDefinition
persistent identity: BBa K646003
display id: BBa_K646003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter with Hemeoxygenase
creator: David Dubow
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _822_
m user id: 0
owner id: 10455
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P. Successful expression should result in green colonies due to the presence of biliverdin.
mutable notes: None.
mutable provenance: BBa_K646000 and BBa_I719005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I719005
component: BBa_K646000
sequence annotation: BBa_K646000
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_K646003_sequence/1

BBa_I719005
type: Component
persistent identity: component2152628
display id: component2152628
version: 1
top level: BBa_K646003
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K646000
type: Component
persistent identity: component2152631
display id: component2152631
version: 1
top level: BBa_K646003
access: public
definition: HO1

BBa_K646000
type: SequenceAnnotation
persistent identity: annotation2152631
display id: annotation2152631
version: 1
top level: BBa_K646003
location: https://synbiohub.org/public/igem/BBa_K646003/annotation2152631/range2152631/1
component: BBa_K646000

https://synbiohub.org/public/igem/BBa_K646003/annotation2152631/range2152631/1
type: Range
persistent identity: range2152631
display id: range2152631
version: 1
direction: direction/reverse
top level: BBa_K646003
start: 32
end: 775
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2152628
display id: annotation2152628
version: 1
top level: BBa_K646003
location: https://synbiohub.org/public/igem/BBa_K646003/annotation2152628/range2152628/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K646003/annotation2152628/range2152628/1
type: Range
persistent identity: range2152628
display id: range2152628
version: 1
direction: direction/reverse
top level: BBa_K646003
start: 1
end: 23
orientation: inline

BBa_K1067005
type: ComponentDefinition
persistent identity: BBa K1067005
display id: BBa_K1067005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrification system
creator: Kristian Davidsen and Helen Cook
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: llll
mutable notes: llll
mutable provenance: llll
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1067002
component: BBa_K1067004
component: BBa_K1067003
sequence annotation: BBa_K1067004
sequence annotation: BBa_K1067003
sequence annotation: BBa_K1067002
sequence: https://synbiohub.org/public/igem/BBa_K1067005_sequence/1

BBa_K1067002
type: Component
persistent identity: component2359733
display id: component2359733
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067002

BBa_K1067004
type: Component
persistent identity: component2359750
display id: component2359750
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067004

BBa_K1067003
type: Component
persistent identity: component2359743
display id: component2359743
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067003

BBa_K1067004
type: SequenceAnnotation
persistent identity: annotation2359750
display id: annotation2359750
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359750/range2359750/1
component: BBa_K1067004

https://synbiohub.org/public/igem/BBa_K1067005/annotation2359750/range2359750/1
type: Range
persistent identity: range2359750
display id: range2359750
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 4528
end: 7346
orientation: inline

BBa_K1067003
type: SequenceAnnotation
persistent identity: annotation2359743
display id: annotation2359743
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359743/range2359743/1
component: BBa_K1067003

https://synbiohub.org/public/igem/BBa_K1067005/annotation2359743/range2359743/1
type: Range
persistent identity: range2359743
display id: range2359743
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 1448
end: 4521
orientation: inline

BBa_K1067002
type: SequenceAnnotation
persistent identity: annotation2359733
display id: annotation2359733
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359733/range2359733/1
component: BBa_K1067002

https://synbiohub.org/public/igem/BBa_K1067005/annotation2359733/range2359733/1
type: Range
persistent identity: range2359733
display id: range2359733
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 1
end: 1441
orientation: inline

BBa_K108017
type: ComponentDefinition
persistent identity: BBa K108017
display id: BBa_K108017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhaP
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.
mutable provenance: Alcaligenes eutrophus H16 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PhaP
sequence: https://synbiohub.org/public/igem/BBa_K108017_sequence/1

PhaP
type: SequenceAnnotation
persistent identity: annotation1986962
display id: annotation1986962
version: 1
top level: BBa_K108017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108017/annotation1986962/range1986962/1

https://synbiohub.org/public/igem/BBa_K108017/annotation1986962/range1986962/1
type: Range
persistent identity: range1986962
display id: range1986962
version: 1
direction: direction/forward
top level: BBa_K108017
start: 1
end: 576
orientation: inline

BBa_K801062
type: ComponentDefinition
persistent identity: BBa K801062
display id: BBa_K801062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express Citrus limon (+)-limonene synthase 1 in yeast. The expression is controled by the TEF1 promoter and the CYC1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the ''Citrus limon'' (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K801061
sequence annotation: TEF1 promotor
sequence annotation: Stop codon
sequence annotation: Cyc terminator
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801062_sequence/1

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197879
display id: annotation2197879
version: 1
top level: BBa_K801062
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197879/range2197879/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197879/range2197879/1
type: Range
persistent identity: range2197879
display id: range2197879
version: 1
direction: direction/forward
top level: BBa_K801062
start: 462
end: 2126
orientation: inline

TEF1 promotor
type: SequenceAnnotation
persistent identity: annotation2197883
display id: annotation2197883
version: 1
top level: BBa_K801062
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197883/range2197883/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197883/range2197883/1
type: Range
persistent identity: range2197883
display id: range2197883
version: 1
direction: direction/forward
top level: BBa_K801062
start: 1
end: 457
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2197885
display id: annotation2197885
version: 1
top level: BBa_K801062
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197885/range2197885/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197885/range2197885/1
type: Range
persistent identity: range2197885
display id: range2197885
version: 1
direction: direction/forward
top level: BBa_K801062
start: 2133
end: 2137
orientation: inline

Cyc terminator
type: SequenceAnnotation
persistent identity: annotation2197884
display id: annotation2197884
version: 1
top level: BBa_K801062
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197884/range2197884/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197884/range2197884/1
type: Range
persistent identity: range2197884
display id: range2197884
version: 1
direction: direction/forward
top level: BBa_K801062
start: 2143
end: 2424
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197878
display id: annotation2197878
version: 1
top level: BBa_K801062
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801062/annotation2197878/range2197878/1

https://synbiohub.org/public/igem/BBa_K801062/annotation2197878/range2197878/1
type: Range
persistent identity: range2197878
display id: range2197878
version: 1
direction: direction/forward
top level: BBa_K801062
start: 462
end: 2126
orientation: inline

BBa_K552004
type: ComponentDefinition
persistent identity: BBa K552004
display id: BBa_K552004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCD1 Gene-Codon Optimized for Yeast
creator: Jonathan Herrmann
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:40Z
discontinued: false
dominant: false
group u list: _720_
m user id: 0
owner id: 8813
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This DNA sequence codes for the enzyme CCD1, also known as carotenoid cleavage dioxygenase 1. CCD1 cleaves beta-caroten to form two molecules of beta-ionone. This enzyme completes the beta-ionone biosynthesis pathway in yeast. This sequence also includes a PGK promoter and an ADH1 terminator.
mutable notes: While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.
mutable provenance: The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Start Codon
sequence annotation: ADH1 Terminator
sequence annotation: Stop Codon
sequence annotation: CCD1
sequence annotation: PGK Promoter
sequence: https://synbiohub.org/public/igem/BBa_K552004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2145139
display id: annotation2145139
version: 1
top level: BBa_K552004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145139/range2145139/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145139/range2145139/1
type: Range
persistent identity: range2145139
display id: range2145139
version: 1
direction: direction/forward
top level: BBa_K552004
start: 381
end: 383
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2145144
display id: annotation2145144
version: 1
top level: BBa_K552004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145144/range2145144/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145144/range2145144/1
type: Range
persistent identity: range2145144
display id: range2145144
version: 1
direction: direction/forward
top level: BBa_K552004
start: 2012
end: 2141
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2145143
display id: annotation2145143
version: 1
top level: BBa_K552004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145143/range2145143/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145143/range2145143/1
type: Range
persistent identity: range2145143
display id: range2145143
version: 1
direction: direction/forward
top level: BBa_K552004
start: 2010
end: 2012
orientation: inline

CCD1
type: SequenceAnnotation
persistent identity: annotation2145142
display id: annotation2145142
version: 1
top level: BBa_K552004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145142/range2145142/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145142/range2145142/1
type: Range
persistent identity: range2145142
display id: range2145142
version: 1
direction: direction/forward
top level: BBa_K552004
start: 381
end: 2012
orientation: inline

PGK Promoter
type: SequenceAnnotation
persistent identity: annotation2145140
display id: annotation2145140
version: 1
top level: BBa_K552004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K552004/annotation2145140/range2145140/1

https://synbiohub.org/public/igem/BBa_K552004/annotation2145140/range2145140/1
type: Range
persistent identity: range2145140
display id: range2145140
version: 1
direction: direction/forward
top level: BBa_K552004
start: 1
end: 380
orientation: inline

BBa_K2042011
type: ComponentDefinition
persistent identity: BBa K2042011
display id: BBa_K2042011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS +PhaC_resinovorans
creator: Jonathan Tellechea
created: 2016-09-01T11:00:00Z
modified: 2016-10-10T09:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 32278
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K2042001
component: BBa_K2042003
component: BBa_K2042016
sequence annotation: BBa_K2042001
sequence annotation: BBa_K2042003
sequence annotation: BBa_K2042016
sequence: https://synbiohub.org/public/igem/BBa_K2042011_sequence/1

BBa_K2042001
type: Component
persistent identity: component2482794
display id: component2482794
version: 1
top level: BBa_K2042011
access: public
definition: BBa_K2042001

BBa_K2042003
type: Component
persistent identity: component2482792
display id: component2482792
version: 1
top level: BBa_K2042011
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042003/1

BBa_K2042016
type: Component
persistent identity: component2482793
display id: component2482793
version: 1
top level: BBa_K2042011
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042016/1

BBa_K2042001
type: SequenceAnnotation
persistent identity: annotation2482794
display id: annotation2482794
version: 1
top level: BBa_K2042011
location: https://synbiohub.org/public/igem/BBa_K2042011/annotation2482794/range2482794/1
component: BBa_K2042001

https://synbiohub.org/public/igem/BBa_K2042011/annotation2482794/range2482794/1
type: Range
persistent identity: range2482794
display id: range2482794
version: 1
direction: direction/reverse
top level: BBa_K2042011
start: 14
end: 1693
orientation: inline

BBa_K2042003
type: SequenceAnnotation
persistent identity: annotation2482792
display id: annotation2482792
version: 1
top level: BBa_K2042011
location: https://synbiohub.org/public/igem/BBa_K2042011/annotation2482792/range2482792/1
component: BBa_K2042003

https://synbiohub.org/public/igem/BBa_K2042011/annotation2482792/range2482792/1
type: Range
persistent identity: range2482792
display id: range2482792
version: 1
direction: direction/reverse
top level: BBa_K2042011
start: 1
end: 7
orientation: inline

BBa_K2042016
type: SequenceAnnotation
persistent identity: annotation2482793
display id: annotation2482793
version: 1
top level: BBa_K2042011
location: https://synbiohub.org/public/igem/BBa_K2042011/annotation2482793/range2482793/1
component: BBa_K2042016

https://synbiohub.org/public/igem/BBa_K2042011/annotation2482793/range2482793/1
type: Range
persistent identity: range2482793
display id: range2482793
version: 1
direction: direction/reverse
top level: BBa_K2042011
start: 8
end: 13
orientation: inline

BBa_K801093
type: ComponentDefinition
persistent identity: BBa K801093
display id: BBa_K801093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4-coumarate--coenzyme A ligase (4CL) coding region
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 4-coumarate--coenzyme A ligase (4CL) coding region from ''Arabidopsis thaliana''.
mutable notes: -
mutable provenance: Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA http://www.ncbi.nlm.nih.gov/nucleotide/609339/ PMID: 15932991
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Start codon
sequence annotation: 4CL
sequence: https://synbiohub.org/public/igem/BBa_K801093_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2202141
display id: annotation2202141
version: 1
top level: BBa_K801093
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202141/range2202141/1
type: Range
persistent identity: range2202141
display id: range2202141
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1684
end: 1686
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202140
display id: annotation2202140
version: 1
top level: BBa_K801093
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202140/range2202140/1
type: Range
persistent identity: range2202140
display id: range2202140
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 3
orientation: inline

4CL
type: SequenceAnnotation
persistent identity: annotation2202143
display id: annotation2202143
version: 1
top level: BBa_K801093
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1

https://synbiohub.org/public/igem/BBa_K801093/annotation2202143/range2202143/1
type: Range
persistent identity: range2202143
display id: range2202143
version: 1
direction: direction/forward
top level: BBa_K801093
start: 1
end: 1683
orientation: inline

BBa_K801080
type: ComponentDefinition
persistent identity: BBa K801080
display id: BBa_K801080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prepro-Thaumatin, yeast codon optimized
creator: Alois Br??uer
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Coding region for Prepro-Thaumatin. Codon optimized for yeast.
mutable notes: Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.
mutable provenance: Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Preprothaumatin
sequence annotation: Pre-signal sequence
sequence annotation: Pro-signal sequence
sequence: https://synbiohub.org/public/igem/BBa_K801080_sequence/1

Preprothaumatin
type: SequenceAnnotation
persistent identity: annotation2343093
display id: annotation2343093
version: 1
top level: BBa_K801080
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343093/range2343093/1
type: Range
persistent identity: range2343093
display id: range2343093
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 705
orientation: inline

Pre-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343094
display id: annotation2343094
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343094/range2343094/1
type: Range
persistent identity: range2343094
display id: range2343094
version: 1
direction: direction/forward
top level: BBa_K801080
start: 1
end: 67
orientation: inline

Pro-signal sequence
type: SequenceAnnotation
persistent identity: annotation2343095
display id: annotation2343095
version: 1
top level: BBa_K801080
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1

https://synbiohub.org/public/igem/BBa_K801080/annotation2343095/range2343095/1
type: Range
persistent identity: range2343095
display id: range2343095
version: 1
direction: direction/forward
top level: BBa_K801080
start: 688
end: 705
orientation: inline

BBa_K1687000
type: ComponentDefinition
persistent identity: BBa K1687000
display id: BBa_K1687000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E.C. 1.7.7.2 Generator
creator: 2014&2015 Shenzhen Middle School
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T03:58:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2105_
m user id: 24745
owner id: 24745
owning group id: 9
status: status/Planning
bookmark: true
mutable description: 00000000000000000000000
mutable notes: 00000000000000
mutable provenance: 1000000000000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EC 1.7.7.2 generator
sequence: https://synbiohub.org/public/igem/BBa_K1687000_sequence/1

EC 1.7.7.2 generator
type: SequenceAnnotation
persistent identity: annotation2458757
display id: annotation2458757
version: 1
top level: BBa_K1687000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1687000/annotation2458757/range2458757/1

https://synbiohub.org/public/igem/BBa_K1687000/annotation2458757/range2458757/1
type: Range
persistent identity: range2458757
display id: range2458757
version: 1
direction: direction/forward
top level: BBa_K1687000
start: 1
end: 1953
orientation: inline

BBa_K1497015
type: ComponentDefinition
persistent identity: BBa K1497015
display id: BBa_K1497015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon - T7-B0034-F3H-B0034-DFR-B0034-eANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen, Sven Jager
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I712074
component: BBa_K1497013
component: BBa_K1497012
component: BBa_K1497002
sequence annotation: BBa_I712074
sequence annotation: BBa_K1497002
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497013
sequence: https://synbiohub.org/public/igem/BBa_K1497015_sequence/1

BBa_I712074
type: Component
persistent identity: component2421892
display id: component2421892
version: 1
top level: BBa_K1497015
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K1497013
type: Component
persistent identity: component2421900
display id: component2421900
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497013

BBa_K1497012
type: Component
persistent identity: component2421896
display id: component2421896
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497012

BBa_K1497002
type: Component
persistent identity: component2421901
display id: component2421901
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497002

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2421892
display id: annotation2421892
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421892/range2421892/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1497015/annotation2421892/range2421892/1
type: Range
persistent identity: range2421892
display id: range2421892
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 1
end: 46
orientation: inline

BBa_K1497002
type: SequenceAnnotation
persistent identity: annotation2421901
display id: annotation2421901
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421901/range2421901/1
component: BBa_K1497002

https://synbiohub.org/public/igem/BBa_K1497015/annotation2421901/range2421901/1
type: Range
persistent identity: range2421901
display id: range2421901
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 2276
end: 3243
orientation: inline

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2421896
display id: annotation2421896
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421896/range2421896/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497015/annotation2421896/range2421896/1
type: Range
persistent identity: range2421896
display id: range2421896
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 55
end: 1179
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2421900
display id: annotation2421900
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421900/range2421900/1
component: BBa_K1497013

https://synbiohub.org/public/igem/BBa_K1497015/annotation2421900/range2421900/1
type: Range
persistent identity: range2421900
display id: range2421900
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 1188
end: 2267
orientation: inline

BBa_K1660001
type: ComponentDefinition
persistent identity: BBa K1660001
display id: BBa_K1660001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rMpL
creator: Dai Yuanyi
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T07:03:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes a nematotoxic lectin from the parasol mushroom( Macrolepiota procera) under the control of pBAD promoter.
mutable notes: We searched the cDNA of MpL, and optimized the codon for E. coli.
mutable provenance: It comes from Macrolepiota procera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop codon
sequence annotation: K206000
sequence annotation: B0034
sequence annotation: start codon
sequence annotation: rMpL
sequence: https://synbiohub.org/public/igem/BBa_K1660001_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2445590
display id: annotation2445590
version: 1
top level: BBa_K1660001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660001/annotation2445590/range2445590/1

https://synbiohub.org/public/igem/BBa_K1660001/annotation2445590/range2445590/1
type: Range
persistent identity: range2445590
display id: range2445590
version: 1
direction: direction/forward
top level: BBa_K1660001
start: 580
end: 582
orientation: inline

K206000
type: SequenceAnnotation
persistent identity: annotation2445586
display id: annotation2445586
version: 1
top level: BBa_K1660001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660001/annotation2445586/range2445586/1

https://synbiohub.org/public/igem/BBa_K1660001/annotation2445586/range2445586/1
type: Range
persistent identity: range2445586
display id: range2445586
version: 1
direction: direction/forward
top level: BBa_K1660001
start: 1
end: 130
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445587
display id: annotation2445587
version: 1
top level: BBa_K1660001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660001/annotation2445587/range2445587/1

https://synbiohub.org/public/igem/BBa_K1660001/annotation2445587/range2445587/1
type: Range
persistent identity: range2445587
display id: range2445587
version: 1
direction: direction/reverse
top level: BBa_K1660001
start: 139
end: 150
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445589
display id: annotation2445589
version: 1
top level: BBa_K1660001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660001/annotation2445589/range2445589/1

https://synbiohub.org/public/igem/BBa_K1660001/annotation2445589/range2445589/1
type: Range
persistent identity: range2445589
display id: range2445589
version: 1
direction: direction/forward
top level: BBa_K1660001
start: 157
end: 159
orientation: inline

rMpL
type: SequenceAnnotation
persistent identity: annotation2445592
display id: annotation2445592
version: 1
top level: BBa_K1660001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660001/annotation2445592/range2445592/1

https://synbiohub.org/public/igem/BBa_K1660001/annotation2445592/range2445592/1
type: Range
persistent identity: range2445592
display id: range2445592
version: 1
direction: direction/forward
top level: BBa_K1660001
start: 157
end: 582
orientation: inline

BBa_K518009
type: ComponentDefinition
persistent identity: BBa K518009
display id: BBa_K518009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AspA expression cassette (no promoter)
creator: Masato Ohgishi
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli.
mutable notes: None.
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_C0083
component: BBa_B0014
sequence annotation: BBa_B0032
sequence annotation: BBa_C0083
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K518009_sequence/1

BBa_B0032
type: Component
persistent identity: component2250719
display id: component2250719
version: 1
top level: BBa_K518009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0083
type: Component
persistent identity: component2250729
display id: component2250729
version: 1
top level: BBa_K518009
access: public
definition: https://synbiohub.org/public/igem/BBa_C0083/1

BBa_B0014
type: Component
persistent identity: component2250736
display id: component2250736
version: 1
top level: BBa_K518009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250719
display id: annotation2250719
version: 1
top level: BBa_K518009
location: https://synbiohub.org/public/igem/BBa_K518009/annotation2250719/range2250719/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518009/annotation2250719/range2250719/1
type: Range
persistent identity: range2250719
display id: range2250719
version: 1
direction: direction/forward
top level: BBa_K518009
start: 1
end: 13
orientation: inline

BBa_C0083
type: SequenceAnnotation
persistent identity: annotation2250729
display id: annotation2250729
version: 1
top level: BBa_K518009
location: https://synbiohub.org/public/igem/BBa_K518009/annotation2250729/range2250729/1
component: BBa_C0083

https://synbiohub.org/public/igem/BBa_K518009/annotation2250729/range2250729/1
type: Range
persistent identity: range2250729
display id: range2250729
version: 1
direction: direction/reverse
top level: BBa_K518009
start: 20
end: 1562
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2250736
display id: annotation2250736
version: 1
top level: BBa_K518009
location: https://synbiohub.org/public/igem/BBa_K518009/annotation2250736/range2250736/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518009/annotation2250736/range2250736/1
type: Range
persistent identity: range2250736
display id: range2250736
version: 1
direction: direction/reverse
top level: BBa_K518009
start: 1571
end: 1665
orientation: inline

BBa_K1322231
type: ComponentDefinition
persistent identity: BBa K1322231
display id: BBa_K1322231
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Santalene Synthase
creator: Jarrod Shilts
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1697_
m user id: 0
owner id: 19415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K1322231 is a codon-optimized biobrick part encoding the gene for alpha-santalene synthase (EC 4.2.3.82). The enzyme catalyze the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the sesquiterpene (+)-alpha-santelene in a single step. Traces of (-)-beta-santalene and bergamontene have previously been shown to be produced by this enzyme as well. The gene, derived from a relative of the exotic sandalwood tree, has been demonstrated to produce functional terpene product in both yeast (Scalcinati et al 2012) and E. coli (data pending). This is possible due to several endogenous pathways that produce FPP as an intermediate, including the MEV and MEP pathways, In addition to being a prized fragrance, with what is often described as a warm, sweet woody scent, the sandalwood oil has been investigated for a number of other practical applications, including as a chemoprotective against carcinogenesis (Banaerjee, Ecavade, and Rao 1993) and inhibitors of viral reproduction (Koch et al 2008). References: Scalcinati et al.: Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae. Microbial Cell Factories 2012 11:117. Banerjee, Ecavade and Rao: Modulatory influence of sandalwood oil on mouse hepatic glutathione S-transferase activity and acid soluble sulphydryl level. Cancer Letters, 68 (1993) 105 - 109 Koch et al: Inhibitory effect of essential oils against herpes simplex virus type 2. Phytomedicine 2008;15(1-2):71-8.
mutable notes: Two sites on the original gene sequence had to be changed by selected sense mutation codons in order to make the part RFC10 compatible.
mutable provenance: The sequence comes from genomic DNA taken from the santalum genus of plant. It was codon optimized for expression in Saccharomyces cerevisiae. The part also contains a yeast consensus sequence before the start codon, a C-terminal strep tag, and biobrick prefixes and suffixes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Santalene Synthase
sequence annotation: Strep Tag
sequence annotation: Yeast Consensus Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1322231_sequence/1

Santalene Synthase
type: SequenceAnnotation
persistent identity: annotation2421998
display id: annotation2421998
version: 1
top level: BBa_K1322231
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1322231/annotation2421998/range2421998/1

https://synbiohub.org/public/igem/BBa_K1322231/annotation2421998/range2421998/1
type: Range
persistent identity: range2421998
display id: range2421998
version: 1
direction: direction/forward
top level: BBa_K1322231
start: 14
end: 1667
orientation: inline

Strep Tag
type: SequenceAnnotation
persistent identity: annotation2421999
display id: annotation2421999
version: 1
top level: BBa_K1322231
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1322231/annotation2421999/range2421999/1

https://synbiohub.org/public/igem/BBa_K1322231/annotation2421999/range2421999/1
type: Range
persistent identity: range2421999
display id: range2421999
version: 1
direction: direction/forward
top level: BBa_K1322231
start: 1668
end: 1692
orientation: inline

Yeast Consensus Sequence
type: SequenceAnnotation
persistent identity: annotation2421997
display id: annotation2421997
version: 1
top level: BBa_K1322231
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1322231/annotation2421997/range2421997/1

https://synbiohub.org/public/igem/BBa_K1322231/annotation2421997/range2421997/1
type: Range
persistent identity: range2421997
display id: range2421997
version: 1
direction: direction/forward
top level: BBa_K1322231
start: 7
end: 13
orientation: inline

norB
type: ComponentDefinition
persistent identity: BBa K1356005
display id: BBa_K1356005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitric oxide reductase (norB) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blah
mutable notes: Mutagenesis
mutable provenance: P.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356005_sequence/1

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61048
component: BBa_K332031
component: BBa_R0040
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K332031
sequence annotation: BBa_E0040
sequence annotation: BBa_J61048
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_K801063
type: ComponentDefinition
persistent identity: BBa K801063
display id: BBa_K801063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-limonene synthase 1 expression cassette for yeast
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to express Citrus limon (+)-limonene synthase 1 in yeast. The expression is controled by the yeast TEF1 promoter and the yeast TEF1 terminator.
mutable notes: -
mutable provenance: This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Stop codon
sequence annotation: BBa_K801061
sequence annotation: Coding sequence
sequence annotation: TEF1 promotor
sequence annotation: TEF1 terminator
sequence annotation: Age1 restriction site
sequence: https://synbiohub.org/public/igem/BBa_K801063_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2197864
display id: annotation2197864
version: 1
top level: BBa_K801063
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197864/range2197864/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197864/range2197864/1
type: Range
persistent identity: range2197864
display id: range2197864
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2133
end: 2135
orientation: inline

BBa_K801061
type: SequenceAnnotation
persistent identity: annotation2197850
display id: annotation2197850
version: 1
top level: BBa_K801063
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197850/range2197850/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197850/range2197850/1
type: Range
persistent identity: range2197850
display id: range2197850
version: 1
direction: direction/forward
top level: BBa_K801063
start: 462
end: 2126
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197849
display id: annotation2197849
version: 1
top level: BBa_K801063
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197849/range2197849/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197849/range2197849/1
type: Range
persistent identity: range2197849
display id: range2197849
version: 1
direction: direction/forward
top level: BBa_K801063
start: 462
end: 2126
orientation: inline

TEF1 promotor
type: SequenceAnnotation
persistent identity: annotation2197858
display id: annotation2197858
version: 1
top level: BBa_K801063
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197858/range2197858/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197858/range2197858/1
type: Range
persistent identity: range2197858
display id: range2197858
version: 1
direction: direction/forward
top level: BBa_K801063
start: 1
end: 456
orientation: inline

TEF1 terminator
type: SequenceAnnotation
persistent identity: annotation2197861
display id: annotation2197861
version: 1
top level: BBa_K801063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197861/range2197861/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197861/range2197861/1
type: Range
persistent identity: range2197861
display id: range2197861
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2143
end: 2652
orientation: inline

Age1 restriction site
type: SequenceAnnotation
persistent identity: annotation2197875
display id: annotation2197875
version: 1
top level: BBa_K801063
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801063/annotation2197875/range2197875/1

https://synbiohub.org/public/igem/BBa_K801063/annotation2197875/range2197875/1
type: Range
persistent identity: range2197875
display id: range2197875
version: 1
direction: direction/forward
top level: BBa_K801063
start: 2127
end: 2132
orientation: inline

BBa_K1497012
type: ComponentDefinition
persistent identity: BBa K1497012
display id: BBa_K1497012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1497009
sequence annotation: BBa_B0034
sequence annotation: BBa_K1497009
sequence: https://synbiohub.org/public/igem/BBa_K1497012_sequence/1

BBa_B0034
type: Component
persistent identity: component2398819
display id: component2398819
version: 1
top level: BBa_K1497012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497009
type: Component
persistent identity: component2398820
display id: component2398820
version: 1
top level: BBa_K1497012
access: public
definition: BBa_K1497009

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398819
display id: annotation2398819
version: 1
top level: BBa_K1497012
location: https://synbiohub.org/public/igem/BBa_K1497012/annotation2398819/range2398819/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497012/annotation2398819/range2398819/1
type: Range
persistent identity: range2398819
display id: range2398819
version: 1
direction: direction/reverse
top level: BBa_K1497012
start: 1
end: 12
orientation: inline

BBa_K1497009
type: SequenceAnnotation
persistent identity: annotation2398820
display id: annotation2398820
version: 1
top level: BBa_K1497012
location: https://synbiohub.org/public/igem/BBa_K1497012/annotation2398820/range2398820/1
component: BBa_K1497009

https://synbiohub.org/public/igem/BBa_K1497012/annotation2398820/range2398820/1
type: Range
persistent identity: range2398820
display id: range2398820
version: 1
direction: direction/reverse
top level: BBa_K1497012
start: 19
end: 1125
orientation: inline

BBa_M36008
type: ComponentDefinition
persistent identity: BBa M36008
display id: BBa_M36008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: predicted glycogen synthesis protein
creator: Jesse Palmer, Elliot Lui
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for a protein involved in the biosynthesis of glycogen. Overproduction stimulates glycogen synthesis. Influences the first two enzymes in the e coli glycogen biosynthesis pathway (glucose-1-phosphate adenylyltransferase, glycogen synthase). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-REG-SUMMARY&object=EG11381 for more information.
mutable provenance: Genomic sequence glgS as provided on EcoCyc database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M36008_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2119218
display id: annotation2119218
version: 1
top level: BBa_M36008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36008/annotation2119218/range2119218/1

https://synbiohub.org/public/igem/BBa_M36008/annotation2119218/range2119218/1
type: Range
persistent identity: range2119218
display id: range2119218
version: 1
direction: direction/forward
top level: BBa_M36008
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119219
display id: annotation2119219
version: 1
top level: BBa_M36008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36008/annotation2119219/range2119219/1

https://synbiohub.org/public/igem/BBa_M36008/annotation2119219/range2119219/1
type: Range
persistent identity: range2119219
display id: range2119219
version: 1
direction: direction/forward
top level: BBa_M36008
start: 199
end: 201
orientation: inline

BBa_K1149002
type: ComponentDefinition
persistent identity: BBa K1149002
display id: BBa_K1149002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB-EstCS2-his expression (GFP reporter)
creator: Margarita Kopniczky
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1461_
m user id: 0
owner id: 17968
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K206000
component: BBa_S05214
component: BBa_B0034
sequence annotation: BBa_S05214
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K1149002_sequence/1

BBa_K206000
type: Component
persistent identity: component2343315
display id: component2343315
version: 1
top level: BBa_K1149002
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_S05214
type: Component
persistent identity: component2343325
display id: component2343325
version: 1
top level: BBa_K1149002
access: public
definition: https://synbiohub.org/public/igem/BBa_S05214/1

BBa_B0034
type: Component
persistent identity: component2343317
display id: component2343317
version: 1
top level: BBa_K1149002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_S05214
type: SequenceAnnotation
persistent identity: annotation2343325
display id: annotation2343325
version: 1
top level: BBa_K1149002
location: https://synbiohub.org/public/igem/BBa_K1149002/annotation2343325/range2343325/1
component: BBa_S05214

https://synbiohub.org/public/igem/BBa_K1149002/annotation2343325/range2343325/1
type: Range
persistent identity: range2343325
display id: range2343325
version: 1
direction: direction/reverse
top level: BBa_K1149002
start: 157
end: 2703
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2343317
display id: annotation2343317
version: 1
top level: BBa_K1149002
location: https://synbiohub.org/public/igem/BBa_K1149002/annotation2343317/range2343317/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1149002/annotation2343317/range2343317/1
type: Range
persistent identity: range2343317
display id: range2343317
version: 1
direction: direction/reverse
top level: BBa_K1149002
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2343315
display id: annotation2343315
version: 1
top level: BBa_K1149002
location: https://synbiohub.org/public/igem/BBa_K1149002/annotation2343315/range2343315/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1149002/annotation2343315/range2343315/1
type: Range
persistent identity: range2343315
display id: range2343315
version: 1
direction: direction/reverse
top level: BBa_K1149002
start: 1
end: 130
orientation: inline

BBa_K801074
type: ComponentDefinition
persistent identity: BBa K801074
display id: BBa_K801074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CaMXMT1 expression cassette for yeast
creator: Dennis Hell
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11966
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yeast expression cassette for CaMXMT1 (BBa_K801071) controlled by the yeast TEF1 promoter (BBa_K319003) and the yeast ADH1 terminator (BBa_K801012).
mutable notes: -
mutable provenance: Composite part of BBa_K319003, BBa_K801071 and BBa_K801012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CaMXMT1
sequence annotation: Strep-Tag
sequence annotation: Consensus sequence
sequence annotation: Stop codon
sequence annotation: ADH1 Terminator
sequence annotation: TEF1 promoter
sequence annotation: BioBrick
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801074_sequence/1

CaMXMT1
type: SequenceAnnotation
persistent identity: annotation2197323
display id: annotation2197323
version: 1
top level: BBa_K801074
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197323/range2197323/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197323/range2197323/1
type: Range
persistent identity: range2197323
display id: range2197323
version: 1
direction: direction/forward
top level: BBa_K801074
start: 464
end: 1597
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2199502
display id: annotation2199502
version: 1
top level: BBa_K801074
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199502/range2199502/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199502/range2199502/1
type: Range
persistent identity: range2199502
display id: range2199502
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1598
end: 1627
orientation: inline

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2199499
display id: annotation2199499
version: 1
top level: BBa_K801074
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199499/range2199499/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199499/range2199499/1
type: Range
persistent identity: range2199499
display id: range2199499
version: 1
direction: direction/forward
top level: BBa_K801074
start: 458
end: 463
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2199500
display id: annotation2199500
version: 1
top level: BBa_K801074
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2199500/range2199500/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2199500/range2199500/1
type: Range
persistent identity: range2199500
display id: range2199500
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1628
end: 1633
orientation: inline

ADH1 Terminator
type: SequenceAnnotation
persistent identity: annotation2197327
display id: annotation2197327
version: 1
top level: BBa_K801074
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197327/range2197327/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197327/range2197327/1
type: Range
persistent identity: range2197327
display id: range2197327
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1642
end: 1991
orientation: inline

TEF1 promoter
type: SequenceAnnotation
persistent identity: annotation2197321
display id: annotation2197321
version: 1
top level: BBa_K801074
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197321/range2197321/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197321/range2197321/1
type: Range
persistent identity: range2197321
display id: range2197321
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1
end: 450
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation2197320
display id: annotation2197320
version: 1
top level: BBa_K801074
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2197320/range2197320/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2197320/range2197320/1
type: Range
persistent identity: range2197320
display id: range2197320
version: 1
direction: direction/forward
top level: BBa_K801074
start: 1
end: 1991
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2201346
display id: annotation2201346
version: 1
top level: BBa_K801074
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801074/annotation2201346/range2201346/1

https://synbiohub.org/public/igem/BBa_K801074/annotation2201346/range2201346/1
type: Range
persistent identity: range2201346
display id: range2201346
version: 1
direction: direction/forward
top level: BBa_K801074
start: 464
end: 466
orientation: inline

UV-WG
type: ComponentDefinition
persistent identity: BBa K994000
display id: BBa_K994000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV- Responsive Wintergreen Odor
creator: Hongmei Du
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1291_
m user id: 0
owner id: 15707
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The UV-responsive wintergreen odor generator catalyzes the conversion of the precursor salicylic acid to the wintergreen odor methyl salicylate under extensive UV irradiation. The biosynthetic device is composed of two transcriptional devices: Rec A (SOS) promoter (BBa_J22106) and a wintergreen odor enzyme generator (BBa_J45119). The odor generator takes as input extensive UV irradiation and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.
mutable notes: The primers for DNA sequncing Du-5F：5???-AGTGGAGTGCCTGGTTCAT -3???；626bp～644bp Du-3R：5???-AAATCTATTTCATATTGCTTG-3???；799bp～819bp
mutable provenance: Kit Plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_J45119
sequence annotation: stem_loop
sequence annotation: BBa_J22106
sequence annotation: BBa_B0012
sequence annotation: Start
sequence annotation: BBa_B0010
sequence annotation: RBS
sequence annotation: stop
sequence annotation: T7 TE
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: polya
sequence annotation: CDS
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K994000_sequence/1

BBa_J45119
type: SequenceAnnotation
persistent identity: annotation2330009
display id: annotation2330009
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330009/range2330009/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330009/range2330009/1
type: Range
persistent identity: range2330009
display id: range2330009
version: 1
direction: direction/reverse
top level: UV-WG
start: 201
end: 1430
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2330004
display id: annotation2330004
version: 1
top level: UV-WG
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330004/range2330004/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330004/range2330004/1
type: Range
persistent identity: range2330004
display id: range2330004
version: 1
direction: direction/forward
top level: UV-WG
start: 1313
end: 1356
orientation: inline

BBa_J22106
type: SequenceAnnotation
persistent identity: annotation2329995
display id: annotation2329995
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2329995/range2329995/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2329995/range2329995/1
type: Range
persistent identity: range2329995
display id: range2329995
version: 1
direction: direction/reverse
top level: UV-WG
start: 1
end: 192
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2330005
display id: annotation2330005
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330005/range2330005/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330005/range2330005/1
type: Range
persistent identity: range2330005
display id: range2330005
version: 1
direction: direction/forward
top level: UV-WG
start: 1390
end: 1430
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2330000
display id: annotation2330000
version: 1
top level: UV-WG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330000/range2330000/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330000/range2330000/1
type: Range
persistent identity: range2330000
display id: range2330000
version: 1
direction: direction/forward
top level: UV-WG
start: 220
end: 222
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2330003
display id: annotation2330003
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330003/range2330003/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330003/range2330003/1
type: Range
persistent identity: range2330003
display id: range2330003
version: 1
direction: direction/forward
top level: UV-WG
start: 1302
end: 1381
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329998
display id: annotation2329998
version: 1
top level: UV-WG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2329998/range2329998/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2329998/range2329998/1
type: Range
persistent identity: range2329998
display id: range2329998
version: 1
direction: direction/forward
top level: UV-WG
start: 207
end: 210
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2330008
display id: annotation2330008
version: 1
top level: UV-WG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330008/range2330008/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330008/range2330008/1
type: Range
persistent identity: range2330008
display id: range2330008
version: 1
direction: direction/forward
top level: UV-WG
start: 1423
end: 1423
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2330006
display id: annotation2330006
version: 1
top level: UV-WG
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330006/range2330006/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330006/range2330006/1
type: Range
persistent identity: range2330006
display id: range2330006
version: 1
direction: direction/forward
top level: UV-WG
start: 1397
end: 1416
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation2329997
display id: annotation2329997
version: 1
top level: UV-WG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2329997/range2329997/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2329997/range2329997/1
type: Range
persistent identity: range2329997
display id: range2329997
version: 1
direction: direction/forward
top level: UV-WG
start: 201
end: 213
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2329996
display id: annotation2329996
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2329996/range2329996/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2329996/range2329996/1
type: Range
persistent identity: range2329996
display id: range2329996
version: 1
direction: direction/forward
top level: UV-WG
start: 201
end: 213
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation2330001
display id: annotation2330001
version: 1
top level: UV-WG
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330001/range2330001/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330001/range2330001/1
type: Range
persistent identity: range2330001
display id: range2330001
version: 1
direction: direction/reverse
top level: UV-WG
start: 220
end: 1293
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2330007
display id: annotation2330007
version: 1
top level: UV-WG
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330007/range2330007/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330007/range2330007/1
type: Range
persistent identity: range2330007
display id: range2330007
version: 1
direction: direction/forward
top level: UV-WG
start: 1417
end: 1430
orientation: inline

CDS
type: SequenceAnnotation
persistent identity: annotation2329999
display id: annotation2329999
version: 1
top level: UV-WG
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2329999/range2329999/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2329999/range2329999/1
type: Range
persistent identity: range2329999
display id: range2329999
version: 1
direction: direction/forward
top level: UV-WG
start: 220
end: 1293
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2330002
display id: annotation2330002
version: 1
top level: UV-WG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K994000/annotation2330002/range2330002/1

https://synbiohub.org/public/igem/BBa_K994000/annotation2330002/range2330002/1
type: Range
persistent identity: range2330002
display id: range2330002
version: 1
direction: direction/forward
top level: UV-WG
start: 1290
end: 1293
orientation: inline

B-carotene
type: ComponentDefinition
persistent identity: BBa K343006
display id: BBa_K343006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expresses B-carotene monooxygenase on a constitutive promotor
creator: Christian Kurtzhals
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction: beta-carotene + O2 = 2 retinal
mutable notes: The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
mutable provenance: The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K343001
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_K343001
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K343006_sequence/1

BBa_K343001
type: Component
persistent identity: component2085988
display id: component2085988
version: 1
top level: B-carotene
access: public
definition: ninaB

BBa_B0010
type: Component
persistent identity: component2085989
display id: component2085989
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2085991
display id: component2085991
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2085984
display id: component2085984
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2085978
display id: component2085978
version: 1
top level: B-carotene
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085989
display id: annotation2085989
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K343006/annotation2085989/range2085989/1
type: Range
persistent identity: range2085989
display id: range2085989
version: 1
direction: direction/forward
top level: B-carotene
start: 1952
end: 2031
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2085988
display id: annotation2085988
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343006/annotation2085988/range2085988/1
type: Range
persistent identity: range2085988
display id: range2085988
version: 1
direction: direction/reverse
top level: B-carotene
start: 81
end: 1943
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085984
display id: annotation2085984
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343006/annotation2085984/range2085984/1
type: Range
persistent identity: range2085984
display id: range2085984
version: 1
direction: direction/reverse
top level: B-carotene
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2085978
display id: annotation2085978
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343006/annotation2085978/range2085978/1
type: Range
persistent identity: range2085978
display id: range2085978
version: 1
direction: direction/forward
top level: B-carotene
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085991
display id: annotation2085991
version: 1
top level: B-carotene
location: https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K343006/annotation2085991/range2085991/1
type: Range
persistent identity: range2085991
display id: range2085991
version: 1
direction: direction/forward
top level: B-carotene
start: 2040
end: 2080
orientation: inline

BBa_K394003
type: ComponentDefinition
persistent identity: BBa K394003
display id: BBa_K394003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sex-Lethal from Drosophila melanogaster
creator: Brian Landry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:21Z
discontinued: false
dominant: false
group u list: _504_
m user id: 0
owner id: 6327
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the coding region for the Sex-Lethal Gene from which can be used to control splicing events.
mutable notes: none
mutable provenance: This was part was made using PCR from a construct synthesized by Mr. Gene. It intons have been artificially removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Sxl gene
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K394003_sequence/1

Sxl gene
type: SequenceAnnotation
persistent identity: annotation2102751
display id: annotation2102751
version: 1
top level: BBa_K394003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K394003/annotation2102751/range2102751/1

https://synbiohub.org/public/igem/BBa_K394003/annotation2102751/range2102751/1
type: Range
persistent identity: range2102751
display id: range2102751
version: 1
direction: direction/forward
top level: BBa_K394003
start: 1
end: 1062
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2095519
display id: annotation2095519
version: 1
top level: BBa_K394003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K394003/annotation2095519/range2095519/1

https://synbiohub.org/public/igem/BBa_K394003/annotation2095519/range2095519/1
type: Range
persistent identity: range2095519
display id: range2095519
version: 1
direction: direction/forward
top level: BBa_K394003
start: 1
end: 3
orientation: inline

metK
type: ComponentDefinition
persistent identity: BBa K1668001
display id: BBa_K1668001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metK (from Streptomyces avermitilis, increasing avermectin production)
creator: iGEM15_ZJU-China
created: 2015-08-13T11:00:00Z
modified: 2015-09-13T01:40:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2086_
m user id: 24510
owner id: 24510
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: coding sequence
mutable notes: overlap
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: stop coden
sequence annotation: start coden
sequence: https://synbiohub.org/public/igem/BBa_K1668001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2456726
display id: annotation2456726
version: 1
top level: metK
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1
type: Range
persistent identity: range2456726
display id: range2456726
version: 1
direction: direction/forward
top level: metK
start: 1
end: 816
orientation: inline

stop coden
type: SequenceAnnotation
persistent identity: annotation2447011
display id: annotation2447011
version: 1
top level: metK
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1
type: Range
persistent identity: range2447011
display id: range2447011
version: 1
direction: direction/forward
top level: metK
start: 811
end: 816
orientation: inline

start coden
type: SequenceAnnotation
persistent identity: annotation2447010
display id: annotation2447010
version: 1
top level: metK
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1
type: Range
persistent identity: range2447010
display id: range2447010
version: 1
direction: direction/forward
top level: metK
start: 1
end: 3
orientation: inline

BBa_K1067004
type: ComponentDefinition
persistent identity: BBa K1067004
display id: BBa_K1067004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hydroxylamine oxidoreductase
creator: Kristian Davidsen and Helen Cook
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ffff
mutable notes: fff
mutable provenance: fff
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: HAO
sequence annotation: Putative protein in samme operon
sequence: https://synbiohub.org/public/igem/BBa_K1067004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2359530
display id: annotation2359530
version: 1
top level: BBa_K1067004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359530/range2359530/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359530/range2359530/1
type: Range
persistent identity: range2359530
display id: range2359530
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 2817
end: 2819
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359529
display id: annotation2359529
version: 1
top level: BBa_K1067004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359529/range2359529/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359529/range2359529/1
type: Range
persistent identity: range2359529
display id: range2359529
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1710
end: 1712
orientation: inline

HAO
type: SequenceAnnotation
persistent identity: annotation2359512
display id: annotation2359512
version: 1
top level: BBa_K1067004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359512/range2359512/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359512/range2359512/1
type: Range
persistent identity: range2359512
display id: range2359512
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1
end: 1713
orientation: inline

Putative protein in samme operon
type: SequenceAnnotation
persistent identity: annotation2359526
display id: annotation2359526
version: 1
top level: BBa_K1067004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359526/range2359526/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359526/range2359526/1
type: Range
persistent identity: range2359526
display id: range2359526
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1710
end: 2819
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359528
display id: annotation2359528
version: 1
top level: BBa_K1067004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359528/range2359528/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359528/range2359528/1
type: Range
persistent identity: range2359528
display id: range2359528
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1711
end: 1713
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359527
display id: annotation2359527
version: 1
top level: BBa_K1067004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359527/range2359527/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359527/range2359527/1
type: Range
persistent identity: range2359527
display id: range2359527
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1
end: 3
orientation: inline

BBa_K1152008
type: ComponentDefinition
persistent identity: BBa K1152008
display id: BBa_K1152008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IndC Indigoidine Synthetase (cds)
creator: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1464_
m user id: 0
owner id: 16405
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The IndC Indigoidine Synthetase from Photorhabdus luminescens laumondii TT01 is a nonribosomal peptide synthetase (NRPS) which produces the blue pigment indigoidine. Indigoidine itself consists of two cyclic glutamines. IndC is a single module NRPS and consists of an Adenylation-, Thiolation- and a Thioesterase-domain. The A-domain thus contains an internal Oxidation domain.
mutable notes: The native indC contains an internal EcoRI and SpeI cutting site which was mutated using a CPEC approach.
mutable provenance: The coding sequence was amplified from Photorhabdus luminescens laumondii TT01 DSM15139.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: indC indigoidine synthetase (cds)
sequence annotation: silent mutation t-to-g
sequence annotation: silent mutation a-to-g
sequence: https://synbiohub.org/public/igem/BBa_K1152008_sequence/1

indC indigoidine synthetase (cds)
type: SequenceAnnotation
persistent identity: annotation2369272
display id: annotation2369272
version: 1
top level: BBa_K1152008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369272/range2369272/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369272/range2369272/1
type: Range
persistent identity: range2369272
display id: range2369272
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 1
end: 3855
orientation: inline

silent mutation t-to-g
type: SequenceAnnotation
persistent identity: annotation2369456
display id: annotation2369456
version: 1
top level: BBa_K1152008
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369456/range2369456/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369456/range2369456/1
type: Range
persistent identity: range2369456
display id: range2369456
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 513
end: 513
orientation: inline

silent mutation a-to-g
type: SequenceAnnotation
persistent identity: annotation2369455
display id: annotation2369455
version: 1
top level: BBa_K1152008
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1152008/annotation2369455/range2369455/1

https://synbiohub.org/public/igem/BBa_K1152008/annotation2369455/range2369455/1
type: Range
persistent identity: range2369455
display id: range2369455
version: 1
direction: direction/forward
top level: BBa_K1152008
start: 212
end: 212
orientation: inline

BBa_K1497011
type: ComponentDefinition
persistent identity: BBa K1497011
display id: BBa_K1497011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-ANS
creator: Sascha Hein, Sven Jager, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497011_sequence/1

BBa_M36004
type: ComponentDefinition
persistent identity: BBa M36004
display id: BBa_M36004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: predicted glycogen synthesis protein (w/ UTR&term)
creator: Jesse Palmer, Elliot Lui
created: 2011-04-27T11:00:00Z
modified: 2015-05-08T01:14:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9641
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for a protein involved in the biosynthesis of glycogen. Overproduction stimulates glycogen synthesis. Influences the first two enzymes in the e coli glycogen biosynthesis pathway (glucose-1-phosphate adenylyltransferase, glycogen synthase). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-REG-SUMMARY&object=EG11381 for more information.
mutable provenance: Genomic sequence for glgS as provided on EcoCyc database.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_M36009
component: BBa_M36010
component: BBa_M36008
sequence annotation: BBa_M36009
sequence annotation: BBa_M36008
sequence annotation: BBa_M36010
sequence: https://synbiohub.org/public/igem/BBa_M36004_sequence/1

BBa_M36009
type: Component
persistent identity: component2117837
display id: component2117837
version: 1
top level: BBa_M36004
access: public
definition: https://synbiohub.org/public/igem/BBa_M36009/1

BBa_M36010
type: Component
persistent identity: component2117839
display id: component2117839
version: 1
top level: BBa_M36004
access: public
definition: https://synbiohub.org/public/igem/BBa_M36010/1

BBa_M36008
type: Component
persistent identity: component2117838
display id: component2117838
version: 1
top level: BBa_M36004
access: public
definition: BBa_M36008

BBa_M36009
type: SequenceAnnotation
persistent identity: annotation2117837
display id: annotation2117837
version: 1
top level: BBa_M36004
location: https://synbiohub.org/public/igem/BBa_M36004/annotation2117837/range2117837/1
component: BBa_M36009

https://synbiohub.org/public/igem/BBa_M36004/annotation2117837/range2117837/1
type: Range
persistent identity: range2117837
display id: range2117837
version: 1
direction: direction/reverse
top level: BBa_M36004
start: 1
end: 47
orientation: inline

BBa_M36008
type: SequenceAnnotation
persistent identity: annotation2117838
display id: annotation2117838
version: 1
top level: BBa_M36004
location: https://synbiohub.org/public/igem/BBa_M36004/annotation2117838/range2117838/1
component: BBa_M36008

https://synbiohub.org/public/igem/BBa_M36004/annotation2117838/range2117838/1
type: Range
persistent identity: range2117838
display id: range2117838
version: 1
direction: direction/reverse
top level: BBa_M36004
start: 54
end: 254
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2117839
display id: annotation2117839
version: 1
top level: BBa_M36004
location: https://synbiohub.org/public/igem/BBa_M36004/annotation2117839/range2117839/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36004/annotation2117839/range2117839/1
type: Range
persistent identity: range2117839
display id: range2117839
version: 1
direction: direction/reverse
top level: BBa_M36004
start: 263
end: 344
orientation: inline

BBa_K1497023
type: ComponentDefinition
persistent identity: BBa K1497023
display id: BBa_K1497023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon B0034-F3H-B0034-DFR-B0034-eANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen, Sven Jager
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497012
component: BBa_K1497002
component: BBa_K1497013
sequence annotation: BBa_K1497002
sequence annotation: BBa_K1497013
sequence annotation: BBa_K1497012
sequence: https://synbiohub.org/public/igem/BBa_K1497023_sequence/1

BBa_K1497012
type: Component
persistent identity: component2399032
display id: component2399032
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497012

BBa_K1497002
type: Component
persistent identity: component2399037
display id: component2399037
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497002

BBa_K1497013
type: Component
persistent identity: component2399036
display id: component2399036
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497013

BBa_K1497002
type: SequenceAnnotation
persistent identity: annotation2399037
display id: annotation2399037
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399037/range2399037/1
component: BBa_K1497002

https://synbiohub.org/public/igem/BBa_K1497023/annotation2399037/range2399037/1
type: Range
persistent identity: range2399037
display id: range2399037
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 2222
end: 3189
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2399036
display id: annotation2399036
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399036/range2399036/1
component: BBa_K1497013

https://synbiohub.org/public/igem/BBa_K1497023/annotation2399036/range2399036/1
type: Range
persistent identity: range2399036
display id: range2399036
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 1134
end: 2213
orientation: inline

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2399032
display id: annotation2399032
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399032/range2399032/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497023/annotation2399032/range2399032/1
type: Range
persistent identity: range2399032
display id: range2399032
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 1
end: 1125
orientation: inline

BBa_K801060
type: ComponentDefinition
persistent identity: BBa K801060
display id: BBa_K801060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.
creator: Lara Kuntz
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11509
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part contains the (+)-Limonene Synthase 1 of ''Citrus limon''. It is preceeded by the yeast consensus sequence for improved expression and carries a C-Terminal Strep-Tag for purification or detection by westernblot. It is an improved version of BBa_I742111.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/21435702
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: Strep tag
sequence annotation: Coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K801060_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2197169
display id: annotation2197169
version: 1
top level: BBa_K801060
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197169/range2197169/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197169/range2197169/1
type: Range
persistent identity: range2197169
display id: range2197169
version: 1
direction: direction/forward
top level: BBa_K801060
start: 1
end: 7
orientation: inline

Strep tag
type: SequenceAnnotation
persistent identity: annotation2197211
display id: annotation2197211
version: 1
top level: BBa_K801060
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197211/range2197211/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197211/range2197211/1
type: Range
persistent identity: range2197211
display id: range2197211
version: 1
direction: direction/forward
top level: BBa_K801060
start: 1682
end: 1705
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2197213
display id: annotation2197213
version: 1
top level: BBa_K801060
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801060/annotation2197213/range2197213/1

https://synbiohub.org/public/igem/BBa_K801060/annotation2197213/range2197213/1
type: Range
persistent identity: range2197213
display id: range2197213
version: 1
direction: direction/forward
top level: BBa_K801060
start: 8
end: 1672
orientation: inline

BBa_K2042013
type: ComponentDefinition
persistent identity: BBa K2042013
display id: BBa_K2042013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS +PhaC_resinovorans + RBS + Pct
creator: Delphine Dessaux
created: 2016-09-02T11:00:00Z
modified: 2016-10-10T09:16:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2509_
m user id: 31392
owner id: 31381
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2042017
component: BBa_K2042010
component: BBa_K2042011
sequence annotation: BBa_K2042017
sequence annotation: BBa_K2042010
sequence annotation: BBa_K2042011
sequence: https://synbiohub.org/public/igem/BBa_K2042013_sequence/1

BBa_K2042017
type: Component
persistent identity: component2482858
display id: component2482858
version: 1
top level: BBa_K2042013
access: public
definition: https://synbiohub.org/public/igem/BBa_K2042017/1

BBa_K2042010
type: Component
persistent identity: component2482862
display id: component2482862
version: 1
top level: BBa_K2042013
access: public
definition: BBa_K2042010

BBa_K2042011
type: Component
persistent identity: component2482857
display id: component2482857
version: 1
top level: BBa_K2042013
access: public
definition: BBa_K2042011

BBa_K2042017
type: SequenceAnnotation
persistent identity: annotation2482858
display id: annotation2482858
version: 1
top level: BBa_K2042013
location: https://synbiohub.org/public/igem/BBa_K2042013/annotation2482858/range2482858/1
component: BBa_K2042017

https://synbiohub.org/public/igem/BBa_K2042013/annotation2482858/range2482858/1
type: Range
persistent identity: range2482858
display id: range2482858
version: 1
direction: direction/reverse
top level: BBa_K2042013
start: 1694
end: 1698
orientation: inline

BBa_K2042010
type: SequenceAnnotation
persistent identity: annotation2482862
display id: annotation2482862
version: 1
top level: BBa_K2042013
location: https://synbiohub.org/public/igem/BBa_K2042013/annotation2482862/range2482862/1
component: BBa_K2042010

https://synbiohub.org/public/igem/BBa_K2042013/annotation2482862/range2482862/1
type: Range
persistent identity: range2482862
display id: range2482862
version: 1
direction: direction/reverse
top level: BBa_K2042013
start: 1699
end: 3286
orientation: inline

BBa_K2042011
type: SequenceAnnotation
persistent identity: annotation2482857
display id: annotation2482857
version: 1
top level: BBa_K2042013
location: https://synbiohub.org/public/igem/BBa_K2042013/annotation2482857/range2482857/1
component: BBa_K2042011

https://synbiohub.org/public/igem/BBa_K2042013/annotation2482857/range2482857/1
type: Range
persistent identity: range2482857
display id: range2482857
version: 1
direction: direction/reverse
top level: BBa_K2042013
start: 1
end: 1693
orientation: inline

BBa_K1462040
type: ComponentDefinition
persistent identity: BBa K1462040
display id: BBa_K1462040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Crt
creator: Chuyao Fan
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:34Z
discontinued: false
dominant: false
group u list: _1841_
m user id: 0
owner id: 21369
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 1
mutable notes: 1`
mutable provenance: 11
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1462040_sequence/1

BBa_K801097
type: ComponentDefinition
persistent identity: BBa K801097
display id: BBa_K801097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 13213
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Chalcone O-methyltransferase (OMT1) coding region from ''Humulus lupulus'' preceeded by yeast consensus sequence for improved expression.
mutable notes: -
mutable provenance: http://www.ncbi.nlm.nih.gov/nucleotide/167613934/
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Consensus sequence
sequence annotation: OMT
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K801097_sequence/1

Consensus sequence
type: SequenceAnnotation
persistent identity: annotation2202171
display id: annotation2202171
version: 1
top level: BBa_K801097
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202171/range2202171/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202171/range2202171/1
type: Range
persistent identity: range2202171
display id: range2202171
version: 1
direction: direction/forward
top level: BBa_K801097
start: 1
end: 6
orientation: inline

OMT
type: SequenceAnnotation
persistent identity: annotation2202172
display id: annotation2202172
version: 1
top level: BBa_K801097
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202172/range2202172/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202172/range2202172/1
type: Range
persistent identity: range2202172
display id: range2202172
version: 1
direction: direction/forward
top level: BBa_K801097
start: 7
end: 1062
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2202170
display id: annotation2202170
version: 1
top level: BBa_K801097
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801097/annotation2202170/range2202170/1

https://synbiohub.org/public/igem/BBa_K801097/annotation2202170/range2202170/1
type: Range
persistent identity: range2202170
display id: range2202170
version: 1
direction: direction/forward
top level: BBa_K801097
start: 7
end: 9
orientation: inline

gldA
type: ComponentDefinition
persistent identity: BBa K1172201
display id: BBa_K1172201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GldA - glycerol dehydrogenase from Escherichia coli
creator: Lukas Rositzka
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Gene gldA encodes glycerol dehydrogenase from Escherichicha coli. The overexpression of glycerol dehydrogenase in E. coli can be used as a way for endogenous mediator production. Many derivates of glycerol dehydrogenase are small, water-soluble redoxmolecules, which have mediator properties. In enzymology, a glycerol dehydrogenase (EC 1.1.1.6) is an enzyme belonging to the family of oxireductases that catalyzes the chemical reaction: Glycerol + NAD --> Glycerone + NADH + H
mutable notes: Primeroverlap for Gibson-Assembly
mutable provenance: Escherichicha coli KRX genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: gldA
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1172201_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2370297
display id: annotation2370297
version: 1
top level: gldA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370297/range2370297/1
type: Range
persistent identity: range2370297
display id: range2370297
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 3
orientation: inline

gldA
type: SequenceAnnotation
persistent identity: annotation2332911
display id: annotation2332911
version: 1
top level: gldA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2332911/range2332911/1
type: Range
persistent identity: range2332911
display id: range2332911
version: 1
direction: direction/forward
top level: gldA
start: 1
end: 1104
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2370298
display id: annotation2370298
version: 1
top level: gldA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1

https://synbiohub.org/public/igem/BBa_K1172201/annotation2370298/range2370298/1
type: Range
persistent identity: range2370298
display id: range2370298
version: 1
direction: direction/forward
top level: gldA
start: 1102
end: 1104
orientation: inline

LipA
type: ComponentDefinition
persistent identity: BBa K836000
display id: BBa_K836000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lipA from B. cepacia (codon usage optimized for E. coli)
creator: Ricardo Alvarado
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol. It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit).
mutable notes: The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.
mutable provenance: The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lipA
sequence: https://synbiohub.org/public/igem/BBa_K836000_sequence/1

lipA
type: SequenceAnnotation
persistent identity: annotation2185259
display id: annotation2185259
version: 1
top level: LipA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K836000/annotation2185259/range2185259/1

https://synbiohub.org/public/igem/BBa_K836000/annotation2185259/range2185259/1
type: Range
persistent identity: range2185259
display id: range2185259
version: 1
direction: direction/forward
top level: LipA
start: 1
end: 1098
orientation: inline

cry11Aa
type: ComponentDefinition
persistent identity: BBa K332011
display id: BBa_K332011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.
creator: Chia-Le Meng
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7487
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. The cry11Aa gene is cloned from Bacillus thuringiensis subsp. Israelensis. The cry protein is controlled by the tetR-repressible promoter PtetR (BBa_R0040), which in turn is regulated by a temperature control system. When E.coli is released into environment (<37C), tetR begins to degrade, resulting in the promoter PtetR (BBa_R0011) expressing downstream genes, cry11Aa (BBa_K332012) and Green Fluorescent Protein (GFP)(BBa_E0040).
mutable notes: Procedures (I) Culture Bti. Bacillus thuringiensis subsp. Israelensis (BCRC15860) 1. Prepare agar plate for Bti. Beef extract 3.0g Peptone 5.0g Agar 15.0g ddH2O 1 L *adjust PH to 7.0 *No antibiotic *Be aware of contamination 2. Plate Bti. on the medium and incubate the cultures at 30??C overnight. (II) Clone cry11Aa from Bti. into TA vector 1. Extract genomic DNA of Bti. by liquid nitrogen. 2. Add some ddH2O to dilute the DNA. 3. Design primers Vf2: ATTCAATAAAAGGTGGAATGAATTATGGA Tm: 53??C VR: GTGCTAACATGACTTCTACTTTAGT Tm: 52.8??C 4. Find the best PCR condition by gradient PCR. Anneling temperature: 51??C??10??C 5. PCR by B-taq plus on the best condition Template DNA（10ng/μl） 2.0 B-taq buffer 5.0 dNTP（2mM） 5.0 forward primer（10μM） 1.5 reverse primer（10μM） 1.5 B-taq plus DNA polymerase（2Kb） 1.0 ddH2O 34.0 Total 50 μl 6. Digestion to confirm the cry11Aa fragment and enzyme sites. 7. TA clone 8. DNA sequencing (III) PCR mutagenesis at two enzyme sites --- EcoR1 and Spe1 1. Design primers by primerX. EcoR1-Vf2: CGG GTA CAA TCT CAG AAC TCG GGA AAT AAT AGA A EcoR1-VR: TTC TAT TAT TTC CCG AGT TCT GAG ATT GTA CCC G Spe1-Vf2: ATA ATG AAT GGG GAG GAC TGG TTT ATA AGT TAT TAA TGG G Spe1-VR: CTT CCC CCA TTA ATA ACT TAT AAA CTA GTC CTC CCC ATT CAT 2. Digestion to confirm the fragments (IV) PCR construction of Biobrick parts 1. Design primers by Assembly standard 10. Vf2: GTTTCTTC GAATTC GCGGCCGC T TCTAG ATGGAAGATAGTTCTTTAGATACT VR: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTACTTTAGTAACGGATT 2. PCR condition 3. Ligation to backbones(Psb1C3). (V) Transform into E.coli 1. Thaw competent cells and BBa_K332012 plasmid on ice. 2. Add 2ul plasmid to competent cell and place in ice for 5 minutes. 3. Put the transformed cells into 42℃ water bath for 45 seconds. 4. Plate the cells on the appropriate media and antibiotic, such as agar plates with 25 ??g/ml kanamycin. 5. Incubate the cultures at 37??C overnight. (VI) Culture with mosquito larvae and observe
mutable provenance: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis. BCRC Number: 15860 Organism: Bacillus thuringiensis subsp. israelensis de Barjac History: << ATCC << USDA << ONR 60 << H. de Barjac << L. J. Goldberg 60 Other Collection No.: ATCC 35646 Source: sewage, Israel Characterization: Production of delta-endotoxin against dipteran larvae --B3009 Others: referred to as the type strain --B3008 Growth Conditions: 30??C , Medium ID: 3 Oxygen Requirement: Aerobic Biosafety Level: 1 References: Temeyer, K. B. 1984. Larvicidal activity of Bacillus thuringiensis subsp. israelensis in the dipteran Haematobia irritans. Appl. Environ. Microbiol.47 : 952-955. PMID: 6742837. Demezas, D. H. and J. Bell. 1995. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. Syst. Appl. Microbiol.18 : 582-589.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K332011_sequence/1

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet5
sequence annotation: Sec signal
sequence annotation: Beta sheet1
sequence annotation: CsgG signal
sequence annotation: mutation
sequence annotation: Beta sheet4
sequence annotation: CsgA-WT
sequence annotation: Beta sheet3
sequence annotation: Beta sheet2
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

BBa_K1497002
type: ComponentDefinition
persistent identity: BBa K1497002
display id: BBa_K1497002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-eANS (engineered anthocyanidin synthase with strong RBS)
creator: Sascha Hein, Sven Jager, Thomas Dohmen, Sven Rumpf
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: bablabla e. coli optimiert etcc...
mutable notes: Gene was E. coli optimated synthezied by Eurofins.
mutable provenance: Fragia anassana
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497002_sequence/1

BBa_K1331005
type: ComponentDefinition
persistent identity: BBa K1331005
display id: BBa_K1331005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlABRI operon genes from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1331001
component: BBa_K1331002
component: BBa_K1331004
component: BBa_K1331003
sequence annotation: BBa_K1331001
sequence annotation: BBa_K1331002
sequence annotation: BBa_K1331003
sequence annotation: BBa_K1331004
sequence: https://synbiohub.org/public/igem/BBa_K1331005_sequence/1

BBa_K1331001
type: Component
persistent identity: component2421124
display id: component2421124
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331001

BBa_K1331002
type: Component
persistent identity: component2421128
display id: component2421128
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331002

BBa_K1331004
type: Component
persistent identity: component2421125
display id: component2421125
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331004

BBa_K1331003
type: Component
persistent identity: component2421130
display id: component2421130
version: 1
top level: BBa_K1331005
access: public
definition: BBa_K1331003

BBa_K1331001
type: SequenceAnnotation
persistent identity: annotation2421124
display id: annotation2421124
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421124/range2421124/1
component: BBa_K1331001

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421124/range2421124/1
type: Range
persistent identity: range2421124
display id: range2421124
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 1
end: 947
orientation: inline

BBa_K1331002
type: SequenceAnnotation
persistent identity: annotation2421128
display id: annotation2421128
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421128/range2421128/1
component: BBa_K1331002

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421128/range2421128/1
type: Range
persistent identity: range2421128
display id: range2421128
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 2271
end: 3167
orientation: inline

BBa_K1331003
type: SequenceAnnotation
persistent identity: annotation2421130
display id: annotation2421130
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421130/range2421130/1
component: BBa_K1331003

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421130/range2421130/1
type: Range
persistent identity: range2421130
display id: range2421130
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 3176
end: 3894
orientation: inline

BBa_K1331004
type: SequenceAnnotation
persistent identity: annotation2421125
display id: annotation2421125
version: 1
top level: BBa_K1331005
location: https://synbiohub.org/public/igem/BBa_K1331005/annotation2421125/range2421125/1
component: BBa_K1331004

https://synbiohub.org/public/igem/BBa_K1331005/annotation2421125/range2421125/1
type: Range
persistent identity: range2421125
display id: range2421125
version: 1
direction: direction/reverse
top level: BBa_K1331005
start: 956
end: 2262
orientation: inline

BBa_K1660009
type: ComponentDefinition
persistent identity: BBa K1660009
display id: BBa_K1660009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-B0034-rMpL
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T01:13:59Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: encodes a nematotoxic lectin from the parasol mushroom(Macrolepiota procera) under the control of a constitutive promoter.
mutable notes: We optimized the codon sequence for E. coli. and add a constitutive promoter J23100 upstream the coding sequence of rMpL, so that this parts can express rMpL lectin continuously.
mutable provenance: It comes from Macrolepiota procera cDNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop codon
sequence annotation: B0034
sequence annotation: J23100
sequence annotation: rMpL
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1660009_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2446551
display id: annotation2446551
version: 1
top level: BBa_K1660009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660009/annotation2446551/range2446551/1

https://synbiohub.org/public/igem/BBa_K1660009/annotation2446551/range2446551/1
type: Range
persistent identity: range2446551
display id: range2446551
version: 1
direction: direction/forward
top level: BBa_K1660009
start: 485
end: 487
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2446548
display id: annotation2446548
version: 1
top level: BBa_K1660009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660009/annotation2446548/range2446548/1

https://synbiohub.org/public/igem/BBa_K1660009/annotation2446548/range2446548/1
type: Range
persistent identity: range2446548
display id: range2446548
version: 1
direction: direction/forward
top level: BBa_K1660009
start: 44
end: 55
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2446547
display id: annotation2446547
version: 1
top level: BBa_K1660009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660009/annotation2446547/range2446547/1

https://synbiohub.org/public/igem/BBa_K1660009/annotation2446547/range2446547/1
type: Range
persistent identity: range2446547
display id: range2446547
version: 1
direction: direction/forward
top level: BBa_K1660009
start: 1
end: 35
orientation: inline

rMpL
type: SequenceAnnotation
persistent identity: annotation2446549
display id: annotation2446549
version: 1
top level: BBa_K1660009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660009/annotation2446549/range2446549/1

https://synbiohub.org/public/igem/BBa_K1660009/annotation2446549/range2446549/1
type: Range
persistent identity: range2446549
display id: range2446549
version: 1
direction: direction/forward
top level: BBa_K1660009
start: 62
end: 487
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2446550
display id: annotation2446550
version: 1
top level: BBa_K1660009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660009/annotation2446550/range2446550/1

https://synbiohub.org/public/igem/BBa_K1660009/annotation2446550/range2446550/1
type: Range
persistent identity: range2446550
display id: range2446550
version: 1
direction: direction/forward
top level: BBa_K1660009
start: 62
end: 64
orientation: inline

BBa_K1554003
type: ComponentDefinition
persistent identity: BBa K1554003
display id: BBa_K1554003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EaDAcT
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:29:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: diacylglycerol acetyltransferase
mutable notes: BioBrick compatible
mutable provenance: It comes from Euonymus alatus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554003_sequence/1

ninaB
type: ComponentDefinition
persistent identity: BBa K343001
display id: BBa_K343001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.
creator: Christian Kurtzhals
created: 2010-07-01T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This coding region encodes a beta-carotene deoxygenase, that splits beta-carotene into two retinal molecules.
mutable notes: No considerations have been made so far concerning design
mutable provenance: Sequence taken from cDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Coding sequence
sequence annotation: met
sequence: https://synbiohub.org/public/igem/BBa_K343001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2072321
display id: annotation2072321
version: 1
top level: ninaB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072321/range2072321/1
type: Range
persistent identity: range2072321
display id: range2072321
version: 1
direction: direction/forward
top level: ninaB
start: 1861
end: 1863
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2072319
display id: annotation2072319
version: 1
top level: ninaB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072319/range2072319/1
type: Range
persistent identity: range2072319
display id: range2072319
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 1860
orientation: inline

met
type: SequenceAnnotation
persistent identity: annotation2072320
display id: annotation2072320
version: 1
top level: ninaB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1

https://synbiohub.org/public/igem/BBa_K343001/annotation2072320/range2072320/1
type: Range
persistent identity: range2072320
display id: range2072320
version: 1
direction: direction/forward
top level: ninaB
start: 1
end: 3
orientation: inline