##gff-version 3
##sequence-region BBa_S04187 1 914
BBa_S04187	.	promoter	1	45	.	+	0	ID=BBa_R2000;Name=BBa_R2000
BBa_S04187	.	promoter	33	38	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_S04187	.	promoter	10	15	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_S04187	.	start_codon	45	45	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_S04187	.	non_covalent_binding_site	20	33	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_S04187	.	sequence_feature	1	9	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_S04187	.	engineered_region	54	914	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_S04187	.	CDS	72	752	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_S04187	.	engineered_tag	753	777	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04187	.	CDS	72	746	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04187	.	terminator	786	865	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_S04187	.	engineered_region	786	865	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04187	.	stem_loop	797	840	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04187	.	ribosome_entry_site	54	65	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_S04187	.	conserved_region	58	61	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04187	.	terminator	874	914	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_S04187	.	stop_codon	907	907	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04187	.	polyA_site	901	914	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04187	.	stem_loop	881	900	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04187	.	engineered_region	874	914	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S04187
agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatggcttc
ctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatc
gaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgt
tcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggacta
cctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc
caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccgg
ttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaat
caaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggtt
cagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt
acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata