##gff-version 3
##sequence-region BBa_S01300 1 4074
BBa_S01300	.	promoter	3093	3146	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01300	.	non_covalent_binding_site	3093	3111	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01300	.	promoter	3135	3140	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01300	.	promoter	3112	3117	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01300	.	engineered_region	3093	3146	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01300	.	non_covalent_binding_site	3118	3136	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01300	.	ribosome_entry_site	2165	2179	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01300	.	ribosome_entry_site	2172	2176	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01300	.	engineered_region	2165	2179	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01300	.	ribosome_entry_site	2165	2179	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01300	.	terminator	778	857	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01300	.	stem_loop	789	832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01300	.	engineered_region	778	857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01300	.	promoter	915	1114	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01300	.	non_covalent_binding_site	1003	1040	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01300	.	engineered_region	915	1114	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01300	.	non_covalent_binding_site	1080	1114	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01300	.	promoter	1075	1080	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01300	.	start_codon	1087	1087	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01300	.	CDS	915	1002	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01300	.	promoter	1051	1056	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01300	.	terminator	1966	2045	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01300	.	stem_loop	1977	2020	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01300	.	engineered_region	1966	2045	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01300	.	terminator	2054	2094	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01300	.	engineered_region	2054	2094	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01300	.	stem_loop	2061	2080	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01300	.	stop_codon	2087	2087	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01300	.	polyA_site	2081	2094	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01300	.	ribosome_entry_site	3155	3169	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01300	.	ribosome_entry_site	3162	3166	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01300	.	engineered_region	3155	3169	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01300	.	ribosome_entry_site	3155	3169	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01300	.	ribosome_entry_site	106	120	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01300	.	ribosome_entry_site	113	117	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01300	.	engineered_region	106	120	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01300	.	ribosome_entry_site	106	120	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01300	.	terminator	3044	3084	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01300	.	engineered_region	3044	3084	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01300	.	stem_loop	3051	3070	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01300	.	stop_codon	3077	3077	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01300	.	polyA_site	3071	3084	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01300	.	terminator	2956	3035	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01300	.	stem_loop	2967	3010	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01300	.	engineered_region	2956	3035	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01300	.	terminator	3946	4025	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01300	.	stem_loop	3957	4000	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01300	.	engineered_region	3946	4025	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01300	.	ribosome_entry_site	1123	1137	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01300	.	ribosome_entry_site	1130	1134	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01300	.	engineered_region	1123	1137	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01300	.	ribosome_entry_site	1123	1137	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01300	.	CDS	127	744	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01300	.	CDS	127	705	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01300	.	tag	706	737	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01300	.	engineered_tag	745	769	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01300	.	engineered_region	127	744	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01300	.	terminator	866	906	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01300	.	engineered_region	866	906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01300	.	stem_loop	873	892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01300	.	stop_codon	899	899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01300	.	polyA_site	893	906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01300	.	engineered_region	2186	2947	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01300	.	CDS	2186	2947	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01300	.	tag	2904	2941	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01300	.	engineered_region	2186	2947	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01300	.	point_mutation	2254	2254	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01300	.	stop_codon	2942	2947	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01300	.	CDS	1144	1932	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01300	.	engineered_region	1144	1932	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01300	.	engineered_tag	1933	1957	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01300	.	start_codon	1144	1146	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01300	.	tag	1894	1926	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01300	.	stop_codon	1927	1932	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01300	.	CDS	1144	1893	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01300	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01300	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01300	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01300	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01300	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01300	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01300	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01300	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01300	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01300	.	promoter	2103	2156	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01300	.	non_covalent_binding_site	2103	2121	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01300	.	promoter	2145	2150	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01300	.	promoter	2122	2127	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01300	.	engineered_region	2103	2156	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01300	.	non_covalent_binding_site	2128	2146	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01300	.	engineered_region	3176	3937	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01300	.	stop_codon	3932	3937	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01300	.	CDS	3176	3937	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01300	.	sequence_alteration	3417	3417	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01300	.	engineered_region	3176	3937	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01300	.	tag	3893	3931	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01300	.	terminator	4034	4074	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01300	.	engineered_region	4034	4074	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01300	.	stem_loop	4041	4060	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01300	.	stop_codon	4067	4067	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01300	.	polyA_site	4061	4074	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S01300
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggatttttt
ggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggactta
gttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaa
atgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtca
acagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataac
tctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaa
cagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattca
tgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgca
aacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggcc
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagattaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgt
atgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttgg
agactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtac
atttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgag
ccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacac
caatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaattt
aaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccaggg
catcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaatt
ttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgat
gaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagct
gcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatcccta
tcagtgatagagatactgagcactactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttc
accggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagg
gcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgt
gaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcc
atgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagt
tcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaa
gctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaag
atccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccg
tgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggt
cctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaa
aactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaa
gaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcg
acgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcat
ctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgc
taccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttct
tcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaa
gggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatc
atggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcg
ccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtc
cgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctc
ggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata