BBa_M36702
type: ComponentDefinition
persistent identity: BBa M36702
display id: BBa_M36702
version: 1
was generated by: iGEM to SBOL conversion
description: Pyruvate Sensor v2 w/ Gemini
created: 2011-05-05T11:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: See BBa_M36701
owned by: james
owned by: myers
mutable description: Similar to BBa_M36701, this is a coupling of the Pyruvate sensor (BBa_M36698) with the Gemini Protein Generator (BBa_M36709)
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A fix for BBa_M36701 which had a few design flaws.
star: false
creator: Wyatt Woodson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36709
component: BBa_M36698
sequence annotation: BBa_M36698
sequence annotation: BBa_M36709
sequence: https://synbiohub.org/public/igem/BBa_M36702_sequence/1

BBa_M36709
type: Component
persistent identity: component2119712
display id: component2119712
version: 1
top level: BBa_M36702
definition: BBa_M36709
access: public

BBa_M36698
type: Component
persistent identity: component2119702
display id: component2119702
version: 1
top level: BBa_M36702
definition: BBa_M36698
access: public

BBa_M36698
type: SequenceAnnotation
persistent identity: annotation2119702
display id: annotation2119702
version: 1
top level: BBa_M36702
location: https://synbiohub.org/public/igem/BBa_M36702/annotation2119702/range2119702/1
component: BBa_M36698

https://synbiohub.org/public/igem/BBa_M36702/annotation2119702/range2119702/1
type: Range
persistent identity: range2119702
display id: range2119702
version: 1
top level: BBa_M36702
direction: direction/reverse
start: 1
end: 1207
orientation: inline

BBa_M36709
type: SequenceAnnotation
persistent identity: annotation2119712
display id: annotation2119712
version: 1
top level: BBa_M36702
location: https://synbiohub.org/public/igem/BBa_M36702/annotation2119712/range2119712/1
component: BBa_M36709

https://synbiohub.org/public/igem/BBa_M36702/annotation2119712/range2119712/1
type: Range
persistent identity: range2119712
display id: range2119712
version: 1
top level: BBa_M36702
direction: direction/reverse
start: 1216
end: 2262
orientation: inline

BBa_M36709
type: ComponentDefinition
persistent identity: BBa M36709
display id: BBa_M36709
version: 1
was generated by: iGEM to SBOL conversion
description: Gemini Protein Generator
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al.
owned by: james
owned by: myers
mutable description: A medium strength RBS expressing Gemini, ending with a strong terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Test Actuator
star: false
creator: Jeff Quinn and Wyatt Woodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E0051
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_M36709_sequence/1

BBa_B0010
type: Component
persistent identity: component2118635
display id: component2118635
version: 1
top level: BBa_M36709
definition: BBa_B0010
access: public

BBa_E0051
type: Component
persistent identity: component2118634
display id: component2118634
version: 1
top level: BBa_M36709
definition: BBa_E0051
access: public

BBa_B0032
type: Component
persistent identity: component2118629
display id: component2118629
version: 1
top level: BBa_M36709
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2118629
display id: annotation2118629
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
type: Range
persistent identity: range2118629
display id: range2118629
version: 1
top level: BBa_M36709
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0051
type: SequenceAnnotation
persistent identity: annotation2118634
display id: annotation2118634
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
component: BBa_E0051

https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
type: Range
persistent identity: range2118634
display id: range2118634
version: 1
top level: BBa_M36709
direction: direction/reverse
start: 14
end: 967
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2118635
display id: annotation2118635
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
type: Range
persistent identity: range2118635
display id: range2118635
version: 1
top level: BBa_M36709
direction: direction/forward
start: 968
end: 1047
orientation: inline

BBa_M36698
type: ComponentDefinition
persistent identity: BBa M36698
display id: BBa_M36698
version: 1
was generated by: iGEM to SBOL conversion
description: Pyruvate Sensor v2
created: 2011-05-05T11:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: See BBa_M36708
owned by: james
owned by: myers
mutable description: Similar to BBa_M36708, this provides a way to test for pyruvate through IclR inhibition of PaceBAK (a portion of the aceBAK promoter).
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BBa_M36708 had a couple design flaws which have been fixed
star: false
creator: Wyatt Woodson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36699
component: BBa_M36010
component: BBa_M36706
component: BBa_M36001
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_M36001
sequence annotation: BBa_M36706
sequence annotation: BBa_M36010
sequence annotation: BBa_M36699
sequence: https://synbiohub.org/public/igem/BBa_M36698_sequence/1

BBa_M36699
type: Component
persistent identity: component2119680
display id: component2119680
version: 1
top level: BBa_M36698
definition: BBa_M36699
access: public

BBa_M36010
type: Component
persistent identity: component2119667
display id: component2119667
version: 1
top level: BBa_M36698
definition: BBa_M36010
access: public

BBa_M36706
type: Component
persistent identity: component2119666
display id: component2119666
version: 1
top level: BBa_M36698
definition: BBa_M36706
access: public

BBa_M36001
type: Component
persistent identity: component2119662
display id: component2119662
version: 1
top level: BBa_M36698
definition: BBa_M36001
access: public

BBa_J23119
type: Component
persistent identity: component2119661
display id: component2119661
version: 1
top level: BBa_M36698
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2119661
display id: annotation2119661
version: 1
top level: BBa_M36698
location: https://synbiohub.org/public/igem/BBa_M36698/annotation2119661/range2119661/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_M36698/annotation2119661/range2119661/1
type: Range
persistent identity: range2119661
display id: range2119661
version: 1
top level: BBa_M36698
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2119662
display id: annotation2119662
version: 1
top level: BBa_M36698
location: https://synbiohub.org/public/igem/BBa_M36698/annotation2119662/range2119662/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36698/annotation2119662/range2119662/1
type: Range
persistent identity: range2119662
display id: range2119662
version: 1
top level: BBa_M36698
direction: direction/reverse
start: 36
end: 82
orientation: inline

BBa_M36706
type: SequenceAnnotation
persistent identity: annotation2119666
display id: annotation2119666
version: 1
top level: BBa_M36698
location: https://synbiohub.org/public/igem/BBa_M36698/annotation2119666/range2119666/1
component: BBa_M36706

https://synbiohub.org/public/igem/BBa_M36698/annotation2119666/range2119666/1
type: Range
persistent identity: range2119666
display id: range2119666
version: 1
top level: BBa_M36698
direction: direction/reverse
start: 83
end: 907
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2119667
display id: annotation2119667
version: 1
top level: BBa_M36698
location: https://synbiohub.org/public/igem/BBa_M36698/annotation2119667/range2119667/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36698/annotation2119667/range2119667/1
type: Range
persistent identity: range2119667
display id: range2119667
version: 1
top level: BBa_M36698
direction: direction/reverse
start: 908
end: 989
orientation: inline

BBa_M36699
type: SequenceAnnotation
persistent identity: annotation2119680
display id: annotation2119680
version: 1
top level: BBa_M36698
location: https://synbiohub.org/public/igem/BBa_M36698/annotation2119680/range2119680/1
component: BBa_M36699

https://synbiohub.org/public/igem/BBa_M36698/annotation2119680/range2119680/1
type: Range
persistent identity: range2119680
display id: range2119680
version: 1
top level: BBa_M36698
direction: direction/reverse
start: 990
end: 1207
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_M36001
type: ComponentDefinition
persistent identity: BBa M36001
display id: BBa_M36001
version: 1
was generated by: iGEM to SBOL conversion
description: 5' Bicistronic UTR (medium), does not include ATG start
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Vivek Mutalik et al. c/o BIOFAB Emeryville.
owned by: james
owned by: myers
mutable description: 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Please see long description.
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M36001_sequence/1

BBa_M36010
type: ComponentDefinition
persistent identity: BBa M36010
display id: BBa_M36010
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Terminator (Strong)
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Guillaume Cambray et al. c/o BIOFAB Emeryville
owned by: james
owned by: myers
mutable description: E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
discontinued: false
dominant: true
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: please see long description
star: false
creator: Drew Endy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_M36010_sequence/1

BBa_M36706
type: ComponentDefinition
persistent identity: BBa M36706
display id: BBa_M36706
version: 1
was generated by: iGEM to SBOL conversion
description: Gene encoding IclR
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: This coding sequence was obtained from genome.jp
owned by: james
owned by: myers
mutable description: IclR represses the aceBAK promoter in E. coli
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Katie Lund and Wyatt Woodson
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: IclR Coding Region
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M36706_sequence/1

IclR Coding Region
type: SequenceAnnotation
persistent identity: annotation2119221
display id: annotation2119221
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119221/range2119221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M36706/annotation2119221/range2119221/1
type: Range
persistent identity: range2119221
display id: range2119221
version: 1
top level: BBa_M36706
direction: direction/forward
start: 1
end: 825
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2119104
display id: annotation2119104
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119104/range2119104/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M36706/annotation2119104/range2119104/1
type: Range
persistent identity: range2119104
display id: range2119104
version: 1
top level: BBa_M36706
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2119109
display id: annotation2119109
version: 1
top level: BBa_M36706
location: https://synbiohub.org/public/igem/BBa_M36706/annotation2119109/range2119109/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M36706/annotation2119109/range2119109/1
type: Range
persistent identity: range2119109
display id: range2119109
version: 1
top level: BBa_M36706
direction: direction/forward
start: 823
end: 825
orientation: inline

BBa_M36699
type: ComponentDefinition
persistent identity: BBa M36699
display id: BBa_M36699
version: 1
was generated by: iGEM to SBOL conversion
description: PaceBAK
created: 2011-05-05T11:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: See BBa_M36707
owned by: james
owned by: myers
mutable description: A portion of the aceBAK promoter region that comprises the promoter as well as the IclR binding sites.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is an updated version of BBa_M36707
star: false
creator: Wyatt Woodson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: IclR Box I
sequence annotation: IclR Box II
sequence annotation: IclR Box III
sequence annotation: IclR Box IV
sequence annotation: IHF Binding Site
sequence annotation: IclR Box V
sequence annotation: IclR Box VI
sequence annotation: CRP-cAMP Binding Site
sequence annotation: IclR Box VII
sequence annotation: -35 Region
sequence annotation: IclR Box VIII
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_M36699_sequence/1

IclR Box I
type: SequenceAnnotation
persistent identity: annotation2119606
display id: annotation2119606
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119606/range2119606/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36699/annotation2119606/range2119606/1
type: Range
persistent identity: range2119606
display id: range2119606
version: 1
top level: BBa_M36699
direction: direction/forward
start: 11
end: 17
orientation: inline

IclR Box II
type: SequenceAnnotation
persistent identity: annotation2119607
display id: annotation2119607
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119607/range2119607/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36699/annotation2119607/range2119607/1
type: Range
persistent identity: range2119607
display id: range2119607
version: 1
top level: BBa_M36699
direction: direction/forward
start: 21
end: 27
orientation: inline

IclR Box III
type: SequenceAnnotation
persistent identity: annotation2119608
display id: annotation2119608
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119608/range2119608/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36699/annotation2119608/range2119608/1
type: Range
persistent identity: range2119608
display id: range2119608
version: 1
top level: BBa_M36699
direction: direction/forward
start: 30
end: 36
orientation: inline

IclR Box IV
type: SequenceAnnotation
persistent identity: annotation2119609
display id: annotation2119609
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119609/range2119609/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119609/range2119609/1
type: Range
persistent identity: range2119609
display id: range2119609
version: 1
top level: BBa_M36699
direction: direction/forward
start: 48
end: 54
orientation: inline

IHF Binding Site
type: SequenceAnnotation
persistent identity: annotation2119611
display id: annotation2119611
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119611/range2119611/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119611/range2119611/1
type: Range
persistent identity: range2119611
display id: range2119611
version: 1
top level: BBa_M36699
direction: direction/forward
start: 76
end: 89
orientation: inline

IclR Box V
type: SequenceAnnotation
persistent identity: annotation2119610
display id: annotation2119610
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119610/range2119610/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119610/range2119610/1
type: Range
persistent identity: range2119610
display id: range2119610
version: 1
top level: BBa_M36699
direction: direction/forward
start: 99
end: 105
orientation: inline

IclR Box VI
type: SequenceAnnotation
persistent identity: annotation2119612
display id: annotation2119612
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119612/range2119612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119612/range2119612/1
type: Range
persistent identity: range2119612
display id: range2119612
version: 1
top level: BBa_M36699
direction: direction/forward
start: 110
end: 116
orientation: inline

CRP-cAMP Binding Site
type: SequenceAnnotation
persistent identity: annotation2119615
display id: annotation2119615
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119615/range2119615/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36699/annotation2119615/range2119615/1
type: Range
persistent identity: range2119615
display id: range2119615
version: 1
top level: BBa_M36699
direction: direction/forward
start: 114
end: 135
orientation: inline

IclR Box VII
type: SequenceAnnotation
persistent identity: annotation2119613
display id: annotation2119613
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119613/range2119613/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119613/range2119613/1
type: Range
persistent identity: range2119613
display id: range2119613
version: 1
top level: BBa_M36699
direction: direction/forward
start: 118
end: 124
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation2119616
display id: annotation2119616
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119616/range2119616/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M36699/annotation2119616/range2119616/1
type: Range
persistent identity: range2119616
display id: range2119616
version: 1
top level: BBa_M36699
direction: direction/forward
start: 119
end: 127
orientation: inline

IclR Box VIII
type: SequenceAnnotation
persistent identity: annotation2119614
display id: annotation2119614
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119614/range2119614/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_M36699/annotation2119614/range2119614/1
type: Range
persistent identity: range2119614
display id: range2119614
version: 1
top level: BBa_M36699
direction: direction/forward
start: 126
end: 131
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation2119617
display id: annotation2119617
version: 1
top level: BBa_M36699
location: https://synbiohub.org/public/igem/BBa_M36699/annotation2119617/range2119617/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M36699/annotation2119617/range2119617/1
type: Range
persistent identity: range2119617
display id: range2119617
version: 1
top level: BBa_M36699
direction: direction/forward
start: 143
end: 150
orientation: inline

BBa_E0051
type: ComponentDefinition
persistent identity: BBa E0051
display id: BBa_E0051
version: 1
was generated by: iGEM to SBOL conversion
description: lacZa.GFP fusion
created: 2009-03-01T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)
owned by: james
owned by: myers
mutable description: This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.
star: false
creator: Austin Che
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZa
sequence annotation: linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_E0051_sequence/1

lacZa
type: SequenceAnnotation
persistent identity: annotation2001726
display id: annotation2001726
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
type: Range
persistent identity: range2001726
display id: range2001726
version: 1
top level: BBa_E0051
direction: direction/forward
start: 1
end: 180
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2001727
display id: annotation2001727
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
type: Range
persistent identity: range2001727
display id: range2001727
version: 1
top level: BBa_E0051
direction: direction/forward
start: 181
end: 237
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2001728
display id: annotation2001728
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
type: Range
persistent identity: range2001728
display id: range2001728
version: 1
top level: BBa_E0051
direction: direction/forward
start: 238
end: 954
orientation: inline

https://synbiohub.org/public/igem/BBa_M36702_sequence/1
type: Sequence
persistent identity: BBa M36702 sequence
display id: BBa_M36702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgagtagtcttagttgtgctgaacgaaaagcgcacaacgatccttcgttcacagtggggaagttttcggatccatgacgtactagagtcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36709_sequence/1
type: Sequence
persistent identity: BBa M36709 sequence
display id: BBa_M36709_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36698_sequence/1
type: Sequence
persistent identity: BBa M36698 sequence
display id: BBa_M36698_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgagtagtcttagttgtgctgaacgaaaagcgcacaacgatccttcgttcacagtggggaagttttcggatccatgacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36001_sequence/1
type: Sequence
persistent identity: BBa M36001 sequence
display id: BBa_M36001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36010_sequence/1
type: Sequence
persistent identity: BBa M36010 sequence
display id: BBa_M36010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36706_sequence/1
type: Sequence
persistent identity: BBa M36706 sequence
display id: BBa_M36706_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcgcacccattcccgcgaaacgcggcagaaaacccgccgttgccaccgcaccagcgactggacaggttcagtctttaacgcgtggcctgaaattactggagtggattgccgaatccaatggcagtgtggcactcacggagctggcgcaacaagccgggttacccaattccacgacccaccgcctgctaaccacgatgcaacagcagggtttcgtgcgtcaggttggcgaactgggacactgggcaatcggcgcacatgcctttatggtcggcagcagctttctccagagccgtaatttgttagcaattgttcaccctatcctgcgcaatctaatggaagagtctggcgaaacggtcaatatggcggtgcttgatcaaagcgatcacgaagcgattattatcgaccaggtacagtgtacgcatctgatgcgaatgtccgcgcctatcggcggtaaattgccgatgcacgcttccggtgcgggtaaagcctttttagcccaactgagcgaagaacaggtgacgaagctgctgcaccgcaaagggttacatgcctatacccacgcaacgctggtgtctcctgtgcatttaaaagaagatctcgcccaaacgcgcaaacggggttattcatttgacgatgaggaacatgcgctggggctacgttgccttgcagcgtgtattttcgatgagcaccgtgaaccgtttgccgcaatttctatttccggaccgatttcacgtattaccgatgaccgcgtgaccgagtttggcgcgatggtgattaaagcggcgaaggaagtgacgctggcgtacggtggaatgcgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36699_sequence/1
type: Sequence
persistent identity: BBa M36699 sequence
display id: BBa_M36699_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacctcaggcaccttcgggtgccttttttatttccgaaacacacctcagtaggtgaataaattttattaatattgttatcaataagttatcaagtatttttaattaaaatggaaattgtttttgattttgatttttaaatgagtagtcttagttgtgctgaacgaaaagcgcacaacgatccttcgttcacagtggggaagttttcggatccatgacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0051_sequence/1
type: Sequence
persistent identity: BBa E0051 sequence
display id: BBa_E0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z