LOCUS       BBa_M36702              2262 bp    DNA     linear       11-Mar-2026
DEFINITION  Pyruvate Sensor v2 w/ Gemini
ACCESSION   BBa_M36702
VERSION     BBa_M36702.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             36..82
                     /label=BBa_M36001
     misc_feature    83..85
                     /label=Start Codon
     misc_feature    905..907
                     /label=Stop Codon
     CDS             83..907
                     /label=IclR Coding Region
     CDS             83..907
                     /label=BBa_M36706
     terminator      908..989
                     /label=BBa_M36010
     misc_feature    1000..1006
                     /label=IclR Box I
     misc_feature    1010..1016
                     /label=IclR Box II
     misc_feature    1019..1025
                     /label=IclR Box III
     misc_feature    1037..1043
                     /label=IclR Box IV
     misc_feature    1088..1094
                     /label=IclR Box V
     misc_feature    1065..1078
                     /label=IHF Binding Site
     misc_feature    1099..1105
                     /label=IclR Box VI
     misc_feature    1107..1113
                     /label=IclR Box VII
     misc_feature    1115..1120
                     /label=IclR Box VIII
     misc_feature    1103..1124
                     /label=CRP-cAMP Binding Site
     promoter        1108..1116
                     /label=-35 Region
     promoter        1132..1139
                     /label=-10 Region
     promoter        990..1207
                     /label=BBa_M36699
     misc_feature    1..1207
                     /label=BBa_M36698
     RBS             1216..1228
                     /label=RBS-3\Weak
     RBS             1222..1225
                     /label=RBS
     misc_feature    1216..1228
                     /label=BBa_B0032
     RBS             1216..1228
                     /label=BBa_B0032
     CDS             1229..1408
                     /label=lacZa
     misc_feature    1409..1465
                     /label=linker
     CDS             1466..2182
                     /label=GFP
     misc_feature    1229..2182
                     /label=BBa_E0051
     stem_loop       2194..2237
                     /label=stem_loop
     misc_feature    2183..2262
                     /label=BBa_B0010
     terminator      2183..2262
                     /label=BBa_B0010
     misc_feature    1216..2262
                     /label=BBa_M36709
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctatag agggtattaa taatgtatgg
       61 attaaagggg gaggcataac aaatggtcgc acccattccc gcgaaacgcg gcagaaaacc
      121 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
      181 actggagtgg attgccgaat ccaatggcag tgtggcactc acggagctgg cgcaacaagc
      241 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
      301 gcgtcaggtt ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
      361 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
      421 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
      481 tattatcgac caggtacagt gtacgcatct gatgcgaatg tccgcgccta tcggcggtaa
      541 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
      601 ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa cgctggtgtc
      661 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
      721 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgtga
      781 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg accgcgtgac
      841 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
      901 gcgctgatcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt
      961 ttggaggggc agaaagatga atgactgtct acctcaggca ccttcgggtg ccttttttat
     1021 ttccgaaaca cacctcagta ggtgaataaa ttttattaat attgttatca ataagttatc
     1081 aagtattttt aattaaaatg gaaattgttt ttgattttga tttttaaatg agtagtctta
     1141 gttgtgctga acgaaaagcg cacaacgatc cttcgttcac agtggggaag ttttcggatc
     1201 catgacgtac tagagtcaca caggaaagat gaccatgatt acggattcac tggccgtcgt
     1261 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1321 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1381 gttgcgcagc ctgaatggcg aatggcgcgc gggcggcagc gaaggcggcg gcagcgaaca
     1441 tcatcatcat catcatggca gcgaacgtaa aggagaagaa cttttcactg gagttgtccc
     1501 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     1561 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     1621 acctgttcca tggccaacac ttgtcactac cctgacctat ggtgttcaat gctttgcgag
     1681 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     1741 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     1801 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     1861 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     1921 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     1981 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     2041 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     2101 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     2161 ggatgaacta tacaaataat aaccaggcat caaataaaac gaaaggctca gtcgaaagac
     2221 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tc
//