BBa_M31517
type: ComponentDefinition
persistent identity: BBa M31517
display id: BBa_M31517
version: 1
was generated by: iGEM to SBOL conversion
description: Refactored M13KO7 genome from HpaI site in gene II through BamHI site in gene III
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:00Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1387
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: test
star: false
creator: Jennifer Logan
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence annotation: gene II
sequence annotation: promoter-g10
sequence annotation: rbs-g10
sequence annotation: gene X
sequence annotation: promoter g5
sequence annotation: rbs-g5
sequence annotation: g5 (ORF)
sequence annotation: BsrGI
sequence annotation: rbs-gVII
sequence annotation: rbs-g7
sequence annotation: gene VII
sequence annotation: promoter-g8
sequence annotation: rbs-g9
sequence annotation: gene IX
sequence annotation: SnaBI
sequence annotation: rbs-g8
sequence annotation: gene VIII
sequence annotation: ecoRI
sequence annotation: XmaI
sequence annotation: promoter-g3
sequence annotation: rbs-g3
sequence annotation: gene III (ORF)
sequence annotation: NIaIII
sequence annotation: myc epitope
sequence annotation: BamHI
sequence annotation: promoter-g6
sequence annotation: rbs-g6
sequence annotation: gene VI
sequence annotation: promoter-g1
sequence annotation: g1
sequence annotation: rbs-g11
sequence annotation: promoter-g4
sequence annotation: PacI
sequence annotation: rbs-g4
sequence annotation: rbs g-4
sequence annotation: g4
sequence annotation: ApaLI
sequence annotation: MscI
sequence annotation: NgoMIV
sequence annotation: DrdI
sequence annotation: SgrAI
sequence annotation: SacII
sequence annotation: BssSI
sequence annotation: BstBI
sequence annotation: AhdI
sequence annotation: SacI
sequence annotation: HindIII
sequence annotation: BsmBI
sequence annotation: PvuI
sequence annotation: EcoNI

gene II
type: SequenceAnnotation
persistent identity: annotation1914493
display id: annotation1914493
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914493/range1914493/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M31517/annotation1914493/range1914493/1
type: Range
persistent identity: range1914493
display id: range1914493
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1
end: 831
orientation: inline

promoter-g10
type: SequenceAnnotation
persistent identity: annotation1914441
display id: annotation1914441
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914441/range1914441/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M31517/annotation1914441/range1914441/1
type: Range
persistent identity: range1914441
display id: range1914441
version: 1
top level: BBa_M31517
direction: direction/forward
start: 381
end: 428
orientation: inline

rbs-g10
type: SequenceAnnotation
persistent identity: annotation1914442
display id: annotation1914442
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914442/range1914442/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914442/range1914442/1
type: Range
persistent identity: range1914442
display id: range1914442
version: 1
top level: BBa_M31517
direction: direction/forward
start: 480
end: 495
orientation: inline

gene X
type: SequenceAnnotation
persistent identity: annotation1914494
display id: annotation1914494
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914494/range1914494/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M31517/annotation1914494/range1914494/1
type: Range
persistent identity: range1914494
display id: range1914494
version: 1
top level: BBa_M31517
direction: direction/forward
start: 496
end: 831
orientation: inline

promoter g5
type: SequenceAnnotation
persistent identity: annotation1914440
display id: annotation1914440
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914440/range1914440/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M31517/annotation1914440/range1914440/1
type: Range
persistent identity: range1914440
display id: range1914440
version: 1
top level: BBa_M31517
direction: direction/forward
start: 786
end: 835
orientation: inline

rbs-g5
type: SequenceAnnotation
persistent identity: annotation1914443
display id: annotation1914443
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914443/range1914443/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M31517/annotation1914443/range1914443/1
type: Range
persistent identity: range1914443
display id: range1914443
version: 1
top level: BBa_M31517
direction: direction/forward
start: 827
end: 842
orientation: inline

g5 (ORF)
type: SequenceAnnotation
persistent identity: annotation1914444
display id: annotation1914444
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914444/range1914444/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M31517/annotation1914444/range1914444/1
type: Range
persistent identity: range1914444
display id: range1914444
version: 1
top level: BBa_M31517
direction: direction/forward
start: 843
end: 1106
orientation: inline

BsrGI
type: SequenceAnnotation
persistent identity: annotation1914445
display id: annotation1914445
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914445/range1914445/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914445/range1914445/1
type: Range
persistent identity: range1914445
display id: range1914445
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1021
end: 1025
orientation: inline

rbs-gVII
type: SequenceAnnotation
persistent identity: annotation1914952
display id: annotation1914952
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914952/range1914952/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M31517/annotation1914952/range1914952/1
type: Range
persistent identity: range1914952
display id: range1914952
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1092
end: 1107
orientation: inline

rbs-g7
type: SequenceAnnotation
persistent identity: annotation1914446
display id: annotation1914446
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914446/range1914446/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914446/range1914446/1
type: Range
persistent identity: range1914446
display id: range1914446
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1092
end: 1107
orientation: inline

gene VII
type: SequenceAnnotation
persistent identity: annotation1914495
display id: annotation1914495
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914495/range1914495/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M31517/annotation1914495/range1914495/1
type: Range
persistent identity: range1914495
display id: range1914495
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1108
end: 1209
orientation: inline

promoter-g8
type: SequenceAnnotation
persistent identity: annotation1914447
display id: annotation1914447
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914447/range1914447/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M31517/annotation1914447/range1914447/1
type: Range
persistent identity: range1914447
display id: range1914447
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1155
end: 1201
orientation: inline

rbs-g9
type: SequenceAnnotation
persistent identity: annotation1914448
display id: annotation1914448
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914448/range1914448/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914448/range1914448/1
type: Range
persistent identity: range1914448
display id: range1914448
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1190
end: 1205
orientation: inline

gene IX
type: SequenceAnnotation
persistent identity: annotation1914496
display id: annotation1914496
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914496/range1914496/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M31517/annotation1914496/range1914496/1
type: Range
persistent identity: range1914496
display id: range1914496
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1206
end: 1304
orientation: inline

SnaBI
type: SequenceAnnotation
persistent identity: annotation1914449
display id: annotation1914449
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914449/range1914449/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914449/range1914449/1
type: Range
persistent identity: range1914449
display id: range1914449
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1268
end: 1273
orientation: inline

rbs-g8
type: SequenceAnnotation
persistent identity: annotation1914450
display id: annotation1914450
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914450/range1914450/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_M31517/annotation1914450/range1914450/1
type: Range
persistent identity: range1914450
display id: range1914450
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1285
end: 1300
orientation: inline

gene VIII
type: SequenceAnnotation
persistent identity: annotation1914451
display id: annotation1914451
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914451/range1914451/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M31517/annotation1914451/range1914451/1
type: Range
persistent identity: range1914451
display id: range1914451
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1301
end: 1522
orientation: inline

ecoRI
type: SequenceAnnotation
persistent identity: annotation1915198
display id: annotation1915198
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1915198/range1915198/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1915198/range1915198/1
type: Range
persistent identity: range1915198
display id: range1915198
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1523
end: 1528
orientation: inline

XmaI
type: SequenceAnnotation
persistent identity: annotation1915199
display id: annotation1915199
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1915199/range1915199/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1915199/range1915199/1
type: Range
persistent identity: range1915199
display id: range1915199
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1529
end: 1534
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1914452
display id: annotation1914452
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914452/range1914452/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M31517/annotation1914452/range1914452/1
type: Range
persistent identity: range1914452
display id: range1914452
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1535
end: 1582
orientation: inline

rbs-g3
type: SequenceAnnotation
persistent identity: annotation1914453
display id: annotation1914453
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914453/range1914453/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914453/range1914453/1
type: Range
persistent identity: range1914453
display id: range1914453
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1598
end: 1613
orientation: inline

gene III (ORF)
type: SequenceAnnotation
persistent identity: annotation1914454
display id: annotation1914454
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914454/range1914454/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_M31517/annotation1914454/range1914454/1
type: Range
persistent identity: range1914454
display id: range1914454
version: 1
top level: BBa_M31517
direction: direction/forward
start: 1614
end: 2289
orientation: inline

NIaIII
type: SequenceAnnotation
persistent identity: annotation1915212
display id: annotation1915212
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1915212/range1915212/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1915212/range1915212/1
type: Range
persistent identity: range1915212
display id: range1915212
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2192
end: 2195
orientation: inline

myc epitope
type: SequenceAnnotation
persistent identity: annotation1915214
display id: annotation1915214
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1915214/range1915214/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1915214/range1915214/1
type: Range
persistent identity: range1915214
display id: range1915214
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2196
end: 2229
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1914455
display id: annotation1914455
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914455/range1914455/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914455/range1914455/1
type: Range
persistent identity: range1914455
display id: range1914455
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2289
end: 2289
orientation: inline

promoter-g6
type: SequenceAnnotation
persistent identity: annotation1914457
display id: annotation1914457
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914457/range1914457/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M31517/annotation1914457/range1914457/1
type: Range
persistent identity: range1914457
display id: range1914457
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2716
end: 2764
orientation: inline

promoter-g6
type: SequenceAnnotation
persistent identity: annotation1914456
display id: annotation1914456
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914456/range1914456/1
role: feature/promoter
role: SO:0000167

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type: Range
persistent identity: range1914456
display id: range1914456
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2716
end: 2764
orientation: inline

rbs-g6
type: SequenceAnnotation
persistent identity: annotation1914458
display id: annotation1914458
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914458/range1914458/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914458/range1914458/1
type: Range
persistent identity: range1914458
display id: range1914458
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2840
end: 2855
orientation: inline

gene VI
type: SequenceAnnotation
persistent identity: annotation1914459
display id: annotation1914459
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914459/range1914459/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range1914459
display id: range1914459
version: 1
top level: BBa_M31517
direction: direction/forward
start: 2856
end: 3194
orientation: inline

promoter-g1
type: SequenceAnnotation
persistent identity: annotation1914460
display id: annotation1914460
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914460/range1914460/1
role: SO:0000167
role: feature/promoter

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type: Range
persistent identity: range1914460
display id: range1914460
version: 1
top level: BBa_M31517
direction: direction/forward
start: 3086
end: 3132
orientation: inline

g1
type: SequenceAnnotation
persistent identity: annotation1914461
display id: annotation1914461
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914461/range1914461/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range1914461
display id: range1914461
version: 1
top level: BBa_M31517
direction: direction/forward
start: 3196
end: 4242
orientation: inline

rbs-g11
type: SequenceAnnotation
persistent identity: annotation1914462
display id: annotation1914462
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914462/range1914462/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range1914462
display id: range1914462
version: 1
top level: BBa_M31517
direction: direction/forward
start: 3900
end: 3915
orientation: inline

promoter-g4
type: SequenceAnnotation
persistent identity: annotation1914463
display id: annotation1914463
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914463/range1914463/1
role: feature/promoter
role: SO:0000167

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type: Range
persistent identity: range1914463
display id: range1914463
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4055
end: 4103
orientation: inline

PacI
type: SequenceAnnotation
persistent identity: annotation1914464
display id: annotation1914464
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914464/range1914464/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range1914464
display id: range1914464
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4131
end: 4138
orientation: inline

rbs-g4
type: SequenceAnnotation
persistent identity: annotation1914465
display id: annotation1914465
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914465/range1914465/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range1914465
display id: range1914465
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4204
end: 4219
orientation: inline

rbs g-4
type: SequenceAnnotation
persistent identity: annotation1914466
display id: annotation1914466
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914466/range1914466/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range1914466
display id: range1914466
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4204
end: 4219
orientation: inline

g4
type: SequenceAnnotation
persistent identity: annotation1914467
display id: annotation1914467
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914467/range1914467/1
role: feature/protein
role: SO:0000316

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type: Range
persistent identity: range1914467
display id: range1914467
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4220
end: 5500
orientation: inline

ApaLI
type: SequenceAnnotation
persistent identity: annotation1914468
display id: annotation1914468
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914468/range1914468/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range1914468
display id: range1914468
version: 1
top level: BBa_M31517
direction: direction/forward
start: 4743
end: 4748
orientation: inline

MscI
type: SequenceAnnotation
persistent identity: annotation1914469
display id: annotation1914469
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914469/range1914469/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range1914469
display id: range1914469
version: 1
top level: BBa_M31517
direction: direction/forward
start: 5080
end: 5085
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation1914470
display id: annotation1914470
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914470/range1914470/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914470/range1914470/1
type: Range
persistent identity: range1914470
display id: range1914470
version: 1
top level: BBa_M31517
direction: direction/forward
start: 5613
end: 5617
orientation: inline

DrdI
type: SequenceAnnotation
persistent identity: annotation1914471
display id: annotation1914471
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914471/range1914471/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914471/range1914471/1
type: Range
persistent identity: range1914471
display id: range1914471
version: 1
top level: BBa_M31517
direction: direction/forward
start: 5763
end: 5765
orientation: inline

SgrAI
type: SequenceAnnotation
persistent identity: annotation1914472
display id: annotation1914472
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914472/range1914472/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914472/range1914472/1
type: Range
persistent identity: range1914472
display id: range1914472
version: 1
top level: BBa_M31517
direction: direction/forward
start: 5867
end: 5871
orientation: inline

SacII
type: SequenceAnnotation
persistent identity: annotation1914473
display id: annotation1914473
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914473/range1914473/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914473/range1914473/1
type: Range
persistent identity: range1914473
display id: range1914473
version: 1
top level: BBa_M31517
direction: direction/forward
start: 6030
end: 6035
orientation: inline

BssSI
type: SequenceAnnotation
persistent identity: annotation1914474
display id: annotation1914474
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914474/range1914474/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914474/range1914474/1
type: Range
persistent identity: range1914474
display id: range1914474
version: 1
top level: BBa_M31517
direction: direction/forward
start: 6158
end: 6162
orientation: inline

BstBI
type: SequenceAnnotation
persistent identity: annotation1914475
display id: annotation1914475
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914475/range1914475/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914475/range1914475/1
type: Range
persistent identity: range1914475
display id: range1914475
version: 1
top level: BBa_M31517
direction: direction/forward
start: 6518
end: 6520
orientation: inline

AhdI
type: SequenceAnnotation
persistent identity: annotation1914476
display id: annotation1914476
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914476/range1914476/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914476/range1914476/1
type: Range
persistent identity: range1914476
display id: range1914476
version: 1
top level: BBa_M31517
direction: direction/forward
start: 6824
end: 6834
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1914477
display id: annotation1914477
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914477/range1914477/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914477/range1914477/1
type: Range
persistent identity: range1914477
display id: range1914477
version: 1
top level: BBa_M31517
direction: direction/forward
start: 6951
end: 6956
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1914478
display id: annotation1914478
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914478/range1914478/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914478/range1914478/1
type: Range
persistent identity: range1914478
display id: range1914478
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7402
end: 7406
orientation: inline

HindIII
type: SequenceAnnotation
persistent identity: annotation1914479
display id: annotation1914479
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914479/range1914479/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914479/range1914479/1
type: Range
persistent identity: range1914479
display id: range1914479
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7402
end: 7407
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation1914480
display id: annotation1914480
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914480/range1914480/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914480/range1914480/1
type: Range
persistent identity: range1914480
display id: range1914480
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7504
end: 7508
orientation: inline

PvuI
type: SequenceAnnotation
persistent identity: annotation1914481
display id: annotation1914481
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914481/range1914481/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914481/range1914481/1
type: Range
persistent identity: range1914481
display id: range1914481
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7524
end: 7526
orientation: inline

EcoNI
type: SequenceAnnotation
persistent identity: annotation1914482
display id: annotation1914482
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914482/range1914482/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914482/range1914482/1
type: Range
persistent identity: range1914482
display id: range1914482
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7611
end: 7612
orientation: inline

NruI
type: SequenceAnnotation
persistent identity: annotation1914483
display id: annotation1914483
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914483/range1914483/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914483/range1914483/1
type: Range
persistent identity: range1914483
display id: range1914483
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7865
end: 7870
orientation: inline

XhoI
type: SequenceAnnotation
persistent identity: annotation1914484
display id: annotation1914484
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914484/range1914484/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914484/range1914484/1
type: Range
persistent identity: range1914484
display id: range1914484
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7922
end: 7926
orientation: inline

TliI
type: SequenceAnnotation
persistent identity: annotation1914485
display id: annotation1914485
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914485/range1914485/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M31517/annotation1914485/range1914485/1
type: Range
persistent identity: range1914485
display id: range1914485
version: 1
top level: BBa_M31517
direction: direction/forward
start: 7922
end: 7926
orientation: inline

Pst1
type: SequenceAnnotation
persistent identity: annotation1914486
display id: annotation1914486
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914486/range1914486/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914486/range1914486/1
type: Range
persistent identity: range1914486
display id: range1914486
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8079
end: 8083
orientation: inline

promoter-g2
type: SequenceAnnotation
persistent identity: annotation1914487
display id: annotation1914487
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914487/range1914487/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_M31517/annotation1914487/range1914487/1
type: Range
persistent identity: range1914487
display id: range1914487
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8188
end: 8235
orientation: inline

SwaI
type: SequenceAnnotation
persistent identity: annotation1914488
display id: annotation1914488
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914488/range1914488/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914488/range1914488/1
type: Range
persistent identity: range1914488
display id: range1914488
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8202
end: 8209
orientation: inline

rbs-g1
type: SequenceAnnotation
persistent identity: annotation1914489
display id: annotation1914489
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914489/range1914489/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914489/range1914489/1
type: Range
persistent identity: range1914489
display id: range1914489
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8252
end: 8267
orientation: inline

rbs-g2
type: SequenceAnnotation
persistent identity: annotation1914490
display id: annotation1914490
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914490/range1914490/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_M31517/annotation1914490/range1914490/1
type: Range
persistent identity: range1914490
display id: range1914490
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8252
end: 8267
orientation: inline

AccI
type: SequenceAnnotation
persistent identity: annotation1914491
display id: annotation1914491
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914491/range1914491/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914491/range1914491/1
type: Range
persistent identity: range1914491
display id: range1914491
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8353
end: 8358
orientation: inline

HpaI
type: SequenceAnnotation
persistent identity: annotation1914492
display id: annotation1914492
version: 1
top level: BBa_M31517
location: https://synbiohub.org/public/igem/BBa_M31517/annotation1914492/range1914492/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M31517/annotation1914492/range1914492/1
type: Range
persistent identity: range1914492
display id: range1914492
version: 1
top level: BBa_M31517
direction: direction/forward
start: 8669
end: 8669
orientation: inline

https://synbiohub.org/public/igem/BBa_M31517_sequence/1
type: Sequence
persistent identity: BBa M31517 sequence
display id: BBa_M31517_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaatttacctcgaaagcaagttgagaattccccgggaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaattcctttagttgttcctttctattctcactccgctgaaactgttgaaagttgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaagacgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatgctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtacatgggttcctattgggcttgctatccctgaaaatgagggtggtggctctgagggtggcggttctgagggtggcggttctgagggtggcggtactaaacctcctgagtacggtgatacacctattccgggctatacttatatcaaccctctcgacggcacttatccg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z