LOCUS       BBa_M10215              2921 bp    DNA     linear       09-Mar-2026
DEFINITION  {Pbad.rbs.prepro.StrepTag}{<AG4>}{<ehaB!]{dblTerm}
ACCESSION   BBa_M10215
VERSION     BBa_M10215.1  
FEATURES             Location/Qualifiers
     CDS             50..805
                     /label=AraC
     promoter        956..1193
                     /label=Pbad
     CDS             1290..1358
                     /label=PrePro Sequence
     CDS             1374..1412
                     /label=Streptavidin Binding Peptide
     RBS             1274..1289
                     /label=rbs
     misc_feature    1..1452
                     /label=BBa_M10093
     misc_feature    1461..1466
                     /label=BBa_K112999
     CDS             1475..1510
                     /label=BBa_M10023
     misc_feature    1519..1524
                     /label=BBa_K112999
     CDS             1533..2764
                     /label=BBa_M10052
     misc_feature    2773..2778
                     /label=BBa_K112999
     terminator      2787..2921
                     /label=BBa_K112903
ORIGIN
        1 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
       61 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      121 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      181 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      241 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
      301 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      361 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      421 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
      481 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      541 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
      601 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
      661 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
      721 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
      781 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
      841 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
      901 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
      961 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1021 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1081 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1141 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcttacctga
     1201 cgctttttat cgcaactctc tactgtttct ccatacccgg atctttgggc tagcgcattc
     1261 gagctcggta cctttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
     1321 gttctggctt tcaccgcagg tacttccgta gctggccagt ctggccagtg ggtgtgccac
     1381 ccgatgtggg aggtgatgtg cctgagggga gggtctgggt ggaggggcat ggagggaggg
     1441 cagtctgggc agtactagag ggatcttact agagaacccg tcttctctgt tccgttacct
     1501 gccgtctgac tactagaggg atcttactag aggatctggc ttaatcgcaa tgaactctga
     1561 tggtcacctg actattaacg gtaacggcga taacgacaac actgcttcta tcgaagctgg
     1621 tcagaacgaa gttgataaca acggtgacca tgttgcagcc gcgaccggta actacaaagt
     1681 tcgtatcgac aacgctactg gtgctggttc tatcgctgac tacaacggca acgagctgat
     1741 ctacgtcaac gacaaaaaca gcaacgcgac cttctctgct gctaacaaag ctgacctggg
     1801 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagtt
     1861 gaccgactac gctaacatgg cgctgagcat cccatctgcg aacaccaata tctggaacct
     1921 ggaacaagac accgttggta ctcgtttgac caactctcgt catggcctgg ctgataacgg
     1981 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
     2041 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
     2101 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg accgttctgg
     2161 tcaggtggat caagacagcc agactgccta catctactct tctgctcact tcgcgaacaa
     2221 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     2281 gagcaacggt acttacgttg acggtagcac caactccgac gcttggggct tcggtttgaa
     2341 agccggttac gacttcaaac tgggtgatgc tggttacgtg actccttacg cagcatttct
     2401 ggtctgttcc agtctggtga tgactaccag ctgagcaacg acatgaaagt tgacggtcag
     2461 tcttacgaca gcatgcgtta tgaactgggt gtagatgcag gttatacctt cacctacagc
     2521 gaagatcagg ctctgactcc gtacttcaaa ctggcttacg tctacgacga ctctaacaac
     2581 gataacgatg tgaacggtga ttccatcgat aacggtactg aagggtctgc ggtacgtgtt
     2641 ggtctgggta ctcagttcag cttcaccaag aacttcagcg cctataccga tgctaactac
     2701 ctcggtggtg gtgacgtaga tcaagactgg tccgcgaacg tgggtgttaa atatacctgg
     2761 taagtactag agggatctta ctagaggatc tccaggcatc aaataaaacg aaaggctcag
     2821 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2881 cacactggct caccttcggg tgggcctttc tgcgtttata g
//