##gff-version 3
##sequence-region BBa_K750109 1 2969
BBa_K750109	.	terminator	1781	1909	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K750109	.	terminator	1781	1860	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750109	.	engineered_region	1781	1860	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750109	.	stem_loop	1792	1835	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750109	.	terminator	1869	1909	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750109	.	stop_codon	1902	1902	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750109	.	polyA_site	1896	1909	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750109	.	stem_loop	1876	1895	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750109	.	engineered_region	1869	1909	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750109	.	engineered_region	939	2969	.	+	0	ID=BBa_K750108;Name=BBa_K750108
BBa_K750109	.	engineered_region	939	1909	.	+	0	ID=BBa_K750103;Name=BBa_K750103;Parent=BBa_K750108
BBa_K750109	.	ribosome_entry_site	996	1007	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750103
BBa_K750109	.	conserved_region	1000	1003	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750109	.	promoter	939	987	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K750103
BBa_K750109	.	engineered_region	939	987	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K750109	.	promoter	976	981	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K750109	.	non_covalent_binding_site	963	979	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K750109	.	promoter	953	958	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K750109	.	non_covalent_binding_site	939	955	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K750109	.	engineered_region	1014	1772	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750103
BBa_K750109	.	stop_codon	1767	1769	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750109	.	CDS	1017	1729	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750109	.	start_codon	1014	1016	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750109	.	tag	1730	1766	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750109	.	stop_codon	1770	1772	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750109	.	terminator	1781	1909	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750103
BBa_K750109	.	terminator	1781	1860	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750109	.	engineered_region	1781	1860	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750109	.	stem_loop	1792	1835	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750109	.	terminator	1869	1909	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750109	.	stop_codon	1902	1902	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750109	.	polyA_site	1896	1909	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750109	.	stem_loop	1876	1895	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750109	.	engineered_region	1869	1909	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750109	.	engineered_region	1918	2969	.	+	0	ID=BBa_K750000;Name=BBa_K750000;Parent=BBa_K750108
BBa_K750109	.	terminator	2841	2969	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750000
BBa_K750109	.	terminator	2841	2920	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750109	.	engineered_region	2841	2920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750109	.	stem_loop	2852	2895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750109	.	terminator	2929	2969	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750109	.	stop_codon	2962	2962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750109	.	polyA_site	2956	2969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750109	.	stem_loop	2936	2955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750109	.	engineered_region	2929	2969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750109	.	ribosome_entry_site	2056	2067	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750000
BBa_K750109	.	conserved_region	2060	2063	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750109	.	engineered_region	2074	2832	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K750000
BBa_K750109	.	stop_codon	2827	2829	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K750109	.	CDS	2077	2789	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K750109	.	start_codon	2074	2076	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K750109	.	tag	2790	2826	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K750109	.	stop_codon	2830	2832	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K750109	.	promoter	1918	2047	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750000
BBa_K750109	.	promoter	1918	2048	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750109	.	operator	1978	1995	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750109	.	operator	1957	1974	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750109	.	CDS	19	793	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K750109	.	engineered_tag	769	793	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K750109	.	CDS	22	729	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K750109	.	tag	730	762	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K750109	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K750109	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750109	.	terminator	802	930	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K750109	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750109	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750109	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750109	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750109	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750109	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750109	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750109	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750109	.	terminator	2841	2969	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K750109	.	terminator	2841	2920	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750109	.	engineered_region	2841	2920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750109	.	stem_loop	2852	2895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750109	.	terminator	2929	2969	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750109	.	stop_codon	2962	2962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750109	.	polyA_site	2956	2969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750109	.	stem_loop	2936	2955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750109	.	engineered_region	2929	2969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K750109
aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagat
cgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtg
cgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgc
aaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataag
attagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactaga
gaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata