##gff-version 3
##sequence-region BBa_K743010 1 5032
BBa_K743010	.	sequence_feature	4589	5032	.	+	0	ID=annotation2193820;Name=3' recombination site
BBa_K743010	.	promoter	1	219	.	+	0	ID=annotation2193803;Name=Spectinomycin resistance promoter
BBa_K743010	.	primer_binding_site	1012	1031	.	+	0	ID=annotation2193805;Name=Verification forward primer binding site
BBa_K743010	.	stop_codon	1111	1121	.	+	0	ID=annotation2193807;Name=three stop codons
BBa_K743010	.	primer_binding_site	2341	2360	.	+	0	ID=annotation2193818;Name=Verification revers primer binding site
BBa_K743010	.	stem_loop	2272	2340	.	+	0	ID=annotation2193817;Name=B0054 terminator
BBa_K743010	.	conserved_region	1135	1156	.	+	0	ID=annotation2193814;Name=Biobrick Prefix
BBa_K743010	.	conserved_region	2226	2246	.	+	0	ID=annotation2193813;Name=biobrick Suffix
BBa_K743010	.	stem_loop	2097	2225	.	+	0	ID=annotation2193812;Name=B0015 double terminator
BBa_K743010	.	CDS	220	1011	.	+	0	ID=annotation2193804;Name=Spectinomycin resistance
BBa_K743010	.	conserved_region	2247	2259	.	+	0	ID=annotation2193815;Name=TOPO A cloning site
BBa_K743010	.	promoter	1157	1356	.	+	0	ID=annotation2193809;Name=lacI promoter
BBa_K743010	.	conserved_region	1112	1156	.	+	0	ID=annotation2193808;Name=TOPO A cloning site BBa_B0043
BBa_K743010	.	CDS	1383	2057	.	+	0	ID=annotation2193810;Name=BBa_E1010 mRFP
BBa_K743010	.	sequence_feature	2366	2832	.	+	0	ID=annotation2193819;Name=5?? recombination site
BBa_K743010	.	stem_loop	1032	1110	.	+	0	ID=annotation2193806;Name=B0055 terminator
BBa_K743010	.	stop_codon	2260	2271	.	+	0	ID=annotation2193816;Name=three stop codons
BBa_K743010	.	conserved_region	2833	4588	.	+	0	ID=annotation2193821;Name=plasmid backbone
BBa_K743010	.	stop_codon	2058	2063	.	+	0	ID=annotation2193811;Name=stop codons
>BBa_K743010
atgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttgtt
atgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgat
gttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgagggaagcggtgatc
gccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatt
tgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttga
tgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgct
gtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaat
ggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagc
aagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgag
gcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgt
cccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgcc
ggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgca
gatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataatgccacctgacgtcta
agaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacg
taattagttagttagcccttagtgactcgaattcgcggccgcttctagagcaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga
gttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggata
acaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtt
tcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaagg
tacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccag
tacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat
gggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcat
ctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagct
tccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtc
actacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaa
actggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagc
ctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagatgctga
tattgcccacgaattacgtacaccgattacggcgattcaggcaacgttggaaacgacattaaatgcagaacctaatgct
gaagaaacccatagcaccttgcaaaccttaaaacgacaaaattatcgtctcagccatttaatccatgatttattactcc
tctcccgcatggatttaacaacagttaatccgacccagtttaccctttgttgtctcaatgatttagttgaagatttaac
cgaagaatttgccagtttggcgatcgccgctggggtattgttatctgcgaagttagacaatcaagcaaatatctgggtc
aggggagaagaagaacagctttatcgtttggtgggtaatttaatcagtaatgccattcactacaccccaacggggggcg
aggtaaccgtcatgcttgagaccgataaacagcaagccatcatcaaagtccaagatacgggcattggggttatccacag
aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgct
ggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgaca
ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggat
acctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt
cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaata
aaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacagga
gtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgc
cgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataata
tttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccag
ggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacat
cttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctc
atggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccgga
tgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaa
aggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacg
atgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctc
gataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaact
cgagtgcaaattttgcctaatctttgcttaggtacagtagcttgccctaggtcgcctcaacggaggcatattctcaacg
caacccaacagccgggctattatgtccggtttttggacttaaacggtcaattattagcaacggcaggggaaaatccccc
aggcttagcctttgagcgggaaaccacccgtcaaaaaccattaacggatcaaaaagggaaccgctatcaccaagtttct
ttgctgttaaaaaccaccactggtgaaccttggggctatttaaaggtggggcgctccttggtggaatatgaccatcatt
tgcacacgatccaaggctttttagtttggggtttacccattgtgatgattgtggtcggtggtgctagttggtggttagc
aggcttagccatggagccggtttatcgttcctaccaacaaatccagcagttcacc