##gff-version 3
##sequence-region BBa_K734006 2 6989
BBa_K734006	.	promoter	1563	1631	.	+	0	ID=annotation2205451;Name=lacUV5 
BBa_K734006	.	sequence_feature	2437	2565	.	+	0	ID=annotation2205455;Name=terminator
BBa_K734006	.	operator	1569	1589	.	+	0	ID=annotation2205443;Name=lacO
BBa_K734006	.	promoter	2689	2717	.	+	0	ID=annotation2205456;Name=ampR promoter
BBa_K734006	.	ribosome_entry_site	1666	1676	.	+	0	ID=annotation2205453;Name=strong RBS
BBa_K734006	.	CDS	837	1517	.	+	0	ID=annotation2205448;Name=rfp
BBa_K734006	.	ribosome_entry_site	1518	1528	.	+	0	ID=annotation2205449;Name=strong RBS
BBa_K734006	.	sequence_feature	1529	1562	.	+	0	ID=annotation2205450;Name=mutant loxP
BBa_K734006	.	CDS	190	795	.	+	0	ID=annotation2205446;Name=lasI
BBa_K734006	.	CDS	1677	2406	.	+	0	ID=annotation2205454;Name=sfGFP
BBa_K734006	.	sequence_feature	1632	1665	.	+	0	ID=annotation2205452;Name=mutant loxP
BBa_K734006	.	sequence_feature	2	159	.	+	0	ID=annotation2205445;Name=rrnB terminator
BBa_K734006	.	CDS	5808	6989	.	+	0	ID=annotation2205458;Name=lacI
BBa_K734006	.	CDS	2759	3619	.	+	0	ID=annotation2205444;Name=ampR
BBa_K734006	.	ribosome_entry_site	796	806	.	+	0	ID=annotation2205447;Name=strong RBS
BBa_K734006	.	sequence_feature	3774	4393	.	+	0	ID=annotation2205457;Name=rep_origin
>BBa_K734006
aaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagaccccac
actaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggc
agggtcgcatctgtttctcttgagcttcttgttaggaaacagccagacgctgttcaaccagaacaccaccgtacagagc
gatctgggttttagcgttcagttcgatacgcagagcaacagcacgttcgataccgattttcaggtgcggaccgaaacgg
gaaacgtccagaccagcacggatcatcattttttcaacaccaacggtggtaacggtaaccagggtctggatgtcgttct
gcaaggagtaacgagccagagcacgcatagcttccagggtgcagtcggagaaacccagggaacctttctgaccggagtt
gatagcgaaacgggacagttcccagatgtgcggggagcacggagcttctttaccgtgcagcagttccgggaaggtgttt
ttcagcatgtacggaccggtggtgtcgaagatacgccagcaaccgaaaacctgagcttccggggtgtcttcctggatca
gcatgtagtacggggacagagcgtcgtaaccgtcgatttccatttcgtcgataacggaaacgtcccaacctttacgttc
tttgaaaacctgagcacgcagtttgtgcatttcacccagcagttttttgtcgaactcttcacgacgaccgatctgaacg
atcatatgtgtttcctagatgcttgggatttcggtgggttggttgtttattaagcaccggtggagtgacgaccttcagc
acgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcaccc
ggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagac
gcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggttttttt
ctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggag
gagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggaca
gtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtc
ccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcacct
tcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgt
cttcggaggaagccatatgtgtttcctataacttcgtatagcatacattatacgaagttatgtgtgaaattgttatccg
ctcacaattccacacattatacgagccggaagcataaagtgtaaagcctggtaccgttcgtataatgtatgctatacga
acggtaaggaaacacatatggcatccaagggcgaggagctctttactggcgtagtaccaattctcgtagagctcgatgg
cgatgtaaatggccataagttttccgtacgcggcgagggcgagggcgatgcaactaacggcaagctcactctcaagttt
atttgtactactggcaagctcccagtaccatggccaactctcgtaactactctgacctatggcgtacaatgtttttccc
gctatccagatcacatgaagcaacatgatttttttaagtccgcaatgccagagggctatgtacaagagcgcactattag
ctttaaggatgatggcacctataagactcgcgcagaggtaaagtttgagggcgatactctcgtaaatcgcattgagctc
aagggcattgattttaaggaggatggcaatattctcggccataagctggagtataatttcaattcccataatgtatata
ttaccgcagataagcaaaagaatggcattaaggcgaattttaagattcgccataatgtggaggatggctccgtacaact
cgcagatcattatcaacaaaatactccaattggcgatggcccagtactcctcccagataatcattatctctccactcaa
tccgtgctctccaaagatccaaatgagaagcgcgatcacatggtactcctggagtttgtaactgcagcaggcattactc
atggcatggatgagctctataagctcgagatgctaaagggatgttatgcgtgttgtgagggtttggccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttataaaatgagctgatttaacaaaaatttaacgcgaattttaacaa
aatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaata
cattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagta
ttcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggt
gaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatcctt
gagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgta
ttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacaga
aaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaac
ttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttg
atcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaac
gttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaa
gttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggt
ctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggc
aactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtt
tactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatc
tcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg
agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggat
caagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagc
cgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgc
tgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctga
acggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgag
aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgag
ggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttg
tgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggc
cttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgatac
cgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctc
cttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaag
ccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct
gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttc
accgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcc
tgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaaggg
cggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacg
agagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcg
gtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccac
agggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagacttta
cgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgc
tcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacga
tcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgac
gaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcgg
tcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcgg
cgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccgg
tgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccag
ctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagt
gagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcccca
gcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactac
cgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaacc
agcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcctt
cccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacaga
acttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtct
tcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcag
cttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtg
caccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccccaccgctgagcaataactagcataac
cccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggatacgctggcacccagtt
gatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcag
caacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttt
tcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctg
cgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcg
aaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag
tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggcc
acggggcctgccaccatacccacgccgaaacaagcgctca