##gff-version 3
##sequence-region BBa_K731520 1 2123
BBa_K731520	.	promoter	1	1239	.	+	0	ID=BBa_K731500;Name=BBa_K731500
BBa_K731520	.	ribosome_entry_site	1085	1092	.	+	0	ID=annotation2181542;Name=rbs;Parent=BBa_K731500
BBa_K731520	.	start_codon	1081	1084	.	+	0	ID=annotation2181541;Name=start;Parent=BBa_K731500
BBa_K731520	.	promoter	1162	1188	.	+	0	ID=annotation2181546;Name=Ptac promoter;Parent=BBa_K731500
BBa_K731520	.	stop_codon	1	3	.	+	0	ID=annotation2181539;Name=stop;Parent=BBa_K731500
BBa_K731520	.	CDS	1	1084	.	+	0	ID=annotation2181540;Name=LacI;Parent=BBa_K731500
BBa_K731520	.	operator	1194	1219	.	+	0	ID=annotation2181545;Name=lacO operator;Parent=BBa_K731500
BBa_K731520	.	engineered_region	1	1153	.	+	0	ID=annotation2181544;Name=BBa_K731300;Parent=BBa_K731500
BBa_K731520	.	promoter	1093	1153	.	+	0	ID=annotation2181543;Name=LacIq promoter;Parent=BBa_K731500
BBa_K731520	.	engineered_region	1248	2123	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K731520	.	terminator	2083	2123	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K731520	.	stop_codon	2116	2116	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K731520	.	polyA_site	2110	2123	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K731520	.	stem_loop	2090	2109	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K731520	.	engineered_region	2083	2123	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K731520	.	terminator	1995	2074	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K731520	.	engineered_region	1995	2074	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K731520	.	stem_loop	2006	2049	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K731520	.	ribosome_entry_site	1248	1260	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K731520	.	ribosome_entry_site	1248	1260	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K731520	.	engineered_region	1248	1260	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K731520	.	ribosome_entry_site	1254	1257	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K731520	.	CDS	1267	1986	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K731520	.	CDS	1267	1986	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K731520
tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtt
tgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccct
gagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggat
ataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatg
gcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgca
tggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagata
tttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc
aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcag
agacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagtt
aatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctacc
atcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcaggg
ccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaatt
cagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacg
gtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactct
cttccgggcgctatcatgccataccgcgaaaggttttgcgccattctactagaggttgacaattaatcatcggctcgta
taatgtgtgggaattgtgagcggataacaattccctcatgaaataattttgttttactagagtcacacaggaaagtact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata