LOCUS BBa_K551001 10878 bp DNA linear 12-Mar-2026 DEFINITION plasmid of insertion and deletion ACCESSION BBa_K551001 VERSION BBa_K551001.1 FEATURES Location/Qualifiers CDS 9818..10678 /label=AmpR CDS 707..1510 /label=URA3 CDS 2504..3392 /label=AraC CDS 3737..4152 /label=gam CDS 4159..4944 /label=bet CDS 4941..5621 /label=exo CDS 5805..7076 /label=Flipase CDS 7076..7890 /label=CI857ts CDS 7936..8889 /label=RepA101ts misc_feature 8894..9634 /label=OriR101 promoter 3658..3685 /label=pARAb ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct 481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 601 gctttccagt cgggaaacct gtcgtgccag cagatctgag ctttttcttt ccaatttttt 661 ttttttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc 721 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag 781 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga 841 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca 901 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag 961 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat 1021 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag 1081 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca 1141 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact 1201 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt 1261 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga 1321 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta 1381 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga 1441 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag 1501 ctttcgacat gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac 1561 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg 1621 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa 1681 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgctgcat 1741 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 1801 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agcatcgatg 1861 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga 1921 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt 1981 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact 2041 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt 2101 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag 2161 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt 2221 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc 2281 ctatactttc tagctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa 2341 acgagcgctt ccgaaaatgc aacgcgagct gccatcgatg ctcactcaaa ggtcggtaat 2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2461 aaaggccagg aaccgtaaaa aggctgccac ctgcatcgat ttattatgac aacttgacgg 2521 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2581 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2641 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2701 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2761 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2821 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2881 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2941 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 3001 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 3061 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 3121 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 3181 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 3241 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 3301 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3361 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3421 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3481 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3541 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3601 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3661 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggaa ttcgagctct 3721 aaggaggtta taaaaaatgg atattaatac tgaaactgag atcaagcaaa agcattcact 3781 aacccccttt cctgttttcc taatcagccc ggcatttcgc gggcgatatt ttcacagcta 3841 tttcaggagt tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg 3901 ggcgcgtcac taccagcagc tcgcccgtga agagaaagag gcagaactgg cagacgacat 3961 ggaaaaaggc ctgccccagc acctgtttga atcgctatgc atcgatcatt tgcaacgcca 4021 cggggccagc aaaaaatcca ttacccgtgc gtttgatgac gatgttgagt ttcaggagcg 4081 catggcagaa cacatccggt acatggttga aaccattgct caccaccagg ttgatattga 4141 ttcagaggta taaaacgaat gagtactgca ctcgcaacgc tggctgggaa gctggctgaa 4201 cgtgtcggca tggattctgt cgacccacag gaactgatca ccactcttcg ccagacggca 4261 tttaaaggtg atgccagcga tgcgcagttc atcgcattac tgatcgttgc caaccagtac 4321 ggccttaatc cgtggacgaa agaaatttac gcctttcctg ataagcagaa tggcatcgtt 4381 ccggtggtgg gcgttgatgg ctggtcccgc atcatcaatg aaaaccagca gtttgatggc 4441 atggactttg agcaggacaa tgaatcctgt acatgccgga tttaccgcaa ggaccgtaat 4501 catccgatct gcgttaccga atggatggat gaatgccgcc gcgaaccatt caaaactcgc 4561 gaaggcagag aaatcacggg gccgtggcag tcgcatccca aacggatgtt acgtcataaa 4621 gccatgattc agtgtgcccg tctggccttc ggatttgctg gtatctatga caaggatgaa 4681 gccgagcgca ttgtcgaaaa tactgcatac actgcagaac gtcagccgga acgcgacatc 4741 actccggtta acgatgaaac catgcaggag attaacactc tgctgatcgc cctggataaa 4801 acatgggatg acgacttatt gccgctctgt tcccagatat ttcgccgcga cattcgtgca 4861 tcgtcagaac tgacacaggc cgaagcagta aaagctcttg gattcctgaa acagaaagcc 4921 gcagagcaga aggtggcagc atgacaccgg acattatcct gcagcgtacc gggatcgatg 4981 tgagagctgt cgaacagggg gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg 5041 cttcagaagt tcacaacgtg atagcaaaac cccgctccgg aaagaagtgg cctgacatga 5101 aaatgtccta cttccacacc ctgcttgctg aggtttgcac cggtgtggct ccggaagtta 5161 acgctaaagc actggcctgg ggaaaacagt acgagaacga cgccagaacc ctgtttgaat 5221 tcacttccgg cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgta 5281 ccgcctgctc tcccgatggt ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt 5341 ttacctcccg ggatttcatg aagttccggc tcggtggttt cgaggccata aagtcagctt 5401 acatggccca ggtgcagtac agcatgtggg tgacgcgaaa aaatgcctgg tactttgcca 5461 actatgaccc gcgtatgaag cgtgaaggcc tgcattatgt cgtgattgag cgggatgaaa 5521 agtacatggc gagttttgac gagatcgtgc cggagttcat cgaaaaaatg gacgaggcac 5581 tggctgaaat tggttttgta tttggggagc aatggcgatg acgcatcctc acgataatat 5641 ccgggtaggc gcaatcactt tcgtctactc cgttacaaag cgaggctggg tatttcccgg 5701 cctttctgtt atccgaaatc cactgaaagc acagcggctg gctgaggaga taaataataa 5761 acgaggggct gtatgcacaa agcatcttct gttgagttaa gaacttatat gcgtctattt 5821 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat 5881 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga 5941 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatatgc atagtaccga 6001 gaaactagtg cgaagtagtg atcaggtatt gctgttatct gatgagtata cgttgtcctg 6061 gccacggcag aagcacgctt atcgctccaa tttcccacaa cattagtcaa ctccgttagg 6121 cccttcattg aaagaaatga ggtcatcaaa tgtcttccaa tgtgagattt tgggccattt 6181 tttatagcaa agattgaata aggcgcattt ttcttcaaag ctttattgta cgatctgact 6241 aagttatctt ttaataattg gtattcctgt ttattgcttg aagaattgcc ggtcctattt 6301 actcgtttta ggactggttc agaattcctc aaaaattcat ccaaatatac aagtggatcg 6361 atcctacccc ttgcgctaaa gaagtatatg tgcctactaa cgcttgtctt tgtctctgtc 6421 actaaacact ggattattac tcccagatac ttattttgga ctaatttaaa tgatttcgga 6481 tcaacgttct taatatcgct gaatcttcca caattgatga aagtagctag gaagaggaat 6541 tggtataaag tttttgtttt tgtaaatctc gaagtatact caaacgaatt tagtattttc 6601 tcagtgatct cccagatgct ttcaccctca cttagaagtg ctttaagcat ttttttactg 6661 tggctatttc ccttatctgc ttcttccgat gattcgaact gtaattgcaa actacttaca 6721 atatcagtga tatcagattg atgtttttgt ccatagtaag gaataattgt aaattcccaa 6781 gcaggaatca atttctttaa tgaggcttcc agaattgttg ctttttgcgt cttgtattta 6841 aactggagtg atttattgac aatatcgaaa ctcagcgaat tgcttatgat agtattatag 6901 ctcatgaatg tggctctctt gattgctgtt ccgttatgtg taatcatcca acataaatag 6961 gttagttcag cagcacataa tgctattttc tcacctgaag gtctttcaaa cctttccaca 7021 aactgacgaa caagcacctt aggtggtgtt ttacataata taccaaattg tggcatacaa 7081 cctccttagt acatgcaacc attatcaccg ccagaggtaa aatagtcaac acgcacggtg 7141 ttagatattt atcccttgcg gtgatagatt taacgtatga gcacaaaaaa gaaaccatta 7201 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 7261 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 7321 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttacaaaa 7381 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 7441 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 7501 catgttcagg cagggatgtt ctcacctaag cttagaacct ttaccaaagg tgatgcggag 7561 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 7621 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 7681 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 7741 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 7801 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 7861 agtcagtggc ctgaagagac gtttggctga tttgttagtc ttgatgcttc actgatagat 7921 acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt 7981 tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc 8041 actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag 8101 tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg gtattttgtc 8161 accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag 8221 ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat 8281 attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt 8341 aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc 8401 tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct 8461 catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt 8521 taactggaaa agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga 8581 gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat 8641 ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc 8701 cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt 8761 ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt 8821 ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa 8881 ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc 8941 tagtcaatga taattactag tccttttcct ttgagttgtg ggtatctgta aattctgcta 9001 gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt 9061 gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa 9121 aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca 9181 gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc 9241 taaaggctta agtagcaccc tcgcaagctc ggttgcggcc gcaatcgggc aaatcgctga 9301 atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag 9361 ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta 9421 tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg 9481 cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg 9541 ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc 9601 taatgcaccc agtaaggcag cggtatcatc aacggggtct gacgctcagt ggaacgaaaa 9661 ctctttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 9721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 9781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 9841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 9901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 9961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 10021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 10081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 10141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 10201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 10261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 10321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 10381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 10441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 10501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 10561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 10621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 10681 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 10741 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 10801 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 10861 tcacgaggcc ctttcgtc //