LOCUS       BBa_K427008             1714 bp    DNA     linear       12-Mar-2026
DEFINITION  Ter PBadweak Mu Mor Ter PM GFP cassette
ACCESSION   BBa_K427008
VERSION     BBa_K427008.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     stem_loop       62..84
                     /label=stem_loop
     misc_feature    50..95
                     /label=BBa_B0011
     terminator      50..95
                     /label=BBa_B0011
     terminator      1..95
                     /label=BBa_B0014
     promoter        104..234
                     /label=promoter
     misc_feature    143..160
                     /label=AraI1
     misc_feature    164..181
                     /label=AraI2
     promoter        104..233
                     /label=BBa_K206001
     RBS             242..253
                     /label=RBS
     CDS             260..646
                     /label=MuMor
     misc_feature    242..646
                     /label=BBa_K427002
     stem_loop       662..681
                     /label=T7 TE
     misc_feature    688..688
                     /label=stop
     polyA_site      682..695
                     /label=polya
     misc_feature    655..695
                     /label=BBa_B0012
     terminator      655..695
                     /label=BBa_B0012
     stem_loop       716..738
                     /label=stem_loop
     misc_feature    704..749
                     /label=BBa_B0011
     terminator      704..749
                     /label=BBa_B0011
     terminator      655..749
                     /label=BBa_B0014
     promoter        758..828
                     /label=Pm
     promoter        758..828
                     /label=BBa_K427003
     misc_feature    242..828
                     /label=BBa_K427006
     RBS             837..851
                     /label=RBS-1\Strong
     RBS             844..848
                     /label=RBS
     misc_feature    837..851
                     /label=BBa_B0030
     RBS             837..851
                     /label=BBa_B0030
     CDS             858..1577
                     /label=GFP protein
     CDS             858..1577
                     /label=BBa_E0040
     stem_loop       1597..1640
                     /label=stem_loop
     misc_feature    1586..1665
                     /label=BBa_B0010
     terminator      1586..1665
                     /label=BBa_B0010
     stem_loop       1681..1700
                     /label=T7 TE
     misc_feature    1707..1707
                     /label=stop
     polyA_site      1701..1714
                     /label=polya
     misc_feature    1674..1714
                     /label=BBa_B0012
     terminator      1674..1714
                     /label=BBa_B0012
     misc_feature    837..1714
                     /label=BBa_E0840
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
       61 aaagccagat tattaatccg gcttttttat tattttacta gagacattga ttatttgcac
      121 ggcgtcacac tttgctatgc catagctttt ttatccataa gattagcgga tcctacctga
      181 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga
      241 gaaagaggag aaatactaga tgactgaaga cctgtttggt gatctgcaag acgataccat
      301 tctggcacac ctggataatc ctgccgagga cacaagccgt tttccggcgc tgctggctga
      361 actgaacgat ctgctgcgtg gtgagctgtc tcgcctggga gttgaccctg cccattctct
      421 ggagattgtc gtggccattt gtaaacatct gggcggaggc caagtttata ttccacgtgg
      481 acaggcactg gacagtctga ttcgtgacct gcgcatttgg aacgatttca acgggcgtaa
      541 tgtgagtgaa ctgactaccc gctatggagt gacttttaac accgtgtata aagccattcg
      601 ccgtatgcgt cgtctgaaat atcgccagta tcaaccgagc ctgctgtact agagtcacac
      661 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      721 agattattaa tccggctttt ttattatttt actagagttc tgtaaacagt aaagccggtt
      781 aatccggctt tttttacgtc ctcaatatcc tgtgatgaat aaccgtacta ctagagatta
      841 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      901 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      961 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1021 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1081 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1141 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1201 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1261 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1321 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1381 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1441 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1501 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1561 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1621 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1681 ggctcacctt cgggtgggcc tttctgcgtt tata
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