LOCUS       BBa_K398027             1406 bp    DNA     linear       05-Apr-2026
DEFINITION  LadA Protein Generator (High expression)
ACCESSION   BBa_K398027
VERSION     BBa_K398027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1406
                     /label=BBa_K398000
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccaaaa aaatccacat caacgctttc gaaatgaact
      121 gcgttggtca catcgctcac ggtctgtggc gtcacccgga aaaccagcgt caccgttaca
      181 ccgacctgaa ctactggacc gaactggctc agctgctgga aaaaggtaag ttcgacgctc
      241 tgttcctggc tgacgttgtt ggtatctacg acgtttaccg tcagtctcgt gacaccgctg
      301 ttcgtgaagc tgttcagatc ccggttaacg acccgctgat gctgatctct gctatggctt
      361 acgttaccaa acacctggct ttcgctgtta ccttctctac cacctacgaa cacccgtacg
      421 gtcacgctcg tcgtatgtct accctggacc acctgaccaa aggtcgtatc gcttggaacg
      481 ttgttacctc tcacctgccg tctgctgaca aaaacttcgg tatcaaaaaa atcctggaac
      541 acgacgaacg ttacgacctg gctgacgaat acctggaagt ttgctacaaa ctgtgggaag
      601 gttcttggga agacaacgct gttatccgtg acatcgaaaa caacatctac accgacccgt
      661 ctaaagttca cgaaatcaac cactctggta aatacttcga agttccgggt ccgcacctgt
      721 gcgaaccgtc tccgcagcgt accccggtta tctaccaggc tggtatgtct gaacgtggtc
      781 gtgagttcgc tgctaaacac gctgaatgcg ttttcctggg tggtaaagac gttgaaaccc
      841 tgaagttctt cgttgacgac atccgtaaac gtgctaaaaa atacggtcgt aacccggacc
      901 acatcaaaat gttcgctggt atctgcgtta tcgttggtaa aacccacgac gaagctatgg
      961 aaaaactgaa ctctttccag aaatactggt ctctggaagg tcacctggct cactacggtg
     1021 gtggtaccgg ctacgacctg tctaaatact cttctaacga ctacatcggt tctatctctg
     1081 ttggtgaaat catcaacaac atgtctaaac tggacggtaa atggttcaaa ctgtctgttg
     1141 gtaccccgaa aaaagttgct gacgaaatgc agtacctggt tgaagaagct ggtatcgacg
     1201 gtttcaacct ggttcagtac gtttctccgg gtaccttcgt tgacttcatc gaactggttg
     1261 ttccggaact gcaaaaacgt ggtctgtacc gtgttgacta cgaagaaggt acctaccgtg
     1321 aaaaactgtt cggtaaaggt aactaccgtc tgccggacga ccacatcgct gctcgttacc
     1381 gtaacatctc ttctaacgtt taataa
//