The alkB gene cluster from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-13 alkanes as well as that of C5-8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes n-alkanes to the respective n-alkanols and requires three soluble electron-transfer proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).
Nucleotide sequence optimized for expression in E.coli
Nucleotide sequence optimized for expression in E.coli.
Rubredoxin reductase from Gordonia sp. TF6
AlkB2, a medium-chain alkane 1-monooxygenase originating from Gordonia sp. TF6 is capable of degrading C5-13 alkanes as well as C5-8 cycloalkanes into the corresponding alkanols. It requires rubA3, rubA4 and rubR for proper functioning.
Fujii, T., Narikawa, T., Takeda, K., Kato, J., Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp. TF6. Bioscience, biotechnology, and biochemistry, 68(10) 2171-2177 (2004)
All EcoRI, PstI, SpeI and Xbal sites were removed
Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)
Alkane 1-monooxygenase from Gordonia sp. TF6
Conversion of the iGEM parts registry to SBOL2.1