BBa_K336011
type: ComponentDefinition
persistent identity: BBa K336011
display id: BBa_K336011
version: 1
was generated by: iGEM to SBOL conversion
description: p(tetR)+lasR+p(LasR)+mRFP1
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: p(tetR)+RBS+α-amylase
discontinued: false
dominant: false
experience: experience/None
group u list: _458_
m user id: 0
owner id: 5997
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Yuki Shimahara
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_C0179
component: BBa_B0034
component: BBa_E1010
component: BBa_B0032
component: BBa_R0079
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0079
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K336011_sequence/1

BBa_B0010
type: Component
persistent identity: component2288939
display id: component2288939
version: 1
top level: BBa_K336011
definition: BBa_B0010
access: public

BBa_C0179
type: Component
persistent identity: component2288938
display id: component2288938
version: 1
top level: BBa_K336011
definition: lasR
access: public

BBa_B0034
type: Component
persistent identity: component2288937
display id: component2288937
version: 1
top level: BBa_K336011
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2288956
display id: component2288956
version: 1
top level: BBa_K336011
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component2288955
display id: component2288955
version: 1
top level: BBa_K336011
definition: mRFP1
access: public

BBa_B0032
type: Component
persistent identity: component2288951
display id: component2288951
version: 1
top level: BBa_K336011
definition: BBa_B0032
access: public

BBa_R0079
type: Component
persistent identity: component2288949
display id: component2288949
version: 1
top level: BBa_K336011
definition: LasR+PAI
access: public

BBa_B0012
type: Component
persistent identity: component2288958
display id: component2288958
version: 1
top level: BBa_K336011
definition: BBa_B0012
access: public

BBa_R0040
type: Component
persistent identity: component2288931
display id: component2288931
version: 1
top level: BBa_K336011
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2288941
display id: component2288941
version: 1
top level: BBa_K336011
definition: BBa_B0012
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2288931
display id: annotation2288931
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288931/range2288931/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K336011/annotation2288931/range2288931/1
type: Range
persistent identity: range2288931
display id: range2288931
version: 1
top level: BBa_K336011
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2288937
display id: annotation2288937
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288937/range2288937/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K336011/annotation2288937/range2288937/1
type: Range
persistent identity: range2288937
display id: range2288937
version: 1
top level: BBa_K336011
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2288938
display id: annotation2288938
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288938/range2288938/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K336011/annotation2288938/range2288938/1
type: Range
persistent identity: range2288938
display id: range2288938
version: 1
top level: BBa_K336011
direction: direction/reverse
start: 81
end: 803
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2288939
display id: annotation2288939
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288939/range2288939/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K336011/annotation2288939/range2288939/1
type: Range
persistent identity: range2288939
display id: range2288939
version: 1
top level: BBa_K336011
direction: direction/forward
start: 812
end: 891
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2288941
display id: annotation2288941
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288941/range2288941/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336011/annotation2288941/range2288941/1
type: Range
persistent identity: range2288941
display id: range2288941
version: 1
top level: BBa_K336011
direction: direction/forward
start: 900
end: 940
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2288949
display id: annotation2288949
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288949/range2288949/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K336011/annotation2288949/range2288949/1
type: Range
persistent identity: range2288949
display id: range2288949
version: 1
top level: BBa_K336011
direction: direction/reverse
start: 949
end: 1105
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2288951
display id: annotation2288951
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288951/range2288951/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K336011/annotation2288951/range2288951/1
type: Range
persistent identity: range2288951
display id: range2288951
version: 1
top level: BBa_K336011
direction: direction/forward
start: 1114
end: 1126
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2288955
display id: annotation2288955
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288955/range2288955/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K336011/annotation2288955/range2288955/1
type: Range
persistent identity: range2288955
display id: range2288955
version: 1
top level: BBa_K336011
direction: direction/reverse
start: 1133
end: 1838
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2288956
display id: annotation2288956
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288956/range2288956/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K336011/annotation2288956/range2288956/1
type: Range
persistent identity: range2288956
display id: range2288956
version: 1
top level: BBa_K336011
direction: direction/forward
start: 1847
end: 1926
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2288958
display id: annotation2288958
version: 1
top level: BBa_K336011
location: https://synbiohub.org/public/igem/BBa_K336011/annotation2288958/range2288958/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K336011/annotation2288958/range2288958/1
type: Range
persistent identity: range2288958
display id: range2288958
version: 1
top level: BBa_K336011
direction: direction/forward
start: 1935
end: 1975
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (LasR & PAI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Binding region for LasR protein (positive regulation)
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
top level: LasR+PAI
direction: direction/forward
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
top level: LasR+PAI
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
top level: LasR+PAI
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
top level: LasR+PAI
direction: direction/forward
start: 140
end: 145
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

https://synbiohub.org/public/igem/BBa_K336011_sequence/1
type: Sequence
persistent identity: BBa K336011 sequence
display id: BBa_K336011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0079_sequence/1
type: Sequence
persistent identity: BBa R0079 sequence
display id: BBa_R0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z