##gff-version 3 ##sequence-region BBa_K323004 1 807 BBa_K323004 . engineered_region 121 405 . + 0 ID=annotation2102415;Name=BBa_K323037 BBa_K323004 . ribosome_entry_site 76 82 . + 0 ID=annotation2102410;Name=RBS BBa_K323004 . stop_codon 748 750 . + 0 ID=annotation2102421;Name=STOP BBa_K323004 . operator 20 44 . + 0 ID=annotation2102409;Name=lacO BBa_K323004 . start_codon 118 120 . + 0 ID=annotation2102414;Name=ATG BBa_K323004 . stop_codon 757 759 . + 0 ID=annotation2102422;Name=STOP BBa_K323004 . start_codon 88 90 . + 0 ID=annotation2102411;Name=ATG BBa_K323004 . engineered_region 412 723 . + 0 ID=annotation2102418;Name=BBa_K323108 BBa_K323004 . sequence_feature 376 405 . + 0 ID=annotation2102417;Name=linker BBa_K323004 . tag 91 111 . + 0 ID=annotation2102413;Name=Tyr + His tag BBa_K323004 . promoter 1 17 . + 0 ID=annotation2102407;Name=pT7 BBa_K323004 . sequence_feature 694 723 . + 0 ID=annotation2102424;Name=linker BBa_K323004 . sequence_feature 760 807 . + 0 ID=annotation2102423;Name=T7 term BBa_K323004 . tag 730 747 . + 0 ID=annotation2102419;Name=His tag >BBa_K323004 taatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaagaagga ggtaaataatgtatcatcaccatcaccatcacactagaatggccgacaagcagaagaacggcatcaaggccaacttcaa gatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggcccc gtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatgg tcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggtggctctggcggtgggag tgggggaagcactagagcaagtgatgatcgtccgtatgcatgtccggttgaaagctgtgatcgtcgttttagccgtagt gatgaactgacccgtcatattcgtattcatacaggtcagaaaccttttcagtgtcgtatttgcatgcgtaattttagct caagagatgttctgcgtcgtcataatcgtacccatacgggtgaaaaaccgtttgcatgtgatatttgcggtcgtaaatt tgcaagtcgtgacgtgctgcgtcgccataaccgcattcatctgcgtcagaatgatctggaaggtggtagcggtggtggt tcaggtggtagtactagacaccaccaccaccaccactaaactagataactagcataaccccttggggcctctaaacggg tcttgaggggttttttg