##gff-version 3
##sequence-region BBa_K233002 1 4131
BBa_K233002	.	terminator	4091	4131	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K233002	.	engineered_region	4091	4131	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233002	.	stop_codon	4124	4124	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233002	.	polyA_site	4118	4131	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233002	.	stem_loop	4098	4117	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233002	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K233002	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K233002	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K233002	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K233002	.	engineered_region	65	187	.	+	0	ID=BBa_K233306;Name=BBa_K233306
BBa_K233002	.	CDS	3275	3994	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K233002	.	CDS	3275	3994	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K233002	.	promoter	1	35	.	+	0	ID=BBa_J23118;Name=BBa_J23118
BBa_K233002	.	terminator	4003	4082	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K233002	.	stem_loop	4014	4057	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233002	.	engineered_region	4003	4082	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233002	.	CDS	194	3268	.	+	0	ID=BBa_I732005;Name=BBa_I732005
BBa_K233002	.	point_mutation	3217	3217	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K233002	.	stop_codon	3266	3268	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K233002	.	start_codon	194	196	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K233002	.	CDS	194	3268	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
>BBa_K233002
ttgacggctagctcagtcctaggtattgtgctagctactagagattaaagaggagaaatactagatgcagtacaaagac
gaaaacggtgttaatgagccgtctcgtcgccgtctgctgaaggttatcggcgcgctggctctggcaggttcctgcccgg
tggcgcatgcgcagaaaactcaatctgcctactagatgaccatgattacggattcactggccgtcgttttacaacgtcg
tgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa
gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccag
aagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgca
cggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccg
acgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcg
ttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttga
cctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaa
gatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatt
tccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcg
tgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaa
attatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccg
aaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgt
cggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgt
cacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaaca
actttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgt
ggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcg
atgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaat
caggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaagg
cggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggct
gtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacg
cgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgt
ctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggc
gatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaag
caaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtca
tagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtc
gctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtac
gcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaa
cctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctg
ggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaacaactgctga
cgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaa
cgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggcagatacactt
gctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctacc
ggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattgg
cctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgc
cttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttcccgagcgaaaacg
gtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaacatcagccgcta
cagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgacggt
ttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgccggtcgctacc
attaccagttggtctggtgtcaaaaataatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattct
tgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaa
cttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtg
ttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtaca
ggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtt
aatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaaga
tggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccat
tacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacag
ctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata