LOCUS BBa_K2150028 4916 bp DNA linear 04-Apr-2026 DEFINITION pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT ACCESSION BBa_K2150028 VERSION BBa_K2150028.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 2578..2605 /label=putative E. coli promoter CDS 81..2735 /label=BBa_I2032 stem_loop 2757..2776 /label=T7 TE misc_feature 2783..2783 /label=stop misc_feature 2780..2780 /label=C misc_feature 2753..2753 /label=C polyA_site 2744..2749 /label='' polyA_site 2777..2790 /label=polya misc_feature 2744..2790 /label=BBa_B0013 terminator 2744..2790 /label=BBa_B0013 promoter 2799..2818 /label=BBa_K2150031 misc_feature 2831..2834 /label=conserved RBS 2827..2838 /label=BBa_B0034 CDS 2845..4015 /label=TetX misc_feature 4016..4059 /label=protein linker CDS 4060..4779 /label=GFP CDS 2845..4779 /label=BBa_K2150013 stem_loop 4799..4842 /label=stem_loop misc_feature 4788..4867 /label=BBa_B0010 terminator 4788..4867 /label=BBa_B0010 stem_loop 4883..4902 /label=T7 TE misc_feature 4909..4909 /label=stop polyA_site 4903..4916 /label=polya misc_feature 4876..4916 /label=BBa_B0012 terminator 4876..4916 /label=BBa_B0012 terminator 4788..4916 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaacacga ttaacatcgc taagaacgac ttctctgaca 121 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 181 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 241 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 301 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 361 ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg 421 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 481 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 541 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 601 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 661 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 721 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 781 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 841 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 901 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 961 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 1021 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 1081 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 1141 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 1201 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 1261 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 1321 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 1381 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 1441 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 1501 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 1561 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 1621 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 1681 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 1741 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 1801 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 1861 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 1921 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 1981 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 2041 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 2101 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 2161 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 2221 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 2281 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 2341 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 2401 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 2461 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 2521 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 2581 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 2641 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 2701 acatcttaga gtcggacttc gcgttcgcgt aataatacta gagaaaaaat caaactggct 2761 caccttcggg tgggcctttt tgcgtttata tactagagta atacgactca ctatagggta 2821 ctagagaaag aggagaaata ctagatgaaa aaaaccatgc gtatcgacac cgacaaacag 2881 atgaacctgc tgtctgacaa aaacgttgcg atcatcggtg gtggtccggt tggtctgacc 2941 atggcgaaac tgcttcagca gaacggtatc gacgtttctg tttacgaacg tgacaacgac 3001 cgtgaagcgc gtatcttcgg tggtaccctg gacctgcaca aaggttctgg tcaggaagcg 3061 atgaaaaaag cgggtctgct tcagacctac tacgacctgg cgctgccgat gggtgttaac 3121 atcgcggaca aaaaaggtaa catcctgtct accaaaaacg ttaaaccgga aaaccgtttc 3181 gacaacccgg aaatcaaccg taacgacctg cgtgcgatcc tgctgaactc tctggaaaac 3241 gacaccgtta tctgggaccg taaactggtt atgctggaac cgggtaaaaa aaaatggacc 3301 ctgaccttcg aaaacaaacc gtctgaaacc gcggacctgg ttatcctggc gaacggtggt 3361 atgtctaaag ttcgtaaatt cgttaccgac accgaagttg aagaaaccgg taccttcaac 3421 atccaggcgg acatccacca gccggaaatc aactgcccgg gtttcttcca gctgtgcaac 3481 ggtaaccgtc tgatggcgtc tcaccagggt aacctgctgt tcgcgaaccc gaacaacaac 3541 ggtgcgctgc acttcggtat ctctttcaaa accccggacg aatggaaaaa ccagacccag 3601 gttgacttcc agaaccgtaa ctctgttgtt gacttcctgc tgaaagagtt ctctgactgg 3661 gacgaacgtt acaaagaact gatccacacc accctgtctt tcgttggtct ggcgacccgt 3721 atcttcccgc tggaaaaacc gtggaaatct aaacgtccgc tgccgatcac catgatcggt 3781 gacgcggcgc acctgatgcc gccgttcgcg ggtcagggtg ttaactctgg tctggttgac 3841 gcgctgatcc tgtctgacaa cctggcggac ggtaaattca actctatcga agaagcggtt 3901 aaaaactacg aacagcagat gttcatgtac ggtaaagaag cgcaggaaga atctacccag 3961 aacgaaatcg aaatgttcaa accggacttc accttccagc agctgctgaa cgttggtgga 4021 ggaggttctg gaggcggtgg aagtggtggc ggaggtagca tgcgtaaagg agaagaactt 4081 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt 4141 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt 4201 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt 4261 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc 4321 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag 4381 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt 4441 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca 4501 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt 4561 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca 4621 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt 4681 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct 4741 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat 4801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //