##gff-version 3
##sequence-region BBa_K2145002 1 2014
BBa_K2145002	.	stem_loop	952	995	.	+	0	ID=annotation2511881;Name=stem_loop
BBa_K2145002	.	engineered_region	1	1069	.	+	0	ID=annotation2511886;Name=BBa_J04450
BBa_K2145002	.	polyA_site	2001	2014	.	+	0	ID=annotation2511899;Name=polya
BBa_K2145002	.	engineered_region	1158	1877	.	+	0	ID=annotation2511894;Name=BBa_E0040
BBa_K2145002	.	engineered_tag	908	932	.	+	0	ID=annotation2511879;Name=barcode
BBa_K2145002	.	engineered_region	1974	2014	.	+	0	ID=annotation2511897;Name=BBa_B0012
BBa_K2145002	.	CDS	227	901	.	+	0	ID=annotation2511878;Name=mrfp1
BBa_K2145002	.	non_covalent_binding_site	1078	1096	.	+	0	ID=annotation2511887;Name=TetR 1
BBa_K2145002	.	engineered_region	1029	1069	.	+	0	ID=annotation2511882;Name=BBa_B0012
BBa_K2145002	.	promoter	1120	1125	.	+	0	ID=annotation2511891;Name=-10
BBa_K2145002	.	promoter	137	142	.	+	0	ID=annotation2511871;Name=-35
BBa_K2145002	.	engineered_region	1886	1965	.	+	0	ID=annotation2511895;Name=BBa_B0010
BBa_K2145002	.	stem_loop	1036	1055	.	+	0	ID=annotation2511883;Name=T7 TE
BBa_K2145002	.	engineered_region	941	1020	.	+	0	ID=annotation2511880;Name=BBa_B0010
BBa_K2145002	.	stop_codon	1062	1062	.	+	0	ID=annotation2511885;Name=stop
BBa_K2145002	.	promoter	161	166	.	+	0	ID=annotation2511872;Name=-10
BBa_K2145002	.	engineered_region	227	907	.	+	0	ID=annotation2511877;Name=BBa_E1010
BBa_K2145002	.	start_codon	173	173	.	+	0	ID=annotation2511874;Name=start
BBa_K2145002	.	stem_loop	1981	2000	.	+	0	ID=annotation2511898;Name=T7 TE
BBa_K2145002	.	promoter	1097	1102	.	+	0	ID=annotation2511889;Name=-35
BBa_K2145002	.	engineered_region	1078	1131	.	+	0	ID=annotation2511888;Name=BBa_R0040
BBa_K2145002	.	engineered_region	1	200	.	+	0	ID=annotation2511869;Name=BBa_R0010
BBa_K2145002	.	engineered_region	1140	1151	.	+	0	ID=annotation2511892;Name=BBa_B0034
BBa_K2145002	.	non_covalent_binding_site	1103	1121	.	+	0	ID=annotation2511890;Name=TetR 2
BBa_K2145002	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2511873;Name=LacI
BBa_K2145002	.	engineered_region	1078	2014	.	+	0	ID=annotation2511901;Name=BBa_I13522
BBa_K2145002	.	stop_codon	2007	2007	.	+	0	ID=annotation2511900;Name=stop
BBa_K2145002	.	stem_loop	1897	1940	.	+	0	ID=annotation2511896;Name=stem_loop
BBa_K2145002	.	polyA_site	1056	1069	.	+	0	ID=annotation2511884;Name=polya
BBa_K2145002	.	engineered_region	209	220	.	+	0	ID=annotation2511875;Name=BBa_B0034
BBa_K2145002	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2511870;Name=CAP
BBa_K2145002	.	conserved_region	1144	1147	.	+	0	ID=annotation2511893;Name=conserved
BBa_K2145002	.	conserved_region	213	216	.	+	0	ID=annotation2511876;Name=conserved
>BBa_K2145002
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccc
tatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata