LOCUS       BBa_K214001              731 bp    DNA     linear       15-Apr-2026
DEFINITION  Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP
ACCESSION   BBa_K214001
VERSION     BBa_K214001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=Pconst (J23119)
     misc_feature    588..593
                     /label=Mlu I
     misc_feature    594..638
                     /label=Linker (K157013-modified)
     misc_feature    639..644
                     /label=BamHI
     misc_feature    645..731
                     /label=ANP (K214002)
     misc_feature    546..587
                     /label=Dual TEV cut site (K128002)
     misc_feature    495..539
                     /label=Linker (K157013-modified)
     misc_feature    489..494
                     /label=Sal I
     misc_feature    147..488
                     /label=OmpA (J36837)
     misc_feature    54..140
                     /label=Lpp (J36835)
     misc_feature    48..53
                     /label=Hind III
     misc_feature    36..47
                     /label=RBS (B0034)
     misc_feature    540..545
                     /label=Xho I
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcaaaga ggagaaaaag cttatgaaag
       61 ctactaaact ggtactgggc gcggtaatcc tgggttctac tctgctggca ggttgctcca
      121 gcaacgctaa aatcgatcag actagaaacc cgtatgttgg ctttgaaatg ggttacgact
      181 ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg tgcatacaaa gctcagggcg
      241 ttcaactgac cgctaaactg ggttacccaa tcactgacga cctggacatc tacactcgtc
      301 tgggtggcat ggtatggcgt gcagacacta aatccaacgt ttatggtaaa aaccacgaca
      361 ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc gatcactcct gaaatcgcta
      421 cccgtctgga ataccagtgg accaacaaca tcggtgacgc acacaccatc ggcactcgtc
      481 cggacaacgt cgacggtgga ggaggttctg gaggcggtgg aagtggtggc ggaggtagcc
      541 tcgaggaaaa cctgtacttc cagggtgaaa acctgtactt ccagggtacg cgtggtggag
      601 gaggttctgg aggcggtgga agtggtggcg gaggtagcgg atccagcctg cggagatcca
      661 gctgcttcgg gggcaggatg gacaggattg gagcccagag cggactgggc tgtaacagct
      721 tccggtacta a
//