Latex Oper
type: ComponentDefinition
persistent identity: BBa K2027039
display id: BBa_K2027039
version: 1
was generated by: iGEM to SBOL conversion
description: Rubber Transferases + Small Rubber Particle Protein Cassette
created: 2016-10-12T11:00:00Z
modified: 2016-10-25T09:32:42Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/Works
group u list: _2494_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: TBA
star: false
creator: Gordon Sun, Taylor Sihavong, Michael Becich
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_Z0251
component: BBa_K2027053
component: BBa_B0034
component: BBa_K2027073
component: BBa_K2027052
component: BBa_K2027054
component: BBa_K2027055
component: BBa_K2027074
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_Z0251
sequence annotation: BBa_B0034
sequence annotation: BBa_K2027053
sequence annotation: BBa_K2027052
sequence annotation: BBa_K2027073
sequence annotation: BBa_K2027054
sequence annotation: BBa_K2027074
sequence annotation: BBa_K2027055
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K2027039_sequence/1

BBa_Z0251
type: Component
persistent identity: component2530213
display id: component2530213
version: 1
top level: Latex Oper
definition: BBa_Z0251
access: public

BBa_K2027053
type: Component
persistent identity: component2530216
display id: component2530216
version: 1
top level: Latex Oper
definition: BBa_K2027053
access: public

BBa_B0034
type: Component
persistent identity: component2530215
display id: component2530215
version: 1
top level: Latex Oper
definition: BBa_B0034
access: public

BBa_K2027073
type: Component
persistent identity: component2530218
display id: component2530218
version: 1
top level: Latex Oper
definition: BBa_K2027073
access: public

BBa_K2027052
type: Component
persistent identity: component2530217
display id: component2530217
version: 1
top level: Latex Oper
definition: BBa_K2027052
access: public

BBa_K2027052
type: Component
persistent identity: component2530220
display id: component2530220
version: 1
top level: Latex Oper
definition: BBa_K2027052
access: public

BBa_K2027054
type: Component
persistent identity: component2530219
display id: component2530219
version: 1
top level: Latex Oper
definition: BBa_K2027054
access: public

BBa_K2027055
type: Component
persistent identity: component2530222
display id: component2530222
version: 1
top level: Latex Oper
definition: BBa_K2027055
access: public

BBa_K2027074
type: Component
persistent identity: component2530221
display id: component2530221
version: 1
top level: Latex Oper
definition: BBa_K2027074
access: public

BBa_B0010
type: Component
persistent identity: component2530224
display id: component2530224
version: 1
top level: Latex Oper
definition: BBa_B0010
access: public

BBa_K2027052
type: Component
persistent identity: component2530223
display id: component2530223
version: 1
top level: Latex Oper
definition: BBa_K2027052
access: public

BBa_B0012
type: Component
persistent identity: component2530226
display id: component2530226
version: 1
top level: Latex Oper
definition: BBa_B0012
access: public

BBa_Z0251
type: SequenceAnnotation
persistent identity: annotation2530213
display id: annotation2530213
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530213/range2530213/1
component: BBa_Z0251

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530213/range2530213/1
type: Range
persistent identity: range2530213
display id: range2530213
version: 1
top level: Latex Oper
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2530215
display id: annotation2530215
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530215/range2530215/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530215/range2530215/1
type: Range
persistent identity: range2530215
display id: range2530215
version: 1
top level: Latex Oper
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K2027053
type: SequenceAnnotation
persistent identity: annotation2530216
display id: annotation2530216
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530216/range2530216/1
component: BBa_K2027053

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530216/range2530216/1
type: Range
persistent identity: range2530216
display id: range2530216
version: 1
top level: Latex Oper
direction: direction/reverse
start: 62
end: 673
orientation: inline

BBa_K2027052
type: SequenceAnnotation
persistent identity: annotation2530217
display id: annotation2530217
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530217/range2530217/1
component: BBa_K2027052

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530217/range2530217/1
type: Range
persistent identity: range2530217
display id: range2530217
version: 1
top level: Latex Oper
direction: direction/reverse
start: 682
end: 753
orientation: inline

BBa_K2027073
type: SequenceAnnotation
persistent identity: annotation2530218
display id: annotation2530218
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530218/range2530218/1
component: BBa_K2027073

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530218/range2530218/1
type: Range
persistent identity: range2530218
display id: range2530218
version: 1
top level: Latex Oper
direction: direction/reverse
start: 762
end: 789
orientation: inline

BBa_K2027054
type: SequenceAnnotation
persistent identity: annotation2530219
display id: annotation2530219
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530219/range2530219/1
component: BBa_K2027054

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530219/range2530219/1
type: Range
persistent identity: range2530219
display id: range2530219
version: 1
top level: Latex Oper
direction: direction/reverse
start: 796
end: 1665
orientation: inline

BBa_K2027052
type: SequenceAnnotation
persistent identity: annotation2530220
display id: annotation2530220
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530220/range2530220/1
component: BBa_K2027052

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530220/range2530220/1
type: Range
persistent identity: range2530220
display id: range2530220
version: 1
top level: Latex Oper
direction: direction/reverse
start: 1674
end: 1745
orientation: inline

BBa_K2027074
type: SequenceAnnotation
persistent identity: annotation2530221
display id: annotation2530221
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530221/range2530221/1
component: BBa_K2027074

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530221/range2530221/1
type: Range
persistent identity: range2530221
display id: range2530221
version: 1
top level: Latex Oper
direction: direction/reverse
start: 1754
end: 1779
orientation: inline

BBa_K2027055
type: SequenceAnnotation
persistent identity: annotation2530222
display id: annotation2530222
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530222/range2530222/1
component: BBa_K2027055

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530222/range2530222/1
type: Range
persistent identity: range2530222
display id: range2530222
version: 1
top level: Latex Oper
direction: direction/reverse
start: 1786
end: 2637
orientation: inline

BBa_K2027052
type: SequenceAnnotation
persistent identity: annotation2530223
display id: annotation2530223
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530223/range2530223/1
component: BBa_K2027052

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530223/range2530223/1
type: Range
persistent identity: range2530223
display id: range2530223
version: 1
top level: Latex Oper
direction: direction/reverse
start: 2646
end: 2717
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2530224
display id: annotation2530224
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530224/range2530224/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530224/range2530224/1
type: Range
persistent identity: range2530224
display id: range2530224
version: 1
top level: Latex Oper
direction: direction/forward
start: 2726
end: 2805
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2530226
display id: annotation2530226
version: 1
top level: Latex Oper
location: https://synbiohub.org/public/igem/BBa_K2027039/annotation2530226/range2530226/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2027039/annotation2530226/range2530226/1
type: Range
persistent identity: range2530226
display id: range2530226
version: 1
top level: Latex Oper
direction: direction/forward
start: 2814
end: 2854
orientation: inline

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: iGEM to SBOL conversion
description: T7 strong promoter
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
top level: BBa_Z0251
direction: direction/forward
start: 6
end: 28
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K2027052
type: ComponentDefinition
persistent identity: BBa K2027052
display id: BBa_K2027052
version: 1
was generated by: iGEM to SBOL conversion
description: Flag-Lumio-HisTag
created: 2016-10-23T11:00:00Z
modified: 2016-10-24T01:41:34Z
mutable provenance: See BBa_K2027000
owned by: james
owned by: myers
mutable description: Scar sequence between T7 Promoter and RBS: TACTAGAG
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Necessary spacing
star: false
creator: Michael Becich
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_K2027052_sequence/1

BBa_K2027053
type: ComponentDefinition
persistent identity: BBa K2027053
display id: BBa_K2027053
version: 1
was generated by: iGEM to SBOL conversion
description: H. Brasiliensis SRPP cDNA (codon Optimized)
created: 2016-10-23T11:00:00Z
modified: 2016-10-24T02:36:47Z
mutable provenance: Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)
owned by: james
owned by: myers
mutable description: This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation. Characteristics: ??? 612bp ORF ??? 63-bp 59-UTR ??? 181-bp 39-UTR ??? 18bp poly A tail ??? Localized in the cytoplasm
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 31619
owner id: 31619
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This sequence has been codon optimized for transfection into E. coli. Mutation A486T was induced to remove a restriction site. A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.
star: false
creator: Gordon Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2027053_sequence/1

BBa_K2027054
type: ComponentDefinition
persistent identity: BBa K2027054
display id: BBa_K2027054
version: 1
was generated by: iGEM to SBOL conversion
description: H. Brasiliensis HRT1 cDNA (codon Optimized)
created: 2016-10-23T11:00:00Z
modified: 2016-10-24T02:36:40Z
mutable provenance: Sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB061234?report=GenBank)
owned by: james
owned by: myers
mutable description: This sequence contains the Hevea brasiliensis??HRT1??mRNA for cis-prenyltransferase, complete cds. The sequence has been codon optimized for expression in E. coli. HRT1 is a cis-prenyltransferase and is responsible for initialization and elongation of polyisoprene polymers. It takes in IPP or DMAPP and polymerizes it to a polyisoprene chain.
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 31619
owner id: 31619
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This sequence has been codon optimized for expression in E. coli. No other modifications have been made to the sequence.
star: false
creator: Gordon Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2027054_sequence/1

BBa_K2027073
type: ComponentDefinition
persistent identity: BBa K2027073
display id: BBa_K2027073
version: 1
was generated by: iGEM to SBOL conversion
description: (pMS-AFa-DEa) pAKP444 RBS 1 extraction
created: 2016-10-24T11:00:00Z
modified: 2016-10-25T07:42:54Z
mutable provenance: Sequence was sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
owned by: james
owned by: myers
mutable description: (pMS-AFa-DEa) pAKP444 RBS 1 extraction Ribosome binding site extraction out of pAKP444 plasmid
discontinued: false
dominant: false
group u list: _2494_
m user id: 31619
owner id: 31619
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No alterations were made to the sequence.
star: false
creator: Gordon Sun
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K2027073_sequence/1

BBa_K2027074
type: ComponentDefinition
persistent identity: BBa K2027074
display id: BBa_K2027074
version: 1
was generated by: iGEM to SBOL conversion
description: (pMS-AFa-DEa) pAKP444 RBS 2 extraction
created: 2016-10-24T11:00:00Z
modified: 2016-10-25T08:13:34Z
mutable provenance: Sequence sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
owned by: james
owned by: myers
mutable description: (pMS-AFa-DEa) pAKP444 RBS 2extraction ribosome binding site extracted from plasmid pAKP444.
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 31619
owner id: 31619
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No alterations were made to the sequence.
star: false
creator: Gordon Sun
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K2027074_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K2027055
type: ComponentDefinition
persistent identity: BBa K2027055
display id: BBa_K2027055
version: 1
was generated by: iGEM to SBOL conversion
description: H. Brasiliensis HRT2 cis-prenyltransferase
created: 2016-10-23T11:00:00Z
modified: 2016-10-24T02:36:28Z
mutable provenance: Sequence was sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB064661?report=GenBank)
owned by: james
owned by: myers
mutable description: This sequence codes for a cis-prenyltransferase responsible for polymerization of IPP and DMAPP into polyisoprene compounds. This sequence is from the rubber tree, Hevea brasiliensis
discontinued: false
dominant: false
experience: experience/None
group u list: _2494_
m user id: 31619
owner id: 31619
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This sequence has been acquired from H. brasiliensis and codon optimized for transfection into E. coli. No other modifications have been made to the sequence.
star: false
creator: Gordon Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2027055_sequence/1

https://synbiohub.org/public/igem/BBa_K2027039_sequence/1
type: Sequence
persistent identity: BBa K2027039 sequence
display id: BBa_K2027039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatcatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaattaaccattactagatggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaaactatatactagatggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0251_sequence/1
type: Sequence
persistent identity: BBa Z0251 sequence
display id: BBa_Z0251_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027052_sequence/1
type: Sequence
persistent identity: BBa K2027052 sequence
display id: BBa_K2027052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027053_sequence/1
type: Sequence
persistent identity: BBa K2027053 sequence
display id: BBa_K2027053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027054_sequence/1
type: Sequence
persistent identity: BBa K2027054 sequence
display id: BBa_K2027054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027073_sequence/1
type: Sequence
persistent identity: BBa K2027073 sequence
display id: BBa_K2027073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataaggtaccaggaggaattaaccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027074_sequence/1
type: Sequence
persistent identity: BBa K2027074 sequence
display id: BBa_K2027074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataaggtaccaggaggaaactata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2027055_sequence/1
type: Sequence
persistent identity: BBa K2027055 sequence
display id: BBa_K2027055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactgttatgagttaggagttaaatatgctacaatttacgccttctcgattgataatttccgccgtaaaccgcacgaggtacagtatgttatgaacttgatgttagagaaaatcgagggcatgatcatggaagaatcaattattaacgcctatgatatttgcgtgcgttttgtggggaatcttaaacttttggatgagccattgaaaacggcggcagataagatcatgcgtgcgacagccaaaaattctaaatttgttctgctgcttgcagtttgctatacatcgacagacgagatcgtacacgctgtggaagaatcttctaaggacaagctgaaatcggatgagatttgcaatgacggcaacggtgactgcgtaattaagattgaagagatggagccatattctgaaattaagctggttgagttggagcgcaatacctacatcaacccgtatcctgatgtcttaattcgcacaagcggggaaacgcgcttgagcaactacctgttgtggcaaacaactaactgtatcctttattctccacatgctttatggcctgaaattggcttgcgccatgtcgtctgggcggttattaactgccagcgtcactacagctatttggagaaacataaggaatacctgaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z