Rubber Transferases + Small Rubber Particle Protein Cassette
Gordon Sun, Taylor Sihavong, Michael Becich
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Scar sequence between T7 Promoter and RBS: TACTAGAG
H. Brasiliensis SRPP cDNA (codon Optimized)
Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)
This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation. Characteristics: ??? 612bp ORF ??? 63-bp 59-UTR ??? 181-bp 39-UTR ??? 18bp poly A tail ??? Localized in the cytoplasm
This sequence has been codon optimized for transfection into E. coli. Mutation A486T was induced to remove a restriction site. A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.
H. Brasiliensis HRT1 cDNA (codon Optimized)
Sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB061234?report=GenBank)
This sequence contains the Hevea brasiliensis??HRT1??mRNA for cis-prenyltransferase, complete cds. The sequence has been codon optimized for expression in E. coli. HRT1 is a cis-prenyltransferase and is responsible for initialization and elongation of polyisoprene polymers. It takes in IPP or DMAPP and polymerizes it to a polyisoprene chain.
This sequence has been codon optimized for expression in E. coli. No other modifications have been made to the sequence.
Sequence was sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
(pMS-AFa-DEa) pAKP444 RBS 1 extraction Ribosome binding site extraction out of pAKP444 plasmid
No alterations were made to the sequence.
Sequence sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
(pMS-AFa-DEa) pAKP444 RBS 2extraction ribosome binding site extracted from plasmid pAKP444.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
H. Brasiliensis HRT2 cis-prenyltransferase
Sequence was sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB064661?report=GenBank)
This sequence codes for a cis-prenyltransferase responsible for polymerization of IPP and DMAPP into polyisoprene compounds. This sequence is from the rubber tree, Hevea brasiliensis
This sequence has been acquired from H. brasiliensis and codon optimized for transfection into E. coli. No other modifications have been made to the sequence.
Conversion of the iGEM parts registry to SBOL2.1