##gff-version 3
##sequence-region BBa_K195307 1 2245
BBa_K195307	.	terminator	2117	2196	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K195307	.	engineered_region	2117	2196	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195307	.	stem_loop	2128	2171	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195307	.	terminator	2205	2245	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K195307	.	stem_loop	2212	2231	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195307	.	engineered_region	2205	2245	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195307	.	polyA_site	2232	2245	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195307	.	stop_codon	2238	2238	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195307	.	CDS	81	2108	.	+	0	ID=BBa_K195304;Name=BBa_K195304
BBa_K195307	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195307	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195307	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195307	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195307	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195307	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195307	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195307	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K195307
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaagaaaatactgacagttctatctatttttattctttcagcgtgtaatagtgacaataccagcttgaaagaaacg
gtaagctctaattctgcagatgtagtagaaacagaaacttaccaactgacaccgattgatgctcctagctcttttttat
ctcattcttgggagcaaacatgtggcacacctatcttgaatgaaagtgacaagcaagcgatatcttttgattttgttgc
tccagagttaaagcaagatgaaaagtattgttttacttttaaaggtattacaggcgatcataggtatatcacaaataca
acattaactgttgttgcacctacgctagaagtttacatcgatcatgcatccttaccatcgctacagcagcttatccaca
ttattcaagcaaaagatgaatacccaagtaatcaacgttttgtctcttggaagcgtgtaactgttgatgctgataatgc
caataagttaaacattcatacttatccattaaaaggcaataatacctcaccagaaatggtggcagcgattgatgagtat
gctcagagcaaaaatcgattgaatatagagttctatacaaatacagctcatgtttttaataatttaccacctattattc
aacctttatataataacgagaaggtgaaaatttctcatattagtttgtatgatgatggttcttctgaatatgtaagttt
atatcaatggaaagatacaccaaataagatagaaacattagaaggtgaagtatcgcttcttgctaattatttagcagga
acatctccggatgcaccaaaaggaatgggaaatcgttataactggcataaattatatgacactgattattactttttgc
gcgaagattaccttgacgttgaagcaaacctacatgatttacgtgattatttaggctcttccgcaaagcaaatgccatg
ggatgaatttgctaaattatctgattctcagcaaacactatttttagatattgtgggttttgataaagagcaattgcaa
caacaatattcacaatccccactaccaaactttatttttaccggcacaacaacttgggctgggggggaaacgaaagagt
attatgctcagcaacaagtaaatgtgattaataatgcgatcaatgaaactagcccttattatttaggtaaagactacga
tctatttttcaaggggcatcctgctggtggcgttattaacgacatcattcttggaagcttccctgatatgatcaatatt
ccagccaagatttcatttgaggtcttgatgatgacggatatgttgcctgatacagtagctggtattgcgagctctctgt
acttcacaattcctgccgataaagttaattttattgtatttacttcatctgacactattactgatcgtgaagaggctct
taaatcaccattagtacaagtgatgctaacgttgggtattgttaaagaaaaagatgttctgttctgggctgatcataaa
gtaaactcgatggaagttgccattgatgaagcctgtactcggatcattgcaaagcgacaaccaaccgcgagtgatttac
gcttggttattgctattatcaaaacaattactgatcttgagcgtattggcgatgtggcagaaagtattgctaaagtcgc
attagagagctttagtaataagcaatataacctattggtttctttagaatctcttggccagcatacggttcgaatgctg
catgaggtgttagatgcgtttgctcgtatggatgttaaagccgcaatagaagtgtaccaagaagatgatcgaattgatc
aagagtatgagtcgatagtcagacagctaatggcccatatgatggaagatccaagctcaattcctaatgtaatgaaagt
gatgtgggcggcacgttctattgagcgagtgggtgatcgctgtcaaaacatttgtgagtacattatctactttgtgaag
ggtaaagacgttcgccataccaaaccagatgattttggtactatgctcgattaatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata