##gff-version 3
##sequence-region BBa_K195109 1 1748
BBa_K195109	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K195109	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K195109	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K195109	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K195109	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K195109	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K195109	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K195109	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K195109	.	terminator	1708	1748	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K195109	.	polyA_site	1735	1748	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K195109	.	engineered_region	1708	1748	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K195109	.	stop_codon	1741	1741	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K195109	.	stem_loop	1715	1734	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K195109	.	terminator	1620	1699	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K195109	.	stem_loop	1631	1674	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K195109	.	engineered_region	1620	1699	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K195109	.	CDS	553	1611	.	+	0	ID=BBa_K195105;Name=BBa_K195105
BBa_K195109	.	tag	83	546	.	+	0	ID=BBa_K103006;Name=BBa_K103006
BBa_K195109	.	start_codon	86	88	.	+	0	ID=annotation1993480;Name=ATG;Parent=BBa_K103006
BBa_K195109	.	sequence_feature	541	546	.	+	0	ID=annotation1990002;Name=SacI;Parent=BBa_K103006
BBa_K195109	.	sequence_feature	83	89	.	+	0	ID=annotation1989999;Name=NdeI;Parent=BBa_K103006
BBa_K195109	.	CDS	86	520	.	+	0	ID=annotation1990000;Name=OmpA;Parent=BBa_K103006
BBa_K195109	.	CDS	521	546	.	+	0	ID=annotation1990001;Name=linker;Parent=BBa_K103006
>BBa_K195109
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacg
ctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaagg
cagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctg
gacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccg
gcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggac
caacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctctactaga
tggattatcaagtgtcaagtccaatctatgacatcaattattatacatcggagccctgccaaaaaatcaatgtgaagca
aatcgcagcccgcctcctgcctccgctctactcactggtgttcatctttggttttgtgggcaacatgctggtcatcctc
atcctgataaactgcaaaaggctgaagagcatgactgacatctacctgctcaacctggccatctctgacctgtttttcc
ttcttactgtccccttctgggctcactatgctgccgcccagtgggactttggaaatacaatgtgtcaactcttgacagg
gctctattttataggcttcttctctggaatcttcttcatcatcctcctgacaatcgataggtacctggctgtcgtccat
gctgtgtttgctttaaaagccaggacggtcacctttggggtggtgacaagtgtgatcacttgggtggtggctgtgtttg
cgtctctcccaggaatcatctttaccagatctcaaaaagaaggtcttcattacacctgcagctctcattttccatacag
tcagtatcaattctggaagaatttccagacattaaagatagtcatcttggggctggtcctgccgctgcttgtcatggtc
atctgctactcgggaatcctaaaaactctgcttcggtgtcgaaatgagaagaagaggcacagggctgtgaggcttatct
tcaccatcatgattgtttattttctcttctgggctccctacaacattgtccttctcctgaacaccttccaggaattctt
tggcctgaataattgcagtagctctaacaggttggaccaagctatgcaggtgacagagactcttgggatgacgcactgc
tgcatcaaccccatcatctatgcctttgtcggggagaagttcagaaactacctcttagtcttcttccaaaagcacattg
ccaaacgcttctgcaaatgctgttctattttccagcaagaggctcccgagcgagcaagctcagtttacacccgatccac
tggggagcaggaaatatctgtgggcttgtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata