##gff-version 3
##sequence-region BBa_K1896014 1 1332
BBa_K1896014	.	engineered_region	1	55	.	+	0	ID=BBa_S05355;Name=BBa_S05355
BBa_K1896014	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_S05355
BBa_K1896014	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05355
BBa_K1896014	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1896014	.	engineered_region	1204	1332	.	+	0	ID=BBa_S05347;Name=BBa_S05347
BBa_K1896014	.	terminator	1292	1332	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S05347
BBa_K1896014	.	engineered_region	1292	1332	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1896014	.	polyA_site	1319	1332	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1896014	.	stop_codon	1325	1325	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1896014	.	stem_loop	1299	1318	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1896014	.	terminator	1204	1283	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S05347
BBa_K1896014	.	engineered_region	1204	1283	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1896014	.	stem_loop	1215	1258	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1896014	.	engineered_region	62	1195	.	+	0	ID=BBa_S05360;Name=BBa_S05360
BBa_K1896014	.	CDS	62	775	.	+	0	ID=BBa_K1896006;Name=BBa_K1896006;Parent=BBa_S05360
BBa_K1896014	.	sequence_alteration	548	550	.	+	0	ID=annotation2499182;Name=V163A;Parent=BBa_K1896006
BBa_K1896014	.	start_codon	62	64	.	+	0	ID=annotation2499187;Name=start;Parent=BBa_K1896006
BBa_K1896014	.	sequence_alteration	518	520	.	+	0	ID=annotation2499181;Name=M153T;Parent=BBa_K1896006
BBa_K1896014	.	sequence_alteration	677	679	.	+	0	ID=annotation2499183;Name=A206K;Parent=BBa_K1896006
BBa_K1896014	.	sequence_alteration	683	685	.	+	0	ID=annotation2499184;Name=S208L;Parent=BBa_K1896006
BBa_K1896014	.	CDS	62	775	.	+	0	ID=annotation2499179;Name=mGFPuv2;Parent=BBa_K1896006
BBa_K1896014	.	sequence_alteration	356	358	.	+	0	ID=annotation2499180;Name=F99S;Parent=BBa_K1896006
BBa_K1896014	.	CDS	776	1195	.	+	0	ID=BBa_K1896004;Name=BBa_K1896004;Parent=BBa_S05360
BBa_K1896014	.	CDS	1172	1189	.	+	0	ID=annotation2497044;Name=His-tag;Parent=BBa_K1896004
BBa_K1896014	.	CDS	776	790	.	+	0	ID=annotation2497040;Name=GSTGS linker;Parent=BBa_K1896004
BBa_K1896014	.	stop_codon	1190	1195	.	+	0	ID=annotation2497045;Name=stop;Parent=BBa_K1896004
BBa_K1896014	.	CDS	791	1171	.	+	0	ID=annotation2497042;Name=Streptavidin;Parent=BBa_K1896004
BBa_K1896014	.	point_mutation	835	835	.	+	0	ID=annotation2497041;Name=BamHI site removed;Parent=BBa_K1896004
>BBa_K1896014
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaag
tcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcgg
acacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaac
ttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatg
gccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgacca
catggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatct
gctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctc
tgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggc
taccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacc
tggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagcca
acgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcacca
ccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata