LOCUS       BBa_K1896012            1542 bp    DNA     linear       12-Mar-2026
DEFINITION  INP_NC-streptavidin generator
ACCESSION   BBa_K1896012
VERSION     BBa_K1896012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S05357
     misc_feature    62..64
                     /label=start
     CDS             62..985
                     /label=INP_NC
     misc_feature    721..721
                     /label=BsaI site removed
     CDS             62..985
                     /label=BBa_K1896003
     CDS             986..1000
                     /label=GSTGS linker
     misc_feature    1045..1045
                     /label=BamHI site removed
     CDS             1001..1381
                     /label=Streptavidin
     CDS             1382..1399
                     /label=His-tag
     misc_feature    1400..1405
                     /label=stop
     CDS             986..1405
                     /label=BBa_K1896004
     misc_feature    62..1405
                     /label=BBa_S05358
     stem_loop       1425..1468
                     /label=stem_loop
     misc_feature    1414..1493
                     /label=BBa_B0010
     terminator      1414..1493
                     /label=BBa_B0010
     stem_loop       1509..1528
                     /label=T7 TE
     misc_feature    1535..1535
                     /label=stop
     polyA_site      1529..1542
                     /label=polya
     misc_feature    1502..1542
                     /label=BBa_B0012
     terminator      1502..1542
                     /label=BBa_B0012
     misc_feature    1414..1542
                     /label=BBa_S05347
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatgaccctg gataaagcgc tggtgctgcg cacttgtgcc aataatatgg ccgaccattg
      121 tggcctgatc tggcctgctt ctggtacagt tgaaagccgc tattggcagt ctacacgtcg
      181 tcacgaaaac gggctggttg ggctgctgtg gggtgccgga acatccgcct ttctgagcgt
      241 tcatgccgat gctcgttgga ttgtttgtga ggttgctgtt gccgacatca ttagcctgga
      301 agaaccgggt atggtgaaat ttcctcgtgc tgaagtggtc catgttggtg atcgtattag
      361 cgcctcccat ttcatttcag ctcgtcaggc tgatccagca agcacatcaa caagcacctc
      421 taccagtaca ctgacaccaa tgccaaccgc tatccctact cctatgccgg ctgtagcctc
      481 tgttactctg cctgtggccg aacaagctcg ccacgaagtc tttgacgttg cctctgtgtc
      541 tgctgctgct gctcctgtta acactctgcc tgttaccacg cctcaaaatc tgcaaacggc
      601 gacctatggc tcaacactga gtggtgataa ccattctcgt ctgattgccg ggtatggctc
      661 taacgaaacc gccggaaacc attctgatct gatcggtggt cacgattgta cgctgatggc
      721 cggtgatcaa tctcgtctga ccgctggcaa aaattccgtt ctgaccgctg gggcacgttc
      781 taaactgatc gggagcgaag gctctactct gtccgccgga gaagattcta ccctgatctt
      841 tcgtctgtgg gatggtaaac gttatcgtca gctggtcgct cgtacaggtg aaaatggtgt
      901 ggaagccgac attccgtatt atgtgaatga ggacgatgat attgtggaca aaccggacga
      961 agatgatgat tggatcgaag tgaaaggcag caccggatct gctgaagctg gtatcaccgg
     1021 cacctggtac aaccagctgg gatctacctt catcgttacc gctggtgctg acggtgctct
     1081 gaccggtacc tacgaatccg ctgttggtaa cgctgaaagc cgctacgttc tgaccggtcg
     1141 ttacgactcc gctccggcta ccgacggttc cggaaccgct ctgggttgga ccgttgcttg
     1201 gaaaaacaac taccgtaacg ctcactccgc taccacctgg tctggccagt acgttggtgg
     1261 tgctgaagct cgtatcaaca cccagtggtt gttgacctcc ggcaccaccg aagccaacgc
     1321 gtggaaatcc accctggttg gtcacgacac cttcaccaaa gttaaaccgt ccgctgcttc
     1381 ccatcaccat caccaccatt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1501 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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