LOCUS       BBa_K1893003            1871 bp    DNA     linear       25-Apr-2026
DEFINITION  Rhl receiver with GFP reporter (RhlR+pRhl+GFP)
ACCESSION   BBa_K1893003
VERSION     BBa_K1893003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..790
                     /label=BBa_C0171
     stem_loop       810..853
                     /label=stem_loop
     misc_feature    799..878
                     /label=BBa_B0010
     terminator      799..878
                     /label=BBa_B0010
     stem_loop       894..913
                     /label=T7 TE
     misc_feature    920..920
                     /label=stop
     polyA_site      914..927
                     /label=polya
     misc_feature    887..927
                     /label=BBa_B0012
     terminator      887..927
                     /label=BBa_B0012
     terminator      799..927
                     /label=BBa_B0015
     misc_feature    936..955
                     /label=RhlR
     promoter        954..959
                     /label=-35
     promoter        977..982
                     /label=-10
     misc_feature    988..988
                     /label=start
     promoter        936..988
                     /label=BBa_R0071
     misc_feature    1001..1004
                     /label=conserved
     RBS             997..1008
                     /label=BBa_B0034
     CDS             1015..1734
                     /label=GFP protein
     CDS             1015..1734
                     /label=BBa_E0040
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     misc_feature    997..1871
                     /label=BBa_I13504
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      901 cttcgggtgg gcctttctgc gtttatatac tagagtcctg tgaaatctgg cagttaccgt
      961 tagctttcga attggctaaa aagtgttcta ctagagaaag aggagaaata ctagatgcgt
     1021 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1081 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1141 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1201 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1261 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1321 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1381 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1441 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1501 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1561 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1621 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1681 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat a
//